FvH4_6g50340
ERF Family

2'-5' RNA ligase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004409
Physical Location & Seq
Reverse (-)
37487214 .. 37490066
2853 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50340.t1

Sequence Viewer

Length: 615 bp
ATGTCACAAGGCTATGCAATCGAGCTTTACTTCGATCCTGCGCTGGAGAACCAGGTCCTGAAGGCTTGGAATGTTCTTGCTCGCCGCCAGATTAGCACCCAGCTCATCGAAATCGAATCACGCCCTCACATCACTCTCTTCTCCAGCTCCTTCATTGAACCAGCAAAGCTCGAAAACATAATCAAGGCTTTCGCTGCAAAGCAAGAGCCTTTGGCCTTGTCTTTCTCGTCCATTGGGAGCTTTCCGACTGATAACAATGTGCTGTTTCTGGCCCCAACTCCTTCGGTTTCGTTGCTTCAGTTCCAGTCGCAGTTGTGTGAGGCAATGAAGAAAGAAGGTGTTGAAATTGGGGAGGAGTATCGCACCGATTCGTGGATGCCTTACTGTGCTGTTGCTCAAGATGTGCCAAAGAGTCGGATGGCAGAAGCGTTCTGCGTATTGAGGGAATTAAAGTTGCCAGTCGCTGGGTATGCTATGGATGTTGGGCTGGTTGAGTTTTCGCCAGTCAGGGAGTTGTTTTCTTTTGTGCTAGATATCAAGTTAACTACACAAGAAAACAGAACCCAGATATCTGATTTGTGTGCTCGGGTGCTTAGTGTTTTTGTCGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2_5_RNA_ligase2

Protein Analysis

205

Amino Acids

22.94

Weight (kDa)

4.83

Isoelectric Point (pI)

64.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2_5_RNA_ligase2 PF13563 40 - 163 8.5e-15 2'-5' RNA ligase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 464
AciI CCGC 1 cut(s) 85
AclWI GGATC 1 cut(s) 29
AcuI CTGAAG 2 cut(s) 80, 281
AfiI CCNNNNNNNGG 2 cut(s) 372, 464
AgsI TTSAA 2 cut(s) 158, 344
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 5 cut(s) 25, 103, 147, 169, 240
AluI AGCT 5 cut(s) 25, 103, 147, 169, 240
Alw21I GWGCWC 1 cut(s) 586
AlwI GGATC 1 cut(s) 29
AlwNI CAGNNNCTG 2 cut(s) 58, 464
Ama87I CYCGRG 1 cut(s) 585
AoxI GGCC 2 cut(s) 213, 270
ApeKI GCWGC 1 cut(s) 194
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 2 cut(s) 55, 271
AvaI CYCGRG 1 cut(s) 585
AvaII GGWCC 1 cut(s) 55
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 1 cut(s) 181
BccI CCATC 1 cut(s) 412
BciT130I CCWGG 1 cut(s) 53
BfaI CTAG 1 cut(s) 530
BisI GCNGC 2 cut(s) 85, 195
BlsI GCNGC 2 cut(s) 86, 196
Bme1390I CCNGG 1 cut(s) 53
Bme18I GGWCC 1 cut(s) 55
BmeT110I CYCGRG 1 cut(s) 585
BmgT120I GGNCC 2 cut(s) 55, 271
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 1 cut(s) 53
BmsI GCATC 1 cut(s) 366
BpmI CTGGAG 2 cut(s) 65, 127
BpuEI CTTGAG 1 cut(s) 381
Bsc4I CCNNNNNNNGG 2 cut(s) 372, 464
Bse1I ACTGG 3 cut(s) 304, 458, 503
Bse3DI GCAATG 1 cut(s) 330
BseBI CCWGG 1 cut(s) 53
BseGI GGATG 3 cut(s) 381, 423, 484
BseLI CCNNNNNNNGG 2 cut(s) 372, 464
BseMI GCAATG 1 cut(s) 330
BseNI ACTGG 3 cut(s) 304, 458, 503
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 1 cut(s) 181
BseYI CCCAGC 2 cut(s) 99, 464
BshFI GGCC 2 cut(s) 215, 272
BsiHKAI GWGCWC 1 cut(s) 586
BsiHKCI CYCGRG 1 cut(s) 585
BslI CCNNNNNNNGG 2 cut(s) 372, 464
BsnI GGCC 2 cut(s) 215, 272
BsoBI CYCGRG 1 cut(s) 585
Bsp1286I GDGCHC 1 cut(s) 586
Bsp143I GATC 1 cut(s) 34
BspACI CCGC 1 cut(s) 85
BspANI GGCC 2 cut(s) 215, 272
BspLI GGNNCC 1 cut(s) 273
BspPI GGATC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 330
BsrI ACTGG 3 cut(s) 304, 458, 503
BssMI GATC 1 cut(s) 34
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 386
Bst6I CTCTTC 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 82
BstDEI CTNAG 1 cut(s) 593
BstF5I GGATG 3 cut(s) 381, 423, 484
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 1 cut(s) 37
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 3 cut(s) 93, 194, 470
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 51
BstV1I GCAGC 1 cut(s) 181
BsuRI GGCC 2 cut(s) 215, 272
BtsCI GGATG 3 cut(s) 381, 423, 484
Cac8I GCNNGC 1 cut(s) 82
CaiI CAGNNNCTG 2 cut(s) 58, 464
CfoI GCGC 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 55, 271
CsiI ACCWGGT 1 cut(s) 51
DdeI CTNAG 1 cut(s) 593
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco32I GATATC 2 cut(s) 535, 570
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 2 cut(s) 80, 281
Eco88I CYCGRG 1 cut(s) 585
EcoO109I RGGNCCY 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 51
EcoRV GATATC 2 cut(s) 535, 570
FaiI YATR 4 cut(s) 15, 179, 471, 476
Fnu4HI GCNGC 2 cut(s) 85, 195
FokI GGATG 3 cut(s) 388, 430, 491
Fsp4HI GCNGC 2 cut(s) 85, 195
FspBI CTAG 1 cut(s) 530
GlaI GCGC 1 cut(s) 42
GluI GCNGC 2 cut(s) 85, 195
GsaI CCCAGC 2 cut(s) 103, 468
GsuI CTGGAG 2 cut(s) 65, 127
HaeIII GGCC 2 cut(s) 215, 272
HhaI GCGC 1 cut(s) 43
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HincII GTYRAC 1 cut(s) 543
HindII GTYRAC 1 cut(s) 543
HinfI GANTC 3 cut(s) 116, 368, 412
HpaI GTTAAC 1 cut(s) 543
Hpy166II GTNNAC 1 cut(s) 543
Hpy188I TCNGA 3 cut(s) 246, 417, 574
Hpy188III TCNNGA 2 cut(s) 58, 398
Hpy8I GTNNAC 1 cut(s) 543
HpyAV CCTTC 4 cut(s) 55, 160, 291, 329
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4V TGCA 2 cut(s) 17, 197
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 194, 470
HpyF3I CTNAG 1 cut(s) 593
HspAI GCGC 1 cut(s) 41
KspAI GTTAAC 1 cut(s) 543
Kzo9I GATC 1 cut(s) 34
LmnI GCTCC 2 cut(s) 152, 237
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 1 cut(s) 366
MabI ACCWGGT 1 cut(s) 51
MaeI CTAG 1 cut(s) 530
MaeIII GTNAC 1 cut(s) 3
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 2 cut(s) 130, 340
MhlI GDGCHC 1 cut(s) 586
MluCI AATT 2 cut(s) 345, 446
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 2 cut(s) 269, 395
MnlI CCTC 5 cut(s) 135, 313, 346, 435, 601
MseI TTAA 2 cut(s) 449, 542
MspR9I CCNGG 1 cut(s) 53
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 3 cut(s) 93, 194, 470
NdeII GATC 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 3
PfeI GAWTC 2 cut(s) 116, 368
PflMI CCANNNNNTGG 1 cut(s) 464
PkrI GCNGC 2 cut(s) 86, 196
PleI GAGTC 1 cut(s) 420
PpsI GAGTC 1 cut(s) 420
PpuMI RGGWCCY 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 51
PspFI CCCAGC 2 cut(s) 99, 464
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 2 cut(s) 55, 271
PspPPI RGGWCCY 1 cut(s) 55
PstNI CAGNNNCTG 2 cut(s) 58, 464
SaqAI TTAA 2 cut(s) 449, 542
SatI GCNGC 2 cut(s) 85, 195
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 2 cut(s) 55, 271
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 1 cut(s) 53
SduI GDGCHC 1 cut(s) 586
SetI ASST 7 cut(s) 27, 57, 105, 149, 171, 242, 340
SexAI ACCWGGT 1 cut(s) 51
SfaNI GCATC 1 cut(s) 366
SinI GGWCC 1 cut(s) 55
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 2 cut(s) 345, 446
SsiI CCGC 1 cut(s) 85
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 386
TaqI TCGA 6 cut(s) 21, 33, 108, 114, 171, 606
TasI AATT 2 cut(s) 345, 446
TauI GCSGC 1 cut(s) 87
TfiI GAWTC 2 cut(s) 116, 368
Tru1I TTAA 2 cut(s) 449, 542
Tru9I TTAA 2 cut(s) 449, 542
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 2 cut(s) 142, 341
Van91I CCANNNNNTGG 1 cut(s) 464
VpaK11BI GGWCC 1 cut(s) 55
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.