AT5G43460

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011782
Physical Location & Seq
Forward (+)
17459873 .. 17462516
2644 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G43460.2

Sequence Viewer

Length: 465 bp
ATGGGTTTCTTCTCTTTTCTTGGAAGAGTTCTCTTCGCTTCTCTCTTTATCCTCTCCGCTTGGCAAATGTTCAATGATGGTGGTCCTGCAGCTAAAGAATTGGCTCCAAAGCTTGATTTGGTTAAGGCACATTTATCTTCTAGATTAGGAGTAACATTGCCCAAAATAGAGGTGAGACAAGTCGTTGCTACTATTATTGCCCTGAAAGGAGTCGGAGGCTTACTCTTTGTTATCGGAAACACCTTCGGTGCTTATCTTCTGGCTTCCTACTTGGTGGTTTTCGGTCCTATTCTCTATGATTTCTACAACTACGGACCTCAGGAACGTCATTTCTCTCTTCTGTTGACAGAGTTCTTGCAGAGCGTTGCACTCTTTGGAGCGTTACTCTTCTTTATCGGAATGAAGAACTCGACACCCGCCAAGAAGAATTTGAAGAAGAGGACACCTAAGCCGAAAGCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

154

Amino Acids

16.9

Weight (kDa)

10.05

Isoelectric Point (pI)

32.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 134 8.4e-54 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011782)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04340 AT5G43460 AT5G43460
fragaria_vesca FvH4_4g03211 FvH4_5g30450
malus_domestica MD13G1210700.v1.1 MD16G1213900.v1.1
prunus_persica Prupe.1G035100_v2.0.a1
pyrus_communis pycom13g18320 pycom16g17970
rosa_chinensis RchiOBHm_Chr4g0391891
rosa_laevigata RLG00000009851
rosa_multiflora Rmu_co8463711.1_g000001 Rmu_sc0000383.1_g000013 Rmu_sc0007693.1_g000011
rosa_rugosa Rorug03G0336700
rosa_samantha Rh4AG039100 Rh4BG033800
rosa_wichuraiana Rw4G003050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 57, 417
AcsI RAATTY 1 cut(s) 427
AgsI TTSAA 2 cut(s) 73, 433
AluBI AGCT 3 cut(s) 92, 112, 458
AluI AGCT 3 cut(s) 92, 112, 458
Alw26I GTCTC 1 cut(s) 169
ApeKI GCWGC 2 cut(s) 89, 458
ApoI RAATTY 1 cut(s) 427
AspS9I GGNCC 3 cut(s) 83, 284, 314
AsuHPI GGTGA 1 cut(s) 184
AvaII GGWCC 3 cut(s) 83, 284, 314
AxyI CCTNAGG 1 cut(s) 318
BbvI GCAGC 2 cut(s) 101, 445
BccI CCATC 1 cut(s) 71
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 2 cut(s) 141, 463
BfmI CTRYAG 1 cut(s) 87
BisI GCNGC 2 cut(s) 90, 459
BlsI GCNGC 2 cut(s) 91, 460
Bme18I GGWCC 3 cut(s) 83, 284, 314
BmgT120I GGNCC 3 cut(s) 83, 284, 314
BmiI GGNNCC 1 cut(s) 105
BplI GAGNNNNNCTC 4 cut(s) 207, 239, 369, 401
Bpu10I CCTNAGC 1 cut(s) 447
Bse21I CCTNAGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 155
BseMI GCAATG 1 cut(s) 155
BseMII CTCAG 1 cut(s) 332
BseXI GCAGC 2 cut(s) 101, 445
BsmAI GTCTC 1 cut(s) 169
BspACI CCGC 2 cut(s) 57, 417
BspCNI CTCAG 1 cut(s) 331
BspLI GGNNCC 1 cut(s) 105
BspMAI CTGCAG 1 cut(s) 91
BsrDI GCAATG 1 cut(s) 155
Bst6I CTCTTC 5 cut(s) 19, 38, 342, 392, 431
BstDEI CTNAG 2 cut(s) 318, 447
BstMAI GTCTC 1 cut(s) 169
BstSFI CTRYAG 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 101, 445
Bsu36I CCTNAGG 1 cut(s) 318
Cfr13I GGNCC 3 cut(s) 83, 284, 314
CspCI CAANNNNNGTGG 2 cut(s) 61, 96
CviJI RGCY 7 cut(s) 92, 104, 112, 219, 263, 451, 458
CviKI_1 RGCY 7 cut(s) 92, 104, 112, 219, 263, 451, 458
DdeI CTNAG 2 cut(s) 318, 447
Eam1104I CTCTTC 5 cut(s) 19, 38, 342, 392, 431
EarI CTCTTC 5 cut(s) 19, 38, 342, 392, 431
Eco47I GGWCC 3 cut(s) 83, 284, 314
Eco81I CCTNAGG 1 cut(s) 318
FaiI YATR 1 cut(s) 297
FauI CCCGC 1 cut(s) 424
Fnu4HI GCNGC 2 cut(s) 90, 459
Fsp4HI GCNGC 2 cut(s) 90, 459
FspBI CTAG 2 cut(s) 141, 463
GluI GCNGC 2 cut(s) 90, 459
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 1 cut(s) 210
HphI GGTGA 1 cut(s) 184
Hpy166II GTNNAC 1 cut(s) 345
Hpy188I TCNGA 3 cut(s) 215, 236, 398
Hpy188III TCNNGA 2 cut(s) 141, 320
Hpy8I GTNNAC 1 cut(s) 345
HpyAV CCTTC 1 cut(s) 253
HpyCH4IV ACGT 1 cut(s) 325
HpyCH4V TGCA 3 cut(s) 89, 358, 368
HpyF3I CTNAG 2 cut(s) 318, 447
HpySE526I ACGT 1 cut(s) 325
LmnI GCTCC 2 cut(s) 109, 377
LpnPI CCDG 4 cut(s) 99, 215, 245, 305
Lsp1109I GCAGC 2 cut(s) 101, 445
MaeI CTAG 2 cut(s) 141, 463
MaeII ACGT 1 cut(s) 325
MaeIII GTNAC 2 cut(s) 151, 381
MluCI AATT 2 cut(s) 98, 427
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 5 cut(s) 62, 163, 209, 327, 432
MseI TTAA 1 cut(s) 123
NlaIV GGNNCC 1 cut(s) 105
PkrI GCNGC 2 cut(s) 91, 460
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PspN4I GGNNCC 1 cut(s) 105
PspPI GGNCC 3 cut(s) 83, 284, 314
PstI CTGCAG 1 cut(s) 91
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 2 cut(s) 90, 459
Sau96I GGNCC 3 cut(s) 83, 284, 314
SchI GAGTC 1 cut(s) 219
SetI ASST 8 cut(s) 94, 114, 174, 245, 319, 328, 448, 460
SfcI CTRYAG 1 cut(s) 87
SinI GGWCC 3 cut(s) 83, 284, 314
Sse9I AATT 2 cut(s) 98, 427
SsiI CCGC 2 cut(s) 57, 417
SspMI CTAG 2 cut(s) 141, 463
TaiI ACGT 1 cut(s) 328
TaqI TCGA 1 cut(s) 410
TaqII GACCGA 1 cut(s) 272
TasI AATT 2 cut(s) 98, 427
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TseI GCWGC 2 cut(s) 89, 458
TspDTI ATGAA 1 cut(s) 416
TspGWI ACGGA 1 cut(s) 327
VpaK11BI GGWCC 3 cut(s) 83, 284, 314
XapI RAATTY 1 cut(s) 427
XbaI TCTAGA 1 cut(s) 140
XspI CTAG 2 cut(s) 141, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.