Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0012217

Physical Location & Seq

Genomic Coordinates Forward (+)

18615175 .. 18618597

Gene Structure 3423 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G45900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2094 bp

ATGGCTGAGAAAGAAACTCCAGCAATCATACTTCAATTCGCTCCATTGAACAGTTCCGTCGACGAAGGTTTCTGGCATAGCTTCTCCTCTTTGAAACTCGACAAACTCGGAATCGATGATTCTCCGATTTCAATCACCGGTTTCTATGGACCATGTGGACATCCACAAGTCTCAAATCATTTGACTCTTTTATCAGAGTCATTGCCTTTAGATGAACAATCATTGATTGCTAGTACTAGTCATGGAAATAGGAATAAGTGTCCTGTTCCTGGGATTTTGTATAACACCAATACCGTTGAAAGCTTCAATAAACTTGACAAACAAAGTCTGCTAAAAGCAGAAGCAAATAAGATTTGGGAGGATATTCAATCAGGAAAAGCTCTTGAAGACCCTTCTGTGTTACCAAGATTCCTCGTTATCTCCTTTGCAGACCTTAAGAAATGGAGTTTTCGGTATTGGTTTGCGTTCCCTGCGTTTGTACTTGATCCTCCTGTAAGTTTGATTGAATTAAAGCCAGCTTCAGAGTATTTTAGTTCTGAAGAGGCTGAATCAGTATCCGCTGCTTGTAATGATTGGCGCGACTCAGATTTAACTACTGATGTTCCATTCTTTTTGGTTTCGGTTTCTTCTGATTCAAAAGCTAGTATCAGACATCTTAAAGACTTGGAAGCGTGCCAAGGTGATCATCAAAAGTTGCTTTTCGGTTTCTATGACCCGTGTCACCTTCCTAGTAATCCTGGCTGGCCACTTCGAAACTACTTGGCACTGATTCGCTCAAGATGGAACCTCGAGACAGTTTGGTTCTTCTGCTACAGAGAGAGTCGTGGTTTCGCTGACTTGAACCTTTCTCTTGTTGGTCAAGCCTCAATTACACTTTCATCAGGTGAATCAGCTGAGACTGTACCTAACTCAGTGGGATGGGAGCTTAACAAAGGGAAACGTGTACCCCGATCCATTAGCCTTGCTAATTCCATGGATCCAACAAGGTTGGCTGTTTCTGCTGTCGATTTAAACTTAAAGTTAATGAGATGGCGAGCATTACCGTCTCTTAACTTAAATGTATTGTCCTCTGTCAAATGCCTTCTTCTTGGAGCTGGTACATTGGGTTGCCAAGTTGCTCGTACCCTTATGGGTTGGGGAATCCGCAATATCACCTTTGTTGACTATGGAAAAGTTGCCATGTCTAATCCAGTCAGGCAATCTCTCTATAATTTTGAAGATTGTCTAGGTCGTGGTGAGTTTAAAGCTGTTGCTGCTGTTAAAAGCCTTAAACAGATATTTCCAGCAATGGAAACTAGTGGAGTTGTTATGGCTATACCGATGCCCGGACATCCGATTTCTAGCCAAGAAGAAGATTCTGTTCTCGGTGATTGTAAACGCCTTAGCGAGTTGATTGAATCTCATGATGCGGTGTTCTTGTTAACCGATACAAGAGAAAGCAGGTGGTTACCGTCTCTTCTTTGTGCTAATGCCAATAAGATCGCTATAAACGCGGCTTTAGGTTTTGACAGCTACATGGTAATGCGCCATGGTGCTGGCCCCACCTCTCTGTCTGATGATATGCAGAATCTTGACATAAACAAGACAAATACACAGAGACTTGGTTGTTACTTCTGCAACGACGTGGTTGCACCTCAGGATTCAATGACTGATCGAACTCTAGACCAACAATGCACTGTTACACGCCCGGGTCTAGCTCCTATTGCAGGAGCTCTCGCCGTTGAACTCTTAGTCGGAGTCCTACAACACCCACTTGGTATCAATGCAAAAGGCGACAATTCGAGCTTGAGTAATACAGGAAACAATGATGATTCGCCTCTCGGGATATTACCTCATCAGATACGAGGCTCAGTTTCTCAGTTCTCACAGATCACGCTGCTTGGTCAAGCTTCCAACAGCTGTACCGCTTGCTCTGAAACCGTGATATCGGAGTATAGGGAGAGAGGAAACAGTTTCATACTTGAAGCTATTAACCATCCTACATACCTGGAGGATCTTACTGGTTTAACCGAGCTTAAGAAAGCTGCTAATTCATTCAACCTCGACTGGGAAGACGATGATACTGATGATGACGATGTAGCTGTAGATCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000407 GO:0000422 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006497 GO:0006501 GO:0006807 GO:0006914 GO:0006950 GO:0006952 GO:0006995 GO:0006996 GO:0007005 GO:0007033 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008641 GO:0009056 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0009991 GO:0010150 GO:0016043 GO:0016236 GO:0016237 GO:0016740 GO:0016874 GO:0016877 GO:0018410 GO:0019538 GO:0019778 GO:0019779 GO:0022411 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0034645 GO:0034727 GO:0036211 GO:0042157 GO:0042158 GO:0042594 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043687 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044804 GO:0044805 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061726 GO:0061919 GO:0070925 GO:0071496 GO:0071704 GO:0071840 GO:0090693 GO:0098542 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1903008 GO:1905037

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 ko04216 map04136 map04138 map04140 map04216

Pfam Domains

Protein Families

ATG7_N ThiF

Protein Analysis

697

Amino Acids

76.52

Weight (kDa)

4.95

Isoelectric Point (pI)

45.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG7_N	PF16420	11 - 325	4.9e-103	Ubiquitin-like modifier-activating enzyme ATG7 N-terminus
ThiF	PF00899	342 - 584	1.3e-37	ThiF family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45900
fragaria_vesca	FvH4_3g07910
malus_domestica	MD05G1302300.v1.1 MD10G1281000.v1.1
prunus_persica	Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1
pyrus_communis	pycom05g27990 pycom10g23460
rosa_chinensis	RchiOBHm_Chr5g0010641
rosa_laevigata	RLG00000031768
rosa_multiflora	Rmu_sc0006103.1_g000002 Rmu_sc0014635.1_g000003
rosa_roxburghii	Rroxscaffold_1G00065730
rosa_rugosa	Rorug04G0451400.1
rosa_samantha	Rh5AG083000 Rh5CG091000
rosa_wichuraiana	Rw0G000680 Rw0G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1431
Acc36I	ACCTGC	1 cut(s)	1431
AccI	GTMKAC	1 cut(s)	60
AccII	CGCG	2 cut(s)	579, 1493
AciI	CCGC	5 cut(s)	558, 1144, 1409, 1493, 1905
AclWI	GGATC	5 cut(s)	479, 945, 971, 984, 2001
AcoI	YGGCCR	1 cut(s)	743
AcuI	CTGAAG	2 cut(s)	504, 558
AfaI	GTAC	7 cut(s)	235, 480, 903, 945, 1099, 1123, 1903
AfiI	CCNNNNNNNGG	4 cut(s)	269, 1325, 1706, 2047
AflII	CTTAAG	2 cut(s)	434, 2015
AflIII	ACRYGT	1 cut(s)	940
AgeI	ACCGGT	1 cut(s)	137
AhlI	ACTAGT	2 cut(s)	236, 1295
AjiI	CACGTC	1 cut(s)	1624
AjnI	CCWGG	3 cut(s)	268, 736, 1986
Alw21I	GWGCWC	1 cut(s)	1714
Alw26I	GTCTC	6 cut(s)	175, 785, 890, 1050, 1458, 1591
AlwI	GGATC	5 cut(s)	479, 945, 971, 984, 2001
Ama87I	CYCGRG	3 cut(s)	788, 1687, 1820
AoxI	GGCC	2 cut(s)	743, 1537
ApeKI	GCWGC	4 cut(s)	560, 1253, 1876, 2024
AsiGI	ACCGGT	1 cut(s)	137
AspLEI	GCGC	2 cut(s)	579, 1527
AspS9I	GGNCC	2 cut(s)	149, 1538
AsuC2I	CCSGG	3 cut(s)	1326, 1688, 1689
AsuHPI	GGTGA	7 cut(s)	127, 692, 713, 896, 1144, 1247, 1379
AsuII	TTCGAA	1 cut(s)	751
AvaI	CYCGRG	3 cut(s)	788, 1687, 1820
AvaII	GGWCC	1 cut(s)	149
AxyI	CCTNAGG	1 cut(s)	1635
BaeI	ACNNNNGTAYC	2 cut(s)	1885, 1918
BalI	TGGCCA	1 cut(s)	745
BamHI	GGATCC	1 cut(s)	976
BanII	GRGCYC	1 cut(s)	1714
BbsI	GAAGAC	2 cut(s)	393, 2058
Bbv12I	GWGCWC	1 cut(s)	1714
BbvI	GCAGC	4 cut(s)	547, 1240, 1863, 2011
BccI	CCATC	4 cut(s)	774, 912, 1023, 1983
BceAI	ACGGC	1 cut(s)	1703
BcgI	CGANNNNNNTGC	2 cut(s)	1610, 1644
BciT130I	CCWGG	3 cut(s)	270, 738, 1988
BciVI	GTATCC	1 cut(s)	565
BclI	TGATCA	1 cut(s)	682
BcnI	CCSGG	3 cut(s)	1326, 1688, 1689
BcoDI	GTCTC	6 cut(s)	175, 785, 890, 1050, 1458, 1591
BcuI	ACTAGT	2 cut(s)	236, 1295
BfaI	CTAG	9 cut(s)	231, 237, 642, 729, 1226, 1296, 1341, 1661, 1694
BfmI	CTRYAG	2 cut(s)	811, 2082
BfrI	CTTAAG	2 cut(s)	434, 2015
BfuAI	ACCTGC	1 cut(s)	1431
BfuI	GTATCC	1 cut(s)	565
BglII	AGATCT	1 cut(s)	2086
BisI	GCNGC	5 cut(s)	561, 1254, 1494, 1877, 2025
BlsI	GCNGC	5 cut(s)	562, 1255, 1495, 1878, 2026
BmcAI	AGTACT	1 cut(s)	235
Bme1390I	CCNGG	6 cut(s)	270, 738, 1326, 1688, 1689, 1988
Bme18I	GGWCC	1 cut(s)	149
BmeT110I	CYCGRG	3 cut(s)	788, 1687, 1820
BmgBI	CACGTC	1 cut(s)	1624
BmgT120I	GGNCC	2 cut(s)	149, 1538
BmiI	GGNNCC	3 cut(s)	785, 978, 1540
BmrFI	CCNGG	6 cut(s)	270, 738, 1326, 1688, 1689, 1988
BmrI	ACTGGG	1 cut(s)	2056
BmsI	GCATC	2 cut(s)	1311, 1396
BmuI	ACTGGG	1 cut(s)	2056
BoxI	GACNNNNGTC	1 cut(s)	717
BpiI	GAAGAC	2 cut(s)	393, 2058
BpmI	CTGGAG	1 cut(s)	2009
Bpu10I	CCTNAGC	1 cut(s)	1382
Bpu14I	TTCGAA	1 cut(s)	751
BpuEI	CTTGAG	2 cut(s)	760, 1807
BpuMI	CCSGG	3 cut(s)	1326, 1688, 1689
Bsa29I	ATCGAT	1 cut(s)	114
BsaBI	GATNNNNATC	1 cut(s)	131
BsaJI	CCNNGG	5 cut(s)	269, 676, 972, 1528, 1687
BsaWI	WCCGGW	1 cut(s)	137
Bsc4I	CCNNNNNNNGG	4 cut(s)	269, 1325, 1706, 2047
Bse118I	RCCGGY	1 cut(s)	137
Bse1I	ACTGG	3 cut(s)	1190, 2005, 2051
Bse21I	CCTNAGG	1 cut(s)	1635
Bse3DI	GCAATG	2 cut(s)	200, 1293
Bse8I	GATNNNNATC	1 cut(s)	131
BseBI	CCWGG	3 cut(s)	270, 738, 1988
BseCI	ATCGAT	1 cut(s)	114
BseDI	CCNNGG	5 cut(s)	269, 676, 972, 1528, 1687
BseGI	GGATG	4 cut(s)	160, 923, 1330, 1975
BseJI	GATNNNNATC	1 cut(s)	131
BseLI	CCNNNNNNNGG	4 cut(s)	269, 1325, 1706, 2047
BseMI	GCAATG	2 cut(s)	200, 1293
BseMII	CTCAG	6 cut(s)	597, 885, 924, 1649, 1863, 1871
BseNI	ACTGG	3 cut(s)	1190, 2005, 2051
BseRI	GAGGAG	1 cut(s)	76
BseXI	GCAGC	4 cut(s)	547, 1240, 1863, 2011
Bsh1236I	CGCG	2 cut(s)	579, 1493
BshFI	GGCC	2 cut(s)	745, 1539
BshTI	ACCGGT	1 cut(s)	137
BshVI	ATCGAT	1 cut(s)	114
BsiHKAI	GWGCWC	1 cut(s)	1714
BsiHKCI	CYCGRG	3 cut(s)	788, 1687, 1820
BsiSI	CCGG	3 cut(s)	138, 1326, 1688
BslI	CCNNNNNNNGG	4 cut(s)	269, 1325, 1706, 2047
BsmAI	GTCTC	6 cut(s)	175, 785, 890, 1050, 1458, 1591
BsmBI	CGTCTC	2 cut(s)	1050, 1458
BsnI	GGCC	2 cut(s)	745, 1539
BsoBI	CYCGRG	3 cut(s)	788, 1687, 1820
Bsp119I	TTCGAA	1 cut(s)	751
Bsp1286I	GDGCHC	1 cut(s)	1714
Bsp143I	GATC	9 cut(s)	484, 682, 950, 976, 1479, 1651, 1869, 1993, 2086
Bsp19I	CCATGG	2 cut(s)	972, 1528
BspACI	CCGC	5 cut(s)	558, 1144, 1409, 1493, 1905
BspANI	GGCC	2 cut(s)	745, 1539
BspCNI	CTCAG	6 cut(s)	596, 886, 923, 1648, 1862, 1870
BspDI	ATCGAT	1 cut(s)	114
BspFNI	CGCG	2 cut(s)	579, 1493
BspHI	TCATGA	1 cut(s)	1402
BspLI	GGNNCC	3 cut(s)	785, 978, 1540
BspMI	ACCTGC	1 cut(s)	1431
BspPI	GGATC	5 cut(s)	479, 945, 971, 984, 2001
BspT104I	TTCGAA	1 cut(s)	751
BspTI	CTTAAG	2 cut(s)	434, 2015
BsrDI	GCAATG	2 cut(s)	200, 1293
BsrFI	RCCGGY	1 cut(s)	137
BsrI	ACTGG	3 cut(s)	1190, 2005, 2051
BssAI	RCCGGY	1 cut(s)	137
BssECI	CCNNGG	5 cut(s)	269, 676, 972, 1528, 1687
BssMI	GATC	9 cut(s)	484, 682, 950, 976, 1479, 1651, 1869, 1993, 2086
BssT1I	CCWWGG	3 cut(s)	676, 972, 1528
Bst2UI	CCWGG	3 cut(s)	270, 738, 1988
Bst4CI	ACNGT	9 cut(s)	53, 295, 796, 901, 1044, 1452, 1678, 1921, 1952
Bst6I	CTCTTC	2 cut(s)	534, 1461
BstAFI	CTTAAG	2 cut(s)	434, 2015
BstBI	TTCGAA	1 cut(s)	751
BstC8I	GCNNGC	6 cut(s)	516, 673, 743, 1035, 1537, 1909
BstDEI	CTNAG	9 cut(s)	6, 583, 894, 910, 1382, 1635, 1729, 1849, 1857
BstDSI	CCRYGG	2 cut(s)	972, 1528
BstEII	GGTNACC	1 cut(s)	1446
BstENI	CCTNNNNNAGG	1 cut(s)	1704
BstF5I	GGATG	4 cut(s)	160, 923, 1330, 1975
BstFNI	CGCG	2 cut(s)	579, 1493
BstHHI	GCGC	2 cut(s)	579, 1527
BstKTI	GATC	9 cut(s)	487, 685, 953, 979, 1482, 1654, 1872, 1996, 2089
BstMAI	GTCTC	6 cut(s)	175, 785, 890, 1050, 1458, 1591
BstMBI	GATC	9 cut(s)	484, 682, 950, 976, 1479, 1651, 1869, 1993, 2086
BstMWI	GCNNNNNNNGC	5 cut(s)	470, 998, 1253, 1490, 1703
BstNI	CCWGG	3 cut(s)	270, 738, 1988
BstPAI	GACNNNNGTC	1 cut(s)	717
BstPI	GGTNACC	1 cut(s)	1446
BstSCI	CCNGG	6 cut(s)	268, 736, 1324, 1686, 1687, 1986
BstSFI	CTRYAG	2 cut(s)	811, 2082
BstUI	CGCG	2 cut(s)	579, 1493
BstV1I	GCAGC	4 cut(s)	547, 1240, 1863, 2011
BstV2I	GAAGAC	2 cut(s)	393, 2058
BstX2I	RGATCY	3 cut(s)	976, 1993, 2086
BstXI	CCANNNNNNTGG	1 cut(s)	1535
BstYI	RGATCY	3 cut(s)	976, 1993, 2086
Bsu15I	ATCGAT	1 cut(s)	114
Bsu36I	CCTNAGG	1 cut(s)	1635
BsuI	GTATCC	1 cut(s)	565
BsuRI	GGCC	2 cut(s)	745, 1539
BsuTUI	ATCGAT	1 cut(s)	114
BtgI	CCRYGG	2 cut(s)	972, 1528
BtrI	CACGTC	1 cut(s)	1624
BtsCI	GGATG	4 cut(s)	160, 923, 1330, 1975
BtsIMutI	CAGTG	3 cut(s)	764, 918, 1674
BveI	ACCTGC	1 cut(s)	1431
Cac8I	GCNNGC	6 cut(s)	516, 673, 743, 1035, 1537, 1909
CciI	TCATGA	1 cut(s)	1402
CfoI	GCGC	2 cut(s)	579, 1527
Cfr10I	RCCGGY	1 cut(s)	137
Cfr13I	GGNCC	2 cut(s)	149, 1538
Cfr9I	CCCGGG	1 cut(s)	1687
ClaI	ATCGAT	1 cut(s)	114
Csp6I	GTAC	7 cut(s)	234, 479, 902, 944, 1098, 1122, 1902
CspAI	ACCGGT	1 cut(s)	137
CviAII	CATG	7 cut(s)	153, 242, 973, 1180, 1403, 1516, 1529
CviQI	GTAC	7 cut(s)	234, 479, 902, 944, 1098, 1122, 1902
DdeI	CTNAG	9 cut(s)	6, 583, 894, 910, 1382, 1635, 1729, 1849, 1857
DpnI	GATC	9 cut(s)	486, 684, 952, 978, 1481, 1653, 1871, 1995, 2088
DpnII	GATC	9 cut(s)	484, 682, 950, 976, 1479, 1651, 1869, 1993, 2086
DraI	TTTAAA	2 cut(s)	1011, 1243
EaeI	YGGCCR	1 cut(s)	743
Eam1104I	CTCTTC	2 cut(s)	534, 1461
EarI	CTCTTC	2 cut(s)	534, 1461
Ecl136II	GAGCTC	1 cut(s)	1712
Eco130I	CCWWGG	3 cut(s)	676, 972, 1528
Eco24I	GRGCYC	1 cut(s)	1714
Eco32I	GATATC	1 cut(s)	1926
Eco47I	GGWCC	1 cut(s)	149
Eco53kI	GAGCTC	1 cut(s)	1712
Eco57I	CTGAAG	2 cut(s)	504, 558
Eco81I	CCTNAGG	1 cut(s)	1635
Eco88I	CYCGRG	3 cut(s)	788, 1687, 1820
Eco91I	GGTNACC	1 cut(s)	1446
EcoICRI	GAGCTC	1 cut(s)	1712
EcoNI	CCTNNNNNAGG	1 cut(s)	1704
EcoO65I	GGTNACC	1 cut(s)	1446
EcoRII	CCWGG	3 cut(s)	268, 736, 1986
EcoRV	GATATC	1 cut(s)	1926
EcoT14I	CCWWGG	3 cut(s)	676, 972, 1528
EcoT38I	GRGCYC	1 cut(s)	1714
ErhI	CCWWGG	3 cut(s)	676, 972, 1528
Esp3I	CGTCTC	2 cut(s)	1050, 1458
FaeI	CATG	7 cut(s)	156, 245, 976, 1183, 1406, 1519, 1532
FatI	CATG	7 cut(s)	152, 241, 972, 1179, 1402, 1515, 1528
FbaI	TGATCA	1 cut(s)	682
FblI	GTMKAC	1 cut(s)	60
Fnu4HI	GCNGC	5 cut(s)	561, 1254, 1494, 1877, 2025
FokI	GGATG	4 cut(s)	147, 930, 1317, 1962
FriOI	GRGCYC	1 cut(s)	1714
Fsp4HI	GCNGC	5 cut(s)	561, 1254, 1494, 1877, 2025
FspBI	CTAG	9 cut(s)	231, 237, 642, 729, 1226, 1296, 1341, 1661, 1694
GlaI	GCGC	2 cut(s)	578, 1526
GluI	GCNGC	5 cut(s)	561, 1254, 1494, 1877, 2025
GsuI	CTGGAG	1 cut(s)	2009
HaeIII	GGCC	2 cut(s)	745, 1539
HapII	CCGG	3 cut(s)	138, 1326, 1688
HhaI	GCGC	2 cut(s)	579, 1527
Hin1II	CATG	7 cut(s)	156, 245, 976, 1183, 1406, 1519, 1532
Hin6I	GCGC	2 cut(s)	577, 1525
HinP1I	GCGC	2 cut(s)	577, 1525
HincII	GTYRAC	3 cut(s)	61, 1162, 1422
HindII	GTYRAC	3 cut(s)	61, 1162, 1422
HindIII	AAGCTT	2 cut(s)	301, 1887
HpaI	GTTAAC	1 cut(s)	1422
HpaII	CCGG	3 cut(s)	138, 1326, 1688
HphI	GGTGA	7 cut(s)	127, 692, 713, 896, 1144, 1247, 1379
Hpy166II	GTNNAC	6 cut(s)	61, 158, 944, 1162, 1376, 1422
Hpy188III	TCNNGA	9 cut(s)	372, 383, 777, 790, 1403, 1571, 1637, 1661, 1822
Hpy8I	GTNNAC	6 cut(s)	61, 158, 944, 1162, 1376, 1422
Hpy99I	CGWCG	3 cut(s)	62, 65, 1625
HpyAV	CCTTC	4 cut(s)	59, 402, 734, 1091
HpyCH4III	ACNGT	9 cut(s)	53, 295, 796, 901, 1044, 1452, 1678, 1921, 1952
HpyCH4IV	ACGT	2 cut(s)	940, 1623
HpyCH4V	TGCA	7 cut(s)	428, 1564, 1617, 1631, 1674, 1706, 1766
HpyF10VI	GCNNNNNNNGC	5 cut(s)	470, 998, 1253, 1490, 1703
HpyF3I	CTNAG	9 cut(s)	6, 583, 894, 910, 1382, 1635, 1729, 1849, 1857
HpySE526I	ACGT	2 cut(s)	940, 1623
Hsp92II	CATG	7 cut(s)	156, 245, 976, 1183, 1406, 1519, 1532
HspAI	GCGC	2 cut(s)	577, 1525
Ksp22I	TGATCA	1 cut(s)	682
KspAI	GTTAAC	1 cut(s)	1422
Kzo9I	GATC	9 cut(s)	484, 682, 950, 976, 1479, 1651, 1869, 1993, 2086
LmnI	GCTCC	5 cut(s)	46, 922, 1091, 1702, 1709
Lsp1109I	GCAGC	4 cut(s)	547, 1240, 1863, 2011
LweI	GCATC	2 cut(s)	1311, 1396
MaeI	CTAG	9 cut(s)	231, 237, 642, 729, 1226, 1296, 1341, 1661, 1694
MaeII	ACGT	2 cut(s)	940, 1623
MaeIII	GTNAC	5 cut(s)	399, 719, 1446, 1607, 1678
MalI	GATC	9 cut(s)	486, 684, 952, 978, 1481, 1653, 1871, 1995, 2088
MboI	GATC	9 cut(s)	484, 682, 950, 976, 1479, 1651, 1869, 1993, 2086
MflI	RGATCY	3 cut(s)	976, 1993, 2086
MhlI	GDGCHC	1 cut(s)	1714
MlsI	TGGCCA	1 cut(s)	745
MluCI	AATT	7 cut(s)	35, 506, 867, 967, 1210, 1777, 2029
MluNI	TGGCCA	1 cut(s)	745
MlyI	GAGTC	5 cut(s)	178, 206, 575, 829, 1746
MmeI	TCCRAC	3 cut(s)	1004, 1714, 1917
Mox20I	TGGCCA	1 cut(s)	745
MscI	TGGCCA	1 cut(s)	745
MslI	CAYNNNNRTG	1 cut(s)	1520
Msp20I	TGGCCA	1 cut(s)	745
MspA1I	CMGCKG	3 cut(s)	560, 893, 1899
MspCI	CTTAAG	2 cut(s)	434, 2015
MspI	CCGG	3 cut(s)	138, 1326, 1688
MspR9I	CCNGG	6 cut(s)	270, 738, 1326, 1688, 1689, 1988
MvaI	CCWGG	3 cut(s)	270, 738, 1988
MvnI	CGCG	2 cut(s)	579, 1493
MwoI	GCNNNNNNNGC	5 cut(s)	470, 998, 1253, 1490, 1703
NciI	CCSGG	3 cut(s)	1326, 1688, 1689
NcoI	CCATGG	2 cut(s)	972, 1528
NdeII	GATC	9 cut(s)	484, 682, 950, 976, 1479, 1651, 1869, 1993, 2086
NlaIII	CATG	7 cut(s)	156, 245, 976, 1183, 1406, 1519, 1532
NlaIV	GGNNCC	3 cut(s)	785, 978, 1540
NmuCI	GTSAC	1 cut(s)	719
NspV	TTCGAA	1 cut(s)	751
PaeR7I	CTCGAG	1 cut(s)	788
PagI	TCATGA	1 cut(s)	1402
PaqCI	CACCTGC	1 cut(s)	1431
PcsI	WCGNNNNNNNCGW	1 cut(s)	946
PinAI	ACCGGT	1 cut(s)	137
PkrI	GCNGC	5 cut(s)	562, 1255, 1495, 1878, 2026
PleI	GAGTC	5 cut(s)	178, 205, 575, 828, 1745
PpsI	GAGTC	5 cut(s)	178, 205, 575, 828, 1745
PshAI	GACNNNNGTC	1 cut(s)	717
Psp124BI	GAGCTC	1 cut(s)	1714
Psp6I	CCWGG	3 cut(s)	268, 736, 1986
PspEI	GGTNACC	1 cut(s)	1446
PspGI	CCWGG	3 cut(s)	268, 736, 1986
PspN4I	GGNNCC	3 cut(s)	785, 978, 1540
PspPI	GGNCC	2 cut(s)	149, 1538
PsuI	RGATCY	3 cut(s)	976, 1993, 2086
PvuII	CAGCTG	2 cut(s)	893, 1899
RsaI	GTAC	7 cut(s)	235, 480, 903, 945, 1099, 1123, 1903
RsaNI	GTAC	7 cut(s)	234, 479, 902, 944, 1098, 1122, 1902
RseI	CAYNNNNRTG	1 cut(s)	1520
SacI	GAGCTC	1 cut(s)	1714
SalI	GTCGAC	1 cut(s)	59
SatI	GCNGC	5 cut(s)	561, 1254, 1494, 1877, 2025
Sau3AI	GATC	9 cut(s)	484, 682, 950, 976, 1479, 1651, 1869, 1993, 2086
Sau96I	GGNCC	2 cut(s)	149, 1538
ScaI	AGTACT	1 cut(s)	235
SchI	GAGTC	5 cut(s)	178, 206, 575, 829, 1746
ScrFI	CCNGG	6 cut(s)	270, 738, 1326, 1688, 1689, 1988
SduI	GDGCHC	1 cut(s)	1714
SfaNI	GCATC	2 cut(s)	1311, 1396
SfcI	CTRYAG	2 cut(s)	811, 2082
Sfr274I	CTCGAG	1 cut(s)	788
SfuI	TTCGAA	1 cut(s)	751
SgrDI	CGTCGACG	1 cut(s)	59
SinI	GGWCC	1 cut(s)	149
SlaI	CTCGAG	1 cut(s)	788
SmaI	CCCGGG	1 cut(s)	1689
SmiMI	CAYNNNNRTG	1 cut(s)	1520
SmlI	CTYRAG	5 cut(s)	434, 775, 788, 1786, 2015
SmoI	CTYRAG	5 cut(s)	434, 775, 788, 1786, 2015
SpeI	ACTAGT	2 cut(s)	236, 1295
Sse9I	AATT	7 cut(s)	35, 506, 867, 967, 1210, 1777, 2029
SsiI	CCGC	5 cut(s)	558, 1144, 1409, 1493, 1905
SspMI	CTAG	9 cut(s)	231, 237, 642, 729, 1226, 1296, 1341, 1661, 1694
SstI	GAGCTC	1 cut(s)	1714
StyD4I	CCNGG	6 cut(s)	268, 736, 1324, 1686, 1687, 1986
StyI	CCWWGG	3 cut(s)	676, 972, 1528
TaaI	ACNGT	9 cut(s)	53, 295, 796, 901, 1044, 1452, 1678, 1921, 1952
TaiI	ACGT	2 cut(s)	943, 1626
TaqI	TCGA	9 cut(s)	60, 99, 114, 751, 789, 1005, 1654, 1781, 2043
TasI	AATT	7 cut(s)	35, 506, 867, 967, 1210, 1777, 2029
TatI	WGTACW	2 cut(s)	233, 478
TauI	GCSGC	1 cut(s)	1496
TscAI	CASTG	3 cut(s)	771, 918, 1681
TseFI	GTSAC	1 cut(s)	719
TseI	GCWGC	4 cut(s)	560, 1253, 1876, 2024
Tsp45I	GTSAC	1 cut(s)	719
TspDTI	ATGAA	4 cut(s)	228, 867, 1945, 2022
TspGWI	ACGGA	1 cut(s)	46
TspMI	CCCGGG	1 cut(s)	1687
TspRI	CASTG	3 cut(s)	771, 918, 1681
Vha464I	CTTAAG	2 cut(s)	434, 2015
VpaK11BI	GGWCC	1 cut(s)	149
XagI	CCTNNNNNAGG	1 cut(s)	1704
XbaI	TCTAGA	1 cut(s)	1660
XhoI	CTCGAG	1 cut(s)	788
XmaI	CCCGGG	1 cut(s)	1687
XmiI	GTMKAC	1 cut(s)	60
XspI	CTAG	9 cut(s)	231, 237, 642, 729, 1226, 1296, 1341, 1661, 1694
ZrmI	AGTACT	1 cut(s)	235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.