Prupe.4G120400_v2.0.a1

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0012860
Physical Location & Seq
Forward (+)
6520184 .. 6522700
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G120400.1

Sequence Viewer

Length: 1194 bp
ATGTGTTCCTCTAAACCAAAGTTGCAGAGAACCACTAGTGTTCCTCCATCAACACCTAAGATGAATCGCCGTCCGGTTCTCCAGCCAACTGGTAATCAATTTCCAAGCCTGGAACAAAGGAAGTCTCTTAAAAAGAGCTCCCAAGAACCTCTTGCTCCAACTCCTCTGCCTTCACCTCTACCAAGTGCTAAAACTAAGGCCTCTCTTTCTCCTCCCATATCTCCCAAGTTGCCTTCACCTAGACCACCTGCATTCAAGAGAGGAAAAGATCCAAATGAGCTGAATTCTAGTGCTGAGAAGGTTGTGACACCTCGGTGCACAACCAAGTTTACAAGTTCTGTGAAAAAGTCAAAGAAGTCTAGTGGCTCTGTAGCTGCTGCACCTTCTGCTGAAAGTATATTGAAAAATATATCCTCTTTGATAGTGGAGGCACCAGGAAGCATAGCAGCTGCAAGGAGGGAGCAAGTTGCAACAATGCAAGAACAACGAAAGATGCGAATTGCCCATTATGGAAGAACCAAGTCTGCCAAAAATGAAGGGAAAGTGGTTCCTCTTGATGCTTCACCTACAACTGATTTTGGTAGAGACCAAAGGCGGTGTACTTTTATCACACCTAACTCAGACCCTATATATGTTGCTTACCATGATGAAGAATGGGGAGTTCCTGTCCATGATGACAATCTGTTGCTTGAATTGCTGGTGTTAACTGGTGCTCAAGTTGGATCAGATTGGACTTCAGTCCTGAGGAAAAGGCAAGCTTTGAGGGAGTCCTTTTCAGGATTTGATGCAGATGGTGTGGCTAAATTTAGTGAAAGAAAGATAACATCAGTGAGTTCTGATTCTGGTATAGATATAAGCCTTGTTCGAGGAGCTGTTGACAATGCTAAACGAATTCTTCAGATAAAGAGGGAAGTTGGATCATTTGACAAGTACTTGTGGGGATTTGTGAACCACAAGCCTATATCTACCCAATACAAGTCATGCCACAAGATCCCTGTGAAGAACTCCAAGTCAGAGAGCATAAGCAAAGACATGGTTAGAAGGGGTTTTAGGCTTGTTGGTCCCACAGTCATTCACTCATTCATGCAAGCAGCTGGCCTCACAAATGACCACTTGATCACCTGCCCACGGCACCTCCAATGTGCAGCTTCTTTGGCTTCAAGCCCTCCTGTTGCAGCCCCTGCTGCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

398

Amino Acids

43.19

Weight (kDa)

10.03

Isoelectric Point (pI)

56.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012860)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44680
fragaria_vesca FvH4_3g13300
malus_domestica MD05G1243000.v1.1 MD10G1225100.v1.1
prunus_persica Prupe.4G120400_v2.0.a1
pyrus_communis pycom05g21960 pycom10g18890
rosa_chinensis RchiOBHm_Chr5g0021771
rosa_laevigata RLG00000032626
rosa_multiflora Rmu_ssc0000233.1_g000002
rosa_roxburghii Rroxscaffold_1G00056940
rosa_rugosa Rorug05G0069300 Rorug05G0069400
rosa_samantha Rh5AG157900 Rh5BG157400 Rh5CG171600 Rh5DG157700
rosa_wichuraiana Rw5G014060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1192
AarI CACCTGC 2 cut(s) 256, 1130
Acc36I ACCTGC 2 cut(s) 256, 1130
AccB1I GGYRCC 2 cut(s) 430, 1131
AciI CCGC 1 cut(s) 595
AclWI GGATC 4 cut(s) 263, 730, 925, 985
AcsI RAATTY 3 cut(s) 283, 803, 891
AcuI CTGAAG 2 cut(s) 720, 881
AfaI GTAC 2 cut(s) 601, 932
AfiI CCNNNNNNNGG 1 cut(s) 1128
AgsI TTSAA 4 cut(s) 256, 403, 692, 1161
AhlI ACTAGT 1 cut(s) 35
AjnI CCWGG 2 cut(s) 108, 433
AleI CACNNNNGTG 1 cut(s) 313
AluBI AGCT 8 cut(s) 138, 280, 374, 449, 758, 872, 1094, 1148
AluI AGCT 8 cut(s) 138, 280, 374, 449, 758, 872, 1094, 1148
Alw21I GWGCWC 3 cut(s) 140, 320, 715
Alw26I GTCTC 2 cut(s) 129, 579
Alw44I GTGCAC 1 cut(s) 316
AlwI GGATC 4 cut(s) 263, 730, 925, 985
AlwNI CAGNNNCTG 1 cut(s) 1181
AoxI GGCC 2 cut(s) 198, 1096
ApaLI GTGCAC 1 cut(s) 316
ApeKI GCWGC 8 cut(s) 374, 377, 446, 449, 1091, 1145, 1175, 1184
ApoI RAATTY 3 cut(s) 283, 803, 891
AspS9I GGNCC 1 cut(s) 1061
AsuHPI GGTGA 4 cut(s) 165, 228, 555, 1111
AvaII GGWCC 1 cut(s) 1061
AxyI CCTNAGG 1 cut(s) 743
BaeGI GKGCMC 1 cut(s) 320
BanI GGYRCC 2 cut(s) 430, 1131
BanII GRGCYC 1 cut(s) 140
Bbv12I GWGCWC 3 cut(s) 140, 320, 715
BbvI GCAGC 8 cut(s) 361, 364, 436, 458, 1103, 1157, 1171, 1187
BccI CCATC 2 cut(s) 55, 785
BceAI ACGGC 2 cut(s) 54, 1145
BciT130I CCWGG 2 cut(s) 110, 435
BclI TGATCA 1 cut(s) 1116
BcoDI GTCTC 2 cut(s) 129, 579
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 4 cut(s) 36, 240, 288, 360
BfmI CTRYAG 1 cut(s) 369
BfuAI ACCTGC 2 cut(s) 256, 1130
BisI GCNGC 8 cut(s) 375, 378, 447, 450, 1092, 1146, 1176, 1185
BlsI GCNGC 8 cut(s) 376, 379, 448, 451, 1093, 1147, 1177, 1186
BmcAI AGTACT 1 cut(s) 932
Bme1390I CCNGG 2 cut(s) 110, 435
Bme18I GGWCC 1 cut(s) 1061
BmgT120I GGNCC 1 cut(s) 1061
BmiI GGNNCC 4 cut(s) 432, 549, 1063, 1133
BmrFI CCNGG 2 cut(s) 110, 435
BmsI GCATC 3 cut(s) 483, 547, 775
BoxI GACNNNNGTC 1 cut(s) 737
BpmI CTGGAG 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 699
BsaBI GATNNNNATC 1 cut(s) 678
BsaI GGTCTC 1 cut(s) 579
BsaJI CCNNGG 2 cut(s) 311, 1127
BsaWI WCCGGW 1 cut(s) 73
BsaXI ACNNNNNCTCC 4 cut(s) 651, 681, 1119, 1149
Bsc4I CCNNNNNNNGG 1 cut(s) 1128
Bse1I ACTGG 2 cut(s) 94, 712
Bse21I CCTNAGG 1 cut(s) 743
Bse8I GATNNNNATC 1 cut(s) 678
BseBI CCWGG 2 cut(s) 110, 435
BseDI CCNNGG 2 cut(s) 311, 1127
BseJI GATNNNNATC 1 cut(s) 678
BseLI CCNNNNNNNGG 1 cut(s) 1128
BseMII CTCAG 3 cut(s) 285, 633, 734
BseNI ACTGG 2 cut(s) 94, 712
BseRI GAGGAG 3 cut(s) 153, 201, 882
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 8 cut(s) 361, 364, 436, 458, 1103, 1157, 1171, 1187
BsgI GTGCAG 2 cut(s) 363, 1164
BshFI GGCC 2 cut(s) 200, 1098
BshNI GGYRCC 2 cut(s) 430, 1131
BsiHKAI GWGCWC 3 cut(s) 140, 320, 715
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 1047
BslI CCNNNNNNNGG 1 cut(s) 1128
BsmAI GTCTC 2 cut(s) 129, 579
BsmFI GGGAC 1 cut(s) 1047
BsmI GAATGC 1 cut(s) 251
BsnI GGCC 2 cut(s) 200, 1098
Bso31I GGTCTC 1 cut(s) 579
Bsp1286I GDGCHC 3 cut(s) 140, 320, 715
Bsp143I GATC 5 cut(s) 268, 722, 917, 990, 1116
BspACI CCGC 1 cut(s) 595
BspANI GGCC 2 cut(s) 200, 1098
BspCNI CTCAG 3 cut(s) 286, 632, 735
BspLI GGNNCC 4 cut(s) 432, 549, 1063, 1133
BspMI ACCTGC 2 cut(s) 256, 1130
BspPI GGATC 4 cut(s) 263, 730, 925, 985
BspT107I GGYRCC 2 cut(s) 430, 1131
BspTNI GGTCTC 1 cut(s) 579
BsrI ACTGG 2 cut(s) 94, 712
BssECI CCNNGG 2 cut(s) 311, 1127
BssMI GATC 5 cut(s) 268, 722, 917, 990, 1116
Bst2UI CCWGG 2 cut(s) 110, 435
Bst4CI ACNGT 1 cut(s) 1069
BstAPI GCANNNNNTGC 2 cut(s) 386, 1181
BstC8I GCNNGC 3 cut(s) 756, 1089, 1096
BstDEI CTNAG 5 cut(s) 57, 195, 294, 619, 743
BstDSI CCRYGG 1 cut(s) 1127
BstKTI GATC 5 cut(s) 271, 725, 920, 993, 1119
BstMAI GTCTC 2 cut(s) 129, 579
BstMBI GATC 5 cut(s) 268, 722, 917, 990, 1116
BstMWI GCNNNNNNNGC 5 cut(s) 386, 694, 1154, 1181, 1184
BstNI CCWGG 2 cut(s) 110, 435
BstPAI GACNNNNGTC 1 cut(s) 737
BstSCI CCNGG 2 cut(s) 108, 433
BstSFI CTRYAG 1 cut(s) 369
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 8 cut(s) 361, 364, 436, 458, 1103, 1157, 1171, 1187
BstX2I RGATCY 2 cut(s) 268, 990
BstXI CCANNNNNNTGG 1 cut(s) 89
BstYI RGATCY 2 cut(s) 268, 990
Bsu36I CCTNAGG 1 cut(s) 743
BsuRI GGCC 2 cut(s) 200, 1098
BtgI CCRYGG 1 cut(s) 1127
BtsIMutI CAGTG 1 cut(s) 834
BveI ACCTGC 2 cut(s) 256, 1130
Cac8I GCNNGC 3 cut(s) 756, 1089, 1096
CaiI CAGNNNCTG 1 cut(s) 1181
Cfr13I GGNCC 1 cut(s) 1061
Csp6I GTAC 2 cut(s) 600, 931
CviAII CATG 5 cut(s) 644, 671, 981, 1033, 1084
CviQI GTAC 2 cut(s) 600, 931
DdeI CTNAG 5 cut(s) 57, 195, 294, 619, 743
DpnI GATC 5 cut(s) 270, 724, 919, 992, 1118
DpnII GATC 5 cut(s) 268, 722, 917, 990, 1116
Ecl136II GAGCTC 1 cut(s) 138
Eco147I AGGCCT 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 140
Eco31I GGTCTC 1 cut(s) 579
Eco47I GGWCC 1 cut(s) 1061
Eco53kI GAGCTC 1 cut(s) 138
Eco57I CTGAAG 2 cut(s) 720, 881
Eco81I CCTNAGG 1 cut(s) 743
EcoICRI GAGCTC 1 cut(s) 138
EcoRI GAATTC 2 cut(s) 283, 891
EcoRII CCWGG 2 cut(s) 108, 433
EcoT38I GRGCYC 1 cut(s) 140
FaeI CATG 5 cut(s) 647, 674, 984, 1036, 1087
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 742, 774
FaqI GGGAC 1 cut(s) 1047
FatI CATG 5 cut(s) 643, 670, 980, 1032, 1083
FbaI TGATCA 1 cut(s) 1116
Fnu4HI GCNGC 8 cut(s) 375, 378, 447, 450, 1092, 1146, 1176, 1185
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 8 cut(s) 375, 378, 447, 450, 1092, 1146, 1176, 1185
FspBI CTAG 4 cut(s) 36, 240, 288, 360
GluI GCNGC 8 cut(s) 375, 378, 447, 450, 1092, 1146, 1176, 1185
GsuI CTGGAG 1 cut(s) 65
HaeIII GGCC 2 cut(s) 200, 1098
HapII CCGG 1 cut(s) 74
Hin1II CATG 5 cut(s) 647, 674, 984, 1036, 1087
HincII GTYRAC 2 cut(s) 705, 877
HindII GTYRAC 2 cut(s) 705, 877
HindIII AAGCTT 1 cut(s) 756
HinfI GANTC 3 cut(s) 64, 767, 839
HpaI GTTAAC 1 cut(s) 705
HpaII CCGG 1 cut(s) 74
HphI GGTGA 4 cut(s) 165, 228, 555, 1111
Hpy166II GTNNAC 6 cut(s) 318, 330, 600, 705, 877, 949
Hpy188I TCNGA 5 cut(s) 622, 727, 838, 900, 1015
Hpy188III TCNNGA 4 cut(s) 256, 554, 742, 777
Hpy8I GTNNAC 6 cut(s) 318, 330, 600, 705, 877, 949
HpyAV CCTTC 6 cut(s) 180, 243, 292, 393, 530, 1035
HpyCH4III ACNGT 1 cut(s) 1069
HpyF10VI GCNNNNNNNGC 5 cut(s) 386, 694, 1154, 1181, 1184
HpyF3I CTNAG 5 cut(s) 57, 195, 294, 619, 743
Hsp92II CATG 5 cut(s) 647, 674, 984, 1036, 1087
Ksp22I TGATCA 1 cut(s) 1116
KspAI GTTAAC 1 cut(s) 705
Kzo9I GATC 5 cut(s) 268, 722, 917, 990, 1116
LmnI GCTCC 4 cut(s) 143, 160, 460, 869
Lsp1109I GCAGC 8 cut(s) 361, 364, 436, 458, 1103, 1157, 1171, 1187
LweI GCATC 3 cut(s) 483, 547, 775
MaeI CTAG 4 cut(s) 36, 240, 288, 360
MaeIII GTNAC 1 cut(s) 304
MalI GATC 5 cut(s) 270, 724, 919, 992, 1118
MboI GATC 5 cut(s) 268, 722, 917, 990, 1116
MboII GAAGA 4 cut(s) 525, 662, 887, 1012
MflI RGATCY 2 cut(s) 268, 990
MhlI GDGCHC 3 cut(s) 140, 320, 715
MluCI AATT 6 cut(s) 98, 283, 498, 692, 803, 891
MlyI GAGTC 1 cut(s) 776
MmeI TCCRAC 3 cut(s) 182, 700, 895
MseI TTAA 2 cut(s) 129, 704
MslI CAYNNNNRTG 1 cut(s) 313
MspA1I CMGCKG 2 cut(s) 449, 1094
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 2 cut(s) 110, 435
Mva1269I GAATGC 1 cut(s) 251
MvaI CCWGG 2 cut(s) 110, 435
MwoI GCNNNNNNNGC 5 cut(s) 386, 694, 1154, 1181, 1184
NdeII GATC 5 cut(s) 268, 722, 917, 990, 1116
NlaIII CATG 5 cut(s) 647, 674, 984, 1036, 1087
NlaIV GGNNCC 4 cut(s) 432, 549, 1063, 1133
NmuCI GTSAC 1 cut(s) 304
OliI CACNNNNGTG 1 cut(s) 313
PaqCI CACCTGC 2 cut(s) 256, 1130
PceI AGGCCT 1 cut(s) 200
PcsI WCGNNNNNNNCGW 1 cut(s) 493
PctI GAATGC 1 cut(s) 251
PfeI GAWTC 2 cut(s) 64, 839
PkrI GCNGC 8 cut(s) 376, 379, 448, 451, 1093, 1147, 1177, 1186
PleI GAGTC 1 cut(s) 775
PpsI GAGTC 1 cut(s) 775
PshAI GACNNNNGTC 1 cut(s) 737
PsiI TTATAA 1 cut(s) 1192
Psp124BI GAGCTC 1 cut(s) 140
Psp6I CCWGG 2 cut(s) 108, 433
PspGI CCWGG 2 cut(s) 108, 433
PspN4I GGNNCC 4 cut(s) 432, 549, 1063, 1133
PspPI GGNCC 1 cut(s) 1061
PstNI CAGNNNCTG 1 cut(s) 1181
PsuI RGATCY 2 cut(s) 268, 990
PvuII CAGCTG 2 cut(s) 449, 1094
RsaI GTAC 2 cut(s) 601, 932
RsaNI GTAC 2 cut(s) 600, 931
RseI CAYNNNNRTG 1 cut(s) 313
SacI GAGCTC 1 cut(s) 140
SaqAI TTAA 2 cut(s) 129, 704
SatI GCNGC 8 cut(s) 375, 378, 447, 450, 1092, 1146, 1176, 1185
Sau3AI GATC 5 cut(s) 268, 722, 917, 990, 1116
Sau96I GGNCC 1 cut(s) 1061
ScaI AGTACT 1 cut(s) 932
SchI GAGTC 1 cut(s) 776
ScrFI CCNGG 2 cut(s) 110, 435
SduI GDGCHC 3 cut(s) 140, 320, 715
SfaNI GCATC 3 cut(s) 483, 547, 775
SfcI CTRYAG 1 cut(s) 369
SinI GGWCC 1 cut(s) 1061
SmiMI CAYNNNNRTG 1 cut(s) 313
SmlI CTYRAG 1 cut(s) 714
SmoI CTYRAG 1 cut(s) 714
SpeI ACTAGT 1 cut(s) 35
Sse9I AATT 6 cut(s) 98, 283, 498, 692, 803, 891
SseBI AGGCCT 1 cut(s) 200
SsiI CCGC 1 cut(s) 595
SspMI CTAG 4 cut(s) 36, 240, 288, 360
SstI GAGCTC 1 cut(s) 140
StuI AGGCCT 1 cut(s) 200
StyD4I CCNGG 2 cut(s) 108, 433
TaaI ACNGT 1 cut(s) 1069
TaqI TCGA 1 cut(s) 865
TasI AATT 6 cut(s) 98, 283, 498, 692, 803, 891
TatI WGTACW 2 cut(s) 599, 930
TfiI GAWTC 2 cut(s) 64, 839
Tru1I TTAA 2 cut(s) 129, 704
Tru9I TTAA 2 cut(s) 129, 704
TscAI CASTG 1 cut(s) 834
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 8 cut(s) 374, 377, 446, 449, 1091, 1145, 1175, 1184
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 4 cut(s) 77, 549, 663, 1072
TspRI CASTG 1 cut(s) 834
VneI GTGCAC 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 1061
XapI RAATTY 3 cut(s) 283, 803, 891
XspI CTAG 4 cut(s) 36, 240, 288, 360
ZrmI AGTACT 1 cut(s) 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.