RLG00000021972
ERF Family

2'-5' RNA ligase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004409
Physical Location & Seq
Reverse (-)
79649635 .. 79650186
552 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021972

Sequence Viewer

Length: 552 bp
ATGTCACAAGGCTATGCAATCGAGCTTTACTTCGATCCAGCTCTGGAAAACCAGGTCCTGAAGGCTTGGAATGTTCTTGCTCGCCGCCAGATTAGTACCCAGCTCATCGAAATCGAATCACGCCCTCACATCACTCTCTTCTCCAGCTCCTTCATCGAACCAGCAAAGCTCGAAAACATAATCAAGTCTTTCGCTGCAAAGCAAGAGCCTCTGGCCTTGTCTTTCTCGTCAATTGGGAGCTTCCCCACTGATAACAATGTACTGTTTCTGGCACCAACTCCTTCGGTTTCATTGCTTCAGTTCCAGGCCCAGTTGTGTGAGGCAATGAAGAAAGAAGGTGTTGAAATTGGGGAGGAGTATCGCACTGATTCTTGGATGCCTTACTGTGCTGTTGCTCAAGATGTGCCGAAGAGTCGGATGGCTGAAGCGTTTTGCGTATTGAGGGACTTGAAGTTGCCAGTTGCTGGGTATGCTATGGATATTGGGCTGGTGGAGTTTTCGCCGGTCAGGGAGTTGTTTTCTTTTGTGCTAGGTAACACAGTAGAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2_5_RNA_ligase2

Protein Analysis

184

Amino Acids

20.43

Weight (kDa)

4.78

Isoelectric Point (pI)

66.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2_5_RNA_ligase2 PF13563 40 - 163 6.8e-15 2'-5' RNA ligase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 271
AccB7I CCANNNNNTGG 1 cut(s) 464
AciI CCGC 1 cut(s) 85
AclWI GGATC 1 cut(s) 29
AcuI CTGAAG 3 cut(s) 80, 281, 444
AfaI GTAC 2 cut(s) 97, 261
AfiI CCNNNNNNNGG 1 cut(s) 464
AgsI TTSAA 2 cut(s) 344, 451
AjnI CCWGG 2 cut(s) 51, 303
AluBI AGCT 6 cut(s) 25, 41, 103, 147, 169, 240
AluI AGCT 6 cut(s) 25, 41, 103, 147, 169, 240
AlwI GGATC 1 cut(s) 29
AlwNI CAGNNNCTG 2 cut(s) 58, 464
AoxI GGCC 2 cut(s) 213, 306
ApeKI GCWGC 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 55, 307
AvaII GGWCC 1 cut(s) 55
BanI GGYRCC 1 cut(s) 271
BbvI GCAGC 1 cut(s) 181
BccI CCATC 1 cut(s) 412
BciT130I CCWGG 2 cut(s) 53, 305
BfaI CTAG 1 cut(s) 530
BisI GCNGC 2 cut(s) 85, 195
BlsI GCNGC 2 cut(s) 86, 196
Bme1390I CCNGG 2 cut(s) 53, 305
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 2 cut(s) 55, 307
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 2 cut(s) 53, 305
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 1 cut(s) 366
BmuI ACTGGG 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 127
BpuEI CTTGAG 1 cut(s) 381
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse118I RCCGGY 1 cut(s) 502
Bse1I ACTGG 2 cut(s) 310, 458
Bse3DI GCAATG 2 cut(s) 290, 330
BseBI CCWGG 2 cut(s) 53, 305
BseGI GGATG 2 cut(s) 381, 423
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMI GCAATG 2 cut(s) 290, 330
BseNI ACTGG 2 cut(s) 310, 458
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 1 cut(s) 181
BseYI CCCAGC 2 cut(s) 99, 464
BshFI GGCC 2 cut(s) 215, 308
BshNI GGYRCC 1 cut(s) 271
BsiSI CCGG 1 cut(s) 503
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 458
BsnI GGCC 2 cut(s) 215, 308
Bsp143I GATC 1 cut(s) 34
BspACI CCGC 1 cut(s) 85
BspANI GGCC 2 cut(s) 215, 308
BspHI TCATGA 1 cut(s) 548
BspLI GGNNCC 1 cut(s) 273
BspPI GGATC 1 cut(s) 29
BspT107I GGYRCC 1 cut(s) 271
BsrDI GCAATG 2 cut(s) 290, 330
BsrFI RCCGGY 1 cut(s) 502
BsrI ACTGG 2 cut(s) 310, 458
BssAI RCCGGY 1 cut(s) 502
BssMI GATC 1 cut(s) 34
Bst2UI CCWGG 2 cut(s) 53, 305
Bst4CI ACNGT 3 cut(s) 264, 386, 541
Bst6I CTCTTC 2 cut(s) 143, 404
BstC8I GCNNGC 1 cut(s) 82
BstF5I GGATG 2 cut(s) 381, 423
BstKTI GATC 1 cut(s) 37
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 1 cut(s) 470
BstNI CCWGG 2 cut(s) 53, 305
BstSCI CCNGG 2 cut(s) 51, 303
BstV1I GCAGC 1 cut(s) 181
BsuRI GGCC 2 cut(s) 215, 308
BtsCI GGATG 2 cut(s) 381, 423
BtsIMutI CAGTG 2 cut(s) 246, 363
Cac8I GCNNGC 1 cut(s) 82
CaiI CAGNNNCTG 2 cut(s) 58, 464
CciI TCATGA 1 cut(s) 548
Cfr10I RCCGGY 1 cut(s) 502
Cfr13I GGNCC 2 cut(s) 55, 307
CsiI ACCWGGT 1 cut(s) 51
Csp6I GTAC 2 cut(s) 96, 260
CviAII CATG 1 cut(s) 549
CviQI GTAC 2 cut(s) 96, 260
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 143, 404
EarI CTCTTC 2 cut(s) 143, 404
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 3 cut(s) 80, 281, 444
EcoO109I RGGNCCY 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 51, 303
FaeI CATG 1 cut(s) 552
FaiI YATR 5 cut(s) 15, 179, 471, 476, 550
FaqI GGGAC 1 cut(s) 458
FatI CATG 1 cut(s) 548
Fnu4HI GCNGC 2 cut(s) 85, 195
FokI GGATG 2 cut(s) 388, 430
Fsp4HI GCNGC 2 cut(s) 85, 195
FspBI CTAG 1 cut(s) 530
GluI GCNGC 2 cut(s) 85, 195
GsaI CCCAGC 2 cut(s) 103, 468
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 2 cut(s) 215, 308
HapII CCGG 1 cut(s) 503
Hin1II CATG 1 cut(s) 552
HinfI GANTC 4 cut(s) 116, 368, 412, 545
HpaII CCGG 1 cut(s) 503
Hpy188I TCNGA 1 cut(s) 417
Hpy188III TCNNGA 4 cut(s) 44, 58, 398, 549
HpyAV CCTTC 4 cut(s) 55, 160, 291, 329
HpyCH4III ACNGT 3 cut(s) 264, 386, 541
HpyCH4V TGCA 2 cut(s) 17, 197
HpyF10VI GCNNNNNNNGC 1 cut(s) 470
Hsp92II CATG 1 cut(s) 552
Kzo9I GATC 1 cut(s) 34
LmnI GCTCC 2 cut(s) 152, 237
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 1 cut(s) 366
MabI ACCWGGT 1 cut(s) 51
MaeI CTAG 1 cut(s) 530
MaeIII GTNAC 2 cut(s) 3, 533
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 3 cut(s) 130, 340, 421
MfeI CAATTG 1 cut(s) 231
MluCI AATT 2 cut(s) 231, 345
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 1 cut(s) 395
MnlI CCTC 5 cut(s) 135, 219, 313, 346, 435
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 2 cut(s) 53, 305
MunI CAATTG 1 cut(s) 231
MvaI CCWGG 2 cut(s) 53, 305
MwoI GCNNNNNNNGC 1 cut(s) 470
NdeII GATC 1 cut(s) 34
NlaIII CATG 1 cut(s) 552
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 3
PagI TCATGA 1 cut(s) 548
PfeI GAWTC 3 cut(s) 116, 368, 545
PflMI CCANNNNNTGG 1 cut(s) 464
PkrI GCNGC 2 cut(s) 86, 196
PleI GAGTC 1 cut(s) 420
PpsI GAGTC 1 cut(s) 420
PpuMI RGGWCCY 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 2 cut(s) 51, 303
PspFI CCCAGC 2 cut(s) 99, 464
PspGI CCWGG 2 cut(s) 51, 303
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 2 cut(s) 55, 307
PspPPI RGGWCCY 1 cut(s) 55
PstNI CAGNNNCTG 2 cut(s) 58, 464
RsaI GTAC 2 cut(s) 97, 261
RsaNI GTAC 2 cut(s) 96, 260
SatI GCNGC 2 cut(s) 85, 195
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 2 cut(s) 55, 307
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 2 cut(s) 53, 305
SetI ASST 9 cut(s) 27, 43, 57, 105, 149, 171, 242, 340, 535
SexAI ACCWGGT 1 cut(s) 51
SfaNI GCATC 1 cut(s) 366
SinI GGWCC 1 cut(s) 55
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 2 cut(s) 231, 345
SsiI CCGC 1 cut(s) 85
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 2 cut(s) 51, 303
TaaI ACNGT 3 cut(s) 264, 386, 541
TaqI TCGA 6 cut(s) 21, 33, 108, 114, 156, 171
TasI AATT 2 cut(s) 231, 345
TatI WGTACW 1 cut(s) 259
TauI GCSGC 1 cut(s) 87
TfiI GAWTC 3 cut(s) 116, 368, 545
TscAI CASTG 2 cut(s) 253, 370
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 3 cut(s) 142, 279, 341
TspRI CASTG 2 cut(s) 253, 370
Van91I CCANNNNNTGG 1 cut(s) 464
VpaK11BI GGWCC 1 cut(s) 55
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.