AT5G40310

Exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011689
Physical Location & Seq
Reverse (-)
16113079 .. 16116171
3093 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G40310.1

Sequence Viewer

Length: 1047 bp
ATGGATACATGCAGGAACAAGTGTGGAGGGTGTTATAGGCAATTCAACAAGAAGGAGCACTTGGTGGAACACATGAGGATCTCTTATCATTCGGTTCATGAACCTACTTGTGGTATTTGCAACAAACATTGCCGATCTTTTGACTCCCTCCGTGAACATCTCATTGGGCCATTGCCGAAACAAGAATGTAAGAACATTTTCAGCATTCGCGGCTGCAGATTCTGTCTTACGATCCTCGAAAGCCCCAACGCTCGTAGAATCCATCAAGAGAGATGCCAGCTCTCAAACGTCACTTCTGGATTAATGATTCGTATGGCGGCCTTAGGCCTAAGAAACAACTCAACAATTGACTACACTTCTTCGAGGTCACCTCGAGTGGTGGCACTCTCATGCAAGATGGTTGGAGGAGGCAGTGACGGATCGCTTGACCTATGCGCAAGAGTTTGCATTACGGATGAGAGCGAAAATGTTGTGTTCCACACGTATGTGAAGCCAACGATACCCGTAACGAATTATAGGTATGAGATGACAGGGATTCGACCTGAAAATCTAAGGGACGCAATGCGATTAAAGCACGCACAGAGAAAGGTTCAAGAGTTTCTTTGTAATGGAGAACCAATGTGGAAGATTCGTCCAAGAAATGGGAAAGCAAGGATTCTCGTTGGACATGGACTTGATAACCATCTTGACTCTCTTCAACTTGAATATTCTTCCTCTATGATAAGAGATACTGCGGAATACCCTCCATTGATGAAATCAAGCAAGCTAAGCAACTCTCTCAAGTACTTAACCCAAGCCTATCTCGGTTATGATATTCATGTGGGAATACAAGATCCTTACGAGGACTGTGTCGCGACAATGAGGCTATACACGAGAATGCGATATCAGAAACACAGGGCCGAGGCCTATCCGCTGGCCTCGGACACGCAGAACCACAATAACTTTGCGGCGTGGAGGCAGAATGAACTAGAGAGGATGTCTCCAGAGGAGTTGCTCGACCTTTCACGTTCAGACTATTACTGCTGGTGCTTGGACTCGGTTGCTTGA

Protein Analysis

348

Amino Acids

40.1

Weight (kDa)

8.76

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 143 - 268 1.6e-07 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011689)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27970 AT5G40310 AT5G40310
fragaria_vesca FvH4_6g50710
malus_domestica MD00G1089900.v1.1 MD00G1186700.v1.1
prunus_persica Prupe.3G285000_v2.0.a1 Prupe.3G285000_v2.0.a1
pyrus_communis pycom11g21450
rosa_chinensis RchiOBHm_Chr2g0171511
rosa_laevigata RLG00000022021
rosa_roxburghii Rroxscaffold_2G00080580
rosa_rugosa Rorug02G0554800
rosa_samantha Rh2AG629200 Rh2BG639800 Rh2CG608300 Rh2DG650600 Rh2DG656300
rosa_wichuraiana Rw2G052120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 436
AccB7I CCANNNNNTGG 1 cut(s) 641
AccII CGCG 2 cut(s) 210, 854
AciI CCGC 5 cut(s) 210, 317, 734, 911, 947
AclWI GGATC 4 cut(s) 86, 226, 427, 827
AdeI CACNNNGTG 1 cut(s) 64
AfaI GTAC 1 cut(s) 785
AfiI CCNNNNNNNGG 2 cut(s) 110, 641
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 4 cut(s) 46, 593, 698, 704
AjuI GAANNNNNNNTTGG 4 cut(s) 44, 76, 147, 179
AleI CACNNNNGTG 1 cut(s) 485
AluBI AGCT 2 cut(s) 280, 766
AluI AGCT 2 cut(s) 280, 766
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 1 cut(s) 984
AlwI GGATC 4 cut(s) 86, 226, 427, 827
AlwNI CAGNNNCTG 1 cut(s) 222
Ama87I CYCGRG 1 cut(s) 372
AoxI GGCC 6 cut(s) 167, 318, 325, 897, 903, 915
ApeKI GCWGC 1 cut(s) 213
AseI ATTAAT 1 cut(s) 302
Asp700I GAANNNNTTC 1 cut(s) 197
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 2 cut(s) 167, 897
AsuHPI GGTGA 1 cut(s) 360
AvaI CYCGRG 1 cut(s) 372
AxyI CCTNAGG 1 cut(s) 322
BauI CACGAG 1 cut(s) 871
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 200
BccI CCATC 3 cut(s) 270, 391, 690
BcoDI GTCTC 1 cut(s) 984
BfaI CTAG 1 cut(s) 968
BfmI CTRYAG 1 cut(s) 214
BisI GCNGC 4 cut(s) 211, 214, 318, 948
BlpI GCTNAGC 1 cut(s) 767
BlsI GCNGC 4 cut(s) 212, 215, 319, 949
BmcAI AGTACT 1 cut(s) 785
BmeT110I CYCGRG 1 cut(s) 372
BmgT120I GGNCC 2 cut(s) 167, 897
BmsI GCATC 1 cut(s) 263
BplI GAGNNNNNCTC 4 cut(s) 355, 387, 964, 996
BpmI CTGGAG 1 cut(s) 966
Bpu1102I GCTNAGC 1 cut(s) 767
BpuEI CTTGAG 1 cut(s) 764
BsaAI YACGTR 1 cut(s) 483
BsaBI GATNNNNATC 1 cut(s) 681
BsaJI CCNNGG 2 cut(s) 900, 918
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 641
Bse21I CCTNAGG 1 cut(s) 322
Bse3DI GCAATG 3 cut(s) 127, 170, 567
Bse8I GATNNNNATC 1 cut(s) 681
BseDI CCNNGG 2 cut(s) 900, 918
BseGI GGATG 2 cut(s) 460, 981
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 2 cut(s) 110, 641
BseMI GCAATG 3 cut(s) 127, 170, 567
BseRI GAGGAG 2 cut(s) 420, 1001
BseXI GCAGC 1 cut(s) 200
Bsh1236I CGCG 2 cut(s) 210, 854
BshFI GGCC 6 cut(s) 169, 320, 327, 899, 905, 917
BsiHKAI GWGCWC 1 cut(s) 60
BsiHKCI CYCGRG 1 cut(s) 372
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 2 cut(s) 110, 641
BsmAI GTCTC 1 cut(s) 984
BsmFI GGGAC 1 cut(s) 569
BsmI GAATGC 2 cut(s) 204, 882
BsnI GGCC 6 cut(s) 169, 320, 327, 899, 905, 917
BsoBI CYCGRG 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 5 cut(s) 78, 134, 231, 419, 832
Bsp1720I GCTNAGC 1 cut(s) 767
Bsp68I TCGCGA 1 cut(s) 854
BspACI CCGC 5 cut(s) 210, 317, 734, 911, 947
BspANI GGCC 6 cut(s) 169, 320, 327, 899, 905, 917
BspFNI CGCG 2 cut(s) 210, 854
BspHI TCATGA 1 cut(s) 97
BspMAI CTGCAG 1 cut(s) 218
BspPI GGATC 4 cut(s) 86, 226, 427, 827
BsrDI GCAATG 3 cut(s) 127, 170, 567
BssECI CCNNGG 2 cut(s) 900, 918
BssMI GATC 5 cut(s) 78, 134, 231, 419, 832
BssSI CACGAG 1 cut(s) 871
Bst2BI CACGAG 1 cut(s) 871
Bst4CI ACNGT 1 cut(s) 848
Bst6I CTCTTC 1 cut(s) 699
BstBAI YACGTR 1 cut(s) 483
BstC8I GCNNGC 4 cut(s) 278, 576, 764, 915
BstDEI CTNAG 4 cut(s) 322, 329, 551, 767
BstEII GGTNACC 1 cut(s) 366
BstF5I GGATG 2 cut(s) 460, 981
BstFNI CGCG 2 cut(s) 210, 854
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 5 cut(s) 81, 137, 234, 422, 835
BstMAI GTCTC 1 cut(s) 984
BstMBI GATC 5 cut(s) 78, 134, 231, 419, 832
BstMWI GCNNNNNNNGC 3 cut(s) 210, 571, 768
BstNSI RCATGY 1 cut(s) 12
BstPI GGTNACC 1 cut(s) 366
BstSFI CTRYAG 1 cut(s) 214
BstUI CGCG 2 cut(s) 210, 854
BstV1I GCAGC 1 cut(s) 200
BstX2I RGATCY 2 cut(s) 78, 832
BstYI RGATCY 2 cut(s) 78, 832
Bsu36I CCTNAGG 1 cut(s) 322
BsuRI GGCC 6 cut(s) 169, 320, 327, 899, 905, 917
BtsCI GGATG 2 cut(s) 460, 981
BtsI GCAGTG 1 cut(s) 418
BtsIMutI CAGTG 1 cut(s) 418
BtuMI TCGCGA 1 cut(s) 854
Cac8I GCNNGC 4 cut(s) 278, 576, 764, 915
CaiI CAGNNNCTG 1 cut(s) 222
CciI TCATGA 1 cut(s) 97
CfoI GCGC 1 cut(s) 437
Cfr13I GGNCC 2 cut(s) 167, 897
CseI GACGC 1 cut(s) 566
Csp6I GTAC 1 cut(s) 784
CviAII CATG 6 cut(s) 9, 73, 98, 390, 668, 818
CviQI GTAC 1 cut(s) 784
DdeI CTNAG 4 cut(s) 322, 329, 551, 767
DpnI GATC 5 cut(s) 80, 136, 233, 421, 834
DpnII GATC 5 cut(s) 78, 134, 231, 419, 832
DraIII CACNNNGTG 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 699
EarI CTCTTC 1 cut(s) 699
Eco147I AGGCCT 2 cut(s) 327, 905
Eco32I GATATC 1 cut(s) 884
Eco81I CCTNAGG 1 cut(s) 322
Eco88I CYCGRG 1 cut(s) 372
Eco91I GGTNACC 1 cut(s) 366
EcoO65I GGTNACC 1 cut(s) 366
EcoRV GATATC 1 cut(s) 884
FaeI CATG 6 cut(s) 12, 76, 101, 393, 671, 821
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 585, 617
FaqI GGGAC 1 cut(s) 569
FatI CATG 6 cut(s) 8, 72, 97, 389, 667, 817
Fnu4HI GCNGC 4 cut(s) 211, 214, 318, 948
FokI GGATG 2 cut(s) 467, 988
Fsp4HI GCNGC 4 cut(s) 211, 214, 318, 948
FspBI CTAG 1 cut(s) 968
FspI TGCGCA 1 cut(s) 436
GlaI GCGC 1 cut(s) 436
GluI GCNGC 4 cut(s) 211, 214, 318, 948
GsuI CTGGAG 1 cut(s) 966
HaeIII GGCC 6 cut(s) 169, 320, 327, 899, 905, 917
HgaI GACGC 1 cut(s) 566
HhaI GCGC 1 cut(s) 437
Hin1II CATG 6 cut(s) 12, 76, 101, 393, 671, 821
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HinfI GANTC 9 cut(s) 143, 219, 258, 307, 535, 628, 655, 689, 1034
HphI GGTGA 1 cut(s) 360
Hpy166II GTNNAC 1 cut(s) 155
Hpy188I TCNGA 3 cut(s) 888, 922, 1012
Hpy188III TCNNGA 7 cut(s) 98, 266, 297, 593, 686, 853, 983
Hpy8I GTNNAC 1 cut(s) 155
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 1 cut(s) 848
HpyCH4IV ACGT 3 cut(s) 288, 482, 1006
HpyCH4V TGCA 5 cut(s) 12, 120, 216, 393, 447
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 571, 768
HpyF3I CTNAG 4 cut(s) 322, 329, 551, 767
HpySE526I ACGT 3 cut(s) 288, 482, 1006
Hsp92II CATG 6 cut(s) 12, 76, 101, 393, 671, 821
HspAI GCGC 1 cut(s) 435
Kzo9I GATC 5 cut(s) 78, 134, 231, 419, 832
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 8 cut(s) 282, 290, 516, 555, 880, 899, 996, 1009
Lsp1109I GCAGC 1 cut(s) 200
LweI GCATC 1 cut(s) 263
MaeI CTAG 1 cut(s) 968
MaeII ACGT 3 cut(s) 288, 482, 1006
MaeIII GTNAC 4 cut(s) 289, 366, 413, 505
MalI GATC 5 cut(s) 80, 136, 233, 421, 834
MboI GATC 5 cut(s) 78, 134, 231, 419, 832
MboII GAAGA 4 cut(s) 351, 637, 686, 702
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 2 cut(s) 78, 832
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 3 cut(s) 41, 345, 511
MlyI GAGTC 3 cut(s) 137, 683, 1028
MmeI TCCRAC 2 cut(s) 382, 643
MroXI GAANNNNTTC 1 cut(s) 197
MseI TTAA 3 cut(s) 302, 569, 788
MslI CAYNNNNRTG 4 cut(s) 388, 483, 485, 875
MspA1I CMGCKG 1 cut(s) 913
MunI CAATTG 1 cut(s) 345
Mva1269I GAATGC 2 cut(s) 204, 882
MvnI CGCG 2 cut(s) 210, 854
MwoI GCNNNNNNNGC 3 cut(s) 210, 571, 768
NdeII GATC 5 cut(s) 78, 134, 231, 419, 832
NlaIII CATG 6 cut(s) 12, 76, 101, 393, 671, 821
NmeAIII GCCGAG 1 cut(s) 925
NmuCI GTSAC 3 cut(s) 289, 366, 413
NruI TCGCGA 1 cut(s) 854
NsbI TGCGCA 1 cut(s) 436
NspI RCATGY 1 cut(s) 12
OliI CACNNNNGTG 1 cut(s) 485
PaeR7I CTCGAG 1 cut(s) 372
PagI TCATGA 1 cut(s) 97
PceI AGGCCT 2 cut(s) 327, 905
PctI GAATGC 2 cut(s) 204, 882
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 6 cut(s) 219, 258, 307, 535, 628, 655
PflFI GACNNNGTC 1 cut(s) 848
PflMI CCANNNNNTGG 1 cut(s) 641
PkrI GCNGC 4 cut(s) 212, 215, 319, 949
PleI GAGTC 3 cut(s) 137, 683, 1028
PpsI GAGTC 3 cut(s) 137, 683, 1028
Ppu21I YACGTR 1 cut(s) 483
PshBI ATTAAT 1 cut(s) 302
PspEI GGTNACC 1 cut(s) 366
PspPI GGNCC 2 cut(s) 167, 897
PspXI VCTCGAGB 1 cut(s) 372
PstI CTGCAG 1 cut(s) 218
PstNI CAGNNNCTG 1 cut(s) 222
PsuI RGATCY 2 cut(s) 78, 832
PsyI GACNNNGTC 1 cut(s) 848
RruI TCGCGA 1 cut(s) 854
RsaI GTAC 1 cut(s) 785
RsaNI GTAC 1 cut(s) 784
RseI CAYNNNNRTG 4 cut(s) 388, 483, 485, 875
SaqAI TTAA 3 cut(s) 302, 569, 788
SatI GCNGC 4 cut(s) 211, 214, 318, 948
Sau3AI GATC 5 cut(s) 78, 134, 231, 419, 832
Sau96I GGNCC 2 cut(s) 167, 897
ScaI AGTACT 1 cut(s) 785
SchI GAGTC 3 cut(s) 137, 683, 1028
SduI GDGCHC 1 cut(s) 60
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 1 cut(s) 214
Sfr274I CTCGAG 1 cut(s) 372
SlaI CTCGAG 1 cut(s) 372
SmiMI CAYNNNNRTG 4 cut(s) 388, 483, 485, 875
SmlI CTYRAG 2 cut(s) 372, 779
SmoI CTYRAG 2 cut(s) 372, 779
Sse9I AATT 3 cut(s) 41, 345, 511
SseBI AGGCCT 2 cut(s) 327, 905
SsiI CCGC 5 cut(s) 210, 317, 734, 911, 947
SspI AATATT 1 cut(s) 707
SspMI CTAG 1 cut(s) 968
StuI AGGCCT 2 cut(s) 327, 905
TaaI ACNGT 1 cut(s) 848
TaiI ACGT 3 cut(s) 291, 485, 1009
TaqI TCGA 5 cut(s) 237, 362, 373, 538, 996
TasI AATT 3 cut(s) 41, 345, 511
TatI WGTACW 1 cut(s) 783
TauI GCSGC 3 cut(s) 213, 320, 950
TfiI GAWTC 6 cut(s) 219, 258, 307, 535, 628, 655
Tru1I TTAA 3 cut(s) 302, 569, 788
Tru9I TTAA 3 cut(s) 302, 569, 788
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 3 cut(s) 289, 366, 413
TseI GCWGC 1 cut(s) 213
Tsp45I GTSAC 3 cut(s) 289, 366, 413
TspDTI ATGAA 5 cut(s) 86, 114, 767, 806, 978
TspGWI ACGGA 3 cut(s) 140, 432, 467
TspRI CASTG 1 cut(s) 418
Tth111I GACNNNGTC 1 cut(s) 848
Van91I CCANNNNNTGG 1 cut(s) 641
VspI ATTAAT 1 cut(s) 302
XceI RCATGY 1 cut(s) 12
XhoI CTCGAG 1 cut(s) 372
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 1 cut(s) 968
ZrmI AGTACT 1 cut(s) 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.