Rroxscaffold_7G00164220

Large proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005387
Physical Location & Seq
Forward (+)
6240366 .. 6247863
7498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00164220.1

Sequence Viewer

Length: 2814 bp
ATGGCAAATCAGTACTCAAATGAAGGCTCTGGTCCCAGCAATGTTTCTGAGGAGACTTCTGATTCAACTGTTGAGATCAATATCAAGACTTTAGAGTCGCAGATATACAGTTTTCAAGTGGAAAAAAATATGCTAGTGTCATTGTTCAAAGAAAAAATAGCTGATCAAACAGGCGTCCCAGTTGGTCAGCAGCGGTTGATTTTCAGGGGAAAGGTGCTCAAGGATGATCATCCCCTTTCCGAGTATCATTTGGAAAATGGGCATACATTGCACTTAGTTGTGAGGCAGCCATCTCAGTCACAAACTTCATCCGGTACTAGTTCTGCTGAGCCTAATGCTAATGCTGGAAATGAGGGTAATGGGGCTCCTCGTGGTCGCATAGGGCAGATTTCACACAGTGTGGTTCTTGGGACCTTCAATGTTGGAGATCAAGGTGAAGGCATGGCCCCAGACCTTGGTCGGGTCATTGGCGCAGTTTTGAATTCTATTGGAATTGGCAACCAAGCTGCAACTAATGTTAATGGCAACATGCAGTCTACTATGCCAAATAATCCTGGTCAGTCTCCTCAAGGAAACGAAACAGAAGGGTTGCATGGTAATGCAAATAGTCAGACTCGAGGAGGAAATAATGCACAGTCTGGGCTGACATTTTCTTCTCAGCCTTTTCAAGCTTTGCCTCAATTTCTGCAAACTCCCCTTGCTGCTGGAGCATCTCCCTTTCCTTCACTAAATTCGCCAATTCCGGACGCTTTGAACACTCTCTCTGAGTTTATGGACCGCATGGAGCAGGCATTATCACAAAATGGAGGCTACCAACAAAATATATCAGCAACAAACCCAGGAGACCTATCAAGGGTGAATTTACCTTCCAATGCTCAGGGGTTGCCCACGCCTGAAGCCTTGGGCATTGTTCTTCGCAATGCAGAACGACTTCTCAGCAGTCATGCTGCTTCTGCACTATCTCACATTGCAGGGCGCTTGGAACAAGAGGGAGCATCTTCTGATCCCTCTGCAAGAGGCCAAATTCAGACAGAATCTATTCAAGTAGGGCTTGCAATGCAACATCTAGGTGCTCTTCTTCTTGAGCTGGGCCGTACAATTTGGACACTCCGCATGGGACAATCTCCTGGAGAAGCTGTTGTGAATGCTGGGCCAGCAGTTTATATTTCCCCGTCGGGGCCTAATCCCATAATGGTGCAGCCTATTCCTTATCAAACAAGCTCCCCTTTTAGTGCTTCTGTACCCCTGTCGAATCCTATGAATTTTGGTCCTGTTGGCATTGGAAATGCTCCAAGGAATGTAAACATTCATATACATGCTGGTACCTCACTCTCTGCACTTGGTGCTAGGGGAAGTAATGGGGAAGGGATGCAGGGAGAACATCGTAATGGACCTGGGTCAAGGGATTCAGGTGCAGCTCGAGTGCTGCCAGTGAGAAATATCATTGCTACCGCTATCCCATCATCCCAAACTGGTATGGCAACTTCCAGTGCATCACAGCCTGGTTCAGGTGTATCTGTACCACGGCCACGTTCAGACTCCTCTCTTTCGTCCATCGTTGCTGAACTTAATTCACAGATTAGAAACTTCGTTGGTAATGCTCAAGGAAATGATGCTGTTCAATCAGGTCAAGCAGTGCCCAATGTGCAGAATTCATCTGCTGGAGTTGGATCACGTAATAATACAGGGAATGAGCAGTCCAGTAATTCAGATGTGAACGGAGATCAGCAATCTAATGCGTCACTACCTGGTTCCACATCTGAAAGTGAAGTTCAGAAGGCTTCAGGAAGTGTACCTCCCTTGAGAGATGATTCAAGATGCCAAGCCAGAGGTTCTCTTTCAAGTGGACAAAATCCAATGCCGTCTGAAGATGACAAAGGAAATGCTTCACAAACTGCTGCAAAACAGGATAAGACCGAGGGCGCAAAAGCTGTTCCACTTGGATTAGGACTTGGAATGTTGGAACGTAAGAGACAAGGCAGGCAGCAAAAGACTCTTCAGAAGAATGGTGATGGTGGAACAACTAGCGCTTCTTCCAATCAGAATCAGCAAGTAACAAGTGGCCAACAACTTTTGCAGTCTCTTGCAACTCGTAGTACTGCTGGAAGTAGGTTGAGCACAAGTGATACTCCTGCAAGGCAAACAGTCCCCAATTTTGGGCAGGTTAGAGATGGTAGATCATTGGGAGGGCAAGGTCCTGGTGGCCAAGTTGATATGGGAAGTGTGATGTCTCAGGTCCTGCAGAGTCCTGCCTTAAATGGTCTGTTGTCAGGTGTTTCGGAGCAAACTGGGGTGGGTTCTCCTGATGCACTGCGGAATATGTTGCAGAACTTTACACAGAGTCCGCAGATGATGAATGCTGTCAACCAGATTACTGAACAGGTTGACACCCAAGATTTCGGAAACTTGTTTGCTGGGCTTGGAGGAGGCCAAGGTGGTGGAATTGATATGTCGAGGATGTTCCAACAGATGATGCCCATTGTATCTCGTGCCCTAGGGGCAGGGTCTTCTCCCGCTCAGCCACCCCCTGTCATGGGATCTCAGTCACTGTACAACGAGAGGAGTCTCAACAGAGATGATAGTGAGATTGACCTCCAACAAGTGGTTCAAAGGATTGGGCAGCTGAATGCACCGGGAGACGTTTTTCAAGCTGTAGTTGAAAATTCAGTTCAGCTATCTGGCAGAGGAAGCAGTCCACAAGAACTTGTGGATGAGTTGTGCAGGGACGAGGGTCTCTCCAGTGAATACGCTGAGATCTTACGGAGGGACATACGTCGAAGACTTGAGGGTGAATCGGGGCAGGACAAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000280 GO:0001655 GO:0001822 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006996 GO:0007029 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007130 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007417 GO:0007420 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010605 GO:0010941 GO:0012501 GO:0016043 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019222 GO:0019538 GO:0019941 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023052 GO:0030162 GO:0030163 GO:0030323 GO:0030324 GO:0030433 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031593 GO:0031647 GO:0032268 GO:0032269 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034613 GO:0034976 GO:0035295 GO:0035556 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0042176 GO:0042177 GO:0042221 GO:0042771 GO:0042981 GO:0043021 GO:0043022 GO:0043067 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0044703 GO:0044877 GO:0045048 GO:0045132 GO:0045143 GO:0045184 GO:0045861 GO:0045995 GO:0048232 GO:0048285 GO:0048513 GO:0048519 GO:0048523 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050821 GO:0050896 GO:0051171 GO:0051172 GO:0051179 GO:0051205 GO:0051234 GO:0051239 GO:0051246 GO:0051248 GO:0051276 GO:0051321 GO:0051603 GO:0051641 GO:0051704 GO:0051716 GO:0051788 GO:0060255 GO:0060322 GO:0060541 GO:0061024 GO:0061136 GO:0061857 GO:0061982 GO:0065007 GO:0065008 GO:0070059 GO:0070192 GO:0070193 GO:0070628 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0071816 GO:0071818 GO:0071840 GO:0072001 GO:0072331 GO:0072332 GO:0072379 GO:0072657 GO:0080090 GO:0090150 GO:0097190 GO:0097193 GO:0098813 GO:0140013 GO:0140030 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901799 GO:1903046 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:2000026 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

937

Amino Acids

97.7

Weight (kDa)

5.37

Isoelectric Point (pI)

51.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 25 - 94 5.7e-08 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 26 - 96 1e-22 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 94
Acc36I ACCTGC 1 cut(s) 2152
Acc65I GGTACC 1 cut(s) 1322
AccB1I GGYRCC 1 cut(s) 1322
AccB7I CCANNNNNTGG 2 cut(s) 455, 2602
AccBSI CCGCTC 1 cut(s) 2516
AccI GTMKAC 1 cut(s) 536
AccIII TCCGGA 1 cut(s) 742
AciI CCGC 7 cut(s) 193, 778, 1111, 1452, 2314, 2345, 2514
AclWI GGATC 3 cut(s) 998, 1678, 2545
AcoI YGGCCR 3 cut(s) 1526, 2062, 2203
AcsI RAATTY 7 cut(s) 481, 730, 859, 1023, 1261, 1651, 2662
AcuI CTGAAG 4 cut(s) 915, 1767, 1887, 1982
AcyI GRCGYC 1 cut(s) 174
AdeI CACNNNGTG 3 cut(s) 398, 400, 1343
AfaI GTAC 9 cut(s) 14, 316, 1096, 1242, 1324, 1521, 1794, 2098, 2552
AfeI AGCGCT 1 cut(s) 2029
AhlI ACTAGT 1 cut(s) 317
AjnI CCWGG 7 cut(s) 553, 838, 1126, 1393, 1501, 1747, 2197
AjuI GAANNNNNNNTTGG 2 cut(s) 2589, 2621
Alw21I GWGCWC 3 cut(s) 219, 1075, 2120
Alw26I GTCTC 9 cut(s) 47, 567, 837, 1966, 2085, 2235, 2570, 2631, 2738
AlwI GGATC 3 cut(s) 998, 1678, 2545
AlwNI CAGNNNCTG 2 cut(s) 2239, 2548
Ama87I CYCGRG 2 cut(s) 615, 1419
Aor13HI TCCGGA 1 cut(s) 742
Aor51HI AGCGCT 1 cut(s) 2029
AoxI GGCC 9 cut(s) 444, 1018, 1090, 1151, 1178, 1526, 2062, 2203, 2428
ApoI RAATTY 7 cut(s) 481, 730, 859, 1023, 1261, 1651, 2662
Asp700I GAANNNNTTC 3 cut(s) 930, 1038, 1885
Asp718I GGTACC 1 cut(s) 1322
AspA2I CCTAGG 1 cut(s) 2494
AspLEI GCGC 4 cut(s) 473, 978, 1925, 2030
AsuC2I CCSGG 1 cut(s) 2634
AsuHPI GGTGA 4 cut(s) 446, 868, 2021, 2801
AvaI CYCGRG 2 cut(s) 615, 1419
AvaII GGWCC 7 cut(s) 32, 411, 775, 1268, 1391, 2195, 2236
AvrII CCTAGG 1 cut(s) 2494
BaeGI GKGCMC 2 cut(s) 1641, 2494
BalI TGGCCA 2 cut(s) 2064, 2205
BanI GGYRCC 1 cut(s) 1322
BanII GRGCYC 1 cut(s) 367
BarI GAAGNNNNNNTAC 2 cut(s) 1468, 1500
BauI CACGAG 2 cut(s) 369, 2487
BbsI GAAGAC 2 cut(s) 2499, 2785
Bbv12I GWGCWC 3 cut(s) 219, 1075, 2120
BccI CCATC 5 cut(s) 298, 1468, 1562, 2006, 2165
BceAI ACGGC 3 cut(s) 1077, 1541, 1846
BciT130I CCWGG 7 cut(s) 555, 840, 1128, 1395, 1503, 1749, 2199
BclI TGATCA 2 cut(s) 163, 226
BcnI CCSGG 1 cut(s) 2634
BcoDI GTCTC 9 cut(s) 47, 567, 837, 1966, 2085, 2235, 2570, 2631, 2738
BcuI ACTAGT 1 cut(s) 317
BfaI CTAG 6 cut(s) 134, 318, 1067, 1347, 2025, 2495
BfmI CTRYAG 2 cut(s) 2240, 2652
BfoI RGCGCY 2 cut(s) 979, 2031
BfuAI ACCTGC 1 cut(s) 2152
BglI GCCNNNNNGGC 1 cut(s) 2498
BglII AGATCT 1 cut(s) 2754
BlnI CCTAGG 1 cut(s) 2494
BlpI GCTNAGC 2 cut(s) 327, 2517
BmcAI AGTACT 2 cut(s) 14, 2098
Bme1390I CCNGG 8 cut(s) 555, 840, 1128, 1395, 1503, 1749, 2199, 2634
Bme18I GGWCC 7 cut(s) 32, 411, 775, 1268, 1391, 2195, 2236
BmeT110I CYCGRG 2 cut(s) 615, 1419
BmiI GGNNCC 7 cut(s) 34, 366, 412, 447, 1179, 1324, 1753
BmrFI CCNGG 8 cut(s) 555, 840, 1128, 1395, 1503, 1749, 2199, 2634
BmrI ACTGGG 2 cut(s) 173, 2298
BmsI GCATC 8 cut(s) 719, 1004, 1359, 1502, 1603, 1808, 2296, 2463
BmuI ACTGGG 2 cut(s) 173, 2298
BoxI GACNNNNGTC 4 cut(s) 456, 1396, 2730, 2772
BpiI GAAGAC 2 cut(s) 2499, 2785
BplI GAGNNNNNCTC 2 cut(s) 2720, 2752
BpmI CTGGAG 4 cut(s) 726, 1149, 1683, 2722
Bpu10I CCTNAGC 1 cut(s) 876
Bpu1102I GCTNAGC 2 cut(s) 327, 2517
BpuEI CTTGAG 6 cut(s) 203, 552, 1103, 1587, 1822, 2804
BpuMI CCSGG 1 cut(s) 2634
BsaAI YACGTR 1 cut(s) 1676
BsaBI GATNNNNATC 2 cut(s) 80, 228
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 2 cut(s) 837, 2738
BsaJI CCNNGG 9 cut(s) 454, 838, 900, 1292, 1394, 1523, 1917, 2431, 2494
BsaWI WCCGGW 2 cut(s) 311, 742
BsaXI ACNNNNNCTCC 2 cut(s) 1122, 1152
Bse1I ACTGG 7 cut(s) 179, 1430, 1477, 1488, 1701, 2293, 2739
Bse3DI GCAATG 6 cut(s) 46, 266, 925, 966, 1062, 1443
Bse8I GATNNNNATC 2 cut(s) 80, 228
BseAI TCCGGA 1 cut(s) 742
BseBI CCWGG 7 cut(s) 555, 840, 1128, 1395, 1503, 1749, 2199
BseDI CCNNGG 9 cut(s) 454, 838, 900, 1292, 1394, 1523, 1917, 2431, 2494
BseGI GGATG 7 cut(s) 229, 229, 308, 1374, 1463, 2463, 2716
BseJI GATNNNNATC 2 cut(s) 80, 228
BseMI GCAATG 6 cut(s) 46, 266, 925, 966, 1062, 1443
BseNI ACTGG 7 cut(s) 179, 1430, 1477, 1488, 1701, 2293, 2739
BseRI GAGGAG 7 cut(s) 65, 357, 555, 633, 1531, 2439, 2575
BseSI GKGCMC 2 cut(s) 1641, 2494
BseYI CCCAGC 4 cut(s) 35, 1087, 1148, 2414
BsgI GTGCAG 6 cut(s) 939, 1217, 1320, 1434, 1667, 2740
BshFI GGCC 9 cut(s) 446, 1020, 1092, 1153, 1180, 1528, 2064, 2205, 2430
BshNI GGYRCC 1 cut(s) 1322
BsiHKAI GWGCWC 3 cut(s) 219, 1075, 2120
BsiHKCI CYCGRG 2 cut(s) 615, 1419
BsiSI CCGG 3 cut(s) 312, 743, 2633
BslFI GGGAC 7 cut(s) 18, 161, 424, 1131, 2132, 2738, 2780
BsmAI GTCTC 9 cut(s) 47, 567, 837, 1966, 2085, 2235, 2570, 2631, 2738
BsmBI CGTCTC 1 cut(s) 2631
BsmFI GGGAC 7 cut(s) 18, 161, 424, 1131, 2132, 2738, 2780
BsmI GAATGC 3 cut(s) 1150, 2362, 2632
BsnI GGCC 9 cut(s) 446, 1020, 1092, 1153, 1180, 1528, 2064, 2205, 2430
Bso31I GGTCTC 2 cut(s) 837, 2738
BsoBI CYCGRG 2 cut(s) 615, 1419
Bsp1286I GDGCHC 6 cut(s) 219, 367, 1075, 1641, 2120, 2494
Bsp13I TCCGGA 1 cut(s) 742
Bsp1407I TGTACA 1 cut(s) 2550
Bsp1720I GCTNAGC 2 cut(s) 327, 2517
BspACI CCGC 7 cut(s) 193, 778, 1111, 1452, 2314, 2345, 2514
BspANI GGCC 9 cut(s) 446, 1020, 1092, 1153, 1180, 1528, 2064, 2205, 2430
BspEI TCCGGA 1 cut(s) 742
BspLI GGNNCC 7 cut(s) 34, 366, 412, 447, 1179, 1324, 1753
BspMAI CTGCAG 1 cut(s) 2244
BspMI ACCTGC 1 cut(s) 2152
BspPI GGATC 3 cut(s) 998, 1678, 2545
BspQI GCTCTTC 1 cut(s) 1080
BspT107I GGYRCC 1 cut(s) 1322
BspTNI GGTCTC 2 cut(s) 837, 2738
BsrBI CCGCTC 1 cut(s) 2516
BsrDI GCAATG 6 cut(s) 46, 266, 925, 966, 1062, 1443
BsrGI TGTACA 1 cut(s) 2550
BsrI ACTGG 7 cut(s) 179, 1430, 1477, 1488, 1701, 2293, 2739
BssECI CCNNGG 9 cut(s) 454, 838, 900, 1292, 1394, 1523, 1917, 2431, 2494
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 2 cut(s) 369, 2487
BssT1I CCWWGG 5 cut(s) 454, 900, 1292, 2431, 2494
Bst2BI CACGAG 2 cut(s) 369, 2487
Bst2UI CCWGG 7 cut(s) 555, 840, 1128, 1395, 1503, 1749, 2199
Bst4CI ACNGT 6 cut(s) 70, 110, 398, 636, 2146, 2550
Bst6I CTCTTC 2 cut(s) 1080, 2001
BstACI GRCGYC 1 cut(s) 174
BstAPI GCANNNNNTGC 2 cut(s) 268, 1343
BstAUI TGTACA 1 cut(s) 2550
BstBAI YACGTR 1 cut(s) 1676
BstC8I GCNNGC 4 cut(s) 789, 1053, 1155, 1982
BstDSI CCRYGG 1 cut(s) 1523
BstENI CCTNNNNNAGG 1 cut(s) 1506
BstF5I GGATG 7 cut(s) 229, 229, 308, 1374, 1463, 2463, 2716
BstH2I RGCGCY 2 cut(s) 979, 2031
BstHHI GCGC 4 cut(s) 473, 978, 1925, 2030
BstMAI GTCTC 9 cut(s) 47, 567, 837, 1966, 2085, 2235, 2570, 2631, 2738
BstMWI GCNNNNNNNGC 9 cut(s) 268, 707, 953, 1057, 1154, 1343, 1645, 2498, 2688
BstNI CCWGG 7 cut(s) 555, 840, 1128, 1395, 1503, 1749, 2199
BstNSI RCATGY 2 cut(s) 532, 1319
BstPAI GACNNNNGTC 4 cut(s) 456, 1396, 2730, 2772
BstSCI CCNGG 8 cut(s) 553, 838, 1126, 1393, 1501, 1747, 2197, 2632
BstSFI CTRYAG 2 cut(s) 2240, 2652
BstSLI GKGCMC 2 cut(s) 1641, 2494
BstV2I GAAGAC 2 cut(s) 2499, 2785
BstX2I RGATCY 2 cut(s) 2537, 2754
BstXI CCANNNNNNTGG 1 cut(s) 2438
BstYI RGATCY 2 cut(s) 2537, 2754
BsuRI GGCC 9 cut(s) 446, 1020, 1092, 1153, 1180, 1528, 2064, 2205, 2430
BtgI CCRYGG 1 cut(s) 1523
BtsCI GGATG 7 cut(s) 229, 229, 308, 1374, 1463, 2463, 2716
BtsI GCAGTG 2 cut(s) 1641, 2309
BtsIMutI CAGTG 7 cut(s) 403, 1437, 1495, 1641, 2309, 2546, 2746
BveI ACCTGC 1 cut(s) 2152
Cac8I GCNNGC 4 cut(s) 789, 1053, 1155, 1982
CaiI CAGNNNCTG 2 cut(s) 2239, 2548
CfoI GCGC 4 cut(s) 473, 978, 1925, 2030
CseI GACGC 3 cut(s) 163, 755, 1728
CsiI ACCWGGT 1 cut(s) 1747
Csp6I GTAC 9 cut(s) 13, 315, 1095, 1241, 1323, 1520, 1793, 2097, 2551
CviAII CATG 8 cut(s) 442, 529, 593, 781, 944, 1114, 1316, 2533
CviQI GTAC 9 cut(s) 13, 315, 1095, 1241, 1323, 1520, 1793, 2097, 2551
DraIII CACNNNGTG 3 cut(s) 398, 400, 1343
DrdI GACNNNNNNGTC 1 cut(s) 94
DseDI GACNNNNNNGTC 1 cut(s) 94
EaeI YGGCCR 3 cut(s) 1526, 2062, 2203
Eam1104I CTCTTC 2 cut(s) 1080, 2001
EarI CTCTTC 2 cut(s) 1080, 2001
Eco130I CCWWGG 5 cut(s) 454, 900, 1292, 2431, 2494
Eco24I GRGCYC 1 cut(s) 367
Eco31I GGTCTC 2 cut(s) 837, 2738
Eco47I GGWCC 7 cut(s) 32, 411, 775, 1268, 1391, 2195, 2236
Eco47III AGCGCT 1 cut(s) 2029
Eco57I CTGAAG 4 cut(s) 915, 1767, 1887, 1982
Eco88I CYCGRG 2 cut(s) 615, 1419
EcoNI CCTNNNNNAGG 1 cut(s) 1506
EcoO109I RGGNCCY 4 cut(s) 411, 1178, 2195, 2236
EcoRI GAATTC 2 cut(s) 481, 1651
EcoRII CCWGG 7 cut(s) 553, 838, 1126, 1393, 1501, 1747, 2197
EcoT14I CCWWGG 5 cut(s) 454, 900, 1292, 2431, 2494
EcoT38I GRGCYC 1 cut(s) 367
ErhI CCWWGG 5 cut(s) 454, 900, 1292, 2431, 2494
Esp3I CGTCTC 1 cut(s) 2631
FaeI CATG 8 cut(s) 445, 532, 596, 784, 947, 1117, 1319, 2536
FalI AAGNNNNNCTT 4 cut(s) 1035, 1067, 1210, 1242
FaqI GGGAC 7 cut(s) 18, 161, 424, 1131, 2132, 2738, 2780
FatI CATG 8 cut(s) 441, 528, 592, 780, 943, 1113, 1315, 2532
FauI CCCGC 1 cut(s) 2521
FbaI TGATCA 2 cut(s) 163, 226
FblI GTMKAC 1 cut(s) 536
FokI GGATG 7 cut(s) 216, 236, 295, 1381, 1450, 2470, 2723
FriOI GRGCYC 1 cut(s) 367
FspBI CTAG 6 cut(s) 134, 318, 1067, 1347, 2025, 2495
GlaI GCGC 4 cut(s) 472, 977, 1924, 2029
GsaI CCCAGC 4 cut(s) 39, 1091, 1152, 2418
GsuI CTGGAG 4 cut(s) 726, 1149, 1683, 2722
HaeII RGCGCY 2 cut(s) 979, 2031
HaeIII GGCC 9 cut(s) 446, 1020, 1092, 1153, 1180, 1528, 2064, 2205, 2430
HapII CCGG 3 cut(s) 312, 743, 2633
HgaI GACGC 3 cut(s) 163, 755, 1728
HhaI GCGC 4 cut(s) 473, 978, 1925, 2030
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 8 cut(s) 445, 532, 596, 784, 947, 1117, 1319, 2536
Hin6I GCGC 4 cut(s) 471, 976, 1923, 2028
HinP1I GCGC 4 cut(s) 471, 976, 1923, 2028
HincII GTYRAC 2 cut(s) 2365, 2386
HindII GTYRAC 2 cut(s) 2365, 2386
HindIII AAGCTT 1 cut(s) 669
HpaII CCGG 3 cut(s) 312, 743, 2633
HphI GGTGA 4 cut(s) 446, 868, 2021, 2801
Hpy166II GTNNAC 8 cut(s) 537, 1303, 1717, 1793, 1847, 2365, 2386, 2696
Hpy188III TCNNGA 6 cut(s) 85, 743, 1082, 1785, 1815, 2303
Hpy8I GTNNAC 8 cut(s) 537, 1303, 1717, 1793, 1847, 2365, 2386, 2696
Hpy99I CGWCG 2 cut(s) 1177, 2778
HpyAV CCTTC 8 cut(s) 17, 424, 431, 578, 732, 876, 1358, 1771
HpyCH4III ACNGT 6 cut(s) 70, 110, 398, 636, 2146, 2550
HpyCH4IV ACGT 5 cut(s) 1531, 1675, 1966, 2640, 2773
HpyF10VI GCNNNNNNNGC 9 cut(s) 268, 707, 953, 1057, 1154, 1343, 1645, 2498, 2688
HpySE526I ACGT 5 cut(s) 1531, 1675, 1966, 2640, 2773
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 8 cut(s) 445, 532, 596, 784, 947, 1117, 1319, 2536
HspAI GCGC 4 cut(s) 471, 976, 1923, 2028
Kpn2I TCCGGA 1 cut(s) 742
KpnI GGTACC 1 cut(s) 1326
Ksp22I TGATCA 2 cut(s) 163, 226
LguI GCTCTTC 1 cut(s) 1080
LmnI GCTCC 7 cut(s) 370, 707, 784, 992, 1226, 1294, 2281
LweI GCATC 8 cut(s) 719, 1004, 1359, 1502, 1603, 1808, 2296, 2463
MabI ACCWGGT 1 cut(s) 1747
MaeI CTAG 6 cut(s) 134, 318, 1067, 1347, 2025, 2495
MaeII ACGT 5 cut(s) 1531, 1675, 1966, 2640, 2773
MaeIII GTNAC 4 cut(s) 297, 1740, 2053, 2544
MbiI CCGCTC 1 cut(s) 2516
MflI RGATCY 2 cut(s) 2537, 2754
MhlI GDGCHC 6 cut(s) 219, 367, 1075, 1641, 2120, 2494
MlsI TGGCCA 2 cut(s) 2064, 2205
MluNI TGGCCA 2 cut(s) 2064, 2205
MlyI GAGTC 7 cut(s) 104, 607, 1532, 1987, 2254, 2350, 2572
MmeI TCCRAC 5 cut(s) 403, 1648, 1941, 2488, 2620
Mox20I TGGCCA 2 cut(s) 2064, 2205
MroI TCCGGA 1 cut(s) 742
MroXI GAANNNNTTC 3 cut(s) 930, 1038, 1885
MscI TGGCCA 2 cut(s) 2064, 2205
MseI TTAA 3 cut(s) 519, 1569, 2255
MslI CAYNNNNRTG 5 cut(s) 597, 1068, 1193, 1314, 1386
Msp20I TGGCCA 2 cut(s) 2064, 2205
MspA1I CMGCKG 2 cut(s) 193, 2623
MspI CCGG 3 cut(s) 312, 743, 2633
MspR9I CCNGG 8 cut(s) 555, 840, 1128, 1395, 1503, 1749, 2199, 2634
Mva1269I GAATGC 3 cut(s) 1150, 2362, 2632
MvaI CCWGG 7 cut(s) 555, 840, 1128, 1395, 1503, 1749, 2199
MwoI GCNNNNNNNGC 9 cut(s) 268, 707, 953, 1057, 1154, 1343, 1645, 2498, 2688
NciI CCSGG 1 cut(s) 2634
NlaIII CATG 8 cut(s) 445, 532, 596, 784, 947, 1117, 1319, 2536
NlaIV GGNNCC 7 cut(s) 34, 366, 412, 447, 1179, 1324, 1753
NmuCI GTSAC 3 cut(s) 297, 1740, 2544
NspI RCATGY 2 cut(s) 532, 1319
PaeR7I CTCGAG 2 cut(s) 615, 1419
PciSI GCTCTTC 1 cut(s) 1080
PctI GAATGC 3 cut(s) 1150, 2362, 2632
PdmI GAANNNNTTC 3 cut(s) 930, 1038, 1885
PfeI GAWTC 7 cut(s) 62, 1034, 1252, 1406, 1811, 2044, 2792
PflMI CCANNNNNTGG 2 cut(s) 455, 2602
PfoI TCCNGGA 1 cut(s) 1126
PleI GAGTC 7 cut(s) 103, 607, 1532, 1987, 2253, 2349, 2571
PpsI GAGTC 7 cut(s) 103, 607, 1532, 1987, 2253, 2349, 2571
Ppu21I YACGTR 1 cut(s) 1676
PpuMI RGGWCCY 3 cut(s) 411, 2195, 2236
PshAI GACNNNNGTC 4 cut(s) 456, 1396, 2730, 2772
Psp5II RGGWCCY 3 cut(s) 411, 2195, 2236
Psp6I CCWGG 7 cut(s) 553, 838, 1126, 1393, 1501, 1747, 2197
PspFI CCCAGC 4 cut(s) 35, 1087, 1148, 2414
PspGI CCWGG 7 cut(s) 553, 838, 1126, 1393, 1501, 1747, 2197
PspN4I GGNNCC 7 cut(s) 34, 366, 412, 447, 1179, 1324, 1753
PspPPI RGGWCCY 3 cut(s) 411, 2195, 2236
PspXI VCTCGAGB 2 cut(s) 615, 1419
PstI CTGCAG 1 cut(s) 2244
PstNI CAGNNNCTG 2 cut(s) 2239, 2548
PsuI RGATCY 2 cut(s) 2537, 2754
PvuII CAGCTG 1 cut(s) 2623
RsaI GTAC 9 cut(s) 14, 316, 1096, 1242, 1324, 1521, 1794, 2098, 2552
RsaNI GTAC 9 cut(s) 13, 315, 1095, 1241, 1323, 1520, 1793, 2097, 2551
RseI CAYNNNNRTG 5 cut(s) 597, 1068, 1193, 1314, 1386
SapI GCTCTTC 1 cut(s) 1080
SaqAI TTAA 3 cut(s) 519, 1569, 2255
ScaI AGTACT 2 cut(s) 14, 2098
SchI GAGTC 7 cut(s) 104, 607, 1532, 1987, 2254, 2350, 2572
ScrFI CCNGG 8 cut(s) 555, 840, 1128, 1395, 1503, 1749, 2199, 2634
SduI GDGCHC 6 cut(s) 219, 367, 1075, 1641, 2120, 2494
SexAI ACCWGGT 1 cut(s) 1747
SfaNI GCATC 8 cut(s) 719, 1004, 1359, 1502, 1603, 1808, 2296, 2463
SfcI CTRYAG 2 cut(s) 2240, 2652
Sfr274I CTCGAG 2 cut(s) 615, 1419
SinI GGWCC 7 cut(s) 32, 411, 775, 1268, 1391, 2195, 2236
SlaI CTCGAG 2 cut(s) 615, 1419
SmiMI CAYNNNNRTG 5 cut(s) 597, 1068, 1193, 1314, 1386
SmlI CTYRAG 8 cut(s) 218, 567, 615, 1082, 1419, 1602, 1801, 2783
SmoI CTYRAG 8 cut(s) 218, 567, 615, 1082, 1419, 1602, 1801, 2783
SpeI ACTAGT 1 cut(s) 317
SsiI CCGC 7 cut(s) 193, 778, 1111, 1452, 2314, 2345, 2514
SspMI CTAG 6 cut(s) 134, 318, 1067, 1347, 2025, 2495
StyD4I CCNGG 8 cut(s) 553, 838, 1126, 1393, 1501, 1747, 2197, 2632
StyI CCWWGG 5 cut(s) 454, 900, 1292, 2431, 2494
TaaI ACNGT 6 cut(s) 70, 110, 398, 636, 2146, 2550
TaiI ACGT 5 cut(s) 1534, 1678, 1969, 2643, 2776
TaqI TCGA 5 cut(s) 616, 1250, 1420, 2453, 2776
TaqII GACCGA 1 cut(s) 1931
TatI WGTACW 3 cut(s) 12, 2096, 2550
TfiI GAWTC 7 cut(s) 62, 1034, 1252, 1406, 1811, 2044, 2792
Tru1I TTAA 3 cut(s) 519, 1569, 2255
Tru9I TTAA 3 cut(s) 519, 1569, 2255
TscAI CASTG 7 cut(s) 403, 1437, 1495, 1641, 2316, 2553, 2746
TseFI GTSAC 3 cut(s) 297, 1740, 2544
Tsp45I GTSAC 3 cut(s) 297, 1740, 2544
TspDTI ATGAA 6 cut(s) 36, 297, 1274, 1298, 1644, 2369
TspGWI ACGGA 2 cut(s) 1734, 2776
TspRI CASTG 7 cut(s) 403, 1437, 1495, 1641, 2316, 2553, 2746
Van91I CCANNNNNTGG 2 cut(s) 455, 2602
VpaK11BI GGWCC 7 cut(s) 32, 411, 775, 1268, 1391, 2195, 2236
XagI CCTNNNNNAGG 1 cut(s) 1506
XapI RAATTY 7 cut(s) 481, 730, 859, 1023, 1261, 1651, 2662
XceI RCATGY 2 cut(s) 532, 1319
XhoI CTCGAG 2 cut(s) 615, 1419
XmaJI CCTAGG 1 cut(s) 2494
XmiI GTMKAC 1 cut(s) 536
XmnI GAANNNNTTC 3 cut(s) 930, 1038, 1885
XspI CTAG 6 cut(s) 134, 318, 1067, 1347, 2025, 2495
ZrmI AGTACT 2 cut(s) 14, 2098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.