FvH4_5g30450

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011782
Physical Location & Seq
Reverse (-)
21325051 .. 21327327
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30450.t1

Sequence Viewer

Length: 480 bp
ATGGGGTTCTTCTCCTTCTTCGGCCGCGTCCTCTTCTCCACCATCTTCATCCTCTCCGCTTGGCAAGCGTTCAATGAATTTGGTACTGATGGAGGACCTGCTGCAAAGGAGTTGGCTCCCAAATTTTCTGTTTTGAAAGATCACCTGTCTTCCAAACTCGGGTTTGCATTACCAGATGTCAATCCTATTCATTTGGCTGGAACTGTGATAGCTCTAAAAGGTCTGGGAGGCATTCTATTTGTACTTAACAGTAATATTGGAGCTCTTCTTCTGATATTTCAATTGGTTTTGACCACTCCCCTCATCTATGATTTCTACAACTTCAGTCCAGACACCCCCAAATTTGGTGTACTCTTGAATGATTTCTTGCAGCAAGCGGCACTTGTTGGTGCACTGTTTTTCTTCATAGGGATGAAGAACTCTATTACTAAGAGGGGGCAAGTCACCAAGAGGAAGATCCACAAAACGAAAACTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

160

Amino Acids

17.38

Weight (kDa)

9.87

Isoelectric Point (pI)

26.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 138 5.9e-50 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011782)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04340 AT5G43460 AT5G43460
fragaria_vesca FvH4_4g03211 FvH4_5g30450
malus_domestica MD13G1210700.v1.1 MD16G1213900.v1.1
prunus_persica Prupe.1G035100_v2.0.a1
pyrus_communis pycom13g18320 pycom16g17970
rosa_chinensis RchiOBHm_Chr4g0391891
rosa_laevigata RLG00000009851
rosa_multiflora Rmu_co8463711.1_g000001 Rmu_sc0000383.1_g000013 Rmu_sc0007693.1_g000011
rosa_rugosa Rorug03G0336700
rosa_samantha Rh4AG039100 Rh4BG033800
rosa_wichuraiana Rw4G003050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 106
AccII CGCG 1 cut(s) 27
AciI CCGC 3 cut(s) 25, 57, 377
AclWI GGATC 1 cut(s) 451
AcoI YGGCCR 1 cut(s) 22
AcsI RAATTY 3 cut(s) 77, 122, 341
AcuI CTGAAG 1 cut(s) 307
AfaI GTAC 3 cut(s) 85, 243, 351
AfiI CCNNNNNNNGG 2 cut(s) 159, 344
AgsI TTSAA 4 cut(s) 73, 136, 281, 358
AluBI AGCT 2 cut(s) 212, 263
AluI AGCT 2 cut(s) 212, 263
Alw21I GWGCWC 2 cut(s) 265, 394
Alw44I GTGCAC 1 cut(s) 390
AlwI GGATC 1 cut(s) 451
Ama87I CYCGRG 1 cut(s) 158
AoxI GGCC 1 cut(s) 22
ApaLI GTGCAC 1 cut(s) 390
ApeKI GCWGC 2 cut(s) 101, 370
ApoI RAATTY 3 cut(s) 77, 122, 341
Asp700I GAANNNNTTC 1 cut(s) 362
AspS9I GGNCC 1 cut(s) 95
AsuHPI GGTGA 2 cut(s) 134, 436
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 2 cut(s) 265, 394
BbvI GCAGC 2 cut(s) 88, 382
BccI CCATC 2 cut(s) 50, 83
BfuAI ACCTGC 1 cut(s) 106
BisI GCNGC 4 cut(s) 25, 102, 371, 378
BlsI GCNGC 4 cut(s) 26, 103, 372, 379
Bme18I GGWCC 1 cut(s) 95
BmeT110I CYCGRG 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 141
BsaBI GATNNNNATC 1 cut(s) 180
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 344
Bse1I ACTGG 1 cut(s) 478
Bse8I GATNNNNATC 1 cut(s) 180
BseGI GGATG 2 cut(s) 48, 417
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 159, 344
BseNI ACTGG 1 cut(s) 478
BseSI GKGCMC 1 cut(s) 394
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 88, 382
Bsh1236I CGCG 1 cut(s) 27
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 1 cut(s) 24
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 2 cut(s) 265, 394
BsiHKCI CYCGRG 1 cut(s) 158
BslI CCNNNNNNNGG 2 cut(s) 159, 344
BsmI GAATGC 1 cut(s) 231
BsnI GGCC 1 cut(s) 24
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 2 cut(s) 265, 394
Bsp143I GATC 2 cut(s) 139, 456
BspACI CCGC 3 cut(s) 25, 57, 377
BspANI GGCC 1 cut(s) 24
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 1 cut(s) 117
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 1 cut(s) 451
BspQI GCTCTTC 1 cut(s) 270
BsrI ACTGG 1 cut(s) 478
BssMI GATC 2 cut(s) 139, 456
Bst4CI ACNGT 3 cut(s) 205, 251, 396
Bst6I CTCTTC 2 cut(s) 38, 270
BstC8I GCNNGC 2 cut(s) 66, 375
BstDEI CTNAG 1 cut(s) 429
BstF5I GGATG 2 cut(s) 48, 417
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 2 cut(s) 142, 459
BstMBI GATC 2 cut(s) 139, 456
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstSLI GKGCMC 1 cut(s) 394
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 2 cut(s) 88, 382
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 1 cut(s) 24
BtsCI GGATG 2 cut(s) 48, 417
BtsIMutI CAGTG 1 cut(s) 392
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 2 cut(s) 66, 375
Cfr13I GGNCC 1 cut(s) 95
CseI GACGC 1 cut(s) 16
Csp6I GTAC 3 cut(s) 84, 242, 350
CviJI RGCY 5 cut(s) 24, 116, 197, 212, 263
CviKI_1 RGCY 5 cut(s) 24, 116, 197, 212, 263
CviQI GTAC 3 cut(s) 84, 242, 350
DdeI CTNAG 1 cut(s) 429
DpnI GATC 2 cut(s) 141, 458
DpnII GATC 2 cut(s) 139, 456
EaeI YGGCCR 1 cut(s) 22
EagI CGGCCG 1 cut(s) 22
Eam1104I CTCTTC 2 cut(s) 38, 270
EarI CTCTTC 2 cut(s) 38, 270
Ecl136II GAGCTC 1 cut(s) 263
EclXI CGGCCG 1 cut(s) 22
Eco24I GRGCYC 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 95
Eco52I CGGCCG 1 cut(s) 22
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 265
FaiI YATR 2 cut(s) 309, 407
FalI AAGNNNNNCTT 2 cut(s) 366, 398
Fnu4HI GCNGC 4 cut(s) 25, 102, 371, 378
FokI GGATG 2 cut(s) 35, 424
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 4 cut(s) 25, 102, 371, 378
GluI GCNGC 4 cut(s) 25, 102, 371, 378
HaeIII GGCC 1 cut(s) 24
HgaI GACGC 1 cut(s) 16
HphI GGTGA 2 cut(s) 134, 436
Hpy166II GTNNAC 2 cut(s) 350, 392
Hpy188I TCNGA 1 cut(s) 273
Hpy188III TCNNGA 2 cut(s) 329, 355
Hpy8I GTNNAC 2 cut(s) 350, 392
HpyAV CCTTC 1 cut(s) 25
HpyCH4III ACNGT 3 cut(s) 205, 251, 396
HpyCH4V TGCA 4 cut(s) 104, 167, 370, 392
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 1 cut(s) 429
Kzo9I GATC 2 cut(s) 139, 456
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 2 cut(s) 121, 260
LpnPI CCDG 7 cut(s) 111, 158, 183, 186, 209, 342, 459
Lsp1109I GCAGC 2 cut(s) 88, 382
MaeIII GTNAC 1 cut(s) 442
MalI GATC 2 cut(s) 141, 458
MboI GATC 2 cut(s) 139, 456
MboII GAAGA 9 cut(s) 10, 25, 37, 141, 257, 260, 394, 427, 466
MfeI CAATTG 1 cut(s) 281
MflI RGATCY 1 cut(s) 456
MhlI GDGCHC 2 cut(s) 265, 394
MluCI AATT 4 cut(s) 77, 122, 281, 341
MnlI CCTC 7 cut(s) 41, 62, 86, 221, 311, 426, 444
MroXI GAANNNNTTC 1 cut(s) 362
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 1 cut(s) 410
MunI CAATTG 1 cut(s) 281
Mva1269I GAATGC 1 cut(s) 231
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 65
NdeII GATC 2 cut(s) 139, 456
NlaIV GGNNCC 1 cut(s) 117
NmuCI GTSAC 1 cut(s) 442
PciSI GCTCTTC 1 cut(s) 270
PctI GAATGC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 362
PkrI GCNGC 4 cut(s) 26, 103, 372, 379
PpuMI RGGWCCY 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 265
Psp5II RGGWCCY 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 117
PspPI GGNCC 1 cut(s) 95
PspPPI RGGWCCY 1 cut(s) 95
PsuI RGATCY 1 cut(s) 456
RsaI GTAC 3 cut(s) 85, 243, 351
RsaNI GTAC 3 cut(s) 84, 242, 350
RseI CAYNNNNRTG 1 cut(s) 410
SacI GAGCTC 1 cut(s) 265
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 4 cut(s) 25, 102, 371, 378
Sau3AI GATC 2 cut(s) 139, 456
Sau96I GGNCC 1 cut(s) 95
SduI GDGCHC 2 cut(s) 265, 394
SetI ASST 5 cut(s) 100, 147, 214, 223, 265
SinI GGWCC 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 410
Sse9I AATT 4 cut(s) 77, 122, 281, 341
SsiI CCGC 3 cut(s) 25, 57, 377
SspI AATATT 1 cut(s) 256
SstI GAGCTC 1 cut(s) 265
TaaI ACNGT 3 cut(s) 205, 251, 396
TasI AATT 4 cut(s) 77, 122, 281, 341
TatI WGTACW 2 cut(s) 241, 349
TauI GCSGC 2 cut(s) 27, 380
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 1 cut(s) 399
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 2 cut(s) 101, 370
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 5 cut(s) 37, 90, 179, 394, 428
TspRI CASTG 1 cut(s) 399
VneI GTGCAC 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 95
XapI RAATTY 3 cut(s) 77, 122, 341
XmnI GAANNNNTTC 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.