Rmu_sc0003480.1_g000013
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003480.1
OG0015149
Physical Location & Seq
Reverse (-)
55867 .. 57604
1738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003480.1_g000013.1.cds

Sequence Viewer

Length: 879 bp
atggaggaagcaatgagtactaattggccggatgggttggaagaggagcttgtgagggagcttttggacaatgagtctccgttctttgtgctaccagaggaggctgtagagtatccgcaaatgagtgtttcgaacaaagagggacttaaccggttcgtgcagaacgtctattctggaccaacaatccatgatattgagactgctttgtctgtcacaactgggataacagtccaaccccaagaactctcacaagccagactttcaatattggagaggggtttgagtaaggttgagcacaagtatactctgaagatcaaaagttgtgacagtggaatggctgatgatggatataaatggaggaagtatggacagaaatccatcaagaatagcccaaatcctaggagctactacaggtgcacaaacccaagatgcagtgcaaagaagcaagtggagaggtctagtgaggatccggacacactcatcatcacctatgaaggcctccacttacactttgcatatccatttttccaccttacccaggcccagaatccgaatccaccaacgaaaaagcccaagaagagaaccttggcggcacaaccggaggaccatgagcaagaagctcaagaaagcccgcggagcgttactccagacccggaatatgatctgcagcccggcgcatttacggaccaacctcaagagtttgtggaagaagagagggggtcacaagggttgcttgaagatgtggtgccatttatcattaggaagccttcaagtgcttcaaattcttcatgttgctcttaccgttcacctccaacctctcctccttctctgtcatggcctagttactttgatgttgggatcaatcatagcattggatga

Protein Analysis

292

Amino Acids

32.96

Weight (kDa)

5.13

Isoelectric Point (pI)

59.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43290
fragaria_vesca FvH4_6g13470
malus_domestica MD04G1131000.v1.1 MD12G1144100.v1.1
prunus_persica Prupe.6G257500_v2.0.a1
pyrus_communis pycom04g11930 pycom12g13870
rosa_chinensis RchiOBHm_Chr3g0466341
rosa_laevigata RLG00000024584
rosa_multiflora Rmu_sc0003480.1_g000013
rosa_roxburghii Rroxscaffold_6G00414560
rosa_rugosa Rorug03G0081100
rosa_samantha Rh3AG127400 Rh3BG148800 Rh3DG149500
rosa_wichuraiana Rw3G012040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 745
AccI GTMKAC 1 cut(s) 302
AccII CGCG 1 cut(s) 634
AccIII TCCGGA 1 cut(s) 469
AciI CCGC 4 cut(s) 116, 590, 632, 634
AclWI GGATC 3 cut(s) 461, 474, 866
AcoI YGGCCR 1 cut(s) 26
AcsI RAATTY 1 cut(s) 781
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 1 cut(s) 19
AfiI CCNNNNNNNGG 1 cut(s) 538
AgeI ACCGGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 264, 737, 771, 780
AhdI GACNNNNNGTC 1 cut(s) 73
AjnI CCWGG 1 cut(s) 537
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AluBI AGCT 4 cut(s) 49, 61, 405, 620
AluI AGCT 4 cut(s) 49, 61, 405, 620
Alw21I GWGCWC 2 cut(s) 297, 419
Alw26I GTCTC 2 cut(s) 81, 191
Alw44I GTGCAC 1 cut(s) 415
AlwI GGATC 3 cut(s) 461, 474, 866
Aor13HI TCCGGA 1 cut(s) 469
AoxI GGCC 4 cut(s) 26, 496, 540, 836
ApaLI GTGCAC 1 cut(s) 415
ApeKI GCWGC 1 cut(s) 667
ApoI RAATTY 1 cut(s) 781
AsiGI ACCGGT 1 cut(s) 150
AspA2I CCTAGG 1 cut(s) 398
AspLEI GCGC 1 cut(s) 677
AspS9I GGNCC 4 cut(s) 176, 541, 604, 685
AsuC2I CCSGG 2 cut(s) 653, 672
AsuHPI GGTGA 2 cut(s) 478, 798
AsuII TTCGAA 1 cut(s) 131
AvaII GGWCC 3 cut(s) 176, 604, 685
AvrII CCTAGG 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 419
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 745
Bbv12I GWGCWC 2 cut(s) 297, 419
BbvI GCAGC 1 cut(s) 679
BccI CCATC 3 cut(s) 26, 338, 386
BciT130I CCWGG 1 cut(s) 539
BciVI GTATCC 1 cut(s) 123
BcnI CCSGG 2 cut(s) 653, 672
BcoDI GTCTC 2 cut(s) 81, 191
BfaI CTAG 3 cut(s) 399, 459, 840
BfmI CTRYAG 3 cut(s) 105, 409, 665
BfuI GTATCC 1 cut(s) 123
BisI GCNGC 2 cut(s) 591, 668
BlnI CCTAGG 1 cut(s) 398
BlsI GCNGC 2 cut(s) 592, 669
BmcAI AGTACT 1 cut(s) 19
Bme1390I CCNGG 3 cut(s) 539, 653, 672
Bme18I GGWCC 3 cut(s) 176, 604, 685
BmeRI GACNNNNNGTC 1 cut(s) 73
BmgT120I GGNCC 4 cut(s) 176, 541, 604, 685
BmiI GGNNCC 2 cut(s) 468, 747
BmrFI CCNGG 3 cut(s) 539, 653, 672
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 419
BmuI ACTGGG 1 cut(s) 228
BplI GAGNNNNNCTC 2 cut(s) 628, 660
BpmI CTGGAG 1 cut(s) 630
Bpu14I TTCGAA 1 cut(s) 131
BpuEI CTTGAG 2 cut(s) 606, 678
BpuMI CCSGG 2 cut(s) 653, 672
BsaJI CCNNGG 4 cut(s) 398, 537, 585, 632
BsaWI WCCGGW 3 cut(s) 150, 469, 598
BsaXI ACNNNNNCTCC 2 cut(s) 805, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 538
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 1 cut(s) 223
Bse3DI GCAATG 1 cut(s) 18
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 1 cut(s) 539
BseDI CCNNGG 4 cut(s) 398, 537, 585, 632
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 1 cut(s) 538
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 1 cut(s) 223
BseRI GAGGAG 3 cut(s) 59, 113, 810
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 1 cut(s) 679
BsgI GTGCAG 1 cut(s) 179
Bsh1236I CGCG 1 cut(s) 634
BshFI GGCC 4 cut(s) 28, 498, 542, 838
BshNI GGYRCC 1 cut(s) 745
BshTI ACCGGT 1 cut(s) 150
BsiHKAI GWGCWC 2 cut(s) 297, 419
BsiSI CCGG 6 cut(s) 29, 151, 470, 599, 653, 672
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 538
BsmAI GTCTC 2 cut(s) 81, 191
BsmFI GGGAC 1 cut(s) 156
BsnI GGCC 4 cut(s) 28, 498, 542, 838
Bsp119I TTCGAA 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 297, 419
Bsp13I TCCGGA 1 cut(s) 469
Bsp143I GATC 4 cut(s) 312, 466, 661, 858
BspACI CCGC 4 cut(s) 116, 590, 632, 634
BspANI GGCC 4 cut(s) 28, 498, 542, 838
BspEI TCCGGA 1 cut(s) 469
BspFNI CGCG 1 cut(s) 634
BspLI GGNNCC 2 cut(s) 468, 747
BspMAI CTGCAG 1 cut(s) 669
BspPI GGATC 3 cut(s) 461, 474, 866
BspT104I TTCGAA 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 745
BsrDI GCAATG 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 150
BsrI ACTGG 1 cut(s) 223
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 4 cut(s) 398, 537, 585, 632
BssMI GATC 4 cut(s) 312, 466, 661, 858
BssNAI GTATAC 1 cut(s) 303
BssT1I CCWWGG 2 cut(s) 398, 585
Bst1107I GTATAC 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 3 cut(s) 229, 329, 803
Bst6I CTCTTC 3 cut(s) 36, 572, 705
BstBI TTCGAA 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 632
BstDSI CCRYGG 1 cut(s) 632
BstENI CCTNNNNNAGG 1 cut(s) 536
BstF5I GGATG 1 cut(s) 37
BstFNI CGCG 1 cut(s) 634
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 4 cut(s) 315, 469, 664, 861
BstMAI GTCTC 2 cut(s) 81, 191
BstMBI GATC 4 cut(s) 312, 466, 661, 858
BstMWI GCNNNNNNNGC 1 cut(s) 636
BstNI CCWGG 1 cut(s) 539
BstSCI CCNGG 3 cut(s) 537, 651, 670
BstSFI CTRYAG 3 cut(s) 105, 409, 665
BstSLI GKGCMC 1 cut(s) 419
BstUI CGCG 1 cut(s) 634
BstV1I GCAGC 1 cut(s) 679
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BstZ17I GTATAC 1 cut(s) 303
BsuI GTATCC 1 cut(s) 123
BsuRI GGCC 4 cut(s) 28, 498, 542, 838
BtgI CCRYGG 1 cut(s) 632
BtsCI GGATG 1 cut(s) 37
BtsI GCAGTG 1 cut(s) 439
BtsIMutI CAGTG 2 cut(s) 334, 439
Cac8I GCNNGC 1 cut(s) 632
CfoI GCGC 1 cut(s) 677
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 4 cut(s) 176, 541, 604, 685
Cfr42I CCGCGG 1 cut(s) 635
Csp6I GTAC 1 cut(s) 18
CspAI ACCGGT 1 cut(s) 150
CviAII CATG 4 cut(s) 188, 608, 789, 834
CviQI GTAC 1 cut(s) 18
DpnI GATC 4 cut(s) 314, 468, 663, 860
DpnII GATC 4 cut(s) 312, 466, 661, 858
DrdI GACNNNNNNGTC 1 cut(s) 205
DriI GACNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 26
Eam1104I CTCTTC 3 cut(s) 36, 572, 705
Eam1105I GACNNNNNGTC 1 cut(s) 73
EarI CTCTTC 3 cut(s) 36, 572, 705
Eco130I CCWWGG 2 cut(s) 398, 585
Eco147I AGGCCT 1 cut(s) 498
Eco47I GGWCC 3 cut(s) 176, 604, 685
Eco57I CTGAAG 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 536
EcoRII CCWGG 1 cut(s) 537
EcoT14I CCWWGG 2 cut(s) 398, 585
ErhI CCWWGG 2 cut(s) 398, 585
FaeI CATG 4 cut(s) 191, 611, 792, 837
FaqI GGGAC 1 cut(s) 156
FatI CATG 4 cut(s) 187, 607, 788, 833
FauI CCCGC 1 cut(s) 639
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 2 cut(s) 591, 668
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 2 cut(s) 591, 668
FspBI CTAG 3 cut(s) 399, 459, 840
GlaI GCGC 1 cut(s) 676
GluI GCNGC 2 cut(s) 591, 668
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 4 cut(s) 28, 498, 542, 838
HapII CCGG 6 cut(s) 29, 151, 470, 599, 653, 672
HhaI GCGC 1 cut(s) 677
Hin1II CATG 4 cut(s) 191, 611, 792, 837
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HinfI GANTC 3 cut(s) 74, 547, 553
HpaII CCGG 6 cut(s) 29, 151, 470, 599, 653, 672
HphI GGTGA 2 cut(s) 478, 798
Hpy166II GTNNAC 3 cut(s) 303, 417, 806
Hpy188I TCNGA 2 cut(s) 309, 552
Hpy188III TCNNGA 6 cut(s) 174, 382, 470, 623, 647, 695
Hpy8I GTNNAC 3 cut(s) 303, 417, 806
HpyAV CCTTC 3 cut(s) 488, 777, 834
HpyCH4III ACNGT 3 cut(s) 229, 329, 803
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 6 cut(s) 160, 417, 432, 437, 515, 667
HpyF10VI GCNNNNNNNGC 1 cut(s) 636
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 4 cut(s) 191, 611, 792, 837
HspAI GCGC 1 cut(s) 675
Kpn2I TCCGGA 1 cut(s) 469
KspI CCGCGG 1 cut(s) 635
Kzo9I GATC 4 cut(s) 312, 466, 661, 858
LmnI GCTCC 4 cut(s) 46, 58, 402, 636
Lsp1109I GCAGC 1 cut(s) 679
LweI GCATC 1 cut(s) 419
MaeI CTAG 3 cut(s) 399, 459, 840
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 5 cut(s) 211, 323, 640, 720, 842
MalI GATC 4 cut(s) 314, 468, 663, 860
MboI GATC 4 cut(s) 312, 466, 661, 858
MboII GAAGA 7 cut(s) 53, 322, 589, 719, 722, 749, 777
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 2 cut(s) 297, 419
MluCI AATT 2 cut(s) 22, 781
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 3 cut(s) 18, 256, 836
MroI TCCGGA 1 cut(s) 469
MseI TTAA 1 cut(s) 147
MspA1I CMGCKG 1 cut(s) 634
MspI CCGG 6 cut(s) 29, 151, 470, 599, 653, 672
MspR9I CCNGG 3 cut(s) 539, 653, 672
MvaI CCWGG 1 cut(s) 539
MvnI CGCG 1 cut(s) 634
MwoI GCNNNNNNNGC 1 cut(s) 636
NciI CCSGG 2 cut(s) 653, 672
NdeII GATC 4 cut(s) 312, 466, 661, 858
NlaIII CATG 4 cut(s) 191, 611, 792, 837
NlaIV GGNNCC 2 cut(s) 468, 747
NmuCI GTSAC 3 cut(s) 211, 323, 720
NspV TTCGAA 1 cut(s) 131
PceI AGGCCT 1 cut(s) 498
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PfeI GAWTC 2 cut(s) 547, 553
PinAI ACCGGT 1 cut(s) 150
PkrI GCNGC 2 cut(s) 592, 669
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
Psp6I CCWGG 1 cut(s) 537
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 2 cut(s) 468, 747
PspPI GGNCC 4 cut(s) 176, 541, 604, 685
PstI CTGCAG 1 cut(s) 669
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 1 cut(s) 19
RsaNI GTAC 1 cut(s) 18
SacII CCGCGG 1 cut(s) 635
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 2 cut(s) 591, 668
Sau3AI GATC 4 cut(s) 312, 466, 661, 858
Sau96I GGNCC 4 cut(s) 176, 541, 604, 685
ScaI AGTACT 1 cut(s) 19
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 3 cut(s) 539, 653, 672
SduI GDGCHC 2 cut(s) 297, 419
SfaNI GCATC 1 cut(s) 419
SfcI CTRYAG 3 cut(s) 105, 409, 665
Sfr303I CCGCGG 1 cut(s) 635
SfuI TTCGAA 1 cut(s) 131
SgrBI CCGCGG 1 cut(s) 635
SinI GGWCC 3 cut(s) 176, 604, 685
SmlI CTYRAG 2 cut(s) 621, 693
SmoI CTYRAG 2 cut(s) 621, 693
Sse9I AATT 2 cut(s) 22, 781
SseBI AGGCCT 1 cut(s) 498
SsiI CCGC 4 cut(s) 116, 590, 632, 634
SspI AATATT 1 cut(s) 267
SspMI CTAG 3 cut(s) 399, 459, 840
StuI AGGCCT 1 cut(s) 498
StyD4I CCNGG 3 cut(s) 537, 651, 670
StyI CCWWGG 2 cut(s) 398, 585
TaaI ACNGT 3 cut(s) 229, 329, 803
TaiI ACGT 1 cut(s) 168
TaqI TCGA 1 cut(s) 131
TasI AATT 2 cut(s) 22, 781
TatI WGTACW 1 cut(s) 17
TauI GCSGC 1 cut(s) 593
TfiI GAWTC 2 cut(s) 547, 553
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 2 cut(s) 334, 439
TseFI GTSAC 3 cut(s) 211, 323, 720
TseI GCWGC 1 cut(s) 667
Tsp45I GTSAC 3 cut(s) 211, 323, 720
TspDTI ATGAA 2 cut(s) 507, 777
TspGWI ACGGA 2 cut(s) 69, 698
TspRI CASTG 2 cut(s) 334, 439
VneI GTGCAC 1 cut(s) 415
VpaK11BI GGWCC 3 cut(s) 176, 604, 685
XagI CCTNNNNNAGG 1 cut(s) 536
XapI RAATTY 1 cut(s) 781
XmaJI CCTAGG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 302
XspI CTAG 3 cut(s) 399, 459, 840
ZrmI AGTACT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.