Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012217

Physical Location & Seq

Genomic Coordinates Reverse (-)

37175655 .. 37179925

Gene Structure 4271 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1281000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGGAGCGAGGCAGCGGGAAATCCATACTCCAATTCGCACCGTTTCAGAGCTCCGTCGACGAGGGTTTCTGGCACAGACTCTCTTCCTTGAAGCTCAACAAGTTCGGCATCGACGACTCGCCTGTTCCCATCACCGGTTTCTACGCACCGTGCTCGCATTCTCAAGTGTCAAATCATTTAACTCTTTTAGCTGAGTCTTTGCCTTCGGATTCGAGTGAGGAATCATCGGTAACAGGAGTAAGTCGGGGAAATAGGAACAGATGCTCCGTTCCGGGAATCATTTACAACACTAATACGGTAGAAGGCTTTCATGCGCTTGATAAACAAGGCTTGCTTAAGGCAGAAGCACAAAAGATTTGGAAGGACATCCAGAGTGGAAGAGCATTGGAGGATAGTTCAGTACTTTTGAGGTTCCTCGTCATCTCTTTTGCCGACCTGAAAAAATGGAGCTTTAATTACTGGTTTGCTTTCCCCGCTTTGGTGCTTGATCCTCCAGCAACTTTGGTAAATTTGAGGCCAGCTTCACAGTGTTTTAGCTCGGAGGAGGCAGAATCTGTTTCAGCAGCATTTAACGAGTGGCGTAACTCAAGCCTAACAGCAGACGTGCCATTCTTCTTGGTTCATCTTGATTCAAATTCACACGCTACTATTAAGCATCTAAAGGATTGGGAAACCTCCCAAAGTGCTGATGATGGTCACAAGTTACTCTTTGGTTTTTACGATCCATGCCATCTCCCAAACCATCCCGGTTGGCCTCTTCGCAACCTCCTTGCTCTTATTTGCTCAAGATGGGATCTAAAGTCTGTTCACTTTTTATGCTATAGAGAGAGTCGGGGTTTCGCAGATCTGGGACTGTCCCTTGTTGGTGAAGCTCTGATTACAGTTCCGCCAGGATGGAGAGATCATGGTCATGTACCAAATGCAGTCGGGTGGGAACTTAACAAAGGGAGAAAAGTACCCAGGATTATTAGCCTTGCTAAATCTATGGATCCAACTAGGTTGGCCATATCTGCTGCAGATTTGAATTTGAAACTAATGAGATGGCGTGCTTTGCCTTCTTTGAACTTAAATAGCTTGTCTTCTCTCAAGTGTCTCCTCCTTGGAGCTGGTACACTTGGATGCCAGGTTGCTCGGACGCTTATGGCTTGGGGTGTCCGGACCATTACGTTGGTTGACAATGGCAGGGTGGCTATGTCTAATCCGCTGAGGCAATCACTGTATACATTAGATGACTGCCTAAATGGCGGTGAATTTAAAGTGACAGCTGCAGTTAATAGTCTCAAGAAAATATTTCCAGCTGTGGAAGCAGAGGGTGTCATCATGGCTATACCTATGCCTGGACATCCTGTTCCGAGTCAAGAAGAGCAGAGCGTGCTCGATGATTGTGGACGACTACATGATTTAATTGATTGTCATGATGTAGTTTTCCTGCTGACTGATACAAGAGAAAGTCGGTGGCTACCATCTCTCCTTTGTGCAAATACTAACAAGATTACTATAACTGCGGCTTTAGGGTTTGATAGCTTCTTGGTTATGCGCCATGGAGCAGGTCCTTGTAGCTCTAGCCATGACTCAAAAACTGAAGCTGAAAATGCTTTATCTGCTTATATGGGCAATCTTGGCCTTACTGATAGAGACGGGGGGAAGAGATTGGGCTGTTACTTCTGCAATGATGTCGTTGCGCCTATTGATTCAACTTCAAACCGCACATTGGACCAGCAATGCACTGTTACACGCCCAGGCCTTGCTCCTATTGCCTCAGCTCTTGCGGTTGAGCTCTTAGTGGGGATCCTGCATCACCCTCATGGGATATTTGCGGAAGGGGAGGTGGTAAACTGCAGTAATAGCAGAAGCAGTGAGCAGCCTCTTGGTATTTTACCCCATCAGATTCGCGGTTCCCTAGCACAGTTTTCGCAGATGACACTTGTGGGCCACTCCTCAGACAGTTGCACGGCTTGTTGCAGCACTGTGGTGTCCGAATATCGGAAAAGGGGAATGGAATTTATTTTGCAAGCAATCAACCATCCTACATATCTGGAGGACCTAACTGGACTGACAGAGTTGATGAAATCAGCTAGTAAATTCGAGTTGGACTGGGACGACGGGACAGATGAAGACGATGATGATTTGGTTGAGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000407 GO:0000422 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006497 GO:0006501 GO:0006807 GO:0006914 GO:0006950 GO:0006952 GO:0006995 GO:0006996 GO:0007005 GO:0007033 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008641 GO:0009056 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0009991 GO:0010150 GO:0016043 GO:0016236 GO:0016237 GO:0016740 GO:0016874 GO:0016877 GO:0018410 GO:0019538 GO:0019778 GO:0019779 GO:0022411 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0034645 GO:0034727 GO:0036211 GO:0042157 GO:0042158 GO:0042594 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043687 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044804 GO:0044805 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061726 GO:0061919 GO:0070925 GO:0071496 GO:0071704 GO:0071840 GO:0090693 GO:0098542 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1903008 GO:1905037

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 ko04216 map04136 map04138 map04140 map04216

Pfam Domains

Protein Families

ATG7_N ThiF

Protein Analysis

714

Amino Acids

78.13

Weight (kDa)

5.68

Isoelectric Point (pI)

43.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG7_N	PF16420	10 - 329	4.4e-102	Ubiquitin-like modifier-activating enzyme ATG7 N-terminus
ThiF	PF00899	346 - 602	4.8e-36	ThiF family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45900
fragaria_vesca	FvH4_3g07910
malus_domestica	MD05G1302300.v1.1 MD10G1281000.v1.1
prunus_persica	Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1
pyrus_communis	pycom05g27990 pycom10g23460
rosa_chinensis	RchiOBHm_Chr5g0010641
rosa_laevigata	RLG00000031768
rosa_multiflora	Rmu_sc0006103.1_g000002 Rmu_sc0014635.1_g000003
rosa_roxburghii	Rroxscaffold_1G00065730
rosa_rugosa	Rorug04G0451400.1
rosa_samantha	Rh5AG083000 Rh5CG091000
rosa_wichuraiana	Rw0G000680 Rw0G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1538
AccI	GTMKAC	2 cut(s)	57, 1220
AccII	CGCG	1 cut(s)	1893
AccIII	TCCGGA	1 cut(s)	1155
AclWI	GGATC	7 cut(s)	482, 716, 801, 983, 996, 1783, 1796
AcoI	YGGCCR	1 cut(s)	1002
AcsI	RAATTY	6 cut(s)	508, 634, 1024, 1250, 2000, 2081
AcuI	CTGAAG	1 cut(s)	1602
AfaI	GTAC	4 cut(s)	402, 915, 957, 1111
AfiI	CCNNNNNNNGG	5 cut(s)	134, 478, 1337, 1711, 1983
AflII	CTTAAG	1 cut(s)	335
AgeI	ACCGGT	1 cut(s)	134
AgsI	TTSAA	7 cut(s)	91, 633, 1024, 1030, 1063, 1695, 1701
AjiI	CACGTC	1 cut(s)	604
AjnI	CCWGG	5 cut(s)	889, 959, 1122, 1336, 1738
AleI	CACNNNNGTG	1 cut(s)	1970
AloI	GAACNNNNNNTCC	4 cut(s)	248, 280, 1332, 1364
Alw21I	GWGCWC	4 cut(s)	53, 155, 1377, 1779
Alw26I	GTCTC	3 cut(s)	1097, 1283, 1629
AlwI	GGATC	7 cut(s)	482, 716, 801, 983, 996, 1783, 1796
AlwNI	CAGNNNCTG	1 cut(s)	554
Aor13HI	TCCGGA	1 cut(s)	1155
AoxI	GGCC	6 cut(s)	515, 752, 1002, 1621, 1741, 1930
ApeKI	GCWGC	6 cut(s)	12, 563, 1013, 1265, 1861, 1962
ApoI	RAATTY	6 cut(s)	508, 634, 1024, 1250, 2000, 2081
AsiGI	ACCGGT	1 cut(s)	134
Asp700I	GAANNNNTTC	1 cut(s)	306
AspLEI	GCGC	3 cut(s)	316, 1539, 1684
AspS9I	GGNCC	5 cut(s)	1158, 1550, 1714, 1930, 2041
AsuC2I	CCSGG	2 cut(s)	273, 747
AsuHPI	GGTGA	4 cut(s)	124, 878, 1259, 1790
AvaII	GGWCC	4 cut(s)	1158, 1550, 1714, 2041
BalI	TGGCCA	1 cut(s)	1004
BamHI	GGATCC	2 cut(s)	988, 1788
BanII	GRGCYC	2 cut(s)	53, 1779
BbsI	GAAGAC	2 cut(s)	1071, 2121
Bbv12I	GWGCWC	4 cut(s)	53, 155, 1377, 1779
BbvCI	CCTCAGC	2 cut(s)	1205, 1759
BbvI	GCAGC	6 cut(s)	24, 575, 1000, 1252, 1873, 1974
BceAI	ACGGC	1 cut(s)	1968
BcgI	CGANNNNNNTGC	6 cut(s)	135, 169, 1657, 1691, 1893, 1927
BciT130I	CCWGG	5 cut(s)	891, 961, 1124, 1338, 1740
BcnI	CCSGG	2 cut(s)	273, 747
BcoDI	GTCTC	3 cut(s)	1097, 1283, 1629
BfaI	CTAG	4 cut(s)	996, 1563, 1901, 2076
BfmI	CTRYAG	4 cut(s)	820, 1014, 1266, 1837
BfrI	CTTAAG	1 cut(s)	335
BfuAI	ACCTGC	1 cut(s)	1538
BglI	GCCNNNNNGGC	1 cut(s)	1242
BglII	AGATCT	1 cut(s)	844
BisI	GCNGC	7 cut(s)	13, 564, 1014, 1266, 1506, 1862, 1963
BlsI	GCNGC	7 cut(s)	14, 565, 1015, 1267, 1507, 1863, 1964
BmcAI	AGTACT	1 cut(s)	402
Bme1390I	CCNGG	7 cut(s)	273, 747, 891, 961, 1124, 1338, 1740
Bme18I	GGWCC	4 cut(s)	1158, 1550, 1714, 2041
BmgBI	CACGTC	1 cut(s)	604
BmgT120I	GGNCC	5 cut(s)	1158, 1550, 1714, 1930, 2041
BmiI	GGNNCC	4 cut(s)	413, 990, 1790, 1897
BmrFI	CCNGG	7 cut(s)	273, 747, 891, 961, 1124, 1338, 1740
BmrI	ACTGGG	1 cut(s)	2104
BmsI	GCATC	5 cut(s)	117, 251, 664, 1109, 1804
BmuI	ACTGGG	1 cut(s)	2104
BpiI	GAAGAC	2 cut(s)	1071, 2121
BpmI	CTGGAG	2 cut(s)	477, 2057
Bpu10I	CCTNAGC	2 cut(s)	1205, 1759
BpuEI	CTTGAG	5 cut(s)	147, 571, 769, 1070, 1265
BpuMI	CCSGG	2 cut(s)	273, 747
BsaJI	CCNNGG	4 cut(s)	959, 1099, 1540, 1738
BsaWI	WCCGGW	2 cut(s)	134, 1155
BsaXI	ACNNNNNCTCC	4 cut(s)	248, 278, 1452, 1482
Bsc4I	CCNNNNNNNGG	5 cut(s)	134, 478, 1337, 1711, 1983
Bse118I	RCCGGY	1 cut(s)	134
Bse1I	ACTGG	3 cut(s)	464, 2053, 2099
Bse3DI	GCAATG	2 cut(s)	1675, 1727
BseAI	TCCGGA	1 cut(s)	1155
BseBI	CCWGG	5 cut(s)	891, 961, 1124, 1338, 1740
BseDI	CCNNGG	4 cut(s)	959, 1099, 1540, 1738
BseGI	GGATG	6 cut(s)	366, 742, 899, 1124, 1342, 2023
BseLI	CCNNNNNNNGG	5 cut(s)	134, 478, 1337, 1711, 1983
BseMI	GCAATG	2 cut(s)	1675, 1727
BseMII	CTCAG	4 cut(s)	183, 1196, 1773, 1953
BseNI	ACTGG	3 cut(s)	464, 2053, 2099
BseRI	GAGGAG	3 cut(s)	557, 1085, 1927
BseXI	GCAGC	6 cut(s)	24, 575, 1000, 1252, 1873, 1974
Bsh1236I	CGCG	1 cut(s)	1893
BshFI	GGCC	6 cut(s)	517, 754, 1004, 1623, 1743, 1932
BshTI	ACCGGT	1 cut(s)	134
BsiHKAI	GWGCWC	4 cut(s)	53, 155, 1377, 1779
BsiSI	CCGG	4 cut(s)	135, 272, 747, 1156
BslFI	GGGAC	4 cut(s)	841, 864, 2111, 2119
BslI	CCNNNNNNNGG	5 cut(s)	134, 478, 1337, 1711, 1983
BsmAI	GTCTC	3 cut(s)	1097, 1283, 1629
BsmBI	CGTCTC	1 cut(s)	1629
BsmFI	GGGAC	4 cut(s)	841, 864, 2111, 2119
BsmI	GAATGC	1 cut(s)	157
BsnI	GGCC	6 cut(s)	517, 754, 1004, 1623, 1743, 1932
Bsp1286I	GDGCHC	4 cut(s)	53, 155, 1377, 1779
Bsp13I	TCCGGA	1 cut(s)	1155
Bsp143I	GATC	7 cut(s)	487, 721, 793, 844, 901, 988, 1788
Bsp19I	CCATGG	1 cut(s)	1540
BspANI	GGCC	6 cut(s)	517, 754, 1004, 1623, 1743, 1932
BspCNI	CTCAG	4 cut(s)	184, 1197, 1772, 1952
BspEI	TCCGGA	1 cut(s)	1155
BspFNI	CGCG	1 cut(s)	1893
BspHI	TCATGA	1 cut(s)	1414
BspLI	GGNNCC	4 cut(s)	413, 990, 1790, 1897
BspMAI	CTGCAG	3 cut(s)	1018, 1270, 1841
BspMI	ACCTGC	1 cut(s)	1538
BspPI	GGATC	7 cut(s)	482, 716, 801, 983, 996, 1783, 1796
BspQI	GCTCTTC	2 cut(s)	373, 1356
BspTI	CTTAAG	1 cut(s)	335
BsrDI	GCAATG	2 cut(s)	1675, 1727
BsrFI	RCCGGY	1 cut(s)	134
BsrI	ACTGG	3 cut(s)	464, 2053, 2099
BssAI	RCCGGY	1 cut(s)	134
BssECI	CCNNGG	4 cut(s)	959, 1099, 1540, 1738
BssMI	GATC	7 cut(s)	487, 721, 793, 844, 901, 988, 1788
BssNAI	GTATAC	1 cut(s)	1221
BssT1I	CCWWGG	2 cut(s)	1099, 1540
Bst1107I	GTATAC	1 cut(s)	1221
Bst2UI	CCWGG	5 cut(s)	891, 961, 1124, 1338, 1740
Bst6I	CTCTTC	5 cut(s)	88, 373, 762, 1356, 1640
BstAFI	CTTAAG	1 cut(s)	335
BstAPI	GCANNNNNTGC	1 cut(s)	1372
BstC8I	GCNNGC	6 cut(s)	155, 332, 519, 1047, 1373, 2013
BstDEI	CTNAG	5 cut(s)	192, 1205, 1759, 1780, 1939
BstDSI	CCRYGG	1 cut(s)	1540
BstF5I	GGATG	6 cut(s)	366, 742, 899, 1124, 1342, 2023
BstFNI	CGCG	1 cut(s)	1893
BstHHI	GCGC	3 cut(s)	316, 1539, 1684
BstKTI	GATC	7 cut(s)	490, 724, 796, 847, 904, 991, 1791
BstMAI	GTCTC	3 cut(s)	1097, 1283, 1629
BstMBI	GATC	7 cut(s)	487, 721, 793, 844, 901, 988, 1788
BstNI	CCWGG	5 cut(s)	891, 961, 1124, 1338, 1740
BstSCI	CCNGG	7 cut(s)	271, 745, 889, 959, 1122, 1336, 1738
BstSFI	CTRYAG	4 cut(s)	820, 1014, 1266, 1837
BstUI	CGCG	1 cut(s)	1893
BstV1I	GCAGC	6 cut(s)	24, 575, 1000, 1252, 1873, 1974
BstV2I	GAAGAC	2 cut(s)	1071, 2121
BstX2I	RGATCY	4 cut(s)	793, 844, 988, 1788
BstXI	CCANNNNNNTGG	1 cut(s)	1168
BstYI	RGATCY	4 cut(s)	793, 844, 988, 1788
BstZ17I	GTATAC	1 cut(s)	1221
BsuRI	GGCC	6 cut(s)	517, 754, 1004, 1623, 1743, 1932
BtgI	CCRYGG	1 cut(s)	1540
BtrI	CACGTC	1 cut(s)	604
BtsCI	GGATG	6 cut(s)	366, 742, 899, 1124, 1342, 2023
BtsI	GCAGTG	1 cut(s)	1861
BtsIMutI	CAGTG	5 cut(s)	533, 1214, 1725, 1861, 1965
BveI	ACCTGC	1 cut(s)	1538
Cac8I	GCNNGC	6 cut(s)	155, 332, 519, 1047, 1373, 2013
CaiI	CAGNNNCTG	1 cut(s)	554
CciI	TCATGA	1 cut(s)	1414
CfoI	GCGC	3 cut(s)	316, 1539, 1684
Cfr10I	RCCGGY	1 cut(s)	134
Cfr13I	GGNCC	5 cut(s)	1158, 1550, 1714, 1930, 2041
CseI	GACGC	1 cut(s)	1144
Csp6I	GTAC	4 cut(s)	401, 914, 956, 1110
CspAI	ACCGGT	1 cut(s)	134
CviQI	GTAC	4 cut(s)	401, 914, 956, 1110
DdeI	CTNAG	5 cut(s)	192, 1205, 1759, 1780, 1939
DpnI	GATC	7 cut(s)	489, 723, 795, 846, 903, 990, 1790
DpnII	GATC	7 cut(s)	487, 721, 793, 844, 901, 988, 1788
DraI	TTTAAA	1 cut(s)	1255
EaeI	YGGCCR	1 cut(s)	1002
Eam1104I	CTCTTC	5 cut(s)	88, 373, 762, 1356, 1640
EarI	CTCTTC	5 cut(s)	88, 373, 762, 1356, 1640
EciI	GGCGGA	1 cut(s)	876
Ecl136II	GAGCTC	2 cut(s)	51, 1777
Eco130I	CCWWGG	2 cut(s)	1099, 1540
Eco147I	AGGCCT	1 cut(s)	1743
Eco24I	GRGCYC	2 cut(s)	53, 1779
Eco47I	GGWCC	4 cut(s)	1158, 1550, 1714, 2041
Eco53kI	GAGCTC	2 cut(s)	51, 1777
Eco57I	CTGAAG	1 cut(s)	1602
EcoICRI	GAGCTC	2 cut(s)	51, 1777
EcoO109I	RGGNCCY	2 cut(s)	1550, 2041
EcoRII	CCWGG	5 cut(s)	889, 959, 1122, 1336, 1738
EcoT14I	CCWWGG	2 cut(s)	1099, 1540
EcoT38I	GRGCYC	2 cut(s)	53, 1779
ErhI	CCWWGG	2 cut(s)	1099, 1540
Esp3I	CGTCTC	1 cut(s)	1629
FalI	AAGNNNNNCTT	4 cut(s)	318, 350, 692, 724
FaqI	GGGAC	4 cut(s)	841, 864, 2111, 2119
FauI	CCCGC	2 cut(s)	8, 481
FblI	GTMKAC	2 cut(s)	57, 1220
Fnu4HI	GCNGC	7 cut(s)	13, 564, 1014, 1266, 1506, 1862, 1963
FokI	GGATG	6 cut(s)	353, 729, 906, 1131, 1329, 2010
FriOI	GRGCYC	2 cut(s)	53, 1779
Fsp4HI	GCNGC	7 cut(s)	13, 564, 1014, 1266, 1506, 1862, 1963
FspBI	CTAG	4 cut(s)	996, 1563, 1901, 2076
GlaI	GCGC	3 cut(s)	315, 1538, 1683
GluI	GCNGC	7 cut(s)	13, 564, 1014, 1266, 1506, 1862, 1963
GsuI	CTGGAG	2 cut(s)	477, 2057
HaeIII	GGCC	6 cut(s)	517, 754, 1004, 1623, 1743, 1932
HapII	CCGG	4 cut(s)	135, 272, 747, 1156
HgaI	GACGC	1 cut(s)	1144
HhaI	GCGC	3 cut(s)	316, 1539, 1684
Hin6I	GCGC	3 cut(s)	314, 1537, 1682
HinP1I	GCGC	3 cut(s)	314, 1537, 1682
HincII	GTYRAC	2 cut(s)	58, 1174
HindII	GTYRAC	2 cut(s)	58, 1174
HpaII	CCGG	4 cut(s)	135, 272, 747, 1156
HphI	GGTGA	4 cut(s)	124, 878, 1259, 1790
Hpy166II	GTNNAC	7 cut(s)	58, 808, 1112, 1174, 1221, 1388, 1834
Hpy188III	TCNNGA	8 cut(s)	370, 626, 786, 1156, 1282, 1358, 1415, 2036
Hpy8I	GTNNAC	7 cut(s)	58, 808, 1112, 1174, 1221, 1388, 1834
Hpy99I	CGWCG	4 cut(s)	59, 62, 116, 2105
HpyAV	CCTTC	5 cut(s)	213, 296, 355, 1065, 1814
HpyCH4IV	ACGT	2 cut(s)	603, 1166
HpyF3I	CTNAG	5 cut(s)	192, 1205, 1759, 1780, 1939
HpySE526I	ACGT	2 cut(s)	603, 1166
HspAI	GCGC	3 cut(s)	314, 1537, 1682
Kpn2I	TCCGGA	1 cut(s)	1155
Kzo9I	GATC	7 cut(s)	487, 721, 793, 844, 901, 988, 1788
LguI	GCTCTTC	2 cut(s)	373, 1356
LmnI	GCTCC	7 cut(s)	4, 56, 269, 447, 1103, 1544, 1753
Lsp1109I	GCAGC	6 cut(s)	24, 575, 1000, 1252, 1873, 1974
LweI	GCATC	5 cut(s)	117, 251, 664, 1109, 1804
MaeI	CTAG	4 cut(s)	996, 1563, 1901, 2076
MaeII	ACGT	2 cut(s)	603, 1166
MaeIII	GTNAC	7 cut(s)	229, 581, 695, 702, 1258, 1658, 1729
MalI	GATC	7 cut(s)	489, 723, 795, 846, 903, 990, 1790
MboI	GATC	7 cut(s)	487, 721, 793, 844, 901, 988, 1788
MboII	GAAGA	8 cut(s)	75, 390, 604, 749, 1071, 1373, 1657, 2126
MflI	RGATCY	4 cut(s)	793, 844, 988, 1788
MhlI	GDGCHC	4 cut(s)	53, 155, 1377, 1779
MlsI	TGGCCA	1 cut(s)	1004
MluCI	AATT	9 cut(s)	32, 454, 508, 634, 1024, 1250, 1404, 2000, 2081
MluNI	TGGCCA	1 cut(s)	1004
MlyI	GAGTC	6 cut(s)	72, 110, 203, 838, 1363, 1565
MmeI	TCCRAC	2 cut(s)	1016, 2070
Mox20I	TGGCCA	1 cut(s)	1004
MroI	TCCGGA	1 cut(s)	1155
MroXI	GAANNNNTTC	1 cut(s)	306
MscI	TGGCCA	1 cut(s)	1004
MslI	CAYNNNNRTG	4 cut(s)	909, 1117, 1803, 1970
Msp20I	TGGCCA	1 cut(s)	1004
MspA1I	CMGCKG	4 cut(s)	15, 1204, 1265, 1298
MspCI	CTTAAG	1 cut(s)	335
MspI	CCGG	4 cut(s)	135, 272, 747, 1156
MspR9I	CCNGG	7 cut(s)	273, 747, 891, 961, 1124, 1338, 1740
Mva1269I	GAATGC	1 cut(s)	157
MvaI	CCWGG	5 cut(s)	891, 961, 1124, 1338, 1740
MvnI	CGCG	1 cut(s)	1893
NciI	CCSGG	2 cut(s)	273, 747
NcoI	CCATGG	1 cut(s)	1540
NdeII	GATC	7 cut(s)	487, 721, 793, 844, 901, 988, 1788
NlaIV	GGNNCC	4 cut(s)	413, 990, 1790, 1897
NmuCI	GTSAC	2 cut(s)	695, 1258
OliI	CACNNNNGTG	1 cut(s)	1970
PagI	TCATGA	1 cut(s)	1414
PceI	AGGCCT	1 cut(s)	1743
PciSI	GCTCTTC	2 cut(s)	373, 1356
PcsI	WCGNNNNNNNCGW	1 cut(s)	111
PctI	GAATGC	1 cut(s)	157
PdmI	GAANNNNTTC	1 cut(s)	306
PfeI	GAWTC	7 cut(s)	209, 221, 276, 551, 629, 1691, 1888
PfoI	TCCNGGA	1 cut(s)	271
PinAI	ACCGGT	1 cut(s)	134
PkrI	GCNGC	7 cut(s)	14, 565, 1015, 1267, 1507, 1863, 1964
PleI	GAGTC	6 cut(s)	72, 110, 202, 837, 1362, 1565
PpsI	GAGTC	6 cut(s)	72, 110, 202, 837, 1362, 1565
PpuMI	RGGWCCY	2 cut(s)	1550, 2041
Psp124BI	GAGCTC	2 cut(s)	53, 1779
Psp5II	RGGWCCY	2 cut(s)	1550, 2041
Psp6I	CCWGG	5 cut(s)	889, 959, 1122, 1336, 1738
PspGI	CCWGG	5 cut(s)	889, 959, 1122, 1336, 1738
PspN4I	GGNNCC	4 cut(s)	413, 990, 1790, 1897
PspPI	GGNCC	5 cut(s)	1158, 1550, 1714, 1930, 2041
PspPPI	RGGWCCY	2 cut(s)	1550, 2041
PstI	CTGCAG	3 cut(s)	1018, 1270, 1841
PstNI	CAGNNNCTG	1 cut(s)	554
PsuI	RGATCY	4 cut(s)	793, 844, 988, 1788
PvuII	CAGCTG	2 cut(s)	1265, 1298
RsaI	GTAC	4 cut(s)	402, 915, 957, 1111
RsaNI	GTAC	4 cut(s)	401, 914, 956, 1110
RseI	CAYNNNNRTG	4 cut(s)	909, 1117, 1803, 1970
SacI	GAGCTC	2 cut(s)	53, 1779
SalI	GTCGAC	1 cut(s)	56
SapI	GCTCTTC	2 cut(s)	373, 1356
SatI	GCNGC	7 cut(s)	13, 564, 1014, 1266, 1506, 1862, 1963
Sau3AI	GATC	7 cut(s)	487, 721, 793, 844, 901, 988, 1788
Sau96I	GGNCC	5 cut(s)	1158, 1550, 1714, 1930, 2041
ScaI	AGTACT	1 cut(s)	402
SchI	GAGTC	6 cut(s)	72, 110, 203, 838, 1363, 1565
ScrFI	CCNGG	7 cut(s)	273, 747, 891, 961, 1124, 1338, 1740
SduI	GDGCHC	4 cut(s)	53, 155, 1377, 1779
SfaNI	GCATC	5 cut(s)	117, 251, 664, 1109, 1804
SfcI	CTRYAG	4 cut(s)	820, 1014, 1266, 1837
SgrDI	CGTCGACG	1 cut(s)	56
SinI	GGWCC	4 cut(s)	1158, 1550, 1714, 2041
SmiMI	CAYNNNNRTG	4 cut(s)	909, 1117, 1803, 1970
SmlI	CTYRAG	6 cut(s)	162, 335, 586, 784, 1085, 1280
SmoI	CTYRAG	6 cut(s)	162, 335, 586, 784, 1085, 1280
Sse9I	AATT	9 cut(s)	32, 454, 508, 634, 1024, 1250, 1404, 2000, 2081
SseBI	AGGCCT	1 cut(s)	1743
SspI	AATATT	1 cut(s)	1290
SspMI	CTAG	4 cut(s)	996, 1563, 1901, 2076
SstI	GAGCTC	2 cut(s)	53, 1779
StuI	AGGCCT	1 cut(s)	1743
StyD4I	CCNGG	7 cut(s)	271, 745, 889, 959, 1122, 1336, 1738
StyI	CCWWGG	2 cut(s)	1099, 1540
TaiI	ACGT	2 cut(s)	606, 1169
TaqI	TCGA	5 cut(s)	57, 111, 212, 1377, 2085
TasI	AATT	9 cut(s)	32, 454, 508, 634, 1024, 1250, 1404, 2000, 2081
TatI	WGTACW	1 cut(s)	400
TauI	GCSGC	1 cut(s)	1508
TfiI	GAWTC	7 cut(s)	209, 221, 276, 551, 629, 1691, 1888
TscAI	CASTG	5 cut(s)	533, 1221, 1732, 1861, 1972
TseFI	GTSAC	2 cut(s)	695, 1258
TseI	GCWGC	6 cut(s)	12, 563, 1013, 1265, 1861, 1962
Tsp45I	GTSAC	2 cut(s)	695, 1258
TspDTI	ATGAA	4 cut(s)	299, 611, 2081, 2127
TspGWI	ACGGA	2 cut(s)	43, 256
TspRI	CASTG	5 cut(s)	533, 1221, 1732, 1861, 1972
Vha464I	CTTAAG	1 cut(s)	335
VpaK11BI	GGWCC	4 cut(s)	1158, 1550, 1714, 2041
XapI	RAATTY	6 cut(s)	508, 634, 1024, 1250, 2000, 2081
XmiI	GTMKAC	2 cut(s)	57, 1220
XmnI	GAANNNNTTC	1 cut(s)	306
XspI	CTAG	4 cut(s)	996, 1563, 1901, 2076
ZrmI	AGTACT	1 cut(s)	402

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.