MD15G1004200.v1.1

Large proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005387
Physical Location & Seq
Forward (+)
250771 .. 257458
6688 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1004200.v1.1.491

Sequence Viewer

Length: 2757 bp
ATGGAAGATCAGCACTCCAACGAAGGCGCCAGTTCTGGCAATGTTGTTGGGGCCACTTCTGATTCAAATCTTGAGATCAATATTAAGACTTTAGACTCACAGATGTACAGTTTTCAAGTTGATAAAAATATGCGAGTTTCACTGTTCAAGGAAAAAATAGCTGATCAAACAGGTGTACCAGTCAGTCAGCAGCGGCTGATTTTCAGGGGAAGGGTGTTAAAAGATGACCATCCTCTTTCTGAGTATCATTTGGAAAATGGGCACACATTGCACTTAGTTATTAGGCAGCCATCCCAGCCACAACCTTCATCGGGTACAAGTTCTGGCGAGGCACATGTGAATAGTGGAAATGATGCTAGTGGTGTTCCTCGTGGTCGCATTGGCCAGATATCTCACAGTGTAGTTCTTGGGACATTTAATGTTGGAGATCAAGGTGAAGGCGCTGTTCCTGATCTTAGTCGGCTTATTGGCACGGTTCTGAATTCTATTGGAATTGGCACCCAGGCTACAACTAATGGCACAGGCAACCCACAGTTCACTACTTCGTCAAACACTCCTGGTCAGCCTCGTAATGGAAATGAAACAGAAGGGTCTCATAATGTCAACGGAGGAGGACATCAAGCACAGTCTGGACAGGCATTTCCTACCCAACCATTCCAAACTTTTCCTCAAGCTGTGCAAACTCCTCTTGCAGCTGCAGCATTTCCTATGCCTTCATTAAATATGCCAATTCCACACTCTTTGGATACACTCTCCGAGTTCATGAAGAGAATGGAGCAGGGATTATCGCAAAATGGTTATCAGCCAAATACATCTGCAACCAATACAGGAGACCCACCTAGGGTGAACTTACCTTCCACTGCACAGGGGATGCCCACACCTGAGGCATTGGGGATTGTTCTTCGTCATGTAGAGCGACTACTCAGCAATCATGCTGTTTCTGCGCTATCTCACATTGCAGGACGATTGGAACAAGATGGAGCATCTTCTGATCCCTCTGTGAGAGGTCAAATTCAGACAGAATCTATACAATTAGGACTTGCAATGCAACATTTAGGTTCTCTTCTTCTTGAGCTTGGCCGAACAATTTGGACCCTTCGTATGGGGCAGTCTCCTGGAGAAGCTGTTGTGAATGCTGGGCCAGCAGTTTACATATCCCCATCAGGACCTAATCCCATAATGGTCCAGCCTTTCCCTCTTCAAACAAGCTCACTCATTGGTGGTTCTGTACCTCAATCAAACCCTGTGAGTTTTGGTCCTGTTGGGATTGGCAGTGCTCCAAGGAATGTAAACATCCATATACATGCTGTTGGTGCTAGGGGTAGTAATGGGGAGGGTATCCCATCTGGTTCTAGGGATTCAGGTGGCCGGGTGCTGCCAGTGAGAAATGTAGTCGGTGCCACTATTCCATCCTCCCAAATTGGTGTTTCAGTATCCAATGCGTCACAACCCGGTTCTGGTGTTTCTGTTTCACAGCAGCCTTCTGGCTCCTCACTTTCCTCCATTGTTGCTGAACTTAACTCACATTTTAGAAGTTTTGTTGGCGATACACAAGCAGAAGACACAGTTCAATCAGGTCAGGATGTGTCCACTGTTCAGAATCCATCTGTTGAACCGCATAATTATGCAGGAAGTGAACCTCCAAGTACTGGCTATGTCGATATAGCTGGTGTGTCACTACCTGGTTGCACATCTGAAAGTGAAGGTCAGAAGGATTCAGGAAGTGTTGGTACCTTGAAAAACAATTCAATATTCCCAGTTGGAGGTTCTCTAAGCTCTTCAAGTGGCCAAAACACCGTGGTGAGAGAGGATGAGAGGGGAAATGCTCCACAATCCAGTGAGAAGCAGGCTGAAGGTGCGAAATCTGCTCCACTTGGTTTAGGACTCGGAGTTTTGGAGCGTAAGAGACAAGCCAGGCAGCCAAAACCGCTGACCAAGAATGAAGATGGTGGAATAACTAGTTTTCCCATCAATCAGAATCAGCAAGTAATAGGTGGCCAGCAAGTTTTGCAGTCCCTTGCATCTTGTAGTTCTGCTGTAAGTAGGATGAATTCAAGTGATGTGCCGGCAAGACAAACAGTGCCTGCTGTTGAGCAGGTCAGAGATGGTAGAACATTGGGAGGGCAAGGTCCTGTTGGCCAAGTTGATATGGGAAGCATGATGTCTCAGGTTCTGCAGAGTCCAGCCCTGAATGGTCTGTTGGCAGGTGTTTCAGACCAAACGGGAGTGGGTTCTCCCGATATATTGAGGAATATGTTACAGAACTTTACACAGAATCCACAGATGAGGAATGCTGTCAATCAGATTGCTGAACAGGTTGACAGCCAAGATTTGGGAAACTTGTTTGGAGGAGGCCAAGGTGGTGGGATTGATATGTCCAGGATGTTCCAACAAATGATGCCCATTGTAACTCGCGCCCTTGGGGCTGGATCAAATCCCGTCCGGCCATCCTCTGCTTTGGTTCCTGAATCTCGACCACCCCACAATGAGCGAGGTCTCAGTAGAGATGATAATATCCCTAAGTCTGAGATTAATCTCCAAGAAGTGGTTCAGAGGATTGAGAACCTGAATGCACCGGGAGATGTTTTTCATGCTCTAGTTGAGAATTCAGTTCAGCTGTCTGGCAGAGGAAGTGGTCCTCAAGAACTTGTGGATGAGTTGTGCAGGGACGAGCATCTCTCCAGTGAATATGTTGAAATATTACGCAGGGACGTACGGCGACGGCTTGAGGGTGATTCAGGGAAGGACAAGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001822 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010605 GO:0010941 GO:0012501 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031593 GO:0031647 GO:0032268 GO:0032269 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042981 GO:0043021 GO:0043022 GO:0043067 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045861 GO:0045995 GO:0048232 GO:0048285 GO:0048513 GO:0048519 GO:0048523 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070059 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1903046 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

919

Amino Acids

96.29

Weight (kDa)

5.59

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 25 - 94 1.3e-09 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 26 - 96 4.8e-24 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2195
Acc36I ACCTGC 2 cut(s) 2086, 2195
Acc65I GGTACC 1 cut(s) 1730
AccB1I GGYRCC 4 cut(s) 26, 497, 1397, 1730
AccB7I CCANNNNNTGG 3 cut(s) 1649, 2332, 2545
AccII CGCG 1 cut(s) 2415
AciI CCGC 3 cut(s) 193, 1616, 1928
AclWI GGATC 2 cut(s) 986, 2437
AcoI YGGCCR 7 cut(s) 382, 1078, 1366, 1786, 1996, 2137, 2444
AcsI RAATTY 4 cut(s) 481, 1011, 2050, 2605
AcuI CTGAAG 1 cut(s) 1872
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 7 cut(s) 107, 177, 316, 1230, 1648, 1732, 2715
AfiI CCNNNNNNNGG 9 cut(s) 311, 572, 841, 1102, 1457, 1649, 1763, 2332, 2545
AflIII ACRYGT 1 cut(s) 334
AhlI ACTAGT 1 cut(s) 1958
AjnI CCWGG 6 cut(s) 501, 556, 1114, 1681, 1913, 2378
AjuI GAANNNNNNNTTGG 4 cut(s) 2183, 2215, 2532, 2564
AleI CACNNNNGTG 1 cut(s) 1799
AloI GAACNNNNNNTCC 2 cut(s) 839, 871
AluBI AGCT 9 cut(s) 161, 674, 695, 1075, 1124, 1209, 1667, 1776, 2617
AluI AGCT 9 cut(s) 161, 674, 695, 1075, 1124, 1209, 1667, 1776, 2617
Alw21I GWGCWC 1 cut(s) 1279
Alw26I GTCTC 6 cut(s) 597, 825, 1116, 1900, 2169, 2501
AlwI GGATC 2 cut(s) 986, 2437
AlwNI CAGNNNCTG 3 cut(s) 196, 2084, 2173
ApeKI GCWGC 8 cut(s) 190, 286, 692, 695, 698, 1375, 1477, 1918
ApoI RAATTY 4 cut(s) 481, 1011, 2050, 2605
AseI ATTAAT 1 cut(s) 2532
Asp700I GAANNNNTTC 1 cut(s) 2547
Asp718I GGTACC 1 cut(s) 1730
AspA2I CCTAGG 1 cut(s) 839
AspLEI GCGC 4 cut(s) 29, 443, 946, 2417
AspS9I GGNCC 8 cut(s) 51, 1092, 1139, 1166, 1183, 1256, 2129, 2636
AsuC2I CCSGG 3 cut(s) 1370, 1452, 2577
AsuHPI GGTGA 4 cut(s) 446, 856, 1813, 2744
AvaII GGWCC 6 cut(s) 1092, 1166, 1183, 1256, 2129, 2636
AvrII CCTAGG 1 cut(s) 839
AxyI CCTNAGG 1 cut(s) 882
BaeGI GKGCMC 1 cut(s) 264
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BalI TGGCCA 4 cut(s) 384, 1788, 1998, 2139
BanI GGYRCC 4 cut(s) 26, 497, 1397, 1730
BarI GAAGNNNNNNTAC 2 cut(s) 1714, 1746
BauI CACGAG 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 1566
Bbv12I GWGCWC 1 cut(s) 1279
BbvI GCAGC 8 cut(s) 202, 298, 682, 704, 710, 1362, 1489, 1930
BceAI ACGGC 2 cut(s) 2732, 2738
BciT130I CCWGG 6 cut(s) 503, 558, 1116, 1683, 1915, 2380
BciVI GTATCC 3 cut(s) 739, 1349, 1444
BclI TGATCA 1 cut(s) 163
BcnI CCSGG 3 cut(s) 1370, 1452, 2577
BcoDI GTCTC 6 cut(s) 597, 825, 1116, 1900, 2169, 2501
BcuI ACTAGT 1 cut(s) 1958
BfaI CTAG 7 cut(s) 357, 840, 1317, 1353, 1959, 2597, 2755
BfmI CTRYAG 2 cut(s) 696, 2174
BfoI RGCGCY 2 cut(s) 30, 444
BfuAI ACCTGC 2 cut(s) 2086, 2195
BfuI GTATCC 3 cut(s) 739, 1349, 1444
BglI GCCNNNNNGGC 1 cut(s) 2423
BisI GCNGC 9 cut(s) 191, 194, 287, 693, 696, 699, 1376, 1478, 1919
BlnI CCTAGG 1 cut(s) 839
BlsI GCNGC 9 cut(s) 192, 195, 288, 694, 697, 700, 1377, 1479, 1920
BmcAI AGTACT 1 cut(s) 1648
Bme1390I CCNGG 9 cut(s) 503, 558, 1116, 1370, 1452, 1683, 1915, 2380, 2577
Bme18I GGWCC 6 cut(s) 1092, 1166, 1183, 1256, 2129, 2636
BmgT120I GGNCC 8 cut(s) 51, 1092, 1139, 1166, 1183, 1256, 2129, 2636
BmiI GGNNCC 8 cut(s) 28, 52, 499, 1094, 1399, 1489, 1732, 2463
BmrFI CCNGG 9 cut(s) 503, 558, 1116, 1370, 1452, 1683, 1915, 2380, 2577
BmrI ACTGGG 1 cut(s) 1751
BmsI GCATC 6 cut(s) 343, 861, 992, 2030, 2388, 2683
BmuI ACTGGG 1 cut(s) 1751
BpiI GAAGAC 1 cut(s) 1566
BplI GAGNNNNNCTC 2 cut(s) 2663, 2695
BpmI CTGGAG 2 cut(s) 1137, 2665
BpuEI CTTGAG 5 cut(s) 92, 654, 1091, 2625, 2747
BpuMI CCSGG 3 cut(s) 1370, 1452, 2577
BsaBI GATNNNNATC 2 cut(s) 66, 228
BsaHI GRCGYC 1 cut(s) 27
BsaI GGTCTC 3 cut(s) 597, 825, 2501
BsaJI CCNNGG 6 cut(s) 501, 839, 1280, 1797, 2356, 2419
BsaXI ACNNNNNCTCC 2 cut(s) 1110, 1140
Bsc4I CCNNNNNNNGG 9 cut(s) 311, 572, 841, 1102, 1457, 1649, 1763, 2332, 2545
Bse118I RCCGGY 1 cut(s) 2065
Bse1I ACTGG 7 cut(s) 30, 179, 1379, 1654, 1757, 1836, 2682
Bse21I CCTNAGG 1 cut(s) 882
Bse3DI GCAATG 4 cut(s) 46, 266, 954, 1050
Bse8I GATNNNNATC 2 cut(s) 66, 228
BseBI CCWGG 6 cut(s) 503, 558, 1116, 1683, 1915, 2380
BseDI CCNNGG 6 cut(s) 501, 839, 1280, 1797, 2356, 2419
BseJI GATNNNNATC 2 cut(s) 66, 228
BseLI CCNNNNNNNGG 9 cut(s) 311, 572, 841, 1102, 1457, 1649, 1763, 2332, 2545
BseMI GCAATG 4 cut(s) 46, 266, 954, 1050
BseMII CTCAG 6 cut(s) 231, 873, 937, 2180, 2512, 2517
BseNI ACTGG 7 cut(s) 30, 179, 1379, 1654, 1757, 1836, 2682
BseRI GAGGAG 4 cut(s) 624, 675, 1480, 2364
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 8 cut(s) 202, 298, 682, 704, 710, 1362, 1489, 1930
BseYI CCCAGC 2 cut(s) 294, 1136
BsgI GTGCAG 2 cut(s) 846, 2683
Bsh1236I CGCG 1 cut(s) 2415
BshNI GGYRCC 4 cut(s) 26, 497, 1397, 1730
BsiHKAI GWGCWC 1 cut(s) 1279
BsiSI CCGG 5 cut(s) 1369, 1452, 2066, 2443, 2576
BsiWI CGTACG 1 cut(s) 2713
BslFI GGGAC 4 cut(s) 424, 1999, 2681, 2723
BslI CCNNNNNNNGG 9 cut(s) 311, 572, 841, 1102, 1457, 1649, 1763, 2332, 2545
BsmAI GTCTC 6 cut(s) 597, 825, 1116, 1900, 2169, 2501
BsmFI GGGAC 4 cut(s) 424, 1999, 2681, 2723
BsmI GAATGC 3 cut(s) 1138, 2296, 2575
Bso31I GGTCTC 3 cut(s) 597, 825, 2501
Bsp1286I GDGCHC 2 cut(s) 264, 1279
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 7 cut(s) 7, 75, 163, 427, 451, 991, 2429
BspACI CCGC 3 cut(s) 193, 1616, 1928
BspCNI CTCAG 6 cut(s) 232, 874, 936, 2179, 2511, 2518
BspFNI CGCG 1 cut(s) 2415
BspHI TCATGA 1 cut(s) 762
BspLI GGNNCC 8 cut(s) 28, 52, 499, 1094, 1399, 1489, 1732, 2463
BspMAI CTGCAG 2 cut(s) 700, 2178
BspMI ACCTGC 2 cut(s) 2086, 2195
BspPI GGATC 2 cut(s) 986, 2437
BspQI GCTCTTC 1 cut(s) 1783
BspT107I GGYRCC 4 cut(s) 26, 497, 1397, 1730
BspTNI GGTCTC 3 cut(s) 597, 825, 2501
BsrDI GCAATG 4 cut(s) 46, 266, 954, 1050
BsrFI RCCGGY 1 cut(s) 2065
BsrGI TGTACA 1 cut(s) 105
BsrI ACTGG 7 cut(s) 30, 179, 1379, 1654, 1757, 1836, 2682
BssAI RCCGGY 1 cut(s) 2065
BssECI CCNNGG 6 cut(s) 501, 839, 1280, 1797, 2356, 2419
BssMI GATC 7 cut(s) 7, 75, 163, 427, 451, 991, 2429
BssNI GRCGYC 1 cut(s) 27
BssSI CACGAG 1 cut(s) 369
BssT1I CCWWGG 4 cut(s) 839, 1280, 2356, 2419
Bst2BI CACGAG 1 cut(s) 369
Bst2UI CCWGG 6 cut(s) 503, 558, 1116, 1683, 1915, 2380
Bst6I CTCTTC 4 cut(s) 761, 1068, 1203, 1783
BstACI GRCGYC 1 cut(s) 27
BstAPI GCANNNNNTGC 2 cut(s) 268, 2008
BstAUI TGTACA 1 cut(s) 105
BstC8I GCNNGC 5 cut(s) 1143, 1848, 2000, 2067, 2085
BstDSI CCRYGG 1 cut(s) 1797
BstFNI CGCG 1 cut(s) 2415
BstH2I RGCGCY 2 cut(s) 30, 444
BstHHI GCGC 4 cut(s) 29, 443, 946, 2417
BstKTI GATC 7 cut(s) 10, 78, 166, 430, 454, 994, 2432
BstMAI GTCTC 6 cut(s) 597, 825, 1116, 1900, 2169, 2501
BstMBI GATC 7 cut(s) 7, 75, 163, 427, 451, 991, 2429
BstNI CCWGG 6 cut(s) 503, 558, 1116, 1683, 1915, 2380
BstNSI RCATGY 2 cut(s) 338, 1307
BstSCI CCNGG 9 cut(s) 501, 556, 1114, 1368, 1450, 1681, 1913, 2378, 2575
BstSFI CTRYAG 2 cut(s) 696, 2174
BstSLI GKGCMC 1 cut(s) 264
BstUI CGCG 1 cut(s) 2415
BstV1I GCAGC 8 cut(s) 202, 298, 682, 704, 710, 1362, 1489, 1930
BstV2I GAAGAC 1 cut(s) 1566
BstXI CCANNNNNNTGG 1 cut(s) 2363
Bsu36I CCTNAGG 1 cut(s) 882
BsuI GTATCC 3 cut(s) 739, 1349, 1444
BtgI CCRYGG 1 cut(s) 1797
BtsI GCAGTG 2 cut(s) 858, 1279
BtsIMutI CAGTG 9 cut(s) 140, 403, 858, 1279, 1386, 1590, 1843, 2085, 2689
BveI ACCTGC 2 cut(s) 2086, 2195
Cac8I GCNNGC 5 cut(s) 1143, 1848, 2000, 2067, 2085
CaiI CAGNNNCTG 3 cut(s) 196, 2084, 2173
CciI TCATGA 1 cut(s) 762
CfoI GCGC 4 cut(s) 29, 443, 946, 2417
Cfr10I RCCGGY 1 cut(s) 2065
Cfr13I GGNCC 8 cut(s) 51, 1092, 1139, 1166, 1183, 1256, 2129, 2636
CseI GACGC 1 cut(s) 1431
CsiI ACCWGGT 1 cut(s) 1681
Csp6I GTAC 7 cut(s) 106, 176, 315, 1229, 1647, 1731, 2714
CspCI CAANNNNNGTGG 2 cut(s) 723, 758
CviAII CATG 7 cut(s) 335, 763, 908, 932, 1304, 2158, 2591
CviQI GTAC 7 cut(s) 106, 176, 315, 1229, 1647, 1731, 2714
DinI GGCGCC 1 cut(s) 28
DpnI GATC 7 cut(s) 9, 77, 165, 429, 453, 993, 2431
DpnII GATC 7 cut(s) 7, 75, 163, 427, 451, 991, 2429
EaeI YGGCCR 7 cut(s) 382, 1078, 1366, 1786, 1996, 2137, 2444
Eam1104I CTCTTC 4 cut(s) 761, 1068, 1203, 1783
EarI CTCTTC 4 cut(s) 761, 1068, 1203, 1783
Eco130I CCWWGG 4 cut(s) 839, 1280, 2356, 2419
Eco31I GGTCTC 3 cut(s) 597, 825, 2501
Eco32I GATATC 1 cut(s) 390
Eco47I GGWCC 6 cut(s) 1092, 1166, 1183, 1256, 2129, 2636
Eco57I CTGAAG 1 cut(s) 1872
Eco81I CCTNAGG 1 cut(s) 882
EcoO109I RGGNCCY 2 cut(s) 1166, 2129
EcoRI GAATTC 3 cut(s) 481, 2050, 2605
EcoRII CCWGG 6 cut(s) 501, 556, 1114, 1681, 1913, 2378
EcoRV GATATC 1 cut(s) 390
EcoT14I CCWWGG 4 cut(s) 839, 1280, 2356, 2419
EgeI GGCGCC 1 cut(s) 28
EheI GGCGCC 1 cut(s) 28
ErhI CCWWGG 4 cut(s) 839, 1280, 2356, 2419
FaeI CATG 7 cut(s) 338, 766, 911, 935, 1307, 2161, 2594
FaqI GGGAC 4 cut(s) 424, 1999, 2681, 2723
FatI CATG 7 cut(s) 334, 762, 907, 931, 1303, 2157, 2590
FbaI TGATCA 1 cut(s) 163
Fnu4HI GCNGC 9 cut(s) 191, 194, 287, 693, 696, 699, 1376, 1478, 1919
Fsp4HI GCNGC 9 cut(s) 191, 194, 287, 693, 696, 699, 1376, 1478, 1919
FspBI CTAG 7 cut(s) 357, 840, 1317, 1353, 1959, 2597, 2755
GlaI GCGC 4 cut(s) 28, 442, 945, 2416
GluI GCNGC 9 cut(s) 191, 194, 287, 693, 696, 699, 1376, 1478, 1919
GsaI CCCAGC 2 cut(s) 298, 1140
GsuI CTGGAG 2 cut(s) 1137, 2665
HaeII RGCGCY 2 cut(s) 30, 444
HapII CCGG 5 cut(s) 1369, 1452, 2066, 2443, 2576
HgaI GACGC 1 cut(s) 1431
HhaI GCGC 4 cut(s) 29, 443, 946, 2417
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 7 cut(s) 338, 766, 911, 935, 1307, 2161, 2594
Hin6I GCGC 4 cut(s) 27, 441, 944, 2415
HinP1I GCGC 4 cut(s) 27, 441, 944, 2415
HincII GTYRAC 2 cut(s) 604, 2320
HindII GTYRAC 2 cut(s) 604, 2320
HpaII CCGG 5 cut(s) 1369, 1452, 2066, 2443, 2576
HphI GGTGA 4 cut(s) 446, 856, 1813, 2744
Hpy166II GTNNAC 9 cut(s) 176, 537, 604, 847, 1150, 1291, 1590, 1637, 2320
Hpy8I GTNNAC 9 cut(s) 176, 537, 604, 847, 1150, 1291, 1590, 1637, 2320
Hpy99I CGWCG 1 cut(s) 2724
HpyCH4IV ACGT 1 cut(s) 2712
HpySE526I ACGT 1 cut(s) 2712
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 7 cut(s) 338, 766, 911, 935, 1307, 2161, 2594
HspAI GCGC 4 cut(s) 27, 441, 944, 2415
KasI GGCGCC 1 cut(s) 26
KpnI GGTACC 1 cut(s) 1734
KroI GCCGGC 1 cut(s) 2065
KroNI GCCGGC 1 cut(s) 2067
Ksp22I TGATCA 1 cut(s) 163
Kzo9I GATC 7 cut(s) 7, 75, 163, 427, 451, 991, 2429
LguI GCTCTTC 1 cut(s) 1783
LmnI GCTCC 7 cut(s) 775, 980, 1282, 1493, 1831, 1873, 1897
Lsp1109I GCAGC 8 cut(s) 202, 298, 682, 704, 710, 1362, 1489, 1930
LweI GCATC 6 cut(s) 343, 861, 992, 2030, 2388, 2683
MabI ACCWGGT 1 cut(s) 1681
MaeI CTAG 7 cut(s) 357, 840, 1317, 1353, 1959, 2597, 2755
MaeII ACGT 1 cut(s) 2712
MaeIII GTNAC 4 cut(s) 1443, 1674, 2256, 2407
MalI GATC 7 cut(s) 9, 77, 165, 429, 453, 993, 2431
MboI GATC 7 cut(s) 7, 75, 163, 427, 451, 991, 2429
MhlI GDGCHC 2 cut(s) 264, 1279
MlsI TGGCCA 4 cut(s) 384, 1788, 1998, 2139
MluNI TGGCCA 4 cut(s) 384, 1788, 1998, 2139
Mly113I GGCGCC 1 cut(s) 27
MlyI GAGTC 3 cut(s) 89, 1878, 2188
MmeI TCCRAC 4 cut(s) 42, 403, 1741, 2413
Mox20I TGGCCA 4 cut(s) 384, 1788, 1998, 2139
MroNI GCCGGC 1 cut(s) 2065
MroXI GAANNNNTTC 1 cut(s) 2547
MscI TGGCCA 4 cut(s) 384, 1788, 1998, 2139
MseI TTAA 6 cut(s) 84, 218, 417, 719, 1518, 2532
MslI CAYNNNNRTG 3 cut(s) 1302, 1623, 1799
Msp20I TGGCCA 4 cut(s) 384, 1788, 1998, 2139
MspA1I CMGCKG 4 cut(s) 193, 695, 1930, 2617
MspI CCGG 5 cut(s) 1369, 1452, 2066, 2443, 2576
MspR9I CCNGG 9 cut(s) 503, 558, 1116, 1370, 1452, 1683, 1915, 2380, 2577
Mva1269I GAATGC 3 cut(s) 1138, 2296, 2575
MvaI CCWGG 6 cut(s) 503, 558, 1116, 1683, 1915, 2380
MvnI CGCG 1 cut(s) 2415
NaeI GCCGGC 1 cut(s) 2067
NarI GGCGCC 1 cut(s) 27
NciI CCSGG 3 cut(s) 1370, 1452, 2577
NdeII GATC 7 cut(s) 7, 75, 163, 427, 451, 991, 2429
NgoMIV GCCGGC 1 cut(s) 2065
NlaIII CATG 7 cut(s) 338, 766, 911, 935, 1307, 2161, 2594
NlaIV GGNNCC 8 cut(s) 28, 52, 499, 1094, 1399, 1489, 1732, 2463
NmuCI GTSAC 2 cut(s) 1443, 1674
NspI RCATGY 2 cut(s) 338, 1307
OliI CACNNNNGTG 1 cut(s) 1799
PagI TCATGA 1 cut(s) 762
PaqCI CACCTGC 1 cut(s) 2195
PciI ACATGT 1 cut(s) 334
PciSI GCTCTTC 1 cut(s) 1783
PctI GAATGC 3 cut(s) 1138, 2296, 2575
PdiI GCCGGC 1 cut(s) 2067
PdmI GAANNNNTTC 1 cut(s) 2547
PfeI GAWTC 9 cut(s) 62, 1022, 1358, 1600, 1715, 1978, 2275, 2468, 2735
Pfl23II CGTACG 1 cut(s) 2713
PflMI CCANNNNNTGG 3 cut(s) 1649, 2332, 2545
PfoI TCCNGGA 2 cut(s) 1114, 2378
PkrI GCNGC 9 cut(s) 192, 195, 288, 694, 697, 700, 1377, 1479, 1920
PleI GAGTC 3 cut(s) 89, 1878, 2187
PluTI GGCGCC 1 cut(s) 30
PpsI GAGTC 3 cut(s) 89, 1878, 2187
PpuMI RGGWCCY 2 cut(s) 1166, 2129
PscI ACATGT 1 cut(s) 334
PshBI ATTAAT 1 cut(s) 2532
Psp5II RGGWCCY 2 cut(s) 1166, 2129
Psp6I CCWGG 6 cut(s) 501, 556, 1114, 1681, 1913, 2378
PspFI CCCAGC 2 cut(s) 294, 1136
PspGI CCWGG 6 cut(s) 501, 556, 1114, 1681, 1913, 2378
PspLI CGTACG 1 cut(s) 2713
PspN4I GGNNCC 8 cut(s) 28, 52, 499, 1094, 1399, 1489, 1732, 2463
PspPI GGNCC 8 cut(s) 51, 1092, 1139, 1166, 1183, 1256, 2129, 2636
PspPPI RGGWCCY 2 cut(s) 1166, 2129
PstI CTGCAG 2 cut(s) 700, 2178
PstNI CAGNNNCTG 3 cut(s) 196, 2084, 2173
PvuII CAGCTG 2 cut(s) 695, 2617
RsaI GTAC 7 cut(s) 107, 177, 316, 1230, 1648, 1732, 2715
RsaNI GTAC 7 cut(s) 106, 176, 315, 1229, 1647, 1731, 2714
RseI CAYNNNNRTG 3 cut(s) 1302, 1623, 1799
SapI GCTCTTC 1 cut(s) 1783
SaqAI TTAA 6 cut(s) 84, 218, 417, 719, 1518, 2532
SatI GCNGC 9 cut(s) 191, 194, 287, 693, 696, 699, 1376, 1478, 1919
Sau3AI GATC 7 cut(s) 7, 75, 163, 427, 451, 991, 2429
Sau96I GGNCC 8 cut(s) 51, 1092, 1139, 1166, 1183, 1256, 2129, 2636
ScaI AGTACT 1 cut(s) 1648
SchI GAGTC 3 cut(s) 89, 1878, 2188
ScrFI CCNGG 9 cut(s) 503, 558, 1116, 1370, 1452, 1683, 1915, 2380, 2577
SduI GDGCHC 2 cut(s) 264, 1279
SexAI ACCWGGT 1 cut(s) 1681
SfaNI GCATC 6 cut(s) 343, 861, 992, 2030, 2388, 2683
SfcI CTRYAG 2 cut(s) 696, 2174
SfoI GGCGCC 1 cut(s) 28
SinI GGWCC 6 cut(s) 1092, 1166, 1183, 1256, 2129, 2636
SmiMI CAYNNNNRTG 3 cut(s) 1302, 1623, 1799
SmlI CTYRAG 5 cut(s) 71, 669, 1070, 2640, 2726
SmoI CTYRAG 5 cut(s) 71, 669, 1070, 2640, 2726
SpeI ACTAGT 1 cut(s) 1958
SsiI CCGC 3 cut(s) 193, 1616, 1928
SspDI GGCGCC 1 cut(s) 26
SspI AATATT 3 cut(s) 82, 1752, 2700
SspMI CTAG 7 cut(s) 357, 840, 1317, 1353, 1959, 2597, 2755
StyD4I CCNGG 9 cut(s) 501, 556, 1114, 1368, 1450, 1681, 1913, 2378, 2575
StyI CCWWGG 4 cut(s) 839, 1280, 2356, 2419
TaiI ACGT 1 cut(s) 2715
TaqI TCGA 2 cut(s) 1659, 2473
TatI WGTACW 2 cut(s) 105, 1646
TauI GCSGC 1 cut(s) 196
TfiI GAWTC 9 cut(s) 62, 1022, 1358, 1600, 1715, 1978, 2275, 2468, 2735
Tru1I TTAA 6 cut(s) 84, 218, 417, 719, 1518, 2532
Tru9I TTAA 6 cut(s) 84, 218, 417, 719, 1518, 2532
TscAI CASTG 9 cut(s) 147, 403, 865, 1279, 1386, 1597, 1843, 2085, 2689
TseFI GTSAC 2 cut(s) 1443, 1674
TseI GCWGC 8 cut(s) 190, 286, 692, 695, 698, 1375, 1477, 1918
Tsp45I GTSAC 2 cut(s) 1443, 1674
TspDTI ATGAA 8 cut(s) 297, 594, 705, 751, 779, 1956, 2063, 2579
TspGWI ACGGA 1 cut(s) 621
TspRI CASTG 9 cut(s) 147, 403, 865, 1279, 1386, 1597, 1843, 2085, 2689
Van91I CCANNNNNTGG 3 cut(s) 1649, 2332, 2545
VpaK11BI GGWCC 6 cut(s) 1092, 1166, 1183, 1256, 2129, 2636
VspI ATTAAT 1 cut(s) 2532
XapI RAATTY 4 cut(s) 481, 1011, 2050, 2605
XceI RCATGY 2 cut(s) 338, 1307
XmaJI CCTAGG 1 cut(s) 839
XmnI GAANNNNTTC 1 cut(s) 2547
XspI CTAG 7 cut(s) 357, 840, 1317, 1353, 1959, 2597, 2755
ZrmI AGTACT 1 cut(s) 1648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.