MD17G1038600.v1.1
ERF Family

2'-5' RNA ligase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004409
Physical Location & Seq
Forward (+)
2801707 .. 2802264
558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1038600.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGCAATGTCAGAGGGCTACGCAATCGAGCTTTACTTCGATCCAGCCCTCGAAAACCAGGTCTTGAAGGCTTGGAACGTGCTCGCCCGCCGTCAGATTAGTACCCAGCTTATCGAAATCGAATCACGACCTCACATCACCCTCTTCTCAAGTCCCTTTATCGAACCCGCCAAGCTCGAAAACGTGATCAGAACCTTCGCTTCCAAGCAAGAGCCTCTGGCCTTGTCTTTCTCTTCGATCGGGAGCCTCCCAAATGACAACAACGTCCTGTTTCTGTCGCCAACTCCGTCGGTTTCGTTGCTGCAGTTTCAGTCCCAATTATGTGAGGCAATGAAGAAAGAAGGTGTGGAAATTGGGGAGGAGTATCGCACTGACTGCTGGGTTCCTTACTGTGCTGTCGCTCAGGAAGTACCCAAGACTCGAATGGCGGAGGCATTCTGCGTGTTGCGGGACTTGAAGTTGCCGGTTGCAGGGTATGCCATGGATATTGGATTGGTGGAGTTTTCACCGGTTCGCGAGCTGTTCTCGTTTGTGCTAGGTAACACAGTGGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2_5_RNA_ligase2

Protein Analysis

186

Amino Acids

20.7

Weight (kDa)

4.74

Isoelectric Point (pI)

67.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2_5_RNA_ligase2 PF13563 42 - 165 5.5e-13 2'-5' RNA ligase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
AccII CGCG 1 cut(s) 516
AciI CCGC 4 cut(s) 88, 168, 428, 448
AclWI GGATC 1 cut(s) 35
AfaI GTAC 2 cut(s) 103, 411
AfiI CCNNNNNNNGG 1 cut(s) 470
AgeI ACCGGT 1 cut(s) 508
AgsI TTSAA 2 cut(s) 67, 457
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 4 cut(s) 31, 109, 175, 520
AluI AGCT 4 cut(s) 31, 109, 175, 520
Alw21I GWGCWC 1 cut(s) 84
AlwI GGATC 1 cut(s) 35
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 1 cut(s) 301
ArsI GACNNNNNNTTYG 2 cut(s) 45, 77
AsiGI ACCGGT 1 cut(s) 508
AsuHPI GGTGA 2 cut(s) 130, 498
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 288
BceAI ACGGC 1 cut(s) 75
BciT130I CCWGG 1 cut(s) 59
BclI TGATCA 1 cut(s) 186
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 1 cut(s) 302
BisI GCNGC 1 cut(s) 302
BlsI GCNGC 1 cut(s) 303
Bme1390I CCNGG 1 cut(s) 59
BmiI GGNNCC 2 cut(s) 245, 384
BmrFI CCNGG 1 cut(s) 59
BplI GAGNNNNNCTC 2 cut(s) 509, 541
Bpu10I CCTNAGC 1 cut(s) 402
BpuEI CTTGAG 1 cut(s) 133
BsaJI CCNNGG 1 cut(s) 480
BsaWI WCCGGW 1 cut(s) 508
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse118I RCCGGY 2 cut(s) 463, 508
Bse3DI GCAATG 2 cut(s) 12, 336
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMI GCAATG 2 cut(s) 12, 336
BseMII CTCAG 1 cut(s) 416
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 1 cut(s) 288
BseYI CCCAGC 2 cut(s) 105, 378
Bsh1236I CGCG 1 cut(s) 516
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 1 cut(s) 221
BshTI ACCGGT 1 cut(s) 508
BsiEI CGRYCG 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 2 cut(s) 464, 509
BslFI GGGAC 3 cut(s) 138, 298, 464
BslI CCNNNNNNNGG 1 cut(s) 470
BsmFI GGGAC 3 cut(s) 138, 298, 464
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 3 cut(s) 40, 186, 237
Bsp19I CCATGG 1 cut(s) 480
Bsp68I TCGCGA 1 cut(s) 516
BspACI CCGC 4 cut(s) 88, 168, 428, 448
BspANI GGCC 1 cut(s) 221
BspCNI CTCAG 1 cut(s) 415
BspFNI CGCG 1 cut(s) 516
BspLI GGNNCC 2 cut(s) 245, 384
BspMAI CTGCAG 1 cut(s) 306
BspPI GGATC 1 cut(s) 35
BsrDI GCAATG 2 cut(s) 12, 336
BsrFI RCCGGY 2 cut(s) 463, 508
BssAI RCCGGY 2 cut(s) 463, 508
BssECI CCNNGG 1 cut(s) 480
BssMI GATC 3 cut(s) 40, 186, 237
BssT1I CCWWGG 1 cut(s) 480
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 2 cut(s) 392, 547
Bst6I CTCTTC 2 cut(s) 149, 238
BstAPI GCANNNNNTGC 2 cut(s) 375, 476
BstC8I GCNNGC 3 cut(s) 84, 88, 518
BstDEI CTNAG 1 cut(s) 402
BstDSI CCRYGG 1 cut(s) 480
BstFNI CGCG 1 cut(s) 516
BstKTI GATC 3 cut(s) 43, 189, 240
BstMBI GATC 3 cut(s) 40, 186, 237
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 2 cut(s) 375, 476
BstNI CCWGG 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 57
BstSFI CTRYAG 1 cut(s) 302
BstUI CGCG 1 cut(s) 516
BstV1I GCAGC 1 cut(s) 288
BsuRI GGCC 1 cut(s) 221
BtgI CCRYGG 1 cut(s) 480
BtsIMutI CAGTG 2 cut(s) 369, 552
BtuMI TCGCGA 1 cut(s) 516
Cac8I GCNNGC 3 cut(s) 84, 88, 518
Cfr10I RCCGGY 2 cut(s) 463, 508
CsiI ACCWGGT 1 cut(s) 57
Csp6I GTAC 2 cut(s) 102, 410
CspAI ACCGGT 1 cut(s) 508
CviAII CATG 2 cut(s) 481, 555
CviQI GTAC 2 cut(s) 102, 410
DdeI CTNAG 1 cut(s) 402
DpnI GATC 3 cut(s) 42, 188, 239
DpnII GATC 3 cut(s) 40, 186, 237
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
Eam1104I CTCTTC 2 cut(s) 149, 238
EarI CTCTTC 2 cut(s) 149, 238
EciI GGCGGA 1 cut(s) 443
Eco130I CCWWGG 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 480
ErhI CCWWGG 1 cut(s) 480
FaeI CATG 2 cut(s) 484, 558
FaiI YATR 4 cut(s) 322, 477, 482, 556
FaqI GGGAC 3 cut(s) 138, 298, 464
FatI CATG 2 cut(s) 480, 554
FauI CCCGC 3 cut(s) 95, 175, 441
FbaI TGATCA 1 cut(s) 186
Fnu4HI GCNGC 1 cut(s) 302
Fsp4HI GCNGC 1 cut(s) 302
FspBI CTAG 1 cut(s) 536
GluI GCNGC 1 cut(s) 302
GsaI CCCAGC 2 cut(s) 109, 382
HaeIII GGCC 1 cut(s) 221
HapII CCGG 2 cut(s) 464, 509
Hin1II CATG 2 cut(s) 484, 558
HinfI GANTC 2 cut(s) 122, 418
HpaII CCGG 2 cut(s) 464, 509
HphI GGTGA 2 cut(s) 130, 498
Hpy188I TCNGA 3 cut(s) 13, 96, 191
Hpy188III TCNNGA 5 cut(s) 64, 126, 241, 404, 515
Hpy99I CGWCG 1 cut(s) 292
HpyAV CCTTC 3 cut(s) 61, 205, 335
HpyCH4III ACNGT 2 cut(s) 392, 547
HpyCH4IV ACGT 3 cut(s) 78, 183, 264
HpyCH4V TGCA 2 cut(s) 304, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 375, 476
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 3 cut(s) 78, 183, 264
Hsp92II CATG 2 cut(s) 484, 558
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 3 cut(s) 40, 186, 237
LmnI GCTCC 1 cut(s) 243
Lsp1109I GCAGC 1 cut(s) 288
MabI ACCWGGT 1 cut(s) 57
MaeI CTAG 1 cut(s) 536
MaeII ACGT 3 cut(s) 78, 183, 264
MaeIII GTNAC 1 cut(s) 539
MalI GATC 3 cut(s) 42, 188, 239
MboI GATC 3 cut(s) 40, 186, 237
MboII GAAGA 3 cut(s) 136, 225, 346
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 2 cut(s) 317, 351
MlyI GAGTC 1 cut(s) 412
MnlI CCTC 9 cut(s) 7, 59, 141, 152, 225, 257, 319, 352, 424
MspI CCGG 2 cut(s) 464, 509
MspR9I CCNGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 434
MvaI CCWGG 1 cut(s) 59
MvnI CGCG 1 cut(s) 516
MwoI GCNNNNNNNGC 2 cut(s) 375, 476
NcoI CCATGG 1 cut(s) 480
NdeII GATC 3 cut(s) 40, 186, 237
NlaIII CATG 2 cut(s) 484, 558
NlaIV GGNNCC 2 cut(s) 245, 384
NruI TCGCGA 1 cut(s) 516
PcsI WCGNNNNNNNCGW 1 cut(s) 284
PctI GAATGC 1 cut(s) 434
PfeI GAWTC 1 cut(s) 122
PinAI ACCGGT 1 cut(s) 508
PkrI GCNGC 1 cut(s) 303
Ple19I CGATCG 1 cut(s) 240
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
Psp6I CCWGG 1 cut(s) 57
PspFI CCCAGC 2 cut(s) 105, 378
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 2 cut(s) 245, 384
PstI CTGCAG 1 cut(s) 306
PvuI CGATCG 1 cut(s) 240
RruI TCGCGA 1 cut(s) 516
RsaI GTAC 2 cut(s) 103, 411
RsaNI GTAC 2 cut(s) 102, 410
SatI GCNGC 1 cut(s) 302
Sau3AI GATC 3 cut(s) 40, 186, 237
SchI GAGTC 1 cut(s) 412
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 1 cut(s) 84
SexAI ACCWGGT 1 cut(s) 57
SfcI CTRYAG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 2 cut(s) 317, 351
SsiI CCGC 4 cut(s) 88, 168, 428, 448
SspMI CTAG 1 cut(s) 536
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 1 cut(s) 480
TaaI ACNGT 2 cut(s) 392, 547
TaiI ACGT 3 cut(s) 81, 186, 267
TaqI TCGA 9 cut(s) 27, 39, 51, 114, 120, 162, 177, 236, 421
TasI AATT 2 cut(s) 317, 351
TfiI GAWTC 1 cut(s) 122
TscAI CASTG 2 cut(s) 376, 552
TseI GCWGC 1 cut(s) 301
TspDTI ATGAA 1 cut(s) 347
TspGWI ACGGA 1 cut(s) 276
TspRI CASTG 2 cut(s) 376, 552
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.