Rh2DG646400
ERF Family

2'-5' RNA ligase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004409
Physical Location & Seq
Reverse (-)
87293115 .. 87295762
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG646400.1

Sequence Viewer

Length: 552 bp
ATGTCACAAGGCTATGCAATCGAGCTTTACTTCGATCCAGCGCTGGAAAACCAGGTCCTGAAGGCTTGGAATGTTCTTGCTCGCCGCCAGATTAGCACCCAGCTCATCGAAATCGAATCACGCCCTCACATCACTCTCTTCTCCAGCTCCTTCATCGAACCAGCAAAGCTCGAAAACATAATCAAGTCTTTCGCTGCAAAGCAAGAGCCTCTGGCCTTGTCTTTCTCCTCAATTGGGAGCTTCCCCACTGATAACAATGTACTGTTTCTGGCACCAACTCCTTCGGTTTCATTGCTTCAGTTCCAGTCCCAGTTGTGTGAGGCAATGAAGAAAGAAGGTGTCGAAATTGGGGAGGAGTATCGGACTGATTCTTGGATGCCTTTCTGTGCTGTTGCTCAAGATGTGCCGAAGAGTCGGATGGCTGAAGCGTTTTGCGTATTGAGGGACTTGAAGTTGCCGGTTGCTGGGTATGCTATGGATATTGGGCTGGTGGAGTTTTCGCCGGTGAGGGAGTTGTTTTCTTTTGTGCTAGGTAACACAGTAGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2_5_RNA_ligase2

Protein Analysis

183

Amino Acids

20.42

Weight (kDa)

4.78

Isoelectric Point (pI)

69.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2_5_RNA_ligase2 PF13563 40 - 163 2.6e-14 2'-5' RNA ligase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 271
AciI CCGC 1 cut(s) 85
AclWI GGATC 1 cut(s) 29
AcuI CTGAAG 3 cut(s) 80, 281, 444
AfaI GTAC 1 cut(s) 261
AfeI AGCGCT 1 cut(s) 42
AfiI CCNNNNNNNGG 2 cut(s) 234, 464
AgsI TTSAA 1 cut(s) 451
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 6 cut(s) 25, 103, 147, 169, 240, 548
AluI AGCT 6 cut(s) 25, 103, 147, 169, 240, 548
AlwI GGATC 1 cut(s) 29
AlwNI CAGNNNCTG 1 cut(s) 58
Aor51HI AGCGCT 1 cut(s) 42
AoxI GGCC 1 cut(s) 213
ApeKI GCWGC 1 cut(s) 194
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 1 cut(s) 55
AsuHPI GGTGA 1 cut(s) 517
AvaII GGWCC 1 cut(s) 55
BanI GGYRCC 1 cut(s) 271
BbvI GCAGC 1 cut(s) 181
BccI CCATC 1 cut(s) 412
BciT130I CCWGG 1 cut(s) 53
BfaI CTAG 1 cut(s) 530
BfoI RGCGCY 1 cut(s) 44
BisI GCNGC 2 cut(s) 85, 195
BlsI GCNGC 2 cut(s) 86, 196
Bme1390I CCNGG 1 cut(s) 53
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 1 cut(s) 53
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 1 cut(s) 366
BmuI ACTGGG 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 127
BpuEI CTTGAG 1 cut(s) 381
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 464
Bse118I RCCGGY 2 cut(s) 457, 502
Bse1I ACTGG 2 cut(s) 304, 310
Bse3DI GCAATG 2 cut(s) 290, 330
BseBI CCWGG 1 cut(s) 53
BseGI GGATG 2 cut(s) 381, 423
BseLI CCNNNNNNNGG 2 cut(s) 234, 464
BseMI GCAATG 2 cut(s) 290, 330
BseNI ACTGG 2 cut(s) 304, 310
BseRI GAGGAG 2 cut(s) 217, 368
BseXI GCAGC 1 cut(s) 181
BseYI CCCAGC 2 cut(s) 99, 464
BshFI GGCC 1 cut(s) 215
BshNI GGYRCC 1 cut(s) 271
BsiSI CCGG 2 cut(s) 458, 503
BslFI GGGAC 2 cut(s) 292, 458
BslI CCNNNNNNNGG 2 cut(s) 234, 464
BsmFI GGGAC 2 cut(s) 292, 458
BsnI GGCC 1 cut(s) 215
Bsp143I GATC 1 cut(s) 34
BspACI CCGC 1 cut(s) 85
BspANI GGCC 1 cut(s) 215
BspLI GGNNCC 1 cut(s) 273
BspPI GGATC 1 cut(s) 29
BspT107I GGYRCC 1 cut(s) 271
BsrDI GCAATG 2 cut(s) 290, 330
BsrFI RCCGGY 2 cut(s) 457, 502
BsrI ACTGG 2 cut(s) 304, 310
BssAI RCCGGY 2 cut(s) 457, 502
BssMI GATC 1 cut(s) 34
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 2 cut(s) 264, 541
Bst6I CTCTTC 2 cut(s) 143, 404
BstC8I GCNNGC 1 cut(s) 82
BstF5I GGATG 2 cut(s) 381, 423
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 1 cut(s) 37
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 2 cut(s) 93, 470
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 51
BstV1I GCAGC 1 cut(s) 181
BsuRI GGCC 1 cut(s) 215
BtsCI GGATG 2 cut(s) 381, 423
BtsIMutI CAGTG 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 82
CaiI CAGNNNCTG 1 cut(s) 58
CfoI GCGC 1 cut(s) 43
Cfr10I RCCGGY 2 cut(s) 457, 502
Cfr13I GGNCC 1 cut(s) 55
CsiI ACCWGGT 1 cut(s) 51
Csp6I GTAC 1 cut(s) 260
CviQI GTAC 1 cut(s) 260
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 143, 404
EarI CTCTTC 2 cut(s) 143, 404
Eco47I GGWCC 1 cut(s) 55
Eco47III AGCGCT 1 cut(s) 42
Eco57I CTGAAG 3 cut(s) 80, 281, 444
EcoO109I RGGNCCY 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 51
FaiI YATR 4 cut(s) 15, 179, 471, 476
FaqI GGGAC 2 cut(s) 292, 458
Fnu4HI GCNGC 2 cut(s) 85, 195
FokI GGATG 2 cut(s) 388, 430
Fsp4HI GCNGC 2 cut(s) 85, 195
FspBI CTAG 1 cut(s) 530
GlaI GCGC 1 cut(s) 42
GluI GCNGC 2 cut(s) 85, 195
GsaI CCCAGC 2 cut(s) 103, 468
GsuI CTGGAG 1 cut(s) 127
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 1 cut(s) 215
HapII CCGG 2 cut(s) 458, 503
HhaI GCGC 1 cut(s) 43
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HindIII AAGCTT 1 cut(s) 546
HinfI GANTC 3 cut(s) 116, 368, 412
HpaII CCGG 2 cut(s) 458, 503
HphI GGTGA 1 cut(s) 517
Hpy188I TCNGA 2 cut(s) 363, 417
Hpy188III TCNNGA 2 cut(s) 58, 398
HpyAV CCTTC 4 cut(s) 55, 160, 291, 329
HpyCH4III ACNGT 2 cut(s) 264, 541
HpyCH4V TGCA 2 cut(s) 17, 197
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 470
HspAI GCGC 1 cut(s) 41
Kzo9I GATC 1 cut(s) 34
LmnI GCTCC 2 cut(s) 152, 237
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 1 cut(s) 366
MabI ACCWGGT 1 cut(s) 51
MaeI CTAG 1 cut(s) 530
MaeIII GTNAC 2 cut(s) 3, 533
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 3 cut(s) 130, 340, 421
MfeI CAATTG 1 cut(s) 231
MluCI AATT 2 cut(s) 231, 345
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 1 cut(s) 395
MnlI CCTC 7 cut(s) 135, 219, 238, 313, 346, 435, 501
MspI CCGG 2 cut(s) 458, 503
MspR9I CCNGG 1 cut(s) 53
MunI CAATTG 1 cut(s) 231
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 93, 470
NdeII GATC 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 3
PfeI GAWTC 2 cut(s) 116, 368
PkrI GCNGC 2 cut(s) 86, 196
PleI GAGTC 1 cut(s) 420
PpsI GAGTC 1 cut(s) 420
PpuMI RGGWCCY 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 51
PspFI CCCAGC 2 cut(s) 99, 464
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 1 cut(s) 55
PspPPI RGGWCCY 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 58
RsaI GTAC 1 cut(s) 261
RsaNI GTAC 1 cut(s) 260
SatI GCNGC 2 cut(s) 85, 195
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 1 cut(s) 55
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 1 cut(s) 53
SetI ASST 9 cut(s) 27, 57, 105, 149, 171, 242, 340, 535, 550
SexAI ACCWGGT 1 cut(s) 51
SfaNI GCATC 1 cut(s) 366
SgrAI CRCCGGYG 1 cut(s) 502
SinI GGWCC 1 cut(s) 55
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 2 cut(s) 231, 345
SsiI CCGC 1 cut(s) 85
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 1 cut(s) 51
TaaI ACNGT 2 cut(s) 264, 541
TaqI TCGA 7 cut(s) 21, 33, 108, 114, 156, 171, 342
TasI AATT 2 cut(s) 231, 345
TatI WGTACW 1 cut(s) 259
TauI GCSGC 1 cut(s) 87
TfiI GAWTC 2 cut(s) 116, 368
TscAI CASTG 1 cut(s) 253
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 3 cut(s) 142, 279, 341
TspRI CASTG 1 cut(s) 253
VpaK11BI GGWCC 1 cut(s) 55
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.