AT5G44100
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006989
Physical Location & Seq
Reverse (-)
17749057 .. 17752849
3793 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44100.1

Sequence Viewer

Length: 1431 bp
ATGGATCTCGTGATCGGTGGGAAATTTAAGCTTGGGAAGAAAATTGGTAGTGGATCATTTGGAGAGCTTTATCTAGGTGTAAATGTCCAAACAGGAGAAGAAGTTGCTGTCAAGCTGGAAAATGTGAAGACCAAACATCCACAACTTCATTATGAGTCAAAGTTGTATATGCTGCTTCAAGGAGGATCTGGTATTCCGAACATCAAGTGGTTTGGAGTTGAAGGTGACTACAGCGTAATGGTTATTGACCTTCTAGGTCCTAGTCTTGAAGACTTGTTTAACTACTGCAACAGAAAGTTAACATTGAAAACTGTTCTCATGCTTGCCGACCAATTACTTAACAGGGTTGAGTTTATGCATACTCGAGGTTTCCTTCACCGTGATATAAAACCTGACAACTTTTTAATGGGCCTTGGACGCAAAGCAAATCAGGTGTATATCATTGATTTTGGACTAGGGAAGAAATACAGAGACCTTCAGACTCACAAGCACATTCCTTACAGAGAAAACAAAAATCTTACTGGGACAGCTCGGTATGCAAGTGTTAACACTCACCTTGGAGTCGAACAAAGTCGAAGGGATGATTTGGAATCACTTGGTTATGTTCTTATGTATTTTCTCAAAGGAAGCTTACCTTGGCAGGGATTAAAAGCTGGGACAAAGAAGCAGAAGTATGACAGAATCAGCGAGAAAAAAGTGTCAACTCCTATAGAGGTATTGTGTAAAAACCAACCGTCCGAGTTCGTTTCATACTTTCATTACTGCCGATCTTTACGGTTTGATGACAAACCTGATTACTCTTACCTCAAGAGGCTTTTCCGTGACTTGTTTATCCGAGAAGGTTATCAATTCGATTATGTATTTGACTGGACAGTCTTAAAGTATCCTCAAATCGGATCCAGCTCTGGTTCTAGTTCACGAACAAGGCATCATACAACAGCGAAACCCGGATTCAACGCAGATCCTATTGAAAGACAAGAGAGGATCCTAGGAAAGGAAACAACCCGATATAAAATCCCAGGTGCAGTGGAGGCATTCTCCAGAAGACACCCGACTACAACTTCTTCGCCTCGAGATCGTTCTAGATCAAGAAACTCAGACGACGGACCTTTCTCTAAGCAGACACATGGAGACTCCGAAAGAGCAAACAGTTCCTCAAGATATAGAGCATCTTCTTCTAGGAAAGCCGTTGCTGCTTCAAGCTCCCGGCCAAGTTCTGCAGGTGGACCAAGCGAAAGCCGAACTTCAAGTCGTCTGGTTTCCTCAAGCGGTGGTGGTGGCAGCGGCAGTGGAAATGGCCGTCCCTCAACTAGCCAGAGAGTGCAAGCTGGATATGAATCCAAGACTCTGTCTTTCTCTCGTGCAACAGCGGCAAGAAACACTCGGGAAGATCAATTAAGAAGCTTCGAGCTTCTCTCTCTTCGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0005911 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0008092 GO:0008150 GO:0008152 GO:0008360 GO:0009506 GO:0009987 GO:0012505 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0023052 GO:0030054 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0036211 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0046777 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051234 GO:0051510 GO:0051716 GO:0055028 GO:0055044 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071944 GO:0097435 GO:0098657 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04068 ko04310 ko04340 ko04341 ko04390 ko04391 ko04392 ko04710 ko04711 map04068 map04310 map04340 map04341 map04390 map04391 map04392 map04710 map04711
Pfam Domains
Protein Families
Pkinase

Protein Analysis

476

Amino Acids

53.59

Weight (kDa)

9.86

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 9 - 227 9e-26 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 10 - 269 4.6e-14 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 119 - 206 4.9e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1211
AasI GACNNNNNNGTC 1 cut(s) 872
Acc36I ACCTGC 1 cut(s) 1211
AciI CCGC 3 cut(s) 1269, 1284, 1370
AclWI GGATC 8 cut(s) 12, 61, 193, 891, 904, 956, 979, 992
AcoI YGGCCR 2 cut(s) 1208, 1297
AcsI RAATTY 1 cut(s) 23
AcuI CTGAAG 1 cut(s) 461
AfiI CCNNNNNNNGG 3 cut(s) 641, 893, 994
AgsI TTSAA 8 cut(s) 179, 221, 269, 307, 955, 971, 1200, 1248
AjnI CCWGG 1 cut(s) 1018
AjuI GAANNNNNNNTTGG 2 cut(s) 619, 651
Alw26I GTCTC 2 cut(s) 465, 1125
AlwI GGATC 8 cut(s) 12, 61, 193, 891, 904, 956, 979, 992
Ama87I CYCGRG 3 cut(s) 363, 1071, 1383
AoxI GGCC 3 cut(s) 409, 1208, 1297
ApeKI GCWGC 3 cut(s) 172, 1193, 1281
ApoI RAATTY 1 cut(s) 23
ArsI GACNNNNNNTTYG 2 cut(s) 1234, 1266
AspA2I CCTAGG 1 cut(s) 988
AspS9I GGNCC 4 cut(s) 257, 409, 1106, 1226
AsuC2I CCSGG 2 cut(s) 948, 1207
AsuHPI GGTGA 3 cut(s) 236, 368, 545
AvaI CYCGRG 3 cut(s) 363, 1071, 1383
AvaII GGWCC 3 cut(s) 257, 1106, 1226
AvrII CCTAGG 1 cut(s) 988
BamHI GGATCC 2 cut(s) 896, 984
BauI CACGAG 2 cut(s) 8, 1359
BbsI GAAGAC 3 cut(s) 134, 276, 1051
BbvI GCAGC 3 cut(s) 159, 1180, 1293
BceAI ACGGC 2 cut(s) 1172, 1284
BciT130I CCWGG 1 cut(s) 1020
BciVI GTATCC 1 cut(s) 894
BcnI CCSGG 2 cut(s) 948, 1207
BcoDI GTCTC 2 cut(s) 465, 1125
BfaI CTAG 9 cut(s) 74, 254, 261, 455, 912, 989, 1083, 1179, 1311
BfmI CTRYAG 3 cut(s) 229, 708, 1218
BfuAI ACCTGC 1 cut(s) 1211
BfuI GTATCC 1 cut(s) 894
BisI GCNGC 5 cut(s) 173, 1194, 1282, 1285, 1371
BlnI CCTAGG 1 cut(s) 988
BlsI GCNGC 5 cut(s) 174, 1195, 1283, 1286, 1372
Bme1390I CCNGG 3 cut(s) 948, 1020, 1207
Bme18I GGWCC 3 cut(s) 257, 1106, 1226
BmeT110I CYCGRG 3 cut(s) 363, 1071, 1383
BmgT120I GGNCC 4 cut(s) 257, 409, 1106, 1226
BmiI GGNNCC 2 cut(s) 898, 986
BmrFI CCNGG 3 cut(s) 948, 1020, 1207
BmrI ACTGGG 1 cut(s) 531
BmsI GCATC 2 cut(s) 937, 1178
BmuI ACTGGG 1 cut(s) 531
BpiI GAAGAC 3 cut(s) 134, 276, 1051
BplI GAGNNNNNCTC 3 cut(s) 1022, 1054, 1400
BpmI CTGGAG 1 cut(s) 1024
BpuEI CTTGAG 3 cut(s) 791, 1141, 1249
BpuMI CCSGG 2 cut(s) 948, 1207
BsaBI GATNNNNATC 1 cut(s) 1336
BsaI GGTCTC 1 cut(s) 465
BsaJI CCNNGG 5 cut(s) 412, 556, 635, 988, 1018
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 3 cut(s) 641, 893, 994
Bse1I ACTGG 2 cut(s) 526, 872
Bse8I GATNNNNATC 1 cut(s) 1336
BseBI CCWGG 1 cut(s) 1020
BseDI CCNNGG 5 cut(s) 412, 556, 635, 988, 1018
BseGI GGATG 2 cut(s) 136, 586
BseJI GATNNNNATC 1 cut(s) 1336
BseLI CCNNNNNNNGG 3 cut(s) 641, 893, 994
BseMII CTCAG 1 cut(s) 1110
BseNI ACTGG 2 cut(s) 526, 872
BseXI GCAGC 3 cut(s) 159, 1180, 1293
BseYI CCCAGC 1 cut(s) 653
BsgI GTGCAG 1 cut(s) 1044
BshFI GGCC 3 cut(s) 411, 1210, 1299
BsiHKCI CYCGRG 3 cut(s) 363, 1071, 1383
BsiSI CCGG 2 cut(s) 948, 1207
BslFI GGGAC 3 cut(s) 538, 670, 1287
BslI CCNNNNNNNGG 3 cut(s) 641, 893, 994
BsmAI GTCTC 2 cut(s) 465, 1125
BsmFI GGGAC 3 cut(s) 538, 670, 1287
BsmI GAATGC 1 cut(s) 1034
BsnI GGCC 3 cut(s) 411, 1210, 1299
Bso31I GGTCTC 1 cut(s) 465
BsoBI CYCGRG 3 cut(s) 363, 1071, 1383
BspACI CCGC 3 cut(s) 1269, 1284, 1370
BspANI GGCC 3 cut(s) 411, 1210, 1299
BspCNI CTCAG 1 cut(s) 1109
BspLI GGNNCC 2 cut(s) 898, 986
BspMAI CTGCAG 1 cut(s) 1222
BspMI ACCTGC 1 cut(s) 1211
BspPI GGATC 8 cut(s) 12, 61, 193, 891, 904, 956, 979, 992
BspTNI GGTCTC 1 cut(s) 465
BsrI ACTGG 2 cut(s) 526, 872
BssECI CCNNGG 5 cut(s) 412, 556, 635, 988, 1018
BssSI CACGAG 2 cut(s) 8, 1359
BssT1I CCWWGG 4 cut(s) 412, 556, 635, 988
Bst2BI CACGAG 2 cut(s) 8, 1359
Bst2UI CCWGG 1 cut(s) 1020
Bst4CI ACNGT 6 cut(s) 313, 380, 735, 777, 874, 1151
Bst6I CTCTTC 1 cut(s) 1425
BstC8I GCNNGC 2 cut(s) 324, 1326
BstDEI CTNAG 2 cut(s) 1096, 1116
BstENI CCTNNNNNAGG 1 cut(s) 992
BstF5I GGATG 2 cut(s) 136, 586
BstMAI GTCTC 2 cut(s) 465, 1125
BstMWI GCNNNNNNNGC 5 cut(s) 417, 536, 1031, 1193, 1370
BstNI CCWGG 1 cut(s) 1020
BstSCI CCNGG 3 cut(s) 946, 1018, 1205
BstSFI CTRYAG 3 cut(s) 229, 708, 1218
BstV1I GCAGC 3 cut(s) 159, 1180, 1293
BstV2I GAAGAC 3 cut(s) 134, 276, 1051
BstX2I RGATCY 5 cut(s) 4, 185, 896, 961, 984
BstYI RGATCY 5 cut(s) 4, 185, 896, 961, 984
BsuI GTATCC 1 cut(s) 894
BsuRI GGCC 3 cut(s) 411, 1210, 1299
BtsCI GGATG 2 cut(s) 136, 586
BtsI GCAGTG 2 cut(s) 1032, 1294
BtsIMutI CAGTG 2 cut(s) 1032, 1294
BveI ACCTGC 1 cut(s) 1211
Cac8I GCNNGC 2 cut(s) 324, 1326
Cfr13I GGNCC 4 cut(s) 257, 409, 1106, 1226
CseI GACGC 1 cut(s) 426
CviAII CATG 2 cut(s) 319, 1127
DdeI CTNAG 2 cut(s) 1096, 1116
DrdI GACNNNNNNGTC 1 cut(s) 872
DseDI GACNNNNNNGTC 1 cut(s) 872
EaeI YGGCCR 2 cut(s) 1208, 1297
Eam1104I CTCTTC 1 cut(s) 1425
EarI CTCTTC 1 cut(s) 1425
Eco130I CCWWGG 4 cut(s) 412, 556, 635, 988
Eco31I GGTCTC 1 cut(s) 465
Eco47I GGWCC 3 cut(s) 257, 1106, 1226
Eco57I CTGAAG 1 cut(s) 461
Eco88I CYCGRG 3 cut(s) 363, 1071, 1383
EcoNI CCTNNNNNAGG 1 cut(s) 992
EcoO109I RGGNCCY 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 1018
EcoT14I CCWWGG 4 cut(s) 412, 556, 635, 988
EcoT22I ATGCAT 1 cut(s) 360
ErhI CCWWGG 4 cut(s) 412, 556, 635, 988
FaeI CATG 2 cut(s) 322, 1130
FalI AAGNNNNNCTT 4 cut(s) 619, 651, 1228, 1260
FaqI GGGAC 3 cut(s) 538, 670, 1287
FatI CATG 2 cut(s) 318, 1126
Fnu4HI GCNGC 5 cut(s) 173, 1194, 1282, 1285, 1371
FokI GGATG 2 cut(s) 123, 593
Fsp4HI GCNGC 5 cut(s) 173, 1194, 1282, 1285, 1371
FspBI CTAG 9 cut(s) 74, 254, 261, 455, 912, 989, 1083, 1179, 1311
GluI GCNGC 5 cut(s) 173, 1194, 1282, 1285, 1371
GsaI CCCAGC 1 cut(s) 657
GsuI CTGGAG 1 cut(s) 1024
HaeIII GGCC 3 cut(s) 411, 1210, 1299
HapII CCGG 2 cut(s) 948, 1207
HgaI GACGC 1 cut(s) 426
Hin1II CATG 2 cut(s) 322, 1130
HincII GTYRAC 3 cut(s) 300, 547, 702
HindII GTYRAC 3 cut(s) 300, 547, 702
HindIII AAGCTT 3 cut(s) 29, 628, 1402
HinfI GANTC 9 cut(s) 155, 481, 561, 590, 681, 951, 1133, 1337, 1345
HpaI GTTAAC 2 cut(s) 300, 547
HpaII CCGG 2 cut(s) 948, 1207
HphI GGTGA 3 cut(s) 236, 368, 545
Hpy166II GTNNAC 5 cut(s) 300, 547, 702, 917, 1226
Hpy188I TCNGA 8 cut(s) 198, 480, 739, 836, 896, 1099, 1138, 1425
Hpy8I GTNNAC 5 cut(s) 300, 547, 702, 917, 1226
Hpy99I CGWCG 1 cut(s) 1106
HpyAV CCTTC 6 cut(s) 215, 260, 383, 485, 570, 833
HpyCH4III ACNGT 6 cut(s) 313, 380, 735, 777, 874, 1151
HpyCH4V TGCA 7 cut(s) 288, 358, 539, 1025, 1220, 1324, 1364
HpyF10VI GCNNNNNNNGC 5 cut(s) 417, 536, 1031, 1193, 1370
HpyF3I CTNAG 2 cut(s) 1096, 1116
Hsp92II CATG 2 cut(s) 322, 1130
KspAI GTTAAC 2 cut(s) 300, 547
LmnI GCTCC 1 cut(s) 1208
Lsp1109I GCAGC 3 cut(s) 159, 1180, 1293
LweI GCATC 2 cut(s) 937, 1178
MaeI CTAG 9 cut(s) 74, 254, 261, 455, 912, 989, 1083, 1179, 1311
MaeIII GTNAC 2 cut(s) 224, 821
MflI RGATCY 5 cut(s) 4, 185, 896, 961, 984
MluCI AATT 5 cut(s) 23, 42, 332, 848, 1394
MlyI GAGTC 5 cut(s) 164, 475, 570, 1127, 1339
Mph1103I ATGCAT 1 cut(s) 360
MseI TTAA 9 cut(s) 27, 279, 299, 339, 404, 546, 647, 878, 1397
MspA1I CMGCKG 2 cut(s) 1284, 1370
MspI CCGG 2 cut(s) 948, 1207
MspR9I CCNGG 3 cut(s) 948, 1020, 1207
Mva1269I GAATGC 1 cut(s) 1034
MvaI CCWGG 1 cut(s) 1020
MwoI GCNNNNNNNGC 5 cut(s) 417, 536, 1031, 1193, 1370
NciI CCSGG 2 cut(s) 948, 1207
NlaIII CATG 2 cut(s) 322, 1130
NlaIV GGNNCC 2 cut(s) 898, 986
NmuCI GTSAC 2 cut(s) 224, 821
NsiI ATGCAT 1 cut(s) 360
PaeR7I CTCGAG 2 cut(s) 363, 1071
PaqCI CACCTGC 1 cut(s) 1211
PctI GAATGC 1 cut(s) 1034
PfeI GAWTC 4 cut(s) 590, 681, 951, 1337
PflFI GACNNNGTC 1 cut(s) 1348
PkrI GCNGC 5 cut(s) 174, 1195, 1283, 1286, 1372
PleI GAGTC 5 cut(s) 163, 475, 569, 1127, 1339
PpsI GAGTC 5 cut(s) 163, 475, 569, 1127, 1339
PpuMI RGGWCCY 1 cut(s) 257
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 1018
PspFI CCCAGC 1 cut(s) 653
PspGI CCWGG 1 cut(s) 1018
PspN4I GGNNCC 2 cut(s) 898, 986
PspPI GGNCC 4 cut(s) 257, 409, 1106, 1226
PspPPI RGGWCCY 1 cut(s) 257
PspXI VCTCGAGB 1 cut(s) 363
PstI CTGCAG 1 cut(s) 1222
PsuI RGATCY 5 cut(s) 4, 185, 896, 961, 984
PsyI GACNNNGTC 1 cut(s) 1348
SaqAI TTAA 9 cut(s) 27, 279, 299, 339, 404, 546, 647, 878, 1397
SatI GCNGC 5 cut(s) 173, 1194, 1282, 1285, 1371
Sau96I GGNCC 4 cut(s) 257, 409, 1106, 1226
SchI GAGTC 5 cut(s) 164, 475, 570, 1127, 1339
ScrFI CCNGG 3 cut(s) 948, 1020, 1207
SfaNI GCATC 2 cut(s) 937, 1178
SfcI CTRYAG 3 cut(s) 229, 708, 1218
Sfr274I CTCGAG 2 cut(s) 363, 1071
SinI GGWCC 3 cut(s) 257, 1106, 1226
SlaI CTCGAG 2 cut(s) 363, 1071
SmlI CTYRAG 5 cut(s) 363, 806, 1071, 1156, 1264
SmoI CTYRAG 5 cut(s) 363, 806, 1071, 1156, 1264
Sse9I AATT 5 cut(s) 23, 42, 332, 848, 1394
SsiI CCGC 3 cut(s) 1269, 1284, 1370
SspMI CTAG 9 cut(s) 74, 254, 261, 455, 912, 989, 1083, 1179, 1311
StyD4I CCNGG 3 cut(s) 946, 1018, 1205
StyI CCWWGG 4 cut(s) 412, 556, 635, 988
TaaI ACNGT 6 cut(s) 313, 380, 735, 777, 874, 1151
TaqI TCGA 6 cut(s) 364, 564, 574, 852, 1072, 1407
TasI AATT 5 cut(s) 23, 42, 332, 848, 1394
TauI GCSGC 2 cut(s) 1287, 1373
TfiI GAWTC 4 cut(s) 590, 681, 951, 1337
Tru1I TTAA 9 cut(s) 27, 279, 299, 339, 404, 546, 647, 878, 1397
Tru9I TTAA 9 cut(s) 27, 279, 299, 339, 404, 546, 647, 878, 1397
TscAI CASTG 2 cut(s) 1032, 1294
TseFI GTSAC 2 cut(s) 224, 821
TseI GCWGC 3 cut(s) 172, 1193, 1281
Tsp45I GTSAC 2 cut(s) 224, 821
TspDTI ATGAA 4 cut(s) 137, 738, 746, 1350
TspGWI ACGGA 2 cut(s) 809, 1119
TspRI CASTG 2 cut(s) 1032, 1294
Tth111I GACNNNGTC 1 cut(s) 1348
VpaK11BI GGWCC 3 cut(s) 257, 1106, 1226
XagI CCTNNNNNAGG 1 cut(s) 992
XapI RAATTY 1 cut(s) 23
XbaI TCTAGA 1 cut(s) 1082
XhoI CTCGAG 2 cut(s) 363, 1071
XmaJI CCTAGG 1 cut(s) 988
XspI CTAG 9 cut(s) 74, 254, 261, 455, 912, 989, 1083, 1179, 1311
Zsp2I ATGCAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.