MD05G1243000.v1.1

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012860
Physical Location & Seq
Reverse (-)
37576760 .. 37579361
2602 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1243000.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGTGTTCCGCGAAACCAAGATTGCAACGAAGTGTTAGTGTCTCTGCAACATCAGGGAAACTAATCAACCGGCGTCCGGTTCTCCAGCCAACCGGCAATCAATTTCCGAGCTTGGAACAAAAAAAGTCTCCTAAAAAAAGCTCCCAAGAAACTAATGGTCCAACTCCTCTTCCTTCACCACTTCCAACTAATAAAACTAAGGCCTCACTTTCTCCTCCTATCTCTCCCAAGTTGCCTTCACCTCGACAGCATGCGTTTAAGCAAGGTAAAGATCCCAATGAGCTGAATTCTAGTGCTGAGAAGGTTGTAGTGACGCCTCGTTGCGTAACCAAATCGACAAGTTCGGTGAAAAAGTCGAAGAAATCTATTGGTGTTGCACCTTCTGCTGAAAGTGTGTTGAAGAATATATCGCCTTTGATAGTAGAGGCACCAGGAAGCATAGCAGCAACAAGGAGGGAGCAAGTAGCAACAATGCAAGAACAGCGAAAGATGCGAATTGCGCATTACGGAAGAACAAAGTCTGCCAAGTACGAAGGGAAAGTTGTTCCTCTTGATTCTTCAGCTACAAATTATTTCGGTCAAGAGCAAAGGAGATGCAGTTTCATCACCCCTAAGTCAGACCCAATATATGTTGCTTACCATGATGAAGAATGGGGAGTTCCTGTCCATGATGACAATGTGTTGCTTGAATTGCTACTATTAACTGGTGCTCAAGTGGGATCGGATTGGACTTCAGTCCTGAGGAAAAGGGAAGCATTGAGGGAGGCTTTTTCAGGGTTTGATGCAGATGTTGTGGCTAGATTTAGTGAAAAAAAGATAACATCATTGAGTGCTGATTCTGGCGTAGATATTAGCCTTGTTCGAGGAGTTGTTGACAATGCTAAGAGAATTCTTAAGGTAAAGAGGGAAGTGGGATCATTTGACGAGTACTTGTGGGGATTTGTGAATCACAAGCCAATATGTACCCAATACAAGTCATGCCACAAGATTCCTGTGAAGAACTCCAAGTCAGAGGCCATCAGCAAAGACATGGTTAGAAGGGGGTTTAGGCATGTTGGTCCCACTGTAATTCACTTCTTCATGCAAGCAGCTGGCCTCACCAATGACCACTTGACCACCTGCCCAAGACACCCCCACTGCATCCCTTCACCATCATCACCCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

390

Amino Acids

42.82

Weight (kDa)

9.88

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 205 - 377 1.3e-62 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012860)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44680
fragaria_vesca FvH4_3g13300
malus_domestica MD05G1243000.v1.1 MD10G1225100.v1.1
prunus_persica Prupe.4G120400_v2.0.a1
pyrus_communis pycom05g21960 pycom10g18890
rosa_chinensis RchiOBHm_Chr5g0021771
rosa_laevigata RLG00000032626
rosa_multiflora Rmu_ssc0000233.1_g000002
rosa_roxburghii Rroxscaffold_1G00056940
rosa_rugosa Rorug05G0069300 Rorug05G0069400
rosa_samantha Rh5AG157900 Rh5BG157400 Rh5CG171600 Rh5DG157700
rosa_wichuraiana Rw5G014060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1127
Acc16I TGCGCA 1 cut(s) 501
Acc36I ACCTGC 1 cut(s) 1127
AccB1I GGYRCC 1 cut(s) 427
AccII CGCG 1 cut(s) 11
AciI CCGC 1 cut(s) 9
AclWI GGATC 3 cut(s) 266, 727, 922
AcsI RAATTY 2 cut(s) 286, 888
AcuI CTGAAG 2 cut(s) 543, 717
AcyI GRCGYC 2 cut(s) 73, 314
AfaI GTAC 3 cut(s) 530, 929, 964
AfiI CCNNNNNNNGG 1 cut(s) 76
AflII CTTAAG 1 cut(s) 893
AgsI TTSAA 2 cut(s) 400, 689
AjnI CCWGG 1 cut(s) 430
AluBI AGCT 5 cut(s) 111, 141, 283, 563, 1091
AluI AGCT 5 cut(s) 111, 141, 283, 563, 1091
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 2 cut(s) 46, 132
AlwI GGATC 3 cut(s) 266, 727, 922
AoxI GGCC 3 cut(s) 201, 1014, 1093
ApeKI GCWGC 2 cut(s) 443, 1088
ApoI RAATTY 2 cut(s) 286, 888
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 2 cut(s) 158, 1058
AsuHPI GGTGA 7 cut(s) 168, 231, 358, 598, 1090, 1140, 1149
AvaII GGWCC 2 cut(s) 158, 1058
AxyI CCTNAGG 1 cut(s) 740
BanI GGYRCC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 2 cut(s) 455, 1100
BccI CCATC 2 cut(s) 1025, 1159
BciT130I CCWGG 1 cut(s) 432
BcoDI GTCTC 2 cut(s) 46, 132
BfaI CTAG 2 cut(s) 291, 798
BfrI CTTAAG 1 cut(s) 893
BfuAI ACCTGC 1 cut(s) 1127
BisI GCNGC 2 cut(s) 444, 1089
BlsI GCNGC 2 cut(s) 445, 1090
BmcAI AGTACT 1 cut(s) 929
Bme1390I CCNGG 1 cut(s) 432
Bme18I GGWCC 2 cut(s) 158, 1058
BmgT120I GGNCC 2 cut(s) 158, 1058
BmiI GGNNCC 2 cut(s) 429, 1060
BmrFI CCNGG 1 cut(s) 432
BmsI GCATC 4 cut(s) 480, 584, 772, 1149
BoxI GACNNNNGTC 1 cut(s) 734
BpmI CTGGAG 1 cut(s) 68
BpuEI CTTGAG 1 cut(s) 696
BsaHI GRCGYC 2 cut(s) 73, 314
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 4 cut(s) 583, 613, 648, 678
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse118I RCCGGY 2 cut(s) 69, 92
Bse1I ACTGG 1 cut(s) 709
Bse21I CCTNAGG 1 cut(s) 740
BseBI CCWGG 1 cut(s) 432
BseGI GGATG 1 cut(s) 1140
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMII CTCAG 2 cut(s) 288, 731
BseNI ACTGG 1 cut(s) 709
BseRI GAGGAG 3 cut(s) 156, 204, 879
BseXI GCAGC 2 cut(s) 455, 1100
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 3 cut(s) 203, 1016, 1095
BshNI GGYRCC 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 712
BsiSI CCGG 3 cut(s) 70, 77, 93
BslFI GGGAC 1 cut(s) 1044
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 2 cut(s) 46, 132
BsmFI GGGAC 1 cut(s) 1044
BsnI GGCC 3 cut(s) 203, 1016, 1095
Bsp1286I GDGCHC 1 cut(s) 712
Bsp143I GATC 3 cut(s) 271, 719, 914
BspACI CCGC 1 cut(s) 9
BspANI GGCC 3 cut(s) 203, 1016, 1095
BspCNI CTCAG 2 cut(s) 289, 732
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 2 cut(s) 429, 1060
BspMI ACCTGC 1 cut(s) 1127
BspPI GGATC 3 cut(s) 266, 727, 922
BspT107I GGYRCC 1 cut(s) 427
BspTI CTTAAG 1 cut(s) 893
BsrFI RCCGGY 2 cut(s) 69, 92
BsrI ACTGG 1 cut(s) 709
BssAI RCCGGY 2 cut(s) 69, 92
BssMI GATC 3 cut(s) 271, 719, 914
BssNI GRCGYC 2 cut(s) 73, 314
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 1 cut(s) 1066
Bst6I CTCTTC 1 cut(s) 174
BstACI GRCGYC 2 cut(s) 73, 314
BstAFI CTTAAG 1 cut(s) 893
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 3 cut(s) 252, 1086, 1093
BstDEI CTNAG 5 cut(s) 198, 297, 612, 740, 882
BstF5I GGATG 1 cut(s) 1140
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 3 cut(s) 274, 722, 917
BstMAI GTCTC 2 cut(s) 46, 132
BstMBI GATC 3 cut(s) 271, 719, 914
BstMWI GCNNNNNNNGC 5 cut(s) 383, 481, 490, 499, 691
BstNI CCWGG 1 cut(s) 432
BstNSI RCATGY 2 cut(s) 254, 1055
BstPAI GACNNNNGTC 1 cut(s) 734
BstSCI CCNGG 1 cut(s) 430
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 455, 1100
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
Bsu36I CCTNAGG 1 cut(s) 740
BsuRI GGCC 3 cut(s) 203, 1016, 1095
BtsCI GGATG 1 cut(s) 1140
BtsI GCAGTG 1 cut(s) 1135
BtsIMutI CAGTG 2 cut(s) 1062, 1135
BveI ACCTGC 1 cut(s) 1127
Cac8I GCNNGC 3 cut(s) 252, 1086, 1093
CfoI GCGC 1 cut(s) 502
Cfr10I RCCGGY 2 cut(s) 69, 92
Cfr13I GGNCC 2 cut(s) 158, 1058
CseI GACGC 2 cut(s) 62, 322
Csp6I GTAC 3 cut(s) 529, 928, 963
CviAII CATG 7 cut(s) 251, 641, 668, 978, 1030, 1052, 1081
CviQI GTAC 3 cut(s) 529, 928, 963
DdeI CTNAG 5 cut(s) 198, 297, 612, 740, 882
DpnI GATC 3 cut(s) 273, 721, 916
DpnII GATC 3 cut(s) 271, 719, 914
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco147I AGGCCT 1 cut(s) 203
Eco47I GGWCC 2 cut(s) 158, 1058
Eco57I CTGAAG 2 cut(s) 543, 717
Eco81I CCTNAGG 1 cut(s) 740
EcoRI GAATTC 2 cut(s) 286, 888
EcoRII CCWGG 1 cut(s) 430
FaeI CATG 7 cut(s) 254, 644, 671, 981, 1033, 1055, 1084
FaqI GGGAC 1 cut(s) 1044
FatI CATG 7 cut(s) 250, 640, 667, 977, 1029, 1051, 1080
Fnu4HI GCNGC 2 cut(s) 444, 1089
FokI GGATG 1 cut(s) 1127
Fsp4HI GCNGC 2 cut(s) 444, 1089
FspBI CTAG 2 cut(s) 291, 798
FspI TGCGCA 1 cut(s) 501
GlaI GCGC 1 cut(s) 501
GluI GCNGC 2 cut(s) 444, 1089
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 3 cut(s) 203, 1016, 1095
HapII CCGG 3 cut(s) 70, 77, 93
HgaI GACGC 2 cut(s) 62, 322
HhaI GCGC 1 cut(s) 502
Hin1I GRCGYC 2 cut(s) 73, 314
Hin1II CATG 7 cut(s) 254, 644, 671, 981, 1033, 1055, 1084
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 1 cut(s) 874
HindII GTYRAC 1 cut(s) 874
HinfI GANTC 4 cut(s) 554, 836, 946, 988
HpaII CCGG 3 cut(s) 70, 77, 93
HphI GGTGA 7 cut(s) 168, 231, 358, 598, 1090, 1140, 1149
Hpy166II GTNNAC 1 cut(s) 874
Hpy188I TCNGA 4 cut(s) 108, 619, 724, 1012
Hpy188III TCNNGA 3 cut(s) 551, 581, 739
Hpy8I GTNNAC 1 cut(s) 874
HpyAV CCTTC 7 cut(s) 183, 246, 295, 390, 527, 1032, 1155
HpyCH4III ACNGT 1 cut(s) 1066
HpyCH4V TGCA 8 cut(s) 25, 47, 377, 475, 597, 785, 1084, 1140
HpyF10VI GCNNNNNNNGC 5 cut(s) 383, 481, 490, 499, 691
HpyF3I CTNAG 5 cut(s) 198, 297, 612, 740, 882
Hsp92I GRCGYC 2 cut(s) 73, 314
Hsp92II CATG 7 cut(s) 254, 644, 671, 981, 1033, 1055, 1084
HspAI GCGC 1 cut(s) 500
Kzo9I GATC 3 cut(s) 271, 719, 914
LmnI GCTCC 2 cut(s) 146, 457
Lsp1109I GCAGC 2 cut(s) 455, 1100
LweI GCATC 4 cut(s) 480, 584, 772, 1149
MaeI CTAG 2 cut(s) 291, 798
MaeIII GTNAC 2 cut(s) 310, 325
MalI GATC 3 cut(s) 273, 721, 916
MboI GATC 3 cut(s) 271, 719, 914
MboII GAAGA 8 cut(s) 161, 370, 412, 522, 549, 659, 1009, 1069
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 1 cut(s) 712
MluCI AATT 7 cut(s) 101, 286, 495, 568, 689, 888, 1068
MmeI TCCRAC 2 cut(s) 185, 209
MseI TTAA 3 cut(s) 258, 701, 894
MspA1I CMGCKG 1 cut(s) 1091
MspCI CTTAAG 1 cut(s) 893
MspI CCGG 3 cut(s) 70, 77, 93
MspR9I CCNGG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 432
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 5 cut(s) 383, 481, 490, 499, 691
NdeII GATC 3 cut(s) 271, 719, 914
NlaIII CATG 7 cut(s) 254, 644, 671, 981, 1033, 1055, 1084
NlaIV GGNNCC 2 cut(s) 429, 1060
NmuCI GTSAC 1 cut(s) 310
NsbI TGCGCA 1 cut(s) 501
NspI RCATGY 2 cut(s) 254, 1055
PaeI GCATGC 1 cut(s) 254
PaqCI CACCTGC 1 cut(s) 1127
PceI AGGCCT 1 cut(s) 203
PfeI GAWTC 4 cut(s) 554, 836, 946, 988
PkrI GCNGC 2 cut(s) 445, 1090
PshAI GACNNNNGTC 1 cut(s) 734
Psp6I CCWGG 1 cut(s) 430
PspGI CCWGG 1 cut(s) 430
PspN4I GGNNCC 2 cut(s) 429, 1060
PspPI GGNCC 2 cut(s) 158, 1058
PsuI RGATCY 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 1091
RsaI GTAC 3 cut(s) 530, 929, 964
RsaNI GTAC 3 cut(s) 529, 928, 963
SaqAI TTAA 3 cut(s) 258, 701, 894
SatI GCNGC 2 cut(s) 444, 1089
Sau3AI GATC 3 cut(s) 271, 719, 914
Sau96I GGNCC 2 cut(s) 158, 1058
ScaI AGTACT 1 cut(s) 929
ScrFI CCNGG 1 cut(s) 432
SduI GDGCHC 1 cut(s) 712
SfaNI GCATC 4 cut(s) 480, 584, 772, 1149
SinI GGWCC 2 cut(s) 158, 1058
SmlI CTYRAG 2 cut(s) 711, 893
SmoI CTYRAG 2 cut(s) 711, 893
SphI GCATGC 1 cut(s) 254
Sse9I AATT 7 cut(s) 101, 286, 495, 568, 689, 888, 1068
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 1 cut(s) 9
SspMI CTAG 2 cut(s) 291, 798
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 430
TaaI ACNGT 1 cut(s) 1066
TaqI TCGA 4 cut(s) 244, 335, 356, 862
TaqII GACCGA 1 cut(s) 566
TasI AATT 7 cut(s) 101, 286, 495, 568, 689, 888, 1068
TatI WGTACW 1 cut(s) 927
TfiI GAWTC 4 cut(s) 554, 836, 946, 988
Tru1I TTAA 3 cut(s) 258, 701, 894
Tru9I TTAA 3 cut(s) 258, 701, 894
TscAI CASTG 2 cut(s) 1069, 1142
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 2 cut(s) 443, 1088
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 3 cut(s) 592, 660, 1069
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 2 cut(s) 1069, 1142
Vha464I CTTAAG 1 cut(s) 893
VpaK11BI GGWCC 2 cut(s) 158, 1058
XapI RAATTY 2 cut(s) 286, 888
XceI RCATGY 2 cut(s) 254, 1055
XcmI CCANNNNNNNNNTGG 1 cut(s) 152
XspI CTAG 2 cut(s) 291, 798
ZrmI AGTACT 1 cut(s) 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.