FvH4_5g23380

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002469
Physical Location & Seq
Forward (+)
14732958 .. 14741722
8765 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23380.t8

Sequence Viewer

Length: 1431 bp
ATGTCGACCATGGACGACGACGACGACGGTTTAGATGATTGGGAACCGGATTTCGTCGAAGCGCTCATCAAAGAGCAAGAGCGCGTCATCTCCGATTCTCAGCCGCCGTTAAAACAAGAACCGCCGTCTCCGATGATCACCTACTCGCCTCCGCGCCACCTCTCCCAGCGCTCCAACGCCGTCTCCTCTTCCTCCGCGTTGCCGCCGCGTGTTCGGAGCTCCGAAGTTGGGAGATTAAAGATGGCTGCGTCTTCTTCGGCGTTGCCTCCTTGTGCTTCGGGCTCCTTTGGGAGTGAGAAGGATCGCGAAATTGGGCGATTGAAGAAGGAGCTTGGGGATGTCTTGAAGCGGCTTTCAGATTATGAACAAGAGTGCTCCAAACTGAAGAGAGAAAGAGACAGAAAAGATGAACAGCTTCAGTTTGTAATCTCAAGGAATGAAAAGAAAGATGGAGCTGACAGGATCTCTGATTGTGCAAATTTGTCCCATGGTGGCAGGGAATCTTCTGTACAATTACAAATGGCAAGAACTTCAAGTCATCACCCTAGCTCTCGGGGTCTTATTGGTGTGTCAACCTCTAAATCTGCAGGCATTCAAACTGATGAAATTACTGCCTCTGCCCAACCAATACAAAATGATGACCACCATGTATCTGATAACCTCTCCAAGAAATTGCTAGCTGTCTGGGGATCTCCAAATGAACAAGAGCTGGGGAAAAATGTAATTTCCAATTTGCTTAAGGCATGCCAAACAGATTTCCATTCTCTTTCTGGGTGCATTGGCATGAATATATCCTCCAAACTGGGAATGGACACTGTAGTAGCTTCACAGAATATGCACGATCATAAACCAGAAGTTGCAAAAGTATCTCATCTTTATTCGGTCTTGACCGAGGTCAATAATGGAATGATAAAGTTGGATGCTCTGTTTCAACCACTAGTCGACCTCTGCAGTCTTCAAAATGTTGTCACTGTCCAGGCATCTCTACATATACTACATGTGTTTCTTAAGCACCTTCTAAGTCTAGAAAGGCATGTAGAAGGAAGGGTCAATGTCATCATAGAGGGACTCGTAAGAAGGAATCAGACATCTTATGTGGACTCTCTTCCATTTGGAATGGATTTTTATGCTGAAACTGAAACGAAGAGTGAAGATTGGAACAATGGTGTTTCAACTTTGGTTTCTCGCATAGACTGGGATTTGCTTTTTGAGTTGATTGTACAAATTGCCACGAGGAATACAGAACACTGCGTAAGGCTACAAGCAGTGTCCATTATGAATGTCATTGTAATGAAAAGTAATGCCTTTATGGAGAGGGAAAAGTTTGGCCGGACACTGGTGTTTGAATTCATAGCAAAATTGCTTGCAAAGGAAGCTGGTTTTGAGGTGCGAAAACAAGCCGTGCAACTTCTGTATTTGCTACTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

477

Amino Acids

53.06

Weight (kDa)

5.96

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 5, 942
AccII CGCG 5 cut(s) 84, 154, 197, 208, 306
AciI CCGC 7 cut(s) 104, 122, 152, 195, 203, 206, 349
AclWI GGATC 3 cut(s) 309, 470, 697
AcoI YGGCCR 1 cut(s) 1327
AcsI RAATTY 2 cut(s) 478, 1346
AcuI CTGAAG 2 cut(s) 401, 404
AfaI GTAC 2 cut(s) 510, 1221
AfeI AGCGCT 2 cut(s) 63, 170
AfiI CCNNNNNNNGG 2 cut(s) 228, 1336
AflII CTTAAG 2 cut(s) 737, 1007
AflIII ACRYGT 1 cut(s) 997
AgsI TTSAA 8 cut(s) 322, 346, 534, 596, 932, 959, 1173, 1346
AhlI ACTAGT 1 cut(s) 937
AjnI CCWGG 1 cut(s) 975
AluBI AGCT 9 cut(s) 219, 331, 415, 455, 549, 680, 709, 824, 1376
AluI AGCT 9 cut(s) 219, 331, 415, 455, 549, 680, 709, 824, 1376
Alw21I GWGCWC 2 cut(s) 221, 377
Alw26I GTCTC 3 cut(s) 132, 187, 390
AlwI GGATC 3 cut(s) 309, 470, 697
Ama87I CYCGRG 1 cut(s) 552
Aor51HI AGCGCT 2 cut(s) 63, 170
AoxI GGCC 1 cut(s) 1327
ApeKI GCWGC 1 cut(s) 245
ApoI RAATTY 2 cut(s) 478, 1346
Asp700I GAANNNNTTC 1 cut(s) 414
AspLEI GCGC 4 cut(s) 64, 84, 156, 171
AsuHPI GGTGA 2 cut(s) 130, 533
AsuNHI GCTAGC 1 cut(s) 676
AvaI CYCGRG 1 cut(s) 552
BanII GRGCYC 2 cut(s) 221, 284
BauI CACGAG 1 cut(s) 1231
BbsI GAAGAC 2 cut(s) 243, 947
Bbv12I GWGCWC 2 cut(s) 221, 377
BbvI GCAGC 1 cut(s) 232
BccI CCATC 2 cut(s) 235, 443
BceAI ACGGC 4 cut(s) 91, 109, 164, 1385
BciT130I CCWGG 1 cut(s) 977
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 3 cut(s) 132, 187, 390
BcuI ACTAGT 1 cut(s) 937
BfaI CTAG 4 cut(s) 546, 677, 938, 1025
BfmI CTRYAG 3 cut(s) 585, 816, 949
BfoI RGCGCY 2 cut(s) 65, 172
BfrI CTTAAG 2 cut(s) 737, 1007
BisI GCNGC 5 cut(s) 104, 203, 206, 246, 350
BlsI GCNGC 5 cut(s) 105, 204, 207, 247, 351
Bme1390I CCNGG 1 cut(s) 977
BmeT110I CYCGRG 1 cut(s) 552
BmiI GGNNCC 2 cut(s) 45, 283
BmrFI CCNGG 1 cut(s) 977
BmrI ACTGGG 2 cut(s) 812, 1204
BmsI GCATC 2 cut(s) 910, 989
BmtI GCTAGC 1 cut(s) 680
BmuI ACTGGG 2 cut(s) 812, 1204
BoxI GACNNNNGTC 1 cut(s) 893
BpiI GAAGAC 2 cut(s) 243, 947
BpuEI CTTGAG 1 cut(s) 415
BsaJI CCNNGG 3 cut(s) 9, 487, 891
BsaWI WCCGGW 1 cut(s) 46
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 1336
Bse1I ACTGG 3 cut(s) 807, 1199, 1341
BseBI CCWGG 1 cut(s) 977
BseDI CCNNGG 3 cut(s) 9, 487, 891
BseGI GGATG 2 cut(s) 343, 925
BseLI CCNNNNNNNGG 2 cut(s) 228, 1336
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 3 cut(s) 807, 1199, 1341
BseRI GAGGAG 1 cut(s) 175
BseXI GCAGC 1 cut(s) 232
BseYI CCCAGC 2 cut(s) 165, 709
Bsh1236I CGCG 5 cut(s) 84, 154, 197, 208, 306
BshFI GGCC 1 cut(s) 1329
BsiHKAI GWGCWC 2 cut(s) 221, 377
BsiHKCI CYCGRG 1 cut(s) 552
BsiSI CCGG 2 cut(s) 47, 1330
BslFI GGGAC 2 cut(s) 469, 1080
BslI CCNNNNNNNGG 2 cut(s) 228, 1336
BsmAI GTCTC 3 cut(s) 132, 187, 390
BsmBI CGTCTC 2 cut(s) 132, 187
BsmFI GGGAC 2 cut(s) 469, 1080
BsmI GAATGC 1 cut(s) 591
BsnI GGCC 1 cut(s) 1329
BsoBI CYCGRG 1 cut(s) 552
Bsp1286I GDGCHC 3 cut(s) 221, 284, 377
Bsp1407I TGTACA 2 cut(s) 508, 1219
Bsp143I GATC 5 cut(s) 135, 301, 462, 689, 841
Bsp19I CCATGG 2 cut(s) 9, 487
Bsp68I TCGCGA 1 cut(s) 306
BspACI CCGC 7 cut(s) 104, 122, 152, 195, 203, 206, 349
BspANI GGCC 1 cut(s) 1329
BspCNI CTCAG 1 cut(s) 112
BspFNI CGCG 5 cut(s) 84, 154, 197, 208, 306
BspLI GGNNCC 2 cut(s) 45, 283
BspMAI CTGCAG 2 cut(s) 589, 953
BspOI GCTAGC 1 cut(s) 680
BspPI GGATC 3 cut(s) 309, 470, 697
BspTI CTTAAG 2 cut(s) 737, 1007
BsrGI TGTACA 2 cut(s) 508, 1219
BsrI ACTGG 3 cut(s) 807, 1199, 1341
BssECI CCNNGG 3 cut(s) 9, 487, 891
BssMI GATC 5 cut(s) 135, 301, 462, 689, 841
BssSI CACGAG 1 cut(s) 1231
BssT1I CCWWGG 2 cut(s) 9, 487
Bst2BI CACGAG 1 cut(s) 1231
Bst2UI CCWGG 1 cut(s) 977
Bst4CI ACNGT 3 cut(s) 29, 817, 973
Bst6I CTCTTC 4 cut(s) 193, 380, 1110, 1139
BstAFI CTTAAG 2 cut(s) 737, 1007
BstAUI TGTACA 2 cut(s) 508, 1219
BstC8I GCNNGC 4 cut(s) 589, 678, 745, 1365
BstDEI CTNAG 2 cut(s) 99, 1019
BstDSI CCRYGG 2 cut(s) 9, 487
BstF5I GGATG 2 cut(s) 343, 925
BstFNI CGCG 5 cut(s) 84, 154, 197, 208, 306
BstH2I RGCGCY 2 cut(s) 65, 172
BstHHI GCGC 4 cut(s) 64, 84, 156, 171
BstKTI GATC 5 cut(s) 138, 304, 465, 692, 844
BstMAI GTCTC 3 cut(s) 132, 187, 390
BstMBI GATC 5 cut(s) 135, 301, 462, 689, 841
BstMWI GCNNNNNNNGC 1 cut(s) 1373
BstNI CCWGG 1 cut(s) 977
BstNSI RCATGY 3 cut(s) 747, 1001, 1037
BstPAI GACNNNNGTC 1 cut(s) 893
BstSCI CCNGG 1 cut(s) 975
BstSFI CTRYAG 3 cut(s) 585, 816, 949
BstUI CGCG 5 cut(s) 84, 154, 197, 208, 306
BstV1I GCAGC 1 cut(s) 232
BstV2I GAAGAC 2 cut(s) 243, 947
BstX2I RGATCY 2 cut(s) 462, 689
BstYI RGATCY 2 cut(s) 462, 689
BsuRI GGCC 1 cut(s) 1329
BtgI CCRYGG 2 cut(s) 9, 487
BtsCI GGATG 2 cut(s) 343, 925
BtsI GCAGTG 2 cut(s) 1246, 1272
BtsIMutI CAGTG 5 cut(s) 813, 969, 1246, 1272, 1334
BtuMI TCGCGA 1 cut(s) 306
Cac8I GCNNGC 4 cut(s) 589, 678, 745, 1365
CfoI GCGC 4 cut(s) 64, 84, 156, 171
CseI GACGC 2 cut(s) 73, 237
Csp6I GTAC 2 cut(s) 509, 1220
CviAII CATG 7 cut(s) 10, 488, 647, 744, 784, 998, 1034
CviQI GTAC 2 cut(s) 509, 1220
DdeI CTNAG 2 cut(s) 99, 1019
DpnI GATC 5 cut(s) 137, 303, 464, 691, 843
DpnII GATC 5 cut(s) 135, 301, 462, 689, 841
EaeI YGGCCR 1 cut(s) 1327
Eam1104I CTCTTC 4 cut(s) 193, 380, 1110, 1139
EarI CTCTTC 4 cut(s) 193, 380, 1110, 1139
Ecl136II GAGCTC 1 cut(s) 219
Eco130I CCWWGG 2 cut(s) 9, 487
Eco24I GRGCYC 2 cut(s) 221, 284
Eco47III AGCGCT 2 cut(s) 63, 170
Eco53kI GAGCTC 1 cut(s) 219
Eco57I CTGAAG 2 cut(s) 401, 404
Eco88I CYCGRG 1 cut(s) 552
EcoICRI GAGCTC 1 cut(s) 219
EcoRI GAATTC 1 cut(s) 1346
EcoRII CCWGG 1 cut(s) 975
EcoT14I CCWWGG 2 cut(s) 9, 487
EcoT38I GRGCYC 2 cut(s) 221, 284
ErhI CCWWGG 2 cut(s) 9, 487
Esp3I CGTCTC 2 cut(s) 132, 187
FaeI CATG 7 cut(s) 13, 491, 650, 747, 787, 1001, 1037
FaqI GGGAC 2 cut(s) 469, 1080
FatI CATG 7 cut(s) 9, 487, 646, 743, 783, 997, 1033
FbaI TGATCA 1 cut(s) 135
FblI GTMKAC 2 cut(s) 5, 942
Fnu4HI GCNGC 5 cut(s) 104, 203, 206, 246, 350
FokI GGATG 2 cut(s) 350, 932
FriOI GRGCYC 2 cut(s) 221, 284
Fsp4HI GCNGC 5 cut(s) 104, 203, 206, 246, 350
FspBI CTAG 4 cut(s) 546, 677, 938, 1025
GlaI GCGC 4 cut(s) 63, 83, 155, 170
GluI GCNGC 5 cut(s) 104, 203, 206, 246, 350
GsaI CCCAGC 2 cut(s) 169, 713
HaeII RGCGCY 2 cut(s) 65, 172
HaeIII GGCC 1 cut(s) 1329
HapII CCGG 2 cut(s) 47, 1330
HgaI GACGC 2 cut(s) 73, 237
HhaI GCGC 4 cut(s) 64, 84, 156, 171
Hin1II CATG 7 cut(s) 13, 491, 650, 747, 787, 1001, 1037
Hin6I GCGC 4 cut(s) 62, 82, 154, 169
HinP1I GCGC 4 cut(s) 62, 82, 154, 169
HincII GTYRAC 3 cut(s) 6, 573, 943
HindII GTYRAC 3 cut(s) 6, 573, 943
HinfI GANTC 5 cut(s) 95, 500, 1068, 1081, 1100
HpaII CCGG 2 cut(s) 47, 1330
HphI GGTGA 2 cut(s) 130, 533
Hpy166II GTNNAC 4 cut(s) 6, 573, 943, 1099
Hpy188I TCNGA 8 cut(s) 94, 132, 216, 223, 358, 469, 655, 1086
Hpy188III TCNNGA 4 cut(s) 305, 343, 886, 1025
Hpy8I GTNNAC 4 cut(s) 6, 573, 943, 1099
Hpy99I CGWCG 5 cut(s) 20, 23, 26, 29, 59
HpyAV CCTTC 6 cut(s) 292, 319, 1025, 1034, 1038, 1071
HpyCH4III ACNGT 3 cut(s) 29, 817, 973
HpyCH4V TGCA 8 cut(s) 476, 587, 777, 838, 860, 951, 1367, 1405
HpyF10VI GCNNNNNNNGC 1 cut(s) 1373
HpyF3I CTNAG 2 cut(s) 99, 1019
Hsp92II CATG 7 cut(s) 13, 491, 650, 747, 787, 1001, 1037
HspAI GCGC 4 cut(s) 62, 82, 154, 169
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 5 cut(s) 135, 301, 462, 689, 841
LmnI GCTCC 7 cut(s) 176, 216, 224, 287, 328, 380, 452
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 2 cut(s) 910, 989
MaeI CTAG 4 cut(s) 546, 677, 938, 1025
MaeIII GTNAC 1 cut(s) 967
MalI GATC 5 cut(s) 137, 303, 464, 691, 843
MboI GATC 5 cut(s) 135, 301, 462, 689, 841
MflI RGATCY 2 cut(s) 462, 689
MhlI GDGCHC 3 cut(s) 221, 284, 377
MlyI GAGTC 2 cut(s) 1062, 1094
MmeI TCCRAC 2 cut(s) 198, 897
MroXI GAANNNNTTC 1 cut(s) 414
MseI TTAA 4 cut(s) 110, 236, 738, 1008
MslI CAYNNNNRTG 2 cut(s) 782, 1289
MspCI CTTAAG 2 cut(s) 737, 1007
MspI CCGG 2 cut(s) 47, 1330
MspR9I CCNGG 1 cut(s) 977
Mva1269I GAATGC 1 cut(s) 591
MvaI CCWGG 1 cut(s) 977
MvnI CGCG 5 cut(s) 84, 154, 197, 208, 306
MwoI GCNNNNNNNGC 1 cut(s) 1373
NcoI CCATGG 2 cut(s) 9, 487
NdeII GATC 5 cut(s) 135, 301, 462, 689, 841
NheI GCTAGC 1 cut(s) 676
NlaIII CATG 7 cut(s) 13, 491, 650, 747, 787, 1001, 1037
NlaIV GGNNCC 2 cut(s) 45, 283
NmuCI GTSAC 1 cut(s) 967
NruI TCGCGA 1 cut(s) 306
NspI RCATGY 3 cut(s) 747, 1001, 1037
PaeI GCATGC 1 cut(s) 747
PciI ACATGT 1 cut(s) 997
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PctI GAATGC 1 cut(s) 591
PdmI GAANNNNTTC 1 cut(s) 414
PfeI GAWTC 3 cut(s) 95, 500, 1081
PkrI GCNGC 5 cut(s) 105, 204, 207, 247, 351
PleI GAGTC 2 cut(s) 1062, 1094
PpsI GAGTC 2 cut(s) 1062, 1094
PscI ACATGT 1 cut(s) 997
PshAI GACNNNNGTC 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 221
Psp6I CCWGG 1 cut(s) 975
PspFI CCCAGC 2 cut(s) 165, 709
PspGI CCWGG 1 cut(s) 975
PspN4I GGNNCC 2 cut(s) 45, 283
PstI CTGCAG 2 cut(s) 589, 953
PsuI RGATCY 2 cut(s) 462, 689
RruI TCGCGA 1 cut(s) 306
RsaI GTAC 2 cut(s) 510, 1221
RsaNI GTAC 2 cut(s) 509, 1220
RseI CAYNNNNRTG 2 cut(s) 782, 1289
SacI GAGCTC 1 cut(s) 221
SalI GTCGAC 2 cut(s) 4, 941
SaqAI TTAA 4 cut(s) 110, 236, 738, 1008
SatI GCNGC 5 cut(s) 104, 203, 206, 246, 350
Sau3AI GATC 5 cut(s) 135, 301, 462, 689, 841
SchI GAGTC 2 cut(s) 1062, 1094
ScrFI CCNGG 1 cut(s) 977
SduI GDGCHC 3 cut(s) 221, 284, 377
SfaNI GCATC 2 cut(s) 910, 989
SfcI CTRYAG 3 cut(s) 585, 816, 949
SmiMI CAYNNNNRTG 2 cut(s) 782, 1289
SmlI CTYRAG 3 cut(s) 430, 737, 1007
SmoI CTYRAG 3 cut(s) 430, 737, 1007
SpeI ACTAGT 1 cut(s) 937
SphI GCATGC 1 cut(s) 747
SsiI CCGC 7 cut(s) 104, 122, 152, 195, 203, 206, 349
SspMI CTAG 4 cut(s) 546, 677, 938, 1025
SstI GAGCTC 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 975
StyI CCWWGG 2 cut(s) 9, 487
TaaI ACNGT 3 cut(s) 29, 817, 973
TaqI TCGA 3 cut(s) 5, 57, 942
TaqII GACCGA 2 cut(s) 871, 905
TatI WGTACW 2 cut(s) 508, 1219
TauI GCSGC 4 cut(s) 106, 205, 208, 352
TfiI GAWTC 3 cut(s) 95, 500, 1081
Tru1I TTAA 4 cut(s) 110, 236, 738, 1008
Tru9I TTAA 4 cut(s) 110, 236, 738, 1008
TscAI CASTG 5 cut(s) 820, 976, 1253, 1272, 1341
TseFI GTSAC 1 cut(s) 967
TseI GCWGC 1 cut(s) 245
Tsp45I GTSAC 1 cut(s) 967
TspDTI ATGAA 9 cut(s) 378, 423, 453, 618, 714, 800, 1292, 1307, 1339
TspRI CASTG 5 cut(s) 820, 976, 1253, 1272, 1341
Vha464I CTTAAG 2 cut(s) 737, 1007
XapI RAATTY 2 cut(s) 478, 1346
XbaI TCTAGA 1 cut(s) 1024
XceI RCATGY 3 cut(s) 747, 1001, 1037
XcmI CCANNNNNNNNNTGG 2 cut(s) 767, 805
XmiI GTMKAC 2 cut(s) 5, 942
XmnI GAANNNNTTC 1 cut(s) 414
XspI CTAG 4 cut(s) 546, 677, 938, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.