pycom08g00350

Large proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005387
Physical Location & Seq
Forward (+)
337503 .. 343486
5984 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00350.6

Sequence Viewer

Length: 2667 bp
ATGTATAGTTTTCAAGTTGATAAAAATATGCGAGTTTCATTGTTCAAGGAAAAAATAGCTGATCAAACAGGTGTTCCAGTTGGTCAGCAGCGGCTGATTTTCAGGGGAAGAGTGTTAAAAGATGACCATCCTCTTTCTGAGTATCATTTGGAAAATGGGCACACATTGCACTTAGTTATAAGGCAGCCGTCCCAGCCACAACCTTCGTCGGGTACAAGTTCTGGCGAGGCACATGCAAATAGTGGAAATGATCCTAGTGGTGTTCCTCGTGGTCGCATTGGCCAGATATCACACAGTGTAGTTCTTGGGACGTTTAATGTTGGAGATCAAGGTGAAGGTGCTGTTCCTGATCTTAATCGGGTTATTGGCGCAGTTCTCAATTCTATTGGAATTGGCACCCAGGCTACAACTAATGGCACAGGCAACCCACAATTCACTACTTCGGTGAGCAATTTAAACACTCCTGGTCAGCCTCCTAATGGAAATGAAGCAGAAGGGTTGCATAATGTCAACCGAGGAGGACATCAAGCACAGTCTGGACAGGCATTTCCTACTCAACCATTCCAAACTTTTCCTCAGGCTGTGCAACCTCCTCATGCAGCTGCAGCATTTCCCATGCCTCCATCAAATATGCCAATTCCACACTCTTTGAATACTCTTTCGGAGTTTATGAACAGAATGGAGCAGGGATTATCGCAAAATGGTTATCAGCCAAATACATCTGCAACCAATACAGGAGAACCGCCTAGGGTGAACTTACCTTCCACTGCAGGGGGGATGCCAACACCTGAGGCATTGGGGATTGTTCTTCGTCATGCAGAGCGACTTCTCAGCGATCATGCTGTTTCTGCACTATCTCACATTGCAGGACGCTTGGAACAAGAGGGAGTGTCTCCTGATTCCTCTGTGAGAAGCCAAATTCAGACAGAATCTATACAATTAGGGCTTGCAATGCAACACTTAGGTTCTCTTCTTCTTGAGCTTGGCCGAACGATTTGGACGCTTCGTATGGGGCAGTCTCCTGGAGAAGCTGTTGTGAATGCTGGGCCAGCAGTTTATATATCCCCAGCAGGGCCTAATCCCATAATGGTCCAGCCTTTCCCTCTTCAAACAAGCTCACTCTTTGGTGGTTCTGTACCTCAATCAAACCCTACGAGTTTTGGTCCTGTTGGGATTGGAAGTGCTCCAAGGAATGTAAACATCCATATACATGCTGTTGGTGCTAGGGGTAGTAATGGGGAGGGTATCCCCTCTGGTTCTAGGGATTCAGGTGGCCAGGTGCTGCCAGTGAGAAATGTAGTTGGTGCCAGTATTCCATCCTCCCAAACTGGTGTCGCAGTCTCCTCTGCATCGCAACCCGGTCCTGGTGGTTCTGTTTCACAGCCGCCTTCTGGCTCCTCGCTTTCCTCCATTGTTGATGAACTTAATTCACATATTAGAAATTTTGTTGGCAATGCACAACCAGATGACACAGCTCAATCAGGTCAAGAGGTATCCACTGGTCCATCTGTTGACTCACGTAATTATGCAGGAAGTGAACGACCAAGTACTGGCTATGTGGATGTAGCTGGGCAGTCCAGTGTATCACTACCTGGTTGTACATCTGAAAGTGAAGGTCAGAAGGATTCAGCAAGTGGTAGTACATTGAAAAACGATTCAAGATCCCCAGCTGGGGGTCCTCTAAGCTCTTCAAGTGGCCAAAACACCGTGGTGAGAGAAGATGAGAAGGGAAGTGCTCCACAATCCAGTGAGAAGCAGGCTGAGGGTGCGAAAGCTGTTCCACTTGGTTTAGGACTTGGAGTTTTGGAGCGTAAGAGACAAGCCAGGCAGCCAAAACCATCGACCAAGAATGGAGGTGGCGGAACAACTAGTGCTCCCATCAATCAGAATCAGCAAGTAATAGGTGGCCAGCAAGTTTTGCAATCCCTTGCGTCTCGTGGTTCTGCTGCAAGTAGGATGAACTTAAATGATGCACCTGAAAGACAAACGGCCCCTGCTGTTGGGCAGGTCAGAGATGGTAGAACATTGGGAGGGCAAGGTCCTATTGGCCAAGTTGATATGGGAAGCATGATGTCTCAGGTTCTGCAGAGTCCAGCTCTGAATGGTCTGTTGGCAGGTGTTTCAGACCAAACTGGAGTGGGTTCTCCCGATGTATTGAGGAATATGCTACAGAGCTTTACACAGAATCCACAGATGAGGAGTGCTGTCAATCAGATTGCCGAACAGGTTGACGGCCAAGATTTGGGAAACTTGTTTGGAGGAGGCCAAGGTGGGGGGATCGATATGTCCAGGATGTTCCAACAAATGATGCCCATTGTAACTCGTGCCCTTGGGGTTGGATCGCCCCCCGTCCGGCCATCCTCTGCTTTGTCTCCTGAATCTCATCCGCCCCACAATGAGCGAAGTCTCAGTAGAGATGATAATATCCCTAGTTCTGAGGTTAACCTCGAAGAAGTGGTTCAAAGAATCGAGAACCTGAATGCACCAGGGGAAGTTTTTCATGCTTTAGTTGAAAATTCGGTGCAGCTGTCTGGCAGAGGAAGCGGTTCTCAAGAACTTGTGGATGAGTTGTGCAGGGACGAGAGTCTCTCCGGCGAATATGTTGAAATATTACGTAGGGACATACGGCGACGACTTGAGGGTGATTCGGGCAAGGACAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001822 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010605 GO:0010941 GO:0012501 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031593 GO:0031647 GO:0032268 GO:0032269 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042981 GO:0043021 GO:0043022 GO:0043067 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045861 GO:0045995 GO:0048232 GO:0048285 GO:0048513 GO:0048519 GO:0048523 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070059 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1903046 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

889

Amino Acids

92.44

Weight (kDa)

5.83

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 179
AarI CACCTGC 1 cut(s) 2105
Acc36I ACCTGC 2 cut(s) 1996, 2105
AccB1I GGYRCC 2 cut(s) 395, 1304
AccB7I CCANNNNNTGG 2 cut(s) 1550, 2242
AciI CCGC 6 cut(s) 91, 743, 1385, 1860, 2387, 2544
AclWI GGATC 4 cut(s) 245, 1656, 2285, 2347
AcoI YGGCCR 8 cut(s) 280, 985, 1273, 1696, 1906, 2047, 2233, 2354
AcsI RAATTY 3 cut(s) 918, 1441, 2515
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 5 cut(s) 214, 1137, 1549, 1600, 1642
AhlI ACTAGT 1 cut(s) 1868
AjnI CCWGG 9 cut(s) 399, 463, 1021, 1275, 1363, 1591, 1823, 2288, 2485
AjuI GAANNNNNNNTTGG 2 cut(s) 2093, 2125
AleI CACNNNNGTG 1 cut(s) 1709
AloI GAACNNNNNNTCC 2 cut(s) 746, 778
Alw21I GWGCWC 3 cut(s) 1186, 1738, 1876
Alw26I GTCTC 9 cut(s) 897, 1023, 1345, 1810, 1938, 2079, 2376, 2411, 2591
AlwI GGATC 4 cut(s) 245, 1656, 2285, 2347
AlwNI CAGNNNCTG 3 cut(s) 94, 1282, 2083
ApeKI GCWGC 9 cut(s) 88, 184, 599, 602, 605, 1282, 1828, 1946, 2524
ApoI RAATTY 3 cut(s) 918, 1441, 2515
Asp700I GAANNNNTTC 3 cut(s) 2457, 2496, 2545
AspA2I CCTAGG 1 cut(s) 746
AspLEI GCGC 1 cut(s) 371
AspS9I GGNCC 9 cut(s) 1046, 1073, 1090, 1163, 1361, 1502, 1676, 1990, 2039
AsuC2I CCSGG 1 cut(s) 1359
AsuHPI GGTGA 5 cut(s) 344, 457, 763, 1723, 2654
AvaII GGWCC 6 cut(s) 1090, 1163, 1361, 1502, 1676, 2039
AvrII CCTAGG 1 cut(s) 746
AxyI CCTNAGG 2 cut(s) 576, 789
BaeGI GKGCMC 2 cut(s) 162, 2329
BalI TGGCCA 5 cut(s) 282, 1275, 1698, 1908, 2049
BanI GGYRCC 2 cut(s) 395, 1304
BauI CACGAG 3 cut(s) 267, 1935, 2322
Bbv12I GWGCWC 3 cut(s) 1186, 1738, 1876
BbvCI CCTCAGC 1 cut(s) 1761
BbvI GCAGC 9 cut(s) 100, 196, 589, 611, 617, 1269, 1840, 1933, 2536
BccI CCATC 8 cut(s) 135, 631, 1324, 1513, 1846, 1886, 2009, 2365
BceAI ACGGC 4 cut(s) 172, 2004, 2248, 2642
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 9 cut(s) 401, 465, 1023, 1277, 1365, 1593, 1825, 2290, 2487
BciVI GTATCC 2 cut(s) 1256, 1504
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 1 cut(s) 1359
BcoDI GTCTC 9 cut(s) 897, 1023, 1345, 1810, 1938, 2079, 2376, 2411, 2591
BcuI ACTAGT 1 cut(s) 1868
BfaI CTAG 6 cut(s) 255, 747, 1224, 1260, 1869, 2430
BfmI CTRYAG 4 cut(s) 603, 768, 2084, 2168
BfuAI ACCTGC 2 cut(s) 1996, 2105
BfuI GTATCC 2 cut(s) 1256, 1504
BlnI CCTAGG 1 cut(s) 746
BmcAI AGTACT 1 cut(s) 1549
Bme18I GGWCC 6 cut(s) 1090, 1163, 1361, 1502, 1676, 2039
BmgT120I GGNCC 9 cut(s) 1046, 1073, 1090, 1163, 1361, 1502, 1676, 1990, 2039
BmiI GGNNCC 5 cut(s) 397, 1306, 1396, 1677, 1992
BmsI GCATC 4 cut(s) 768, 1358, 1960, 2298
BoxI GACNNNNGTC 1 cut(s) 2583
BplI GAGNNNNNCTC 4 cut(s) 2080, 2112, 2573, 2605
BpmI CTGGAG 2 cut(s) 1044, 2154
Bpu10I CCTNAGC 1 cut(s) 1761
BpuEI CTTGAG 3 cut(s) 998, 2535, 2657
BpuMI CCSGG 1 cut(s) 1359
Bsa29I ATCGAT 1 cut(s) 2280
BsaAI YACGTR 2 cut(s) 1520, 2615
BsaBI GATNNNNATC 2 cut(s) 126, 354
BsaJI CCNNGG 8 cut(s) 399, 514, 746, 1187, 1707, 2266, 2329, 2486
BsaXI ACNNNNNCTCC 4 cut(s) 1017, 1047, 1858, 1888
Bse1I ACTGG 9 cut(s) 77, 1286, 1308, 1333, 1504, 1555, 1578, 1746, 2137
Bse21I CCTNAGG 2 cut(s) 576, 789
Bse3DI GCAATG 4 cut(s) 164, 861, 957, 1459
Bse8I GATNNNNATC 2 cut(s) 126, 354
BseBI CCWGG 9 cut(s) 401, 465, 1023, 1277, 1365, 1593, 1825, 2290, 2487
BseCI ATCGAT 1 cut(s) 2280
BseDI CCNNGG 8 cut(s) 399, 514, 746, 1187, 1707, 2266, 2329, 2486
BseJI GATNNNNATC 2 cut(s) 126, 354
BseMI GCAATG 4 cut(s) 164, 861, 957, 1459
BseMII CTCAG 8 cut(s) 129, 590, 780, 844, 1752, 2090, 2422, 2427
BseNI ACTGG 9 cut(s) 77, 1286, 1308, 1333, 1504, 1555, 1578, 1746, 2137
BseRI GAGGAG 6 cut(s) 531, 582, 1333, 1387, 2212, 2274
BseSI GKGCMC 2 cut(s) 162, 2329
BseXI GCAGC 9 cut(s) 100, 196, 589, 611, 617, 1269, 1840, 1933, 2536
BseYI CCCAGC 6 cut(s) 192, 1043, 1066, 1568, 1666, 1670
BsgI GTGCAG 3 cut(s) 834, 2543, 2593
BshNI GGYRCC 2 cut(s) 395, 1304
BshVI ATCGAT 1 cut(s) 2280
BsiHKAI GWGCWC 3 cut(s) 1186, 1738, 1876
BsiSI CCGG 3 cut(s) 1359, 2353, 2592
BslFI GGGAC 4 cut(s) 175, 322, 2591, 2633
BsmAI GTCTC 9 cut(s) 897, 1023, 1345, 1810, 1938, 2079, 2376, 2411, 2591
BsmBI CGTCTC 1 cut(s) 1938
BsmFI GGGAC 4 cut(s) 175, 322, 2591, 2633
BsmI GAATGC 2 cut(s) 1045, 2485
Bsp1286I GDGCHC 5 cut(s) 162, 1186, 1738, 1876, 2329
Bsp1407I TGTACA 1 cut(s) 1598
Bsp143I GATC 8 cut(s) 61, 250, 325, 349, 835, 1661, 2277, 2339
BspACI CCGC 6 cut(s) 91, 743, 1385, 1860, 2387, 2544
BspCNI CTCAG 8 cut(s) 130, 589, 781, 843, 1753, 2089, 2421, 2428
BspDI ATCGAT 1 cut(s) 2280
BspLI GGNNCC 5 cut(s) 397, 1306, 1396, 1677, 1992
BspMAI CTGCAG 3 cut(s) 607, 772, 2088
BspMI ACCTGC 2 cut(s) 1996, 2105
BspPI GGATC 4 cut(s) 245, 1656, 2285, 2347
BspQI GCTCTTC 1 cut(s) 1693
BspT107I GGYRCC 2 cut(s) 395, 1304
BsrDI GCAATG 4 cut(s) 164, 861, 957, 1459
BsrGI TGTACA 1 cut(s) 1598
BsrI ACTGG 9 cut(s) 77, 1286, 1308, 1333, 1504, 1555, 1578, 1746, 2137
BssECI CCNNGG 8 cut(s) 399, 514, 746, 1187, 1707, 2266, 2329, 2486
BssMI GATC 8 cut(s) 61, 250, 325, 349, 835, 1661, 2277, 2339
BssSI CACGAG 3 cut(s) 267, 1935, 2322
BssT1I CCWWGG 4 cut(s) 746, 1187, 2266, 2329
Bst2BI CACGAG 3 cut(s) 267, 1935, 2322
Bst2UI CCWGG 9 cut(s) 401, 465, 1023, 1277, 1365, 1593, 1825, 2290, 2487
Bst4CI ACNGT 3 cut(s) 296, 534, 1708
Bst6I CTCTTC 4 cut(s) 103, 975, 1110, 1693
BstAPI GCANNNNNTGC 2 cut(s) 166, 1918
BstAUI TGTACA 1 cut(s) 1598
BstBAI YACGTR 2 cut(s) 1520, 2615
BstC8I GCNNGC 4 cut(s) 948, 1050, 1758, 1910
BstDSI CCRYGG 1 cut(s) 1707
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 8 cut(s) 64, 253, 328, 352, 838, 1664, 2280, 2342
BstMAI GTCTC 9 cut(s) 897, 1023, 1345, 1810, 1938, 2079, 2376, 2411, 2591
BstMBI GATC 8 cut(s) 61, 250, 325, 349, 835, 1661, 2277, 2339
BstNI CCWGG 9 cut(s) 401, 465, 1023, 1277, 1365, 1593, 1825, 2290, 2487
BstNSI RCATGY 2 cut(s) 236, 1214
BstPAI GACNNNNGTC 1 cut(s) 2583
BstSFI CTRYAG 4 cut(s) 603, 768, 2084, 2168
BstSLI GKGCMC 2 cut(s) 162, 2329
BstSNI TACGTA 1 cut(s) 2615
BstV1I GCAGC 9 cut(s) 100, 196, 589, 611, 617, 1269, 1840, 1933, 2536
BstX2I RGATCY 1 cut(s) 1661
BstYI RGATCY 1 cut(s) 1661
Bsu15I ATCGAT 1 cut(s) 2280
Bsu36I CCTNAGG 2 cut(s) 576, 789
BsuI GTATCC 2 cut(s) 1256, 1504
BsuTUI ATCGAT 1 cut(s) 2280
BtgI CCRYGG 1 cut(s) 1707
BtgZI GCGATG 1 cut(s) 1335
BtsI GCAGTG 1 cut(s) 765
BtsIMutI CAGTG 6 cut(s) 301, 765, 1293, 1497, 1585, 1753
BveI ACCTGC 2 cut(s) 1996, 2105
Cac8I GCNNGC 4 cut(s) 948, 1050, 1758, 1910
CaiI CAGNNNCTG 3 cut(s) 94, 1282, 2083
CfoI GCGC 1 cut(s) 371
Cfr13I GGNCC 9 cut(s) 1046, 1073, 1090, 1163, 1361, 1502, 1676, 1990, 2039
ClaI ATCGAT 1 cut(s) 2280
CseI GACGC 3 cut(s) 879, 1009, 1920
CsiI ACCWGGT 1 cut(s) 1591
Csp6I GTAC 5 cut(s) 213, 1136, 1548, 1599, 1641
CspCI CAANNNNNGTGG 2 cut(s) 630, 665
CviAII CATG 8 cut(s) 233, 596, 616, 815, 839, 1211, 2068, 2501
CviQI GTAC 5 cut(s) 213, 1136, 1548, 1599, 1641
DpnI GATC 8 cut(s) 63, 252, 327, 351, 837, 1663, 2279, 2341
DpnII GATC 8 cut(s) 61, 250, 325, 349, 835, 1661, 2277, 2339
DraI TTTAAA 1 cut(s) 456
DraIII CACNNNGTG 1 cut(s) 296
EaeI YGGCCR 8 cut(s) 280, 985, 1273, 1696, 1906, 2047, 2233, 2354
Eam1104I CTCTTC 4 cut(s) 103, 975, 1110, 1693
EarI CTCTTC 4 cut(s) 103, 975, 1110, 1693
EciI GGCGGA 2 cut(s) 1875, 2376
Eco105I TACGTA 1 cut(s) 2615
Eco130I CCWWGG 4 cut(s) 746, 1187, 2266, 2329
Eco32I GATATC 1 cut(s) 288
Eco47I GGWCC 6 cut(s) 1090, 1163, 1361, 1502, 1676, 2039
Eco81I CCTNAGG 2 cut(s) 576, 789
EcoO109I RGGNCCY 3 cut(s) 1073, 1676, 2039
EcoRII CCWGG 9 cut(s) 399, 463, 1021, 1275, 1363, 1591, 1823, 2288, 2485
EcoRV GATATC 1 cut(s) 288
EcoT14I CCWWGG 4 cut(s) 746, 1187, 2266, 2329
ErhI CCWWGG 4 cut(s) 746, 1187, 2266, 2329
Esp3I CGTCTC 1 cut(s) 1938
FaeI CATG 8 cut(s) 236, 599, 619, 818, 842, 1214, 2071, 2504
FaqI GGGAC 4 cut(s) 175, 322, 2591, 2633
FatI CATG 8 cut(s) 232, 595, 615, 814, 838, 1210, 2067, 2500
FbaI TGATCA 1 cut(s) 61
FspBI CTAG 6 cut(s) 255, 747, 1224, 1260, 1869, 2430
GlaI GCGC 1 cut(s) 370
GsaI CCCAGC 6 cut(s) 196, 1047, 1070, 1572, 1670, 1674
GsuI CTGGAG 2 cut(s) 1044, 2154
HapII CCGG 3 cut(s) 1359, 2353, 2592
HgaI GACGC 3 cut(s) 879, 1009, 1920
HhaI GCGC 1 cut(s) 371
Hin1II CATG 8 cut(s) 236, 599, 619, 818, 842, 1214, 2071, 2504
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HincII GTYRAC 4 cut(s) 511, 1513, 2230, 2443
HindII GTYRAC 4 cut(s) 511, 1513, 2230, 2443
HpaI GTTAAC 1 cut(s) 2443
HpaII CCGG 3 cut(s) 1359, 2353, 2592
HphI GGTGA 5 cut(s) 344, 457, 763, 1723, 2654
Hpy166II GTNNAC 7 cut(s) 511, 754, 1198, 1513, 1538, 2230, 2443
Hpy8I GTNNAC 7 cut(s) 511, 754, 1198, 1513, 1538, 2230, 2443
Hpy99I CGWCG 2 cut(s) 211, 2634
HpyAV CCTTC 8 cut(s) 213, 329, 488, 771, 1398, 1607, 1615, 1720
HpyCH4III ACNGT 3 cut(s) 296, 534, 1708
HpyCH4IV ACGT 3 cut(s) 311, 1519, 2614
HpySE526I ACGT 3 cut(s) 311, 1519, 2614
Hsp92II CATG 8 cut(s) 236, 599, 619, 818, 842, 1214, 2071, 2504
HspAI GCGC 1 cut(s) 369
Ksp22I TGATCA 1 cut(s) 61
KspAI GTTAAC 1 cut(s) 2443
Kzo9I GATC 8 cut(s) 61, 250, 325, 349, 835, 1661, 2277, 2339
LguI GCTCTTC 1 cut(s) 1693
LmnI GCTCC 6 cut(s) 682, 1189, 1400, 1741, 1807, 1879
Lsp1109I GCAGC 9 cut(s) 100, 196, 589, 611, 617, 1269, 1840, 1933, 2536
LweI GCATC 4 cut(s) 768, 1358, 1960, 2298
MabI ACCWGGT 1 cut(s) 1591
MaeI CTAG 6 cut(s) 255, 747, 1224, 1260, 1869, 2430
MaeII ACGT 3 cut(s) 311, 1519, 2614
MaeIII GTNAC 1 cut(s) 2317
MalI GATC 8 cut(s) 63, 252, 327, 351, 837, 1663, 2279, 2341
MboI GATC 8 cut(s) 61, 250, 325, 349, 835, 1661, 2277, 2339
MboII GAAGA 8 cut(s) 120, 800, 962, 965, 1097, 1680, 1730, 2462
MflI RGATCY 1 cut(s) 1661
MhlI GDGCHC 5 cut(s) 162, 1186, 1738, 1876, 2329
MlsI TGGCCA 5 cut(s) 282, 1275, 1698, 1908, 2049
MluNI TGGCCA 5 cut(s) 282, 1275, 1698, 1908, 2049
MlyI GAGTC 3 cut(s) 1508, 2098, 2593
MmeI TCCRAC 3 cut(s) 301, 2317, 2323
Mox20I TGGCCA 5 cut(s) 282, 1275, 1698, 1908, 2049
MroXI GAANNNNTTC 3 cut(s) 2457, 2496, 2545
MscI TGGCCA 5 cut(s) 282, 1275, 1698, 1908, 2049
MseI TTAA 7 cut(s) 116, 315, 354, 455, 1425, 1964, 2442
MslI CAYNNNNRTG 2 cut(s) 1209, 1709
Msp20I TGGCCA 5 cut(s) 282, 1275, 1698, 1908, 2049
MspA1I CMGCKG 4 cut(s) 91, 602, 1670, 2527
MspI CCGG 3 cut(s) 1359, 2353, 2592
Mva1269I GAATGC 2 cut(s) 1045, 2485
MvaI CCWGG 9 cut(s) 401, 465, 1023, 1277, 1365, 1593, 1825, 2290, 2487
NciI CCSGG 1 cut(s) 1359
NdeII GATC 8 cut(s) 61, 250, 325, 349, 835, 1661, 2277, 2339
NlaIII CATG 8 cut(s) 236, 599, 619, 818, 842, 1214, 2071, 2504
NlaIV GGNNCC 5 cut(s) 397, 1306, 1396, 1677, 1992
NspI RCATGY 2 cut(s) 236, 1214
OliI CACNNNNGTG 1 cut(s) 1709
PaqCI CACCTGC 1 cut(s) 2105
PciSI GCTCTTC 1 cut(s) 1693
PctI GAATGC 2 cut(s) 1045, 2485
PdmI GAANNNNTTC 3 cut(s) 2457, 2496, 2545
PflMI CCANNNNNTGG 2 cut(s) 1550, 2242
PfoI TCCNGGA 2 cut(s) 1021, 2288
PleI GAGTC 3 cut(s) 1508, 2097, 2592
PpsI GAGTC 3 cut(s) 1508, 2097, 2592
Ppu21I YACGTR 2 cut(s) 1520, 2615
PpuMI RGGWCCY 2 cut(s) 1676, 2039
PshAI GACNNNNGTC 1 cut(s) 2583
PsiI TTATAA 1 cut(s) 179
Psp5II RGGWCCY 2 cut(s) 1676, 2039
Psp6I CCWGG 9 cut(s) 399, 463, 1021, 1275, 1363, 1591, 1823, 2288, 2485
PspFI CCCAGC 6 cut(s) 192, 1043, 1066, 1568, 1666, 1670
PspGI CCWGG 9 cut(s) 399, 463, 1021, 1275, 1363, 1591, 1823, 2288, 2485
PspN4I GGNNCC 5 cut(s) 397, 1306, 1396, 1677, 1992
PspPI GGNCC 9 cut(s) 1046, 1073, 1090, 1163, 1361, 1502, 1676, 1990, 2039
PspPPI RGGWCCY 2 cut(s) 1676, 2039
PstI CTGCAG 3 cut(s) 607, 772, 2088
PstNI CAGNNNCTG 3 cut(s) 94, 1282, 2083
PsuI RGATCY 1 cut(s) 1661
PvuII CAGCTG 3 cut(s) 602, 1670, 2527
RsaI GTAC 5 cut(s) 214, 1137, 1549, 1600, 1642
RsaNI GTAC 5 cut(s) 213, 1136, 1548, 1599, 1641
RseI CAYNNNNRTG 2 cut(s) 1209, 1709
SapI GCTCTTC 1 cut(s) 1693
SaqAI TTAA 7 cut(s) 116, 315, 354, 455, 1425, 1964, 2442
Sau3AI GATC 8 cut(s) 61, 250, 325, 349, 835, 1661, 2277, 2339
Sau96I GGNCC 9 cut(s) 1046, 1073, 1090, 1163, 1361, 1502, 1676, 1990, 2039
ScaI AGTACT 1 cut(s) 1549
SchI GAGTC 3 cut(s) 1508, 2098, 2593
SduI GDGCHC 5 cut(s) 162, 1186, 1738, 1876, 2329
SexAI ACCWGGT 1 cut(s) 1591
SfaNI GCATC 4 cut(s) 768, 1358, 1960, 2298
SfcI CTRYAG 4 cut(s) 603, 768, 2084, 2168
SinI GGWCC 6 cut(s) 1090, 1163, 1361, 1502, 1676, 2039
SmiMI CAYNNNNRTG 2 cut(s) 1209, 1709
SmlI CTYRAG 3 cut(s) 977, 2550, 2636
SmoI CTYRAG 3 cut(s) 977, 2550, 2636
SnaBI TACGTA 1 cut(s) 2615
SpeI ACTAGT 1 cut(s) 1868
SsiI CCGC 6 cut(s) 91, 743, 1385, 1860, 2387, 2544
SspI AATATT 1 cut(s) 2610
SspMI CTAG 6 cut(s) 255, 747, 1224, 1260, 1869, 2430
StyI CCWWGG 4 cut(s) 746, 1187, 2266, 2329
TaaI ACNGT 3 cut(s) 296, 534, 1708
TaiI ACGT 3 cut(s) 314, 1522, 2617
TaqI TCGA 4 cut(s) 1841, 2280, 2448, 2469
TatI WGTACW 3 cut(s) 1547, 1598, 1640
TauI GCSGC 2 cut(s) 94, 1387
Tru1I TTAA 7 cut(s) 116, 315, 354, 455, 1425, 1964, 2442
Tru9I TTAA 7 cut(s) 116, 315, 354, 455, 1425, 1964, 2442
TscAI CASTG 6 cut(s) 301, 772, 1293, 1504, 1585, 1753
TseI GCWGC 9 cut(s) 88, 184, 599, 602, 605, 1282, 1828, 1946, 2524
TspDTI ATGAA 6 cut(s) 27, 501, 686, 1434, 1973, 2489
TspRI CASTG 6 cut(s) 301, 772, 1293, 1504, 1585, 1753
Van91I CCANNNNNTGG 2 cut(s) 1550, 2242
VpaK11BI GGWCC 6 cut(s) 1090, 1163, 1361, 1502, 1676, 2039
XapI RAATTY 3 cut(s) 918, 1441, 2515
XceI RCATGY 2 cut(s) 236, 1214
XmaJI CCTAGG 1 cut(s) 746
XmnI GAANNNNTTC 3 cut(s) 2457, 2496, 2545
XspI CTAG 6 cut(s) 255, 747, 1224, 1260, 1869, 2430
ZrmI AGTACT 1 cut(s) 1549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.