pycom05g21960

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012860
Physical Location & Seq
Reverse (-)
24503349 .. 24505396
2048 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21960.4

Sequence Viewer

Length: 1179 bp
ATGTGTTCCGCGAAACCAAAATTGCAACGAAGTGCTAGTGTCTCTGCAACGTCAGGGAAACTAATCAACCGGCGTCCGGTTCTCCAGCCAACCGGCAATCAATTTCCCACCTTGGAACAAAAAAAGTCTCCTAAAAAAAGCTCCCAAGAAACTAATGTTCCAATTCCTCTTCCTTCACCACTTCCAACTAATAAAACTAAGGAAACTAAGGCCTCACTTTCTCCTCCTATCTCTCCCAAGTTGCCTTCACCTCGACAGCCTGCGTTTAAGCGAGGTAAAGATCCCAATGAGCTGAATTCTAGTGCTGAGAAGGTTGTAGTGACGCCTCGTTGCTCAACCAAATCGACAAGTTCGGTGAAGAAGTCGAAGAAATCTAGTGGTGTTGCACCTTCTGCCGAAAGTGTTTTGAAGAATGTGTCGTCTTTGATAGTAGAGGCACCAGGAAGCATAGCGGCAGCAAGGAGGGAGCAAGTAGCAACAATGCAAGAACAGCGAAAGATGCGAATTGCGCATTATGGAAGAGCAAAGTCTGCCAAGTATGAAGGGAAAGTTGTTCCTCTTGATTCTTCAGCTACAAATGATTTCGGTCAAGAGCAAAGGAGATGCAGTTTCATCACCCCCAAGTCAGACCCAATATATGTTGCTTACCATGATGAAGAATGGGGAGTTCCTGTCCATGATGACAATATGTTGCTTGAATTGCTACTATTAACTGGTGCTCAAGTGGGATCGGATTGGACTTCAGTCCTAAGGAAAAGGGAAGCATTGAGGGAGGCTTTTTCAGGGTTTGATGCAGATGTTGTGGCTAAATTTAGTGAAAAAAGAATAACATCAGTGAGTGCTGATTCTGGCATAGATATTAGCCTTGTTCGAGGACTTGTTGACAATGCTAAGAGAATTCTTGAGGTAAAGAGGGAAATGGGATCATTTGACAATTACTTGTGGGGATTTGTGAATCACAAGCCAATATGTACCCAATACAAGTCATGCCACAAGATTCCTGTTAAGAACTCCAAGTCAGAGGCCATCAGCAAAGACATGGTTAGAAGGGGTTTTAGGCATGTTGGTCCCACTGTAATTCACTTCTTCATGCAAGCAGCTGGCCTCACCAATGACCACTTGATCACCTGCCCAAGACACCTCCACTGCATCCCTTCACCATCATCACCCATGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

393

Amino Acids

43.11

Weight (kDa)

9.82

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012860)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44680
fragaria_vesca FvH4_3g13300
malus_domestica MD05G1243000.v1.1 MD10G1225100.v1.1
prunus_persica Prupe.4G120400_v2.0.a1
pyrus_communis pycom05g21960 pycom10g18890
rosa_chinensis RchiOBHm_Chr5g0021771
rosa_laevigata RLG00000032626
rosa_multiflora Rmu_ssc0000233.1_g000002
rosa_roxburghii Rroxscaffold_1G00056940
rosa_rugosa Rorug05G0069300 Rorug05G0069400
rosa_samantha Rh5AG157900 Rh5BG157400 Rh5CG171600 Rh5DG157700
rosa_wichuraiana Rw5G014060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1136
Acc16I TGCGCA 1 cut(s) 510
Acc36I ACCTGC 1 cut(s) 1136
AccB1I GGYRCC 1 cut(s) 436
AccII CGCG 1 cut(s) 11
AciI CCGC 2 cut(s) 9, 452
AclWI GGATC 3 cut(s) 275, 736, 931
AcsI RAATTY 3 cut(s) 295, 809, 897
AcuI CTGAAG 2 cut(s) 552, 726
AcyI GRCGYC 2 cut(s) 73, 323
AfaI GTAC 1 cut(s) 973
AfiI CCNNNNNNNGG 1 cut(s) 76
AgsI TTSAA 2 cut(s) 409, 698
AjnI CCWGG 1 cut(s) 439
AluBI AGCT 4 cut(s) 141, 292, 572, 1100
AluI AGCT 4 cut(s) 141, 292, 572, 1100
Alw21I GWGCWC 1 cut(s) 721
Alw26I GTCTC 2 cut(s) 46, 132
AlwI GGATC 3 cut(s) 275, 736, 931
AoxI GGCC 3 cut(s) 210, 1023, 1102
ApeKI GCWGC 2 cut(s) 455, 1097
ApoI RAATTY 3 cut(s) 295, 809, 897
AspLEI GCGC 1 cut(s) 511
AspS9I GGNCC 1 cut(s) 1067
AsuHPI GGTGA 8 cut(s) 168, 240, 367, 607, 1099, 1117, 1149, 1158
AvaII GGWCC 1 cut(s) 1067
AxyI CCTNAGG 1 cut(s) 749
BanI GGYRCC 1 cut(s) 436
Bbv12I GWGCWC 1 cut(s) 721
BbvI GCAGC 2 cut(s) 467, 1109
BccI CCATC 2 cut(s) 1034, 1168
BciT130I CCWGG 1 cut(s) 441
BclI TGATCA 1 cut(s) 1122
BcoDI GTCTC 2 cut(s) 46, 132
BfaI CTAG 3 cut(s) 36, 300, 375
BfuAI ACCTGC 1 cut(s) 1136
BisI GCNGC 3 cut(s) 453, 456, 1098
BlsI GCNGC 3 cut(s) 454, 457, 1099
Bme1390I CCNGG 1 cut(s) 441
Bme18I GGWCC 1 cut(s) 1067
BmgT120I GGNCC 1 cut(s) 1067
BmiI GGNNCC 2 cut(s) 438, 1069
BmrFI CCNGG 1 cut(s) 441
BmsI GCATC 4 cut(s) 489, 593, 781, 1158
BoxI GACNNNNGTC 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 705, 923
BsaHI GRCGYC 2 cut(s) 73, 323
BsaJI CCNNGG 1 cut(s) 111
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 4 cut(s) 592, 622, 657, 687
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse118I RCCGGY 2 cut(s) 69, 92
Bse1I ACTGG 1 cut(s) 718
Bse21I CCTNAGG 1 cut(s) 749
BseBI CCWGG 1 cut(s) 441
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 1 cut(s) 1149
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 1 cut(s) 718
BseRI GAGGAG 1 cut(s) 213
BseXI GCAGC 2 cut(s) 467, 1109
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 3 cut(s) 212, 1025, 1104
BshNI GGYRCC 1 cut(s) 436
BsiHKAI GWGCWC 1 cut(s) 721
BsiSI CCGG 3 cut(s) 70, 77, 93
BslFI GGGAC 1 cut(s) 1053
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 2 cut(s) 46, 132
BsmFI GGGAC 1 cut(s) 1053
BsnI GGCC 3 cut(s) 212, 1025, 1104
Bsp1286I GDGCHC 1 cut(s) 721
Bsp143I GATC 4 cut(s) 280, 728, 923, 1122
BspACI CCGC 2 cut(s) 9, 452
BspANI GGCC 3 cut(s) 212, 1025, 1104
BspCNI CTCAG 1 cut(s) 298
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 2 cut(s) 438, 1069
BspMI ACCTGC 1 cut(s) 1136
BspPI GGATC 3 cut(s) 275, 736, 931
BspQI GCTCTTC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 436
BsrFI RCCGGY 2 cut(s) 69, 92
BsrI ACTGG 1 cut(s) 718
BssAI RCCGGY 2 cut(s) 69, 92
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 4 cut(s) 280, 728, 923, 1122
BssNI GRCGYC 2 cut(s) 73, 323
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 441
Bst4CI ACNGT 1 cut(s) 1075
Bst6I CTCTTC 2 cut(s) 174, 514
BstACI GRCGYC 2 cut(s) 73, 323
BstAPI GCANNNNNTGC 2 cut(s) 392, 530
BstC8I GCNNGC 3 cut(s) 261, 1095, 1102
BstDEI CTNAG 5 cut(s) 198, 207, 306, 749, 891
BstF5I GGATG 1 cut(s) 1149
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 511
BstKTI GATC 4 cut(s) 283, 731, 926, 1125
BstMAI GTCTC 2 cut(s) 46, 132
BstMBI GATC 4 cut(s) 280, 728, 923, 1122
BstMWI GCNNNNNNNGC 6 cut(s) 392, 490, 499, 508, 530, 700
BstNI CCWGG 1 cut(s) 441
BstNSI RCATGY 1 cut(s) 1064
BstPAI GACNNNNGTC 1 cut(s) 743
BstSCI CCNGG 1 cut(s) 439
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 467, 1109
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
Bsu36I CCTNAGG 1 cut(s) 749
BsuRI GGCC 3 cut(s) 212, 1025, 1104
BtsCI GGATG 1 cut(s) 1149
BtsI GCAGTG 1 cut(s) 1144
BtsIMutI CAGTG 3 cut(s) 840, 1071, 1144
BveI ACCTGC 1 cut(s) 1136
Cac8I GCNNGC 3 cut(s) 261, 1095, 1102
CfoI GCGC 1 cut(s) 511
Cfr10I RCCGGY 2 cut(s) 69, 92
Cfr13I GGNCC 1 cut(s) 1067
CseI GACGC 2 cut(s) 62, 331
Csp6I GTAC 1 cut(s) 972
CviAII CATG 7 cut(s) 650, 677, 987, 1039, 1061, 1090, 1171
CviQI GTAC 1 cut(s) 972
DdeI CTNAG 5 cut(s) 198, 207, 306, 749, 891
DpnI GATC 4 cut(s) 282, 730, 925, 1124
DpnII GATC 4 cut(s) 280, 728, 923, 1122
Eam1104I CTCTTC 2 cut(s) 174, 514
EarI CTCTTC 2 cut(s) 174, 514
Eco130I CCWWGG 1 cut(s) 111
Eco147I AGGCCT 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 1067
Eco57I CTGAAG 2 cut(s) 552, 726
Eco81I CCTNAGG 1 cut(s) 749
EcoRI GAATTC 2 cut(s) 295, 897
EcoRII CCWGG 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 7 cut(s) 653, 680, 990, 1042, 1064, 1093, 1174
FaqI GGGAC 1 cut(s) 1053
FatI CATG 7 cut(s) 649, 676, 986, 1038, 1060, 1089, 1170
FbaI TGATCA 1 cut(s) 1122
Fnu4HI GCNGC 3 cut(s) 453, 456, 1098
FokI GGATG 1 cut(s) 1136
Fsp4HI GCNGC 3 cut(s) 453, 456, 1098
FspBI CTAG 3 cut(s) 36, 300, 375
FspI TGCGCA 1 cut(s) 510
GlaI GCGC 1 cut(s) 510
GluI GCNGC 3 cut(s) 453, 456, 1098
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 3 cut(s) 212, 1025, 1104
HapII CCGG 3 cut(s) 70, 77, 93
HgaI GACGC 2 cut(s) 62, 331
HhaI GCGC 1 cut(s) 511
Hin1I GRCGYC 2 cut(s) 73, 323
Hin1II CATG 7 cut(s) 653, 680, 990, 1042, 1064, 1093, 1174
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HinfI GANTC 4 cut(s) 563, 845, 955, 997
HpaII CCGG 3 cut(s) 70, 77, 93
HphI GGTGA 8 cut(s) 168, 240, 367, 607, 1099, 1117, 1149, 1158
Hpy166II GTNNAC 1 cut(s) 883
Hpy188I TCNGA 3 cut(s) 628, 733, 1021
Hpy188III TCNNGA 3 cut(s) 560, 590, 902
Hpy8I GTNNAC 1 cut(s) 883
HpyAV CCTTC 7 cut(s) 183, 255, 304, 399, 536, 1041, 1164
HpyCH4III ACNGT 1 cut(s) 1075
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 8 cut(s) 25, 47, 386, 484, 606, 794, 1093, 1149
HpyF10VI GCNNNNNNNGC 6 cut(s) 392, 490, 499, 508, 530, 700
HpyF3I CTNAG 5 cut(s) 198, 207, 306, 749, 891
HpySE526I ACGT 1 cut(s) 50
Hsp92I GRCGYC 2 cut(s) 73, 323
Hsp92II CATG 7 cut(s) 653, 680, 990, 1042, 1064, 1093, 1174
HspAI GCGC 1 cut(s) 509
Ksp22I TGATCA 1 cut(s) 1122
Kzo9I GATC 4 cut(s) 280, 728, 923, 1122
LguI GCTCTTC 1 cut(s) 514
LmnI GCTCC 2 cut(s) 146, 466
Lsp1109I GCAGC 2 cut(s) 467, 1109
LweI GCATC 4 cut(s) 489, 593, 781, 1158
MaeI CTAG 3 cut(s) 36, 300, 375
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 319
MalI GATC 4 cut(s) 282, 730, 925, 1124
MboI GATC 4 cut(s) 280, 728, 923, 1122
MboII GAAGA 8 cut(s) 161, 370, 379, 421, 531, 558, 668, 1078
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 721
MmeI TCCRAC 1 cut(s) 209
MseI TTAA 3 cut(s) 267, 710, 1005
MspA1I CMGCKG 1 cut(s) 1100
MspI CCGG 3 cut(s) 70, 77, 93
MspR9I CCNGG 1 cut(s) 441
MvaI CCWGG 1 cut(s) 441
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 6 cut(s) 392, 490, 499, 508, 530, 700
NdeII GATC 4 cut(s) 280, 728, 923, 1122
NlaIII CATG 7 cut(s) 653, 680, 990, 1042, 1064, 1093, 1174
NlaIV GGNNCC 2 cut(s) 438, 1069
NmuCI GTSAC 1 cut(s) 319
NsbI TGCGCA 1 cut(s) 510
NspI RCATGY 1 cut(s) 1064
PaqCI CACCTGC 1 cut(s) 1136
PceI AGGCCT 1 cut(s) 212
PciSI GCTCTTC 1 cut(s) 514
PfeI GAWTC 4 cut(s) 563, 845, 955, 997
PkrI GCNGC 3 cut(s) 454, 457, 1099
PshAI GACNNNNGTC 1 cut(s) 743
Psp6I CCWGG 1 cut(s) 439
PspGI CCWGG 1 cut(s) 439
PspN4I GGNNCC 2 cut(s) 438, 1069
PspPI GGNCC 1 cut(s) 1067
PsuI RGATCY 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 1100
RsaI GTAC 1 cut(s) 973
RsaNI GTAC 1 cut(s) 972
SapI GCTCTTC 1 cut(s) 514
SaqAI TTAA 3 cut(s) 267, 710, 1005
SatI GCNGC 3 cut(s) 453, 456, 1098
Sau3AI GATC 4 cut(s) 280, 728, 923, 1122
Sau96I GGNCC 1 cut(s) 1067
ScrFI CCNGG 1 cut(s) 441
SduI GDGCHC 1 cut(s) 721
SfaNI GCATC 4 cut(s) 489, 593, 781, 1158
SinI GGWCC 1 cut(s) 1067
SmlI CTYRAG 2 cut(s) 720, 902
SmoI CTYRAG 2 cut(s) 720, 902
SseBI AGGCCT 1 cut(s) 212
SsiI CCGC 2 cut(s) 9, 452
SspMI CTAG 3 cut(s) 36, 300, 375
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 439
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 1075
TaiI ACGT 1 cut(s) 53
TaqI TCGA 4 cut(s) 253, 344, 365, 871
TaqII GACCGA 1 cut(s) 575
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 4 cut(s) 563, 845, 955, 997
Tru1I TTAA 3 cut(s) 267, 710, 1005
Tru9I TTAA 3 cut(s) 267, 710, 1005
TscAI CASTG 3 cut(s) 840, 1078, 1151
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 2 cut(s) 455, 1097
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 4 cut(s) 555, 601, 669, 1078
TspRI CASTG 3 cut(s) 840, 1078, 1151
VpaK11BI GGWCC 1 cut(s) 1067
XapI RAATTY 3 cut(s) 295, 809, 897
XceI RCATGY 1 cut(s) 1064
XspI CTAG 3 cut(s) 36, 300, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.