FvH4_6g13470
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015149
Physical Location & Seq
Forward (+)
8173135 .. 8175309
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13470.t1

Sequence Viewer

Length: 891 bp
ATGGAGGAAGCAATGAGTACTAATTGGACGGTTGGTTTCGAAGAGGAGCTTGTGAGGGAGCTTCTGGACAACGAGTCTCCTTTCTTTGTGTTACCAGAGGAGGCTATAGAGTATCCGCAAATGAGTGTTTCAAGCAAAGAGGGACTAAACCGGTTTGTGCAGAACGTCTATTCTGGACCAACAATCCATGATATCGAGACCGCTTTGTCTGTCACAACTGGGATAACAGTCCAACCCCAAGAACTATCACAAGCCAGGCTTTCAATATTGGAGAGGGGCTTGAGTAAGGTTGAGCACAAGTATACTCTGAAGATCAAAAGTTGTGACACTGGAATGGCTGATGATGGATATAAATGGAGAAAGTATGGACAGAAATCCATCAAGAATAGCCCAAATCCTAGGAGCTACTACAGGTGCACAAACCCAAGATGCAGTGCAAAGAAGCAAGTGGAGAGGTCAAGTGAAGATCCAGACACACTCATCATCACCTATGAAGGCCTCCACCTACACTTTGCATATCCATTTTTCCACCTAACCCAGGCCCAGAACCCGAATCCACCGACCAAAAAACCCAAGAAGAAAACCTCACCAGCCCAACCCGAGGACCACCACGAGCAAGTAGCTCAAGAAAGCTCGCGTAGCGTTACTCCAGACCCGGAACCTGATCTGCATCCCGGCGGCACGTATGCGGACCAACCACCTCAAGAGTTCGTAGAAGAAGAGAGGGGGTCACAAGGTCTGCTTGAAGATGTGGTGCCATTTATGATTAGGAAACCTTCAAGTGCTTCTTCAAATTCTTCTTGTTGCTCTTACCGTTCACCTCCAACCTCCCCTTCATCTTATTCATGGCCTAGTTATTTTGATGTTGGGATCAATCATAGCATTGGCTGA

Protein Analysis

297

Amino Acids

33.31

Weight (kDa)

5.44

Isoelectric Point (pI)

58.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 114 - 170 6.9e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43290
fragaria_vesca FvH4_6g13470
malus_domestica MD04G1131000.v1.1 MD12G1144100.v1.1
prunus_persica Prupe.6G257500_v2.0.a1
pyrus_communis pycom04g11930 pycom12g13870
rosa_chinensis RchiOBHm_Chr3g0466341
rosa_laevigata RLG00000024584
rosa_multiflora Rmu_sc0003480.1_g000013
rosa_roxburghii Rroxscaffold_6G00414560
rosa_rugosa Rorug03G0081100
rosa_samantha Rh3AG127400 Rh3BG148800 Rh3DG149500
rosa_wichuraiana Rw3G012040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 754
AccI GTMKAC 1 cut(s) 302
AccII CGCG 1 cut(s) 637
AciI CCGC 4 cut(s) 116, 201, 678, 689
AclWI GGATC 2 cut(s) 461, 878
AcsI RAATTY 1 cut(s) 793
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 1 cut(s) 19
AfiI CCNNNNNNNGG 2 cut(s) 538, 601
AgeI ACCGGT 1 cut(s) 150
AgsI TTSAA 5 cut(s) 132, 264, 746, 780, 792
AhdI GACNNNNNGTC 1 cut(s) 73
AjnI CCWGG 2 cut(s) 254, 537
AjuI GAANNNNNNNTTGG 2 cut(s) 817, 849
AluBI AGCT 5 cut(s) 49, 61, 405, 623, 633
AluI AGCT 5 cut(s) 49, 61, 405, 623, 633
Alw21I GWGCWC 2 cut(s) 297, 419
Alw26I GTCTC 2 cut(s) 81, 191
Alw44I GTGCAC 1 cut(s) 415
AlwI GGATC 2 cut(s) 461, 878
Ama87I CYCGRG 1 cut(s) 599
AoxI GGCC 3 cut(s) 496, 540, 848
ApaLI GTGCAC 1 cut(s) 415
ApoI RAATTY 1 cut(s) 793
AsiGI ACCGGT 1 cut(s) 150
AspA2I CCTAGG 1 cut(s) 398
AspS9I GGNCC 4 cut(s) 176, 541, 604, 691
AsuC2I CCSGG 2 cut(s) 656, 675
AsuHPI GGTGA 3 cut(s) 478, 579, 810
AsuII TTCGAA 1 cut(s) 39
AvaI CYCGRG 1 cut(s) 599
AvaII GGWCC 3 cut(s) 176, 604, 691
AvrII CCTAGG 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 419
BanI GGYRCC 1 cut(s) 754
BauI CACGAG 1 cut(s) 611
Bbv12I GWGCWC 2 cut(s) 297, 419
BccI CCATC 2 cut(s) 338, 386
BciT130I CCWGG 2 cut(s) 256, 539
BciVI GTATCC 1 cut(s) 123
BcnI CCSGG 2 cut(s) 656, 675
BcoDI GTCTC 2 cut(s) 81, 191
BfaI CTAG 2 cut(s) 399, 852
BfmI CTRYAG 2 cut(s) 105, 409
BfuI GTATCC 1 cut(s) 123
BisI GCNGC 1 cut(s) 679
BlnI CCTAGG 1 cut(s) 398
BlsI GCNGC 1 cut(s) 680
BmcAI AGTACT 1 cut(s) 19
Bme1390I CCNGG 4 cut(s) 256, 539, 656, 675
Bme18I GGWCC 3 cut(s) 176, 604, 691
BmeRI GACNNNNNGTC 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 599
BmgT120I GGNCC 4 cut(s) 176, 541, 604, 691
BmiI GGNNCC 2 cut(s) 660, 756
BmrFI CCNGG 4 cut(s) 256, 539, 656, 675
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 2 cut(s) 419, 679
BmuI ACTGGG 1 cut(s) 228
BpmI CTGGAG 1 cut(s) 633
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 3 cut(s) 301, 609, 687
BpuMI CCSGG 2 cut(s) 656, 675
BsaAI YACGTR 1 cut(s) 684
BsaBI GATNNNNATC 1 cut(s) 669
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 398, 537, 600
BsaWI WCCGGW 1 cut(s) 150
Bsc4I CCNNNNNNNGG 2 cut(s) 538, 601
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 2 cut(s) 223, 334
Bse3DI GCAATG 1 cut(s) 18
Bse8I GATNNNNATC 1 cut(s) 669
BseBI CCWGG 2 cut(s) 256, 539
BseDI CCNNGG 3 cut(s) 398, 537, 600
BseGI GGATG 1 cut(s) 670
BseJI GATNNNNATC 1 cut(s) 669
BseLI CCNNNNNNNGG 2 cut(s) 538, 601
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 223, 334
BseRI GAGGAG 2 cut(s) 59, 113
BseSI GKGCMC 1 cut(s) 419
BsgI GTGCAG 1 cut(s) 179
Bsh1236I CGCG 1 cut(s) 637
BshFI GGCC 3 cut(s) 498, 542, 850
BshNI GGYRCC 1 cut(s) 754
BshTI ACCGGT 1 cut(s) 150
BsiHKAI GWGCWC 2 cut(s) 297, 419
BsiHKCI CYCGRG 1 cut(s) 599
BsiSI CCGG 3 cut(s) 151, 656, 675
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 2 cut(s) 538, 601
BsmAI GTCTC 2 cut(s) 81, 191
BsmFI GGGAC 1 cut(s) 156
BsnI GGCC 3 cut(s) 498, 542, 850
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 599
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 297, 419
Bsp143I GATC 4 cut(s) 312, 466, 664, 870
BspACI CCGC 4 cut(s) 116, 201, 678, 689
BspANI GGCC 3 cut(s) 498, 542, 850
BspFNI CGCG 1 cut(s) 637
BspLI GGNNCC 2 cut(s) 660, 756
BspPI GGATC 2 cut(s) 461, 878
BspT104I TTCGAA 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 754
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 150
BsrI ACTGG 2 cut(s) 223, 334
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 3 cut(s) 398, 537, 600
BssMI GATC 4 cut(s) 312, 466, 664, 870
BssNAI GTATAC 1 cut(s) 303
BssSI CACGAG 1 cut(s) 611
BssT1I CCWWGG 1 cut(s) 398
Bst1107I GTATAC 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 611
Bst2UI CCWGG 2 cut(s) 256, 539
Bst4CI ACNGT 3 cut(s) 31, 229, 815
Bst6I CTCTTC 2 cut(s) 36, 714
BstBAI YACGTR 1 cut(s) 684
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 635
BstENI CCTNNNNNAGG 1 cut(s) 536
BstF5I GGATG 1 cut(s) 670
BstFNI CGCG 1 cut(s) 637
BstKTI GATC 4 cut(s) 315, 469, 667, 873
BstMAI GTCTC 2 cut(s) 81, 191
BstMBI GATC 4 cut(s) 312, 466, 664, 870
BstMWI GCNNNNNNNGC 1 cut(s) 639
BstNI CCWGG 2 cut(s) 256, 539
BstSCI CCNGG 4 cut(s) 254, 537, 654, 673
BstSFI CTRYAG 2 cut(s) 105, 409
BstSLI GKGCMC 1 cut(s) 419
BstUI CGCG 1 cut(s) 637
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BstZ17I GTATAC 1 cut(s) 303
BsuI GTATCC 1 cut(s) 123
BsuRI GGCC 3 cut(s) 498, 542, 850
BtsCI GGATG 1 cut(s) 670
BtsI GCAGTG 1 cut(s) 439
BtsIMutI CAGTG 2 cut(s) 327, 439
Cac8I GCNNGC 1 cut(s) 635
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 4 cut(s) 176, 541, 604, 691
Csp6I GTAC 1 cut(s) 18
CspAI ACCGGT 1 cut(s) 150
CviAII CATG 2 cut(s) 188, 846
CviQI GTAC 1 cut(s) 18
DpnI GATC 4 cut(s) 314, 468, 666, 872
DpnII GATC 4 cut(s) 312, 466, 664, 870
DrdI GACNNNNNNGTC 1 cut(s) 205
DriI GACNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 1 cut(s) 205
Eam1104I CTCTTC 2 cut(s) 36, 714
Eam1105I GACNNNNNGTC 1 cut(s) 73
EarI CTCTTC 2 cut(s) 36, 714
Eco130I CCWWGG 1 cut(s) 398
Eco147I AGGCCT 1 cut(s) 498
Eco31I GGTCTC 1 cut(s) 191
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 3 cut(s) 176, 604, 691
Eco57I CTGAAG 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 599
EcoNI CCTNNNNNAGG 1 cut(s) 536
EcoRII CCWGG 2 cut(s) 254, 537
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 398
ErhI CCWWGG 1 cut(s) 398
FaeI CATG 2 cut(s) 191, 849
FalI AAGNNNNNCTT 8 cut(s) 33, 65, 243, 275, 726, 758, 772, 804
FaqI GGGAC 1 cut(s) 156
FatI CATG 2 cut(s) 187, 845
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 1 cut(s) 679
FokI GGATG 1 cut(s) 657
Fsp4HI GCNGC 1 cut(s) 679
FspBI CTAG 2 cut(s) 399, 852
GluI GCNGC 1 cut(s) 679
GsuI CTGGAG 1 cut(s) 633
HaeIII GGCC 3 cut(s) 498, 542, 850
HapII CCGG 3 cut(s) 151, 656, 675
Hin1II CATG 2 cut(s) 191, 849
HinfI GANTC 2 cut(s) 74, 553
HpaII CCGG 3 cut(s) 151, 656, 675
HphI GGTGA 3 cut(s) 478, 579, 810
Hpy166II GTNNAC 3 cut(s) 303, 417, 818
Hpy188I TCNGA 1 cut(s) 309
Hpy188III TCNNGA 8 cut(s) 65, 174, 196, 382, 470, 626, 650, 704
Hpy8I GTNNAC 3 cut(s) 303, 417, 818
HpyAV CCTTC 3 cut(s) 488, 786, 843
HpyCH4III ACNGT 3 cut(s) 31, 229, 815
HpyCH4IV ACGT 2 cut(s) 165, 683
HpyCH4V TGCA 6 cut(s) 160, 417, 432, 437, 515, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 639
HpySE526I ACGT 2 cut(s) 165, 683
Hsp92II CATG 2 cut(s) 191, 849
Kzo9I GATC 4 cut(s) 312, 466, 664, 870
LmnI GCTCC 3 cut(s) 46, 58, 402
LweI GCATC 2 cut(s) 419, 679
MaeI CTAG 2 cut(s) 399, 852
MaeII ACGT 2 cut(s) 165, 683
MaeIII GTNAC 5 cut(s) 90, 211, 323, 643, 729
MalI GATC 4 cut(s) 314, 468, 666, 872
MboI GATC 4 cut(s) 312, 466, 664, 870
MboII GAAGA 9 cut(s) 53, 322, 476, 589, 728, 731, 758, 780, 789
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 2 cut(s) 297, 419
MluCI AATT 2 cut(s) 22, 793
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 2 cut(s) 256, 848
MslI CAYNNNNRTG 1 cut(s) 332
MspI CCGG 3 cut(s) 151, 656, 675
MspR9I CCNGG 4 cut(s) 256, 539, 656, 675
MvaI CCWGG 2 cut(s) 256, 539
MvnI CGCG 1 cut(s) 637
MwoI GCNNNNNNNGC 1 cut(s) 639
NciI CCSGG 2 cut(s) 656, 675
NdeII GATC 4 cut(s) 312, 466, 664, 870
NlaIII CATG 2 cut(s) 191, 849
NlaIV GGNNCC 2 cut(s) 660, 756
NmuCI GTSAC 3 cut(s) 211, 323, 729
NspV TTCGAA 1 cut(s) 39
PceI AGGCCT 1 cut(s) 498
PfeI GAWTC 1 cut(s) 553
PinAI ACCGGT 1 cut(s) 150
PkrI GCNGC 1 cut(s) 680
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
Ppu21I YACGTR 1 cut(s) 684
Psp6I CCWGG 2 cut(s) 254, 537
PspGI CCWGG 2 cut(s) 254, 537
PspN4I GGNNCC 2 cut(s) 660, 756
PspPI GGNCC 4 cut(s) 176, 541, 604, 691
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 1 cut(s) 19
RsaNI GTAC 1 cut(s) 18
RseI CAYNNNNRTG 1 cut(s) 332
SatI GCNGC 1 cut(s) 679
Sau3AI GATC 4 cut(s) 312, 466, 664, 870
Sau96I GGNCC 4 cut(s) 176, 541, 604, 691
ScaI AGTACT 1 cut(s) 19
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 4 cut(s) 256, 539, 656, 675
SduI GDGCHC 2 cut(s) 297, 419
SfaNI GCATC 2 cut(s) 419, 679
SfcI CTRYAG 2 cut(s) 105, 409
SfuI TTCGAA 1 cut(s) 39
SinI GGWCC 3 cut(s) 176, 604, 691
SmiMI CAYNNNNRTG 1 cut(s) 332
SmlI CTYRAG 3 cut(s) 280, 624, 702
SmoI CTYRAG 3 cut(s) 280, 624, 702
Sse9I AATT 2 cut(s) 22, 793
SseBI AGGCCT 1 cut(s) 498
SsiI CCGC 4 cut(s) 116, 201, 678, 689
SspI AATATT 1 cut(s) 267
SspMI CTAG 2 cut(s) 399, 852
StuI AGGCCT 1 cut(s) 498
StyD4I CCNGG 4 cut(s) 254, 537, 654, 673
StyI CCWWGG 1 cut(s) 398
TaaI ACNGT 3 cut(s) 31, 229, 815
TaiI ACGT 2 cut(s) 168, 686
TaqI TCGA 2 cut(s) 39, 195
TasI AATT 2 cut(s) 22, 793
TatI WGTACW 1 cut(s) 17
TauI GCSGC 1 cut(s) 681
TfiI GAWTC 1 cut(s) 553
TscAI CASTG 2 cut(s) 334, 439
TseFI GTSAC 3 cut(s) 211, 323, 729
Tsp45I GTSAC 3 cut(s) 211, 323, 729
TspDTI ATGAA 3 cut(s) 507, 825, 834
TspRI CASTG 2 cut(s) 334, 439
VneI GTGCAC 1 cut(s) 415
VpaK11BI GGWCC 3 cut(s) 176, 604, 691
XagI CCTNNNNNAGG 1 cut(s) 536
XapI RAATTY 1 cut(s) 793
XmaJI CCTAGG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 302
XspI CTAG 2 cut(s) 399, 852
ZrmI AGTACT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.