Rh5BG157400

glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012860
Physical Location & Seq
Forward (+)
16055114 .. 16057801
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG157400.1

Sequence Viewer

Length: 762 bp
ATGTGTTCCTCTAAACCAAAATTGCAGCAAAGTCCTAGTGTTACTCCAACAACACCTAAAACCAATCGCCGTTTGGTTCTTCAGCCAACTGGTAACCAATTTCCAAGCTTGGAACAAAAGAAGTTTCTCAAAAAGACCTCCCATGAATCCCTTATTCCACCTCCTTTGCTATCACCACTGCCATCACCGAAAACTAAGCCCGCAATTTCTCCTCCCATCTCTCCCAAGTTACCTTCGCCGCGACCACCAGCACTTAAGAGAGGAAAAGACCATAATGAGCTCAATTCTAGTCTTGAGAAGTTAATTCTCACACCTAAGTCAACAACCAAGTTGACTGGTTCAGTGAAAAAGTCAAAGAGATCAAGTGTTGCAGGTGCAGCTTCTGCTGAAAATGTAATGAAAAATATATCGTCTTTGATAGTAGAGGCACCAGGAAGCATAGCAGCTGCAAGGAGAGAGCAAGTTGCAACAATGCAAGAACAACGAAAGTTGAGAATTGCGCATTATGGAAGAACAAAGTCTGCAAAGTTTGAAGGGAAAGTAGTGCCTCTTGATACTTCATCCACCAATGTTGAACAAAGGAGATGCAGTTTTATCACGCCAAATTCAGACCCTCTGTATGTTGCTTACCATGATCAAGAATGGGGAGTTCCTGTTCATGATGACAATTTGTTGCTTGAATTGCTACTGCTGACCAGTGCTCAAGTAGGATCAGATTGGAGCTCGGTCCTGAGGAAAAGGCAGGCTTTGAGGTGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

253

Amino Acids

27.85

Weight (kDa)

10.22

Isoelectric Point (pI)

60.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 202 - 251 4e-16 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012860)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44680
fragaria_vesca FvH4_3g13300
malus_domestica MD05G1243000.v1.1 MD10G1225100.v1.1
prunus_persica Prupe.4G120400_v2.0.a1
pyrus_communis pycom05g21960 pycom10g18890
rosa_chinensis RchiOBHm_Chr5g0021771
rosa_laevigata RLG00000032626
rosa_multiflora Rmu_ssc0000233.1_g000002
rosa_roxburghii Rroxscaffold_1G00056940
rosa_rugosa Rorug05G0069300 Rorug05G0069400
rosa_samantha Rh5AG157900 Rh5BG157400 Rh5CG171600 Rh5DG157700
rosa_wichuraiana Rw5G014060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 362
Acc16I TGCGCA 1 cut(s) 501
Acc36I ACCTGC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 427
AccII CGCG 1 cut(s) 241
AciI CCGC 2 cut(s) 201, 239
AclWI GGATC 1 cut(s) 718
AcsI RAATTY 1 cut(s) 604
AcuI CTGAAG 1 cut(s) 65
AflII CTTAAG 1 cut(s) 254
AgsI TTSAA 3 cut(s) 533, 575, 680
AjnI CCWGG 1 cut(s) 430
AloI GAACNNNNNNTCC 2 cut(s) 639, 671
AluBI AGCT 5 cut(s) 108, 280, 380, 446, 723
AluI AGCT 5 cut(s) 108, 280, 380, 446, 723
Alw21I GWGCWC 3 cut(s) 282, 703, 725
AlwI GGATC 1 cut(s) 718
AlwNI CAGNNNCTG 1 cut(s) 383
ApeKI GCWGC 4 cut(s) 25, 377, 443, 446
ApoI RAATTY 1 cut(s) 604
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 1 cut(s) 727
AsuHPI GGTGA 2 cut(s) 165, 177
AvaII GGWCC 1 cut(s) 727
AxyI CCTNAGG 1 cut(s) 731
BanI GGYRCC 1 cut(s) 427
BanII GRGCYC 2 cut(s) 282, 725
Bbv12I GWGCWC 3 cut(s) 282, 703, 725
BbvI GCAGC 4 cut(s) 37, 389, 433, 455
BccI CCATC 2 cut(s) 190, 224
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 1 cut(s) 432
BclI TGATCA 1 cut(s) 634
BfaI CTAG 2 cut(s) 36, 288
BfrI CTTAAG 1 cut(s) 254
BfuAI ACCTGC 1 cut(s) 362
BisI GCNGC 5 cut(s) 26, 239, 378, 444, 447
BlsI GCNGC 5 cut(s) 27, 240, 379, 445, 448
Bme1390I CCNGG 1 cut(s) 432
Bme18I GGWCC 1 cut(s) 727
BmgT120I GGNCC 1 cut(s) 727
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 1 cut(s) 432
BmsI GCATC 1 cut(s) 575
BpuEI CTTGAG 2 cut(s) 314, 687
BsaXI ACNNNNNCTCC 4 cut(s) 574, 604, 639, 669
Bse1I ACTGG 3 cut(s) 94, 340, 696
Bse21I CCTNAGG 1 cut(s) 731
BseBI CCWGG 1 cut(s) 432
BseGI GGATG 1 cut(s) 560
BseMII CTCAG 1 cut(s) 722
BseNI ACTGG 3 cut(s) 94, 340, 696
BseRI GAGGAG 1 cut(s) 201
BseXI GCAGC 4 cut(s) 37, 389, 433, 455
BsgI GTGCAG 1 cut(s) 396
Bsh1236I CGCG 1 cut(s) 241
BshNI GGYRCC 1 cut(s) 427
BsiHKAI GWGCWC 3 cut(s) 282, 703, 725
Bsp1286I GDGCHC 3 cut(s) 282, 703, 725
Bsp143I GATC 3 cut(s) 359, 634, 710
BspACI CCGC 2 cut(s) 201, 239
BspCNI CTCAG 1 cut(s) 723
BspFNI CGCG 1 cut(s) 241
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 1 cut(s) 429
BspMI ACCTGC 1 cut(s) 362
BspPI GGATC 1 cut(s) 718
BspT107I GGYRCC 1 cut(s) 427
BspTI CTTAAG 1 cut(s) 254
BsrI ACTGG 3 cut(s) 94, 340, 696
BssMI GATC 3 cut(s) 359, 634, 710
Bst2UI CCWGG 1 cut(s) 432
BstAFI CTTAAG 1 cut(s) 254
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 2 cut(s) 201, 744
BstDEI CTNAG 3 cut(s) 195, 315, 731
BstEII GGTNACC 1 cut(s) 92
BstF5I GGATG 1 cut(s) 560
BstFNI CGCG 1 cut(s) 241
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 3 cut(s) 362, 637, 713
BstMBI GATC 3 cut(s) 359, 634, 710
BstMWI GCNNNNNNNGC 3 cut(s) 377, 383, 682
BstNI CCWGG 1 cut(s) 432
BstPI GGTNACC 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 430
BstUI CGCG 1 cut(s) 241
BstV1I GCAGC 4 cut(s) 37, 389, 433, 455
Bsu36I CCTNAGG 1 cut(s) 731
BtsCI GGATG 1 cut(s) 560
BtsI GCAGTG 1 cut(s) 176
BtsIMutI CAGTG 3 cut(s) 176, 348, 703
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 2 cut(s) 201, 744
CaiI CAGNNNCTG 1 cut(s) 383
CciI TCATGA 1 cut(s) 658
CfoI GCGC 1 cut(s) 502
Cfr13I GGNCC 1 cut(s) 727
CspCI CAANNNNNGTGG 4 cut(s) 147, 182, 553, 588
CviAII CATG 3 cut(s) 143, 632, 659
CviJI RGCY 8 cut(s) 85, 108, 199, 280, 380, 446, 723, 746
CviKI_1 RGCY 8 cut(s) 85, 108, 199, 280, 380, 446, 723, 746
DdeI CTNAG 3 cut(s) 195, 315, 731
DpnI GATC 3 cut(s) 361, 636, 712
DpnII GATC 3 cut(s) 359, 634, 710
Ecl136II GAGCTC 2 cut(s) 280, 723
Eco24I GRGCYC 2 cut(s) 282, 725
Eco47I GGWCC 1 cut(s) 727
Eco53kI GAGCTC 2 cut(s) 280, 723
Eco57I CTGAAG 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 731
Eco91I GGTNACC 1 cut(s) 92
EcoICRI GAGCTC 2 cut(s) 280, 723
EcoO65I GGTNACC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 430
EcoT38I GRGCYC 2 cut(s) 282, 725
FaeI CATG 3 cut(s) 146, 635, 662
FaiI YATR 8 cut(s) 144, 273, 407, 440, 507, 621, 633, 660
FalI AAGNNNNNCTT 2 cut(s) 730, 762
FatI CATG 3 cut(s) 142, 631, 658
FauI CCCGC 1 cut(s) 208
FbaI TGATCA 1 cut(s) 634
Fnu4HI GCNGC 5 cut(s) 26, 239, 378, 444, 447
FokI GGATG 1 cut(s) 547
FriOI GRGCYC 2 cut(s) 282, 725
Fsp4HI GCNGC 5 cut(s) 26, 239, 378, 444, 447
FspBI CTAG 2 cut(s) 36, 288
FspI TGCGCA 1 cut(s) 501
GlaI GCGC 1 cut(s) 501
GluI GCNGC 5 cut(s) 26, 239, 378, 444, 447
HhaI GCGC 1 cut(s) 502
Hin1II CATG 3 cut(s) 146, 635, 662
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 2 cut(s) 321, 333
HindII GTYRAC 2 cut(s) 321, 333
HindIII AAGCTT 1 cut(s) 106
HinfI GANTC 1 cut(s) 146
HphI GGTGA 2 cut(s) 165, 177
Hpy166II GTNNAC 2 cut(s) 321, 333
Hpy188I TCNGA 2 cut(s) 610, 715
Hpy188III TCNNGA 5 cut(s) 293, 551, 638, 659, 730
Hpy8I GTNNAC 2 cut(s) 321, 333
HpyAV CCTTC 2 cut(s) 243, 527
HpyCH4V TGCA 8 cut(s) 25, 371, 377, 449, 467, 475, 524, 588
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 383, 682
HpyF3I CTNAG 3 cut(s) 195, 315, 731
Hsp92II CATG 3 cut(s) 146, 635, 662
HspAI GCGC 1 cut(s) 500
Ksp22I TGATCA 1 cut(s) 634
Kzo9I GATC 3 cut(s) 359, 634, 710
LmnI GCTCC 1 cut(s) 720
Lsp1109I GCAGC 4 cut(s) 37, 389, 433, 455
LweI GCATC 1 cut(s) 575
MaeI CTAG 2 cut(s) 36, 288
MaeIII GTNAC 3 cut(s) 40, 92, 228
MalI GATC 3 cut(s) 361, 636, 712
MboI GATC 3 cut(s) 359, 634, 710
MboII GAAGA 2 cut(s) 71, 522
MhlI GDGCHC 3 cut(s) 282, 703, 725
MluCI AATT 9 cut(s) 20, 98, 204, 283, 303, 495, 604, 667, 680
MmeI TCCRAC 1 cut(s) 71
MseI TTAA 2 cut(s) 255, 302
MspA1I CMGCKG 1 cut(s) 446
MspCI CTTAAG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 432
MvnI CGCG 1 cut(s) 241
MwoI GCNNNNNNNGC 3 cut(s) 377, 383, 682
NdeII GATC 3 cut(s) 359, 634, 710
NlaIII CATG 3 cut(s) 146, 635, 662
NlaIV GGNNCC 1 cut(s) 429
NsbI TGCGCA 1 cut(s) 501
PagI TCATGA 1 cut(s) 658
PaqCI CACCTGC 1 cut(s) 362
PfeI GAWTC 1 cut(s) 146
PkrI GCNGC 5 cut(s) 27, 240, 379, 445, 448
Psp124BI GAGCTC 2 cut(s) 282, 725
Psp6I CCWGG 1 cut(s) 430
PspEI GGTNACC 1 cut(s) 92
PspGI CCWGG 1 cut(s) 430
PspN4I GGNNCC 1 cut(s) 429
PspPI GGNCC 1 cut(s) 727
PstNI CAGNNNCTG 1 cut(s) 383
PvuII CAGCTG 1 cut(s) 446
SacI GAGCTC 2 cut(s) 282, 725
SaqAI TTAA 2 cut(s) 255, 302
SatI GCNGC 5 cut(s) 26, 239, 378, 444, 447
Sau3AI GATC 3 cut(s) 359, 634, 710
Sau96I GGNCC 1 cut(s) 727
ScrFI CCNGG 1 cut(s) 432
SduI GDGCHC 3 cut(s) 282, 703, 725
SfaNI GCATC 1 cut(s) 575
SinI GGWCC 1 cut(s) 727
SmlI CTYRAG 3 cut(s) 254, 293, 702
SmoI CTYRAG 3 cut(s) 254, 293, 702
Sse9I AATT 9 cut(s) 20, 98, 204, 283, 303, 495, 604, 667, 680
SsiI CCGC 2 cut(s) 201, 239
SspMI CTAG 2 cut(s) 36, 288
SstI GAGCTC 2 cut(s) 282, 725
StyD4I CCNGG 1 cut(s) 430
TaqII GACCGA 1 cut(s) 715
TasI AATT 9 cut(s) 20, 98, 204, 283, 303, 495, 604, 667, 680
TauI GCSGC 1 cut(s) 241
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 2 cut(s) 255, 302
Tru9I TTAA 2 cut(s) 255, 302
TscAI CASTG 3 cut(s) 183, 348, 703
TseI GCWGC 4 cut(s) 25, 377, 443, 446
TspDTI ATGAA 4 cut(s) 159, 413, 549, 647
TspRI CASTG 3 cut(s) 183, 348, 703
Vha464I CTTAAG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 727
XapI RAATTY 1 cut(s) 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XspI CTAG 2 cut(s) 36, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.