Prupe.1G356800_v2.0.a1

Large proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0005387
Physical Location & Seq
Forward (+)
33050338 .. 33058285
7948 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G356800.2

Sequence Viewer

Length: 2838 bp
ATGGCAAATCAGCACTCCAGTGAAGGCTCTAGTTCTGGCAACGTTGCCGGGGAAAGTTCTGATTCAACTGTTGAGATCAATATTAAGACTTTAGACTCACAGATATATAGTTTTCAAGTGGAGAAAAATATGCCAGTTTCATTGTTCAAGGAAAAAATAGCTAATCAAATAGGTGTTCCAGTTGGTCAGCAGCGGCTGATTTTCAGGGGAAAGGTGCTAAAGGATGATCATCCTCTCTCTGAGTATCATTTGGAAAATGGGCATACATTGCACTTAGTTATAAGGCAACCATCCCAGTCACAACCTTCATCTGGGACAAGTTCTGGCGACCCACATGTGAATAATGGAAATGAGGCTAGTGGTGTTCCTCGTGGTCGCATTGGACAGATATCACACAGCGTAGTCCTTGGGACATTTAATGTTGGAGACCAAGGTGAAGGCATTGTTCCTGACCTTAGTCGGGTTATTGGTGCAGTTTTGAATTCTATTGGAGTCGGCACCCAGGCTACAACTAATGTTACAGGCAACGTACAGTCCACTACTTCGTCAAACAATTCTGGCCAGTCTCCTCACGGAAATGATGCAGAAGGGTCGCATAATGTCATTGGTCCGAACCGAGGAGGAAATCAAGCACAGTCTGGACAGGCATTTCCTGCTCAACCATTCCAAACTTTTCCTCAATCTGTGCAAAGTCCTGCAGCTGCAGCATTTCCAATTCCTTCGTTGAATATGCCAATTCCAGACTCTTTGAATACGCTCTCTGAGTTTATGAACAGAATGGAGCAGGCATTGACACAAAATGGTTACCAGCCAAATTTATCTGCAACAAACACAGGAGACGTGCCTAGGGTGAATTTACCTTCCAATGCACAGGGGATGCCCACACCTGAAGCATTGGGCATTGTTCTTCGTCATGCAGAGCGACTTCTAAGCAGTCATGCTGTTTCTGCACTATCTCACATTGCAGGACGCTTGGAGCAAGAAGGAGCATCTTCAGATCCCTCTGTGAGGGGCCAAATTCAGACAGAATTTATGCAAGTAGGGCTTGCAATGCAACATTTAGGTGCTCTTTTTCTTGAGCTTGGCCGGACAATTTGGACACTTCGTATGGGACAGTCTCCTGGAGAAGCTGTTGTGAATGCTGGGCCAGCAGTTTATATTTCCCCATCAGGGCCTAATCCCATAATGGTCCAGCCTTTTCCTCTTCAAACAAGCACAATGCTTGGTGGTTCTGTACCTCAATCAAATCCTATGACTTTTGGTCCTGTTGGTGTTGGAAGTGCTCCAAGGAATGTAAACATTCATATACATGCTGGTACCTCACTTGCGCCAATTGTTTCTGCAGTTGGTGGTGCTAGGGGAAGTAATGGGGAGGGTATGCCAAGAGAACATCGTAATGGGTCTGTGTCTAGAGATTCAGGTGCACGGGTGCTGCCAGTGAGAAATGTAATTGCTGGCACTATGCCAACATCCCAAACTGGTATTGCAATCTCTGGTGTGTCACAACCAGGTCTTTCTGCTTCACAGCCGCCTTCCGACTCCTCACTTTCCTCCATTGTTTCTGAACTTAATTCACATATTAGAAATTTGGTTGGCAATATGCAAGGAGAAGACGCAGTTCAATCAGGTCAAGAGGTACCCAATGTTCAGAGTTCATCTGTTGGCTTTGAATTGCGTAATGATGCAGGAAGTGAACGGCCCAGTACTGCATTTGTTGATGGAGCTGGGCAATCTAGTGTGCTGCTACCTGGTTGCACATCTGAAGGTGGAGGTCAGAAGGATTCAGGAAGTGTTCCTACCTTGAAAGATGATTCAAGATTCCCAGCTGGGGGTCCTCTAAGCTCTTCAAGTGGACAAAATACCTTGGCGAGAAAAGATGAGAAAGAAAGTGGTCCACAATCCAGTGAGAAGCATGATATGACTGAGGGTACAAAAGCTGTTCCACTTGGATTAGGACTTGGAGTTTTGGACCGTAAGAGACAAGGCAGGCAGCAAAAACCACCAGCCAAGAATGGTGATGGCGGAATGGCTAGTGCTCCCATCAATCAGAATCAGCAAGTAACAGGTGGTCAGCAAGTTTTGCAGTCTCTTGCATCTCGTGGTTCTGCTGTAAGTAGGATGAACACAAGTGATGAGCCTGCAAGACAAACAGCCCCTGCTATTGGCCAGGTCAGAGATGGTAGAACATTGGGAGCACAAGGTCCTGTTGACCAAGTTGATATGGGAAGCGTGATGTCTCAGGTTCTGCGTAGTCCTGCCCTGAATGGTTTGTTGGCAGGTGTTTCAGAGCAAACTGGAGTGGGTTCTCCTGATGCATTGAGGAATATGTTGCAGAGCTTTACACAGAGTCCACAGATGAGGAATGCTGTCAATCAGATTGTTGAACAGGTTGACAGCCAAGATGTTGGAAATATGTTTGCAGGACTTGGAGGAGGCCAAGGTGGCGGGATTGACATGTCCAGGATGTTCCAACAAATGATGCCAATTGTATCTCGAGCCCTTGGGGCTGGGTCCACTCTCGGCCAACCAAACCCTGTTTTGGAACCTGAATCTCATCAACCATACAATGAGAGAAGTCTTCGCAGAGATGATAATGTCCCAAATCCTGAGATTAACCTGCAAGAAGTGGTTCAGAGGATTGGGAACCTGAATGCACCAGGAGATGTTTTTCACGCTGTAGTTGAAAATTCAGTTGAGCTGTCTGGCAGAGGAAGCGGTCCACAAGAACTTGTGGATGAGTTGTGCAGGGACGATGGTCTCTCTAGAGAATACGTTGAGATATTACGAAGGGACATACGTAGACGCCTTGAGGGTAATTCGGGGCAGGACAAGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001822 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010605 GO:0010941 GO:0012501 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031593 GO:0031647 GO:0032268 GO:0032269 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042981 GO:0043021 GO:0043022 GO:0043067 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045861 GO:0045995 GO:0048232 GO:0048285 GO:0048513 GO:0048519 GO:0048523 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070059 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1903046 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

946

Amino Acids

98.81

Weight (kDa)

5.67

Isoelectric Point (pI)

54.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 281
AarI CACCTGC 1 cut(s) 2267
Acc36I ACCTGC 2 cut(s) 2267, 2625
Acc65I GGTACC 2 cut(s) 1316, 1636
AccB1I GGYRCC 3 cut(s) 497, 1316, 1636
AccI GTMKAC 1 cut(s) 2800
AciI CCGC 5 cut(s) 193, 1529, 2022, 2445, 2715
AclI AACGTT 1 cut(s) 42
AclWI GGATC 1 cut(s) 992
AcoI YGGCCR 4 cut(s) 559, 1084, 2164, 2521
AcsI RAATTY 7 cut(s) 481, 814, 853, 1017, 1028, 1585, 2686
AcuI CTGAAG 3 cut(s) 909, 978, 1782
AcyI GRCGYC 1 cut(s) 2803
AfaI GTAC 6 cut(s) 531, 1236, 1318, 1638, 1705, 1930
AfiI CCNNNNNNNGG 9 cut(s) 311, 460, 617, 1008, 1170, 1828, 1829, 2162, 2539
AflIII ACRYGT 2 cut(s) 334, 2454
AhdI GACNNNNNGTC 1 cut(s) 1260
AjiI CACGTC 1 cut(s) 841
AjnI CCWGG 7 cut(s) 501, 1120, 1507, 1747, 2166, 2459, 2656
AjuI GAANNNNNNNTTGG 2 cut(s) 2255, 2287
AleI CACNNNNGTG 1 cut(s) 18
Alw21I GWGCWC 5 cut(s) 1069, 1285, 1426, 2038, 2197
Alw26I GTCTC 8 cut(s) 420, 570, 831, 1122, 1972, 2091, 2241, 2762
Alw44I GTGCAC 1 cut(s) 1422
AlwI GGATC 1 cut(s) 992
AlwNI CAGNNNCTG 3 cut(s) 196, 2156, 2245
Ama87I CYCGRG 1 cut(s) 2493
AoxI GGCC 9 cut(s) 559, 1012, 1084, 1145, 1172, 1697, 2164, 2434, 2521
ApaLI GTGCAC 1 cut(s) 1422
ApeKI GCWGC 7 cut(s) 190, 698, 701, 704, 1432, 1741, 1990
ApoI RAATTY 7 cut(s) 481, 814, 853, 1017, 1028, 1585, 2686
Asp700I GAANNNNTTC 1 cut(s) 2628
Asp718I GGTACC 2 cut(s) 1316, 1636
AspA2I CCTAGG 1 cut(s) 845
AspLEI GCGC 1 cut(s) 1330
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 3 cut(s) 446, 862, 2027
AvaI CYCGRG 1 cut(s) 2493
AvaII GGWCC 9 cut(s) 608, 1189, 1262, 1832, 1892, 1969, 2201, 2511, 2717
AvrII CCTAGG 1 cut(s) 845
BaeGI GKGCMC 1 cut(s) 1426
BalI TGGCCA 2 cut(s) 561, 2166
BanI GGYRCC 3 cut(s) 497, 1316, 1636
BanII GRGCYC 1 cut(s) 2500
BarI GAAGNNNNNNTAC 2 cut(s) 1780, 1812
BauI CACGAG 2 cut(s) 369, 2097
BbsI GAAGAC 2 cut(s) 1617, 2570
Bbv12I GWGCWC 5 cut(s) 1069, 1285, 1426, 2038, 2197
BbvI GCAGC 7 cut(s) 202, 688, 710, 716, 1419, 1728, 2002
BccI CCATC 7 cut(s) 298, 1174, 1712, 2012, 2048, 2171, 2747
BceAI ACGGC 1 cut(s) 1712
BcgI CGANNNNNNTGC 2 cut(s) 573, 607
BciT130I CCWGG 7 cut(s) 503, 1122, 1509, 1749, 2168, 2461, 2658
BclI TGATCA 1 cut(s) 226
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 8 cut(s) 420, 570, 831, 1122, 1972, 2091, 2241, 2762
BfaI CTAG 8 cut(s) 30, 357, 846, 1356, 1410, 1734, 2031, 2763
BfmI CTRYAG 4 cut(s) 696, 702, 1341, 2676
BfuAI ACCTGC 2 cut(s) 2267, 2625
BglI GCCNNNNNGGC 2 cut(s) 2442, 2504
BisI GCNGC 9 cut(s) 191, 194, 699, 702, 705, 1433, 1529, 1742, 1991
BlnI CCTAGG 1 cut(s) 845
BlsI GCNGC 9 cut(s) 192, 195, 700, 703, 706, 1434, 1530, 1743, 1992
BmcAI AGTACT 1 cut(s) 1705
Bme1390I CCNGG 8 cut(s) 49, 503, 1122, 1509, 1749, 2168, 2461, 2658
Bme18I GGWCC 9 cut(s) 608, 1189, 1262, 1832, 1892, 1969, 2201, 2511, 2717
BmeRI GACNNNNNGTC 1 cut(s) 1260
BmeT110I CYCGRG 1 cut(s) 2493
BmgBI CACGTC 1 cut(s) 841
BmiI GGNNCC 8 cut(s) 499, 1013, 1318, 1638, 1833, 2512, 2544, 2645
BmrFI CCNGG 8 cut(s) 49, 503, 1122, 1509, 1749, 2168, 2461, 2658
BmrI ACTGGG 2 cut(s) 289, 1695
BmsI GCATC 7 cut(s) 571, 867, 998, 1672, 2102, 2302, 2469
BmuI ACTGGG 2 cut(s) 289, 1695
BoxI GACNNNNGTC 2 cut(s) 456, 2754
BpiI GAAGAC 2 cut(s) 1617, 2570
BpmI CTGGAG 2 cut(s) 1143, 2316
BpuEI CTTGAG 2 cut(s) 1097, 2828
BpuMI CCSGG 1 cut(s) 49
BsaAI YACGTR 1 cut(s) 2798
BsaBI GATNNNNATC 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 2803
BsaI GGTCTC 2 cut(s) 420, 2762
BsaXI ACNNNNNCTCC 2 cut(s) 1116, 1146
Bsc4I CCNNNNNNNGG 9 cut(s) 311, 460, 617, 1008, 1170, 1828, 1829, 2162, 2539
Bse3DI GCAATG 3 cut(s) 266, 960, 1056
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 7 cut(s) 503, 1122, 1509, 1749, 2168, 2461, 2658
BseGI GGATG 8 cut(s) 229, 229, 290, 882, 1469, 2124, 2469, 2740
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 9 cut(s) 311, 460, 617, 1008, 1170, 1828, 1829, 2162, 2539
BseMI GCAATG 3 cut(s) 266, 960, 1056
BseMII CTCAG 5 cut(s) 231, 753, 1914, 2252, 2598
BseRI GAGGAG 4 cut(s) 558, 633, 1531, 2445
BseSI GKGCMC 1 cut(s) 1426
BseXI GCAGC 7 cut(s) 202, 688, 710, 716, 1419, 1728, 2002
BseYI CCCAGC 5 cut(s) 1142, 1724, 1822, 1826, 2507
BsgI GTGCAG 3 cut(s) 492, 933, 2764
BshFI GGCC 9 cut(s) 561, 1014, 1086, 1147, 1174, 1699, 2166, 2436, 2523
BshNI GGYRCC 3 cut(s) 497, 1316, 1636
BsiHKAI GWGCWC 5 cut(s) 1069, 1285, 1426, 2038, 2197
BsiHKCI CYCGRG 1 cut(s) 2493
BsiSI CCGG 2 cut(s) 48, 1087
BslFI GGGAC 6 cut(s) 328, 424, 1125, 2582, 2762, 2804
BslI CCNNNNNNNGG 9 cut(s) 311, 460, 617, 1008, 1170, 1828, 1829, 2162, 2539
BsmAI GTCTC 8 cut(s) 420, 570, 831, 1122, 1972, 2091, 2241, 2762
BsmBI CGTCTC 1 cut(s) 831
BsmFI GGGAC 6 cut(s) 328, 424, 1125, 2582, 2762, 2804
BsmI GAATGC 3 cut(s) 1144, 2368, 2656
BsnI GGCC 9 cut(s) 561, 1014, 1086, 1147, 1174, 1699, 2166, 2436, 2523
Bso31I GGTCTC 2 cut(s) 420, 2762
BsoBI CYCGRG 1 cut(s) 2493
Bsp1286I GDGCHC 6 cut(s) 1069, 1285, 1426, 2038, 2197, 2500
Bsp143I GATC 3 cut(s) 75, 226, 997
BspACI CCGC 5 cut(s) 193, 1529, 2022, 2445, 2715
BspANI GGCC 9 cut(s) 561, 1014, 1086, 1147, 1174, 1699, 2166, 2436, 2523
BspCNI CTCAG 5 cut(s) 232, 754, 1915, 2251, 2599
BspLI GGNNCC 8 cut(s) 499, 1013, 1318, 1638, 1833, 2512, 2544, 2645
BspMAI CTGCAG 3 cut(s) 700, 706, 1345
BspMI ACCTGC 2 cut(s) 2267, 2625
BspPI GGATC 1 cut(s) 992
BspQI GCTCTTC 1 cut(s) 1849
BspT107I GGYRCC 3 cut(s) 497, 1316, 1636
BspTNI GGTCTC 2 cut(s) 420, 2762
BsrDI GCAATG 3 cut(s) 266, 960, 1056
BssMI GATC 3 cut(s) 75, 226, 997
BssNI GRCGYC 1 cut(s) 2803
BssSI CACGAG 2 cut(s) 369, 2097
BssT1I CCWWGG 7 cut(s) 406, 430, 845, 1286, 1863, 2437, 2500
Bst2BI CACGAG 2 cut(s) 369, 2097
Bst2UI CCWGG 7 cut(s) 503, 1122, 1509, 1749, 2168, 2461, 2658
Bst4CI ACNGT 5 cut(s) 70, 534, 636, 1116, 1973
Bst6I CTCTTC 2 cut(s) 1209, 1849
BstACI GRCGYC 1 cut(s) 2803
BstAPI GCANNNNNTGC 3 cut(s) 268, 653, 2080
BstBAI YACGTR 1 cut(s) 2798
BstC8I GCNNGC 6 cut(s) 786, 1047, 1149, 1456, 1988, 2139
BstDEI CTNAG 9 cut(s) 240, 274, 455, 762, 929, 1838, 1923, 2238, 2607
BstEII GGTNACC 1 cut(s) 803
BstENI CCTNNNNNAGG 1 cut(s) 1006
BstF5I GGATG 8 cut(s) 229, 229, 290, 882, 1469, 2124, 2469, 2740
BstHHI GCGC 1 cut(s) 1330
BstKTI GATC 3 cut(s) 78, 229, 1000
BstMAI GTCTC 8 cut(s) 420, 570, 831, 1122, 1972, 2091, 2241, 2762
BstMBI GATC 3 cut(s) 75, 226, 997
BstNI CCWGG 7 cut(s) 503, 1122, 1509, 1749, 2168, 2461, 2658
BstNSI RCATGY 3 cut(s) 338, 1313, 2458
BstPAI GACNNNNGTC 2 cut(s) 456, 2754
BstPI GGTNACC 1 cut(s) 803
BstSCI CCNGG 8 cut(s) 47, 501, 1120, 1507, 1747, 2166, 2459, 2656
BstSFI CTRYAG 4 cut(s) 696, 702, 1341, 2676
BstSLI GKGCMC 1 cut(s) 1426
BstSNI TACGTA 1 cut(s) 2798
BstV1I GCAGC 7 cut(s) 202, 688, 710, 716, 1419, 1728, 2002
BstV2I GAAGAC 2 cut(s) 1617, 2570
BstX2I RGATCY 1 cut(s) 997
BstXI CCANNNNNNTGG 1 cut(s) 2405
BstYI RGATCY 1 cut(s) 997
BsuRI GGCC 9 cut(s) 561, 1014, 1086, 1147, 1174, 1699, 2166, 2436, 2523
BtrI CACGTC 1 cut(s) 841
BtsCI GGATG 8 cut(s) 229, 229, 290, 882, 1469, 2124, 2469, 2740
BtsIMutI CAGTG 3 cut(s) 25, 1443, 1909
BveI ACCTGC 2 cut(s) 2267, 2625
Cac8I GCNNGC 6 cut(s) 786, 1047, 1149, 1456, 1988, 2139
CaiI CAGNNNCTG 3 cut(s) 196, 2156, 2245
CfoI GCGC 1 cut(s) 1330
CseI GACGC 3 cut(s) 978, 1622, 2811
CsiI ACCWGGT 2 cut(s) 1507, 1747
Csp6I GTAC 6 cut(s) 530, 1235, 1317, 1637, 1704, 1929
CviAII CATG 6 cut(s) 335, 914, 938, 1310, 1913, 2455
CviQI GTAC 6 cut(s) 530, 1235, 1317, 1637, 1704, 1929
DdeI CTNAG 9 cut(s) 240, 274, 455, 762, 929, 1838, 1923, 2238, 2607
DpnI GATC 3 cut(s) 77, 228, 999
DpnII GATC 3 cut(s) 75, 226, 997
DriI GACNNNNNGTC 1 cut(s) 1260
EaeI YGGCCR 4 cut(s) 559, 1084, 2164, 2521
Eam1104I CTCTTC 2 cut(s) 1209, 1849
Eam1105I GACNNNNNGTC 1 cut(s) 1260
EarI CTCTTC 2 cut(s) 1209, 1849
EciI GGCGGA 1 cut(s) 2037
Eco105I TACGTA 1 cut(s) 2798
Eco130I CCWWGG 7 cut(s) 406, 430, 845, 1286, 1863, 2437, 2500
Eco24I GRGCYC 1 cut(s) 2500
Eco31I GGTCTC 2 cut(s) 420, 2762
Eco32I GATATC 1 cut(s) 390
Eco47I GGWCC 9 cut(s) 608, 1189, 1262, 1832, 1892, 1969, 2201, 2511, 2717
Eco57I CTGAAG 3 cut(s) 909, 978, 1782
Eco88I CYCGRG 1 cut(s) 2493
Eco91I GGTNACC 1 cut(s) 803
EcoNI CCTNNNNNAGG 1 cut(s) 1006
EcoO109I RGGNCCY 3 cut(s) 1172, 1832, 2201
EcoO65I GGTNACC 1 cut(s) 803
EcoRI GAATTC 1 cut(s) 481
EcoRII CCWGG 7 cut(s) 501, 1120, 1507, 1747, 2166, 2459, 2656
EcoRV GATATC 1 cut(s) 390
EcoT14I CCWWGG 7 cut(s) 406, 430, 845, 1286, 1863, 2437, 2500
EcoT22I ATGCAT 1 cut(s) 2317
EcoT38I GRGCYC 1 cut(s) 2500
ErhI CCWWGG 7 cut(s) 406, 430, 845, 1286, 1863, 2437, 2500
Esp3I CGTCTC 1 cut(s) 831
FaeI CATG 6 cut(s) 338, 917, 941, 1313, 1916, 2458
FalI AAGNNNNNCTT 2 cut(s) 1029, 1061
FaqI GGGAC 6 cut(s) 328, 424, 1125, 2582, 2762, 2804
FatI CATG 6 cut(s) 334, 913, 937, 1309, 1912, 2454
FauI CCCGC 1 cut(s) 2438
FbaI TGATCA 1 cut(s) 226
FblI GTMKAC 1 cut(s) 2800
Fnu4HI GCNGC 9 cut(s) 191, 194, 699, 702, 705, 1433, 1529, 1742, 1991
FokI GGATG 8 cut(s) 216, 236, 277, 889, 1456, 2131, 2476, 2747
FriOI GRGCYC 1 cut(s) 2500
Fsp4HI GCNGC 9 cut(s) 191, 194, 699, 702, 705, 1433, 1529, 1742, 1991
FspBI CTAG 8 cut(s) 30, 357, 846, 1356, 1410, 1734, 2031, 2763
GlaI GCGC 1 cut(s) 1329
GluI GCNGC 9 cut(s) 191, 194, 699, 702, 705, 1433, 1529, 1742, 1991
GsaI CCCAGC 5 cut(s) 1146, 1728, 1826, 1830, 2511
GsuI CTGGAG 2 cut(s) 1143, 2316
HaeIII GGCC 9 cut(s) 561, 1014, 1086, 1147, 1174, 1699, 2166, 2436, 2523
HapII CCGG 2 cut(s) 48, 1087
HgaI GACGC 3 cut(s) 978, 1622, 2811
HhaI GCGC 1 cut(s) 1330
Hin1I GRCGYC 1 cut(s) 2803
Hin1II CATG 6 cut(s) 338, 917, 941, 1313, 1916, 2458
Hin6I GCGC 1 cut(s) 1328
HinP1I GCGC 1 cut(s) 1328
HincII GTYRAC 2 cut(s) 2209, 2392
HindII GTYRAC 2 cut(s) 2209, 2392
HpaII CCGG 2 cut(s) 48, 1087
HphI GGTGA 3 cut(s) 446, 862, 2027
HpyCH4III ACNGT 5 cut(s) 70, 534, 636, 1116, 1973
HpyCH4IV ACGT 5 cut(s) 42, 528, 840, 2772, 2797
HpyF3I CTNAG 9 cut(s) 240, 274, 455, 762, 929, 1838, 1923, 2238, 2607
HpySE526I ACGT 5 cut(s) 42, 528, 840, 2772, 2797
Hsp92I GRCGYC 1 cut(s) 2803
Hsp92II CATG 6 cut(s) 338, 917, 941, 1313, 1916, 2458
HspAI GCGC 1 cut(s) 1328
KpnI GGTACC 2 cut(s) 1320, 1640
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 3 cut(s) 75, 226, 997
LguI GCTCTTC 1 cut(s) 1849
LmnI GCTCC 7 cut(s) 781, 976, 986, 1288, 1721, 2041, 2192
Lsp1109I GCAGC 7 cut(s) 202, 688, 710, 716, 1419, 1728, 2002
LweI GCATC 7 cut(s) 571, 867, 998, 1672, 2102, 2302, 2469
MabI ACCWGGT 2 cut(s) 1507, 1747
MaeI CTAG 8 cut(s) 30, 357, 846, 1356, 1410, 1734, 2031, 2763
MaeII ACGT 5 cut(s) 42, 528, 840, 2772, 2797
MaeIII GTNAC 5 cut(s) 297, 517, 803, 1500, 2059
MalI GATC 3 cut(s) 77, 228, 999
MboI GATC 3 cut(s) 75, 226, 997
MboII GAAGA 6 cut(s) 899, 984, 1196, 1622, 1836, 2570
MfeI CAATTG 2 cut(s) 1332, 2484
MflI RGATCY 1 cut(s) 997
MhlI GDGCHC 6 cut(s) 1069, 1285, 1426, 2038, 2197, 2500
MlsI TGGCCA 2 cut(s) 561, 2166
MluNI TGGCCA 2 cut(s) 561, 2166
MlyI GAGTC 5 cut(s) 89, 501, 737, 1532, 2356
MmeI TCCRAC 5 cut(s) 403, 1255, 1560, 2386, 2494
Mox20I TGGCCA 2 cut(s) 561, 2166
Mph1103I ATGCAT 1 cut(s) 2317
MroXI GAANNNNTTC 1 cut(s) 2628
MscI TGGCCA 2 cut(s) 561, 2166
MseI TTAA 5 cut(s) 84, 417, 1569, 2613, 2836
MslI CAYNNNNRTG 5 cut(s) 18, 576, 1062, 1308, 1395
Msp20I TGGCCA 2 cut(s) 561, 2166
MspA1I CMGCKG 3 cut(s) 193, 701, 1826
MspI CCGG 2 cut(s) 48, 1087
MspR9I CCNGG 8 cut(s) 49, 503, 1122, 1509, 1749, 2168, 2461, 2658
MunI CAATTG 2 cut(s) 1332, 2484
Mva1269I GAATGC 3 cut(s) 1144, 2368, 2656
MvaI CCWGG 7 cut(s) 503, 1122, 1509, 1749, 2168, 2461, 2658
NciI CCSGG 1 cut(s) 49
NdeII GATC 3 cut(s) 75, 226, 997
NlaIII CATG 6 cut(s) 338, 917, 941, 1313, 1916, 2458
NlaIV GGNNCC 8 cut(s) 499, 1013, 1318, 1638, 1833, 2512, 2544, 2645
NmeAIII GCCGAG 1 cut(s) 2499
NmuCI GTSAC 2 cut(s) 297, 1500
NsiI ATGCAT 1 cut(s) 2317
NspI RCATGY 3 cut(s) 338, 1313, 2458
OliI CACNNNNGTG 1 cut(s) 18
PaeR7I CTCGAG 1 cut(s) 2493
PaqCI CACCTGC 1 cut(s) 2267
PciI ACATGT 2 cut(s) 334, 2454
PciSI GCTCTTC 1 cut(s) 1849
PctI GAATGC 3 cut(s) 1144, 2368, 2656
PdmI GAANNNNTTC 1 cut(s) 2628
PfeI GAWTC 7 cut(s) 62, 1415, 1781, 1811, 1818, 2050, 2549
PfoI TCCNGGA 2 cut(s) 1120, 2459
PkrI GCNGC 9 cut(s) 192, 195, 700, 703, 706, 1434, 1530, 1743, 1992
PleI GAGTC 5 cut(s) 89, 500, 737, 1532, 2355
PpsI GAGTC 5 cut(s) 89, 500, 737, 1532, 2355
Ppu21I YACGTR 1 cut(s) 2798
PpuMI RGGWCCY 2 cut(s) 1832, 2201
PscI ACATGT 2 cut(s) 334, 2454
PshAI GACNNNNGTC 2 cut(s) 456, 2754
PsiI TTATAA 1 cut(s) 281
Psp1406I AACGTT 1 cut(s) 42
Psp5II RGGWCCY 2 cut(s) 1832, 2201
Psp6I CCWGG 7 cut(s) 501, 1120, 1507, 1747, 2166, 2459, 2656
PspEI GGTNACC 1 cut(s) 803
PspFI CCCAGC 5 cut(s) 1142, 1724, 1822, 1826, 2507
PspGI CCWGG 7 cut(s) 501, 1120, 1507, 1747, 2166, 2459, 2656
PspN4I GGNNCC 8 cut(s) 499, 1013, 1318, 1638, 1833, 2512, 2544, 2645
PspPPI RGGWCCY 2 cut(s) 1832, 2201
PsrI GAACNNNNNNTAC 2 cut(s) 1629, 1661
PstI CTGCAG 3 cut(s) 700, 706, 1345
PstNI CAGNNNCTG 3 cut(s) 196, 2156, 2245
PsuI RGATCY 1 cut(s) 997
PvuII CAGCTG 2 cut(s) 701, 1826
RsaI GTAC 6 cut(s) 531, 1236, 1318, 1638, 1705, 1930
RsaNI GTAC 6 cut(s) 530, 1235, 1317, 1637, 1704, 1929
RseI CAYNNNNRTG 5 cut(s) 18, 576, 1062, 1308, 1395
SapI GCTCTTC 1 cut(s) 1849
SaqAI TTAA 5 cut(s) 84, 417, 1569, 2613, 2836
SatI GCNGC 9 cut(s) 191, 194, 699, 702, 705, 1433, 1529, 1742, 1991
Sau3AI GATC 3 cut(s) 75, 226, 997
ScaI AGTACT 1 cut(s) 1705
SchI GAGTC 5 cut(s) 89, 501, 737, 1532, 2356
ScrFI CCNGG 8 cut(s) 49, 503, 1122, 1509, 1749, 2168, 2461, 2658
SduI GDGCHC 6 cut(s) 1069, 1285, 1426, 2038, 2197, 2500
SexAI ACCWGGT 2 cut(s) 1507, 1747
SfaNI GCATC 7 cut(s) 571, 867, 998, 1672, 2102, 2302, 2469
SfcI CTRYAG 4 cut(s) 696, 702, 1341, 2676
Sfr274I CTCGAG 1 cut(s) 2493
SinI GGWCC 9 cut(s) 608, 1189, 1262, 1832, 1892, 1969, 2201, 2511, 2717
SlaI CTCGAG 1 cut(s) 2493
SmiMI CAYNNNNRTG 5 cut(s) 18, 576, 1062, 1308, 1395
SmlI CTYRAG 3 cut(s) 1076, 2493, 2807
SmoI CTYRAG 3 cut(s) 1076, 2493, 2807
SnaBI TACGTA 1 cut(s) 2798
SsiI CCGC 5 cut(s) 193, 1529, 2022, 2445, 2715
SspI AATATT 1 cut(s) 82
SspMI CTAG 8 cut(s) 30, 357, 846, 1356, 1410, 1734, 2031, 2763
StyD4I CCNGG 8 cut(s) 47, 501, 1120, 1507, 1747, 2166, 2459, 2656
StyI CCWWGG 7 cut(s) 406, 430, 845, 1286, 1863, 2437, 2500
TaaI ACNGT 5 cut(s) 70, 534, 636, 1116, 1973
TaiI ACGT 5 cut(s) 45, 531, 843, 2775, 2800
TaqI TCGA 1 cut(s) 2494
TatI WGTACW 1 cut(s) 1703
TauI GCSGC 2 cut(s) 196, 1531
TfiI GAWTC 7 cut(s) 62, 1415, 1781, 1811, 1818, 2050, 2549
Tru1I TTAA 5 cut(s) 84, 417, 1569, 2613, 2836
Tru9I TTAA 5 cut(s) 84, 417, 1569, 2613, 2836
TscAI CASTG 3 cut(s) 25, 1443, 1909
TseFI GTSAC 2 cut(s) 297, 1500
TseI GCWGC 7 cut(s) 190, 698, 701, 704, 1432, 1741, 1990
Tsp45I GTSAC 2 cut(s) 297, 1500
TspDTI ATGAA 6 cut(s) 129, 297, 785, 1292, 1644, 2135
TspGWI ACGGA 1 cut(s) 588
TspRI CASTG 3 cut(s) 25, 1443, 1909
VneI GTGCAC 1 cut(s) 1422
VpaK11BI GGWCC 9 cut(s) 608, 1189, 1262, 1832, 1892, 1969, 2201, 2511, 2717
XagI CCTNNNNNAGG 1 cut(s) 1006
XapI RAATTY 7 cut(s) 481, 814, 853, 1017, 1028, 1585, 2686
XbaI TCTAGA 2 cut(s) 1409, 2762
XceI RCATGY 3 cut(s) 338, 1313, 2458
XcmI CCANNNNNNNNNTGG 1 cut(s) 2174
XhoI CTCGAG 1 cut(s) 2493
XmaJI CCTAGG 1 cut(s) 845
XmiI GTMKAC 1 cut(s) 2800
XmnI GAANNNNTTC 1 cut(s) 2628
XspI CTAG 8 cut(s) 30, 357, 846, 1356, 1410, 1734, 2031, 2763
ZrmI AGTACT 1 cut(s) 1705
Zsp2I ATGCAT 1 cut(s) 2317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.