RLG00000009851

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011782
Physical Location & Seq
Forward (+)
58906797 .. 58909003
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009851

Sequence Viewer

Length: 396 bp
ATGGGGTTCTTCTCCTTCTTCGGCCGCCTTCGTGCATATATCATTCTGTCATGTTGCTCATGTGTAGTATGGCTTTTGATGTGGGAATATTGTGTGCAGCCTATTCATTTAGCTGGAGCTGTGATAGCTCTAAAAGGTCTGGGAGGCATTCTATTTGTACTGAACAGTAATATTGGAGCTCTTCTCCTGATTATTCAACTGGCATTTACCACTCCCCTCATCTATGATTTCTACAACTTTAGTCCAGACACTCCCAAATTTGGTGTGCTCCTGAATGATTTCTTGCAGCAAGCAGCACTTGTTGGTGCACTGTTTTTCTTCATCGGGATGAAGAACTCTATTTCTAAGAGGGGACAAATCACCAAGAAGAAGATCCACAAAACCAAAACTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

132

Amino Acids

14.59

Weight (kDa)

9.61

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 36 - 110 6.3e-24 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011782)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04340 AT5G43460 AT5G43460
fragaria_vesca FvH4_4g03211 FvH4_5g30450
malus_domestica MD13G1210700.v1.1 MD16G1213900.v1.1
prunus_persica Prupe.1G035100_v2.0.a1
pyrus_communis pycom13g18320 pycom16g17970
rosa_chinensis RchiOBHm_Chr4g0391891
rosa_laevigata RLG00000009851
rosa_multiflora Rmu_co8463711.1_g000001 Rmu_sc0000383.1_g000013 Rmu_sc0007693.1_g000011
rosa_rugosa Rorug03G0336700
rosa_samantha Rh4AG039100 Rh4BG033800
rosa_wichuraiana Rw4G003050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 25
AclWI GGATC 1 cut(s) 367
AcoI YGGCCR 1 cut(s) 22
AcsI RAATTY 1 cut(s) 257
AfaI GTAC 1 cut(s) 159
AfiI CCNNNNNNNGG 1 cut(s) 260
AgsI TTSAA 1 cut(s) 197
AluBI AGCT 4 cut(s) 113, 119, 128, 179
AluI AGCT 4 cut(s) 113, 119, 128, 179
Alw21I GWGCWC 3 cut(s) 181, 270, 310
Alw44I GTGCAC 1 cut(s) 306
AlwI GGATC 1 cut(s) 367
AoxI GGCC 1 cut(s) 22
ApaLI GTGCAC 1 cut(s) 306
ApeKI GCWGC 3 cut(s) 97, 286, 293
ApoI RAATTY 1 cut(s) 257
Asp700I GAANNNNTTC 1 cut(s) 278
AsuHPI GGTGA 1 cut(s) 352
BaeGI GKGCMC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 181
Bbv12I GWGCWC 3 cut(s) 181, 270, 310
BbvI GCAGC 3 cut(s) 109, 298, 305
BisI GCNGC 4 cut(s) 25, 98, 287, 294
BlsI GCNGC 4 cut(s) 26, 99, 288, 295
BplI GAGNNNNNCTC 2 cut(s) 168, 200
BpmI CTGGAG 1 cut(s) 135
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 204, 394
BseGI GGATG 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 260
BseNI ACTGG 2 cut(s) 204, 394
BseSI GKGCMC 1 cut(s) 310
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 3 cut(s) 109, 298, 305
BsgI GTGCAG 1 cut(s) 116
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 1 cut(s) 24
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 3 cut(s) 181, 270, 310
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 366
BsmI GAATGC 1 cut(s) 147
BsnI GGCC 1 cut(s) 24
Bsp1286I GDGCHC 3 cut(s) 181, 270, 310
Bsp143I GATC 1 cut(s) 372
BspACI CCGC 1 cut(s) 25
BspANI GGCC 1 cut(s) 24
BspPI GGATC 1 cut(s) 367
BspQI GCTCTTC 1 cut(s) 186
BsrI ACTGG 2 cut(s) 204, 394
BssMI GATC 1 cut(s) 372
Bst4CI ACNGT 2 cut(s) 167, 312
Bst6I CTCTTC 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 1 cut(s) 345
BstF5I GGATG 1 cut(s) 333
BstKTI GATC 1 cut(s) 375
BstMBI GATC 1 cut(s) 372
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstSLI GKGCMC 1 cut(s) 310
BstV1I GCAGC 3 cut(s) 109, 298, 305
BstX2I RGATCY 1 cut(s) 372
BstYI RGATCY 1 cut(s) 372
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 1 cut(s) 24
BtsCI GGATG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 291
Csp6I GTAC 1 cut(s) 158
CviAII CATG 2 cut(s) 51, 60
CviJI RGCY 7 cut(s) 24, 73, 100, 113, 119, 128, 179
CviKI_1 RGCY 7 cut(s) 24, 73, 100, 113, 119, 128, 179
CviQI GTAC 1 cut(s) 158
DdeI CTNAG 1 cut(s) 345
DpnI GATC 1 cut(s) 374
DpnII GATC 1 cut(s) 372
EaeI YGGCCR 1 cut(s) 22
EagI CGGCCG 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 179
EclXI CGGCCG 1 cut(s) 22
Eco24I GRGCYC 1 cut(s) 181
Eco52I CGGCCG 1 cut(s) 22
Eco53kI GAGCTC 1 cut(s) 179
EcoICRI GAGCTC 1 cut(s) 179
EcoT38I GRGCYC 1 cut(s) 181
FaeI CATG 2 cut(s) 54, 63
FaiI YATR 6 cut(s) 37, 39, 52, 61, 70, 225
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FaqI GGGAC 1 cut(s) 366
FatI CATG 2 cut(s) 50, 59
Fnu4HI GCNGC 4 cut(s) 25, 98, 287, 294
FokI GGATG 1 cut(s) 340
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 4 cut(s) 25, 98, 287, 294
GluI GCNGC 4 cut(s) 25, 98, 287, 294
GsuI CTGGAG 1 cut(s) 135
HaeIII GGCC 1 cut(s) 24
Hin1II CATG 2 cut(s) 54, 63
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 1 cut(s) 308
Hpy188III TCNNGA 4 cut(s) 187, 245, 271, 325
Hpy8I GTNNAC 1 cut(s) 308
HpyAV CCTTC 2 cut(s) 25, 38
HpyCH4III ACNGT 2 cut(s) 167, 312
HpyCH4V TGCA 4 cut(s) 35, 97, 286, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 1 cut(s) 345
Hsp92II CATG 2 cut(s) 54, 63
Kzo9I GATC 1 cut(s) 372
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 3 cut(s) 116, 176, 273
LpnPI CCDG 7 cut(s) 99, 125, 185, 200, 258, 284, 375
Lsp1109I GCAGC 3 cut(s) 109, 298, 305
MalI GATC 1 cut(s) 374
MboI GATC 1 cut(s) 372
MboII GAAGA 6 cut(s) 10, 173, 310, 343, 379, 382
MflI RGATCY 1 cut(s) 372
MhlI GDGCHC 3 cut(s) 181, 270, 310
MluCI AATT 1 cut(s) 257
MnlI CCTC 3 cut(s) 137, 227, 342
MroXI GAANNNNTTC 1 cut(s) 278
MslI CAYNNNNRTG 1 cut(s) 326
Mva1269I GAATGC 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 125
NdeII GATC 1 cut(s) 372
NlaIII CATG 2 cut(s) 54, 63
PciSI GCTCTTC 1 cut(s) 186
PctI GAATGC 1 cut(s) 147
PdmI GAANNNNTTC 1 cut(s) 278
PkrI GCNGC 4 cut(s) 26, 99, 288, 295
Psp124BI GAGCTC 1 cut(s) 181
PsuI RGATCY 1 cut(s) 372
RsaI GTAC 1 cut(s) 159
RsaNI GTAC 1 cut(s) 158
RseI CAYNNNNRTG 1 cut(s) 326
SacI GAGCTC 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 186
SatI GCNGC 4 cut(s) 25, 98, 287, 294
Sau3AI GATC 1 cut(s) 372
SduI GDGCHC 3 cut(s) 181, 270, 310
SetI ASST 5 cut(s) 115, 121, 130, 139, 181
SmiMI CAYNNNNRTG 1 cut(s) 326
Sse9I AATT 1 cut(s) 257
SsiI CCGC 1 cut(s) 25
SspI AATATT 2 cut(s) 89, 172
SstI GAGCTC 1 cut(s) 181
TaaI ACNGT 2 cut(s) 167, 312
TasI AATT 1 cut(s) 257
TatI WGTACW 1 cut(s) 157
TauI GCSGC 1 cut(s) 27
TscAI CASTG 1 cut(s) 315
TseI GCWGC 3 cut(s) 97, 286, 293
TspDTI ATGAA 3 cut(s) 95, 310, 344
TspRI CASTG 1 cut(s) 315
VneI GTGCAC 1 cut(s) 306
XapI RAATTY 1 cut(s) 257
XmnI GAANNNNTTC 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.