FvH4_2g37770

Large proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005387
Physical Location & Seq
Forward (+)
27413754 .. 27420769
7016 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g37770.t5

Sequence Viewer

Length: 2796 bp
ATGGCAAATCAGCACTCAAATGAAGGCTCCGGTCCCAGCAACATTTCTGAGGAGACTTCTGATTCAATTGTTGAGATCAATATCAAGACTTTAGAATCACAGATATATAGTTTTCAAGTGGATAAAAATATGCTAGTGTCCTTGTTCAAAGAACAAATAGCGAATCAAACCGGTGTCCCAGTTGGTCAGCAGAGGTTGATTTTCAGGGGAAAGGTGCTAAAGGATGATCATCCCCTCTCCGAGTATCATTTGGAAAATGGGCATACATTGCACTTGGTTGTGAGGCAGCCATCCCAGCCACAAACTTCATCTGGTACTAGTTCTGCTGAGCCTCATGCTAATACTGGAAATGAGGGCAGTGGGCCTCCTCGTGGTCGCATAGGGCAGGTTTCACACAGTGTAGTTCTTGGGACCTTCAATGTTGGAGATCAAGGTGAAAGCATGGCCCCGGACCTTAGTCGGGTTATTGGCGCGGTTCTGAATTCTATTGGAATCGGCAACCAAGCTGCAACTAATGTTACCAGCAACATACAGTCTACTACGCCAAATAATCCCGGTCAGTCTCCTCAAGGAAATGAAACAGAAGGGTTGCATGGTAATGCAACTAGTCAAAGTCAAGGAGGAAATAATGCGCCGTCCGGACAGACATTTTCTTCTCAGCCATTTCAAACTTTGCCTCAGTTTTTGCAAACTCCCCTTGCTGCTGGAGCTTCTCCCTTTCCTTCACTAAATACGCCCATTCCAGATGCTTTGAACACTCTCTCTGAGTTTATGAACCGCATGGAGCGGGCATTATCACAAAATGGAGGCTACCAACAAAATATATCAGCAACAAACCCAGGAGACCTACCAAGGGTGAACTTACCTTCCAATGCTCTGGGTTTGCCCACTCCTGACGCTTTGGGCATTGTTCTTCGTAATGCAGAACGACTTCTCAGCAGTCATGCTGCTTCTGCACTATCTCACATTGCAGGGCGCTTGGAACAAGAGGGAGCATCTGCTGATCCAGCTGCAAGGGGCCAAATTCAGACAGAATCTATTCAAGTAGGGCTTGCTATGCAACATCTAGGTGCTCTTCTTCTTGAGCTGGGCCGGACAATTTGGACACTCCGCATGGGACAATCTCCTGGAGAAGCTGTTGTGAATGCTGGGCCAGCAGTTTACATATCCCCATCAGGGCCTAATCCCATAATGGTGCAGCCTATTCCTTATCAAGCAAGCTCCCCTTTCAGTGGTTCTGTACCCTTATCGAATCCTATGAATTTTGGTCCTGTTGGCATTGGAAGTGCTCCAAGGAATGTAAACATTCATATACATGCTGGTACCTCACTCTCTGCACTTGGCGCTAGGGGAAGTAACGGGGAAGGGATGCAGGGAGAACATCGTAATGGACCTGGGTCTAGAGATTCAGGTGCAGTACGAGTGCTGCCAGTGAGAAATGTCATTGCTACCACCATTCCATCATCCCAAACTGGTATTTCAATGTCCAGTGCCACTCAGCCTGGTTCAGGTGTATCTGTTCCACAGCCGCCTTCAGACTCCTCTCTTTCATCCATCGTTGCTGAACTTAATTCACAGATAAGAAACTTGGTTGGTAACAATCAAGGAAATGATGCAGTTCAATCAGGTCAAGCAGTGCCCAATGTTCAGAATCCATCTGCTGGCATTGAATCACGTAATAATACAGGGAATGAGCAGCTCAGTAATTCAGATGTTAATGGAGGTCTGCAATCTAATGCATCGCTACCTCGTAGCACATCTGAAAGTGAAGTTCAGAAGGCGTCAGGAAGTGTACCTCCCTTGAAAGATGATTCAAAATTCCAAGCCAGAGATTCTCTTTCAAGTGGACAAATGCCATGTCAAGATGACAAAGGAAATACCTCACAAACTGCTGCCAAACAGGGTATGACCGAGGGTGCAAAAGCTGTTCCTCTTGGATTAGGACTTGGAATGATGGAACGTAAGAGACAAGGCAGGCAGCAGAAGACTCCTCAAGAGAATAGTGATAGCGGAACGACTAGCTCTTCCTCCAATCAGAATCAGCAAGTAACAAGTGCCCAACAACTTTTGCAGTCTCTTGCAACTCGTAGTACTGCTGGCAGTAGGGTGAGCACAATTGATACTCCTGCAAGGCAAGCAGCCCCCAATGTTGGGCAGGTTAGAGATGGTAGATCATCGGGAGTGCAAGGTCCTGGTGGCCAAGTTGATATGGGAAGTGTGATGTCTCAGGTCCTGCAGAGTCCTGCTTTAAATGGTCTGTTGACAGGTGTTTCGGAGCAAACTGGGGTTGGTTCTCCTGATGCACTGAGGAATATGTTGCAGAACTTTACACAGAGTCCACAGATGATGAATGCTGTCAGTCAGATTACTGAACAGGTTGACACCCAAGAATTTGGAAACTTGTTTGCTGGGCTTGGTGGAGGCCAAGGTGGTGGAATTGATATGTCAAGGATGTTCCAACAGATGATGCCCATTGTATCTCGTGCCCTAGGGGCAGGGTCTTCACCCGCTCAGCCACTTCCTGTCATGGGAACTGAATCACATTCACTGTACAACGAGAGGAATCTCAACAGAGATGATAATGAGATTGACCTCCAACAAGTGGTTCAAAGGATTGAGCAGCTGAATTCACCCGAAGATGTTTTCCAAGCTGTAGTTGAAAATTCAGTTCAGCTATCTGGCAGAGGAAGCAGTCCACGAGAACTTGTGGATGAGTTGTGCAGGGACGAGGGTCTCTCCAGTGAATATGCTGAGATCTTACGGACGGATATACGTCAAAGACTTGAGGGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001822 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010605 GO:0010941 GO:0012501 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031593 GO:0031647 GO:0032268 GO:0032269 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042981 GO:0043021 GO:0043022 GO:0043067 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045861 GO:0045995 GO:0048232 GO:0048285 GO:0048513 GO:0048519 GO:0048523 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070059 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1903046 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

932

Amino Acids

97.43

Weight (kDa)

5.22

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 25 - 94 3.6e-08 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 26 - 96 1.3e-22 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 376, 2146
Acc65I GGTACC 1 cut(s) 1322
AccB1I GGYRCC 1 cut(s) 1322
AccB7I CCANNNNNTGG 2 cut(s) 1661, 2602
AccBSI CCGCTC 2 cut(s) 787, 2510
AccI GTMKAC 1 cut(s) 536
AccII CGCG 1 cut(s) 473
AccIII TCCGGA 1 cut(s) 638
AciI CCGC 7 cut(s) 473, 778, 787, 1111, 1529, 2010, 2508
AclWI GGATC 1 cut(s) 998
AcoI YGGCCR 1 cut(s) 2197
AcsI RAATTY 7 cut(s) 481, 1023, 1261, 1817, 2390, 2626, 2662
AcuI CTGAAG 1 cut(s) 1518
AcyI GRCGYC 1 cut(s) 1781
AdeI CACNNNGTG 1 cut(s) 398
AfaI GTAC 7 cut(s) 316, 1242, 1324, 1419, 1794, 2092, 2552
AgeI ACCGGT 1 cut(s) 170
AhlI ACTAGT 2 cut(s) 317, 605
AjnI CCWGG 5 cut(s) 838, 1126, 1393, 1501, 2191
AjuI GAANNNNNNNTTGG 2 cut(s) 2589, 2621
AloI GAACNNNNNNTCC 2 cut(s) 851, 883
Alw21I GWGCWC 3 cut(s) 1075, 1291, 2114
Alw26I GTCTC 7 cut(s) 47, 567, 837, 1960, 2079, 2229, 2738
AlwI GGATC 1 cut(s) 998
AlwNI CAGNNNCTG 1 cut(s) 2233
Aor13HI TCCGGA 1 cut(s) 638
AoxI GGCC 8 cut(s) 362, 444, 1018, 1090, 1151, 1178, 2197, 2422
ApoI RAATTY 7 cut(s) 481, 1023, 1261, 1817, 2390, 2626, 2662
ArsI GACNNNNNNTTYG 4 cut(s) 1843, 1875, 2254, 2286
AsiGI ACCGGT 1 cut(s) 170
Asp700I GAANNNNTTC 2 cut(s) 930, 1038
Asp718I GGTACC 1 cut(s) 1322
AspA2I CCTAGG 1 cut(s) 2488
AspLEI GCGC 4 cut(s) 473, 634, 978, 1346
AsuC2I CCSGG 2 cut(s) 449, 555
AsuHPI GGTGA 5 cut(s) 446, 868, 2119, 2496, 2622
AvaII GGWCC 7 cut(s) 32, 411, 451, 1268, 1391, 2189, 2230
AvrII CCTAGG 1 cut(s) 2488
BaeGI GKGCMC 3 cut(s) 1641, 2059, 2488
BalI TGGCCA 1 cut(s) 2199
BanI GGYRCC 1 cut(s) 1322
BauI CACGAG 3 cut(s) 369, 2481, 2697
BbsI GAAGAC 2 cut(s) 1991, 2493
Bbv12I GWGCWC 3 cut(s) 1075, 1291, 2114
BccI CCATC 7 cut(s) 298, 1180, 1468, 1562, 1663, 1948, 2159
BceAI ACGGC 1 cut(s) 619
BciT130I CCWGG 5 cut(s) 840, 1128, 1395, 1503, 2193
BclI TGATCA 1 cut(s) 226
BcnI CCSGG 2 cut(s) 449, 555
BcoDI GTCTC 7 cut(s) 47, 567, 837, 1960, 2079, 2229, 2738
BcuI ACTAGT 2 cut(s) 317, 605
BfaI CTAG 8 cut(s) 134, 318, 606, 1067, 1347, 1401, 2019, 2489
BfmI CTRYAG 2 cut(s) 2234, 2652
BfoI RGCGCY 2 cut(s) 979, 1347
BfuAI ACCTGC 2 cut(s) 376, 2146
BglI GCCNNNNNGGC 1 cut(s) 2492
BglII AGATCT 1 cut(s) 2754
BlnI CCTAGG 1 cut(s) 2488
BlpI GCTNAGC 2 cut(s) 327, 2511
BmcAI AGTACT 1 cut(s) 2092
Bme1390I CCNGG 7 cut(s) 449, 555, 840, 1128, 1395, 1503, 2193
Bme18I GGWCC 7 cut(s) 32, 411, 451, 1268, 1391, 2189, 2230
BmiI GGNNCC 6 cut(s) 28, 34, 412, 447, 1019, 1324
BmrFI CCNGG 7 cut(s) 449, 555, 840, 1128, 1395, 1503, 2193
BmrI ACTGGG 2 cut(s) 173, 2292
BmsI GCATC 7 cut(s) 736, 1004, 1359, 1603, 1748, 2290, 2457
BmuI ACTGGG 2 cut(s) 173, 2292
BoxI GACNNNNGTC 3 cut(s) 456, 1396, 2730
BpiI GAAGAC 2 cut(s) 1991, 2493
BplI GAGNNNNNCTC 2 cut(s) 2720, 2752
BpmI CTGGAG 3 cut(s) 726, 1149, 2722
Bpu1102I GCTNAGC 2 cut(s) 327, 2511
BpuEI CTTGAG 3 cut(s) 552, 1103, 1977
BpuMI CCSGG 2 cut(s) 449, 555
BsaAI YACGTR 1 cut(s) 1676
BsaBI GATNNNNATC 2 cut(s) 80, 228
BsaHI GRCGYC 1 cut(s) 1781
BsaI GGTCTC 2 cut(s) 837, 2738
BsaJI CCNNGG 8 cut(s) 447, 838, 851, 1292, 1394, 1911, 2425, 2488
BsaWI WCCGGW 3 cut(s) 29, 170, 638
BsaXI ACNNNNNCTCC 2 cut(s) 1122, 1152
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 7 cut(s) 179, 349, 1430, 1477, 1488, 2287, 2739
Bse3DI GCAATG 3 cut(s) 266, 966, 1443
Bse8I GATNNNNATC 2 cut(s) 80, 228
BseAI TCCGGA 1 cut(s) 638
BseBI CCWGG 5 cut(s) 840, 1128, 1395, 1503, 2193
BseDI CCNNGG 8 cut(s) 447, 838, 851, 1292, 1394, 1911, 2425, 2488
BseGI GGATG 8 cut(s) 229, 229, 290, 1374, 1463, 1550, 2457, 2716
BseJI GATNNNNATC 2 cut(s) 80, 228
BseMI GCAATG 3 cut(s) 266, 966, 1443
BseNI ACTGG 7 cut(s) 179, 349, 1430, 1477, 1488, 2287, 2739
BseRI GAGGAG 5 cut(s) 65, 357, 555, 1531, 1980
BseSI GKGCMC 3 cut(s) 1641, 2059, 2488
BseYI CCCAGC 5 cut(s) 35, 294, 1087, 1148, 2408
BsgI GTGCAG 5 cut(s) 939, 1217, 1320, 1434, 2740
Bsh1236I CGCG 1 cut(s) 473
BshFI GGCC 8 cut(s) 364, 446, 1020, 1092, 1153, 1180, 2199, 2424
BshNI GGYRCC 1 cut(s) 1322
BshTI ACCGGT 1 cut(s) 170
BsiHKAI GWGCWC 3 cut(s) 1075, 1291, 2114
BsiSI CCGG 6 cut(s) 30, 171, 449, 555, 639, 1093
BslFI GGGAC 5 cut(s) 18, 161, 424, 1131, 2738
BsmAI GTCTC 7 cut(s) 47, 567, 837, 1960, 2079, 2229, 2738
BsmFI GGGAC 5 cut(s) 18, 161, 424, 1131, 2738
BsmI GAATGC 2 cut(s) 1150, 2356
BsnI GGCC 8 cut(s) 364, 446, 1020, 1092, 1153, 1180, 2199, 2424
Bso31I GGTCTC 2 cut(s) 837, 2738
Bsp1286I GDGCHC 6 cut(s) 1075, 1291, 1641, 2059, 2114, 2488
Bsp13I TCCGGA 1 cut(s) 638
Bsp1407I TGTACA 1 cut(s) 2550
Bsp143I GATC 6 cut(s) 75, 226, 427, 1003, 2171, 2754
Bsp1720I GCTNAGC 2 cut(s) 327, 2511
BspACI CCGC 7 cut(s) 473, 778, 787, 1111, 1529, 2010, 2508
BspANI GGCC 8 cut(s) 364, 446, 1020, 1092, 1153, 1180, 2199, 2424
BspEI TCCGGA 1 cut(s) 638
BspFNI CGCG 1 cut(s) 473
BspLI GGNNCC 6 cut(s) 28, 34, 412, 447, 1019, 1324
BspMAI CTGCAG 1 cut(s) 2238
BspMI ACCTGC 2 cut(s) 376, 2146
BspPI GGATC 1 cut(s) 998
BspQI GCTCTTC 2 cut(s) 1080, 2029
BspT107I GGYRCC 1 cut(s) 1322
BspTNI GGTCTC 2 cut(s) 837, 2738
BsrBI CCGCTC 2 cut(s) 787, 2510
BsrDI GCAATG 3 cut(s) 266, 966, 1443
BsrFI RCCGGY 1 cut(s) 170
BsrGI TGTACA 1 cut(s) 2550
BsrI ACTGG 7 cut(s) 179, 349, 1430, 1477, 1488, 2287, 2739
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 8 cut(s) 447, 838, 851, 1292, 1394, 1911, 2425, 2488
BssMI GATC 6 cut(s) 75, 226, 427, 1003, 2171, 2754
BssNI GRCGYC 1 cut(s) 1781
BssSI CACGAG 3 cut(s) 369, 2481, 2697
BssT1I CCWWGG 4 cut(s) 851, 1292, 2425, 2488
Bst2BI CACGAG 3 cut(s) 369, 2481, 2697
Bst2UI CCWGG 5 cut(s) 840, 1128, 1395, 1503, 2193
Bst4CI ACNGT 3 cut(s) 398, 534, 2550
Bst6I CTCTTC 2 cut(s) 1080, 2029
BstACI GRCGYC 1 cut(s) 1781
BstAPI GCANNNNNTGC 1 cut(s) 268
BstAUI TGTACA 1 cut(s) 2550
BstBAI YACGTR 1 cut(s) 1676
BstC8I GCNNGC 8 cut(s) 789, 1053, 1155, 1219, 1663, 1976, 2098, 2136
BstENI CCTNNNNNAGG 1 cut(s) 1506
BstF5I GGATG 8 cut(s) 229, 229, 290, 1374, 1463, 1550, 2457, 2716
BstFNI CGCG 1 cut(s) 473
BstH2I RGCGCY 2 cut(s) 979, 1347
BstHHI GCGC 4 cut(s) 473, 634, 978, 1346
BstKTI GATC 6 cut(s) 78, 229, 430, 1006, 2174, 2757
BstMAI GTCTC 7 cut(s) 47, 567, 837, 1960, 2079, 2229, 2738
BstMBI GATC 6 cut(s) 75, 226, 427, 1003, 2171, 2754
BstNI CCWGG 5 cut(s) 840, 1128, 1395, 1503, 2193
BstNSI RCATGY 1 cut(s) 1319
BstPAI GACNNNNGTC 3 cut(s) 456, 1396, 2730
BstSCI CCNGG 7 cut(s) 447, 553, 838, 1126, 1393, 1501, 2191
BstSFI CTRYAG 2 cut(s) 2234, 2652
BstSLI GKGCMC 3 cut(s) 1641, 2059, 2488
BstUI CGCG 1 cut(s) 473
BstV2I GAAGAC 2 cut(s) 1991, 2493
BstX2I RGATCY 1 cut(s) 2754
BstXI CCANNNNNNTGG 3 cut(s) 877, 2393, 2432
BstYI RGATCY 1 cut(s) 2754
BsuRI GGCC 8 cut(s) 364, 446, 1020, 1092, 1153, 1180, 2199, 2424
BtgZI GCGATG 1 cut(s) 1725
BtsCI GGATG 8 cut(s) 229, 229, 290, 1374, 1463, 1550, 2457, 2716
BtsI GCAGTG 2 cut(s) 364, 1641
BtsIMutI CAGTG 9 cut(s) 364, 403, 1237, 1437, 1495, 1641, 2303, 2546, 2746
BveI ACCTGC 2 cut(s) 376, 2146
Cac8I GCNNGC 8 cut(s) 789, 1053, 1155, 1219, 1663, 1976, 2098, 2136
CaiI CAGNNNCTG 1 cut(s) 2233
CfoI GCGC 4 cut(s) 473, 634, 978, 1346
Cfr10I RCCGGY 1 cut(s) 170
CseI GACGC 2 cut(s) 905, 1770
Csp6I GTAC 7 cut(s) 315, 1241, 1323, 1418, 1793, 2091, 2551
CspAI ACCGGT 1 cut(s) 170
CviAII CATG 9 cut(s) 335, 442, 593, 781, 944, 1114, 1316, 1857, 2527
CviQI GTAC 7 cut(s) 315, 1241, 1323, 1418, 1793, 2091, 2551
DpnI GATC 6 cut(s) 77, 228, 429, 1005, 2173, 2756
DpnII GATC 6 cut(s) 75, 226, 427, 1003, 2171, 2754
DraI TTTAAA 1 cut(s) 2250
DraIII CACNNNGTG 1 cut(s) 398
EaeI YGGCCR 1 cut(s) 2197
Eam1104I CTCTTC 2 cut(s) 1080, 2029
EarI CTCTTC 2 cut(s) 1080, 2029
Eco130I CCWWGG 4 cut(s) 851, 1292, 2425, 2488
Eco31I GGTCTC 2 cut(s) 837, 2738
Eco47I GGWCC 7 cut(s) 32, 411, 451, 1268, 1391, 2189, 2230
Eco57I CTGAAG 1 cut(s) 1518
EcoNI CCTNNNNNAGG 1 cut(s) 1506
EcoO109I RGGNCCY 4 cut(s) 411, 1178, 2189, 2230
EcoRI GAATTC 2 cut(s) 481, 2626
EcoRII CCWGG 5 cut(s) 838, 1126, 1393, 1501, 2191
EcoT14I CCWWGG 4 cut(s) 851, 1292, 2425, 2488
EcoT22I ATGCAT 1 cut(s) 1741
ErhI CCWWGG 4 cut(s) 851, 1292, 2425, 2488
FaeI CATG 9 cut(s) 338, 445, 596, 784, 947, 1117, 1319, 1860, 2530
FalI AAGNNNNNCTT 4 cut(s) 1035, 1067, 1210, 1242
FaqI GGGAC 5 cut(s) 18, 161, 424, 1131, 2738
FatI CATG 9 cut(s) 334, 441, 592, 780, 943, 1113, 1315, 1856, 2526
FauI CCCGC 2 cut(s) 780, 2515
FbaI TGATCA 1 cut(s) 226
FblI GTMKAC 1 cut(s) 536
FokI GGATG 8 cut(s) 216, 236, 277, 1381, 1450, 1537, 2464, 2723
FspBI CTAG 8 cut(s) 134, 318, 606, 1067, 1347, 1401, 2019, 2489
GlaI GCGC 4 cut(s) 472, 633, 977, 1345
GsaI CCCAGC 5 cut(s) 39, 298, 1091, 1152, 2412
GsuI CTGGAG 3 cut(s) 726, 1149, 2722
HaeII RGCGCY 2 cut(s) 979, 1347
HaeIII GGCC 8 cut(s) 364, 446, 1020, 1092, 1153, 1180, 2199, 2424
HapII CCGG 6 cut(s) 30, 171, 449, 555, 639, 1093
HgaI GACGC 2 cut(s) 905, 1770
HhaI GCGC 4 cut(s) 473, 634, 978, 1346
Hin1I GRCGYC 1 cut(s) 1781
Hin1II CATG 9 cut(s) 338, 445, 596, 784, 947, 1117, 1319, 1860, 2530
Hin6I GCGC 4 cut(s) 471, 632, 976, 1344
HinP1I GCGC 4 cut(s) 471, 632, 976, 1344
HincII GTYRAC 2 cut(s) 2262, 2380
HindII GTYRAC 2 cut(s) 2262, 2380
HpaII CCGG 6 cut(s) 30, 171, 449, 555, 639, 1093
HphI GGTGA 5 cut(s) 446, 868, 2119, 2496, 2622
HpyAV CCTTC 8 cut(s) 17, 424, 578, 732, 876, 1358, 1542, 1771
HpyCH4III ACNGT 3 cut(s) 398, 534, 2550
HpyCH4IV ACGT 3 cut(s) 1675, 1960, 2773
HpySE526I ACGT 3 cut(s) 1675, 1960, 2773
Hsp92I GRCGYC 1 cut(s) 1781
Hsp92II CATG 9 cut(s) 338, 445, 596, 784, 947, 1117, 1319, 1860, 2530
HspAI GCGC 4 cut(s) 471, 632, 976, 1344
Kpn2I TCCGGA 1 cut(s) 638
KpnI GGTACC 1 cut(s) 1326
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 75, 226, 427, 1003, 2171, 2754
LguI GCTCTTC 2 cut(s) 1080, 2029
LmnI GCTCC 7 cut(s) 32, 707, 784, 992, 1226, 1294, 2275
LweI GCATC 7 cut(s) 736, 1004, 1359, 1603, 1748, 2290, 2457
MaeI CTAG 8 cut(s) 134, 318, 606, 1067, 1347, 1401, 2019, 2489
MaeII ACGT 3 cut(s) 1675, 1960, 2773
MaeIII GTNAC 4 cut(s) 517, 1355, 1595, 2047
MalI GATC 6 cut(s) 77, 228, 429, 1005, 2173, 2756
MbiI CCGCTC 2 cut(s) 787, 2510
MboI GATC 6 cut(s) 75, 226, 427, 1003, 2171, 2754
MboII GAAGA 8 cut(s) 645, 905, 1067, 1070, 1996, 2016, 2493, 2648
MfeI CAATTG 2 cut(s) 66, 2115
MflI RGATCY 1 cut(s) 2754
MhlI GDGCHC 6 cut(s) 1075, 1291, 1641, 2059, 2114, 2488
MlsI TGGCCA 1 cut(s) 2199
MluNI TGGCCA 1 cut(s) 2199
MlyI GAGTC 4 cut(s) 1532, 1981, 2248, 2344
MmeI TCCRAC 3 cut(s) 403, 2482, 2620
Mox20I TGGCCA 1 cut(s) 2199
Mph1103I ATGCAT 1 cut(s) 1741
MroI TCCGGA 1 cut(s) 638
MroXI GAANNNNTTC 2 cut(s) 930, 1038
MscI TGGCCA 1 cut(s) 2199
MseI TTAA 3 cut(s) 1569, 1716, 2249
MslI CAYNNNNRTG 6 cut(s) 18, 597, 1068, 1193, 1314, 1386
Msp20I TGGCCA 1 cut(s) 2199
MspA1I CMGCKG 2 cut(s) 1010, 2623
MspI CCGG 6 cut(s) 30, 171, 449, 555, 639, 1093
MspR9I CCNGG 7 cut(s) 449, 555, 840, 1128, 1395, 1503, 2193
MunI CAATTG 2 cut(s) 66, 2115
Mva1269I GAATGC 2 cut(s) 1150, 2356
MvaI CCWGG 5 cut(s) 840, 1128, 1395, 1503, 2193
MvnI CGCG 1 cut(s) 473
NciI CCSGG 2 cut(s) 449, 555
NdeII GATC 6 cut(s) 75, 226, 427, 1003, 2171, 2754
NlaIII CATG 9 cut(s) 338, 445, 596, 784, 947, 1117, 1319, 1860, 2530
NlaIV GGNNCC 6 cut(s) 28, 34, 412, 447, 1019, 1324
NsiI ATGCAT 1 cut(s) 1741
NspI RCATGY 1 cut(s) 1319
PciSI GCTCTTC 2 cut(s) 1080, 2029
PctI GAATGC 2 cut(s) 1150, 2356
PdmI GAANNNNTTC 2 cut(s) 930, 1038
PflMI CCANNNNNTGG 2 cut(s) 1661, 2602
PfoI TCCNGGA 1 cut(s) 1126
PinAI ACCGGT 1 cut(s) 170
PleI GAGTC 4 cut(s) 1532, 1981, 2247, 2343
PpsI GAGTC 4 cut(s) 1532, 1981, 2247, 2343
Ppu21I YACGTR 1 cut(s) 1676
PpuMI RGGWCCY 3 cut(s) 411, 2189, 2230
PshAI GACNNNNGTC 3 cut(s) 456, 1396, 2730
Psp5II RGGWCCY 3 cut(s) 411, 2189, 2230
Psp6I CCWGG 5 cut(s) 838, 1126, 1393, 1501, 2191
PspFI CCCAGC 5 cut(s) 35, 294, 1087, 1148, 2408
PspGI CCWGG 5 cut(s) 838, 1126, 1393, 1501, 2191
PspN4I GGNNCC 6 cut(s) 28, 34, 412, 447, 1019, 1324
PspPPI RGGWCCY 3 cut(s) 411, 2189, 2230
PstI CTGCAG 1 cut(s) 2238
PstNI CAGNNNCTG 1 cut(s) 2233
PsuI RGATCY 1 cut(s) 2754
PvuII CAGCTG 2 cut(s) 1010, 2623
RsaI GTAC 7 cut(s) 316, 1242, 1324, 1419, 1794, 2092, 2552
RsaNI GTAC 7 cut(s) 315, 1241, 1323, 1418, 1793, 2091, 2551
RseI CAYNNNNRTG 6 cut(s) 18, 597, 1068, 1193, 1314, 1386
SapI GCTCTTC 2 cut(s) 1080, 2029
SaqAI TTAA 3 cut(s) 1569, 1716, 2249
Sau3AI GATC 6 cut(s) 75, 226, 427, 1003, 2171, 2754
ScaI AGTACT 1 cut(s) 2092
SchI GAGTC 4 cut(s) 1532, 1981, 2248, 2344
ScrFI CCNGG 7 cut(s) 449, 555, 840, 1128, 1395, 1503, 2193
SduI GDGCHC 6 cut(s) 1075, 1291, 1641, 2059, 2114, 2488
SfaNI GCATC 7 cut(s) 736, 1004, 1359, 1603, 1748, 2290, 2457
SfcI CTRYAG 2 cut(s) 2234, 2652
SinI GGWCC 7 cut(s) 32, 411, 451, 1268, 1391, 2189, 2230
SmiMI CAYNNNNRTG 6 cut(s) 18, 597, 1068, 1193, 1314, 1386
SmlI CTYRAG 4 cut(s) 567, 1082, 1992, 2783
SmoI CTYRAG 4 cut(s) 567, 1082, 1992, 2783
SpeI ACTAGT 2 cut(s) 317, 605
SsiI CCGC 7 cut(s) 473, 778, 787, 1111, 1529, 2010, 2508
SspMI CTAG 8 cut(s) 134, 318, 606, 1067, 1347, 1401, 2019, 2489
StyD4I CCNGG 7 cut(s) 447, 553, 838, 1126, 1393, 1501, 2191
StyI CCWWGG 4 cut(s) 851, 1292, 2425, 2488
TaaI ACNGT 3 cut(s) 398, 534, 2550
TaiI ACGT 3 cut(s) 1678, 1963, 2776
TaqI TCGA 1 cut(s) 1250
TaqII GACCGA 1 cut(s) 1925
TatI WGTACW 2 cut(s) 2090, 2550
TauI GCSGC 1 cut(s) 1531
Tru1I TTAA 3 cut(s) 1569, 1716, 2249
Tru9I TTAA 3 cut(s) 1569, 1716, 2249
TscAI CASTG 9 cut(s) 364, 403, 1237, 1437, 1495, 1641, 2310, 2553, 2746
TspDTI ATGAA 8 cut(s) 36, 297, 591, 788, 1274, 1298, 1539, 2363
TspGWI ACGGA 2 cut(s) 2776, 2780
TspRI CASTG 9 cut(s) 364, 403, 1237, 1437, 1495, 1641, 2310, 2553, 2746
Van91I CCANNNNNTGG 2 cut(s) 1661, 2602
VpaK11BI GGWCC 7 cut(s) 32, 411, 451, 1268, 1391, 2189, 2230
XagI CCTNNNNNAGG 1 cut(s) 1506
XapI RAATTY 7 cut(s) 481, 1023, 1261, 1817, 2390, 2626, 2662
XbaI TCTAGA 1 cut(s) 1400
XceI RCATGY 1 cut(s) 1319
XmaJI CCTAGG 1 cut(s) 2488
XmiI GTMKAC 1 cut(s) 536
XmnI GAANNNNTTC 2 cut(s) 930, 1038
XspI CTAG 8 cut(s) 134, 318, 606, 1067, 1347, 1401, 2019, 2489
ZrmI AGTACT 1 cut(s) 2092
Zsp2I ATGCAT 1 cut(s) 1741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.