Rh4AG039100

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011782
Physical Location & Seq
Reverse (-)
8220881 .. 8223704
2824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG039100.1

Sequence Viewer

Length: 318 bp
ATGTGGGAATATTGTGTGCAGCCTATTCATTTAGCTGGAGCTGTGATAGCTCTAAAAGGTCTGGGAGGCATTCTATTTGTACTTAACAGTAATATTGGAGCTCTTCTCCTGATTATTCAATTGGCATTTACCACTCCCCTCATCTATGATTTCTACAACTTTAGTCCAGACACTCCCAAATTTGGTGTACTCCTGAATGATTTCTTGCAGCAAGCAGCACTTGTTGGTGCACTGTTTTTCTTCATCGGGATGAAGAACTCTATTTCTAAGAGGGGCCAAATCACCAAGAAGAAGATCCACAAAACCAAAACTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

105

Amino Acids

11.57

Weight (kDa)

9.72

Isoelectric Point (pI)

27.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 10 - 84 2.5e-24 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011782)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04340 AT5G43460 AT5G43460
fragaria_vesca FvH4_4g03211 FvH4_5g30450
malus_domestica MD13G1210700.v1.1 MD16G1213900.v1.1
prunus_persica Prupe.1G035100_v2.0.a1
pyrus_communis pycom13g18320 pycom16g17970
rosa_chinensis RchiOBHm_Chr4g0391891
rosa_laevigata RLG00000009851
rosa_multiflora Rmu_co8463711.1_g000001 Rmu_sc0000383.1_g000013 Rmu_sc0007693.1_g000011
rosa_rugosa Rorug03G0336700
rosa_samantha Rh4AG039100 Rh4BG033800
rosa_wichuraiana Rw4G003050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 289
AcsI RAATTY 1 cut(s) 179
AfaI GTAC 2 cut(s) 81, 189
AfiI CCNNNNNNNGG 1 cut(s) 182
AgsI TTSAA 1 cut(s) 119
AluBI AGCT 4 cut(s) 35, 41, 50, 101
AluI AGCT 4 cut(s) 35, 41, 50, 101
Alw21I GWGCWC 2 cut(s) 103, 232
Alw44I GTGCAC 1 cut(s) 228
AlwI GGATC 1 cut(s) 289
AoxI GGCC 1 cut(s) 274
ApaLI GTGCAC 1 cut(s) 228
ApeKI GCWGC 3 cut(s) 19, 208, 215
ApoI RAATTY 1 cut(s) 179
Asp700I GAANNNNTTC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 274
AsuHPI GGTGA 1 cut(s) 274
BaeGI GKGCMC 1 cut(s) 232
BanII GRGCYC 1 cut(s) 103
Bbv12I GWGCWC 2 cut(s) 103, 232
BbvI GCAGC 3 cut(s) 31, 220, 227
BisI GCNGC 3 cut(s) 20, 209, 216
BlsI GCNGC 3 cut(s) 21, 210, 217
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 1 cut(s) 275
BplI GAGNNNNNCTC 2 cut(s) 90, 122
BpmI CTGGAG 1 cut(s) 57
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 316
BseGI GGATG 1 cut(s) 255
BseLI CCNNNNNNNGG 1 cut(s) 182
BseNI ACTGG 1 cut(s) 316
BseSI GKGCMC 1 cut(s) 232
BseXI GCAGC 3 cut(s) 31, 220, 227
BsgI GTGCAG 1 cut(s) 38
BshFI GGCC 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 103, 232
BslI CCNNNNNNNGG 1 cut(s) 182
BsmI GAATGC 1 cut(s) 69
BsnI GGCC 1 cut(s) 276
Bsp1286I GDGCHC 2 cut(s) 103, 232
Bsp143I GATC 1 cut(s) 294
BspANI GGCC 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 275
BspPI GGATC 1 cut(s) 289
BspQI GCTCTTC 1 cut(s) 108
BsrI ACTGG 1 cut(s) 316
BssMI GATC 1 cut(s) 294
Bst4CI ACNGT 2 cut(s) 89, 234
Bst6I CTCTTC 1 cut(s) 108
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 1 cut(s) 267
BstF5I GGATG 1 cut(s) 255
BstKTI GATC 1 cut(s) 297
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstSLI GKGCMC 1 cut(s) 232
BstV1I GCAGC 3 cut(s) 31, 220, 227
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BsuRI GGCC 1 cut(s) 276
BtsCI GGATG 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 230
Cac8I GCNNGC 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 80, 188
CviJI RGCY 6 cut(s) 22, 35, 41, 50, 101, 276
CviKI_1 RGCY 6 cut(s) 22, 35, 41, 50, 101, 276
CviQI GTAC 2 cut(s) 80, 188
DdeI CTNAG 1 cut(s) 267
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Ecl136II GAGCTC 1 cut(s) 101
Eco24I GRGCYC 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 101
EcoICRI GAGCTC 1 cut(s) 101
EcoT38I GRGCYC 1 cut(s) 103
FaiI YATR 1 cut(s) 147
FalI AAGNNNNNCTT 2 cut(s) 204, 236
Fnu4HI GCNGC 3 cut(s) 20, 209, 216
FokI GGATG 1 cut(s) 262
FriOI GRGCYC 1 cut(s) 103
Fsp4HI GCNGC 3 cut(s) 20, 209, 216
GluI GCNGC 3 cut(s) 20, 209, 216
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 1 cut(s) 276
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 2 cut(s) 188, 230
Hpy188III TCNNGA 4 cut(s) 109, 167, 193, 247
Hpy8I GTNNAC 2 cut(s) 188, 230
HpyCH4III ACNGT 2 cut(s) 89, 234
HpyCH4V TGCA 3 cut(s) 19, 208, 230
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 1 cut(s) 267
Kzo9I GATC 1 cut(s) 294
LguI GCTCTTC 1 cut(s) 108
LmnI GCTCC 2 cut(s) 38, 98
LpnPI CCDG 6 cut(s) 21, 47, 122, 180, 206, 297
Lsp1109I GCAGC 3 cut(s) 31, 220, 227
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 5 cut(s) 95, 232, 265, 301, 304
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 2 cut(s) 103, 232
MluCI AATT 2 cut(s) 119, 179
MnlI CCTC 3 cut(s) 59, 149, 264
MroXI GAANNNNTTC 1 cut(s) 200
MseI TTAA 1 cut(s) 84
MslI CAYNNNNRTG 1 cut(s) 248
MunI CAATTG 1 cut(s) 119
Mva1269I GAATGC 1 cut(s) 69
MwoI GCNNNNNNNGC 1 cut(s) 47
NdeII GATC 1 cut(s) 294
NlaIV GGNNCC 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 108
PctI GAATGC 1 cut(s) 69
PdmI GAANNNNTTC 1 cut(s) 200
PkrI GCNGC 3 cut(s) 21, 210, 217
Psp124BI GAGCTC 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 275
PspPI GGNCC 1 cut(s) 274
PsuI RGATCY 1 cut(s) 294
RsaI GTAC 2 cut(s) 81, 189
RsaNI GTAC 2 cut(s) 80, 188
RseI CAYNNNNRTG 1 cut(s) 248
SacI GAGCTC 1 cut(s) 103
SapI GCTCTTC 1 cut(s) 108
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 3 cut(s) 20, 209, 216
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 1 cut(s) 274
SduI GDGCHC 2 cut(s) 103, 232
SetI ASST 5 cut(s) 37, 43, 52, 61, 103
SmiMI CAYNNNNRTG 1 cut(s) 248
Sse9I AATT 2 cut(s) 119, 179
SspI AATATT 2 cut(s) 11, 94
SstI GAGCTC 1 cut(s) 103
TaaI ACNGT 2 cut(s) 89, 234
TasI AATT 2 cut(s) 119, 179
TatI WGTACW 2 cut(s) 79, 187
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 1 cut(s) 237
TseI GCWGC 3 cut(s) 19, 208, 215
TspDTI ATGAA 3 cut(s) 17, 232, 266
TspRI CASTG 1 cut(s) 237
VneI GTGCAC 1 cut(s) 228
XapI RAATTY 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.