AT5G42220

Ubiquitin-2 like Rad60 SUMO-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005387
Physical Location & Seq
Forward (+)
16872044 .. 16878383
6340 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G42220.1

Sequence Viewer

Length: 2640 bp
ATGGAAGATCAACCCATTAACCAGTGCTCTAGTAGCACCAATGCGTCGGAGAAAACTCCAGAATCAACCCTTGAGCTTAACATCAAAACGTTAGACTCGCGGACGTATACTTTCCAAGTGAACAAGAATGAAACTGTTTTGCTATTCAAGGAGAAGATTGCTAGTGAGACAGGGGTGCCGGTTGGTCAGCAGCGGCTCATTTTCCGAGGACGGGTTTTGAAAGATGATCATCCTCTCTCGGAGTATCATTTGGAAAATGGGCATACTTTACACTTGATTGTGAGGCAGCCAGCAGAATCAGCTCCTTCCTCTGGTACACCTTCACAAGGGGCAACTGCAAATGATGGAAATAATACAAATGGTGGACCATCTCGAAATGGAAGACATGTCTCCCACAGCGTAGTTCTTGGGTCTTTTAATGTTGGAGATCAGACAGAAGGCATTGTTCCAGACCTCAGCCGGGTCATTGGAGCAGTTCTAAATTCTTTTGGAGTTAGTGGACAGCTTCCTACAAATCACAGTACTAATGGCACGCAATCCTCTATGCCCTCGAATCAGTCGTCTAATGCACCTCCTGGAAATACATCTGATGGAGAGCCTGGCATTGGTGGTCAAAGCCAAGCTACGGGTCATTCACAACCTAGGCAAGCATTTCCTGGTGTATCATTTCAAACATCGATGCCTCGTGTAGTTCAGATTCCTGTTACAGCAGCCACAACGATTCCTATCCCTTCATTCTTAACGCCTATTCCGGATTCCTTAGACACTCTAATGGAGTTCATTAATCGGATGGAGCAGGCATTATCACAAAATGGCTATCAGCCAGATACCTCTTCTGCTGGATCCGGAGGGCGACCAAGGGAAGAGTTGCCTAGAAATAGACGAGGTGCAGCAACACCTGAAGCACTCTCTGTTGTCCTAAGGAATGCACAACATTTACTGAGTGGTCTCGGTGTCTCTTCTTTATCGCATATTGCGGGACGTCTGGAGCAAGATGGTTCATCTTCAGATCCTACTCTAAGGTCACAAATCCAGACAGAGGCCGTGCAAGTAGGTCTTGCCATGCAACATTTAGGGGCCCTCCTACTGGAGCTTGGACGCACAATTTTGACATTGAGAATGGCACCGTCGCCGGAATTATCCTATGTGAATGCAGGGCCTGCTGTGTATATATCTCCATCAGGACCGAATCCTATTATGGTTCAGCCTTTTCCTCACCAAATCAGCCCTCTCTTTACTGGTGCTACTGTCTCATCAAATCCACTAACCGGACCTGTTGGTTTAGGAACTGCCCAAAGGCACATTAATATTCACATACATGCTGGCACATCAGGTTCACCTATGTTGTCATCAGTTGGGAACCAGCGAAGCAATGGAGAAGGAGGACAAGGAGACCGTGACAGCAATACAAGTTCAGTTCCTGCGGCTGTTCCATCACACTCTACCGGTGAGAACGTTTCTGCTGGGGTTCAACCTGGTTTGGGTGATGATGTTTCAGTTGCTCAAATCAATGCAAGGATAAGAGATATGGTAAACATTATGCAAGGAAGAGACCAGATTCCATCGGGAATAGAATCTCTCGAGAGGGATATGTCTACGGGCCATGGTGTTGCCACTGCTATGCCTGAGCAGCCTACCAACATTGCAACTACTTGCGCACCTGAGTCGTCTAGTGGCTCACTGCATGACTTACCATCTGAGAGGAGCAACTCAGTGTGTCAAAATGAAAAAGATCTTGGTGGAGATTTAGAGCATCCAGCCCGAGCAAAAGATACCTCTTGTACTACAGGACAATCATCAGCTCCGTCTGGAGATGCTACCGGTGATGCCAAAGAAACCAATAAGGCTACTCCAGAGGTAGCTACAGCTACACCACTTGGATTGGGGCTTGGCGGTTTAGACCGTAAGAAACGAAGCAAGCAGCCGAAGGTATCAGGTAAGACTGAAGATAGCGGGACTTCAGCTACACTTGAAGGTGTGCAACAAAGCAGTGGGACCAGTGGTCAGCAGCTTTTGCAATCTCTCTTTTCTGGTAGTTCTCGTTCAGATGAGACTGGTCTTAGACGCGGGCAAGGTTCAGATGATCGTGTAGATGTCTCAAGTGCAATGTCTCAGGTCCTGGAGAGCCCTGTATTGGATGGATTGCTAGCAGGGGTATCTCGACAAGCTGGTGTTGATTCACCAAATATGCTGAGAAATATGCTCCAGCAGTTTACCCAGAATCCACAGATCATGAACACAGTCCAACAGATTGCGCAACAGGTGGATGGGCAAGAGATAGAAAATATGATGTCAGGTGGAGCTCAAGGCGAGGGCGGCGGCTTTGATTTCTCTAGAATGGTCCAACAAATGATGCCTCTAGTCTCACGTGCTTTTAGCCAAGGAGGGCCATTGCCTCATCCCGCAACAATACAGCCTGATGATCGTCAACCTTCTCAGGTAAATGTTCAATCCATGGCTCAAATGATTGAGCACTCTGATCCACCAGAGGATGTCTTCCGTGCAATGGTCGAAAATGCTGCGATAAGCCAAGACGAACTAGTGAACGAGCTTTGCTGTGATGAAGCTCTTTCTCAAGAATATGCGGAGTTGCTAAGACGTGATATCGAAGGCCGGCTAAAAGATGATCAAGGTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001822 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010605 GO:0010941 GO:0012501 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031593 GO:0031647 GO:0032268 GO:0032269 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042981 GO:0043021 GO:0043022 GO:0043067 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045861 GO:0045995 GO:0048232 GO:0048285 GO:0048513 GO:0048519 GO:0048523 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070059 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1903046 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

879

Amino Acids

92.55

Weight (kDa)

5.07

Isoelectric Point (pI)

55.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 25 - 94 5e-08 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 26 - 96 2.3e-25 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 985
Acc16I TGCGCA 2 cut(s) 1657, 2256
AccB1I GGYRCC 2 cut(s) 175, 1123
AccI GTMKAC 2 cut(s) 107, 1595
AccII CGCG 2 cut(s) 100, 2067
AccIII TCCGGA 2 cut(s) 751, 845
AclI AACGTT 2 cut(s) 89, 1455
AclWI GGATC 4 cut(s) 837, 850, 1004, 2474
AcsI RAATTY 1 cut(s) 481
AcuI CTGAAG 4 cut(s) 921, 990, 1944, 1965
AcvI CACGTG 1 cut(s) 2369
AcyI GRCGYC 1 cut(s) 982
AfaI GTAC 3 cut(s) 316, 523, 1783
AflIII ACRYGT 1 cut(s) 385
AgeI ACCGGT 2 cut(s) 1445, 1820
AgsI TTSAA 6 cut(s) 148, 220, 671, 1472, 1973, 2450
AhdI GACNNNNNGTC 1 cut(s) 2001
AhlI ACTAGT 1 cut(s) 2539
AjiI CACGTC 1 cut(s) 2600
AjnI CCWGG 5 cut(s) 574, 598, 655, 1474, 2118
AjuI GAANNNNNNNTTGG 2 cut(s) 1719, 1751
Alw21I GWGCWC 3 cut(s) 29, 2305, 2475
AlwI GGATC 4 cut(s) 837, 850, 1004, 2474
AlwNI CAGNNNCTG 3 cut(s) 1160, 1421, 2119
Ama87I CYCGRG 2 cut(s) 1580, 1761
Aor13HI TCCGGA 2 cut(s) 751, 845
AoxI GGCC 6 cut(s) 1041, 1077, 1157, 1600, 2387, 2611
ApaI GGGCCC 1 cut(s) 1081
ApeKI GCWGC 8 cut(s) 190, 286, 710, 890, 1630, 1921, 2008, 2519
ApoI RAATTY 1 cut(s) 481
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
AseI ATTAAT 2 cut(s) 783, 1305
AsiGI ACCGGT 2 cut(s) 1445, 1820
AspA2I CCTAGG 1 cut(s) 641
AspLEI GCGC 2 cut(s) 1658, 2257
AsuC2I CCSGG 1 cut(s) 461
AsuHPI GGTGA 6 cut(s) 1208, 1329, 1460, 1496, 1835, 2172
AsuNHI GCTAGC 1 cut(s) 2146
AvaI CYCGRG 2 cut(s) 1580, 1761
AvaII GGWCC 6 cut(s) 365, 1184, 1271, 1995, 2116, 2341
AvrII CCTAGG 1 cut(s) 641
AxyI CCTNAGG 1 cut(s) 920
BaeGI GKGCMC 1 cut(s) 1081
BamHI GGATCC 1 cut(s) 842
BanI GGYRCC 2 cut(s) 175, 1123
BanII GRGCYC 3 cut(s) 1081, 2129, 2305
BauI CACGAG 1 cut(s) 684
BbrPI CACGTG 1 cut(s) 2369
BbsI GAAGAC 2 cut(s) 388, 2488
Bbv12I GWGCWC 3 cut(s) 29, 2305, 2475
BbvCI CCTCAGC 1 cut(s) 455
BbvI GCAGC 8 cut(s) 202, 298, 722, 902, 1642, 1933, 2020, 2506
BceAI ACGGC 1 cut(s) 1028
BcgI CGANNNNNNTGC 4 cut(s) 1647, 1681, 2501, 2535
BciT130I CCWGG 5 cut(s) 576, 600, 657, 1476, 2120
BclI TGATCA 2 cut(s) 226, 2626
BcnI CCSGG 1 cut(s) 461
BcuI ACTAGT 1 cut(s) 2539
BfaI CTAG 9 cut(s) 30, 162, 642, 873, 1671, 2147, 2334, 2360, 2540
BfmI CTRYAG 2 cut(s) 1785, 1863
BglII AGATCT 1 cut(s) 1732
BlnI CCTAGG 1 cut(s) 641
BmcAI AGTACT 1 cut(s) 523
Bme1390I CCNGG 6 cut(s) 461, 576, 600, 657, 1476, 2120
Bme18I GGWCC 6 cut(s) 365, 1184, 1271, 1995, 2116, 2341
BmeRI GACNNNNNGTC 1 cut(s) 2001
BmeT110I CYCGRG 2 cut(s) 1580, 1761
BmgBI CACGTC 1 cut(s) 2600
BmiI GGNNCC 7 cut(s) 177, 844, 1078, 1079, 1125, 1361, 1996
BmrFI CCNGG 6 cut(s) 461, 576, 600, 657, 1476, 2120
BmsI GCATC 5 cut(s) 669, 1762, 1804, 1816, 2343
BmtI GCTAGC 1 cut(s) 2150
BpiI GAAGAC 2 cut(s) 388, 2488
BpmI CTGGAG 7 cut(s) 42, 1007, 1109, 1830, 1836, 2141, 2189
Bpu10I CCTNAGC 2 cut(s) 455, 1626
BpuEI CTTGAG 4 cut(s) 92, 2083, 2289, 2559
BpuMI CCSGG 1 cut(s) 461
Bsa29I ATCGAT 1 cut(s) 677
BsaAI YACGTR 1 cut(s) 2369
BsaBI GATNNNNATC 2 cut(s) 228, 725
BsaHI GRCGYC 1 cut(s) 982
BsaI GGTCTC 3 cut(s) 953, 1386, 1545
BsaJI CCNNGG 6 cut(s) 205, 641, 857, 1603, 2380, 2454
BsaWI WCCGGW 5 cut(s) 751, 845, 1268, 1445, 1820
Bse118I RCCGGY 4 cut(s) 178, 1445, 1820, 2613
Bse1I ACTGG 5 cut(s) 22, 1092, 1243, 1998, 2059
Bse21I CCTNAGG 1 cut(s) 920
Bse3DI GCAATG 5 cut(s) 1378, 1641, 2112, 2390, 2511
Bse8I GATNNNNATC 2 cut(s) 228, 725
BseAI TCCGGA 2 cut(s) 751, 845
BseBI CCWGG 5 cut(s) 576, 600, 657, 1476, 2120
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 6 cut(s) 205, 641, 857, 1603, 2380, 2454
BseGI GGATG 7 cut(s) 229, 795, 1753, 2143, 2272, 2398, 2497
BseJI GATNNNNATC 2 cut(s) 228, 725
BseMI GCAATG 5 cut(s) 1378, 1641, 2112, 2390, 2511
BseMII CTCAG 9 cut(s) 469, 932, 1617, 1653, 1689, 1725, 2126, 2183, 2450
BseNI ACTGG 5 cut(s) 22, 1092, 1243, 1998, 2059
BseRI GAGGAG 1 cut(s) 1717
BseSI GKGCMC 1 cut(s) 1081
BseXI GCAGC 8 cut(s) 202, 298, 722, 902, 1642, 1933, 2020, 2506
BseYI CCCAGC 1 cut(s) 1463
BsgI GTGCAG 1 cut(s) 909
Bsh1236I CGCG 2 cut(s) 100, 2067
BshFI GGCC 6 cut(s) 1043, 1079, 1159, 1602, 2389, 2613
BshNI GGYRCC 2 cut(s) 175, 1123
BshTI ACCGGT 2 cut(s) 1445, 1820
BshVI ATCGAT 1 cut(s) 677
BsiHKAI GWGCWC 3 cut(s) 29, 2305, 2475
BsiHKCI CYCGRG 2 cut(s) 1580, 1761
BsiSI CCGG 9 cut(s) 179, 460, 752, 846, 1133, 1269, 1446, 1821, 2614
BslFI GGGAC 3 cut(s) 993, 1969, 2008
BsmFI GGGAC 3 cut(s) 993, 1969, 2008
BsmI GAATGC 2 cut(s) 931, 1156
BsnI GGCC 6 cut(s) 1043, 1079, 1159, 1602, 2389, 2613
Bso31I GGTCTC 3 cut(s) 953, 1386, 1545
BsoBI CYCGRG 2 cut(s) 1580, 1761
Bsp120I GGGCCC 1 cut(s) 1077
Bsp1286I GDGCHC 5 cut(s) 29, 1081, 2129, 2305, 2475
Bsp13I TCCGGA 2 cut(s) 751, 845
Bsp19I CCATGG 2 cut(s) 1603, 2454
BspANI GGCC 6 cut(s) 1043, 1079, 1159, 1602, 2389, 2613
BspCNI CTCAG 9 cut(s) 468, 933, 1618, 1654, 1690, 1724, 2125, 2184, 2449
BspDI ATCGAT 1 cut(s) 677
BspEI TCCGGA 2 cut(s) 751, 845
BspFNI CGCG 2 cut(s) 100, 2067
BspHI TCATGA 1 cut(s) 2232
BspLI GGNNCC 7 cut(s) 177, 844, 1078, 1079, 1125, 1361, 1996
BspOI GCTAGC 1 cut(s) 2150
BspPI GGATC 4 cut(s) 837, 850, 1004, 2474
BspT107I GGYRCC 2 cut(s) 175, 1123
BspTNI GGTCTC 3 cut(s) 953, 1386, 1545
BsrDI GCAATG 5 cut(s) 1378, 1641, 2112, 2390, 2511
BsrFI RCCGGY 4 cut(s) 178, 1445, 1820, 2613
BsrI ACTGG 5 cut(s) 22, 1092, 1243, 1998, 2059
BssAI RCCGGY 4 cut(s) 178, 1445, 1820, 2613
BssECI CCNNGG 6 cut(s) 205, 641, 857, 1603, 2380, 2454
BssNAI GTATAC 1 cut(s) 108
BssNI GRCGYC 1 cut(s) 982
BssSI CACGAG 1 cut(s) 684
BssT1I CCWWGG 5 cut(s) 641, 857, 1603, 2380, 2454
Bst1107I GTATAC 1 cut(s) 108
Bst2BI CACGAG 1 cut(s) 684
Bst2UI CCWGG 5 cut(s) 576, 600, 657, 1476, 2120
Bst4CI ACNGT 7 cut(s) 136, 521, 1128, 1249, 1397, 1904, 2242
Bst6I CTCTTC 4 cut(s) 838, 858, 964, 1543
BstACI GRCGYC 1 cut(s) 982
BstAPI GCANNNNNTGC 2 cut(s) 1160, 2014
BstBAI YACGTR 1 cut(s) 2369
BstDSI CCRYGG 2 cut(s) 1603, 2454
BstENI CCTNNNNNAGG 1 cut(s) 324
BstF5I GGATG 7 cut(s) 229, 795, 1753, 2143, 2272, 2398, 2497
BstFNI CGCG 2 cut(s) 100, 2067
BstHHI GCGC 2 cut(s) 1658, 2257
BstMWI GCNNNNNNNGC 6 cut(s) 33, 299, 1160, 1630, 2014, 2316
BstNI CCWGG 5 cut(s) 576, 600, 657, 1476, 2120
BstNSI RCATGY 2 cut(s) 389, 1322
BstSCI CCNGG 6 cut(s) 459, 574, 598, 655, 1474, 2118
BstSFI CTRYAG 2 cut(s) 1785, 1863
BstSLI GKGCMC 1 cut(s) 1081
BstUI CGCG 2 cut(s) 100, 2067
BstV1I GCAGC 8 cut(s) 202, 298, 722, 902, 1642, 1933, 2020, 2506
BstV2I GAAGAC 2 cut(s) 388, 2488
BstX2I RGATCY 3 cut(s) 842, 1009, 1732
BstYI RGATCY 3 cut(s) 842, 1009, 1732
BstZ17I GTATAC 1 cut(s) 108
Bsu15I ATCGAT 1 cut(s) 677
Bsu36I CCTNAGG 1 cut(s) 920
BsuRI GGCC 6 cut(s) 1043, 1079, 1159, 1602, 2389, 2613
BsuTUI ATCGAT 1 cut(s) 677
BtgI CCRYGG 2 cut(s) 1603, 2454
BtrI CACGTC 1 cut(s) 2600
BtsCI GGATG 7 cut(s) 229, 795, 1753, 2143, 2272, 2398, 2497
BtsI GCAGTG 3 cut(s) 1614, 1679, 1996
BtsIMutI CAGTG 6 cut(s) 29, 1614, 1679, 1719, 1996, 2005
CaiI CAGNNNCTG 3 cut(s) 1160, 1421, 2119
CciI TCATGA 1 cut(s) 2232
CfoI GCGC 2 cut(s) 1658, 2257
Cfr10I RCCGGY 4 cut(s) 178, 1445, 1820, 2613
ClaI ATCGAT 1 cut(s) 677
CseI GACGC 3 cut(s) 33, 1107, 2073
CsiI ACCWGGT 1 cut(s) 1474
Csp6I GTAC 3 cut(s) 315, 522, 1782
CspAI ACCGGT 2 cut(s) 1445, 1820
CspCI CAANNNNNGTGG 2 cut(s) 1863, 1898
CviAII CATG 7 cut(s) 386, 1063, 1319, 1604, 1685, 2233, 2455
CviQI GTAC 3 cut(s) 315, 522, 1782
DriI GACNNNNNGTC 1 cut(s) 2001
Eam1104I CTCTTC 4 cut(s) 838, 858, 964, 1543
Eam1105I GACNNNNNGTC 1 cut(s) 2001
EarI CTCTTC 4 cut(s) 838, 858, 964, 1543
Ecl136II GAGCTC 1 cut(s) 2303
Eco130I CCWWGG 5 cut(s) 641, 857, 1603, 2380, 2454
Eco24I GRGCYC 3 cut(s) 1081, 2129, 2305
Eco31I GGTCTC 3 cut(s) 953, 1386, 1545
Eco32I GATATC 1 cut(s) 2605
Eco47I GGWCC 6 cut(s) 365, 1184, 1271, 1995, 2116, 2341
Eco53kI GAGCTC 1 cut(s) 2303
Eco57I CTGAAG 4 cut(s) 921, 990, 1944, 1965
Eco72I CACGTG 1 cut(s) 2369
Eco81I CCTNAGG 1 cut(s) 920
Eco88I CYCGRG 2 cut(s) 1580, 1761
EcoICRI GAGCTC 1 cut(s) 2303
EcoNI CCTNNNNNAGG 1 cut(s) 324
EcoO109I RGGNCCY 4 cut(s) 1077, 1078, 1157, 2116
EcoRII CCWGG 5 cut(s) 574, 598, 655, 1474, 2118
EcoRV GATATC 1 cut(s) 2605
EcoT14I CCWWGG 5 cut(s) 641, 857, 1603, 2380, 2454
EcoT38I GRGCYC 3 cut(s) 1081, 2129, 2305
ErhI CCWWGG 5 cut(s) 641, 857, 1603, 2380, 2454
FaeI CATG 7 cut(s) 389, 1066, 1322, 1607, 1688, 2236, 2458
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FaqI GGGAC 3 cut(s) 993, 1969, 2008
FatI CATG 7 cut(s) 385, 1062, 1318, 1603, 1684, 2232, 2454
FauI CCCGC 4 cut(s) 970, 1946, 2060, 2410
FbaI TGATCA 2 cut(s) 226, 2626
FblI GTMKAC 2 cut(s) 107, 1595
FokI GGATG 7 cut(s) 216, 802, 1740, 2150, 2279, 2385, 2504
FriOI GRGCYC 3 cut(s) 1081, 2129, 2305
FspBI CTAG 9 cut(s) 30, 162, 642, 873, 1671, 2147, 2334, 2360, 2540
FspI TGCGCA 2 cut(s) 1657, 2256
GlaI GCGC 2 cut(s) 1657, 2256
GsaI CCCAGC 1 cut(s) 1467
GsuI CTGGAG 7 cut(s) 42, 1007, 1109, 1830, 1836, 2141, 2189
HaeIII GGCC 6 cut(s) 1043, 1079, 1159, 1602, 2389, 2613
HapII CCGG 9 cut(s) 179, 460, 752, 846, 1133, 1269, 1446, 1821, 2614
HgaI GACGC 3 cut(s) 33, 1107, 2073
HhaI GCGC 2 cut(s) 1658, 2257
Hin1I GRCGYC 1 cut(s) 982
Hin1II CATG 7 cut(s) 389, 1066, 1322, 1607, 1688, 2236, 2458
Hin6I GCGC 2 cut(s) 1656, 2255
HinP1I GCGC 2 cut(s) 1656, 2255
HincII GTYRAC 1 cut(s) 2429
HindII GTYRAC 1 cut(s) 2429
HpaII CCGG 9 cut(s) 179, 460, 752, 846, 1133, 1269, 1446, 1821, 2614
HphI GGTGA 6 cut(s) 1208, 1329, 1460, 1496, 1835, 2172
Hpy99I CGWCG 2 cut(s) 49, 1132
HpyAV CCTTC 9 cut(s) 315, 330, 431, 741, 1373, 1921, 1967, 2442, 2603
HpyCH4III ACNGT 7 cut(s) 136, 521, 1128, 1249, 1397, 1904, 2242
HpyCH4IV ACGT 6 cut(s) 89, 104, 982, 1455, 2368, 2599
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 299, 1160, 1630, 2014, 2316
HpySE526I ACGT 6 cut(s) 89, 104, 982, 1455, 2368, 2599
Hsp92I GRCGYC 1 cut(s) 982
Hsp92II CATG 7 cut(s) 389, 1066, 1322, 1607, 1688, 2236, 2458
HspAI GCGC 2 cut(s) 1656, 2255
Kpn2I TCCGGA 2 cut(s) 751, 845
KroI GCCGGC 1 cut(s) 2613
KroNI GCCGGC 1 cut(s) 2615
Ksp22I TGATCA 2 cut(s) 226, 2626
LmnI GCTCC 9 cut(s) 307, 470, 793, 988, 1090, 1704, 1807, 2208, 2300
Lsp1109I GCAGC 8 cut(s) 202, 298, 722, 902, 1642, 1933, 2020, 2506
LweI GCATC 5 cut(s) 669, 1762, 1804, 1816, 2343
MabI ACCWGGT 1 cut(s) 1474
MaeI CTAG 9 cut(s) 30, 162, 642, 873, 1671, 2147, 2334, 2360, 2540
MaeII ACGT 6 cut(s) 89, 104, 982, 1455, 2368, 2599
MaeIII GTNAC 3 cut(s) 703, 1023, 1397
MflI RGATCY 3 cut(s) 842, 1009, 1732
MhlI GDGCHC 5 cut(s) 29, 1081, 2129, 2305, 2475
MluCI AATT 3 cut(s) 481, 1104, 1136
MlyI GAGTC 2 cut(s) 89, 1673
MmeI TCCRAC 4 cut(s) 27, 403, 2269, 2368
MroI TCCGGA 2 cut(s) 751, 845
MroNI GCCGGC 1 cut(s) 2613
MseI TTAA 6 cut(s) 18, 78, 417, 740, 783, 1305
MslI CAYNNNNRTG 3 cut(s) 770, 1317, 1619
MspA1I CMGCKG 1 cut(s) 193
MspI CCGG 9 cut(s) 179, 460, 752, 846, 1133, 1269, 1446, 1821, 2614
MspR9I CCNGG 6 cut(s) 461, 576, 600, 657, 1476, 2120
Mva1269I GAATGC 2 cut(s) 931, 1156
MvaI CCWGG 5 cut(s) 576, 600, 657, 1476, 2120
MvnI CGCG 2 cut(s) 100, 2067
MwoI GCNNNNNNNGC 6 cut(s) 33, 299, 1160, 1630, 2014, 2316
NaeI GCCGGC 1 cut(s) 2615
NciI CCSGG 1 cut(s) 461
NcoI CCATGG 2 cut(s) 1603, 2454
NgoMIV GCCGGC 1 cut(s) 2613
NheI GCTAGC 1 cut(s) 2146
NlaIII CATG 7 cut(s) 389, 1066, 1322, 1607, 1688, 2236, 2458
NlaIV GGNNCC 7 cut(s) 177, 844, 1078, 1079, 1125, 1361, 1996
NmuCI GTSAC 2 cut(s) 1023, 1397
NsbI TGCGCA 2 cut(s) 1657, 2256
NspI RCATGY 2 cut(s) 389, 1322
PaeR7I CTCGAG 1 cut(s) 1580
PagI TCATGA 1 cut(s) 2232
PciI ACATGT 1 cut(s) 385
PcsI WCGNNNNNNNCGW 1 cut(s) 557
PctI GAATGC 2 cut(s) 931, 1156
PdiI GCCGGC 1 cut(s) 2615
PfoI TCCNGGA 2 cut(s) 574, 2118
PinAI ACCGGT 2 cut(s) 1445, 1820
PleI GAGTC 2 cut(s) 89, 1672
PmaCI CACGTG 1 cut(s) 2369
PmlI CACGTG 1 cut(s) 2369
PpsI GAGTC 2 cut(s) 89, 1672
Ppu21I YACGTR 1 cut(s) 2369
PpuMI RGGWCCY 1 cut(s) 2116
PscI ACATGT 1 cut(s) 385
PshBI ATTAAT 2 cut(s) 783, 1305
Psp124BI GAGCTC 1 cut(s) 2305
Psp1406I AACGTT 2 cut(s) 89, 1455
Psp5II RGGWCCY 1 cut(s) 2116
Psp6I CCWGG 5 cut(s) 574, 598, 655, 1474, 2118
PspCI CACGTG 1 cut(s) 2369
PspFI CCCAGC 1 cut(s) 1463
PspGI CCWGG 5 cut(s) 574, 598, 655, 1474, 2118
PspN4I GGNNCC 7 cut(s) 177, 844, 1078, 1079, 1125, 1361, 1996
PspOMI GGGCCC 1 cut(s) 1077
PspPPI RGGWCCY 1 cut(s) 2116
PsrI GAACNNNNNNTAC 2 cut(s) 2026, 2058
PstNI CAGNNNCTG 3 cut(s) 1160, 1421, 2119
PsuI RGATCY 3 cut(s) 842, 1009, 1732
RsaI GTAC 3 cut(s) 316, 523, 1783
RsaNI GTAC 3 cut(s) 315, 522, 1782
RseI CAYNNNNRTG 3 cut(s) 770, 1317, 1619
SacI GAGCTC 1 cut(s) 2305
SaqAI TTAA 6 cut(s) 18, 78, 417, 740, 783, 1305
ScaI AGTACT 1 cut(s) 523
SchI GAGTC 2 cut(s) 89, 1673
ScrFI CCNGG 6 cut(s) 461, 576, 600, 657, 1476, 2120
SduI GDGCHC 5 cut(s) 29, 1081, 2129, 2305, 2475
SexAI ACCWGGT 1 cut(s) 1474
SfaNI GCATC 5 cut(s) 669, 1762, 1804, 1816, 2343
SfcI CTRYAG 2 cut(s) 1785, 1863
Sfr274I CTCGAG 1 cut(s) 1580
SinI GGWCC 6 cut(s) 365, 1184, 1271, 1995, 2116, 2341
SlaI CTCGAG 1 cut(s) 1580
SmiMI CAYNNNNRTG 3 cut(s) 770, 1317, 1619
SmlI CTYRAG 5 cut(s) 71, 1580, 2098, 2304, 2574
SmoI CTYRAG 5 cut(s) 71, 1580, 2098, 2304, 2574
SpeI ACTAGT 1 cut(s) 2539
Sse9I AATT 3 cut(s) 481, 1104, 1136
SspI AATATT 1 cut(s) 1309
SspMI CTAG 9 cut(s) 30, 162, 642, 873, 1671, 2147, 2334, 2360, 2540
SstI GAGCTC 1 cut(s) 2305
StyD4I CCNGG 6 cut(s) 459, 574, 598, 655, 1474, 2118
StyI CCWWGG 5 cut(s) 641, 857, 1603, 2380, 2454
TaaI ACNGT 7 cut(s) 136, 521, 1128, 1249, 1397, 1904, 2242
TaiI ACGT 6 cut(s) 92, 107, 985, 1458, 2371, 2602
TaqI TCGA 7 cut(s) 373, 551, 677, 1581, 2161, 2511, 2607
TaqII GACCGA 1 cut(s) 1201
TasI AATT 3 cut(s) 481, 1104, 1136
TatI WGTACW 2 cut(s) 521, 1781
TauI GCSGC 4 cut(s) 196, 1427, 2319, 2322
Tru1I TTAA 6 cut(s) 18, 78, 417, 740, 783, 1305
Tru9I TTAA 6 cut(s) 18, 78, 417, 740, 783, 1305
TscAI CASTG 6 cut(s) 29, 1621, 1686, 1719, 1996, 2005
TseFI GTSAC 2 cut(s) 1023, 1397
TseI GCWGC 8 cut(s) 190, 286, 710, 890, 1630, 1921, 2008, 2519
Tsp45I GTSAC 2 cut(s) 1023, 1397
TspDTI ATGAA 7 cut(s) 144, 723, 769, 990, 1740, 2249, 2577
TspGWI ACGGA 2 cut(s) 1794, 2489
TspRI CASTG 6 cut(s) 29, 1621, 1686, 1719, 1996, 2005
VpaK11BI GGWCC 6 cut(s) 365, 1184, 1271, 1995, 2116, 2341
VspI ATTAAT 2 cut(s) 783, 1305
XagI CCTNNNNNAGG 1 cut(s) 324
XapI RAATTY 1 cut(s) 481
XbaI TCTAGA 1 cut(s) 2333
XceI RCATGY 2 cut(s) 389, 1322
XcmI CCANNNNNNNNNTGG 1 cut(s) 1370
XhoI CTCGAG 1 cut(s) 1580
XmaJI CCTAGG 1 cut(s) 641
XmiI GTMKAC 2 cut(s) 107, 1595
XspI CTAG 9 cut(s) 30, 162, 642, 873, 1671, 2147, 2334, 2360, 2540
ZraI GACGTC 1 cut(s) 983
ZrmI AGTACT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.