Rh3DG149500
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0015149
Physical Location & Seq
Forward (+)
12796998 .. 12798751
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG149500.1

Sequence Viewer

Length: 882 bp
ATGGAGGAAGCAATGAGTACTAATTGGCCGGATGGGTTGGAAGAGGAGCTTGTGAGGGAGCTTTTGGACAATGAGTCTCCGTTCTTTGTGCTACCAGAGGAGGCTGTAGAGTATCCGCAAATGAGTTTTTCGAACAAAGAGGGACTTAACCGGTTCGTTCAGAACGTCTATTCTGGACCAACAATCCATGATATTGAGACTGCTTTGTCTGTCACAACTGGGATAACAGTCCAACCCCAAGAACTCTCACAAGCCAGACTTTCAATATTGGAGAGAGGTTTGAGTAAGGTTGAGCACAAGTATACTCTGAAGATCAAAAGTTGTGACAGTGGAATGGCTGATGATGGATATAAATGGAGGAAGTATGGACAGAAATCCATCAAGAATAGCCCAAATCCTAGGAGCTACTACAGGTGCACAAACCCAAGATGCAGTGCAAAGAAGCAAGTGGAGAGATCTAGTGAGGATCCGGACACACTCATCATCACCTATGAAGGCCTCCACTTACACTTTGCATATCCATTTTTCCACCTTACCCAGGCCCAGAATCCGAATCCACCAACGAAAAAGCCCAAGAAGAGAACCTTGGCGGCACAACCGGAGGACCATGAGCAAGAAGCTCAAGAAAGCCCGCGGAGCGTTACTCCAGACCCGGAACATGATCTGCAGCCCGGCGCATTTACGGACCAACCTCAAGAGTTTGTGGAAGAAGAGAGGGGCTCACAAGGGTTGCTTGAAGATGTGGTGCCATTTATGATTAGGAAGCCTTCAAGTGCTTCTTCAAATTCTTCATGTTGCTCTTACCGTTCACCTCCAACCTCTCCTCCTTCTCTGTCATGGCCTAGTTACTTTGATGTTGGGATCAATCATAGCATTGGATGA

Protein Analysis

293

Amino Acids

33.08

Weight (kDa)

5.2

Isoelectric Point (pI)

59.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 114 - 170 6.8e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43290
fragaria_vesca FvH4_6g13470
malus_domestica MD04G1131000.v1.1 MD12G1144100.v1.1
prunus_persica Prupe.6G257500_v2.0.a1
pyrus_communis pycom04g11930 pycom12g13870
rosa_chinensis RchiOBHm_Chr3g0466341
rosa_laevigata RLG00000024584
rosa_multiflora Rmu_sc0003480.1_g000013
rosa_roxburghii Rroxscaffold_6G00414560
rosa_rugosa Rorug03G0081100
rosa_samantha Rh3AG127400 Rh3BG148800 Rh3DG149500
rosa_wichuraiana Rw3G012040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 745
AccI GTMKAC 1 cut(s) 302
AccII CGCG 1 cut(s) 634
AccIII TCCGGA 1 cut(s) 469
AciI CCGC 4 cut(s) 116, 590, 632, 634
AclWI GGATC 3 cut(s) 461, 474, 869
AcoI YGGCCR 1 cut(s) 26
AcsI RAATTY 1 cut(s) 784
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 1 cut(s) 19
AfiI CCNNNNNNNGG 1 cut(s) 538
AgeI ACCGGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 264, 737, 771, 783
AhdI GACNNNNNGTC 1 cut(s) 73
AjnI CCWGG 1 cut(s) 537
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AluBI AGCT 4 cut(s) 49, 61, 405, 620
AluI AGCT 4 cut(s) 49, 61, 405, 620
Alw21I GWGCWC 2 cut(s) 297, 419
Alw26I GTCTC 2 cut(s) 81, 191
Alw44I GTGCAC 1 cut(s) 415
AlwI GGATC 3 cut(s) 461, 474, 869
Aor13HI TCCGGA 1 cut(s) 469
AoxI GGCC 4 cut(s) 26, 496, 540, 839
ApaLI GTGCAC 1 cut(s) 415
ApeKI GCWGC 1 cut(s) 667
ApoI RAATTY 1 cut(s) 784
AsiGI ACCGGT 1 cut(s) 150
AspA2I CCTAGG 1 cut(s) 398
AspLEI GCGC 1 cut(s) 677
AspS9I GGNCC 4 cut(s) 176, 541, 604, 685
AsuC2I CCSGG 2 cut(s) 653, 672
AsuHPI GGTGA 2 cut(s) 478, 801
AsuII TTCGAA 1 cut(s) 131
AvaII GGWCC 3 cut(s) 176, 604, 685
AvrII CCTAGG 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 419
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 745
BanII GRGCYC 1 cut(s) 722
Bbv12I GWGCWC 2 cut(s) 297, 419
BbvI GCAGC 1 cut(s) 679
BccI CCATC 3 cut(s) 26, 338, 386
BciT130I CCWGG 1 cut(s) 539
BciVI GTATCC 1 cut(s) 123
BcnI CCSGG 2 cut(s) 653, 672
BcoDI GTCTC 2 cut(s) 81, 191
BfaI CTAG 3 cut(s) 399, 459, 843
BfmI CTRYAG 3 cut(s) 105, 409, 665
BfuI GTATCC 1 cut(s) 123
BglII AGATCT 1 cut(s) 455
BisI GCNGC 2 cut(s) 591, 668
BlnI CCTAGG 1 cut(s) 398
BlsI GCNGC 2 cut(s) 592, 669
BmcAI AGTACT 1 cut(s) 19
Bme1390I CCNGG 3 cut(s) 539, 653, 672
Bme18I GGWCC 3 cut(s) 176, 604, 685
BmeRI GACNNNNNGTC 1 cut(s) 73
BmgT120I GGNCC 4 cut(s) 176, 541, 604, 685
BmiI GGNNCC 2 cut(s) 468, 747
BmrFI CCNGG 3 cut(s) 539, 653, 672
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 419
BmuI ACTGGG 1 cut(s) 228
BplI GAGNNNNNCTC 4 cut(s) 628, 660, 704, 736
BpmI CTGGAG 1 cut(s) 630
Bpu14I TTCGAA 1 cut(s) 131
BpuEI CTTGAG 2 cut(s) 606, 678
BpuMI CCSGG 2 cut(s) 653, 672
BsaJI CCNNGG 4 cut(s) 398, 537, 585, 632
BsaWI WCCGGW 3 cut(s) 150, 469, 598
BsaXI ACNNNNNCTCC 2 cut(s) 808, 838
Bsc4I CCNNNNNNNGG 1 cut(s) 538
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 1 cut(s) 223
Bse3DI GCAATG 1 cut(s) 18
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 1 cut(s) 539
BseDI CCNNGG 4 cut(s) 398, 537, 585, 632
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 1 cut(s) 538
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 1 cut(s) 223
BseRI GAGGAG 3 cut(s) 59, 113, 813
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 1 cut(s) 679
Bsh1236I CGCG 1 cut(s) 634
BshFI GGCC 4 cut(s) 28, 498, 542, 841
BshNI GGYRCC 1 cut(s) 745
BshTI ACCGGT 1 cut(s) 150
BsiHKAI GWGCWC 2 cut(s) 297, 419
BsiSI CCGG 6 cut(s) 29, 151, 470, 599, 653, 672
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 538
BsmAI GTCTC 2 cut(s) 81, 191
BsmFI GGGAC 1 cut(s) 156
BsnI GGCC 4 cut(s) 28, 498, 542, 841
Bsp119I TTCGAA 1 cut(s) 131
Bsp1286I GDGCHC 3 cut(s) 297, 419, 722
Bsp13I TCCGGA 1 cut(s) 469
Bsp143I GATC 5 cut(s) 312, 455, 466, 661, 861
BspACI CCGC 4 cut(s) 116, 590, 632, 634
BspANI GGCC 4 cut(s) 28, 498, 542, 841
BspEI TCCGGA 1 cut(s) 469
BspFNI CGCG 1 cut(s) 634
BspLI GGNNCC 2 cut(s) 468, 747
BspMAI CTGCAG 1 cut(s) 669
BspPI GGATC 3 cut(s) 461, 474, 869
BspT104I TTCGAA 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 745
BsrDI GCAATG 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 150
BsrI ACTGG 1 cut(s) 223
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 4 cut(s) 398, 537, 585, 632
BssMI GATC 5 cut(s) 312, 455, 466, 661, 861
BssNAI GTATAC 1 cut(s) 303
BssT1I CCWWGG 2 cut(s) 398, 585
Bst1107I GTATAC 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 3 cut(s) 229, 329, 806
Bst6I CTCTTC 3 cut(s) 36, 572, 705
BstBI TTCGAA 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 632
BstDSI CCRYGG 1 cut(s) 632
BstENI CCTNNNNNAGG 1 cut(s) 536
BstF5I GGATG 1 cut(s) 37
BstFNI CGCG 1 cut(s) 634
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 5 cut(s) 315, 458, 469, 664, 864
BstMAI GTCTC 2 cut(s) 81, 191
BstMBI GATC 5 cut(s) 312, 455, 466, 661, 861
BstMWI GCNNNNNNNGC 1 cut(s) 636
BstNI CCWGG 1 cut(s) 539
BstSCI CCNGG 3 cut(s) 537, 651, 670
BstSFI CTRYAG 3 cut(s) 105, 409, 665
BstSLI GKGCMC 1 cut(s) 419
BstUI CGCG 1 cut(s) 634
BstV1I GCAGC 1 cut(s) 679
BstX2I RGATCY 2 cut(s) 455, 466
BstYI RGATCY 2 cut(s) 455, 466
BstZ17I GTATAC 1 cut(s) 303
BsuI GTATCC 1 cut(s) 123
BsuRI GGCC 4 cut(s) 28, 498, 542, 841
BtgI CCRYGG 1 cut(s) 632
BtsCI GGATG 1 cut(s) 37
BtsI GCAGTG 1 cut(s) 439
BtsIMutI CAGTG 2 cut(s) 334, 439
Cac8I GCNNGC 1 cut(s) 632
CfoI GCGC 1 cut(s) 677
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 4 cut(s) 176, 541, 604, 685
Cfr42I CCGCGG 1 cut(s) 635
Csp6I GTAC 1 cut(s) 18
CspAI ACCGGT 1 cut(s) 150
CviAII CATG 5 cut(s) 188, 608, 659, 792, 837
CviQI GTAC 1 cut(s) 18
DpnI GATC 5 cut(s) 314, 457, 468, 663, 863
DpnII GATC 5 cut(s) 312, 455, 466, 661, 861
DrdI GACNNNNNNGTC 1 cut(s) 205
DriI GACNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 26
Eam1104I CTCTTC 3 cut(s) 36, 572, 705
Eam1105I GACNNNNNGTC 1 cut(s) 73
EarI CTCTTC 3 cut(s) 36, 572, 705
Eco130I CCWWGG 2 cut(s) 398, 585
Eco147I AGGCCT 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 722
Eco47I GGWCC 3 cut(s) 176, 604, 685
Eco57I CTGAAG 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 536
EcoRII CCWGG 1 cut(s) 537
EcoT14I CCWWGG 2 cut(s) 398, 585
EcoT38I GRGCYC 1 cut(s) 722
ErhI CCWWGG 2 cut(s) 398, 585
FaeI CATG 5 cut(s) 191, 611, 662, 795, 840
FaqI GGGAC 1 cut(s) 156
FatI CATG 5 cut(s) 187, 607, 658, 791, 836
FauI CCCGC 1 cut(s) 639
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 2 cut(s) 591, 668
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 1 cut(s) 722
Fsp4HI GCNGC 2 cut(s) 591, 668
FspBI CTAG 3 cut(s) 399, 459, 843
GlaI GCGC 1 cut(s) 676
GluI GCNGC 2 cut(s) 591, 668
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 4 cut(s) 28, 498, 542, 841
HapII CCGG 6 cut(s) 29, 151, 470, 599, 653, 672
HhaI GCGC 1 cut(s) 677
Hin1II CATG 5 cut(s) 191, 611, 662, 795, 840
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HinfI GANTC 3 cut(s) 74, 547, 553
HpaII CCGG 6 cut(s) 29, 151, 470, 599, 653, 672
HphI GGTGA 2 cut(s) 478, 801
Hpy166II GTNNAC 3 cut(s) 303, 417, 809
Hpy188I TCNGA 3 cut(s) 162, 309, 552
Hpy188III TCNNGA 6 cut(s) 174, 382, 470, 623, 647, 695
Hpy8I GTNNAC 3 cut(s) 303, 417, 809
HpyAV CCTTC 3 cut(s) 488, 777, 837
HpyCH4III ACNGT 3 cut(s) 229, 329, 806
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 5 cut(s) 417, 432, 437, 515, 667
HpyF10VI GCNNNNNNNGC 1 cut(s) 636
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 5 cut(s) 191, 611, 662, 795, 840
HspAI GCGC 1 cut(s) 675
Kpn2I TCCGGA 1 cut(s) 469
KspI CCGCGG 1 cut(s) 635
Kzo9I GATC 5 cut(s) 312, 455, 466, 661, 861
LmnI GCTCC 4 cut(s) 46, 58, 402, 636
Lsp1109I GCAGC 1 cut(s) 679
LweI GCATC 1 cut(s) 419
MaeI CTAG 3 cut(s) 399, 459, 843
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 4 cut(s) 211, 323, 640, 845
MalI GATC 5 cut(s) 314, 457, 468, 663, 863
MboI GATC 5 cut(s) 312, 455, 466, 661, 861
MboII GAAGA 8 cut(s) 53, 322, 589, 719, 722, 749, 771, 780
MflI RGATCY 2 cut(s) 455, 466
MhlI GDGCHC 3 cut(s) 297, 419, 722
MluCI AATT 2 cut(s) 22, 784
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 3 cut(s) 18, 256, 839
MroI TCCGGA 1 cut(s) 469
MseI TTAA 1 cut(s) 147
MspA1I CMGCKG 1 cut(s) 634
MspI CCGG 6 cut(s) 29, 151, 470, 599, 653, 672
MspR9I CCNGG 3 cut(s) 539, 653, 672
MvaI CCWGG 1 cut(s) 539
MvnI CGCG 1 cut(s) 634
MwoI GCNNNNNNNGC 1 cut(s) 636
NciI CCSGG 2 cut(s) 653, 672
NdeII GATC 5 cut(s) 312, 455, 466, 661, 861
NlaIII CATG 5 cut(s) 191, 611, 662, 795, 840
NlaIV GGNNCC 2 cut(s) 468, 747
NmuCI GTSAC 2 cut(s) 211, 323
NspV TTCGAA 1 cut(s) 131
PceI AGGCCT 1 cut(s) 498
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PfeI GAWTC 2 cut(s) 547, 553
PinAI ACCGGT 1 cut(s) 150
PkrI GCNGC 2 cut(s) 592, 669
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
Psp6I CCWGG 1 cut(s) 537
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 2 cut(s) 468, 747
PspPI GGNCC 4 cut(s) 176, 541, 604, 685
PstI CTGCAG 1 cut(s) 669
PsuI RGATCY 2 cut(s) 455, 466
RsaI GTAC 1 cut(s) 19
RsaNI GTAC 1 cut(s) 18
SacII CCGCGG 1 cut(s) 635
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 2 cut(s) 591, 668
Sau3AI GATC 5 cut(s) 312, 455, 466, 661, 861
Sau96I GGNCC 4 cut(s) 176, 541, 604, 685
ScaI AGTACT 1 cut(s) 19
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 3 cut(s) 539, 653, 672
SduI GDGCHC 3 cut(s) 297, 419, 722
SfaNI GCATC 1 cut(s) 419
SfcI CTRYAG 3 cut(s) 105, 409, 665
Sfr303I CCGCGG 1 cut(s) 635
SfuI TTCGAA 1 cut(s) 131
SgrBI CCGCGG 1 cut(s) 635
SinI GGWCC 3 cut(s) 176, 604, 685
SmlI CTYRAG 2 cut(s) 621, 693
SmoI CTYRAG 2 cut(s) 621, 693
Sse9I AATT 2 cut(s) 22, 784
SseBI AGGCCT 1 cut(s) 498
SsiI CCGC 4 cut(s) 116, 590, 632, 634
SspI AATATT 1 cut(s) 267
SspMI CTAG 3 cut(s) 399, 459, 843
StuI AGGCCT 1 cut(s) 498
StyD4I CCNGG 3 cut(s) 537, 651, 670
StyI CCWWGG 2 cut(s) 398, 585
TaaI ACNGT 3 cut(s) 229, 329, 806
TaiI ACGT 1 cut(s) 168
TaqI TCGA 1 cut(s) 131
TasI AATT 2 cut(s) 22, 784
TatI WGTACW 1 cut(s) 17
TauI GCSGC 1 cut(s) 593
TfiI GAWTC 2 cut(s) 547, 553
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 2 cut(s) 334, 439
TseFI GTSAC 2 cut(s) 211, 323
TseI GCWGC 1 cut(s) 667
Tsp45I GTSAC 2 cut(s) 211, 323
TspDTI ATGAA 2 cut(s) 507, 780
TspGWI ACGGA 2 cut(s) 69, 698
TspRI CASTG 2 cut(s) 334, 439
VneI GTGCAC 1 cut(s) 415
VpaK11BI GGWCC 3 cut(s) 176, 604, 685
XagI CCTNNNNNAGG 1 cut(s) 536
XapI RAATTY 1 cut(s) 784
XmaJI CCTAGG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 302
XspI CTAG 3 cut(s) 399, 459, 843
ZrmI AGTACT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.