AT5G43290
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015149
Physical Location & Seq
Reverse (-)
17371808 .. 17373221
1414 bp
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UTR
Exon/CDS
Intron
AT5G43290.1

Sequence Viewer

Length: 825 bp
ATGGAAGAAGAAGGTTATCAGTGGGCAAGAAGGTGTGGAAACAACGCCGTTGAAGATCCTTTTGTTTATGAGCCACCTCTTTTCTTTCTGCCTCAAGACCAACATCATATGCATGGGCTCATGCCAAATGAAGATTTCATTGCCAACAAGTTCGTCACATCAACACTTTACTCCGGTCCACGAATCCAAGATATTGCAAACGCTCTGGCCTTGGTCGAACCCCTGACCCACCCAGTCCGAGAAATCTCTAAATCAACAGTTCCTCTTTTGGAAAGAAGTACTTTGAGCAAGGTGGATAGGTACACTTTAAAGGTGAAGAACAATAGTAATGGAATGTGTGATGATGGATACAAATGGAGAAAATATGGCCAAAAATCAATCAAAAATAGCCCTAACCCAAGGAGTTATTACAAGTGCACAAACCCAATATGCAATGCAAAGAAGCAAGTGGAGAGATCAATTGATGAGTCTAACACATATATCATTACCTACGAAGGTTTCCACTTCCACTATACTTACCCTTTCTTCCTACCCGATAAGACACGTCAATGGCCCAATAAAAAAACGAAAATACATAAACATAATGCTCAAGATATGAACAAAAAATCTCAAACCCAAGAAGAGAGCAAAGAAGCACAATTAGGTGAGCTCACCAACCAAAATCATCCAGTCAATAAAGCCCAAGAAAACACACCGGCAAATCTCGAAGAGGGATTGTTTTTTCCGGTTGATCAGTGTCGGCCGCAACAAGGGCTTCTAGAAGATGTGGTAGCTCCGGCAATGAAAAATATTCCTACCAGGGACAGTGTTTTGACAGCTTCTTGA

Protein Analysis

274

Amino Acids

31.58

Weight (kDa)

7.66

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 114 - 170 4.1e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43290
fragaria_vesca FvH4_6g13470
malus_domestica MD04G1131000.v1.1 MD12G1144100.v1.1
prunus_persica Prupe.6G257500_v2.0.a1
pyrus_communis pycom04g11930 pycom12g13870
rosa_chinensis RchiOBHm_Chr3g0466341
rosa_laevigata RLG00000024584
rosa_multiflora Rmu_sc0003480.1_g000013
rosa_roxburghii Rroxscaffold_6G00414560
rosa_rugosa Rorug03G0081100
rosa_samantha Rh3AG127400 Rh3BG148800 Rh3DG149500
rosa_wichuraiana Rw3G012040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 743
AclWI GGATC 1 cut(s) 50
AcoI YGGCCR 2 cut(s) 367, 740
AfaI GTAC 2 cut(s) 280, 302
AfiI CCNNNNNNNGG 1 cut(s) 749
AflIII ACRYGT 1 cut(s) 542
AgsI TTSAA 1 cut(s) 53
AjiI CACGTC 1 cut(s) 545
AjnI CCWGG 1 cut(s) 797
AluBI AGCT 3 cut(s) 649, 773, 818
AluI AGCT 3 cut(s) 649, 773, 818
Alw21I GWGCWC 2 cut(s) 419, 651
Alw44I GTGCAC 1 cut(s) 415
AlwI GGATC 1 cut(s) 50
AoxI GGCC 4 cut(s) 207, 367, 551, 740
ApaLI GTGCAC 1 cut(s) 415
AspS9I GGNCC 2 cut(s) 176, 552
AsuHPI GGTGA 3 cut(s) 325, 643, 656
AvaII GGWCC 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 419
BalI TGGCCA 1 cut(s) 369
BanII GRGCYC 2 cut(s) 120, 651
Bbv12I GWGCWC 2 cut(s) 419, 651
BccI CCATC 1 cut(s) 338
BceAI ACGGC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 799
BciVI GTATCC 1 cut(s) 341
BclI TGATCA 1 cut(s) 730
BfaI CTAG 1 cut(s) 758
BfuI GTATCC 1 cut(s) 341
BisI GCNGC 1 cut(s) 743
BlsI GCNGC 1 cut(s) 744
BmcAI AGTACT 1 cut(s) 280
Bme1390I CCNGG 1 cut(s) 799
Bme18I GGWCC 1 cut(s) 176
BmgBI CACGTC 1 cut(s) 545
BmgT120I GGNCC 2 cut(s) 176, 552
BmrFI CCNGG 1 cut(s) 799
BmrI ACTGGG 1 cut(s) 227
BmuI ACTGGG 1 cut(s) 227
BpuEI CTTGAG 2 cut(s) 78, 573
BsaJI CCNNGG 3 cut(s) 210, 398, 798
BsaWI WCCGGW 2 cut(s) 173, 724
Bsc4I CCNNNNNNNGG 1 cut(s) 749
Bse118I RCCGGY 1 cut(s) 694
Bse1I ACTGG 2 cut(s) 233, 668
Bse3DI GCAATG 3 cut(s) 138, 439, 786
BseBI CCWGG 1 cut(s) 799
BseDI CCNNGG 3 cut(s) 210, 398, 798
BseGI GGATG 1 cut(s) 664
BseLI CCNNNNNNNGG 1 cut(s) 749
BseMI GCAATG 3 cut(s) 138, 439, 786
BseNI ACTGG 2 cut(s) 233, 668
BseSI GKGCMC 1 cut(s) 419
BseX3I CGGCCG 1 cut(s) 740
Bsh1285I CGRYCG 1 cut(s) 743
BshFI GGCC 4 cut(s) 209, 369, 553, 742
BsiEI CGRYCG 1 cut(s) 743
BsiHKAI GWGCWC 2 cut(s) 419, 651
BsiSI CCGG 4 cut(s) 174, 695, 725, 776
BslFI GGGAC 1 cut(s) 815
BslI CCNNNNNNNGG 1 cut(s) 749
BsmFI GGGAC 1 cut(s) 815
BsnI GGCC 4 cut(s) 209, 369, 553, 742
Bsp1286I GDGCHC 3 cut(s) 120, 419, 651
Bsp143I GATC 3 cut(s) 55, 455, 730
BspACI CCGC 1 cut(s) 743
BspANI GGCC 4 cut(s) 209, 369, 553, 742
BspPI GGATC 1 cut(s) 50
BsrDI GCAATG 3 cut(s) 138, 439, 786
BsrFI RCCGGY 1 cut(s) 694
BsrI ACTGG 2 cut(s) 233, 668
BssAI RCCGGY 1 cut(s) 694
BssECI CCNNGG 3 cut(s) 210, 398, 798
BssMI GATC 3 cut(s) 55, 455, 730
BssT1I CCWWGG 2 cut(s) 210, 398
Bst2UI CCWGG 1 cut(s) 799
Bst4CI ACNGT 2 cut(s) 259, 806
Bst6I CTCTTC 2 cut(s) 615, 702
BstF5I GGATG 1 cut(s) 664
BstKTI GATC 3 cut(s) 58, 458, 733
BstMBI GATC 3 cut(s) 55, 455, 730
BstMCI CGRYCG 1 cut(s) 743
BstMWI GCNNNNNNNGC 1 cut(s) 751
BstNI CCWGG 1 cut(s) 799
BstSCI CCNGG 1 cut(s) 797
BstSLI GKGCMC 1 cut(s) 419
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BstZI CGGCCG 1 cut(s) 740
BsuI GTATCC 1 cut(s) 341
BsuRI GGCC 4 cut(s) 209, 369, 553, 742
BtrI CACGTC 1 cut(s) 545
BtsCI GGATG 1 cut(s) 664
BtsIMutI CAGTG 3 cut(s) 26, 740, 811
Cfr10I RCCGGY 1 cut(s) 694
Cfr13I GGNCC 2 cut(s) 176, 552
Csp6I GTAC 2 cut(s) 279, 301
CviAII CATG 2 cut(s) 113, 121
CviQI GTAC 2 cut(s) 279, 301
DpnI GATC 3 cut(s) 57, 457, 732
DpnII GATC 3 cut(s) 55, 455, 730
DraI TTTAAA 1 cut(s) 309
EaeI YGGCCR 2 cut(s) 367, 740
EagI CGGCCG 1 cut(s) 740
Eam1104I CTCTTC 2 cut(s) 615, 702
EarI CTCTTC 2 cut(s) 615, 702
Ecl136II GAGCTC 1 cut(s) 649
EclXI CGGCCG 1 cut(s) 740
Eco130I CCWWGG 2 cut(s) 210, 398
Eco24I GRGCYC 2 cut(s) 120, 651
Eco47I GGWCC 1 cut(s) 176
Eco52I CGGCCG 1 cut(s) 740
Eco53kI GAGCTC 1 cut(s) 649
EcoICRI GAGCTC 1 cut(s) 649
EcoRII CCWGG 1 cut(s) 797
EcoT14I CCWWGG 2 cut(s) 210, 398
EcoT22I ATGCAT 1 cut(s) 114
EcoT38I GRGCYC 2 cut(s) 120, 651
ErhI CCWWGG 2 cut(s) 210, 398
FaeI CATG 2 cut(s) 116, 124
FalI AAGNNNNNCTT 2 cut(s) 265, 297
FaqI GGGAC 1 cut(s) 815
FatI CATG 2 cut(s) 112, 120
FauNDI CATATG 1 cut(s) 108
FbaI TGATCA 1 cut(s) 730
Fnu4HI GCNGC 1 cut(s) 743
FokI GGATG 1 cut(s) 651
FriOI GRGCYC 2 cut(s) 120, 651
Fsp4HI GCNGC 1 cut(s) 743
FspBI CTAG 1 cut(s) 758
GluI GCNGC 1 cut(s) 743
HaeIII GGCC 4 cut(s) 209, 369, 553, 742
HapII CCGG 4 cut(s) 174, 695, 725, 776
Hin1II CATG 2 cut(s) 116, 124
HinfI GANTC 2 cut(s) 183, 467
HpaII CCGG 4 cut(s) 174, 695, 725, 776
HphI GGTGA 3 cut(s) 325, 643, 656
Hpy166II GTNNAC 3 cut(s) 179, 303, 417
Hpy188I TCNGA 1 cut(s) 239
Hpy188III TCNNGA 5 cut(s) 95, 590, 704, 758, 822
Hpy8I GTNNAC 3 cut(s) 179, 303, 417
HpyAV CCTTC 3 cut(s) 5, 24, 488
HpyCH4III ACNGT 2 cut(s) 259, 806
HpyCH4IV ACGT 1 cut(s) 544
HpyCH4V TGCA 5 cut(s) 112, 197, 417, 432, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 751
HpySE526I ACGT 1 cut(s) 544
Hsp92II CATG 2 cut(s) 116, 124
Ksp22I TGATCA 1 cut(s) 730
Kzo9I GATC 3 cut(s) 55, 455, 730
LmnI GCTCC 1 cut(s) 778
MaeI CTAG 1 cut(s) 758
MaeII ACGT 1 cut(s) 544
MaeIII GTNAC 1 cut(s) 154
MalI GATC 3 cut(s) 57, 457, 732
MboI GATC 3 cut(s) 55, 455, 730
MboII GAAGA 9 cut(s) 17, 20, 65, 143, 328, 517, 632, 719, 773
MfeI CAATTG 1 cut(s) 459
MflI RGATCY 1 cut(s) 55
MhlI GDGCHC 3 cut(s) 120, 419, 651
MlsI TGGCCA 1 cut(s) 369
MluCI AATT 2 cut(s) 459, 638
MluNI TGGCCA 1 cut(s) 369
MlyI GAGTC 1 cut(s) 476
MnlI CCTC 4 cut(s) 87, 102, 273, 703
Mox20I TGGCCA 1 cut(s) 369
Mph1103I ATGCAT 1 cut(s) 114
MscI TGGCCA 1 cut(s) 369
MseI TTAA 1 cut(s) 308
MslI CAYNNNNRTG 2 cut(s) 111, 547
Msp20I TGGCCA 1 cut(s) 369
MspI CCGG 4 cut(s) 174, 695, 725, 776
MspR9I CCNGG 1 cut(s) 799
MunI CAATTG 1 cut(s) 459
MvaI CCWGG 1 cut(s) 799
MwoI GCNNNNNNNGC 1 cut(s) 751
NdeI CATATG 1 cut(s) 108
NdeII GATC 3 cut(s) 55, 455, 730
NlaIII CATG 2 cut(s) 116, 124
NmuCI GTSAC 1 cut(s) 154
NsiI ATGCAT 1 cut(s) 114
PfeI GAWTC 1 cut(s) 183
PkrI GCNGC 1 cut(s) 744
PleI GAGTC 1 cut(s) 475
PpsI GAGTC 1 cut(s) 475
Psp124BI GAGCTC 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 797
PspGI CCWGG 1 cut(s) 797
PspPI GGNCC 2 cut(s) 176, 552
PsuI RGATCY 1 cut(s) 55
RsaI GTAC 2 cut(s) 280, 302
RsaNI GTAC 2 cut(s) 279, 301
RseI CAYNNNNRTG 2 cut(s) 111, 547
SacI GAGCTC 1 cut(s) 651
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 1 cut(s) 743
Sau3AI GATC 3 cut(s) 55, 455, 730
Sau96I GGNCC 2 cut(s) 176, 552
ScaI AGTACT 1 cut(s) 280
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 1 cut(s) 799
SduI GDGCHC 3 cut(s) 120, 419, 651
SinI GGWCC 1 cut(s) 176
SmiMI CAYNNNNRTG 2 cut(s) 111, 547
SmlI CTYRAG 2 cut(s) 93, 588
SmoI CTYRAG 2 cut(s) 93, 588
Sse9I AATT 2 cut(s) 459, 638
SsiI CCGC 1 cut(s) 743
SspI AATATT 1 cut(s) 790
SspMI CTAG 1 cut(s) 758
SstI GAGCTC 1 cut(s) 651
StyD4I CCNGG 1 cut(s) 797
StyI CCWWGG 2 cut(s) 210, 398
TaaI ACNGT 2 cut(s) 259, 806
TaiI ACGT 1 cut(s) 547
TaqI TCGA 2 cut(s) 216, 705
TasI AATT 2 cut(s) 459, 638
TatI WGTACW 1 cut(s) 278
TauI GCSGC 1 cut(s) 745
TfiI GAWTC 1 cut(s) 183
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 3 cut(s) 26, 740, 811
TseFI GTSAC 1 cut(s) 154
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 4 cut(s) 127, 144, 611, 797
TspRI CASTG 3 cut(s) 26, 740, 811
VneI GTGCAC 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 176
XbaI TCTAGA 1 cut(s) 757
XspI CTAG 1 cut(s) 758
ZrmI AGTACT 1 cut(s) 280
Zsp2I ATGCAT 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.