Rorug03G0081100
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0015149
Physical Location & Seq
Reverse (-)
6336651 .. 6337442
792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0081100.1

Sequence Viewer

Length: 396 bp
ATGGAAGGAGAAAGAAAGAAAAGAAAGATGGAATATGAAGAAGAGGATGATGATGAGGAAAAGATTGAGAAATTCTTCGCTCTGATTAAGAGTACGAGACAGGTGCGTCACCTACTCGGGGGAAGCTCGAAATGGACCAAGGAGAAGAGAGATCAGAATAAGAAGGAAGAGGATCAAGAGAAGGCTACCGCCGGCAGTGCTTGGAACCCAGCGTTCCAACCAGAAGACTTCCTTGATCAAGATGATAAGAGTGCTAGTGGTGATCATGATGCAAGCCCTTTTAAAATTCAAGATGATAAGAGTACTGGTGCTGATCATGGTGCAAGCCCTTGTAAAATTCAAGAAGAAATGGAAGAAGATGGAGGTAATGGTTTAGACCTGAAGCTTTCTCTGTAG

Protein Analysis

131

Amino Acids

14.89

Weight (kDa)

4.72

Isoelectric Point (pI)

53.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPR1_interact PF15699 14 - 128 1.1e-19 NPR1 interacting
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43290
fragaria_vesca FvH4_6g13470
malus_domestica MD04G1131000.v1.1 MD12G1144100.v1.1
prunus_persica Prupe.6G257500_v2.0.a1
pyrus_communis pycom04g11930 pycom12g13870
rosa_chinensis RchiOBHm_Chr3g0466341
rosa_laevigata RLG00000024584
rosa_multiflora Rmu_sc0003480.1_g000013
rosa_roxburghii Rroxscaffold_6G00414560
rosa_rugosa Rorug03G0081100
rosa_samantha Rh3AG127400 Rh3BG148800 Rh3DG149500
rosa_wichuraiana Rw3G012040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 105
AciI CCGC 1 cut(s) 189
AclWI GGATC 1 cut(s) 180
AcsI RAATTY 3 cut(s) 71, 285, 336
AfaI GTAC 2 cut(s) 94, 304
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 2 cut(s) 290, 341
AluBI AGCT 2 cut(s) 126, 385
AluI AGCT 2 cut(s) 126, 385
Alw26I GTCTC 1 cut(s) 91
AlwI GGATC 1 cut(s) 180
Ama87I CYCGRG 1 cut(s) 116
ApoI RAATTY 3 cut(s) 71, 285, 336
Asp700I GAANNNNTTC 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 135
AsuHPI GGTGA 2 cut(s) 101, 272
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 231
BccI CCATC 2 cut(s) 22, 353
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BclI TGATCA 3 cut(s) 235, 262, 313
BcoDI GTCTC 1 cut(s) 91
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 1 cut(s) 392
BmcAI AGTACT 1 cut(s) 304
Bme18I GGWCC 1 cut(s) 135
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 135
BmiI GGNNCC 1 cut(s) 206
BmsI GCATC 1 cut(s) 259
BpiI GAAGAC 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 138
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 1 cut(s) 310
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 1 cut(s) 52
BseLI CCNNNNNNNGG 1 cut(s) 118
BseNI ACTGG 1 cut(s) 310
BseYI CCCAGC 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 1 cut(s) 192
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 1 cut(s) 91
BsoBI CYCGRG 1 cut(s) 116
Bsp143I GATC 5 cut(s) 151, 172, 235, 262, 313
BspACI CCGC 1 cut(s) 189
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 206
BspPI GGATC 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 1 cut(s) 310
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 5 cut(s) 151, 172, 235, 262, 313
BssT1I CCWWGG 1 cut(s) 138
Bst6I CTCTTC 3 cut(s) 36, 140, 162
BstC8I GCNNGC 3 cut(s) 193, 274, 325
BstF5I GGATG 1 cut(s) 52
BstKTI GATC 5 cut(s) 154, 175, 238, 265, 316
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 5 cut(s) 151, 172, 235, 262, 313
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstSFI CTRYAG 1 cut(s) 392
BstV2I GAAGAC 1 cut(s) 231
BtsCI GGATG 1 cut(s) 52
BtsI GCAGTG 1 cut(s) 202
BtsIMutI CAGTG 1 cut(s) 202
Cac8I GCNNGC 3 cut(s) 193, 274, 325
CciI TCATGA 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 1 cut(s) 135
CseI GACGC 1 cut(s) 95
Csp6I GTAC 2 cut(s) 93, 303
CviAII CATG 2 cut(s) 266, 317
CviJI RGCY 5 cut(s) 126, 185, 276, 327, 385
CviKI_1 RGCY 5 cut(s) 126, 185, 276, 327, 385
CviQI GTAC 2 cut(s) 93, 303
DpnI GATC 5 cut(s) 153, 174, 237, 264, 315
DpnII GATC 5 cut(s) 151, 172, 235, 262, 313
DraI TTTAAA 1 cut(s) 283
DrdI GACNNNNNNGTC 1 cut(s) 105
DseDI GACNNNNNNGTC 1 cut(s) 105
Eam1104I CTCTTC 3 cut(s) 36, 140, 162
EarI CTCTTC 3 cut(s) 36, 140, 162
Eco130I CCWWGG 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 135
Eco88I CYCGRG 1 cut(s) 116
EcoT14I CCWWGG 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 2 cut(s) 269, 320
FaiI YATR 3 cut(s) 36, 267, 318
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FatI CATG 2 cut(s) 265, 316
FbaI TGATCA 3 cut(s) 235, 262, 313
FokI GGATG 1 cut(s) 59
FspBI CTAG 1 cut(s) 255
GsaI CCCAGC 1 cut(s) 212
HapII CCGG 1 cut(s) 192
HgaI GACGC 1 cut(s) 95
Hin1II CATG 2 cut(s) 269, 320
HindIII AAGCTT 1 cut(s) 383
HpaII CCGG 1 cut(s) 192
HphI GGTGA 2 cut(s) 101, 272
Hpy188I TCNGA 2 cut(s) 84, 156
Hpy188III TCNNGA 5 cut(s) 176, 239, 266, 290, 341
HpyAV CCTTC 2 cut(s) 157, 175
HpyCH4V TGCA 2 cut(s) 272, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
Hsp92II CATG 2 cut(s) 269, 320
KroI GCCGGC 1 cut(s) 191
KroNI GCCGGC 1 cut(s) 193
Ksp22I TGATCA 3 cut(s) 235, 262, 313
Kzo9I GATC 5 cut(s) 151, 172, 235, 262, 313
LpnPI CCDG 6 cut(s) 86, 205, 222, 234, 291, 392
LweI GCATC 1 cut(s) 259
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 107
MalI GATC 5 cut(s) 153, 174, 237, 264, 315
MboI GATC 5 cut(s) 151, 172, 235, 262, 313
MboII GAAGA 9 cut(s) 50, 53, 67, 157, 179, 236, 356, 365, 368
MluCI AATT 3 cut(s) 71, 285, 336
MmeI TCCRAC 1 cut(s) 241
MnlI CCTC 4 cut(s) 37, 49, 163, 356
MroNI GCCGGC 1 cut(s) 191
MroXI GAANNNNTTC 1 cut(s) 74
MseI TTAA 2 cut(s) 87, 282
MspI CCGG 1 cut(s) 192
MwoI GCNNNNNNNGC 1 cut(s) 197
NaeI GCCGGC 1 cut(s) 193
NdeII GATC 5 cut(s) 151, 172, 235, 262, 313
NgoMIV GCCGGC 1 cut(s) 191
NlaIII CATG 2 cut(s) 269, 320
NlaIV GGNNCC 1 cut(s) 206
NmuCI GTSAC 1 cut(s) 107
PagI TCATGA 1 cut(s) 265
PdiI GCCGGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 74
PspFI CCCAGC 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 206
PspPI GGNCC 1 cut(s) 135
RsaI GTAC 2 cut(s) 94, 304
RsaNI GTAC 2 cut(s) 93, 303
SaqAI TTAA 2 cut(s) 87, 282
Sau3AI GATC 5 cut(s) 151, 172, 235, 262, 313
Sau96I GGNCC 1 cut(s) 135
ScaI AGTACT 1 cut(s) 304
SetI ASST 6 cut(s) 105, 114, 128, 367, 381, 387
SfaNI GCATC 1 cut(s) 259
SfcI CTRYAG 1 cut(s) 392
SinI GGWCC 1 cut(s) 135
Sse9I AATT 3 cut(s) 71, 285, 336
SsiI CCGC 1 cut(s) 189
SspMI CTAG 1 cut(s) 255
StyI CCWWGG 1 cut(s) 138
TaqI TCGA 1 cut(s) 128
TasI AATT 3 cut(s) 71, 285, 336
TatI WGTACW 1 cut(s) 302
Tru1I TTAA 2 cut(s) 87, 282
Tru9I TTAA 2 cut(s) 87, 282
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 107
TspDTI ATGAA 1 cut(s) 51
TspRI CASTG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 135
XapI RAATTY 3 cut(s) 71, 285, 336
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 1 cut(s) 255
ZrmI AGTACT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.