AT5G45610

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002469
Physical Location & Seq
Reverse (-)
18496228 .. 18499888
3661 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45610.2

Sequence Viewer

Length: 1941 bp
ATGAGTGGAAATGATGAAGAATTTAACGACGAATTTCTCCTCGCCATCGATTCAATCGAAACCACCCTGAAAAAAGCCGATATGTATCGCCCCCTACCTCCTCCGTATCTTCCAACGTTTCTACCGGCTCCTCCGCCATCGACGAAGATTTCATCGTCTTTATCTCACCCTATGCAGTTACAATCCTCAGCGGGACAACAACGGAAACAAATTCAAGTTCCTGATCCTTTCTTAAGCTATTCTCCGCCGAGAGAACTCTCTCAAAGAGTAGTAAGCGGTTTCAATGACGCGTTAATGGATTACTCTAACTCTACTGTTGTTACTGCTGCTAAACCTATCTCTCCGACTACTTCGAATCGTCGCTGTGATAGTGAGAAAGATCTCGAAATTGATCGCTTAAAGAAGGAGCTGGAACGTGTCTCGAAGCAATTGCTGGATGTGGAGCAAGAATGTTCTCAGCTTAAGAAGGGGAAAAGCAAAGAAACAGAGTCTAGAAACTTGTGTGCTGATGATAATAGAGGGCAATGTTCTACGGTTCATGCTTCTAAGAGGATAGACTTGGAACCAGATGTCGCAACTTCTTCAGTAAATCATCGAGAAAACGACTCTAGGATGGCGTTAGATGATAAAAGAAGCTTCAAGACCACTGGTGTTCAGGCTGATGTGGCCAATCATTCTGACCTTTCAAAGAAACTGCTAGACATCTGGCGCACCTCAAATTATCAAGATCCGAGGAAAAATCTAATCTCAGAGTTGCTACTGGCTTGTTCAACAGATCTCCAGATTCTCTTTAGTTTTATGAAAATCAGTACGCCTCCTCAAGAACTTAACAAACAGGAAGCCAAAACCTCGTCAGATAGACAATCTTCTAAAGCCTTAGAATCTGAGAAAGTGTATCAATTATACTCTGCAGTAACAAAGATCAGCTATGGGTTTGTAAATTTGAAGACCTTGGTTGAACCACTACTTGATCTTTGTAAAGCGGAAACTGCCGTTCTTGTTCATAGATCTCTGCGTGTACTACATGTGCTGTTGGAACATATATGTGGTGATGAAAAGAGATTTGAAGCCAGTTGGGATGCAAACTGGCATTCTCTGTTTAAATTGATGAATCAAATTGCTAGTAAAAGAACCGAACAGGATGTGAAACAGGAAGCATTGTCGATAATGAACATAATCGTGATGAGCACCGATGCTTATACTGCTAGAGAAAGTTTTGTCTCCAAGGAAGTGTTTGAAAGCATTTCATTACTTTTAAGAAAAGAAGGTGGCTTACATGTACGGAAAGAAGCTATTCATCTTTTTTACTTGCTACTCAACTGTCCTAAACTATACGATACATTTGATTCTCTCCATGAGGAAAAGAACTCCTCTGATACTGAAAACGATAGTGAAGGAAATTTCTTTGCTTTGGAAGCATTTGGTAAGATCTTTGAAGGCTTAGCAGATTGTTTGACTTCTCCCAGAAAGACATCAGAGGATCTGGAACTGTGTAGGAATGTAATAATGATCTTGGCTCTAGCAGCTTCTTCAGGAAACTCTGGCTATGAACTACTCTCAAGTCACAAGCTACCTCAAGACAGCAGCTTCTTAATGCTGATACTTCATCTTTTAGTTGCTGAAATAGACTCGGAATCAACAGAATTTCATCCAAAAGCGGAAATCTTCAAAGCAAGAACACTATTGATGAGAGAGATATTGATATTACTAAACAGACTTGTCTCTGGCTTGTCAAGCTCTGCTACTATTCTAAAGGAACTAACAACGAGCAGAGACATGGCGAGTCTAACGGTTGATGCAGCCACAAGATTATCCCGTAAAAGAAACTTACTTGGAAAACCTGAAAGCAGTGTTGAGAGAATGAGGAATACCGAAATCATGGATTTAGCCCGCATTTTCAAGAAACGGGTTTTCGCCTTCTTAGGTGACAATACTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

646

Amino Acids

72.81

Weight (kDa)

6.05

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 290
AciI CCGC 7 cut(s) 134, 191, 245, 276, 983, 1658, 1891
AclI AACGTT 1 cut(s) 116
AclWI GGATC 3 cut(s) 218, 722, 1488
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 6 cut(s) 20, 32, 210, 940, 1399, 1643
AcuI CTGAAG 2 cut(s) 567, 1515
AfaI GTAC 3 cut(s) 811, 1020, 1281
AflII CTTAAG 2 cut(s) 232, 461
AflIII ACRYGT 4 cut(s) 288, 415, 1024, 1276
Alw21I GWGCWC 1 cut(s) 1190
Alw26I GTCTC 4 cut(s) 424, 1225, 1726, 1767
AlwI GGATC 3 cut(s) 218, 722, 1488
AoxI GGCC 1 cut(s) 666
ApeKI GCWGC 4 cut(s) 326, 1523, 1584, 1799
ApoI RAATTY 6 cut(s) 20, 32, 210, 940, 1399, 1643
Asp700I GAANNNNTTC 1 cut(s) 1293
AspLEI GCGC 1 cut(s) 711
AsuHPI GGTGA 3 cut(s) 158, 1061, 1937
AsuII TTCGAA 1 cut(s) 353
BalI TGGCCA 1 cut(s) 668
BarI GAAGNNNNNNTAC 2 cut(s) 1257, 1289
BbsI GAAGAC 1 cut(s) 953
Bbv12I GWGCWC 1 cut(s) 1190
BbvCI CCTCAGC 1 cut(s) 187
BbvI GCAGC 4 cut(s) 313, 1535, 1596, 1811
BccI CCATC 3 cut(s) 53, 145, 607
BceAI ACGGC 1 cut(s) 977
BcgI CGANNNNNNTGC 2 cut(s) 412, 446
BcoDI GTCTC 4 cut(s) 424, 1225, 1726, 1767
BfaI CTAG 6 cut(s) 492, 609, 698, 1122, 1206, 1520
BfmI CTRYAG 1 cut(s) 909
BfrI CTTAAG 2 cut(s) 232, 461
BglII AGATCT 4 cut(s) 379, 775, 1007, 1428
BisI GCNGC 4 cut(s) 327, 1524, 1585, 1800
BlpI GCTNAGC 1 cut(s) 1441
BlsI GCNGC 4 cut(s) 328, 1525, 1586, 1801
BmiI GGNNCC 2 cut(s) 129, 564
BmsI GCATC 3 cut(s) 1069, 1183, 1786
BpiI GAAGAC 1 cut(s) 953
BpmI CTGGAG 1 cut(s) 764
Bpu10I CCTNAGC 1 cut(s) 187
Bpu1102I GCTNAGC 1 cut(s) 1441
Bpu14I TTCGAA 1 cut(s) 353
BpuEI CTTGAG 3 cut(s) 804, 1543, 1560
Bsa29I ATCGAT 1 cut(s) 48
BsaBI GATNNNNATC 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 731, 951, 1224
Bse118I RCCGGY 1 cut(s) 124
Bse1I ACTGG 4 cut(s) 652, 765, 1071, 1091
Bse3DI GCAATG 1 cut(s) 530
Bse8I GATNNNNATC 1 cut(s) 84
BseCI ATCGAT 1 cut(s) 48
BseDI CCNNGG 3 cut(s) 731, 951, 1224
BseGI GGATG 5 cut(s) 442, 618, 1084, 1147, 1648
BseJI GATNNNNATC 1 cut(s) 84
BseMI GCAATG 1 cut(s) 530
BseMII CTCAG 4 cut(s) 201, 470, 762, 876
BseNI ACTGG 4 cut(s) 652, 765, 1071, 1091
BseRI GAGGAG 5 cut(s) 29, 90, 120, 807, 1360
BseXI GCAGC 4 cut(s) 313, 1535, 1596, 1811
Bsh1236I CGCG 1 cut(s) 290
BshFI GGCC 1 cut(s) 668
BshVI ATCGAT 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 1190
BsiSI CCGG 1 cut(s) 125
BslFI GGGAC 1 cut(s) 207
BsmAI GTCTC 4 cut(s) 424, 1225, 1726, 1767
BsmFI GGGAC 1 cut(s) 207
BsmI GAATGC 1 cut(s) 1090
BsnI GGCC 1 cut(s) 668
Bsp119I TTCGAA 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 1190
Bsp1720I GCTNAGC 1 cut(s) 1441
BspACI CCGC 7 cut(s) 134, 191, 245, 276, 983, 1658, 1891
BspANI GGCC 1 cut(s) 668
BspCNI CTCAG 4 cut(s) 200, 469, 761, 877
BspDI ATCGAT 1 cut(s) 48
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 2 cut(s) 129, 564
BspMAI CTGCAG 1 cut(s) 913
BspPI GGATC 3 cut(s) 218, 722, 1488
BspT104I TTCGAA 1 cut(s) 353
BspTI CTTAAG 2 cut(s) 232, 461
BsrDI GCAATG 1 cut(s) 530
BsrFI RCCGGY 1 cut(s) 124
BsrI ACTGG 4 cut(s) 652, 765, 1071, 1091
BssAI RCCGGY 1 cut(s) 124
BssECI CCNNGG 3 cut(s) 731, 951, 1224
BssT1I CCWWGG 2 cut(s) 951, 1224
Bst4CI ACNGT 5 cut(s) 316, 535, 1322, 1491, 1792
BstAFI CTTAAG 2 cut(s) 232, 461
BstBI TTCGAA 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 1891
BstDEI CTNAG 8 cut(s) 187, 456, 546, 748, 877, 885, 1441, 1921
BstF5I GGATG 5 cut(s) 442, 618, 1084, 1147, 1648
BstFNI CGCG 1 cut(s) 290
BstHHI GCGC 1 cut(s) 711
BstMAI GTCTC 4 cut(s) 424, 1225, 1726, 1767
BstMWI GCNNNNNNNGC 6 cut(s) 665, 989, 1202, 1415, 1523, 1734
BstNSI RCATGY 2 cut(s) 1028, 1280
BstSFI CTRYAG 1 cut(s) 909
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 4 cut(s) 313, 1535, 1596, 1811
BstV2I GAAGAC 1 cut(s) 953
BstX2I RGATCY 6 cut(s) 379, 727, 775, 1007, 1428, 1480
BstYI RGATCY 6 cut(s) 379, 727, 775, 1007, 1428, 1480
Bsu15I ATCGAT 1 cut(s) 48
BsuRI GGCC 1 cut(s) 668
BsuTUI ATCGAT 1 cut(s) 48
BtsCI GGATG 5 cut(s) 442, 618, 1084, 1147, 1648
BtsI GCAGTG 1 cut(s) 1855
BtsIMutI CAGTG 2 cut(s) 645, 1855
Cac8I GCNNGC 1 cut(s) 1891
CfoI GCGC 1 cut(s) 711
Cfr10I RCCGGY 1 cut(s) 124
ClaI ATCGAT 1 cut(s) 48
CseI GACGC 1 cut(s) 296
Csp6I GTAC 3 cut(s) 810, 1019, 1280
CviAII CATG 6 cut(s) 539, 1025, 1277, 1355, 1777, 1879
CviQI GTAC 3 cut(s) 810, 1019, 1280
DdeI CTNAG 8 cut(s) 187, 456, 546, 748, 877, 885, 1441, 1921
DraI TTTAAA 1 cut(s) 1102
EaeI YGGCCR 1 cut(s) 666
EciI GGCGGA 2 cut(s) 123, 234
Eco130I CCWWGG 2 cut(s) 951, 1224
Eco57I CTGAAG 2 cut(s) 567, 1515
EcoT14I CCWWGG 2 cut(s) 951, 1224
ErhI CCWWGG 2 cut(s) 951, 1224
FaeI CATG 6 cut(s) 542, 1028, 1280, 1358, 1780, 1882
FaqI GGGAC 1 cut(s) 207
FatI CATG 6 cut(s) 538, 1024, 1276, 1354, 1776, 1878
FauI CCCGC 2 cut(s) 184, 1898
Fnu4HI GCNGC 4 cut(s) 327, 1524, 1585, 1800
FokI GGATG 5 cut(s) 449, 625, 1091, 1154, 1635
Fsp4HI GCNGC 4 cut(s) 327, 1524, 1585, 1800
FspBI CTAG 6 cut(s) 492, 609, 698, 1122, 1206, 1520
GlaI GCGC 1 cut(s) 710
GluI GCNGC 4 cut(s) 327, 1524, 1585, 1800
GsuI CTGGAG 1 cut(s) 764
HaeIII GGCC 1 cut(s) 668
HapII CCGG 1 cut(s) 125
HgaI GACGC 1 cut(s) 296
HhaI GCGC 1 cut(s) 711
Hin1II CATG 6 cut(s) 542, 1028, 1280, 1358, 1780, 1882
Hin6I GCGC 1 cut(s) 709
HinP1I GCGC 1 cut(s) 709
HindIII AAGCTT 1 cut(s) 634
HpaII CCGG 1 cut(s) 125
HphI GGTGA 3 cut(s) 158, 1061, 1937
Hpy166II GTNNAC 1 cut(s) 1019
Hpy188I TCNGA 9 cut(s) 345, 679, 732, 751, 856, 886, 1375, 1477, 1633
Hpy8I GTNNAC 1 cut(s) 1019
Hpy99I CGWCG 3 cut(s) 32, 145, 363
HpyAV CCTTC 6 cut(s) 397, 460, 1259, 1388, 1430, 1927
HpyCH4III ACNGT 5 cut(s) 316, 535, 1322, 1491, 1792
HpyCH4IV ACGT 2 cut(s) 116, 415
HpyCH4V TGCA 4 cut(s) 175, 911, 1082, 1799
HpyF10VI GCNNNNNNNGC 6 cut(s) 665, 989, 1202, 1415, 1523, 1734
HpyF3I CTNAG 8 cut(s) 187, 456, 546, 748, 877, 885, 1441, 1921
HpySE526I ACGT 2 cut(s) 116, 415
Hsp92II CATG 6 cut(s) 542, 1028, 1280, 1358, 1780, 1882
HspAI GCGC 1 cut(s) 709
LmnI GCTCC 3 cut(s) 133, 406, 442
Lsp1109I GCAGC 4 cut(s) 313, 1535, 1596, 1811
LweI GCATC 3 cut(s) 1069, 1183, 1786
MaeI CTAG 6 cut(s) 492, 609, 698, 1122, 1206, 1520
MaeII ACGT 2 cut(s) 116, 415
MaeIII GTNAC 5 cut(s) 177, 319, 913, 1562, 1925
MboII GAAGA 8 cut(s) 29, 101, 157, 573, 858, 958, 1521, 1657
MfeI CAATTG 1 cut(s) 428
MflI RGATCY 6 cut(s) 379, 727, 775, 1007, 1428, 1480
MhlI GDGCHC 1 cut(s) 1190
MlsI TGGCCA 1 cut(s) 668
MluI ACGCGT 1 cut(s) 288
MluNI TGGCCA 1 cut(s) 668
MlyI GAGTC 4 cut(s) 497, 599, 1622, 1792
MmeI TCCRAC 3 cut(s) 137, 368, 1014
Mox20I TGGCCA 1 cut(s) 668
MroXI GAANNNNTTC 1 cut(s) 1293
MscI TGGCCA 1 cut(s) 668
MseI TTAA 9 cut(s) 24, 233, 293, 398, 462, 828, 1101, 1256, 1592
MslI CAYNNNNRTG 2 cut(s) 1044, 1178
Msp20I TGGCCA 1 cut(s) 668
MspA1I CMGCKG 1 cut(s) 191
MspCI CTTAAG 2 cut(s) 232, 461
MspI CCGG 1 cut(s) 125
MunI CAATTG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 1090
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 6 cut(s) 665, 989, 1202, 1415, 1523, 1734
NlaIII CATG 6 cut(s) 542, 1028, 1280, 1358, 1780, 1882
NlaIV GGNNCC 2 cut(s) 129, 564
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 2 cut(s) 1562, 1925
NspI RCATGY 2 cut(s) 1028, 1280
NspV TTCGAA 1 cut(s) 353
PciI ACATGT 2 cut(s) 1024, 1276
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PctI GAATGC 1 cut(s) 1090
PdmI GAANNNNTTC 1 cut(s) 1293
PfeI GAWTC 7 cut(s) 50, 355, 784, 881, 1111, 1346, 1634
PkrI GCNGC 4 cut(s) 328, 1525, 1586, 1801
PleI GAGTC 4 cut(s) 496, 599, 1622, 1791
PpsI GAGTC 4 cut(s) 496, 599, 1622, 1791
PscI ACATGT 2 cut(s) 1024, 1276
Psp1406I AACGTT 1 cut(s) 116
PspN4I GGNNCC 2 cut(s) 129, 564
PstI CTGCAG 1 cut(s) 913
PsuI RGATCY 6 cut(s) 379, 727, 775, 1007, 1428, 1480
RsaI GTAC 3 cut(s) 811, 1020, 1281
RsaNI GTAC 3 cut(s) 810, 1019, 1280
RseI CAYNNNNRTG 2 cut(s) 1044, 1178
SaqAI TTAA 9 cut(s) 24, 233, 293, 398, 462, 828, 1101, 1256, 1592
SatI GCNGC 4 cut(s) 327, 1524, 1585, 1800
SchI GAGTC 4 cut(s) 497, 599, 1622, 1792
SduI GDGCHC 1 cut(s) 1190
SfaNI GCATC 3 cut(s) 1069, 1183, 1786
SfcI CTRYAG 1 cut(s) 909
SfuI TTCGAA 1 cut(s) 353
SmiMI CAYNNNNRTG 2 cut(s) 1044, 1178
SmlI CTYRAG 5 cut(s) 232, 461, 819, 1558, 1575
SmoI CTYRAG 5 cut(s) 232, 461, 819, 1558, 1575
SsiI CCGC 7 cut(s) 134, 191, 245, 276, 983, 1658, 1891
SspMI CTAG 6 cut(s) 492, 609, 698, 1122, 1206, 1520
StyI CCWWGG 2 cut(s) 951, 1224
TaaI ACNGT 5 cut(s) 316, 535, 1322, 1491, 1792
TaiI ACGT 2 cut(s) 119, 418
TaqI TCGA 8 cut(s) 48, 57, 140, 353, 384, 422, 595, 1163
TatI WGTACW 1 cut(s) 1018
TfiI GAWTC 7 cut(s) 50, 355, 784, 881, 1111, 1346, 1634
Tru1I TTAA 9 cut(s) 24, 233, 293, 398, 462, 828, 1101, 1256, 1592
Tru9I TTAA 9 cut(s) 24, 233, 293, 398, 462, 828, 1101, 1256, 1592
TscAI CASTG 2 cut(s) 652, 1855
TseFI GTSAC 2 cut(s) 1562, 1925
TseI GCWGC 4 cut(s) 326, 1523, 1584, 1799
Tsp45I GTSAC 2 cut(s) 1562, 1925
TspGWI ACGGA 3 cut(s) 93, 217, 1297
TspRI CASTG 2 cut(s) 652, 1855
Vha464I CTTAAG 2 cut(s) 232, 461
XapI RAATTY 6 cut(s) 20, 32, 210, 940, 1399, 1643
XbaI TCTAGA 1 cut(s) 491
XceI RCATGY 2 cut(s) 1028, 1280
XmnI GAANNNNTTC 1 cut(s) 1293
XspI CTAG 6 cut(s) 492, 609, 698, 1122, 1206, 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.