RLG00000011029

Large proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005387
Physical Location & Seq
Forward (+)
6221381 .. 6229322
7942 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011029

Sequence Viewer

Length: 3039 bp
ATGGTTACCTTGATTTCACCATGTAAATCAACAACTGAACCAGTGGTGTTGGTGAATCGATTTGACACCAAATCTGAACATTCATCGCCCTCCACAGTTTGTATAATCTCGGCGCGCACAGAAAGGAAATATCGGAATCGCAGATACGGGTTTTCACTCATATTTAGCTTCACCGGAAACGCCGCCGTTGAATTGATTTTCCGACGTTCATTGCTCTTAACTAGCATGGCAAATCAGTACTCAAATGAAGGCTCTGGTCCCAGCAATGTTTCTGAGGAGACTTCTGATTCAACTGTTGAGATCAATATCAAGACTTTAGAGTCGCAGATATACAGTTTTCAAGTGGAAAAAAATATGCTGGTGTCATTGTTCAAAGAAAAAATAGCTGATCAAACAGGCGTCCCAGTTGGTCAGCAGCGGTTGATTTTCAGGGGAAAGGTGCTCAAGGATGATCATCCCCTTTCCGAGTATCATTTGGAAAATGGGCATACATTGCACTTAGTTGTGAGGCAGCCATCCCAGCCACAAACTTCATCTGGTACTAGTTCTGCTGAGCCTAATGCTAATGCTGGAAATGAGGGTAATGGGGCTCCTCGTGGTCGCATAGGGCAGATTTCACACAGTGTGGTTCTTGGGACCTTCAATGTTGGAGATCAAGGTGAAGGCATGGCCCCAGACCTTAGTCGGGTCATTGGCGCAGTTTTGAATTCTATTGGAATTGGCAACCAAGCTGCAACTAATGTTAATGGCAACATGCAGTCTACTATGCCAAATAATCCTGGTCAGTCTCCTGAAGGAAACGAAACAGAAGGGTTGCATGGTAATGCAAATAGTCAGACTCGAGGAGGAAATAATGCACAGTCTGGACTGACATTTTCTTCTCAGCCTTTCCAAACTTTGCCTCAATTTCTGCAAACTCCCCTTGCTGCTGGAGCATCTCCCTTTCCTTCACTAAATACGCCAATTCCGGACGCTTTGAACACTCTCTCTGAGTTTATGGACCGCATGGAGCGGGCATTATCACAAAATGGAGGCTACCAACAAAATATATCAGCAACAAACCCAGGAGACCTATCAAGGGTGAATTTACCTTCCAATGCTCAGGGGTTGCCCACGCCTGAAGCCTTGGGCATTGTTCTTCGCAATGCAGAACGGCTTCTCAGCGGTCATGCTGCTTCTGCACTATCTCACATTGCAGGGCGCTTGGAACAGGAGGGAGCATCTTCTGATCCCTCTGCAAGAGGCCAAATTCAGACAGAATCTATTCAAGTAGGGCTTGCAATGCAACATCTAGGTGCTCTTCTTCTTGAGCTGGGCCGGACAATTTGGACACTCCGCATGGGACAATCTCCTGGAGAAGCTGTTGTGAATGCTGGGCCAGCAGTTTATATTTCCCCATCAGGGCCTAATCCCATAATGGTGCAGCCTATTCCTTATCAAACAAGCTCCCCTTTTAGTGCTTCTGTACCCCTGTCGAATCCTATGAATTTTGGTCCTGTTGGCATTGGAAGTGCTCCAAGGAATGTAAACATTCATATACATGCTGGTACCTCACTCTCTGCACTTGGTGCTAGGGGAAGTAATGGGGAAGGGATGCAGGGAGAACATCGTAATGGACCTGGGTCAAGGGATTCAGGTGCAGCTCGAGTGCTGCCAGTGAGAAATATCATTGCTACCGCAATCCCATCATCCCAAACTGGTATTGCAACTTCCAGTGCATCACAGCCTGGTTCAGGTGTATCTGTACCACGGCCACGTTCAGACTCCTCTCTTTCGTCCATCGTTGCTGAACTTAATTCACAGATTAGAAACTTCGTTGGTAATGCTCAAGGAAATGATGCTGTTCAATCAGGTCAAGCGGTGCCCAATGTGCAGAATTCATCTGCTGGAGTTGGATCACATAATAATACAGGGAATGAGCAGCTCAGTAATTCAGATTTGAACGGAGGTCAGCAATCTAATGCGTCACTACCTGGTTCCACATCTGAAAGTGAAGTTCAGAAGGCTTCAGGAAGTGCACCTCCCTTGAGAGATGATTCAAGATTCCAAGCCAGAGATTCTCTTTCAAGTGGACAAAATCCAATGCCATCTGAAGATGACAAAGGAAATGCTTCACAAACTGCTGCAAAACAGGATAAGACCGAGGGCGCAAAAGCTGTTCCACTTGGATTAGGACTTGGAATGTTGGAACGTAAGAGACAAGGCAGGCAGCAAAAGACTCTTCAGAAGAATGGTGATGGTGGAACAACTAGCGCTTCCTCCAATCAGAATCAGCAAGTTACAAGTGGCCAACAACTTTTGCAGTCTCTTGCCACTCGTAGTACTGCTGGAAGTAGGTTGAGCACAAGTGATACTCCTGCAAGGCAAACAGTCCCCAATGTTGGGCAGGTTAGAGATGGCAGATCATTGGGAGGGCAAGGTCCTGGTGGCCAAGTTGATATGGGAAGTGTGATGTCTCAGGTCCTGCAGAGTCCTGCCTTAAATGGTCTGTTGTCAGGTGTTTCGGAGCAAACTGGGGTGGGTTCTCCTGATGCACTGCGGAATATGTTGCAGAACTTTACACAGAGTCCGCAGATGATGAATGCTGTCAACCAGATTACCGAACAGGTTGACACCCAAGATTTCGGAAACTTGTTTGCTGGGCTTGGAGGAGGCCAAGGTGGTGGAATTGATATGTCGAGGATGTTCCAACAGATGATGCCCATTGTATCTCGTGCCCTAGGGGCAGGGTCTTCTCCCGCTCAGCCACCCCCTGTCATGGGATCTCAGTCACTGTACAACGAGAGGAGTCTCAACAGAGATGATAATGAGATTGACCTCCAACAAGTGGTTCAAAGGATTGGGCAGCTGAATGCACCGGGAGATGTTTTTCAAGCTGTAGTTGAAAATTCAGTTCAGCTATCTGGCAGAGGAAGCAGTCCACAAGAACTTGTGGATGAGTTGTGCAGGGACGAGGGTCTCTCCAGTGAATACGATGAGATCTTACGGAGGGACATACGTCGAAGACTTGAGGGTGAATCGGGGCAGGAGAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001822 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010605 GO:0010941 GO:0012501 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031593 GO:0031647 GO:0032268 GO:0032269 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042981 GO:0043021 GO:0043022 GO:0043067 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045861 GO:0045995 GO:0048232 GO:0048285 GO:0048513 GO:0048519 GO:0048523 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070059 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1903046 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

1013

Amino Acids

106.29

Weight (kDa)

5.74

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 100 - 169 6.2e-08 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 101 - 171 1.1e-22 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 319
Acc36I ACCTGC 1 cut(s) 2377
Acc65I GGTACC 1 cut(s) 1547
AccB1I GGYRCC 2 cut(s) 1547, 1861
AccB7I CCANNNNNTGG 1 cut(s) 2827
AccBSI CCGCTC 2 cut(s) 1012, 2741
AccI GTMKAC 1 cut(s) 761
AccII CGCG 1 cut(s) 115
AccIII TCCGGA 1 cut(s) 967
AclWI GGATC 3 cut(s) 1223, 1903, 2770
AcoI YGGCCR 3 cut(s) 1751, 2287, 2428
AcsI RAATTY 6 cut(s) 706, 1084, 1248, 1486, 1876, 2887
AcuI CTGAAG 5 cut(s) 813, 1140, 1992, 2112, 2207
AcyI GRCGYC 1 cut(s) 399
AdeI CACNNNGTG 3 cut(s) 623, 625, 1568
AfaI GTAC 7 cut(s) 239, 541, 1467, 1549, 1746, 2323, 2777
AfeI AGCGCT 1 cut(s) 2254
AfiI CCNNNNNNNGG 9 cut(s) 685, 1078, 1401, 1733, 2381, 2382, 2758, 2759, 2827
AhlI ACTAGT 1 cut(s) 542
AjnI CCWGG 7 cut(s) 778, 1063, 1351, 1618, 1726, 1972, 2422
AjuI GAANNNNNNNTTGG 2 cut(s) 2814, 2846
Alw21I GWGCWC 5 cut(s) 444, 1300, 1516, 2020, 2345
Alw26I GTCTC 8 cut(s) 272, 792, 1062, 2191, 2310, 2460, 2795, 2963
Alw44I GTGCAC 1 cut(s) 2016
AlwI GGATC 3 cut(s) 1223, 1903, 2770
AlwNI CAGNNNCTG 2 cut(s) 2464, 2773
Ama87I CYCGRG 2 cut(s) 840, 1644
Aor13HI TCCGGA 1 cut(s) 967
Aor51HI AGCGCT 1 cut(s) 2254
AoxI GGCC 9 cut(s) 669, 1243, 1315, 1376, 1403, 1751, 2287, 2428, 2653
ApaLI GTGCAC 1 cut(s) 2016
ApoI RAATTY 6 cut(s) 706, 1084, 1248, 1486, 1876, 2887
Asp700I GAANNNNTTC 3 cut(s) 1155, 1263, 2110
Asp718I GGTACC 1 cut(s) 1547
AspA2I CCTAGG 1 cut(s) 2719
AspLEI GCGC 6 cut(s) 115, 117, 698, 1203, 2150, 2255
AsuC2I CCSGG 1 cut(s) 2859
AsuHPI GGTGA 7 cut(s) 9, 64, 163, 671, 1093, 2246, 3026
AvaI CYCGRG 2 cut(s) 840, 1644
AvaII GGWCC 7 cut(s) 257, 636, 1000, 1493, 1616, 2420, 2461
AvrII CCTAGG 1 cut(s) 2719
BaeGI GKGCMC 3 cut(s) 1866, 2020, 2719
BalI TGGCCA 2 cut(s) 2289, 2430
BanI GGYRCC 2 cut(s) 1547, 1861
BanII GRGCYC 1 cut(s) 592
BarI GAAGNNNNNNTAC 2 cut(s) 1693, 1725
BauI CACGAG 2 cut(s) 594, 2712
BbsI GAAGAC 2 cut(s) 2724, 3010
Bbv12I GWGCWC 5 cut(s) 444, 1300, 1516, 2020, 2345
BccI CCATC 7 cut(s) 523, 1405, 1693, 1787, 2095, 2231, 2390
BceAI ACGGC 3 cut(s) 170, 1169, 1766
BciT130I CCWGG 7 cut(s) 780, 1065, 1353, 1620, 1728, 1974, 2424
BclI TGATCA 2 cut(s) 388, 451
BcnI CCSGG 1 cut(s) 2859
BcoDI GTCTC 8 cut(s) 272, 792, 1062, 2191, 2310, 2460, 2795, 2963
BcuI ACTAGT 1 cut(s) 542
BfaI CTAG 6 cut(s) 222, 543, 1292, 1572, 2250, 2720
BfmI CTRYAG 2 cut(s) 2465, 2877
BfoI RGCGCY 2 cut(s) 1204, 2256
BfuAI ACCTGC 1 cut(s) 2377
BglI GCCNNNNNGGC 1 cut(s) 2723
BglII AGATCT 1 cut(s) 2979
BlnI CCTAGG 1 cut(s) 2719
BlpI GCTNAGC 2 cut(s) 552, 2742
BmcAI AGTACT 2 cut(s) 239, 2323
Bme1390I CCNGG 8 cut(s) 780, 1065, 1353, 1620, 1728, 1974, 2424, 2859
Bme18I GGWCC 7 cut(s) 257, 636, 1000, 1493, 1616, 2420, 2461
BmeT110I CYCGRG 2 cut(s) 840, 1644
BmiI GGNNCC 7 cut(s) 259, 591, 637, 672, 1549, 1863, 1978
BmrFI CCNGG 8 cut(s) 780, 1065, 1353, 1620, 1728, 1974, 2424, 2859
BmrI ACTGGG 2 cut(s) 398, 2523
BmsI GCATC 7 cut(s) 944, 1229, 1584, 1727, 1828, 2521, 2688
BmuI ACTGGG 2 cut(s) 398, 2523
BoxI GACNNNNGTC 4 cut(s) 681, 1621, 2955, 2997
BpiI GAAGAC 2 cut(s) 2724, 3010
BplI GAGNNNNNCTC 2 cut(s) 2945, 2977
BpmI CTGGAG 4 cut(s) 951, 1374, 1908, 2947
Bpu10I CCTNAGC 1 cut(s) 1101
Bpu1102I GCTNAGC 2 cut(s) 552, 2742
BpuEI CTTGAG 5 cut(s) 428, 1328, 1812, 2047, 3029
BpuMI CCSGG 1 cut(s) 2859
Bsa29I ATCGAT 1 cut(s) 58
BsaBI GATNNNNATC 2 cut(s) 305, 453
BsaHI GRCGYC 1 cut(s) 399
BsaI GGTCTC 2 cut(s) 1062, 2963
BsaJI CCNNGG 8 cut(s) 1063, 1125, 1517, 1619, 1748, 2142, 2656, 2719
BsaWI WCCGGW 2 cut(s) 173, 967
BsaXI ACNNNNNCTCC 2 cut(s) 1347, 1377
Bsc4I CCNNNNNNNGG 9 cut(s) 685, 1078, 1401, 1733, 2381, 2382, 2758, 2759, 2827
Bse1I ACTGG 7 cut(s) 41, 404, 1655, 1702, 1713, 2518, 2964
Bse3DI GCAATG 7 cut(s) 209, 271, 491, 1150, 1191, 1287, 1668
Bse8I GATNNNNATC 2 cut(s) 305, 453
BseAI TCCGGA 1 cut(s) 967
BseBI CCWGG 7 cut(s) 780, 1065, 1353, 1620, 1728, 1974, 2424
BseCI ATCGAT 1 cut(s) 58
BseDI CCNNGG 8 cut(s) 1063, 1125, 1517, 1619, 1748, 2142, 2656, 2719
BseGI GGATG 7 cut(s) 454, 454, 515, 1599, 1688, 2688, 2941
BseJI GATNNNNATC 2 cut(s) 305, 453
BseLI CCNNNNNNNGG 9 cut(s) 685, 1078, 1401, 1733, 2381, 2382, 2758, 2759, 2827
BseMI GCAATG 7 cut(s) 209, 271, 491, 1150, 1191, 1287, 1668
BseNI ACTGG 7 cut(s) 41, 404, 1655, 1702, 1713, 2518, 2964
BsePI GCGCGC 1 cut(s) 113
BseRI GAGGAG 6 cut(s) 290, 582, 858, 1756, 2664, 2800
BseSI GKGCMC 3 cut(s) 1866, 2020, 2719
BseYI CCCAGC 5 cut(s) 260, 519, 1312, 1373, 2639
BsgI GTGCAG 6 cut(s) 1164, 1442, 1545, 1659, 1892, 2965
Bsh1236I CGCG 1 cut(s) 115
BshFI GGCC 9 cut(s) 671, 1245, 1317, 1378, 1405, 1753, 2289, 2430, 2655
BshNI GGYRCC 2 cut(s) 1547, 1861
BshVI ATCGAT 1 cut(s) 58
BsiHKAI GWGCWC 5 cut(s) 444, 1300, 1516, 2020, 2345
BsiHKCI CYCGRG 2 cut(s) 840, 1644
BsiSI CCGG 4 cut(s) 174, 968, 1318, 2858
BslFI GGGAC 7 cut(s) 243, 386, 649, 1356, 2357, 2963, 3005
BslI CCNNNNNNNGG 9 cut(s) 685, 1078, 1401, 1733, 2381, 2382, 2758, 2759, 2827
BsmAI GTCTC 8 cut(s) 272, 792, 1062, 2191, 2310, 2460, 2795, 2963
BsmFI GGGAC 7 cut(s) 243, 386, 649, 1356, 2357, 2963, 3005
BsmI GAATGC 3 cut(s) 1375, 2587, 2857
BsnI GGCC 9 cut(s) 671, 1245, 1317, 1378, 1405, 1753, 2289, 2430, 2655
Bso31I GGTCTC 2 cut(s) 1062, 2963
BsoBI CYCGRG 2 cut(s) 840, 1644
Bsp1286I GDGCHC 8 cut(s) 444, 592, 1300, 1516, 1866, 2020, 2345, 2719
Bsp13I TCCGGA 1 cut(s) 967
Bsp1407I TGTACA 1 cut(s) 2775
Bsp143I GATC 9 cut(s) 300, 388, 451, 652, 1228, 1895, 2402, 2762, 2979
Bsp1720I GCTNAGC 2 cut(s) 552, 2742
BspANI GGCC 9 cut(s) 671, 1245, 1317, 1378, 1405, 1753, 2289, 2430, 2655
BspDI ATCGAT 1 cut(s) 58
BspEI TCCGGA 1 cut(s) 967
BspFNI CGCG 1 cut(s) 115
BspLI GGNNCC 7 cut(s) 259, 591, 637, 672, 1549, 1863, 1978
BspMAI CTGCAG 1 cut(s) 2469
BspMI ACCTGC 1 cut(s) 2377
BspPI GGATC 3 cut(s) 1223, 1903, 2770
BspQI GCTCTTC 1 cut(s) 1305
BspT107I GGYRCC 2 cut(s) 1547, 1861
BspTNI GGTCTC 2 cut(s) 1062, 2963
BsrBI CCGCTC 2 cut(s) 1012, 2741
BsrDI GCAATG 7 cut(s) 209, 271, 491, 1150, 1191, 1287, 1668
BsrGI TGTACA 1 cut(s) 2775
BsrI ACTGG 7 cut(s) 41, 404, 1655, 1702, 1713, 2518, 2964
BssECI CCNNGG 8 cut(s) 1063, 1125, 1517, 1619, 1748, 2142, 2656, 2719
BssHII GCGCGC 1 cut(s) 113
BssMI GATC 9 cut(s) 300, 388, 451, 652, 1228, 1895, 2402, 2762, 2979
BssNI GRCGYC 1 cut(s) 399
BssSI CACGAG 2 cut(s) 594, 2712
BssT1I CCWWGG 4 cut(s) 1125, 1517, 2656, 2719
Bst2BI CACGAG 2 cut(s) 594, 2712
Bst2UI CCWGG 7 cut(s) 780, 1065, 1353, 1620, 1728, 1974, 2424
Bst4CI ACNGT 7 cut(s) 97, 295, 335, 623, 861, 2371, 2775
Bst6I CTCTTC 2 cut(s) 1305, 2226
BstACI GRCGYC 1 cut(s) 399
BstAPI GCANNNNNTGC 2 cut(s) 493, 1568
BstAUI TGTACA 1 cut(s) 2775
BstC8I GCNNGC 5 cut(s) 115, 1014, 1278, 1380, 2207
BstDSI CCRYGG 1 cut(s) 1748
BstEII GGTNACC 1 cut(s) 4
BstENI CCTNNNNNAGG 1 cut(s) 1731
BstF5I GGATG 7 cut(s) 454, 454, 515, 1599, 1688, 2688, 2941
BstFNI CGCG 1 cut(s) 115
BstH2I RGCGCY 2 cut(s) 1204, 2256
BstHHI GCGC 6 cut(s) 115, 117, 698, 1203, 2150, 2255
BstKTI GATC 9 cut(s) 303, 391, 454, 655, 1231, 1898, 2405, 2765, 2982
BstMAI GTCTC 8 cut(s) 272, 792, 1062, 2191, 2310, 2460, 2795, 2963
BstMBI GATC 9 cut(s) 300, 388, 451, 652, 1228, 1895, 2402, 2762, 2979
BstNI CCWGG 7 cut(s) 780, 1065, 1353, 1620, 1728, 1974, 2424
BstNSI RCATGY 2 cut(s) 757, 1544
BstPAI GACNNNNGTC 4 cut(s) 681, 1621, 2955, 2997
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 8 cut(s) 778, 1063, 1351, 1618, 1726, 1972, 2422, 2857
BstSFI CTRYAG 2 cut(s) 2465, 2877
BstSLI GKGCMC 3 cut(s) 1866, 2020, 2719
BstUI CGCG 1 cut(s) 115
BstV2I GAAGAC 2 cut(s) 2724, 3010
BstX2I RGATCY 2 cut(s) 2762, 2979
BstXI CCANNNNNNTGG 1 cut(s) 2663
BstYI RGATCY 2 cut(s) 2762, 2979
Bsu15I ATCGAT 1 cut(s) 58
BsuRI GGCC 9 cut(s) 671, 1245, 1317, 1378, 1405, 1753, 2289, 2430, 2655
BsuTUI ATCGAT 1 cut(s) 58
BtgI CCRYGG 1 cut(s) 1748
BtgZI GCGATG 1 cut(s) 69
BtsCI GGATG 7 cut(s) 454, 454, 515, 1599, 1688, 2688, 2941
BtsI GCAGTG 1 cut(s) 2534
BtsIMutI CAGTG 7 cut(s) 48, 628, 1662, 1720, 2534, 2771, 2971
BveI ACCTGC 1 cut(s) 2377
Cac8I GCNNGC 5 cut(s) 115, 1014, 1278, 1380, 2207
CaiI CAGNNNCTG 2 cut(s) 2464, 2773
CfoI GCGC 6 cut(s) 115, 117, 698, 1203, 2150, 2255
ClaI ATCGAT 1 cut(s) 58
CseI GACGC 3 cut(s) 388, 980, 1953
CsiI ACCWGGT 1 cut(s) 1972
Csp6I GTAC 7 cut(s) 238, 540, 1466, 1548, 1745, 2322, 2776
CviQI GTAC 7 cut(s) 238, 540, 1466, 1548, 1745, 2322, 2776
DpnI GATC 9 cut(s) 302, 390, 453, 654, 1230, 1897, 2404, 2764, 2981
DpnII GATC 9 cut(s) 300, 388, 451, 652, 1228, 1895, 2402, 2762, 2979
DraIII CACNNNGTG 3 cut(s) 623, 625, 1568
DrdI GACNNNNNNGTC 1 cut(s) 319
DseDI GACNNNNNNGTC 1 cut(s) 319
EaeI YGGCCR 3 cut(s) 1751, 2287, 2428
Eam1104I CTCTTC 2 cut(s) 1305, 2226
EarI CTCTTC 2 cut(s) 1305, 2226
Eco130I CCWWGG 4 cut(s) 1125, 1517, 2656, 2719
Eco24I GRGCYC 1 cut(s) 592
Eco31I GGTCTC 2 cut(s) 1062, 2963
Eco47I GGWCC 7 cut(s) 257, 636, 1000, 1493, 1616, 2420, 2461
Eco47III AGCGCT 1 cut(s) 2254
Eco57I CTGAAG 5 cut(s) 813, 1140, 1992, 2112, 2207
Eco88I CYCGRG 2 cut(s) 840, 1644
Eco91I GGTNACC 1 cut(s) 4
EcoNI CCTNNNNNAGG 1 cut(s) 1731
EcoO109I RGGNCCY 4 cut(s) 636, 1403, 2420, 2461
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 2 cut(s) 706, 1876
EcoRII CCWGG 7 cut(s) 778, 1063, 1351, 1618, 1726, 1972, 2422
EcoT14I CCWWGG 4 cut(s) 1125, 1517, 2656, 2719
EcoT38I GRGCYC 1 cut(s) 592
ErhI CCWWGG 4 cut(s) 1125, 1517, 2656, 2719
FalI AAGNNNNNCTT 4 cut(s) 1260, 1292, 1435, 1467
FaqI GGGAC 7 cut(s) 243, 386, 649, 1356, 2357, 2963, 3005
FauI CCCGC 2 cut(s) 1005, 2746
FbaI TGATCA 2 cut(s) 388, 451
FblI GTMKAC 1 cut(s) 761
FokI GGATG 7 cut(s) 441, 461, 502, 1606, 1675, 2695, 2948
FriOI GRGCYC 1 cut(s) 592
FspBI CTAG 6 cut(s) 222, 543, 1292, 1572, 2250, 2720
GlaI GCGC 6 cut(s) 114, 116, 697, 1202, 2149, 2254
GsaI CCCAGC 5 cut(s) 264, 523, 1316, 1377, 2643
GsuI CTGGAG 4 cut(s) 951, 1374, 1908, 2947
HaeII RGCGCY 2 cut(s) 1204, 2256
HaeIII GGCC 9 cut(s) 671, 1245, 1317, 1378, 1405, 1753, 2289, 2430, 2655
HapII CCGG 4 cut(s) 174, 968, 1318, 2858
HgaI GACGC 3 cut(s) 388, 980, 1953
HhaI GCGC 6 cut(s) 115, 117, 698, 1203, 2150, 2255
Hin1I GRCGYC 1 cut(s) 399
Hin6I GCGC 6 cut(s) 113, 115, 696, 1201, 2148, 2253
HinP1I GCGC 6 cut(s) 113, 115, 696, 1201, 2148, 2253
HincII GTYRAC 2 cut(s) 2590, 2611
HindII GTYRAC 2 cut(s) 2590, 2611
HpaII CCGG 4 cut(s) 174, 968, 1318, 2858
HphI GGTGA 7 cut(s) 9, 64, 163, 671, 1093, 2246, 3026
Hpy166II GTNNAC 7 cut(s) 762, 1528, 2018, 2072, 2590, 2611, 2921
Hpy188III TCNNGA 8 cut(s) 310, 791, 864, 968, 1307, 2010, 2040, 2528
Hpy8I GTNNAC 7 cut(s) 762, 1528, 2018, 2072, 2590, 2611, 2921
Hpy99I CGWCG 2 cut(s) 207, 3003
HpyAV CCTTC 9 cut(s) 242, 649, 656, 788, 803, 957, 1101, 1583, 1996
HpyCH4III ACNGT 7 cut(s) 97, 295, 335, 623, 861, 2371, 2775
HpyCH4IV ACGT 4 cut(s) 205, 1756, 2191, 2998
HpySE526I ACGT 4 cut(s) 205, 1756, 2191, 2998
Hsp92I GRCGYC 1 cut(s) 399
HspAI GCGC 6 cut(s) 113, 115, 696, 1201, 2148, 2253
Kpn2I TCCGGA 1 cut(s) 967
KpnI GGTACC 1 cut(s) 1551
Ksp22I TGATCA 2 cut(s) 388, 451
Kzo9I GATC 9 cut(s) 300, 388, 451, 652, 1228, 1895, 2402, 2762, 2979
LguI GCTCTTC 1 cut(s) 1305
LmnI GCTCC 7 cut(s) 595, 932, 1009, 1217, 1451, 1519, 2506
LweI GCATC 7 cut(s) 944, 1229, 1584, 1727, 1828, 2521, 2688
MabI ACCWGGT 1 cut(s) 1972
MaeI CTAG 6 cut(s) 222, 543, 1292, 1572, 2250, 2720
MaeII ACGT 4 cut(s) 205, 1756, 2191, 2998
MaeIII GTNAC 4 cut(s) 4, 1965, 2278, 2769
MalI GATC 9 cut(s) 302, 390, 453, 654, 1230, 1897, 2404, 2764, 2981
MbiI CCGCTC 2 cut(s) 1012, 2741
MboI GATC 9 cut(s) 300, 388, 451, 652, 1228, 1895, 2402, 2762, 2979
MflI RGATCY 2 cut(s) 2762, 2979
MhlI GDGCHC 8 cut(s) 444, 592, 1300, 1516, 1866, 2020, 2345, 2719
MlsI TGGCCA 2 cut(s) 2289, 2430
MluNI TGGCCA 2 cut(s) 2289, 2430
MlyI GAGTC 7 cut(s) 329, 832, 1757, 2212, 2479, 2575, 2797
MmeI TCCRAC 6 cut(s) 226, 628, 1873, 2166, 2713, 2845
Mox20I TGGCCA 2 cut(s) 2289, 2430
MroI TCCGGA 1 cut(s) 967
MroXI GAANNNNTTC 3 cut(s) 1155, 1263, 2110
MscI TGGCCA 2 cut(s) 2289, 2430
MseI TTAA 4 cut(s) 218, 744, 1794, 2480
MslI CAYNNNNRTG 5 cut(s) 822, 1293, 1418, 1539, 1611
Msp20I TGGCCA 2 cut(s) 2289, 2430
MspA1I CMGCKG 3 cut(s) 418, 1164, 2848
MspI CCGG 4 cut(s) 174, 968, 1318, 2858
MspR9I CCNGG 8 cut(s) 780, 1065, 1353, 1620, 1728, 1974, 2424, 2859
Mva1269I GAATGC 3 cut(s) 1375, 2587, 2857
MvaI CCWGG 7 cut(s) 780, 1065, 1353, 1620, 1728, 1974, 2424
MvnI CGCG 1 cut(s) 115
NciI CCSGG 1 cut(s) 2859
NdeII GATC 9 cut(s) 300, 388, 451, 652, 1228, 1895, 2402, 2762, 2979
NlaIV GGNNCC 7 cut(s) 259, 591, 637, 672, 1549, 1863, 1978
NmeAIII GCCGAG 1 cut(s) 89
NmuCI GTSAC 2 cut(s) 1965, 2769
NspI RCATGY 2 cut(s) 757, 1544
PaeR7I CTCGAG 2 cut(s) 840, 1644
PauI GCGCGC 1 cut(s) 113
PciSI GCTCTTC 1 cut(s) 1305
PctI GAATGC 3 cut(s) 1375, 2587, 2857
PdmI GAANNNNTTC 3 cut(s) 1155, 1263, 2110
PflMI CCANNNNNTGG 1 cut(s) 2827
PfoI TCCNGGA 1 cut(s) 1351
PleI GAGTC 7 cut(s) 328, 832, 1757, 2212, 2478, 2574, 2796
PpsI GAGTC 7 cut(s) 328, 832, 1757, 2212, 2478, 2574, 2796
PpuMI RGGWCCY 3 cut(s) 636, 2420, 2461
PshAI GACNNNNGTC 4 cut(s) 681, 1621, 2955, 2997
Psp5II RGGWCCY 3 cut(s) 636, 2420, 2461
Psp6I CCWGG 7 cut(s) 778, 1063, 1351, 1618, 1726, 1972, 2422
PspEI GGTNACC 1 cut(s) 4
PspFI CCCAGC 5 cut(s) 260, 519, 1312, 1373, 2639
PspGI CCWGG 7 cut(s) 778, 1063, 1351, 1618, 1726, 1972, 2422
PspN4I GGNNCC 7 cut(s) 259, 591, 637, 672, 1549, 1863, 1978
PspPPI RGGWCCY 3 cut(s) 636, 2420, 2461
PspXI VCTCGAGB 2 cut(s) 840, 1644
PstI CTGCAG 1 cut(s) 2469
PstNI CAGNNNCTG 2 cut(s) 2464, 2773
PsuI RGATCY 2 cut(s) 2762, 2979
PteI GCGCGC 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 2848
RsaI GTAC 7 cut(s) 239, 541, 1467, 1549, 1746, 2323, 2777
RsaNI GTAC 7 cut(s) 238, 540, 1466, 1548, 1745, 2322, 2776
RseI CAYNNNNRTG 5 cut(s) 822, 1293, 1418, 1539, 1611
SapI GCTCTTC 1 cut(s) 1305
SaqAI TTAA 4 cut(s) 218, 744, 1794, 2480
Sau3AI GATC 9 cut(s) 300, 388, 451, 652, 1228, 1895, 2402, 2762, 2979
ScaI AGTACT 2 cut(s) 239, 2323
SchI GAGTC 7 cut(s) 329, 832, 1757, 2212, 2479, 2575, 2797
ScrFI CCNGG 8 cut(s) 780, 1065, 1353, 1620, 1728, 1974, 2424, 2859
SduI GDGCHC 8 cut(s) 444, 592, 1300, 1516, 1866, 2020, 2345, 2719
SexAI ACCWGGT 1 cut(s) 1972
SfaNI GCATC 7 cut(s) 944, 1229, 1584, 1727, 1828, 2521, 2688
SfcI CTRYAG 2 cut(s) 2465, 2877
Sfr274I CTCGAG 2 cut(s) 840, 1644
SinI GGWCC 7 cut(s) 257, 636, 1000, 1493, 1616, 2420, 2461
SlaI CTCGAG 2 cut(s) 840, 1644
SmiMI CAYNNNNRTG 5 cut(s) 822, 1293, 1418, 1539, 1611
SmlI CTYRAG 7 cut(s) 443, 840, 1307, 1644, 1827, 2026, 3008
SmoI CTYRAG 7 cut(s) 443, 840, 1307, 1644, 1827, 2026, 3008
SpeI ACTAGT 1 cut(s) 542
SspMI CTAG 6 cut(s) 222, 543, 1292, 1572, 2250, 2720
StyD4I CCNGG 8 cut(s) 778, 1063, 1351, 1618, 1726, 1972, 2422, 2857
StyI CCWWGG 4 cut(s) 1125, 1517, 2656, 2719
TaaI ACNGT 7 cut(s) 97, 295, 335, 623, 861, 2371, 2775
TaiI ACGT 4 cut(s) 208, 1759, 2194, 3001
TaqI TCGA 6 cut(s) 58, 841, 1475, 1645, 2678, 3001
TaqII GACCGA 1 cut(s) 2156
TatI WGTACW 3 cut(s) 237, 2321, 2775
TauI GCSGC 1 cut(s) 185
Tru1I TTAA 4 cut(s) 218, 744, 1794, 2480
Tru9I TTAA 4 cut(s) 218, 744, 1794, 2480
TscAI CASTG 7 cut(s) 48, 628, 1662, 1720, 2541, 2778, 2971
TseFI GTSAC 2 cut(s) 1965, 2769
Tsp45I GTSAC 2 cut(s) 1965, 2769
TspDTI ATGAA 8 cut(s) 72, 198, 261, 522, 1499, 1523, 1869, 2594
TspGWI ACGGA 2 cut(s) 1959, 3001
TspRI CASTG 7 cut(s) 48, 628, 1662, 1720, 2541, 2778, 2971
Van91I CCANNNNNTGG 1 cut(s) 2827
VneI GTGCAC 1 cut(s) 2016
VpaK11BI GGWCC 7 cut(s) 257, 636, 1000, 1493, 1616, 2420, 2461
XagI CCTNNNNNAGG 1 cut(s) 1731
XapI RAATTY 6 cut(s) 706, 1084, 1248, 1486, 1876, 2887
XceI RCATGY 2 cut(s) 757, 1544
XhoI CTCGAG 2 cut(s) 840, 1644
XmaJI CCTAGG 1 cut(s) 2719
XmiI GTMKAC 1 cut(s) 761
XmnI GAANNNNTTC 3 cut(s) 1155, 1263, 2110
XspI CTAG 6 cut(s) 222, 543, 1292, 1572, 2250, 2720
ZrmI AGTACT 2 cut(s) 239, 2323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.