MD10G1225100.v1.1

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012860
Physical Location & Seq
Reverse (-)
32257318 .. 32259876
2559 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1225100.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGTGTTCCTCGAAACCGAAATTGCAACGAAGTGTTAGTGTCTCTGCAATGTCCGCGAAACTGATCAACCAGCGTCCAGTTCTCCAGCCAACCGGCAACCAATTTCCAAGCTTGGAACAAAAAAAGTCTCCTAAGAAAAGCTCCCAAGAAACTAATGTTCCAACTCCTCTTCCTTCACCAGTTCCAACTACTAAAACTAAGGCCTCACTTTCTCCTCCTATCTCTCCCAAGTTGCCTTCACCTCGACAGCCTGCGTTTAAGCGAGGTAAGGATCCCAATCAGCTGAATTCTAGTGCTGAGAAGGTTGTAATGACGCCTCGTTGTGCAACCAAATCCACAAATTTTTTGAAAAAATCGAAGAAATCTAGTGGCGTTGCACCTTCTGCTGAAAGTGTGTTGAAGAATATATCGTCTTTGATAGTAGAGGCACCAGGAAGCATAGCAACTGCAAGGAGGGAGCAAGTAGCAACAATGCAAGAACAGCGAAAGATGCGAATTGCACATTACGGAAGAATAAAGTCTGCCAAGTATGAAGGGAAAGTTGTTCCTCTTGATTCTTCACAACCGAATGATTTCAGTCAAGAGAAAAGGCGATGCAGTTTCATCACCCCTAATTCAGACCCAATATATGTTGCTTACCATGATGAAGAATGGGGAGTTCCCGTCCATGATGACAATATGTTGCTTGAATTGCTACTGTTAACTGGTGCTCAAGTGGGATCAGATTGGACTTCAGTCCTGAGGAGAAGGGAAACATTGAGGGAGGCTTTTTCAGGGTTTGATGCAGATGTTGTGGCTAAATTTAGTGAAAAGAAGATAATATTGTGTTCTGATTCTGGCATAGATATAAGCCTTGTTCGAGGAGCTGTTGACAACGCTAAGAGAATTCTTGAGATAAAGAGGGAAGTGGGATCATTTGACAAGTACTTGTGGGGATTTGTGAATCACAAACCAATATCTACCCAATACAAGTCATGCCACAAGATACCTGTGAAGAACTCCAAGTCAGAGGCCATCAGCAAAGACATGGTTAGAAGGGGTTTTAGGCACGTCGGTCCCACTGTGATTCACTCCTTCATGCAAGCAGCTGGCCTCACTAACGACCACTTGATCACCTGCCCAAGGCACCTCCACTGCATCCCTTCACCATCATCACCCATGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

389

Amino Acids

42.91

Weight (kDa)

9.86

Isoelectric Point (pI)

54.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 205 - 376 1.8e-61 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012860)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44680
fragaria_vesca FvH4_3g13300
malus_domestica MD05G1243000.v1.1 MD10G1225100.v1.1
prunus_persica Prupe.4G120400_v2.0.a1
pyrus_communis pycom05g21960 pycom10g18890
rosa_chinensis RchiOBHm_Chr5g0021771
rosa_laevigata RLG00000032626
rosa_multiflora Rmu_ssc0000233.1_g000002
rosa_roxburghii Rroxscaffold_1G00056940
rosa_rugosa Rorug05G0069300 Rorug05G0069400
rosa_samantha Rh5AG157900 Rh5BG157400 Rh5CG171600 Rh5DG157700
rosa_wichuraiana Rw5G014060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1124
Acc36I ACCTGC 1 cut(s) 1124
AccB1I GGYRCC 2 cut(s) 427, 1125
AccII CGCG 1 cut(s) 56
AciI CCGC 1 cut(s) 54
AclWI GGATC 4 cut(s) 266, 279, 727, 919
AcsI RAATTY 4 cut(s) 286, 340, 800, 885
AcuI CTGAAG 1 cut(s) 717
AcyI GRCGYC 1 cut(s) 314
AfaI GTAC 1 cut(s) 926
AfiI CCNNNNNNNGG 1 cut(s) 1122
AgsI TTSAA 3 cut(s) 349, 400, 689
AjiI CACGTC 1 cut(s) 1051
AjnI CCWGG 1 cut(s) 430
AluBI AGCT 5 cut(s) 111, 141, 283, 866, 1088
AluI AGCT 5 cut(s) 111, 141, 283, 866, 1088
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 2 cut(s) 46, 132
AlwI GGATC 4 cut(s) 266, 279, 727, 919
AoxI GGCC 3 cut(s) 201, 1011, 1090
ApeKI GCWGC 1 cut(s) 1085
ApoI RAATTY 4 cut(s) 286, 340, 800, 885
Asp700I GAANNNNTTC 1 cut(s) 572
AspS9I GGNCC 1 cut(s) 1055
AsuHPI GGTGA 6 cut(s) 168, 231, 598, 1105, 1137, 1146
AvaII GGWCC 1 cut(s) 1055
AxyI CCTNAGG 1 cut(s) 740
BamHI GGATCC 1 cut(s) 271
BanI GGYRCC 2 cut(s) 427, 1125
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 1 cut(s) 1097
BccI CCATC 2 cut(s) 1022, 1156
BciT130I CCWGG 1 cut(s) 432
BclI TGATCA 2 cut(s) 63, 1110
BcoDI GTCTC 2 cut(s) 46, 132
BfaI CTAG 2 cut(s) 291, 366
BfuAI ACCTGC 1 cut(s) 1124
BisI GCNGC 1 cut(s) 1086
BlsI GCNGC 1 cut(s) 1087
BmcAI AGTACT 1 cut(s) 926
Bme1390I CCNGG 1 cut(s) 432
Bme18I GGWCC 1 cut(s) 1055
BmgBI CACGTC 1 cut(s) 1051
BmgT120I GGNCC 1 cut(s) 1055
BmiI GGNNCC 4 cut(s) 273, 429, 1057, 1127
BmrFI CCNGG 1 cut(s) 432
BmsI GCATC 4 cut(s) 480, 584, 772, 1146
BoxI GACNNNNGTC 1 cut(s) 734
BpmI CTGGAG 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 696, 911
BsaBI GATNNNNATC 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 1121
BsaXI ACNNNNNCTCC 2 cut(s) 648, 678
Bsc4I CCNNNNNNNGG 1 cut(s) 1122
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 3 cut(s) 77, 179, 709
Bse21I CCTNAGG 1 cut(s) 740
Bse3DI GCAATG 1 cut(s) 54
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 1121
BseGI GGATG 1 cut(s) 1137
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 1122
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 2 cut(s) 288, 731
BseNI ACTGG 3 cut(s) 77, 179, 709
BseRI GAGGAG 4 cut(s) 156, 204, 757, 876
BseXI GCAGC 1 cut(s) 1097
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 3 cut(s) 203, 1013, 1092
BshNI GGYRCC 2 cut(s) 427, 1125
BsiHKAI GWGCWC 1 cut(s) 712
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 1041
BslI CCNNNNNNNGG 1 cut(s) 1122
BsmAI GTCTC 2 cut(s) 46, 132
BsmFI GGGAC 1 cut(s) 1041
BsnI GGCC 3 cut(s) 203, 1013, 1092
Bsp1286I GDGCHC 1 cut(s) 712
Bsp143I GATC 5 cut(s) 63, 271, 719, 911, 1110
BspACI CCGC 1 cut(s) 54
BspANI GGCC 3 cut(s) 203, 1013, 1092
BspCNI CTCAG 2 cut(s) 289, 732
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 4 cut(s) 273, 429, 1057, 1127
BspMI ACCTGC 1 cut(s) 1124
BspPI GGATC 4 cut(s) 266, 279, 727, 919
BspT107I GGYRCC 2 cut(s) 427, 1125
BsrDI GCAATG 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 3 cut(s) 77, 179, 709
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 1121
BssMI GATC 5 cut(s) 63, 271, 719, 911, 1110
BssNI GRCGYC 1 cut(s) 314
BssT1I CCWWGG 1 cut(s) 1121
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 2 cut(s) 699, 1063
Bst6I CTCTTC 1 cut(s) 174
BstACI GRCGYC 1 cut(s) 314
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 3 cut(s) 252, 1083, 1090
BstDEI CTNAG 5 cut(s) 132, 198, 297, 740, 879
BstENI CCTNNNNNAGG 1 cut(s) 1120
BstF5I GGATG 1 cut(s) 1137
BstFNI CGCG 1 cut(s) 56
BstKTI GATC 5 cut(s) 66, 274, 722, 914, 1113
BstMAI GTCTC 2 cut(s) 46, 132
BstMBI GATC 5 cut(s) 63, 271, 719, 911, 1110
BstMWI GCNNNNNNNGC 5 cut(s) 53, 383, 481, 490, 691
BstNI CCWGG 1 cut(s) 432
BstPAI GACNNNNGTC 1 cut(s) 734
BstSCI CCNGG 1 cut(s) 430
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 1097
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
Bsu36I CCTNAGG 1 cut(s) 740
BsuRI GGCC 3 cut(s) 203, 1013, 1092
BtgZI GCGATG 1 cut(s) 607
BtrI CACGTC 1 cut(s) 1051
BtsCI GGATG 1 cut(s) 1137
BtsI GCAGTG 1 cut(s) 1132
BtsIMutI CAGTG 2 cut(s) 1059, 1132
BveI ACCTGC 1 cut(s) 1124
Cac8I GCNNGC 3 cut(s) 252, 1083, 1090
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 1055
CseI GACGC 2 cut(s) 62, 322
Csp6I GTAC 1 cut(s) 925
CviAII CATG 6 cut(s) 641, 668, 975, 1027, 1078, 1159
CviQI GTAC 1 cut(s) 925
DdeI CTNAG 5 cut(s) 132, 198, 297, 740, 879
DpnI GATC 5 cut(s) 65, 273, 721, 913, 1112
DpnII GATC 5 cut(s) 63, 271, 719, 911, 1110
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 1121
Eco147I AGGCCT 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 1055
Eco57I CTGAAG 1 cut(s) 717
Eco81I CCTNAGG 1 cut(s) 740
EcoNI CCTNNNNNAGG 1 cut(s) 1120
EcoRI GAATTC 2 cut(s) 286, 885
EcoRII CCWGG 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 1121
ErhI CCWWGG 1 cut(s) 1121
FaeI CATG 6 cut(s) 644, 671, 978, 1030, 1081, 1162
FaqI GGGAC 1 cut(s) 1041
FatI CATG 6 cut(s) 640, 667, 974, 1026, 1077, 1158
FbaI TGATCA 2 cut(s) 63, 1110
Fnu4HI GCNGC 1 cut(s) 1086
FokI GGATG 1 cut(s) 1124
Fsp4HI GCNGC 1 cut(s) 1086
FspBI CTAG 2 cut(s) 291, 366
GluI GCNGC 1 cut(s) 1086
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 3 cut(s) 203, 1013, 1092
HapII CCGG 1 cut(s) 93
HgaI GACGC 2 cut(s) 62, 322
Hin1I GRCGYC 1 cut(s) 314
Hin1II CATG 6 cut(s) 644, 671, 978, 1030, 1081, 1162
HincII GTYRAC 2 cut(s) 702, 871
HindII GTYRAC 2 cut(s) 702, 871
HindIII AAGCTT 1 cut(s) 109
HinfI GANTC 4 cut(s) 554, 833, 943, 1066
HpaI GTTAAC 1 cut(s) 702
HpaII CCGG 1 cut(s) 93
HphI GGTGA 6 cut(s) 168, 231, 598, 1105, 1137, 1146
Hpy166II GTNNAC 2 cut(s) 702, 871
Hpy188I TCNGA 4 cut(s) 619, 724, 832, 1009
Hpy188III TCNNGA 4 cut(s) 551, 581, 739, 890
Hpy8I GTNNAC 2 cut(s) 702, 871
Hpy99I CGWCG 1 cut(s) 1055
HpyAV CCTTC 9 cut(s) 183, 246, 295, 390, 527, 741, 1029, 1084, 1152
HpyCH4III ACNGT 2 cut(s) 699, 1063
HpyCH4IV ACGT 1 cut(s) 1050
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 383, 481, 490, 691
HpyF3I CTNAG 5 cut(s) 132, 198, 297, 740, 879
HpySE526I ACGT 1 cut(s) 1050
Hsp92I GRCGYC 1 cut(s) 314
Hsp92II CATG 6 cut(s) 644, 671, 978, 1030, 1081, 1162
Ksp22I TGATCA 2 cut(s) 63, 1110
KspAI GTTAAC 1 cut(s) 702
Kzo9I GATC 5 cut(s) 63, 271, 719, 911, 1110
LmnI GCTCC 3 cut(s) 146, 457, 863
Lsp1109I GCAGC 1 cut(s) 1097
LweI GCATC 4 cut(s) 480, 584, 772, 1146
MaeI CTAG 2 cut(s) 291, 366
MaeII ACGT 1 cut(s) 1050
MalI GATC 5 cut(s) 65, 273, 721, 913, 1112
MboI GATC 5 cut(s) 63, 271, 719, 911, 1110
MboII GAAGA 8 cut(s) 161, 370, 412, 522, 549, 659, 826, 1006
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 1 cut(s) 712
MluCI AATT 9 cut(s) 20, 101, 286, 340, 495, 613, 689, 800, 885
MmeI TCCRAC 2 cut(s) 185, 209
MroXI GAANNNNTTC 1 cut(s) 572
MseI TTAA 2 cut(s) 258, 701
MspA1I CMGCKG 2 cut(s) 283, 1088
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 432
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 5 cut(s) 53, 383, 481, 490, 691
NdeII GATC 5 cut(s) 63, 271, 719, 911, 1110
NlaIII CATG 6 cut(s) 644, 671, 978, 1030, 1081, 1162
NlaIV GGNNCC 4 cut(s) 273, 429, 1057, 1127
PaqCI CACCTGC 1 cut(s) 1124
PceI AGGCCT 1 cut(s) 203
PdmI GAANNNNTTC 1 cut(s) 572
PfeI GAWTC 4 cut(s) 554, 833, 943, 1066
PkrI GCNGC 1 cut(s) 1087
PshAI GACNNNNGTC 1 cut(s) 734
Psp6I CCWGG 1 cut(s) 430
PspGI CCWGG 1 cut(s) 430
PspN4I GGNNCC 4 cut(s) 273, 429, 1057, 1127
PspPI GGNCC 1 cut(s) 1055
PsuI RGATCY 1 cut(s) 271
PvuII CAGCTG 2 cut(s) 283, 1088
RsaI GTAC 1 cut(s) 926
RsaNI GTAC 1 cut(s) 925
SaqAI TTAA 2 cut(s) 258, 701
SatI GCNGC 1 cut(s) 1086
Sau3AI GATC 5 cut(s) 63, 271, 719, 911, 1110
Sau96I GGNCC 1 cut(s) 1055
ScaI AGTACT 1 cut(s) 926
ScrFI CCNGG 1 cut(s) 432
SduI GDGCHC 1 cut(s) 712
SfaNI GCATC 4 cut(s) 480, 584, 772, 1146
SinI GGWCC 1 cut(s) 1055
SmlI CTYRAG 2 cut(s) 711, 890
SmoI CTYRAG 2 cut(s) 711, 890
Sse9I AATT 9 cut(s) 20, 101, 286, 340, 495, 613, 689, 800, 885
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 1 cut(s) 54
SspI AATATT 1 cut(s) 822
SspMI CTAG 2 cut(s) 291, 366
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 430
StyI CCWWGG 1 cut(s) 1121
TaaI ACNGT 2 cut(s) 699, 1063
TaiI ACGT 1 cut(s) 1053
TaqI TCGA 4 cut(s) 11, 244, 356, 859
TaqII GACCGA 1 cut(s) 1043
TasI AATT 9 cut(s) 20, 101, 286, 340, 495, 613, 689, 800, 885
TatI WGTACW 1 cut(s) 924
TfiI GAWTC 4 cut(s) 554, 833, 943, 1066
Tru1I TTAA 2 cut(s) 258, 701
Tru9I TTAA 2 cut(s) 258, 701
TscAI CASTG 2 cut(s) 1066, 1139
TseI GCWGC 1 cut(s) 1085
TspDTI ATGAA 4 cut(s) 546, 592, 660, 1066
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 2 cut(s) 1066, 1139
VpaK11BI GGWCC 1 cut(s) 1055
XagI CCTNNNNNAGG 1 cut(s) 1120
XapI RAATTY 4 cut(s) 286, 340, 800, 885
XmnI GAANNNNTTC 1 cut(s) 572
XspI CTAG 2 cut(s) 291, 366
ZrmI AGTACT 1 cut(s) 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.