MD12G1144100.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015149
Physical Location & Seq
Reverse (-)
22324583 .. 22326472
1890 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1144100.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGAGGAAGCAATGGGTGCTACGTGGTCGGACGGCTCAGAGGAAGAGCTGGTGAGGGAGCTTCTGGACAATGAGTCACCGTTCTTTGTGCTACCGGAGGAATCTGTGCAGTCCCGAACGACTAGTGCTTTCGGCAAAGAGGCTATCAACGGGTTTATCCCTAACGTCTATTCTGGTCCGACAATCCAAGATATTGAGACTGCTTTGTCTGTCACAACGGGAACAGTCCAGCCCCAAGAACTTTCAAATGCCAGGCTGTCGTTGTTAGAAAGGGGTCTGAGTAAGGTTGAGCATAAGTATATTCTGAAGATCAAAAGCTGTGGAAATGGAATAATGGCTGATGATGGTTATAAATGGAGGAAATATGGCCAGAAGTCCATTAAGAATAGCCCAAATCCTAGGAGCTACTACAGGTGCACAAATCCAAGATGCAGTGCCAAAAAGCAAGTAGAGAGGTCCAGTGAGGAACCAGACACGCTCATTATCACCTATGAAGGGCTCCATTTGCACTTCGCATATCCATTTTTTGACTTCAACAACCAGGCCCAAACCTCCAGCCCGTCAATGAAGAAGCCCAAGAAGAAAACCTCTGTGCCCCAAGCCGAAGACCACGACAGAGAAGCCCAAGAAAGTCTGAGAAGCGTTACTCCCGATCCAGTTCGGGATCTGCCACCCGGCCCGTTTCCGGGCCGGCATCAAGGGTATGTGGAAGAAGAAAGAAGCTCACAAGGATTGCTTCAAGATATGGTGCCATTCATGATTAGGAACCCTAAACCATCAAGTGCTTCTTCAAACTCTACATGTTCCTCTTCTCGTTCATCTCCAACCTCTCCTTCTTCTCTATCATGGGTTTCTTCCTATTTTGACGTGGGCATAAATCATAATTTTGGATGA

Protein Analysis

298

Amino Acids

33.08

Weight (kDa)

5.94

Isoelectric Point (pI)

59.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 114 - 170 1.6e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43290
fragaria_vesca FvH4_6g13470
malus_domestica MD04G1131000.v1.1 MD12G1144100.v1.1
prunus_persica Prupe.6G257500_v2.0.a1
pyrus_communis pycom04g11930 pycom12g13870
rosa_chinensis RchiOBHm_Chr3g0466341
rosa_laevigata RLG00000024584
rosa_multiflora Rmu_sc0003480.1_g000013
rosa_roxburghii Rroxscaffold_6G00414560
rosa_rugosa Rorug03G0081100
rosa_samantha Rh3AG127400 Rh3BG148800 Rh3DG149500
rosa_wichuraiana Rw3G012040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AasI GACNNNNNNGTC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 748
AclWI GGATC 2 cut(s) 647, 672
AcoI YGGCCR 1 cut(s) 367
AcuI CTGAAG 1 cut(s) 326
AfiI CCNNNNNNNGG 3 cut(s) 495, 685, 686
AflIII ACRYGT 1 cut(s) 800
AgsI TTSAA 4 cut(s) 246, 535, 740, 792
AhdI GACNNNNNGTC 1 cut(s) 73
AhlI ACTAGT 1 cut(s) 122
AjiI CACGTC 1 cut(s) 868
AjnI CCWGG 2 cut(s) 251, 540
AjuI GAANNNNNNNTTGG 2 cut(s) 817, 849
AluBI AGCT 5 cut(s) 49, 61, 318, 405, 723
AluI AGCT 5 cut(s) 49, 61, 318, 405, 723
Alw21I GWGCWC 1 cut(s) 419
Alw26I GTCTC 1 cut(s) 191
Alw44I GTGCAC 1 cut(s) 415
AlwI GGATC 2 cut(s) 647, 672
AoxI GGCC 4 cut(s) 367, 543, 676, 688
ApaLI GTGCAC 1 cut(s) 415
AspA2I CCTAGG 1 cut(s) 398
AspS9I GGNCC 5 cut(s) 176, 456, 544, 677, 688
AsuC2I CCSGG 2 cut(s) 675, 687
AsuHPI GGTGA 3 cut(s) 64, 69, 478
AvaII GGWCC 2 cut(s) 176, 456
AvrII CCTAGG 1 cut(s) 398
BaeGI GKGCMC 2 cut(s) 419, 597
BalI TGGCCA 1 cut(s) 369
BanI GGYRCC 1 cut(s) 748
BanII GRGCYC 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 419
BccI CCATC 2 cut(s) 338, 784
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 2 cut(s) 253, 542
BcnI CCSGG 2 cut(s) 675, 687
BcoDI GTCTC 1 cut(s) 191
BcuI ACTAGT 1 cut(s) 122
BfaI CTAG 2 cut(s) 123, 399
BfmI CTRYAG 1 cut(s) 409
BlnI CCTAGG 1 cut(s) 398
Bme1390I CCNGG 4 cut(s) 253, 542, 675, 687
Bme18I GGWCC 2 cut(s) 176, 456
BmeRI GACNNNNNGTC 1 cut(s) 73
BmgBI CACGTC 1 cut(s) 868
BmgT120I GGNCC 5 cut(s) 176, 456, 544, 677, 688
BmiI GGNNCC 4 cut(s) 468, 500, 750, 767
BmrFI CCNGG 4 cut(s) 253, 542, 675, 687
BmsI GCATC 2 cut(s) 419, 703
BpiI GAAGAC 1 cut(s) 612
BpmI CTGGAG 1 cut(s) 538
BpuMI CCSGG 2 cut(s) 675, 687
BsaAI YACGTR 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 398
BsaWI WCCGGW 1 cut(s) 94
Bsc4I CCNNNNNNNGG 3 cut(s) 495, 685, 686
Bse118I RCCGGY 1 cut(s) 690
Bse1I ACTGG 2 cut(s) 459, 656
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 2 cut(s) 253, 542
BseDI CCNNGG 1 cut(s) 398
BseLI CCNNNNNNNGG 3 cut(s) 495, 685, 686
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 3 cut(s) 51, 269, 626
BseNI ACTGG 2 cut(s) 459, 656
BseSI GKGCMC 2 cut(s) 419, 597
BsgI GTGCAG 1 cut(s) 128
BshFI GGCC 4 cut(s) 369, 545, 678, 690
BshNI GGYRCC 1 cut(s) 748
BsiHKAI GWGCWC 1 cut(s) 419
BsiSI CCGG 4 cut(s) 95, 675, 686, 691
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 3 cut(s) 495, 685, 686
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 4 cut(s) 369, 545, 678, 690
Bsp1286I GDGCHC 3 cut(s) 419, 501, 597
Bsp143I GATC 3 cut(s) 309, 652, 664
BspANI GGCC 4 cut(s) 369, 545, 678, 690
BspCNI CTCAG 3 cut(s) 50, 270, 627
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 4 cut(s) 468, 500, 750, 767
BspPI GGATC 2 cut(s) 647, 672
BspQI GCTCTTC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 748
BsrDI GCAATG 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 690
BsrI ACTGG 2 cut(s) 459, 656
BssAI RCCGGY 1 cut(s) 690
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 3 cut(s) 309, 652, 664
BssT1I CCWWGG 1 cut(s) 398
Bst2UI CCWGG 2 cut(s) 253, 542
Bst4CI ACNGT 2 cut(s) 81, 226
Bst6I CTCTTC 2 cut(s) 39, 814
BstAPI GCANNNNNTGC 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 24
BstC8I GCNNGC 1 cut(s) 692
BstDEI CTNAG 3 cut(s) 37, 278, 635
BstKTI GATC 3 cut(s) 312, 655, 667
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 3 cut(s) 309, 652, 664
BstMWI GCNNNNNNNGC 2 cut(s) 17, 505
BstNI CCWGG 2 cut(s) 253, 542
BstNSI RCATGY 1 cut(s) 804
BstSCI CCNGG 4 cut(s) 251, 540, 673, 685
BstSFI CTRYAG 1 cut(s) 409
BstSLI GKGCMC 2 cut(s) 419, 597
BstV2I GAAGAC 1 cut(s) 612
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BsuRI GGCC 4 cut(s) 369, 545, 678, 690
BtrI CACGTC 1 cut(s) 868
BtsI GCAGTG 1 cut(s) 439
BtsIMutI CAGTG 2 cut(s) 439, 466
Cac8I GCNNGC 1 cut(s) 692
CciI TCATGA 1 cut(s) 756
Cfr10I RCCGGY 1 cut(s) 690
Cfr13I GGNCC 5 cut(s) 176, 456, 544, 677, 688
CspCI CAANNNNNGTGG 2 cut(s) 301, 336
CviAII CATG 3 cut(s) 757, 801, 846
DdeI CTNAG 3 cut(s) 37, 278, 635
DpnI GATC 3 cut(s) 311, 654, 666
DpnII GATC 3 cut(s) 309, 652, 664
DrdI GACNNNNNNGTC 1 cut(s) 205
DriI GACNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 367
Eam1104I CTCTTC 2 cut(s) 39, 814
Eam1105I GACNNNNNGTC 1 cut(s) 73
EarI CTCTTC 2 cut(s) 39, 814
Eco130I CCWWGG 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 501
Eco47I GGWCC 2 cut(s) 176, 456
Eco57I CTGAAG 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 251, 540
EcoT14I CCWWGG 1 cut(s) 398
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 398
FaeI CATG 3 cut(s) 760, 804, 849
FalI AAGNNNNNCTT 4 cut(s) 720, 752, 772, 804
FaqI GGGAC 1 cut(s) 97
FatI CATG 3 cut(s) 756, 800, 845
FriOI GRGCYC 1 cut(s) 501
FspBI CTAG 2 cut(s) 123, 399
GsuI CTGGAG 1 cut(s) 538
HaeIII GGCC 4 cut(s) 369, 545, 678, 690
HapII CCGG 4 cut(s) 95, 675, 686, 691
Hin1II CATG 3 cut(s) 760, 804, 849
HinfI GANTC 2 cut(s) 74, 101
HpaII CCGG 4 cut(s) 95, 675, 686, 691
HphI GGTGA 3 cut(s) 64, 69, 478
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 6 cut(s) 31, 40, 180, 279, 306, 636
Hpy188III TCNNGA 6 cut(s) 65, 114, 650, 662, 740, 757
Hpy8I GTNNAC 1 cut(s) 417
HpyAV CCTTC 2 cut(s) 488, 843
HpyCH4III ACNGT 2 cut(s) 81, 226
HpyCH4IV ACGT 3 cut(s) 23, 165, 867
HpyCH4V TGCA 4 cut(s) 109, 417, 432, 508
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 505
HpyF3I CTNAG 3 cut(s) 37, 278, 635
HpySE526I ACGT 3 cut(s) 23, 165, 867
Hsp92II CATG 3 cut(s) 760, 804, 849
KroI GCCGGC 1 cut(s) 690
KroNI GCCGGC 1 cut(s) 692
Kzo9I GATC 3 cut(s) 309, 652, 664
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 3 cut(s) 58, 402, 504
LweI GCATC 2 cut(s) 419, 703
MaeI CTAG 2 cut(s) 123, 399
MaeII ACGT 3 cut(s) 23, 165, 867
MaeIII GTNAC 3 cut(s) 75, 211, 643
MalI GATC 3 cut(s) 311, 654, 666
MboI GATC 3 cut(s) 309, 652, 664
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 3 cut(s) 419, 501, 597
MlsI TGGCCA 1 cut(s) 369
MluCI AATT 1 cut(s) 883
MluNI TGGCCA 1 cut(s) 369
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 3 cut(s) 9, 203, 848
Mox20I TGGCCA 1 cut(s) 369
MroNI GCCGGC 1 cut(s) 690
MscI TGGCCA 1 cut(s) 369
MseI TTAA 1 cut(s) 381
Msp20I TGGCCA 1 cut(s) 369
MspI CCGG 4 cut(s) 95, 675, 686, 691
MspR9I CCNGG 4 cut(s) 253, 542, 675, 687
MvaI CCWGG 2 cut(s) 253, 542
MwoI GCNNNNNNNGC 2 cut(s) 17, 505
NaeI GCCGGC 1 cut(s) 692
NciI CCSGG 2 cut(s) 675, 687
NdeII GATC 3 cut(s) 309, 652, 664
NgoMIV GCCGGC 1 cut(s) 690
NlaIII CATG 3 cut(s) 760, 804, 849
NlaIV GGNNCC 4 cut(s) 468, 500, 750, 767
NmuCI GTSAC 2 cut(s) 75, 211
NspI RCATGY 1 cut(s) 804
PagI TCATGA 1 cut(s) 756
PciI ACATGT 1 cut(s) 800
PciSI GCTCTTC 1 cut(s) 39
PdiI GCCGGC 1 cut(s) 692
PfeI GAWTC 1 cut(s) 101
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
Ppu21I YACGTR 1 cut(s) 24
PscI ACATGT 1 cut(s) 800
PsiI TTATAA 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 251, 540
PspGI CCWGG 2 cut(s) 251, 540
PspN4I GGNNCC 4 cut(s) 468, 500, 750, 767
PspPI GGNCC 5 cut(s) 176, 456, 544, 677, 688
PsuI RGATCY 1 cut(s) 664
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 1 cut(s) 381
Sau3AI GATC 3 cut(s) 309, 652, 664
Sau96I GGNCC 5 cut(s) 176, 456, 544, 677, 688
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 4 cut(s) 253, 542, 675, 687
SduI GDGCHC 3 cut(s) 419, 501, 597
SfaNI GCATC 2 cut(s) 419, 703
SfcI CTRYAG 1 cut(s) 409
SinI GGWCC 2 cut(s) 176, 456
SpeI ACTAGT 1 cut(s) 122
Sse9I AATT 1 cut(s) 883
SspMI CTAG 2 cut(s) 123, 399
StyD4I CCNGG 4 cut(s) 251, 540, 673, 685
StyI CCWWGG 1 cut(s) 398
TaaI ACNGT 2 cut(s) 81, 226
TaiI ACGT 3 cut(s) 26, 168, 870
TasI AATT 1 cut(s) 883
TfiI GAWTC 1 cut(s) 101
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 2 cut(s) 439, 466
TseFI GTSAC 2 cut(s) 75, 211
Tsp45I GTSAC 2 cut(s) 75, 211
TspDTI ATGAA 4 cut(s) 507, 581, 745, 807
TspRI CASTG 2 cut(s) 439, 466
VneI GTGCAC 1 cut(s) 415
VpaK11BI GGWCC 2 cut(s) 176, 456
XceI RCATGY 1 cut(s) 804
XmaJI CCTAGG 1 cut(s) 398
XspI CTAG 2 cut(s) 123, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.