Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig01014

Orthogroup ID

OG0012217

Physical Location & Seq

Genomic Coordinates Reverse (-)

8453 .. 12781

Gene Structure 4329 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G021850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGGAGGCGAAGAATAGCGGCAAACCCATACTTCAATTCGCACCGTTTCAGAGCTCCGTCGACGAGGGCTTCTGGCACAGATTGTCTTCTTTGAAGCTCAACAAGTTTGGCATCGACGACTCTCCAATTCCCATCACTGGGTTCTATGCGCCGTGCTCACATTCTCAGGTATCAAATCATTTGACTCTTCTAGCTGAGTCTTTGCCTTCGGATTCGAATGAAGAATCGAGTGAACCAGGAGTGATTCGGGGAAATAGGAATAGATGCTCCGTTCCCGGTATTCTTTACAACACCAATACGGTAGAAGGCTTTCGTGCACTTGACAAACAGGAGTTGATTAAAGCAGAAGCGAAAAAGATTTGGGAGGACATTCAGAATGGAAAAGCTTTGGAGGATAGTTCTGTACTTTCCAGGTTCCTGCTTATCTCTTTTGCAGACCTTAAAAAATGGAGCTTTCATTACTGGTTTGCCTTCCCTGCTCTGGTGCTTGATCCTCCAGCAACATTGCTTGATTTGAAACCAGCTTCACAATTTTTTAGCGTGAAGGAGGCAGAATCTGTCTCAGCAGTATTAAATGACTGGCGCGACTCAAGCTTAGCTACAGATGTGCCGTTCTTTTTGGTTGTAATTGATCCAAATTCACATGCTACTATTAAGCATCTAAAGGATTGGGAAAGTTGTCAAAGAGCTACTGATCACAAGTTGCTATTTGGTTTTTACGACCCATGCCATCTTCCAAAGAATCCTGGTTGGCCGCTTCGGAACTTGCTTGCACTTATTTGCTCAAGATGGGATCTCAAGTCTGTTCGCTTTCTATGCTATAGAGAGACTCGTGGTTTCGCAGATCTAGGATTGTCCCTTGTTGGTGAAGCCCAGGTTACAGTTCCCCAAGGATGGCGAGATCTTCATAATACGCCCAATGCAGTGGGGTGGGAACTTAACAAAGGGAAAAAAATACCCAGGTGTATTAGCCTTGCTAAATCTATGGATCCAACTAGGTTGGCCATATCTGCTGCAGATTTGAATTTGAAACTAATGAGATGGCGTGCTTTACCTTCTCTCAAGTTAAATACCTTGTCCTCTCTCAAGTGTCTCCTCCTTGGAGCAGGTACACTTGGATGCCAGGTTGCTCGCATGCTTATGGCTTGGGGCGTTCAGAAAATTACGTTGGTTGACAATGGCAGAGTGGCTATGTCTAATCCCTTGAGGCAATCCCTGTATACATTAGATGACTGTCTAAATGGTGGTGAATTTAAAGCTGTAGCAGCAGTTAACAGTCTCAAGAAAATATTTCCCGCTGTGGAAGCAGAGGGTGTTGTCATGGCTATACCAATGCCTGGACATCCCGTTCCTAGTCAAGAAGAGCAGAGTGTGCTTGATGATTGTAGGCGCCTACATGATTTAATTGATTCTCATGATGCAGTATTCTTGTTAACTGATACAAGAGAAAGTCGGTGGCTTCCGTCTCTTCTTTGTGCCAATACTAACAAGATTACTATAACTGCAGCTCTTGGGTTTGACAGCTTTTTAGTTATGCGCCATGGAGCAGGTCCATCCAGCTCTAGCCATGACTCAAAATCTGAAGCTGCAAATGCTTTATCCACTGATCTGAACAGCCTTGCACTGACTGATAGAAATGGAGGGCACAGATTGGGTTGCTACTTCTGCAATGATGTGGTTGCACCTATTGATTCAACTGCCAACCGCACCTTGGACCAGCAATGCACTGTTACACGCCCAGGCCTCGCTCCTATTGCCTCAGCTCTTGCAGTTGAGCTCTTAGTTGGGATCCTACATCATCCTGATGGGATATTTGCCGAAGGTGAAATTGTAAACTCTAGTAATAATGGAAGCAGTGAGCAACCTCTTGGTATTTTACCCCATCAAATCCGTGGTTCCCTTGGACAGTTTTCGCAGATGACACTTGTGGGCCACTCCTCAGACAGTTGCACGGCTTGCTGCAGCATTGTGGTGTCTGAATATCGAAAAAGGGGAATGGAATTTATACTACAAGCAATCAACCATCCCACCTATCTGGAGGACGTGACTGGACTAACAGAGCTGATGAAAACAGCTCATGCATTTCAAATGGATTGGGATGATGGGACTGATAATGATGATGATGATTGTGTTGAAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000407 GO:0000422 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006497 GO:0006501 GO:0006807 GO:0006914 GO:0006950 GO:0006952 GO:0006995 GO:0006996 GO:0007005 GO:0007033 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008641 GO:0009056 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0009991 GO:0010150 GO:0016043 GO:0016236 GO:0016237 GO:0016740 GO:0016874 GO:0016877 GO:0018410 GO:0019538 GO:0019778 GO:0019779 GO:0022411 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0034645 GO:0034727 GO:0036211 GO:0042157 GO:0042158 GO:0042594 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043687 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044804 GO:0044805 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061726 GO:0061919 GO:0070925 GO:0071496 GO:0071704 GO:0071840 GO:0090693 GO:0098542 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1903008 GO:1905037

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 ko04216 map04136 map04138 map04140 map04216

Pfam Domains

Protein Families

ATG7_N ThiF

Protein Analysis

713

Amino Acids

78.38

Weight (kDa)

5.73

Isoelectric Point (pI)

40.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG7_N	PF16420	11 - 329	2.7e-103	Ubiquitin-like modifier-activating enzyme ATG7 N-terminus
ThiF	PF00899	346 - 602	8.7e-38	ThiF family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45900
fragaria_vesca	FvH4_3g07910
malus_domestica	MD05G1302300.v1.1 MD10G1281000.v1.1
prunus_persica	Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1
pyrus_communis	pycom05g27990 pycom10g23460
rosa_chinensis	RchiOBHm_Chr5g0010641
rosa_laevigata	RLG00000031768
rosa_multiflora	Rmu_sc0006103.1_g000002 Rmu_sc0014635.1_g000003
rosa_roxburghii	Rroxscaffold_1G00065730
rosa_rugosa	Rorug04G0451400.1
rosa_samantha	Rh5AG083000 Rh5CG091000
rosa_wichuraiana	Rw0G000680 Rw0G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1097, 1538
AccB1I	GGYRCC	1 cut(s)	1389
AccB7I	CCANNNNNTGG	1 cut(s)	1337
AccI	GTMKAC	2 cut(s)	60, 1220
AccII	CGCG	1 cut(s)	585
AciI	CCGC	4 cut(s)	18, 755, 1296, 1705
AclWI	GGATC	7 cut(s)	485, 626, 801, 983, 996, 1783, 1796
AcoI	YGGCCR	2 cut(s)	752, 1002
AcsI	RAATTY	5 cut(s)	637, 1024, 1250, 2000, 2136
AcuI	CTGAAG	1 cut(s)	1602
AcyI	GRCGYC	1 cut(s)	1390
AfaI	GTAC	2 cut(s)	405, 1111
AfiI	CCNNNNNNNGG	6 cut(s)	137, 138, 481, 894, 1337, 1711
AgsI	TTSAA	8 cut(s)	35, 94, 517, 1024, 1030, 1695, 2087, 2135
AjiI	CACGTC	1 cut(s)	2044
AjnI	CCWGG	8 cut(s)	235, 410, 745, 873, 959, 1122, 1336, 1738
AjuI	GAANNNNNNNTTGG	2 cut(s)	1151, 1183
AloI	GAACNNNNNNTCC	2 cut(s)	1332, 1364
Alw21I	GWGCWC	4 cut(s)	56, 158, 319, 1779
Alw26I	GTCTC	5 cut(s)	565, 821, 1097, 1283, 1470
Alw44I	GTGCAC	1 cut(s)	315
AlwI	GGATC	7 cut(s)	485, 626, 801, 983, 996, 1783, 1796
AlwNI	CAGNNNCTG	1 cut(s)	557
AoxI	GGCC	4 cut(s)	752, 1002, 1741, 1930
ApaLI	GTGCAC	1 cut(s)	315
ApeKI	GCWGC	6 cut(s)	1013, 1265, 1505, 1586, 1959, 1962
ApoI	RAATTY	5 cut(s)	637, 1024, 1250, 2000, 2136
Asp700I	GAANNNNTTC	1 cut(s)	309
AspLEI	GCGC	4 cut(s)	151, 585, 1392, 1539
AspS9I	GGNCC	3 cut(s)	1550, 1714, 1930
AsuC2I	CCSGG	1 cut(s)	276
AsuHPI	GGTGA	3 cut(s)	878, 1259, 1835
AsuII	TTCGAA	1 cut(s)	215
AvaII	GGWCC	2 cut(s)	1550, 1714
BaeGI	GKGCMC	2 cut(s)	319, 1647
BalI	TGGCCA	1 cut(s)	1004
BamHI	GGATCC	2 cut(s)	988, 1788
BanI	GGYRCC	1 cut(s)	1389
BanII	GRGCYC	2 cut(s)	56, 1779
BauI	CACGAG	1 cut(s)	831
BbsI	GAAGAC	1 cut(s)	78
Bbv12I	GWGCWC	4 cut(s)	56, 158, 319, 1779
BbvCI	CCTCAGC	1 cut(s)	1759
BbvI	GCAGC	6 cut(s)	1000, 1277, 1517, 1573, 1946, 1974
BceAI	ACGGC	3 cut(s)	136, 595, 1968
BciT130I	CCWGG	8 cut(s)	237, 412, 747, 875, 961, 1124, 1338, 1740
BclI	TGATCA	1 cut(s)	694
BcnI	CCSGG	1 cut(s)	276
BcoDI	GTCTC	5 cut(s)	565, 821, 1097, 1283, 1470
BfaI	CTAG	6 cut(s)	191, 848, 996, 1353, 1563, 1839
BfmI	CTRYAG	6 cut(s)	600, 820, 1014, 1260, 1503, 1960
BfoI	RGCGCY	1 cut(s)	1393
BfuAI	ACCTGC	2 cut(s)	1097, 1538
BglII	AGATCT	2 cut(s)	844, 901
BisI	GCNGC	8 cut(s)	19, 755, 1014, 1266, 1506, 1587, 1960, 1963
BlpI	GCTNAGC	1 cut(s)	595
BlsI	GCNGC	8 cut(s)	20, 756, 1015, 1267, 1507, 1588, 1961, 1964
Bme1390I	CCNGG	9 cut(s)	237, 276, 412, 747, 875, 961, 1124, 1338, 1740
Bme18I	GGWCC	2 cut(s)	1550, 1714
BmgBI	CACGTC	1 cut(s)	2044
BmgT120I	GGNCC	3 cut(s)	1550, 1714, 1930
BmiI	GGNNCC	5 cut(s)	416, 990, 1391, 1790, 1897
BmrFI	CCNGG	9 cut(s)	237, 276, 412, 747, 875, 961, 1124, 1338, 1740
BmrI	ACTGGG	1 cut(s)	147
BmsI	GCATC	5 cut(s)	120, 254, 667, 1109, 1408
BmuI	ACTGGG	1 cut(s)	147
BpiI	GAAGAC	1 cut(s)	78
BpmI	CTGGAG	2 cut(s)	480, 2057
Bpu10I	CCTNAGC	1 cut(s)	1759
Bpu1102I	GCTNAGC	1 cut(s)	595
Bpu14I	TTCGAA	1 cut(s)	215
BpuEI	CTTGAG	7 cut(s)	574, 769, 782, 1046, 1070, 1225, 1265
BpuMI	CCSGG	1 cut(s)	276
BsaHI	GRCGYC	1 cut(s)	1390
BsaJI	CCNNGG	9 cut(s)	873, 889, 959, 1099, 1540, 1710, 1738, 1891, 1900
Bsc4I	CCNNNNNNNGG	6 cut(s)	137, 138, 481, 894, 1337, 1711
Bse1I	ACTGG	4 cut(s)	142, 467, 584, 2053
Bse3DI	GCAATG	3 cut(s)	503, 1675, 1727
BseBI	CCWGG	8 cut(s)	237, 412, 747, 875, 961, 1124, 1338, 1740
BseDI	CCNNGG	9 cut(s)	873, 889, 959, 1099, 1540, 1710, 1738, 1891, 1900
BseGI	GGATG	7 cut(s)	899, 1124, 1342, 1553, 1798, 2023, 2104
BseLI	CCNNNNNNNGG	6 cut(s)	137, 138, 481, 894, 1337, 1711
BseMI	GCAATG	3 cut(s)	503, 1675, 1727
BseMII	CTCAG	5 cut(s)	179, 186, 576, 1773, 1953
BseNI	ACTGG	4 cut(s)	142, 467, 584, 2053
BseRI	GAGGAG	2 cut(s)	1085, 1927
BseSI	GKGCMC	2 cut(s)	319, 1647
BseXI	GCAGC	6 cut(s)	1000, 1277, 1517, 1573, 1946, 1974
Bsh1236I	CGCG	1 cut(s)	585
BshFI	GGCC	4 cut(s)	754, 1004, 1743, 1932
BshNI	GGYRCC	1 cut(s)	1389
BsiHKAI	GWGCWC	4 cut(s)	56, 158, 319, 1779
BsiSI	CCGG	1 cut(s)	276
BslFI	GGGAC	2 cut(s)	841, 2119
BslI	CCNNNNNNNGG	6 cut(s)	137, 138, 481, 894, 1337, 1711
BsmAI	GTCTC	5 cut(s)	565, 821, 1097, 1283, 1470
BsmBI	CGTCTC	1 cut(s)	1470
BsmFI	GGGAC	2 cut(s)	841, 2119
BsnI	GGCC	4 cut(s)	754, 1004, 1743, 1932
Bsp119I	TTCGAA	1 cut(s)	215
Bsp1286I	GDGCHC	5 cut(s)	56, 158, 319, 1647, 1779
Bsp143I	GATC	9 cut(s)	490, 631, 694, 793, 844, 901, 988, 1606, 1788
Bsp1720I	GCTNAGC	1 cut(s)	595
Bsp19I	CCATGG	1 cut(s)	1540
BspACI	CCGC	4 cut(s)	18, 755, 1296, 1705
BspANI	GGCC	4 cut(s)	754, 1004, 1743, 1932
BspCNI	CTCAG	5 cut(s)	178, 187, 575, 1772, 1952
BspFNI	CGCG	1 cut(s)	585
BspHI	TCATGA	1 cut(s)	1414
BspLI	GGNNCC	5 cut(s)	416, 990, 1391, 1790, 1897
BspMAI	CTGCAG	3 cut(s)	1018, 1507, 1964
BspMI	ACCTGC	2 cut(s)	1097, 1538
BspPI	GGATC	7 cut(s)	485, 626, 801, 983, 996, 1783, 1796
BspQI	GCTCTTC	1 cut(s)	1356
BspT104I	TTCGAA	1 cut(s)	215
BspT107I	GGYRCC	1 cut(s)	1389
BsrDI	GCAATG	3 cut(s)	503, 1675, 1727
BsrI	ACTGG	4 cut(s)	142, 467, 584, 2053
BssECI	CCNNGG	9 cut(s)	873, 889, 959, 1099, 1540, 1710, 1738, 1891, 1900
BssMI	GATC	9 cut(s)	490, 631, 694, 793, 844, 901, 988, 1606, 1788
BssNAI	GTATAC	1 cut(s)	1221
BssNI	GRCGYC	1 cut(s)	1390
BssSI	CACGAG	1 cut(s)	831
BssT1I	CCWWGG	5 cut(s)	889, 1099, 1540, 1710, 1900
Bst1107I	GTATAC	1 cut(s)	1221
Bst2BI	CACGAG	1 cut(s)	831
Bst2UI	CCWGG	8 cut(s)	237, 412, 747, 875, 961, 1124, 1338, 1740
Bst4CI	ACNGT	8 cut(s)	45, 301, 883, 1235, 1277, 1729, 1908, 1946
Bst6I	CTCTTC	3 cut(s)	192, 1356, 1473
BstACI	GRCGYC	1 cut(s)	1390
BstAPI	GCANNNNNTGC	2 cut(s)	1372, 1956
BstBI	TTCGAA	1 cut(s)	215
BstC8I	GCNNGC	5 cut(s)	771, 1047, 1132, 1136, 1957
BstDEI	CTNAG	7 cut(s)	165, 195, 562, 595, 1759, 1780, 1939
BstDSI	CCRYGG	2 cut(s)	1540, 1891
BstF5I	GGATG	7 cut(s)	899, 1124, 1342, 1553, 1798, 2023, 2104
BstFNI	CGCG	1 cut(s)	585
BstH2I	RGCGCY	1 cut(s)	1393
BstHHI	GCGC	4 cut(s)	151, 585, 1392, 1539
BstKTI	GATC	9 cut(s)	493, 634, 697, 796, 847, 904, 991, 1609, 1791
BstMAI	GTCTC	5 cut(s)	565, 821, 1097, 1283, 1470
BstMBI	GATC	9 cut(s)	490, 631, 694, 793, 844, 901, 988, 1606, 1788
BstNI	CCWGG	8 cut(s)	237, 412, 747, 875, 961, 1124, 1338, 1740
BstNSI	RCATGY	2 cut(s)	647, 1138
BstSCI	CCNGG	9 cut(s)	235, 274, 410, 745, 873, 959, 1122, 1336, 1738
BstSFI	CTRYAG	6 cut(s)	600, 820, 1014, 1260, 1503, 1960
BstSLI	GKGCMC	2 cut(s)	319, 1647
BstUI	CGCG	1 cut(s)	585
BstV1I	GCAGC	6 cut(s)	1000, 1277, 1517, 1573, 1946, 1974
BstV2I	GAAGAC	1 cut(s)	78
BstX2I	RGATCY	5 cut(s)	793, 844, 901, 988, 1788
BstXI	CCANNNNNNTGG	2 cut(s)	925, 2035
BstYI	RGATCY	5 cut(s)	793, 844, 901, 988, 1788
BstZ17I	GTATAC	1 cut(s)	1221
BsuRI	GGCC	4 cut(s)	754, 1004, 1743, 1932
BtgI	CCRYGG	2 cut(s)	1540, 1891
BtrI	CACGTC	1 cut(s)	2044
BtsCI	GGATG	7 cut(s)	899, 1124, 1342, 1553, 1798, 2023, 2104
BtsI	GCAGTG	2 cut(s)	930, 1861
BtsIMutI	CAGTG	6 cut(s)	135, 930, 1602, 1622, 1725, 1861
BveI	ACCTGC	2 cut(s)	1097, 1538
Cac8I	GCNNGC	5 cut(s)	771, 1047, 1132, 1136, 1957
CaiI	CAGNNNCTG	1 cut(s)	557
CciI	TCATGA	1 cut(s)	1414
CfoI	GCGC	4 cut(s)	151, 585, 1392, 1539
Cfr13I	GGNCC	3 cut(s)	1550, 1714, 1930
Csp6I	GTAC	2 cut(s)	404, 1110
CviAII	CATG	9 cut(s)	644, 726, 1135, 1321, 1397, 1415, 1541, 1568, 2078
CviQI	GTAC	2 cut(s)	404, 1110
DdeI	CTNAG	7 cut(s)	165, 195, 562, 595, 1759, 1780, 1939
DinI	GGCGCC	1 cut(s)	1391
DpnI	GATC	9 cut(s)	492, 633, 696, 795, 846, 903, 990, 1608, 1790
DpnII	GATC	9 cut(s)	490, 631, 694, 793, 844, 901, 988, 1606, 1788
DraI	TTTAAA	1 cut(s)	1255
EaeI	YGGCCR	2 cut(s)	752, 1002
Eam1104I	CTCTTC	3 cut(s)	192, 1356, 1473
EarI	CTCTTC	3 cut(s)	192, 1356, 1473
Ecl136II	GAGCTC	2 cut(s)	54, 1777
Eco130I	CCWWGG	5 cut(s)	889, 1099, 1540, 1710, 1900
Eco147I	AGGCCT	1 cut(s)	1743
Eco24I	GRGCYC	2 cut(s)	56, 1779
Eco47I	GGWCC	2 cut(s)	1550, 1714
Eco53kI	GAGCTC	2 cut(s)	54, 1777
Eco57I	CTGAAG	1 cut(s)	1602
EcoICRI	GAGCTC	2 cut(s)	54, 1777
EcoRII	CCWGG	8 cut(s)	235, 410, 745, 873, 959, 1122, 1336, 1738
EcoT14I	CCWWGG	5 cut(s)	889, 1099, 1540, 1710, 1900
EcoT22I	ATGCAT	1 cut(s)	2083
EcoT38I	GRGCYC	2 cut(s)	56, 1779
EgeI	GGCGCC	1 cut(s)	1391
EheI	GGCGCC	1 cut(s)	1391
ErhI	CCWWGG	5 cut(s)	889, 1099, 1540, 1710, 1900
Esp3I	CGTCTC	1 cut(s)	1470
FaeI	CATG	9 cut(s)	647, 729, 1138, 1324, 1400, 1418, 1544, 1571, 2081
FaqI	GGGAC	2 cut(s)	841, 2119
FatI	CATG	9 cut(s)	643, 725, 1134, 1320, 1396, 1414, 1540, 1567, 2077
FauI	CCCGC	1 cut(s)	1303
FbaI	TGATCA	1 cut(s)	694
FblI	GTMKAC	2 cut(s)	60, 1220
Fnu4HI	GCNGC	8 cut(s)	19, 755, 1014, 1266, 1506, 1587, 1960, 1963
FokI	GGATG	7 cut(s)	906, 1131, 1329, 1540, 1785, 2010, 2111
FriOI	GRGCYC	2 cut(s)	56, 1779
Fsp4HI	GCNGC	8 cut(s)	19, 755, 1014, 1266, 1506, 1587, 1960, 1963
FspBI	CTAG	6 cut(s)	191, 848, 996, 1353, 1563, 1839
GlaI	GCGC	4 cut(s)	150, 584, 1391, 1538
GluI	GCNGC	8 cut(s)	19, 755, 1014, 1266, 1506, 1587, 1960, 1963
GsuI	CTGGAG	2 cut(s)	480, 2057
HaeII	RGCGCY	1 cut(s)	1393
HaeIII	GGCC	4 cut(s)	754, 1004, 1743, 1932
HapII	CCGG	1 cut(s)	276
HhaI	GCGC	4 cut(s)	151, 585, 1392, 1539
Hin1I	GRCGYC	1 cut(s)	1390
Hin1II	CATG	9 cut(s)	647, 729, 1138, 1324, 1400, 1418, 1544, 1571, 2081
Hin6I	GCGC	4 cut(s)	149, 583, 1390, 1537
HinP1I	GCGC	4 cut(s)	149, 583, 1390, 1537
HincII	GTYRAC	4 cut(s)	61, 1174, 1273, 1434
HindII	GTYRAC	4 cut(s)	61, 1174, 1273, 1434
HindIII	AAGCTT	2 cut(s)	384, 592
HpaI	GTTAAC	2 cut(s)	1273, 1434
HpaII	CCGG	1 cut(s)	276
HphI	GGTGA	3 cut(s)	878, 1259, 1835
Hpy166II	GTNNAC	9 cut(s)	61, 233, 317, 1112, 1174, 1221, 1273, 1434, 1834
Hpy188I	TCNGA	9 cut(s)	51, 211, 375, 762, 1158, 1582, 1611, 1942, 1978
Hpy188III	TCNNGA	6 cut(s)	786, 1282, 1358, 1415, 1802, 2036
Hpy8I	GTNNAC	9 cut(s)	61, 233, 317, 1112, 1174, 1221, 1273, 1434, 1834
Hpy99I	CGWCG	3 cut(s)	62, 65, 119
HpyAV	CCTTC	6 cut(s)	216, 299, 481, 538, 1065, 1814
HpyCH4III	ACNGT	8 cut(s)	45, 301, 883, 1235, 1277, 1729, 1908, 1946
HpyCH4IV	ACGT	2 cut(s)	1166, 2043
HpyF3I	CTNAG	7 cut(s)	165, 195, 562, 595, 1759, 1780, 1939
HpySE526I	ACGT	2 cut(s)	1166, 2043
Hsp92I	GRCGYC	1 cut(s)	1390
Hsp92II	CATG	9 cut(s)	647, 729, 1138, 1324, 1400, 1418, 1544, 1571, 2081
HspAI	GCGC	4 cut(s)	149, 583, 1390, 1537
KasI	GGCGCC	1 cut(s)	1389
Ksp22I	TGATCA	1 cut(s)	694
KspAI	GTTAAC	2 cut(s)	1273, 1434
Kzo9I	GATC	9 cut(s)	490, 631, 694, 793, 844, 901, 988, 1606, 1788
LguI	GCTCTTC	1 cut(s)	1356
LmnI	GCTCC	6 cut(s)	59, 272, 450, 1103, 1544, 1753
Lsp1109I	GCAGC	6 cut(s)	1000, 1277, 1517, 1573, 1946, 1974
LweI	GCATC	5 cut(s)	120, 254, 667, 1109, 1408
MaeI	CTAG	6 cut(s)	191, 848, 996, 1353, 1563, 1839
MaeII	ACGT	2 cut(s)	1166, 2043
MaeIII	GTNAC	3 cut(s)	877, 1729, 2044
MalI	GATC	9 cut(s)	492, 633, 696, 795, 846, 903, 990, 1608, 1790
MboI	GATC	9 cut(s)	490, 631, 694, 793, 844, 901, 988, 1606, 1788
MboII	GAAGA	8 cut(s)	22, 78, 179, 233, 725, 896, 1373, 1460
MflI	RGATCY	5 cut(s)	793, 844, 901, 988, 1788
MhlI	GDGCHC	5 cut(s)	56, 158, 319, 1647, 1779
MlsI	TGGCCA	1 cut(s)	1004
MluNI	TGGCCA	1 cut(s)	1004
Mly113I	GGCGCC	1 cut(s)	1390
MlyI	GAGTC	6 cut(s)	113, 178, 206, 581, 823, 1565
MmeI	TCCRAC	1 cut(s)	1016
Mox20I	TGGCCA	1 cut(s)	1004
Mph1103I	ATGCAT	1 cut(s)	2083
MroXI	GAANNNNTTC	1 cut(s)	309
MscI	TGGCCA	1 cut(s)	1004
MslI	CAYNNNNRTG	4 cut(s)	1117, 1139, 1803, 1970
Msp20I	TGGCCA	1 cut(s)	1004
MspA1I	CMGCKG	1 cut(s)	1298
MspI	CCGG	1 cut(s)	276
MspR9I	CCNGG	9 cut(s)	237, 276, 412, 747, 875, 961, 1124, 1338, 1740
MvaI	CCWGG	8 cut(s)	237, 412, 747, 875, 961, 1124, 1338, 1740
MvnI	CGCG	1 cut(s)	585
NarI	GGCGCC	1 cut(s)	1390
NciI	CCSGG	1 cut(s)	276
NcoI	CCATGG	1 cut(s)	1540
NdeII	GATC	9 cut(s)	490, 631, 694, 793, 844, 901, 988, 1606, 1788
NlaIII	CATG	9 cut(s)	647, 729, 1138, 1324, 1400, 1418, 1544, 1571, 2081
NlaIV	GGNNCC	5 cut(s)	416, 990, 1391, 1790, 1897
NmuCI	GTSAC	1 cut(s)	2044
NsiI	ATGCAT	1 cut(s)	2083
NspI	RCATGY	2 cut(s)	647, 1138
NspV	TTCGAA	1 cut(s)	215
PaeI	GCATGC	1 cut(s)	1138
PagI	TCATGA	1 cut(s)	1414
PceI	AGGCCT	1 cut(s)	1743
PciSI	GCTCTTC	1 cut(s)	1356
PdmI	GAANNNNTTC	1 cut(s)	309
PfeI	GAWTC	7 cut(s)	212, 224, 244, 554, 742, 1409, 1691
PflMI	CCANNNNNTGG	1 cut(s)	1337
PkrI	GCNGC	8 cut(s)	20, 756, 1015, 1267, 1507, 1588, 1961, 1964
PleI	GAGTC	6 cut(s)	113, 178, 205, 581, 823, 1565
PluTI	GGCGCC	1 cut(s)	1393
PpsI	GAGTC	6 cut(s)	113, 178, 205, 581, 823, 1565
Psp124BI	GAGCTC	2 cut(s)	56, 1779
Psp6I	CCWGG	8 cut(s)	235, 410, 745, 873, 959, 1122, 1336, 1738
PspGI	CCWGG	8 cut(s)	235, 410, 745, 873, 959, 1122, 1336, 1738
PspN4I	GGNNCC	5 cut(s)	416, 990, 1391, 1790, 1897
PspPI	GGNCC	3 cut(s)	1550, 1714, 1930
PstI	CTGCAG	3 cut(s)	1018, 1507, 1964
PstNI	CAGNNNCTG	1 cut(s)	557
PsuI	RGATCY	5 cut(s)	793, 844, 901, 988, 1788
RsaI	GTAC	2 cut(s)	405, 1111
RsaNI	GTAC	2 cut(s)	404, 1110
RseI	CAYNNNNRTG	4 cut(s)	1117, 1139, 1803, 1970
SacI	GAGCTC	2 cut(s)	56, 1779
SalI	GTCGAC	1 cut(s)	59
SapI	GCTCTTC	1 cut(s)	1356
SatI	GCNGC	8 cut(s)	19, 755, 1014, 1266, 1506, 1587, 1960, 1963
Sau3AI	GATC	9 cut(s)	490, 631, 694, 793, 844, 901, 988, 1606, 1788
Sau96I	GGNCC	3 cut(s)	1550, 1714, 1930
SchI	GAGTC	6 cut(s)	113, 178, 206, 581, 823, 1565
ScrFI	CCNGG	9 cut(s)	237, 276, 412, 747, 875, 961, 1124, 1338, 1740
SduI	GDGCHC	5 cut(s)	56, 158, 319, 1647, 1779
SfaNI	GCATC	5 cut(s)	120, 254, 667, 1109, 1408
SfcI	CTRYAG	6 cut(s)	600, 820, 1014, 1260, 1503, 1960
SfoI	GGCGCC	1 cut(s)	1391
SfuI	TTCGAA	1 cut(s)	215
SgrDI	CGTCGACG	1 cut(s)	59
SinI	GGWCC	2 cut(s)	1550, 1714
SmiMI	CAYNNNNRTG	4 cut(s)	1117, 1139, 1803, 1970
SmlI	CTYRAG	7 cut(s)	589, 784, 797, 1061, 1085, 1204, 1280
SmoI	CTYRAG	7 cut(s)	589, 784, 797, 1061, 1085, 1204, 1280
SphI	GCATGC	1 cut(s)	1138
SseBI	AGGCCT	1 cut(s)	1743
SsiI	CCGC	4 cut(s)	18, 755, 1296, 1705
SspDI	GGCGCC	1 cut(s)	1389
SspI	AATATT	1 cut(s)	1290
SspMI	CTAG	6 cut(s)	191, 848, 996, 1353, 1563, 1839
SstI	GAGCTC	2 cut(s)	56, 1779
StuI	AGGCCT	1 cut(s)	1743
StyD4I	CCNGG	9 cut(s)	235, 274, 410, 745, 873, 959, 1122, 1336, 1738
StyI	CCWWGG	5 cut(s)	889, 1099, 1540, 1710, 1900
TaaI	ACNGT	8 cut(s)	45, 301, 883, 1235, 1277, 1729, 1908, 1946
TaiI	ACGT	2 cut(s)	1169, 2046
TaqI	TCGA	5 cut(s)	60, 114, 215, 227, 1984
TatI	WGTACW	1 cut(s)	403
TauI	GCSGC	2 cut(s)	21, 757
TfiI	GAWTC	7 cut(s)	212, 224, 244, 554, 742, 1409, 1691
TscAI	CASTG	6 cut(s)	142, 930, 1609, 1629, 1732, 1861
TseFI	GTSAC	1 cut(s)	2044
TseI	GCWGC	6 cut(s)	1013, 1265, 1505, 1586, 1959, 1962
Tsp45I	GTSAC	1 cut(s)	2044
TspDTI	ATGAA	4 cut(s)	234, 446, 896, 2081
TspGWI	ACGGA	4 cut(s)	46, 259, 1452, 1880
TspRI	CASTG	6 cut(s)	142, 930, 1609, 1629, 1732, 1861
Van91I	CCANNNNNTGG	1 cut(s)	1337
VneI	GTGCAC	1 cut(s)	315
VpaK11BI	GGWCC	2 cut(s)	1550, 1714
XapI	RAATTY	5 cut(s)	637, 1024, 1250, 2000, 2136
XceI	RCATGY	2 cut(s)	647, 1138
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1708, 1889
XmiI	GTMKAC	2 cut(s)	60, 1220
XmnI	GAANNNNTTC	1 cut(s)	309
XspI	CTAG	6 cut(s)	191, 848, 996, 1353, 1563, 1839
Zsp2I	ATGCAT	1 cut(s)	2083

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.