MD08G1004700.v1.1

Large proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005387
Physical Location & Seq
Forward (+)
432245 .. 439440
7196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1004700.v1.1.491

Sequence Viewer

Length: 2709 bp
ATGGCAGATCAGCACTCCAATGAAGGCACCAGTTCCGGCAATGTTGCTGGGGCTGATTGTGATTCAAATCTTGAGATCAATATCAAGACTTTAGACTCACAGATGTATAGTTTTCAAGTTGATAAAAATATGCGAGTTTCTTTGTTCAAGGAAAAAATAGCTGATCAAACAGGTGTTCCAGTTGGTCAGCAGCGGCTGATTTTCAGGGGAAGAGTGTTAAAAGATGACCATCCTCTTTCTGAGTATCATTTGGAAAATGGGCACACATTGCACTTAGTTATAAGGCAGCCATCCCAGCCACAACCTTCGTCGGGTACAAGTTCTGGCGAGGCACATGCTAATAGTGGAAATGATCCTAGTGGTGTTCCTCGTGGTCGCATTGGCCAGATATCACACAGTGTAGTTCTTGGGACGTTTAATGTTGGAGATCAAGGTGAAGGCGCTGTTCCTGATCTTAATCGGGTTATTGGCGCAGTTTTGAATTCTATTGGAATTGGCACCCAGGCTACAACTAATGGCACAGGCAACCCACAATTCACTACTTCGGTGAGCAATTTGAACACTCCTGGTCAACCTCCTAATGGAAATGAAGCAGAAGGGTCGCGTAATGTCAACCGAGGAGGACATCAAGCACAGTATGGACAGGCATTTCCTACTCAACCATTCCAAACTTTTCCTCAGGCTGTGCAACCTCCTCATGCAGCTGCAGCATTTCCCATGCCTTCATCAAATATGCCAATTCCACACTCTTTGAATACTCTCTCCGAGTTTATGAACAGAATGGAGCAGGGATTATCGCAAAATGGTTATCAGCCAAATACATCTGCAACCAATACAGGAGAACCACCTAGGGTGAACTTACCTTCCACTGCAGAGGGGATGCCTACACCTGAGGCATTGGGGATTGTTCTTCGTCATGCAGAGCGACTTCTCAGCAATCATGCTGTTTCTGCATTATCTCACATTGCAGGACGCTTGGAACAAGAGGGAGTGTCTCCTGATCCCTCTGTGAGAGGCCAAATTCAGACAGAATCTATACAATTAGGGCTTGCAATGCAACACTTAGGTTCTCTTCTTCTTGAGCTTGGCCGAACAATTTGGACGCTTCGTATGGGGCAGTCTCCTGGAGAAGCTGTTGTGAATTCTGGGCCAGCAGTTTATATATCCCCAGCAGGGCCTAATCCCATAATGGTCCAGCCTTTCCCTCTTCAAACAAGTTCACTCTTTGGTGGTTCTGTACCTCAACCAAACCCTACGAGTTTTGGTCCTGTTGGGATTGGAAGTGCTCCAAGGAATGTAAACATCCATATACATGCTGTTGGTGCTAGGGGTAGTAATGGGGAGGGTATCCCATCTGGTTCTAGGGATTCAGGTGGCCGGGTGCTGCCAGTGAGAAATGTAGTTGGTGCCACTATTCCATCCTCCCAAACTGGTGTTGCAGTCTCCGCTGCGTCGCAACCCGGTCCTGGTGGTTCTGTTTCACAGCCGCCTTCTGGCTCCCCGCTTTCCTCCATTGTTGCTGAACTTAACTCACATATTGGCAATGCACAACCAGATGACACAGCTCAATCAGGAAGTGAACGGCCAAGTACTGACTATGTCGATGTAGCTGGGCAGTCTAGTGTATCACTACCTGGTTGTACATCTGAAAGTGAAGGTCTGAAGGATTTAGCAAGTGGTAGTACATTGAAAAACGATTCAAGATCCCCAGCTGGGGGTCCTCTAAGCTCTTCAAGTGGCCAAAACACCGTGGTGAGAGAAGATGAGAAGAGAAGTGCTCCACAATCCAGTGAGAAGCAGGCTGAGGGTGCGAAAGCTGTTCCACTTGGTTTAGGACTTGGAGTTTTGGAGCGTAAGAGACAAGCCAGGCAGCCAAAACCGTCAACCAAGAATGGAGATGGCGGAACAACTAGTGCTCCCATCAATCAGAATCAGCAAGTAATAGGTGGCCAGCAAGTTTTGCAATCCCTTGCGTCTCGTGGTTCTGCTGCAAGTAGGATGAACTTAAATGATGCACCTGAAAGACAAACGGCCCCTGCTGTTGGTCAGGTCAGAGATGGTAGAACATTGGGAGGGCAAGGTCCTCTTGGCCAAGTTGATATGGGAAGCATGATGTCTCAGGTTCTGCAGAGTCCAGCCCTGAATGGTCTGTTGGCAGGTGTTTCAGACCAAACTGGAGTGGGTTCTCCCGATGTATTGAGGAATATGTTACAGAGCTTTACACAGAATCCACAGATGAGGAGTGCTGTCAATCAGATTGCTGAACAGGTTGACGGCCAAGATTTGGGAAACTTGTTTGGAGGAGGCCAAGGTGGGGGGATCGATCTGTCCAGGATGTTCCAACAAATGATGCCCATTGTAACTCGTGCCCTTGGGGCTGGATCGCCCCCCGTCCGGCCATCCTCTGCTTTGGCTCCTGAATCTCATCCGCCCCACAATGAGCGAAGTCTCAGTAGAGATGATAATATCCCTAATTCTGAGATTAACCTCGAAGAAGTGGTTCAGAGAATTGAGAACCTGAATGCACCAGGAGAAGTTTTTCATGCTTTAGTTGAAAATTCGGTGCAGCTGTCTGGCAGAGGAAGCGGTTCTCAAGAACTTGTGGATGAGTTGTGCAGGGACGAGAGTCTCTCCAGCGAATATGTTGAAATGTTAAGTAGGGACATACGGCGACGACTTGAGGGTGATTCGGGGAAGGACAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001822 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010605 GO:0010941 GO:0012501 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031593 GO:0031647 GO:0032268 GO:0032269 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042981 GO:0043021 GO:0043022 GO:0043067 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045861 GO:0045995 GO:0048232 GO:0048285 GO:0048513 GO:0048519 GO:0048523 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070059 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1903046 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

903

Amino Acids

94.09

Weight (kDa)

5.61

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 25 - 94 3e-09 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 26 - 96 2e-23 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 281
AarI CACCTGC 1 cut(s) 2147
Acc36I ACCTGC 1 cut(s) 2147
AccB1I GGYRCC 3 cut(s) 26, 497, 1406
AccB7I CCANNNNNTGG 1 cut(s) 2284
AccII CGCG 1 cut(s) 604
AciI CCGC 7 cut(s) 193, 1446, 1487, 1502, 1902, 2429, 2586
AclWI GGATC 5 cut(s) 347, 995, 1698, 2327, 2389
AcoI YGGCCR 9 cut(s) 382, 1087, 1375, 1583, 1738, 1948, 2089, 2275, 2396
AcsI RAATTY 4 cut(s) 481, 1020, 1141, 2557
AcuI CTGAAG 1 cut(s) 1682
AdeI CACNNNGTG 1 cut(s) 398
AfaI GTAC 5 cut(s) 316, 1239, 1591, 1642, 1684
AhlI ACTAGT 1 cut(s) 1910
AjnI CCWGG 8 cut(s) 501, 565, 1123, 1465, 1633, 1865, 2330, 2527
AjuI GAANNNNNNNTTGG 2 cut(s) 2135, 2167
AleI CACNNNNGTG 1 cut(s) 1751
AloI GAACNNNNNNTCC 2 cut(s) 848, 880
Alw21I GWGCWC 3 cut(s) 1288, 1780, 1918
Alw26I GTCTC 8 cut(s) 999, 1125, 1447, 1852, 1980, 2121, 2453, 2633
AlwI GGATC 5 cut(s) 347, 995, 1698, 2327, 2389
AlwNI CAGNNNCTG 2 cut(s) 196, 2125
ApoI RAATTY 4 cut(s) 481, 1020, 1141, 2557
Asp700I GAANNNNTTC 3 cut(s) 2499, 2538, 2587
AspA2I CCTAGG 1 cut(s) 848
AspLEI GCGC 2 cut(s) 443, 473
AspS9I GGNCC 8 cut(s) 1148, 1175, 1192, 1265, 1463, 1718, 2032, 2081
AsuC2I CCSGG 2 cut(s) 1379, 1461
AsuHPI GGTGA 5 cut(s) 446, 559, 865, 1765, 2696
AvaII GGWCC 5 cut(s) 1192, 1265, 1463, 1718, 2081
AvrII CCTAGG 1 cut(s) 848
AxyI CCTNAGG 2 cut(s) 678, 891
BaeGI GKGCMC 2 cut(s) 264, 2371
BalI TGGCCA 4 cut(s) 384, 1740, 1950, 2091
BanI GGYRCC 3 cut(s) 26, 497, 1406
BauI CACGAG 3 cut(s) 369, 1977, 2364
Bbv12I GWGCWC 3 cut(s) 1288, 1780, 1918
BbvCI CCTCAGC 1 cut(s) 1803
BccI CCATC 8 cut(s) 237, 298, 1360, 1426, 1892, 1928, 2051, 2407
BceAI ACGGC 4 cut(s) 1598, 2046, 2290, 2684
BcgI CGANNNNNNTGC 4 cut(s) 317, 351, 582, 616
BciT130I CCWGG 8 cut(s) 503, 567, 1125, 1467, 1635, 1867, 2332, 2529
BciVI GTATCC 1 cut(s) 1358
BclI TGATCA 1 cut(s) 163
BcnI CCSGG 2 cut(s) 1379, 1461
BcoDI GTCTC 8 cut(s) 999, 1125, 1447, 1852, 1980, 2121, 2453, 2633
BcuI ACTAGT 1 cut(s) 1910
BfaI CTAG 6 cut(s) 357, 849, 1326, 1362, 1620, 1911
BfmI CTRYAG 3 cut(s) 705, 870, 2126
BfoI RGCGCY 1 cut(s) 444
BfuAI ACCTGC 1 cut(s) 2147
BfuI GTATCC 1 cut(s) 1358
BglI GCCNNNNNGGC 1 cut(s) 2375
BlnI CCTAGG 1 cut(s) 848
BmcAI AGTACT 1 cut(s) 1591
Bme18I GGWCC 5 cut(s) 1192, 1265, 1463, 1718, 2081
BmgT120I GGNCC 8 cut(s) 1148, 1175, 1192, 1265, 1463, 1718, 2032, 2081
BmiI GGNNCC 7 cut(s) 28, 499, 1408, 1498, 1719, 2034, 2415
BmsI GCATC 3 cut(s) 870, 2002, 2340
BoxI GACNNNNGTC 1 cut(s) 2625
BplI GAGNNNNNCTC 4 cut(s) 1762, 1794, 2615, 2647
BpmI CTGGAG 3 cut(s) 1146, 2196, 2617
Bpu10I CCTNAGC 1 cut(s) 1803
BpuEI CTTGAG 4 cut(s) 92, 1100, 2577, 2699
BpuMI CCSGG 2 cut(s) 1379, 1461
Bsa29I ATCGAT 1 cut(s) 2322
BsaBI GATNNNNATC 4 cut(s) 66, 80, 228, 456
BsaJI CCNNGG 7 cut(s) 501, 616, 848, 1289, 1749, 2308, 2371
BsaXI ACNNNNNCTCC 4 cut(s) 1119, 1149, 1900, 1930
Bse1I ACTGG 6 cut(s) 30, 179, 1388, 1435, 1788, 2179
Bse21I CCTNAGG 2 cut(s) 678, 891
Bse3DI GCAATG 5 cut(s) 46, 266, 963, 1059, 1549
Bse8I GATNNNNATC 4 cut(s) 66, 80, 228, 456
BseBI CCWGG 8 cut(s) 503, 567, 1125, 1467, 1635, 1867, 2332, 2529
BseCI ATCGAT 1 cut(s) 2322
BseDI CCNNGG 7 cut(s) 501, 616, 848, 1289, 1749, 2308, 2371
BseJI GATNNNNATC 4 cut(s) 66, 80, 228, 456
BseMI GCAATG 5 cut(s) 46, 266, 963, 1059, 1549
BseMII CTCAG 8 cut(s) 231, 692, 882, 946, 1794, 2132, 2464, 2469
BseNI ACTGG 6 cut(s) 30, 179, 1388, 1435, 1788, 2179
BseRI GAGGAG 4 cut(s) 633, 684, 2254, 2316
BseSI GKGCMC 2 cut(s) 264, 2371
BseYI CCCAGC 6 cut(s) 47, 294, 1168, 1610, 1708, 1712
BsgI GTGCAG 2 cut(s) 2585, 2635
Bsh1236I CGCG 1 cut(s) 604
BshNI GGYRCC 3 cut(s) 26, 497, 1406
BshVI ATCGAT 1 cut(s) 2322
BsiHKAI GWGCWC 3 cut(s) 1288, 1780, 1918
BsiSI CCGG 4 cut(s) 36, 1378, 1461, 2395
BslFI GGGAC 3 cut(s) 424, 2633, 2675
BsmAI GTCTC 8 cut(s) 999, 1125, 1447, 1852, 1980, 2121, 2453, 2633
BsmBI CGTCTC 1 cut(s) 1980
BsmFI GGGAC 3 cut(s) 424, 2633, 2675
BsmI GAATGC 1 cut(s) 2527
Bsp1286I GDGCHC 5 cut(s) 264, 1288, 1780, 1918, 2371
Bsp1407I TGTACA 1 cut(s) 1640
BspACI CCGC 7 cut(s) 193, 1446, 1487, 1502, 1902, 2429, 2586
BspCNI CTCAG 8 cut(s) 232, 691, 883, 945, 1795, 2131, 2463, 2470
BspDI ATCGAT 1 cut(s) 2322
BspFNI CGCG 1 cut(s) 604
BspLI GGNNCC 7 cut(s) 28, 499, 1408, 1498, 1719, 2034, 2415
BspMAI CTGCAG 3 cut(s) 709, 874, 2130
BspMI ACCTGC 1 cut(s) 2147
BspPI GGATC 5 cut(s) 347, 995, 1698, 2327, 2389
BspQI GCTCTTC 1 cut(s) 1735
BspT107I GGYRCC 3 cut(s) 26, 497, 1406
BsrDI GCAATG 5 cut(s) 46, 266, 963, 1059, 1549
BsrGI TGTACA 1 cut(s) 1640
BsrI ACTGG 6 cut(s) 30, 179, 1388, 1435, 1788, 2179
BssECI CCNNGG 7 cut(s) 501, 616, 848, 1289, 1749, 2308, 2371
BssSI CACGAG 3 cut(s) 369, 1977, 2364
BssT1I CCWWGG 4 cut(s) 848, 1289, 2308, 2371
Bst2BI CACGAG 3 cut(s) 369, 1977, 2364
Bst2UI CCWGG 8 cut(s) 503, 567, 1125, 1467, 1635, 1867, 2332, 2529
Bst4CI ACNGT 4 cut(s) 398, 636, 1750, 1881
Bst6I CTCTTC 5 cut(s) 205, 1077, 1212, 1735, 1763
BstAPI GCANNNNNTGC 2 cut(s) 268, 1960
BstAUI TGTACA 1 cut(s) 1640
BstC8I GCNNGC 4 cut(s) 1050, 1152, 1800, 1952
BstDSI CCRYGG 1 cut(s) 1749
BstFNI CGCG 1 cut(s) 604
BstH2I RGCGCY 1 cut(s) 444
BstHHI GCGC 2 cut(s) 443, 473
BstMAI GTCTC 8 cut(s) 999, 1125, 1447, 1852, 1980, 2121, 2453, 2633
BstNI CCWGG 8 cut(s) 503, 567, 1125, 1467, 1635, 1867, 2332, 2529
BstNSI RCATGY 2 cut(s) 338, 1316
BstPAI GACNNNNGTC 1 cut(s) 2625
BstSFI CTRYAG 3 cut(s) 705, 870, 2126
BstSLI GKGCMC 2 cut(s) 264, 2371
BstUI CGCG 1 cut(s) 604
BstX2I RGATCY 1 cut(s) 1703
BstYI RGATCY 1 cut(s) 1703
Bsu15I ATCGAT 1 cut(s) 2322
Bsu36I CCTNAGG 2 cut(s) 678, 891
BsuI GTATCC 1 cut(s) 1358
BsuTUI ATCGAT 1 cut(s) 2322
BtgI CCRYGG 1 cut(s) 1749
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 4 cut(s) 403, 867, 1395, 1795
BveI ACCTGC 1 cut(s) 2147
Cac8I GCNNGC 4 cut(s) 1050, 1152, 1800, 1952
CaiI CAGNNNCTG 2 cut(s) 196, 2125
CfoI GCGC 2 cut(s) 443, 473
Cfr13I GGNCC 8 cut(s) 1148, 1175, 1192, 1265, 1463, 1718, 2032, 2081
ClaI ATCGAT 1 cut(s) 2322
CseI GACGC 4 cut(s) 981, 1111, 1440, 1962
CsiI ACCWGGT 1 cut(s) 1633
Csp6I GTAC 5 cut(s) 315, 1238, 1590, 1641, 1683
CspCI CAANNNNNGTGG 2 cut(s) 732, 767
CviAII CATG 8 cut(s) 335, 698, 718, 917, 941, 1313, 2110, 2543
CviQI GTAC 5 cut(s) 315, 1238, 1590, 1641, 1683
DraIII CACNNNGTG 1 cut(s) 398
EaeI YGGCCR 9 cut(s) 382, 1087, 1375, 1583, 1738, 1948, 2089, 2275, 2396
Eam1104I CTCTTC 5 cut(s) 205, 1077, 1212, 1735, 1763
EarI CTCTTC 5 cut(s) 205, 1077, 1212, 1735, 1763
EciI GGCGGA 2 cut(s) 1917, 2418
Eco130I CCWWGG 4 cut(s) 848, 1289, 2308, 2371
Eco32I GATATC 1 cut(s) 390
Eco47I GGWCC 5 cut(s) 1192, 1265, 1463, 1718, 2081
Eco57I CTGAAG 1 cut(s) 1682
Eco81I CCTNAGG 2 cut(s) 678, 891
EcoO109I RGGNCCY 3 cut(s) 1175, 1718, 2081
EcoRI GAATTC 2 cut(s) 481, 1141
EcoRII CCWGG 8 cut(s) 501, 565, 1123, 1465, 1633, 1865, 2330, 2527
EcoRV GATATC 1 cut(s) 390
EcoT14I CCWWGG 4 cut(s) 848, 1289, 2308, 2371
ErhI CCWWGG 4 cut(s) 848, 1289, 2308, 2371
Esp3I CGTCTC 1 cut(s) 1980
FaeI CATG 8 cut(s) 338, 701, 721, 920, 944, 1316, 2113, 2546
FalI AAGNNNNNCTT 2 cut(s) 2070, 2102
FaqI GGGAC 3 cut(s) 424, 2633, 2675
FatI CATG 8 cut(s) 334, 697, 717, 916, 940, 1312, 2109, 2542
FauI CCCGC 1 cut(s) 1509
FbaI TGATCA 1 cut(s) 163
FspBI CTAG 6 cut(s) 357, 849, 1326, 1362, 1620, 1911
GlaI GCGC 2 cut(s) 442, 472
GsaI CCCAGC 6 cut(s) 51, 298, 1172, 1614, 1712, 1716
GsuI CTGGAG 3 cut(s) 1146, 2196, 2617
HaeII RGCGCY 1 cut(s) 444
HapII CCGG 4 cut(s) 36, 1378, 1461, 2395
HgaI GACGC 4 cut(s) 981, 1111, 1440, 1962
HhaI GCGC 2 cut(s) 443, 473
Hin1II CATG 8 cut(s) 338, 701, 721, 920, 944, 1316, 2113, 2546
Hin6I GCGC 2 cut(s) 441, 471
HinP1I GCGC 2 cut(s) 441, 471
HincII GTYRAC 4 cut(s) 572, 613, 1884, 2272
HindII GTYRAC 4 cut(s) 572, 613, 1884, 2272
HpaII CCGG 4 cut(s) 36, 1378, 1461, 2395
HphI GGTGA 5 cut(s) 446, 559, 865, 1765, 2696
Hpy166II GTNNAC 8 cut(s) 572, 613, 856, 1220, 1300, 1580, 1884, 2272
Hpy8I GTNNAC 8 cut(s) 572, 613, 856, 1220, 1300, 1580, 1884, 2272
Hpy99I CGWCG 3 cut(s) 313, 1456, 2676
HpyCH4III ACNGT 4 cut(s) 398, 636, 1750, 1881
HpyCH4IV ACGT 1 cut(s) 413
HpySE526I ACGT 1 cut(s) 413
Hsp92II CATG 8 cut(s) 338, 701, 721, 920, 944, 1316, 2113, 2546
HspAI GCGC 2 cut(s) 441, 471
Ksp22I TGATCA 1 cut(s) 163
LguI GCTCTTC 1 cut(s) 1735
LmnI GCTCC 7 cut(s) 784, 1291, 1502, 1783, 1849, 1921, 2419
LweI GCATC 3 cut(s) 870, 2002, 2340
MabI ACCWGGT 1 cut(s) 1633
MaeI CTAG 6 cut(s) 357, 849, 1326, 1362, 1620, 1911
MaeII ACGT 1 cut(s) 413
MaeIII GTNAC 2 cut(s) 2208, 2359
MboII GAAGA 9 cut(s) 222, 902, 1064, 1067, 1199, 1722, 1772, 1780, 2504
MflI RGATCY 1 cut(s) 1703
MhlI GDGCHC 5 cut(s) 264, 1288, 1780, 1918, 2371
MlsI TGGCCA 4 cut(s) 384, 1740, 1950, 2091
MluNI TGGCCA 4 cut(s) 384, 1740, 1950, 2091
MlyI GAGTC 3 cut(s) 89, 2140, 2635
MmeI TCCRAC 2 cut(s) 403, 2365
Mox20I TGGCCA 4 cut(s) 384, 1740, 1950, 2091
MroXI GAANNNNTTC 3 cut(s) 2499, 2538, 2587
MscI TGGCCA 4 cut(s) 384, 1740, 1950, 2091
MseI TTAA 7 cut(s) 218, 417, 456, 1527, 2006, 2484, 2654
MslI CAYNNNNRTG 3 cut(s) 18, 1311, 1751
Msp20I TGGCCA 4 cut(s) 384, 1740, 1950, 2091
MspA1I CMGCKG 5 cut(s) 193, 704, 1448, 1712, 2569
MspI CCGG 4 cut(s) 36, 1378, 1461, 2395
Mva1269I GAATGC 1 cut(s) 2527
MvaI CCWGG 8 cut(s) 503, 567, 1125, 1467, 1635, 1867, 2332, 2529
MvnI CGCG 1 cut(s) 604
NciI CCSGG 2 cut(s) 1379, 1461
NlaIII CATG 8 cut(s) 338, 701, 721, 920, 944, 1316, 2113, 2546
NlaIV GGNNCC 7 cut(s) 28, 499, 1408, 1498, 1719, 2034, 2415
NspI RCATGY 2 cut(s) 338, 1316
OliI CACNNNNGTG 1 cut(s) 1751
PaqCI CACCTGC 1 cut(s) 2147
PciSI GCTCTTC 1 cut(s) 1735
PctI GAATGC 1 cut(s) 2527
PdmI GAANNNNTTC 3 cut(s) 2499, 2538, 2587
PfeI GAWTC 8 cut(s) 62, 1031, 1367, 1697, 1930, 2227, 2420, 2687
PflFI GACNNNGTC 1 cut(s) 1598
PflMI CCANNNNNTGG 1 cut(s) 2284
PfoI TCCNGGA 2 cut(s) 1123, 2330
PleI GAGTC 3 cut(s) 89, 2139, 2634
PpsI GAGTC 3 cut(s) 89, 2139, 2634
PpuMI RGGWCCY 2 cut(s) 1718, 2081
PshAI GACNNNNGTC 1 cut(s) 2625
PsiI TTATAA 1 cut(s) 281
Psp5II RGGWCCY 2 cut(s) 1718, 2081
Psp6I CCWGG 8 cut(s) 501, 565, 1123, 1465, 1633, 1865, 2330, 2527
PspFI CCCAGC 6 cut(s) 47, 294, 1168, 1610, 1708, 1712
PspGI CCWGG 8 cut(s) 501, 565, 1123, 1465, 1633, 1865, 2330, 2527
PspN4I GGNNCC 7 cut(s) 28, 499, 1408, 1498, 1719, 2034, 2415
PspPI GGNCC 8 cut(s) 1148, 1175, 1192, 1265, 1463, 1718, 2032, 2081
PspPPI RGGWCCY 2 cut(s) 1718, 2081
PstI CTGCAG 3 cut(s) 709, 874, 2130
PstNI CAGNNNCTG 2 cut(s) 196, 2125
PsuI RGATCY 1 cut(s) 1703
PsyI GACNNNGTC 1 cut(s) 1598
PvuII CAGCTG 3 cut(s) 704, 1712, 2569
RsaI GTAC 5 cut(s) 316, 1239, 1591, 1642, 1684
RsaNI GTAC 5 cut(s) 315, 1238, 1590, 1641, 1683
RseI CAYNNNNRTG 3 cut(s) 18, 1311, 1751
SapI GCTCTTC 1 cut(s) 1735
SaqAI TTAA 7 cut(s) 218, 417, 456, 1527, 2006, 2484, 2654
Sau96I GGNCC 8 cut(s) 1148, 1175, 1192, 1265, 1463, 1718, 2032, 2081
ScaI AGTACT 1 cut(s) 1591
SchI GAGTC 3 cut(s) 89, 2140, 2635
SduI GDGCHC 5 cut(s) 264, 1288, 1780, 1918, 2371
SexAI ACCWGGT 1 cut(s) 1633
SfaNI GCATC 3 cut(s) 870, 2002, 2340
SfcI CTRYAG 3 cut(s) 705, 870, 2126
SinI GGWCC 5 cut(s) 1192, 1265, 1463, 1718, 2081
SmiMI CAYNNNNRTG 3 cut(s) 18, 1311, 1751
SmlI CTYRAG 4 cut(s) 71, 1079, 2592, 2678
SmoI CTYRAG 4 cut(s) 71, 1079, 2592, 2678
SpeI ACTAGT 1 cut(s) 1910
SsiI CCGC 7 cut(s) 193, 1446, 1487, 1502, 1902, 2429, 2586
SspMI CTAG 6 cut(s) 357, 849, 1326, 1362, 1620, 1911
StyI CCWWGG 4 cut(s) 848, 1289, 2308, 2371
TaaI ACNGT 4 cut(s) 398, 636, 1750, 1881
TaiI ACGT 1 cut(s) 416
TaqI TCGA 3 cut(s) 1602, 2322, 2490
TatI WGTACW 3 cut(s) 1589, 1640, 1682
TauI GCSGC 2 cut(s) 196, 1489
TfiI GAWTC 8 cut(s) 62, 1031, 1367, 1697, 1930, 2227, 2420, 2687
Tru1I TTAA 7 cut(s) 218, 417, 456, 1527, 2006, 2484, 2654
Tru9I TTAA 7 cut(s) 218, 417, 456, 1527, 2006, 2484, 2654
TscAI CASTG 4 cut(s) 403, 874, 1395, 1795
TspDTI ATGAA 6 cut(s) 36, 603, 714, 788, 2015, 2531
TspRI CASTG 4 cut(s) 403, 874, 1395, 1795
Tth111I GACNNNGTC 1 cut(s) 1598
Van91I CCANNNNNTGG 1 cut(s) 2284
VpaK11BI GGWCC 5 cut(s) 1192, 1265, 1463, 1718, 2081
XapI RAATTY 4 cut(s) 481, 1020, 1141, 2557
XceI RCATGY 2 cut(s) 338, 1316
XmaJI CCTAGG 1 cut(s) 848
XmnI GAANNNNTTC 3 cut(s) 2499, 2538, 2587
XspI CTAG 6 cut(s) 357, 849, 1326, 1362, 1620, 1911
ZrmI AGTACT 1 cut(s) 1591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.