Rmu_co8463711.1_g000001

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8463711.1
OG0011782
Physical Location & Seq
Forward (+)
1 .. 1126
1126 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8463711.1_g000001.1.cds

Sequence Viewer

Length: 412 bp
gttcaatgaatttggtactgatggaggacctgctgcaaaggagttggctcccaaattttccgttttaaaagatcacctgtcttcgaaactgggctttgcattaccggatgtagatcctattcatttagctggagctgtgatagctctaaaaggtctgggaggcattctatttgtacttaacagtaatattggagctcttctcctgattattcaattggcatttaccactcccctcatctatgatttctacaactttagtccagacactcccaaatttggtgtactcctgaatgatttcttgcagcaagcagcacttgttggtgcattgtttttcttcatcgggatgaagaactctatttctaagaggggccaaatcaccaagaagaagatccacaaaaccaaaactggttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

136

Amino Acids

14.64

Weight (kDa)

9.56

Isoelectric Point (pI)

25.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011782)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04340 AT5G43460 AT5G43460
fragaria_vesca FvH4_4g03211 FvH4_5g30450
malus_domestica MD13G1210700.v1.1 MD16G1213900.v1.1
prunus_persica Prupe.1G035100_v2.0.a1
pyrus_communis pycom13g18320 pycom16g17970
rosa_chinensis RchiOBHm_Chr4g0391891
rosa_laevigata RLG00000009851
rosa_multiflora Rmu_co8463711.1_g000001 Rmu_sc0000383.1_g000013 Rmu_sc0007693.1_g000011
rosa_rugosa Rorug03G0336700
rosa_samantha Rh4AG039100 Rh4BG033800
rosa_wichuraiana Rw4G003050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 38
AclWI GGATC 2 cut(s) 108, 383
AcsI RAATTY 3 cut(s) 9, 54, 273
AfaI GTAC 3 cut(s) 17, 175, 283
AfiI CCNNNNNNNGG 1 cut(s) 276
AgsI TTSAA 2 cut(s) 5, 213
AluBI AGCT 4 cut(s) 129, 135, 144, 195
AluI AGCT 4 cut(s) 129, 135, 144, 195
Alw21I GWGCWC 1 cut(s) 197
AlwI GGATC 2 cut(s) 108, 383
AoxI GGCC 1 cut(s) 368
ApeKI GCWGC 3 cut(s) 33, 302, 309
ApoI RAATTY 3 cut(s) 9, 54, 273
Asp700I GAANNNNTTC 1 cut(s) 294
AspS9I GGNCC 2 cut(s) 27, 368
AsuHPI GGTGA 2 cut(s) 66, 368
AsuII TTCGAA 1 cut(s) 84
AvaII GGWCC 1 cut(s) 27
BanII GRGCYC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 197
BbvI GCAGC 3 cut(s) 20, 314, 321
BccI CCATC 1 cut(s) 15
BfuAI ACCTGC 1 cut(s) 38
BisI GCNGC 3 cut(s) 34, 303, 310
BlsI GCNGC 3 cut(s) 35, 304, 311
Bme18I GGWCC 1 cut(s) 27
BmgT120I GGNCC 2 cut(s) 27, 368
BmiI GGNNCC 2 cut(s) 49, 369
BmrI ACTGGG 1 cut(s) 99
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 73
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BpmI CTGGAG 1 cut(s) 151
Bpu14I TTCGAA 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 112
BsaWI WCCGGW 1 cut(s) 104
Bsc4I CCNNNNNNNGG 1 cut(s) 276
Bse1I ACTGG 2 cut(s) 94, 410
Bse8I GATNNNNATC 1 cut(s) 112
BseGI GGATG 2 cut(s) 113, 349
BseJI GATNNNNATC 1 cut(s) 112
BseLI CCNNNNNNNGG 1 cut(s) 276
BseNI ACTGG 2 cut(s) 94, 410
BseXI GCAGC 3 cut(s) 20, 314, 321
BshFI GGCC 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 197
BsiSI CCGG 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 276
BsmI GAATGC 1 cut(s) 163
BsnI GGCC 1 cut(s) 370
Bsp119I TTCGAA 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 197
Bsp143I GATC 3 cut(s) 71, 113, 388
BspANI GGCC 1 cut(s) 370
BspLI GGNNCC 2 cut(s) 49, 369
BspMI ACCTGC 1 cut(s) 38
BspPI GGATC 2 cut(s) 108, 383
BspQI GCTCTTC 1 cut(s) 202
BspT104I TTCGAA 1 cut(s) 84
BsrI ACTGG 2 cut(s) 94, 410
BssMI GATC 3 cut(s) 71, 113, 388
Bst4CI ACNGT 1 cut(s) 183
Bst6I CTCTTC 1 cut(s) 202
BstBI TTCGAA 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 307
BstDEI CTNAG 1 cut(s) 361
BstF5I GGATG 2 cut(s) 113, 349
BstKTI GATC 3 cut(s) 74, 116, 391
BstMBI GATC 3 cut(s) 71, 113, 388
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstV1I GCAGC 3 cut(s) 20, 314, 321
BstV2I GAAGAC 1 cut(s) 73
BstX2I RGATCY 2 cut(s) 113, 388
BstYI RGATCY 2 cut(s) 113, 388
BsuRI GGCC 1 cut(s) 370
BtsCI GGATG 2 cut(s) 113, 349
BveI ACCTGC 1 cut(s) 38
Cac8I GCNNGC 1 cut(s) 307
Cfr13I GGNCC 2 cut(s) 27, 368
Csp6I GTAC 3 cut(s) 16, 174, 282
CviJI RGCY 7 cut(s) 48, 94, 129, 135, 144, 195, 370
CviKI_1 RGCY 7 cut(s) 48, 94, 129, 135, 144, 195, 370
CviQI GTAC 3 cut(s) 16, 174, 282
DdeI CTNAG 1 cut(s) 361
DpnI GATC 3 cut(s) 73, 115, 390
DpnII GATC 3 cut(s) 71, 113, 388
DraI TTTAAA 1 cut(s) 67
Eam1104I CTCTTC 1 cut(s) 202
EarI CTCTTC 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 197
Eco47I GGWCC 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 195
EcoICRI GAGCTC 1 cut(s) 195
EcoO109I RGGNCCY 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 197
FaiI YATR 1 cut(s) 241
FalI AAGNNNNNCTT 2 cut(s) 298, 330
Fnu4HI GCNGC 3 cut(s) 34, 303, 310
FokI GGATG 2 cut(s) 120, 356
FriOI GRGCYC 1 cut(s) 197
Fsp4HI GCNGC 3 cut(s) 34, 303, 310
GluI GCNGC 3 cut(s) 34, 303, 310
GsuI CTGGAG 1 cut(s) 151
HaeIII GGCC 1 cut(s) 370
HapII CCGG 1 cut(s) 105
HpaII CCGG 1 cut(s) 105
HphI GGTGA 2 cut(s) 66, 368
Hpy166II GTNNAC 1 cut(s) 282
Hpy188III TCNNGA 4 cut(s) 203, 261, 287, 341
Hpy8I GTNNAC 1 cut(s) 282
HpyCH4III ACNGT 1 cut(s) 183
HpyCH4V TGCA 4 cut(s) 36, 99, 302, 324
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpyF3I CTNAG 1 cut(s) 361
Kzo9I GATC 3 cut(s) 71, 113, 388
LguI GCTCTTC 1 cut(s) 202
LmnI GCTCC 3 cut(s) 53, 132, 192
Lsp1109I GCAGC 3 cut(s) 20, 314, 321
MalI GATC 3 cut(s) 73, 115, 390
MboI GATC 3 cut(s) 71, 113, 388
MboII GAAGA 6 cut(s) 73, 189, 326, 359, 395, 398
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 2 cut(s) 113, 388
MhlI GDGCHC 1 cut(s) 197
MluCI AATT 4 cut(s) 9, 54, 213, 273
MnlI CCTC 4 cut(s) 18, 153, 243, 358
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 2 cut(s) 66, 178
MslI CAYNNNNRTG 1 cut(s) 342
MspI CCGG 1 cut(s) 105
MunI CAATTG 1 cut(s) 213
Mva1269I GAATGC 1 cut(s) 163
MwoI GCNNNNNNNGC 1 cut(s) 141
NdeII GATC 3 cut(s) 71, 113, 388
NlaIV GGNNCC 2 cut(s) 49, 369
NspV TTCGAA 1 cut(s) 84
PciSI GCTCTTC 1 cut(s) 202
PctI GAATGC 1 cut(s) 163
PdmI GAANNNNTTC 1 cut(s) 294
PkrI GCNGC 3 cut(s) 35, 304, 311
PpuMI RGGWCCY 1 cut(s) 27
Psp124BI GAGCTC 1 cut(s) 197
Psp5II RGGWCCY 1 cut(s) 27
PspN4I GGNNCC 2 cut(s) 49, 369
PspPI GGNCC 2 cut(s) 27, 368
PspPPI RGGWCCY 1 cut(s) 27
PsuI RGATCY 2 cut(s) 113, 388
RsaI GTAC 3 cut(s) 17, 175, 283
RsaNI GTAC 3 cut(s) 16, 174, 282
RseI CAYNNNNRTG 1 cut(s) 342
SacI GAGCTC 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 202
SaqAI TTAA 2 cut(s) 66, 178
SatI GCNGC 3 cut(s) 34, 303, 310
Sau3AI GATC 3 cut(s) 71, 113, 388
Sau96I GGNCC 2 cut(s) 27, 368
SduI GDGCHC 1 cut(s) 197
SetI ASST 7 cut(s) 32, 79, 131, 137, 146, 155, 197
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 1 cut(s) 27
SmiMI CAYNNNNRTG 1 cut(s) 342
Sse9I AATT 4 cut(s) 9, 54, 213, 273
SspI AATATT 1 cut(s) 188
SstI GAGCTC 1 cut(s) 197
TaaI ACNGT 1 cut(s) 183
TaqI TCGA 1 cut(s) 84
TasI AATT 4 cut(s) 9, 54, 213, 273
TatI WGTACW 2 cut(s) 173, 281
Tru1I TTAA 2 cut(s) 66, 178
Tru9I TTAA 2 cut(s) 66, 178
TseI GCWGC 3 cut(s) 33, 302, 309
TspDTI ATGAA 4 cut(s) 22, 111, 326, 360
TspGWI ACGGA 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 3 cut(s) 9, 54, 273
XmnI GAANNNNTTC 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.