Rh3AG127400
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0015149
Physical Location & Seq
Forward (+)
10993986 .. 10996214
2229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG127400.1

Sequence Viewer

Length: 645 bp
ATGTATCTTGTTTTTAAGTGCAGACTTTCAATATTGGAGAGAGGTTTGAGTAAGGTTGAGCACAAGTATACTCTGAAGATCAAAAGTTGTGACAGTGGAATGGCTGATGATGGATATAAATGGAGGAAGTATGGACAGAAATCCATCAAGAATAGCCCAAATCCTAGGAGCTACTACAGGTGCACAAACCCAAGATGCAGTGCAAAGAAGCAAGTGGAGAGGTCTAGTGAGGATCCGGACACACTCATCATCACCTATGAAGGCCTCCACTTACACTTTGCATATCCATTTTTCCACCTTACCCCGGCCCAGAATCCGAATCCACCAACGAAAAAGCCCAAGAAGAGAACCTTGGCGGCACAACCGGAGGACCATGAGCAAGATGCTCAAGAAAGCCCGCGGAGCGTTACTCCAGACCCGGAACATGATCTGCAGCCCGGCGCATTTACGGATCAACCTCAAGAGTTTGTGGAAGAAGAGAGGGGGTCACAAGGGTTGCTTGAAGATGTGGTGCCATTTATGATTAGGAAGCCTTCAAGTGCTTCAAATTCTTCATGTTGCTCTTACCGTTCACCTCCAACCTCTCCTCCTTCTCTGTCATGGCCTAGTTACTTTGATGTTGGGATCAATCATAGCATTGGATGA

Protein Analysis

214

Amino Acids

24.28

Weight (kDa)

8.15

Isoelectric Point (pI)

56.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 36 - 92 3.9e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43290
fragaria_vesca FvH4_6g13470
malus_domestica MD04G1131000.v1.1 MD12G1144100.v1.1
prunus_persica Prupe.6G257500_v2.0.a1
pyrus_communis pycom04g11930 pycom12g13870
rosa_chinensis RchiOBHm_Chr3g0466341
rosa_laevigata RLG00000024584
rosa_multiflora Rmu_sc0003480.1_g000013
rosa_roxburghii Rroxscaffold_6G00414560
rosa_rugosa Rorug03G0081100
rosa_samantha Rh3AG127400 Rh3BG148800 Rh3DG149500
rosa_wichuraiana Rw3G012040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 511
AccI GTMKAC 1 cut(s) 68
AccII CGCG 1 cut(s) 400
AccIII TCCGGA 1 cut(s) 235
AciI CCGC 3 cut(s) 356, 398, 400
AclWI GGATC 4 cut(s) 227, 240, 459, 632
AcsI RAATTY 1 cut(s) 547
AcuI CTGAAG 1 cut(s) 95
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 4 cut(s) 30, 503, 537, 546
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AluBI AGCT 1 cut(s) 171
AluI AGCT 1 cut(s) 171
Alw21I GWGCWC 2 cut(s) 63, 185
Alw44I GTGCAC 1 cut(s) 181
AlwI GGATC 4 cut(s) 227, 240, 459, 632
Aor13HI TCCGGA 1 cut(s) 235
AoxI GGCC 3 cut(s) 262, 306, 602
ApaLI GTGCAC 1 cut(s) 181
ApeKI GCWGC 1 cut(s) 433
ApoI RAATTY 1 cut(s) 547
AspA2I CCTAGG 1 cut(s) 164
AspLEI GCGC 1 cut(s) 443
AspS9I GGNCC 2 cut(s) 307, 370
AsuC2I CCSGG 3 cut(s) 305, 419, 438
AsuHPI GGTGA 2 cut(s) 244, 564
AvaII GGWCC 1 cut(s) 370
AvrII CCTAGG 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 185
BamHI GGATCC 1 cut(s) 232
BanI GGYRCC 1 cut(s) 511
Bbv12I GWGCWC 2 cut(s) 63, 185
BbvI GCAGC 1 cut(s) 445
BccI CCATC 2 cut(s) 104, 152
BcnI CCSGG 3 cut(s) 305, 419, 438
BfaI CTAG 3 cut(s) 165, 225, 606
BfmI CTRYAG 2 cut(s) 175, 431
BisI GCNGC 2 cut(s) 357, 434
BlnI CCTAGG 1 cut(s) 164
BlsI GCNGC 2 cut(s) 358, 435
Bme1390I CCNGG 3 cut(s) 305, 419, 438
Bme18I GGWCC 1 cut(s) 370
BmgT120I GGNCC 2 cut(s) 307, 370
BmiI GGNNCC 2 cut(s) 234, 513
BmrFI CCNGG 3 cut(s) 305, 419, 438
BmsI GCATC 2 cut(s) 185, 373
BplI GAGNNNNNCTC 2 cut(s) 394, 426
BpmI CTGGAG 1 cut(s) 396
BpuEI CTTGAG 2 cut(s) 372, 444
BpuMI CCSGG 3 cut(s) 305, 419, 438
BsaJI CCNNGG 4 cut(s) 164, 303, 351, 398
BsaWI WCCGGW 2 cut(s) 235, 364
BsaXI ACNNNNNCTCC 2 cut(s) 571, 601
Bsc4I CCNNNNNNNGG 1 cut(s) 304
BseAI TCCGGA 1 cut(s) 235
BseDI CCNNGG 4 cut(s) 164, 303, 351, 398
BseLI CCNNNNNNNGG 1 cut(s) 304
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 1 cut(s) 445
BsgI GTGCAG 1 cut(s) 40
Bsh1236I CGCG 1 cut(s) 400
BshFI GGCC 3 cut(s) 264, 308, 604
BshNI GGYRCC 1 cut(s) 511
BsiHKAI GWGCWC 2 cut(s) 63, 185
BsiSI CCGG 5 cut(s) 236, 305, 365, 419, 438
BslI CCNNNNNNNGG 1 cut(s) 304
BsnI GGCC 3 cut(s) 264, 308, 604
Bsp1286I GDGCHC 2 cut(s) 63, 185
Bsp13I TCCGGA 1 cut(s) 235
Bsp143I GATC 5 cut(s) 78, 232, 427, 451, 624
BspACI CCGC 3 cut(s) 356, 398, 400
BspANI GGCC 3 cut(s) 264, 308, 604
BspEI TCCGGA 1 cut(s) 235
BspFNI CGCG 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 234, 513
BspMAI CTGCAG 1 cut(s) 435
BspPI GGATC 4 cut(s) 227, 240, 459, 632
BspT107I GGYRCC 1 cut(s) 511
BssECI CCNNGG 4 cut(s) 164, 303, 351, 398
BssMI GATC 5 cut(s) 78, 232, 427, 451, 624
BssNAI GTATAC 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 164, 351
Bst1107I GTATAC 1 cut(s) 69
Bst4CI ACNGT 2 cut(s) 95, 569
Bst6I CTCTTC 2 cut(s) 338, 471
BstC8I GCNNGC 1 cut(s) 398
BstDSI CCRYGG 1 cut(s) 398
BstFNI CGCG 1 cut(s) 400
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 5 cut(s) 81, 235, 430, 454, 627
BstMBI GATC 5 cut(s) 78, 232, 427, 451, 624
BstMWI GCNNNNNNNGC 1 cut(s) 402
BstSCI CCNGG 3 cut(s) 303, 417, 436
BstSFI CTRYAG 2 cut(s) 175, 431
BstSLI GKGCMC 1 cut(s) 185
BstUI CGCG 1 cut(s) 400
BstV1I GCAGC 1 cut(s) 445
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
BstZ17I GTATAC 1 cut(s) 69
BsuRI GGCC 3 cut(s) 264, 308, 604
BtgI CCRYGG 1 cut(s) 398
BtsI GCAGTG 1 cut(s) 205
BtsIMutI CAGTG 2 cut(s) 100, 205
Cac8I GCNNGC 1 cut(s) 398
CfoI GCGC 1 cut(s) 443
Cfr13I GGNCC 2 cut(s) 307, 370
Cfr42I CCGCGG 1 cut(s) 401
CviAII CATG 4 cut(s) 374, 425, 555, 600
DpnI GATC 5 cut(s) 80, 234, 429, 453, 626
DpnII GATC 5 cut(s) 78, 232, 427, 451, 624
Eam1104I CTCTTC 2 cut(s) 338, 471
EarI CTCTTC 2 cut(s) 338, 471
Eco130I CCWWGG 2 cut(s) 164, 351
Eco147I AGGCCT 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 95
EcoT14I CCWWGG 2 cut(s) 164, 351
ErhI CCWWGG 2 cut(s) 164, 351
FaeI CATG 4 cut(s) 377, 428, 558, 603
FalI AAGNNNNNCTT 4 cut(s) 335, 367, 483, 515
FatI CATG 4 cut(s) 373, 424, 554, 599
FauI CCCGC 1 cut(s) 405
FblI GTMKAC 1 cut(s) 68
Fnu4HI GCNGC 2 cut(s) 357, 434
Fsp4HI GCNGC 2 cut(s) 357, 434
FspBI CTAG 3 cut(s) 165, 225, 606
GlaI GCGC 1 cut(s) 442
GluI GCNGC 2 cut(s) 357, 434
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 3 cut(s) 264, 308, 604
HapII CCGG 5 cut(s) 236, 305, 365, 419, 438
HhaI GCGC 1 cut(s) 443
Hin1II CATG 4 cut(s) 377, 428, 558, 603
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HinfI GANTC 2 cut(s) 313, 319
HpaII CCGG 5 cut(s) 236, 305, 365, 419, 438
HphI GGTGA 2 cut(s) 244, 564
Hpy166II GTNNAC 3 cut(s) 69, 183, 572
Hpy188I TCNGA 2 cut(s) 75, 318
Hpy188III TCNNGA 5 cut(s) 148, 236, 389, 413, 461
Hpy8I GTNNAC 3 cut(s) 69, 183, 572
HpyAV CCTTC 3 cut(s) 254, 543, 600
HpyCH4III ACNGT 2 cut(s) 95, 569
HpyCH4V TGCA 6 cut(s) 21, 183, 198, 203, 281, 433
HpyF10VI GCNNNNNNNGC 1 cut(s) 402
Hsp92II CATG 4 cut(s) 377, 428, 558, 603
HspAI GCGC 1 cut(s) 441
Kpn2I TCCGGA 1 cut(s) 235
KspI CCGCGG 1 cut(s) 401
Kzo9I GATC 5 cut(s) 78, 232, 427, 451, 624
LmnI GCTCC 2 cut(s) 168, 402
LpnPI CCDG 8 cut(s) 163, 249, 318, 323, 378, 426, 432, 451
Lsp1109I GCAGC 1 cut(s) 445
LweI GCATC 2 cut(s) 185, 373
MaeI CTAG 3 cut(s) 165, 225, 606
MaeIII GTNAC 4 cut(s) 89, 406, 486, 608
MalI GATC 5 cut(s) 80, 234, 429, 453, 626
MboI GATC 5 cut(s) 78, 232, 427, 451, 624
MboII GAAGA 6 cut(s) 88, 355, 485, 488, 515, 543
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 2 cut(s) 63, 185
MluCI AATT 1 cut(s) 547
MmeI TCCRAC 1 cut(s) 602
MroI TCCGGA 1 cut(s) 235
MseI TTAA 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 400
MspI CCGG 5 cut(s) 236, 305, 365, 419, 438
MspR9I CCNGG 3 cut(s) 305, 419, 438
MvnI CGCG 1 cut(s) 400
MwoI GCNNNNNNNGC 1 cut(s) 402
NciI CCSGG 3 cut(s) 305, 419, 438
NdeII GATC 5 cut(s) 78, 232, 427, 451, 624
NlaIII CATG 4 cut(s) 377, 428, 558, 603
NlaIV GGNNCC 2 cut(s) 234, 513
NmuCI GTSAC 2 cut(s) 89, 486
PceI AGGCCT 1 cut(s) 264
PfeI GAWTC 2 cut(s) 313, 319
PkrI GCNGC 2 cut(s) 358, 435
PspN4I GGNNCC 2 cut(s) 234, 513
PspPI GGNCC 2 cut(s) 307, 370
PstI CTGCAG 1 cut(s) 435
PsuI RGATCY 1 cut(s) 232
SacII CCGCGG 1 cut(s) 401
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 2 cut(s) 357, 434
Sau3AI GATC 5 cut(s) 78, 232, 427, 451, 624
Sau96I GGNCC 2 cut(s) 307, 370
ScrFI CCNGG 3 cut(s) 305, 419, 438
SduI GDGCHC 2 cut(s) 63, 185
SfaNI GCATC 2 cut(s) 185, 373
SfcI CTRYAG 2 cut(s) 175, 431
Sfr303I CCGCGG 1 cut(s) 401
SgrBI CCGCGG 1 cut(s) 401
SinI GGWCC 1 cut(s) 370
SmlI CTYRAG 2 cut(s) 387, 459
SmoI CTYRAG 2 cut(s) 387, 459
Sse9I AATT 1 cut(s) 547
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 3 cut(s) 356, 398, 400
SspI AATATT 1 cut(s) 33
SspMI CTAG 3 cut(s) 165, 225, 606
StuI AGGCCT 1 cut(s) 264
StyD4I CCNGG 3 cut(s) 303, 417, 436
StyI CCWWGG 2 cut(s) 164, 351
TaaI ACNGT 2 cut(s) 95, 569
TasI AATT 1 cut(s) 547
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 2 cut(s) 313, 319
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 2 cut(s) 100, 205
TseFI GTSAC 2 cut(s) 89, 486
TseI GCWGC 1 cut(s) 433
Tsp45I GTSAC 2 cut(s) 89, 486
TspDTI ATGAA 2 cut(s) 273, 543
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 2 cut(s) 100, 205
VneI GTGCAC 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 370
XapI RAATTY 1 cut(s) 547
XmaJI CCTAGG 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 68
XspI CTAG 3 cut(s) 165, 225, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.