MD04G1131000.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015149
Physical Location & Seq
Reverse (-)
21800375 .. 21802338
1964 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1131000.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGAGGAATCAATGGCTGCTACGTGGTCGGACTGGTCGGAGGAAGAGCTGGTGAGGGAGCTTCTGGACAATGATTCACCACTGTTTGTGCTACCGGAGGAGGCTGTGCAGTCCCGAGCGGCTAGTGTTTTCGGTAAAGAGGCTATCAACCGATTCATCCCTAACGTCTATTCGGGTCCAACGATCCAAGATATCGAGACTGCTTTGTCTGTCACAACTGGAACAGTCCAACCCCAAGAACTTTCAAACGCCAGGCTGTCATTGCTAGAAAGGGGTCTGAGTAAGGTTGAGCATAAGTACACTCTGAAGATCAAAAGCTGTGGAAATGGAATAATGGCTGATGATGGTTATAAATGGAGGAAGTATGGCCAGAAATCTATTAAGAATAGCCCTAATCCTAGGAGCTACTACCGGTGCACAAATCCAAGATGCAGTGCAAAAAAGCAAGTAGAGAGGTCCAGTGAGGACCCAGACACACTCATCATCACCTATGAAGGGCTCCATTTACACTTTGCATACCCATTTTTCAACCTCAACAACCAGGCCCAAAACTCCACTCCACCAATGAAGAAGACCAAGAAGAAAACCTTGGATCCCCAACCCGAAGACCAAGAACGAGAAGCACAAGAGAGCCCGCGAAGCCTTACTCCCGATCCAGTTCCAGATCTGCCGCCCGGCCCGTTTCCGGACCGGTATCAAGAGTTTGTGAAAGAAGAGAGAAGCTCACAAGGGTTGCTTCAAGATATGGTGCCATTCATGATTAGGAACCCTAAACCAACGAGTGCTTCTTCAAACTCTTCATGTTCCTCTTATCGTTCATCTCCAACGTCTCCTTCTTCTCTATCATGGGCCACTTCCTATTTTGACGTTGGCATAAATCATAGTTTTGGATGA

Protein Analysis

298

Amino Acids

33.41

Weight (kDa)

5.72

Isoelectric Point (pI)

53.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 114 - 170 7e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43290
fragaria_vesca FvH4_6g13470
malus_domestica MD04G1131000.v1.1 MD12G1144100.v1.1
prunus_persica Prupe.6G257500_v2.0.a1
pyrus_communis pycom04g11930 pycom12g13870
rosa_chinensis RchiOBHm_Chr3g0466341
rosa_laevigata RLG00000024584
rosa_multiflora Rmu_sc0003480.1_g000013
rosa_roxburghii Rroxscaffold_6G00414560
rosa_rugosa Rorug03G0081100
rosa_samantha Rh3AG127400 Rh3BG148800 Rh3DG149500
rosa_wichuraiana Rw3G012040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AasI GACNNNNNNGTC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 748
AccBSI CCGCTC 1 cut(s) 119
AccII CGCG 1 cut(s) 637
AccIII TCCGGA 1 cut(s) 685
AciI CCGC 3 cut(s) 119, 635, 671
AclWI GGATC 4 cut(s) 178, 587, 600, 647
AcoI YGGCCR 1 cut(s) 367
AcuI CTGAAG 1 cut(s) 326
AfaI GTAC 1 cut(s) 299
AfiI CCNNNNNNNGG 2 cut(s) 495, 685
AgeI ACCGGT 2 cut(s) 411, 690
AgsI TTSAA 4 cut(s) 246, 529, 740, 792
AjnI CCWGG 2 cut(s) 251, 540
AjuI GAANNNNNNNTTGG 6 cut(s) 572, 604, 769, 801, 817, 849
AluBI AGCT 5 cut(s) 49, 61, 318, 405, 723
AluI AGCT 5 cut(s) 49, 61, 318, 405, 723
Alw21I GWGCWC 1 cut(s) 419
Alw26I GTCTC 2 cut(s) 191, 834
Alw44I GTGCAC 1 cut(s) 415
AlwI GGATC 4 cut(s) 178, 587, 600, 647
Ama87I CYCGRG 1 cut(s) 114
Aor13HI TCCGGA 1 cut(s) 685
AoxI GGCC 4 cut(s) 367, 543, 676, 849
ApaLI GTGCAC 1 cut(s) 415
ApeKI GCWGC 1 cut(s) 17
AsiGI ACCGGT 2 cut(s) 411, 690
AspA2I CCTAGG 1 cut(s) 398
AspS9I GGNCC 7 cut(s) 176, 456, 466, 544, 677, 688, 849
AsuC2I CCSGG 1 cut(s) 675
AsuHPI GGTGA 3 cut(s) 64, 69, 478
AvaI CYCGRG 1 cut(s) 114
AvaII GGWCC 4 cut(s) 176, 456, 466, 688
AvrII CCTAGG 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 419
BalI TGGCCA 1 cut(s) 369
BamHI GGATCC 1 cut(s) 592
BanI GGYRCC 1 cut(s) 748
BanII GRGCYC 2 cut(s) 501, 635
BbsI GAAGAC 2 cut(s) 578, 612
Bbv12I GWGCWC 1 cut(s) 419
BbvI GCAGC 1 cut(s) 4
BccI CCATC 1 cut(s) 338
BciT130I CCWGG 2 cut(s) 253, 542
BcnI CCSGG 1 cut(s) 675
BcoDI GTCTC 2 cut(s) 191, 834
BfaI CTAG 3 cut(s) 123, 266, 399
BglII AGATCT 1 cut(s) 664
BisI GCNGC 3 cut(s) 18, 120, 671
BlnI CCTAGG 1 cut(s) 398
BlsI GCNGC 3 cut(s) 19, 121, 672
Bme1390I CCNGG 3 cut(s) 253, 542, 675
Bme18I GGWCC 4 cut(s) 176, 456, 466, 688
BmeT110I CYCGRG 1 cut(s) 114
BmgT120I GGNCC 7 cut(s) 176, 456, 466, 544, 677, 688, 849
BmiI GGNNCC 6 cut(s) 177, 468, 500, 594, 750, 767
BmrFI CCNGG 3 cut(s) 253, 542, 675
BmsI GCATC 1 cut(s) 419
BpiI GAAGAC 2 cut(s) 578, 612
BplI GAGNNNNNCTC 2 cut(s) 707, 739
BpuMI CCSGG 1 cut(s) 675
BsaAI YACGTR 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 398, 588
BsaWI WCCGGW 4 cut(s) 94, 411, 685, 690
Bsc4I CCNNNNNNNGG 2 cut(s) 495, 685
Bse118I RCCGGY 2 cut(s) 411, 690
Bse1I ACTGG 4 cut(s) 38, 223, 459, 656
Bse3DI GCAATG 1 cut(s) 260
BseAI TCCGGA 1 cut(s) 685
BseBI CCWGG 2 cut(s) 253, 542
BseDI CCNNGG 2 cut(s) 398, 588
BseGI GGATG 1 cut(s) 156
BseLI CCNNNNNNNGG 2 cut(s) 495, 685
BseMI GCAATG 1 cut(s) 260
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 4 cut(s) 38, 223, 459, 656
BseRI GAGGAG 1 cut(s) 113
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 1 cut(s) 4
BsgI GTGCAG 1 cut(s) 128
Bsh1236I CGCG 1 cut(s) 637
BshFI GGCC 4 cut(s) 369, 545, 678, 851
BshNI GGYRCC 1 cut(s) 748
BshTI ACCGGT 2 cut(s) 411, 690
BsiHKAI GWGCWC 1 cut(s) 419
BsiHKCI CYCGRG 1 cut(s) 114
BsiSI CCGG 5 cut(s) 95, 412, 675, 686, 691
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 2 cut(s) 495, 685
BsmAI GTCTC 2 cut(s) 191, 834
BsmBI CGTCTC 1 cut(s) 834
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 4 cut(s) 369, 545, 678, 851
BsoBI CYCGRG 1 cut(s) 114
Bsp1286I GDGCHC 3 cut(s) 419, 501, 635
Bsp13I TCCGGA 1 cut(s) 685
Bsp143I GATC 5 cut(s) 183, 309, 592, 652, 664
BspACI CCGC 3 cut(s) 119, 635, 671
BspANI GGCC 4 cut(s) 369, 545, 678, 851
BspCNI CTCAG 1 cut(s) 270
BspEI TCCGGA 1 cut(s) 685
BspFNI CGCG 1 cut(s) 637
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 6 cut(s) 177, 468, 500, 594, 750, 767
BspPI GGATC 4 cut(s) 178, 587, 600, 647
BspQI GCTCTTC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 748
BsrBI CCGCTC 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 260
BsrFI RCCGGY 2 cut(s) 411, 690
BsrI ACTGG 4 cut(s) 38, 223, 459, 656
BssAI RCCGGY 2 cut(s) 411, 690
BssECI CCNNGG 2 cut(s) 398, 588
BssMI GATC 5 cut(s) 183, 309, 592, 652, 664
BssT1I CCWWGG 2 cut(s) 398, 588
Bst2UI CCWGG 2 cut(s) 253, 542
Bst4CI ACNGT 2 cut(s) 84, 226
Bst6I CTCTTC 3 cut(s) 39, 708, 802
BstBAI YACGTR 1 cut(s) 24
BstC8I GCNNGC 1 cut(s) 635
BstDEI CTNAG 1 cut(s) 278
BstF5I GGATG 1 cut(s) 156
BstFNI CGCG 1 cut(s) 637
BstKTI GATC 5 cut(s) 186, 312, 595, 655, 667
BstMAI GTCTC 2 cut(s) 191, 834
BstMBI GATC 5 cut(s) 183, 309, 592, 652, 664
BstMWI GCNNNNNNNGC 2 cut(s) 262, 639
BstNI CCWGG 2 cut(s) 253, 542
BstSCI CCNGG 3 cut(s) 251, 540, 673
BstSLI GKGCMC 1 cut(s) 419
BstUI CGCG 1 cut(s) 637
BstV1I GCAGC 1 cut(s) 4
BstV2I GAAGAC 2 cut(s) 578, 612
BstX2I RGATCY 2 cut(s) 592, 664
BstYI RGATCY 2 cut(s) 592, 664
BsuRI GGCC 4 cut(s) 369, 545, 678, 851
BtsCI GGATG 1 cut(s) 156
BtsI GCAGTG 1 cut(s) 439
BtsIMutI CAGTG 3 cut(s) 80, 439, 466
Cac8I GCNNGC 1 cut(s) 635
CciI TCATGA 1 cut(s) 756
Cfr10I RCCGGY 2 cut(s) 411, 690
Cfr13I GGNCC 7 cut(s) 176, 456, 466, 544, 677, 688, 849
CpoI CGGWCCG 1 cut(s) 688
Csp6I GTAC 1 cut(s) 298
CspAI ACCGGT 2 cut(s) 411, 690
CspCI CAANNNNNGTGG 2 cut(s) 301, 336
CspI CGGWCCG 1 cut(s) 688
CviAII CATG 3 cut(s) 757, 801, 846
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 1 cut(s) 278
DpnI GATC 5 cut(s) 185, 311, 594, 654, 666
DpnII GATC 5 cut(s) 183, 309, 592, 652, 664
DrdI GACNNNNNNGTC 1 cut(s) 205
DseDI GACNNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 367
Eam1104I CTCTTC 3 cut(s) 39, 708, 802
EarI CTCTTC 3 cut(s) 39, 708, 802
Eco130I CCWWGG 2 cut(s) 398, 588
Eco24I GRGCYC 2 cut(s) 501, 635
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 4 cut(s) 176, 456, 466, 688
Eco57I CTGAAG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 114
EcoO109I RGGNCCY 1 cut(s) 466
EcoRII CCWGG 2 cut(s) 251, 540
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 398, 588
EcoT38I GRGCYC 2 cut(s) 501, 635
ErhI CCWWGG 2 cut(s) 398, 588
Esp3I CGTCTC 1 cut(s) 834
FaeI CATG 3 cut(s) 760, 804, 849
FalI AAGNNNNNCTT 4 cut(s) 572, 604, 720, 752
FaqI GGGAC 1 cut(s) 97
FatI CATG 3 cut(s) 756, 800, 845
FauI CCCGC 1 cut(s) 642
Fnu4HI GCNGC 3 cut(s) 18, 120, 671
FokI GGATG 1 cut(s) 143
FriOI GRGCYC 2 cut(s) 501, 635
Fsp4HI GCNGC 3 cut(s) 18, 120, 671
FspBI CTAG 3 cut(s) 123, 266, 399
GluI GCNGC 3 cut(s) 18, 120, 671
HaeIII GGCC 4 cut(s) 369, 545, 678, 851
HapII CCGG 5 cut(s) 95, 412, 675, 686, 691
Hin1II CATG 3 cut(s) 760, 804, 849
HinfI GANTC 3 cut(s) 8, 74, 153
HpaII CCGG 5 cut(s) 95, 412, 675, 686, 691
HphI GGTGA 3 cut(s) 64, 69, 478
Hpy166II GTNNAC 2 cut(s) 300, 417
Hpy188I TCNGA 4 cut(s) 31, 40, 279, 306
Hpy188III TCNNGA 9 cut(s) 65, 114, 196, 650, 662, 686, 698, 740, 757
Hpy8I GTNNAC 2 cut(s) 300, 417
HpyAV CCTTC 2 cut(s) 488, 843
HpyCH4III ACNGT 2 cut(s) 84, 226
HpyCH4IV ACGT 4 cut(s) 23, 165, 827, 867
HpyCH4V TGCA 5 cut(s) 109, 417, 432, 437, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 262, 639
HpyF3I CTNAG 1 cut(s) 278
HpySE526I ACGT 4 cut(s) 23, 165, 827, 867
Hsp92II CATG 3 cut(s) 760, 804, 849
Kpn2I TCCGGA 1 cut(s) 685
Kzo9I GATC 5 cut(s) 183, 309, 592, 652, 664
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 3 cut(s) 58, 402, 504
Lsp1109I GCAGC 1 cut(s) 4
LweI GCATC 1 cut(s) 419
MaeI CTAG 3 cut(s) 123, 266, 399
MaeII ACGT 4 cut(s) 23, 165, 827, 867
MaeIII GTNAC 1 cut(s) 211
MalI GATC 5 cut(s) 185, 311, 594, 654, 666
MbiI CCGCTC 1 cut(s) 119
MboI GATC 5 cut(s) 183, 309, 592, 652, 664
MflI RGATCY 2 cut(s) 592, 664
MhlI GDGCHC 3 cut(s) 419, 501, 635
MlsI TGGCCA 1 cut(s) 369
MluNI TGGCCA 1 cut(s) 369
MmeI TCCRAC 5 cut(s) 9, 18, 203, 253, 848
Mox20I TGGCCA 1 cut(s) 369
MroI TCCGGA 1 cut(s) 685
MscI TGGCCA 1 cut(s) 369
MseI TTAA 1 cut(s) 381
Msp20I TGGCCA 1 cut(s) 369
MspI CCGG 5 cut(s) 95, 412, 675, 686, 691
MspR9I CCNGG 3 cut(s) 253, 542, 675
MvaI CCWGG 2 cut(s) 253, 542
MvnI CGCG 1 cut(s) 637
MwoI GCNNNNNNNGC 2 cut(s) 262, 639
NciI CCSGG 1 cut(s) 675
NdeII GATC 5 cut(s) 183, 309, 592, 652, 664
NlaIII CATG 3 cut(s) 760, 804, 849
NlaIV GGNNCC 6 cut(s) 177, 468, 500, 594, 750, 767
NmuCI GTSAC 1 cut(s) 211
PagI TCATGA 1 cut(s) 756
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 179
PfeI GAWTC 3 cut(s) 8, 74, 153
PinAI ACCGGT 2 cut(s) 411, 690
PkrI GCNGC 3 cut(s) 19, 121, 672
Ppu21I YACGTR 1 cut(s) 24
PpuMI RGGWCCY 1 cut(s) 466
PsiI TTATAA 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 466
Psp6I CCWGG 2 cut(s) 251, 540
PspGI CCWGG 2 cut(s) 251, 540
PspN4I GGNNCC 6 cut(s) 177, 468, 500, 594, 750, 767
PspPI GGNCC 7 cut(s) 176, 456, 466, 544, 677, 688, 849
PspPPI RGGWCCY 1 cut(s) 466
PsuI RGATCY 2 cut(s) 592, 664
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
Rsr2I CGGWCCG 1 cut(s) 688
RsrII CGGWCCG 1 cut(s) 688
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 3 cut(s) 18, 120, 671
Sau3AI GATC 5 cut(s) 183, 309, 592, 652, 664
Sau96I GGNCC 7 cut(s) 176, 456, 466, 544, 677, 688, 849
ScrFI CCNGG 3 cut(s) 253, 542, 675
SduI GDGCHC 3 cut(s) 419, 501, 635
SfaNI GCATC 1 cut(s) 419
SinI GGWCC 4 cut(s) 176, 456, 466, 688
SsiI CCGC 3 cut(s) 119, 635, 671
SspMI CTAG 3 cut(s) 123, 266, 399
StyD4I CCNGG 3 cut(s) 251, 540, 673
StyI CCWWGG 2 cut(s) 398, 588
TaaI ACNGT 2 cut(s) 84, 226
TaiI ACGT 4 cut(s) 26, 168, 830, 870
TaqI TCGA 1 cut(s) 195
TatI WGTACW 1 cut(s) 297
TauI GCSGC 2 cut(s) 122, 673
TfiI GAWTC 3 cut(s) 8, 74, 153
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 3 cut(s) 87, 439, 466
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 6 cut(s) 145, 507, 581, 745, 789, 807
TspRI CASTG 3 cut(s) 87, 439, 466
VneI GTGCAC 1 cut(s) 415
VpaK11BI GGWCC 4 cut(s) 176, 456, 466, 688
XmaJI CCTAGG 1 cut(s) 398
XspI CTAG 3 cut(s) 123, 266, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.