Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0012217

Physical Location & Seq

Genomic Coordinates Forward (+)

8122552 .. 8126573

Gene Structure 4022 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031768

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2028 bp

ATGTGGAGTGATCCTATTGCAGGGTTCTATGCGCCGTGCTCACATTCTCAGGTATCAAATCATTTGACTCTTCTAGCTGAGTCTTTGCCTTCGGATTCGAATGAAGAATCGAGTGAACCAGGAGTGATTCGGGGAAATAGGAATAGATGCTCCGTTCCCGGTATTCTTTACAACACCAATACGGTAGAAGGCTTTCGTGCACTTGACAAACAGGAGTTGATTAAAGCAGAAGCGAAAAAGATTTGGGAGGACATTCAGAATGGAAAAGCTTTGGAGGATAGTTCTGTACTTTCCAGGTTCCTCCTTATCTCTTTTGCAGACCTCAAAAAATGGAGCTTTCATTACTGGTTTGCCTTCCCTGCTCTGGTGCTTGATCCTCCAGTAACATTGCTTGATTTGAAACCAGCTTCACAATTTTTTAGTATGAAGGAGGCAGAATCTGTCTCAGCAGTATTAAATGACTGGCGCGACTCAAGCTTAACGACAGATGTGCCATTCTTTTTGGTTGTAATTGATCCAAATTCGCATGCTACCATTAAGCATCTAAAGGACTGGGAAACTTGTCAGAGAGCTACTGATCACAAGTTGCTATTCGGTTTTTACGACCAATGCCATCTTCCAAAGAATCCTGGTTGGCCACTTCGGAACTTGCTTGCACTTATTTGCTCAAGATGGGATCTCAAGTCTGTTCGCTTTCTATGCTATAGAGAGACTCGTGGTTTCGCAGATCTAGGATTGTCCCTTGTTGGTGAAGCCCAGGTTACAGTTCCCCAAGGATGGGGAGATCTTCATAATACACCCAATGCAGTGGGGTGGGAACTTAACAAAGGGAAAAAAATACCCAGGTGTATTAGCCTTGCTAAATCTATGGATCCAACTAGGTTGGCCGTATCTGCTGCAGATTTGAATTTGAAACTAATGAGATGGCGTGCTTTACCTTCTCTCAACTTAAATACCTTGTCCTCTCTCAAGTGTCTCCTCCTTGGAGCAGGTACACTTGGATGCCAGGTTGCTCGCATGCTTATGGCTTGGGGTGTTCGGAAAATTACGTTGGTTGACAATGGCAGAGTGGCTATGTCTAACCCCTTGAGGCAGTCCCTGTATACATTAGATGACTGCCTAAATGGTGGTGAATTTAAAGCTGTAGCAGCAGTTAACAGTCTCAAGAAAATATTTCCCGCTGTGGAAGCGGAGGGTGTTGTCATGGCTATACCAATGCCTGGACATCCCGTTCCTAGTCAAGAAGAGCAGAGTGTGCTTGATGAGTGTAGGCGCCTACATGATTTAATTGATTCTCATGATGCAGTTTTCTTGTTAACTGATACAAGAGAAAGTCGGTGGCTTCCGTCTCTTCTTTGTGCCAATACTAACAAGATTACTATAACTGCAGCTCTTGGGTTTGATAGCTTTTTAGTTATGCGCCATGGAGCAGGTCCATGCAGCTCTAGCCATGACTCAAAATCTGAAGCTGCAAATGCTTTATCCACTGATCTGAACAGCCTTGCACTGATTGATAGAAGTGGAGGGCACAGATTGGGTTGCTACTTCTGCAATGATGTGGTTGCACCTATTGACTCAACCGCCAACCGCACCTTGGACCAGCAATGTACTGTTACACGCCCAGGCCTCGCTCCTATTGCCTCAGCTCTTGCAGTTGAGCTCTTAGTTGGGATCCTCCATCACCCTGATGGGGTATTTGCCGAAGGTGAAATTGCAAGCTCTAGTAATAATGGAAGCAGCGAGCAACCTCTTGGTATTTTACCCCATCAAATCCGTGGTTCCCTTGGACAGTTTTCACAGATGACACTTGTGGGCCACTCCTCAGACAGTTGCACGGCTTGCTGCAGCATCGTGGTGTCTGAATATCGAAAAAGGGGAACGGAATTTATACTACAAGCAATCAACCATCCCACCTATCTGGAGGATGTGACTGGACTAACAGAGTTGATGAAAACAGCTCATGCATTTCAAATGGATTGGGATGATGGGACTGATAATGATGATGATGATGATTGTGTTGAAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000407 GO:0000422 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006497 GO:0006501 GO:0006807 GO:0006914 GO:0006950 GO:0006952 GO:0006995 GO:0006996 GO:0007005 GO:0007033 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008641 GO:0009056 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0009991 GO:0010150 GO:0016043 GO:0016236 GO:0016237 GO:0016740 GO:0016874 GO:0016877 GO:0018410 GO:0019538 GO:0019778 GO:0019779 GO:0022411 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0034645 GO:0034727 GO:0036211 GO:0042157 GO:0042158 GO:0042594 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043687 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044804 GO:0044805 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061726 GO:0061919 GO:0070925 GO:0071496 GO:0071704 GO:0071840 GO:0090693 GO:0098542 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1903008 GO:1905037

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 ko04216 map04136 map04138 map04140 map04216

Pfam Domains

Protein Families

ATG7_N ThiF

Protein Analysis

676

Amino Acids

74.12

Weight (kDa)

5.51

Isoelectric Point (pI)

39.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG7_N	PF16420	5 - 290	2e-84	Ubiquitin-like modifier-activating enzyme ATG7 N-terminus
ThiF	PF00899	307 - 563	7.8e-38	ThiF family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45900
fragaria_vesca	FvH4_3g07910
malus_domestica	MD05G1302300.v1.1 MD10G1281000.v1.1
prunus_persica	Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1
pyrus_communis	pycom05g27990 pycom10g23460
rosa_chinensis	RchiOBHm_Chr5g0010641
rosa_laevigata	RLG00000031768
rosa_multiflora	Rmu_sc0006103.1_g000002 Rmu_sc0014635.1_g000003
rosa_roxburghii	Rroxscaffold_1G00065730
rosa_rugosa	Rorug04G0451400.1
rosa_samantha	Rh5AG083000 Rh5CG091000
rosa_wichuraiana	Rw0G000680 Rw0G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	980, 1421
AccB1I	GGYRCC	1 cut(s)	1272
AccB7I	CCANNNNNTGG	1 cut(s)	1220
AccI	GTMKAC	1 cut(s)	1103
AccII	CGCG	1 cut(s)	468
AciI	CCGC	4 cut(s)	1179, 1190, 1581, 1588
AclWI	GGATC	8 cut(s)	5, 368, 509, 684, 866, 879, 1666, 1679
AcoI	YGGCCR	2 cut(s)	635, 885
AcsI	RAATTY	5 cut(s)	520, 907, 1133, 1883, 2022
AcuI	CTGAAG	1 cut(s)	1485
AcyI	GRCGYC	1 cut(s)	1273
AfaI	GTAC	3 cut(s)	288, 994, 1609
AfiI	CCNNNNNNNGG	7 cut(s)	20, 364, 777, 778, 1220, 1594, 1690
AgsI	TTSAA	5 cut(s)	400, 907, 913, 1970, 2021
AjnI	CCWGG	8 cut(s)	118, 293, 628, 756, 842, 1005, 1219, 1621
AjuI	GAANNNNNNNTTGG	4 cut(s)	600, 632, 1034, 1066
AloI	GAACNNNNNNTCC	2 cut(s)	1215, 1247
Alw21I	GWGCWC	3 cut(s)	41, 202, 1662
Alw26I	GTCTC	5 cut(s)	448, 704, 980, 1166, 1353
Alw44I	GTGCAC	1 cut(s)	198
AlwI	GGATC	8 cut(s)	5, 368, 509, 684, 866, 879, 1666, 1679
AlwNI	CAGNNNCTG	2 cut(s)	440, 1099
AoxI	GGCC	4 cut(s)	635, 885, 1624, 1813
ApaLI	GTGCAC	1 cut(s)	198
ApeKI	GCWGC	8 cut(s)	896, 1148, 1388, 1440, 1469, 1737, 1842, 1845
ApoI	RAATTY	5 cut(s)	520, 907, 1133, 1883, 2022
Asp700I	GAANNNNTTC	1 cut(s)	192
AspLEI	GCGC	4 cut(s)	34, 468, 1275, 1422
AspS9I	GGNCC	3 cut(s)	1433, 1597, 1813
AsuC2I	CCSGG	1 cut(s)	159
AsuHPI	GGTGA	4 cut(s)	761, 1142, 1673, 1718
AsuII	TTCGAA	1 cut(s)	98
AvaII	GGWCC	2 cut(s)	1433, 1597
BaeGI	GKGCMC	2 cut(s)	202, 1530
BalI	TGGCCA	1 cut(s)	637
BamHI	GGATCC	2 cut(s)	871, 1671
BanI	GGYRCC	1 cut(s)	1272
BanII	GRGCYC	1 cut(s)	1662
BauI	CACGAG	1 cut(s)	714
Bbv12I	GWGCWC	3 cut(s)	41, 202, 1662
BbvCI	CCTCAGC	1 cut(s)	1642
BbvI	GCAGC	8 cut(s)	883, 1160, 1400, 1452, 1456, 1749, 1829, 1857
BccI	CCATC	9 cut(s)	621, 666, 771, 918, 1682, 1686, 1773, 1914, 1979
BceAI	ACGGC	3 cut(s)	19, 872, 1851
BcgI	CGANNNNNNTGC	2 cut(s)	472, 506
BciT130I	CCWGG	8 cut(s)	120, 295, 630, 758, 844, 1007, 1221, 1623
BclI	TGATCA	1 cut(s)	577
BcnI	CCSGG	1 cut(s)	159
BcoDI	GTCTC	5 cut(s)	448, 704, 980, 1166, 1353
BfaI	CTAG	6 cut(s)	74, 731, 879, 1236, 1446, 1722
BfmI	CTRYAG	5 cut(s)	703, 897, 1143, 1386, 1843
BfoI	RGCGCY	1 cut(s)	1276
BfuAI	ACCTGC	2 cut(s)	980, 1421
BglII	AGATCT	2 cut(s)	727, 784
BisI	GCNGC	8 cut(s)	897, 1149, 1389, 1441, 1470, 1738, 1843, 1846
BlsI	GCNGC	8 cut(s)	898, 1150, 1390, 1442, 1471, 1739, 1844, 1847
Bme1390I	CCNGG	9 cut(s)	120, 159, 295, 630, 758, 844, 1007, 1221, 1623
Bme18I	GGWCC	2 cut(s)	1433, 1597
BmgT120I	GGNCC	3 cut(s)	1433, 1597, 1813
BmiI	GGNNCC	5 cut(s)	299, 873, 1274, 1673, 1780
BmrFI	CCNGG	9 cut(s)	120, 159, 295, 630, 758, 844, 1007, 1221, 1623
BmrI	ACTGGG	1 cut(s)	562
BmsI	GCATC	5 cut(s)	137, 550, 992, 1291, 1857
BmuI	ACTGGG	1 cut(s)	562
BpmI	CTGGAG	2 cut(s)	363, 1940
Bpu10I	CCTNAGC	1 cut(s)	1642
Bpu14I	TTCGAA	1 cut(s)	98
BpuEI	CTTGAG	6 cut(s)	457, 652, 665, 953, 1108, 1148
BpuMI	CCSGG	1 cut(s)	159
BsaHI	GRCGYC	1 cut(s)	1273
BsaJI	CCNNGG	9 cut(s)	756, 772, 842, 982, 1423, 1593, 1621, 1774, 1783
Bsc4I	CCNNNNNNNGG	7 cut(s)	20, 364, 777, 778, 1220, 1594, 1690
Bse1I	ACTGG	5 cut(s)	350, 380, 467, 557, 1936
Bse3DI	GCAATG	3 cut(s)	386, 1558, 1610
BseBI	CCWGG	8 cut(s)	120, 295, 630, 758, 844, 1007, 1221, 1623
BseDI	CCNNGG	9 cut(s)	756, 772, 842, 982, 1423, 1593, 1621, 1774, 1783
BseGI	GGATG	6 cut(s)	782, 1007, 1225, 1906, 1930, 1987
BseLI	CCNNNNNNNGG	7 cut(s)	20, 364, 777, 778, 1220, 1594, 1690
BseMI	GCAATG	3 cut(s)	386, 1558, 1610
BseMII	CTCAG	5 cut(s)	62, 69, 459, 1656, 1836
BseNI	ACTGG	5 cut(s)	350, 380, 467, 557, 1936
BseRI	GAGGAG	2 cut(s)	968, 1810
BseSI	GKGCMC	2 cut(s)	202, 1530
BseXI	GCAGC	8 cut(s)	883, 1160, 1400, 1452, 1456, 1749, 1829, 1857
Bsh1236I	CGCG	1 cut(s)	468
BshFI	GGCC	4 cut(s)	637, 887, 1626, 1815
BshNI	GGYRCC	1 cut(s)	1272
BsiHKAI	GWGCWC	3 cut(s)	41, 202, 1662
BsiSI	CCGG	1 cut(s)	159
BslFI	GGGAC	3 cut(s)	724, 1081, 2002
BslI	CCNNNNNNNGG	7 cut(s)	20, 364, 777, 778, 1220, 1594, 1690
BsmAI	GTCTC	5 cut(s)	448, 704, 980, 1166, 1353
BsmBI	CGTCTC	1 cut(s)	1353
BsmFI	GGGAC	3 cut(s)	724, 1081, 2002
BsnI	GGCC	4 cut(s)	637, 887, 1626, 1815
Bsp119I	TTCGAA	1 cut(s)	98
Bsp1286I	GDGCHC	4 cut(s)	41, 202, 1530, 1662
Bsp19I	CCATGG	1 cut(s)	1423
BspACI	CCGC	4 cut(s)	1179, 1190, 1581, 1588
BspANI	GGCC	4 cut(s)	637, 887, 1626, 1815
BspCNI	CTCAG	5 cut(s)	61, 70, 458, 1655, 1835
BspFNI	CGCG	1 cut(s)	468
BspHI	TCATGA	1 cut(s)	1297
BspLI	GGNNCC	5 cut(s)	299, 873, 1274, 1673, 1780
BspMAI	CTGCAG	3 cut(s)	901, 1390, 1847
BspMI	ACCTGC	2 cut(s)	980, 1421
BspPI	GGATC	8 cut(s)	5, 368, 509, 684, 866, 879, 1666, 1679
BspQI	GCTCTTC	1 cut(s)	1239
BspT104I	TTCGAA	1 cut(s)	98
BspT107I	GGYRCC	1 cut(s)	1272
BsrDI	GCAATG	3 cut(s)	386, 1558, 1610
BsrI	ACTGG	5 cut(s)	350, 380, 467, 557, 1936
BssECI	CCNNGG	9 cut(s)	756, 772, 842, 982, 1423, 1593, 1621, 1774, 1783
BssNAI	GTATAC	1 cut(s)	1104
BssNI	GRCGYC	1 cut(s)	1273
BssSI	CACGAG	1 cut(s)	714
BssT1I	CCWWGG	5 cut(s)	772, 982, 1423, 1593, 1783
Bst1107I	GTATAC	1 cut(s)	1104
Bst2BI	CACGAG	1 cut(s)	714
Bst2UI	CCWGG	8 cut(s)	120, 295, 630, 758, 844, 1007, 1221, 1623
Bst4CI	ACNGT	6 cut(s)	184, 766, 1160, 1612, 1791, 1829
Bst6I	CTCTTC	3 cut(s)	75, 1239, 1356
BstACI	GRCGYC	1 cut(s)	1273
BstAPI	GCANNNNNTGC	2 cut(s)	1255, 1839
BstBI	TTCGAA	1 cut(s)	98
BstC8I	GCNNGC	8 cut(s)	528, 654, 930, 1015, 1019, 1717, 1742, 1840
BstDEI	CTNAG	6 cut(s)	48, 78, 445, 1642, 1663, 1822
BstDSI	CCRYGG	2 cut(s)	1423, 1774
BstENI	CCTNNNNNAGG	1 cut(s)	18
BstF5I	GGATG	6 cut(s)	782, 1007, 1225, 1906, 1930, 1987
BstFNI	CGCG	1 cut(s)	468
BstH2I	RGCGCY	1 cut(s)	1276
BstHHI	GCGC	4 cut(s)	34, 468, 1275, 1422
BstMAI	GTCTC	5 cut(s)	448, 704, 980, 1166, 1353
BstNI	CCWGG	8 cut(s)	120, 295, 630, 758, 844, 1007, 1221, 1623
BstNSI	RCATGY	2 cut(s)	530, 1021
BstSCI	CCNGG	9 cut(s)	118, 157, 293, 628, 756, 842, 1005, 1219, 1621
BstSFI	CTRYAG	5 cut(s)	703, 897, 1143, 1386, 1843
BstSLI	GKGCMC	2 cut(s)	202, 1530
BstUI	CGCG	1 cut(s)	468
BstV1I	GCAGC	8 cut(s)	883, 1160, 1400, 1452, 1456, 1749, 1829, 1857
BstX2I	RGATCY	5 cut(s)	676, 727, 784, 871, 1671
BstXI	CCANNNNNNTGG	2 cut(s)	808, 1918
BstYI	RGATCY	5 cut(s)	676, 727, 784, 871, 1671
BstZ17I	GTATAC	1 cut(s)	1104
BsuRI	GGCC	4 cut(s)	637, 887, 1626, 1815
BtgI	CCRYGG	2 cut(s)	1423, 1774
BtsCI	GGATG	6 cut(s)	782, 1007, 1225, 1906, 1930, 1987
BtsI	GCAGTG	1 cut(s)	813
BtsIMutI	CAGTG	3 cut(s)	813, 1485, 1505
BveI	ACCTGC	2 cut(s)	980, 1421
Cac8I	GCNNGC	8 cut(s)	528, 654, 930, 1015, 1019, 1717, 1742, 1840
CaiI	CAGNNNCTG	2 cut(s)	440, 1099
CciI	TCATGA	1 cut(s)	1297
CfoI	GCGC	4 cut(s)	34, 468, 1275, 1422
Cfr13I	GGNCC	3 cut(s)	1433, 1597, 1813
Csp6I	GTAC	3 cut(s)	287, 993, 1608
CviAII	CATG	9 cut(s)	527, 1018, 1204, 1280, 1298, 1424, 1437, 1451, 1961
CviQI	GTAC	3 cut(s)	287, 993, 1608
DdeI	CTNAG	6 cut(s)	48, 78, 445, 1642, 1663, 1822
DinI	GGCGCC	1 cut(s)	1274
DraI	TTTAAA	1 cut(s)	1138
EaeI	YGGCCR	2 cut(s)	635, 885
Eam1104I	CTCTTC	3 cut(s)	75, 1239, 1356
EarI	CTCTTC	3 cut(s)	75, 1239, 1356
Ecl136II	GAGCTC	1 cut(s)	1660
Eco130I	CCWWGG	5 cut(s)	772, 982, 1423, 1593, 1783
Eco147I	AGGCCT	1 cut(s)	1626
Eco24I	GRGCYC	1 cut(s)	1662
Eco47I	GGWCC	2 cut(s)	1433, 1597
Eco53kI	GAGCTC	1 cut(s)	1660
Eco57I	CTGAAG	1 cut(s)	1485
EcoICRI	GAGCTC	1 cut(s)	1660
EcoNI	CCTNNNNNAGG	1 cut(s)	18
EcoRII	CCWGG	8 cut(s)	118, 293, 628, 756, 842, 1005, 1219, 1621
EcoT14I	CCWWGG	5 cut(s)	772, 982, 1423, 1593, 1783
EcoT22I	ATGCAT	1 cut(s)	1966
EcoT38I	GRGCYC	1 cut(s)	1662
EgeI	GGCGCC	1 cut(s)	1274
EheI	GGCGCC	1 cut(s)	1274
ErhI	CCWWGG	5 cut(s)	772, 982, 1423, 1593, 1783
Esp3I	CGTCTC	1 cut(s)	1353
FaeI	CATG	9 cut(s)	530, 1021, 1207, 1283, 1301, 1427, 1440, 1454, 1964
FaqI	GGGAC	3 cut(s)	724, 1081, 2002
FatI	CATG	9 cut(s)	526, 1017, 1203, 1279, 1297, 1423, 1436, 1450, 1960
FauI	CCCGC	1 cut(s)	1186
FbaI	TGATCA	1 cut(s)	577
FblI	GTMKAC	1 cut(s)	1103
Fnu4HI	GCNGC	8 cut(s)	897, 1149, 1389, 1441, 1470, 1738, 1843, 1846
FokI	GGATG	6 cut(s)	789, 1014, 1212, 1893, 1937, 1994
FriOI	GRGCYC	1 cut(s)	1662
Fsp4HI	GCNGC	8 cut(s)	897, 1149, 1389, 1441, 1470, 1738, 1843, 1846
FspBI	CTAG	6 cut(s)	74, 731, 879, 1236, 1446, 1722
GlaI	GCGC	4 cut(s)	33, 467, 1274, 1421
GluI	GCNGC	8 cut(s)	897, 1149, 1389, 1441, 1470, 1738, 1843, 1846
GsuI	CTGGAG	2 cut(s)	363, 1940
HaeII	RGCGCY	1 cut(s)	1276
HaeIII	GGCC	4 cut(s)	637, 887, 1626, 1815
HapII	CCGG	1 cut(s)	159
HhaI	GCGC	4 cut(s)	34, 468, 1275, 1422
Hin1I	GRCGYC	1 cut(s)	1273
Hin1II	CATG	9 cut(s)	530, 1021, 1207, 1283, 1301, 1427, 1440, 1454, 1964
Hin6I	GCGC	4 cut(s)	32, 466, 1273, 1420
HinP1I	GCGC	4 cut(s)	32, 466, 1273, 1420
HincII	GTYRAC	3 cut(s)	1057, 1156, 1317
HindII	GTYRAC	3 cut(s)	1057, 1156, 1317
HindIII	AAGCTT	2 cut(s)	267, 475
HpaI	GTTAAC	2 cut(s)	1156, 1317
HpaII	CCGG	1 cut(s)	159
HphI	GGTGA	4 cut(s)	761, 1142, 1673, 1718
Hpy166II	GTNNAC	7 cut(s)	116, 200, 995, 1057, 1104, 1156, 1317
Hpy188I	TCNGA	9 cut(s)	94, 258, 567, 645, 1041, 1465, 1494, 1825, 1861
Hpy188III	TCNNGA	5 cut(s)	669, 1165, 1241, 1298, 1919
Hpy8I	GTNNAC	7 cut(s)	116, 200, 995, 1057, 1104, 1156, 1317
HpyAV	CCTTC	6 cut(s)	99, 182, 364, 421, 948, 1697
HpyCH4III	ACNGT	6 cut(s)	184, 766, 1160, 1612, 1791, 1829
HpyCH4IV	ACGT	1 cut(s)	1049
HpyF3I	CTNAG	6 cut(s)	48, 78, 445, 1642, 1663, 1822
HpySE526I	ACGT	1 cut(s)	1049
Hsp92I	GRCGYC	1 cut(s)	1273
Hsp92II	CATG	9 cut(s)	530, 1021, 1207, 1283, 1301, 1427, 1440, 1454, 1964
HspAI	GCGC	4 cut(s)	32, 466, 1273, 1420
KasI	GGCGCC	1 cut(s)	1272
Ksp22I	TGATCA	1 cut(s)	577
KspAI	GTTAAC	2 cut(s)	1156, 1317
LguI	GCTCTTC	1 cut(s)	1239
LmnI	GCTCC	5 cut(s)	155, 333, 986, 1427, 1636
Lsp1109I	GCAGC	8 cut(s)	883, 1160, 1400, 1452, 1456, 1749, 1829, 1857
LweI	GCATC	5 cut(s)	137, 550, 992, 1291, 1857
MaeI	CTAG	6 cut(s)	74, 731, 879, 1236, 1446, 1722
MaeII	ACGT	1 cut(s)	1049
MaeIII	GTNAC	4 cut(s)	382, 760, 1612, 1927
MboII	GAAGA	6 cut(s)	62, 116, 608, 779, 1256, 1343
MflI	RGATCY	5 cut(s)	676, 727, 784, 871, 1671
MhlI	GDGCHC	4 cut(s)	41, 202, 1530, 1662
MlsI	TGGCCA	1 cut(s)	637
MluNI	TGGCCA	1 cut(s)	637
Mly113I	GGCGCC	1 cut(s)	1273
MlyI	GAGTC	6 cut(s)	61, 89, 464, 706, 1448, 1568
MmeI	TCCRAC	1 cut(s)	899
Mox20I	TGGCCA	1 cut(s)	637
Mph1103I	ATGCAT	1 cut(s)	1966
MroXI	GAANNNNTTC	1 cut(s)	192
MscI	TGGCCA	1 cut(s)	637
MslI	CAYNNNNRTG	4 cut(s)	1000, 1022, 1686, 1853
Msp20I	TGGCCA	1 cut(s)	637
MspA1I	CMGCKG	1 cut(s)	1181
MspI	CCGG	1 cut(s)	159
MspR9I	CCNGG	9 cut(s)	120, 159, 295, 630, 758, 844, 1007, 1221, 1623
MvaI	CCWGG	8 cut(s)	120, 295, 630, 758, 844, 1007, 1221, 1623
MvnI	CGCG	1 cut(s)	468
NarI	GGCGCC	1 cut(s)	1273
NciI	CCSGG	1 cut(s)	159
NcoI	CCATGG	1 cut(s)	1423
NlaIII	CATG	9 cut(s)	530, 1021, 1207, 1283, 1301, 1427, 1440, 1454, 1964
NlaIV	GGNNCC	5 cut(s)	299, 873, 1274, 1673, 1780
NmuCI	GTSAC	1 cut(s)	1927
NsiI	ATGCAT	1 cut(s)	1966
NspI	RCATGY	2 cut(s)	530, 1021
NspV	TTCGAA	1 cut(s)	98
PaeI	GCATGC	2 cut(s)	530, 1021
PagI	TCATGA	1 cut(s)	1297
PceI	AGGCCT	1 cut(s)	1626
PciSI	GCTCTTC	1 cut(s)	1239
PcsI	WCGNNNNNNNCGW	1 cut(s)	600
PdmI	GAANNNNTTC	1 cut(s)	192
PfeI	GAWTC	6 cut(s)	95, 107, 127, 437, 625, 1292
PflMI	CCANNNNNTGG	1 cut(s)	1220
PkrI	GCNGC	8 cut(s)	898, 1150, 1390, 1442, 1471, 1739, 1844, 1847
PleI	GAGTC	6 cut(s)	61, 88, 464, 706, 1448, 1568
PluTI	GGCGCC	1 cut(s)	1276
PpsI	GAGTC	6 cut(s)	61, 88, 464, 706, 1448, 1568
Psp124BI	GAGCTC	1 cut(s)	1662
Psp6I	CCWGG	8 cut(s)	118, 293, 628, 756, 842, 1005, 1219, 1621
PspGI	CCWGG	8 cut(s)	118, 293, 628, 756, 842, 1005, 1219, 1621
PspN4I	GGNNCC	5 cut(s)	299, 873, 1274, 1673, 1780
PspPI	GGNCC	3 cut(s)	1433, 1597, 1813
PstI	CTGCAG	3 cut(s)	901, 1390, 1847
PstNI	CAGNNNCTG	2 cut(s)	440, 1099
PsuI	RGATCY	5 cut(s)	676, 727, 784, 871, 1671
RsaI	GTAC	3 cut(s)	288, 994, 1609
RsaNI	GTAC	3 cut(s)	287, 993, 1608
RseI	CAYNNNNRTG	4 cut(s)	1000, 1022, 1686, 1853
SacI	GAGCTC	1 cut(s)	1662
SapI	GCTCTTC	1 cut(s)	1239
SatI	GCNGC	8 cut(s)	897, 1149, 1389, 1441, 1470, 1738, 1843, 1846
Sau96I	GGNCC	3 cut(s)	1433, 1597, 1813
SchI	GAGTC	6 cut(s)	61, 89, 464, 706, 1448, 1568
ScrFI	CCNGG	9 cut(s)	120, 159, 295, 630, 758, 844, 1007, 1221, 1623
SduI	GDGCHC	4 cut(s)	41, 202, 1530, 1662
SfaNI	GCATC	5 cut(s)	137, 550, 992, 1291, 1857
SfcI	CTRYAG	5 cut(s)	703, 897, 1143, 1386, 1843
SfoI	GGCGCC	1 cut(s)	1274
SfuI	TTCGAA	1 cut(s)	98
SinI	GGWCC	2 cut(s)	1433, 1597
SmiMI	CAYNNNNRTG	4 cut(s)	1000, 1022, 1686, 1853
SmlI	CTYRAG	6 cut(s)	472, 667, 680, 968, 1087, 1163
SmoI	CTYRAG	6 cut(s)	472, 667, 680, 968, 1087, 1163
SphI	GCATGC	2 cut(s)	530, 1021
SseBI	AGGCCT	1 cut(s)	1626
SsiI	CCGC	4 cut(s)	1179, 1190, 1581, 1588
SspDI	GGCGCC	1 cut(s)	1272
SspI	AATATT	1 cut(s)	1173
SspMI	CTAG	6 cut(s)	74, 731, 879, 1236, 1446, 1722
SstI	GAGCTC	1 cut(s)	1662
StuI	AGGCCT	1 cut(s)	1626
StyD4I	CCNGG	9 cut(s)	118, 157, 293, 628, 756, 842, 1005, 1219, 1621
StyI	CCWWGG	5 cut(s)	772, 982, 1423, 1593, 1783
TaaI	ACNGT	6 cut(s)	184, 766, 1160, 1612, 1791, 1829
TaiI	ACGT	1 cut(s)	1052
TaqI	TCGA	3 cut(s)	98, 110, 1867
TatI	WGTACW	2 cut(s)	286, 1607
TfiI	GAWTC	6 cut(s)	95, 107, 127, 437, 625, 1292
TscAI	CASTG	3 cut(s)	813, 1492, 1512
TseFI	GTSAC	1 cut(s)	1927
TseI	GCWGC	8 cut(s)	896, 1148, 1388, 1440, 1469, 1737, 1842, 1845
Tsp45I	GTSAC	1 cut(s)	1927
TspDTI	ATGAA	5 cut(s)	117, 329, 440, 779, 1964
TspGWI	ACGGA	4 cut(s)	142, 1335, 1763, 1895
TspRI	CASTG	3 cut(s)	813, 1492, 1512
Van91I	CCANNNNNTGG	1 cut(s)	1220
VneI	GTGCAC	1 cut(s)	198
VpaK11BI	GGWCC	2 cut(s)	1433, 1597
XagI	CCTNNNNNAGG	1 cut(s)	18
XapI	RAATTY	5 cut(s)	520, 907, 1133, 1883, 2022
XceI	RCATGY	2 cut(s)	530, 1021
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1591, 1685, 1772
XmiI	GTMKAC	1 cut(s)	1103
XmnI	GAANNNNTTC	1 cut(s)	192
XspI	CTAG	6 cut(s)	74, 731, 879, 1236, 1446, 1722
Zsp2I	ATGCAT	1 cut(s)	1966

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.