AT5G44680

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012860
Physical Location & Seq
Reverse (-)
18023985 .. 18026077
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44680.1

Sequence Viewer

Length: 1062 bp
ATGTGTTCCTCAAAATTAAAAAATCTGACCCAAGAAAACATTTCTCAGATCAACGGCCGTCCGGTTCTTCAACCGAAGTCGAACCAAGTTCCGACATTAGACCGACGTAACTCACTAAAAAAATCGCCTCCTAAGCCTTTGAATCCAATTGCATCAAAGATACCCTCACCAAGACCAATTTCTCTAATCTCTCCTCCTTTGTCTCCCAACACAAAATCCTTAAGAAAACCGGCCGGTTCTTGCAAGGAACTTCTTAGGTCTAGCTCTACAAAATCGAAACCAGTGATATCACCAGAGAACAGTGATGGTGGCTACAAAGAGGTTATGCCTATGGTGATTGTTCAAAAGCAGCCTGGAAGTATAGCAGCTGCCAGAAGAGAAGAGGTTGCAATGAAACAAGAAGAGAGAAAGAAGAAAATTTCTCATTACGGTAGAATCAAATCCGTAAAATCGAATGAAAAGAACTTAAATGTTGAGCATGAGAAGAAGAAGAGATGCAGTTTCATCACTACAAGTTCAGATCCAATATATGTCGCTTACCATGACAAAGAATGGGGAGTTCCAGTTCATGATGACAATCTACTATTTGAGCTTCTGGTGTTAACCGGAGCTCAGGTCGGATCAGATTGGACTTCGGTTTTAAAGAGAAGAAACACTTTTAGAGAGGCTTTCTCTGGTTTCGAAGCAGAGTTGGTCGCAGACTTCAACGAGAAGAAGATACAATCAATAGTCAACGACTATGGCATTAACCTTAGCCAAGTCCTTGCAGTTGTTGACAACGCTAAACAAATTCTCAAGGTGAAAAGAGATTTGGGGTCATTCAACAAATACATTTGGGGGTTTATGAAACACAAACCGGTCACCACAAAATACACATCATGCCAAAAGATACCGGTTAAGACATCGAAATCGGAGACTATAAGCAAAGACATGGTTCGTCGTGGGTTTAGATTCGTTGGTCCGACGGTTATACATTCGTTAATGCAAGCCGCCGGTTTAACCAACGACCACTTGATCACATGTCCGAGGCATCTCGAGTGTACGGCCATGGCGGCTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

353

Amino Acids

39.51

Weight (kDa)

9.87

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 172 - 344 6.1e-66 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012860)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44680
fragaria_vesca FvH4_3g13300
malus_domestica MD05G1243000.v1.1 MD10G1225100.v1.1
prunus_persica Prupe.4G120400_v2.0.a1
pyrus_communis pycom05g21960 pycom10g18890
rosa_chinensis RchiOBHm_Chr5g0021771
rosa_laevigata RLG00000032626
rosa_multiflora Rmu_ssc0000233.1_g000002
rosa_roxburghii Rroxscaffold_1G00056940
rosa_rugosa Rorug05G0069300 Rorug05G0069400
rosa_samantha Rh5AG157900 Rh5BG157400 Rh5CG171600 Rh5DG157700
rosa_wichuraiana Rw5G014060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 990, 1052
AclWI GGATC 2 cut(s) 515, 628
AcoI YGGCCR 3 cut(s) 55, 231, 1044
AcsI RAATTY 2 cut(s) 417, 789
AfaI GTAC 1 cut(s) 1042
AflII CTTAAG 1 cut(s) 220
AflIII ACRYGT 1 cut(s) 1019
AgeI ACCGGT 2 cut(s) 856, 892
AgsI TTSAA 5 cut(s) 71, 142, 344, 706, 823
AjnI CCWGG 1 cut(s) 352
AjuI GAANNNNNNNTTGG 4 cut(s) 163, 195, 794, 826
AloI GAACNNNNNNTCC 2 cut(s) 549, 581
AluBI AGCT 4 cut(s) 264, 368, 592, 611
AluI AGCT 4 cut(s) 264, 368, 592, 611
Alw21I GWGCWC 1 cut(s) 613
Alw26I GTCTC 2 cut(s) 207, 908
AlwI GGATC 2 cut(s) 515, 628
Ama87I CYCGRG 1 cut(s) 1034
AoxI GGCC 3 cut(s) 55, 231, 1044
ApeKI GCWGC 3 cut(s) 349, 365, 368
ApoI RAATTY 2 cut(s) 417, 789
AsiGI ACCGGT 2 cut(s) 856, 892
AspS9I GGNCC 1 cut(s) 959
AsuHPI GGTGA 5 cut(s) 159, 282, 346, 811, 853
AsuII TTCGAA 1 cut(s) 681
AvaI CYCGRG 1 cut(s) 1034
AvaII GGWCC 1 cut(s) 959
BanII GRGCYC 1 cut(s) 613
Bbv12I GWGCWC 1 cut(s) 613
BbvI GCAGC 3 cut(s) 355, 361, 377
BccI CCATC 1 cut(s) 299
BceAI ACGGC 3 cut(s) 42, 70, 1059
BciT130I CCWGG 1 cut(s) 354
BclI TGATCA 1 cut(s) 1014
BcoDI GTCTC 2 cut(s) 207, 908
BfaI CTAG 1 cut(s) 261
BfrI CTTAAG 1 cut(s) 220
BglI GCCNNNNNGGC 1 cut(s) 1052
BisI GCNGC 5 cut(s) 350, 366, 369, 990, 1053
BlsI GCNGC 5 cut(s) 351, 367, 370, 991, 1054
Bme1390I CCNGG 1 cut(s) 354
Bme18I GGWCC 1 cut(s) 959
BmeT110I CYCGRG 1 cut(s) 1034
BmgT120I GGNCC 1 cut(s) 959
BmrFI CCNGG 1 cut(s) 354
BmsI GCATC 3 cut(s) 161, 485, 1039
BplI GAGNNNNNCTC 2 cut(s) 656, 688
Bpu10I CCTNAGC 3 cut(s) 132, 612, 752
Bpu14I TTCGAA 1 cut(s) 681
BpuEI CTTGAG 1 cut(s) 779
BsaBI GATNNNNATC 1 cut(s) 576
BsaJI CCNNGG 2 cut(s) 1025, 1047
BsaWI WCCGGW 4 cut(s) 61, 605, 856, 892
BsaXI ACNNNNNCTCC 4 cut(s) 549, 579, 600, 630
Bse118I RCCGGY 5 cut(s) 229, 233, 856, 892, 992
Bse1I ACTGG 2 cut(s) 281, 563
Bse3DI GCAATG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 2 cut(s) 1025, 1047
BseJI GATNNNNATC 1 cut(s) 576
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 2 cut(s) 59, 626
BseNI ACTGG 2 cut(s) 281, 563
BseRI GAGGAG 1 cut(s) 183
BseX3I CGGCCG 2 cut(s) 55, 231
BseXI GCAGC 3 cut(s) 355, 361, 377
Bsh1285I CGRYCG 2 cut(s) 58, 234
BshFI GGCC 3 cut(s) 57, 233, 1046
BshTI ACCGGT 2 cut(s) 856, 892
BsiEI CGRYCG 2 cut(s) 58, 234
BsiHKAI GWGCWC 1 cut(s) 613
BsiHKCI CYCGRG 1 cut(s) 1034
BsiSI CCGG 7 cut(s) 62, 230, 234, 606, 857, 893, 993
BsmAI GTCTC 2 cut(s) 207, 908
BsnI GGCC 3 cut(s) 57, 233, 1046
BsoBI CYCGRG 1 cut(s) 1034
Bsp119I TTCGAA 1 cut(s) 681
Bsp1286I GDGCHC 1 cut(s) 613
Bsp143I GATC 4 cut(s) 48, 520, 620, 1014
Bsp19I CCATGG 1 cut(s) 1047
BspACI CCGC 2 cut(s) 990, 1052
BspANI GGCC 3 cut(s) 57, 233, 1046
BspCNI CTCAG 2 cut(s) 58, 625
BspHI TCATGA 1 cut(s) 568
BspPI GGATC 2 cut(s) 515, 628
BspT104I TTCGAA 1 cut(s) 681
BspTI CTTAAG 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 396
BsrFI RCCGGY 5 cut(s) 229, 233, 856, 892, 992
BsrI ACTGG 2 cut(s) 281, 563
BssAI RCCGGY 5 cut(s) 229, 233, 856, 892, 992
BssECI CCNNGG 2 cut(s) 1025, 1047
BssMI GATC 4 cut(s) 48, 520, 620, 1014
BssT1I CCWWGG 1 cut(s) 1047
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 3 cut(s) 302, 431, 967
Bst6I CTCTTC 4 cut(s) 370, 375, 396, 485
BstAFI CTTAAG 1 cut(s) 220
BstBI TTCGAA 1 cut(s) 681
BstC8I GCNNGC 1 cut(s) 987
BstDEI CTNAG 5 cut(s) 45, 132, 254, 612, 752
BstDSI CCRYGG 1 cut(s) 1047
BstEII GGTNACC 1 cut(s) 859
BstKTI GATC 4 cut(s) 51, 523, 623, 1017
BstMAI GTCTC 2 cut(s) 207, 908
BstMBI GATC 4 cut(s) 48, 520, 620, 1014
BstMCI CGRYCG 2 cut(s) 58, 234
BstMWI GCNNNNNNNGC 2 cut(s) 133, 1052
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 1 cut(s) 1023
BstPI GGTNACC 1 cut(s) 859
BstSCI CCNGG 1 cut(s) 352
BstV1I GCAGC 3 cut(s) 355, 361, 377
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BstZI CGGCCG 2 cut(s) 55, 231
BsuRI GGCC 3 cut(s) 57, 233, 1046
BtgI CCRYGG 1 cut(s) 1047
BtsIMutI CAGTG 2 cut(s) 288, 307
Cac8I GCNNGC 1 cut(s) 987
CciI TCATGA 1 cut(s) 568
Cfr10I RCCGGY 5 cut(s) 229, 233, 856, 892, 992
Cfr13I GGNCC 1 cut(s) 959
Csp6I GTAC 1 cut(s) 1041
CspAI ACCGGT 2 cut(s) 856, 892
CviAII CATG 7 cut(s) 479, 542, 569, 879, 931, 1020, 1048
CviQI GTAC 1 cut(s) 1041
DdeI CTNAG 5 cut(s) 45, 132, 254, 612, 752
DpnI GATC 4 cut(s) 50, 522, 622, 1016
DpnII GATC 4 cut(s) 48, 520, 620, 1014
DraI TTTAAA 1 cut(s) 642
EaeI YGGCCR 3 cut(s) 55, 231, 1044
EagI CGGCCG 2 cut(s) 55, 231
Eam1104I CTCTTC 4 cut(s) 370, 375, 396, 485
EarI CTCTTC 4 cut(s) 370, 375, 396, 485
Ecl136II GAGCTC 1 cut(s) 611
EclXI CGGCCG 2 cut(s) 55, 231
Eco130I CCWWGG 1 cut(s) 1047
Eco24I GRGCYC 1 cut(s) 613
Eco32I GATATC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 959
Eco52I CGGCCG 2 cut(s) 55, 231
Eco53kI GAGCTC 1 cut(s) 611
Eco88I CYCGRG 1 cut(s) 1034
Eco91I GGTNACC 1 cut(s) 859
EcoICRI GAGCTC 1 cut(s) 611
EcoO65I GGTNACC 1 cut(s) 859
EcoRII CCWGG 1 cut(s) 352
EcoRV GATATC 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 1047
EcoT38I GRGCYC 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 1047
FaeI CATG 7 cut(s) 482, 545, 572, 882, 934, 1023, 1051
FalI AAGNNNNNCTT 2 cut(s) 640, 672
FatI CATG 7 cut(s) 478, 541, 568, 878, 930, 1019, 1047
FbaI TGATCA 1 cut(s) 1014
Fnu4HI GCNGC 5 cut(s) 350, 366, 369, 990, 1053
FriOI GRGCYC 1 cut(s) 613
Fsp4HI GCNGC 5 cut(s) 350, 366, 369, 990, 1053
FspBI CTAG 1 cut(s) 261
GluI GCNGC 5 cut(s) 350, 366, 369, 990, 1053
HaeIII GGCC 3 cut(s) 57, 233, 1046
HapII CCGG 7 cut(s) 62, 230, 234, 606, 857, 893, 993
Hin1II CATG 7 cut(s) 482, 545, 572, 882, 934, 1023, 1051
HincII GTYRAC 3 cut(s) 603, 733, 775
HindII GTYRAC 3 cut(s) 603, 733, 775
HinfI GANTC 3 cut(s) 142, 435, 951
HpaI GTTAAC 1 cut(s) 603
HpaII CCGG 7 cut(s) 62, 230, 234, 606, 857, 893, 993
HphI GGTGA 5 cut(s) 159, 282, 346, 811, 853
Hpy166II GTNNAC 4 cut(s) 603, 733, 775, 1041
Hpy188I TCNGA 9 cut(s) 27, 48, 93, 520, 620, 625, 913, 963, 1026
Hpy188III TCNNGA 2 cut(s) 569, 1034
Hpy8I GTNNAC 4 cut(s) 603, 733, 775, 1041
Hpy99I CGWCG 3 cut(s) 108, 942, 967
HpyCH4III ACNGT 3 cut(s) 302, 431, 967
HpyCH4IV ACGT 1 cut(s) 106
HpyCH4V TGCA 6 cut(s) 152, 243, 389, 498, 767, 985
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 1052
HpyF3I CTNAG 5 cut(s) 45, 132, 254, 612, 752
HpySE526I ACGT 1 cut(s) 106
Hsp92II CATG 7 cut(s) 482, 545, 572, 882, 934, 1023, 1051
Ksp22I TGATCA 1 cut(s) 1014
KspAI GTTAAC 1 cut(s) 603
Kzo9I GATC 4 cut(s) 48, 520, 620, 1014
LmnI GCTCC 1 cut(s) 608
Lsp1109I GCAGC 3 cut(s) 355, 361, 377
LweI GCATC 3 cut(s) 161, 485, 1039
MaeI CTAG 1 cut(s) 261
MaeII ACGT 1 cut(s) 106
MaeIII GTNAC 2 cut(s) 107, 859
MalI GATC 4 cut(s) 50, 522, 622, 1016
MboI GATC 4 cut(s) 48, 520, 620, 1014
MfeI CAATTG 1 cut(s) 147
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 1 cut(s) 613
MluCI AATT 5 cut(s) 14, 147, 177, 417, 789
MmeI TCCRAC 3 cut(s) 116, 598, 986
MnlI CCTC 8 cut(s) 19, 138, 175, 204, 313, 376, 658, 1020
MseI TTAA 9 cut(s) 17, 221, 467, 602, 641, 747, 897, 980, 998
MspA1I CMGCKG 1 cut(s) 368
MspCI CTTAAG 1 cut(s) 220
MspI CCGG 7 cut(s) 62, 230, 234, 606, 857, 893, 993
MspR9I CCNGG 1 cut(s) 354
MunI CAATTG 1 cut(s) 147
MvaI CCWGG 1 cut(s) 354
MwoI GCNNNNNNNGC 2 cut(s) 133, 1052
NcoI CCATGG 1 cut(s) 1047
NdeII GATC 4 cut(s) 48, 520, 620, 1014
NlaIII CATG 7 cut(s) 482, 545, 572, 882, 934, 1023, 1051
NmuCI GTSAC 1 cut(s) 859
NspI RCATGY 1 cut(s) 1023
NspV TTCGAA 1 cut(s) 681
PaeR7I CTCGAG 1 cut(s) 1034
PagI TCATGA 1 cut(s) 568
PciI ACATGT 1 cut(s) 1019
PfeI GAWTC 3 cut(s) 142, 435, 951
PinAI ACCGGT 2 cut(s) 856, 892
PkrI GCNGC 5 cut(s) 351, 367, 370, 991, 1054
PscI ACATGT 1 cut(s) 1019
Psp124BI GAGCTC 1 cut(s) 613
Psp6I CCWGG 1 cut(s) 352
PspEI GGTNACC 1 cut(s) 859
PspGI CCWGG 1 cut(s) 352
PspPI GGNCC 1 cut(s) 959
PsuI RGATCY 1 cut(s) 520
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 1 cut(s) 1042
RsaNI GTAC 1 cut(s) 1041
SacI GAGCTC 1 cut(s) 613
SaqAI TTAA 9 cut(s) 17, 221, 467, 602, 641, 747, 897, 980, 998
SatI GCNGC 5 cut(s) 350, 366, 369, 990, 1053
Sau3AI GATC 4 cut(s) 48, 520, 620, 1014
Sau96I GGNCC 1 cut(s) 959
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 1 cut(s) 613
SfaNI GCATC 3 cut(s) 161, 485, 1039
Sfr274I CTCGAG 1 cut(s) 1034
SfuI TTCGAA 1 cut(s) 681
SinI GGWCC 1 cut(s) 959
SlaI CTCGAG 1 cut(s) 1034
SmlI CTYRAG 3 cut(s) 220, 794, 1034
SmoI CTYRAG 3 cut(s) 220, 794, 1034
Sse9I AATT 5 cut(s) 14, 147, 177, 417, 789
SsiI CCGC 2 cut(s) 990, 1052
SspMI CTAG 1 cut(s) 261
SstI GAGCTC 1 cut(s) 613
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 1 cut(s) 1047
TaaI ACNGT 3 cut(s) 302, 431, 967
TaiI ACGT 1 cut(s) 109
TaqI TCGA 6 cut(s) 80, 275, 452, 681, 905, 1035
TaqII GACCGA 1 cut(s) 117
TasI AATT 5 cut(s) 14, 147, 177, 417, 789
TauI GCSGC 2 cut(s) 992, 1055
TfiI GAWTC 3 cut(s) 142, 435, 951
Tru1I TTAA 9 cut(s) 17, 221, 467, 602, 641, 747, 897, 980, 998
Tru9I TTAA 9 cut(s) 17, 221, 467, 602, 641, 747, 897, 980, 998
TscAI CASTG 2 cut(s) 288, 307
TseFI GTSAC 1 cut(s) 859
TseI GCWGC 3 cut(s) 349, 365, 368
Tsp45I GTSAC 1 cut(s) 859
TspDTI ATGAA 5 cut(s) 407, 471, 493, 557, 860
TspGWI ACGGA 1 cut(s) 433
TspRI CASTG 2 cut(s) 288, 307
Vha464I CTTAAG 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 959
XapI RAATTY 2 cut(s) 417, 789
XceI RCATGY 1 cut(s) 1023
XhoI CTCGAG 1 cut(s) 1034
XspI CTAG 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.