Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012217

Physical Location & Seq

Genomic Coordinates Reverse (-)

43475555 .. 43480013

Gene Structure 4459 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1302300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGGAGGGAGGCAGTGGTAAATCCATACTCCAATTCGCACCGTTTCAGAGCTCCGTCGACGAGGGTTTCTGGCACAGACTTTGTTCCTTGAAGCTCAACAAGTTCGGCGTCGACGACTCGCCTGTTCCCATCACCGGTTTCTACGCACCGTGCTCACATTCTCAAGTGTCTAATCATTTAACTCTAATGGCCGAGTCTTTGCCTTCGGATTCAAGTGAGGAATCATCGGTACAGGCAGTAAGTCGAGGCAATAGGAACAGATGCTCCGTTCCTGGAATCATTTACAACACCAATACGGTTGAAGGGTTTCATGCACTTGATAAACAGGGCTTGCTTAAGGCAGAAGCACAAAAGATTTGGGAGGATGTTCGTAGTGGAAAAGCATTGGAGGATAGTTCAGTGCTTTCGAGATTCCTCCTCATCTCTTTTGCAGACCTGAAAAAATGGAGCTTTCATTACTGGTTTGCATTCCCTGCTTTGGTGCTTGAACCTCCAGCAACCTTGGTTAATTTGAGGCCAGCTTCCCAATGTTTTAGCTCGGAGGAGGCAGAATCTGTTTCAGCAGCGTTTAACGAGTGGCGTAACTCATACCTAACAGCAGATGTGCCATTCTTCCTGGTTCATATTGATTCAAATTCACACGCTACTATTAAGCATCTAAAGGATTGGGAAACTTCCCAAAGTGCTGATGATGGTCACAAGTTACTATTTGGTTTTTATGATCCATGTCATCTTCCAAACCATCCCGGTTGGCCTCTTCGCAACCTCCTTGCACTTGCTGGCTCGAGATGGGATCTAAAGTCTGTTCACTTTTTATGCTATAGAGAGAGTCGTGGTTTTGCAGATCTGGGATTGTCCCTTGTTGGGGAAGCCATGATTACAATTCCGCCAGGATGGAGAAATCATGGTCATGTACTAAATGCAGTCGGGTGGGAACTTAACAAAGGGAGAAAAGTACCCAGGATAATAAGCCTTGCTAAATCTATGGATCCAACTAGGTTGGCCATATCTGCTGCAGATTTGAACTTGAAACTAATGAGGTGGCGTGCTTTGCCTTCTCTGAACTTAAATAGCTTGTCTTCTCTCAAGTGTCTCCTCCTTGGAGCTGGTACACTTGGATGCCAGGTTGCTCGCATGCTTATGGCTTGGGGTGTCCGGAACATTACGTTGGTTGACAATGGCAGGGTCGCTATGTCTAATCCTCTGAGGCAATCCTTGTATACGTTAGATGACTGCCTAAATGGCGGTGAATATAAAGCAACAGCAGCAGTTAATAGTCTCAAGAGAATCTTTCCAGCTGTGGAAGCAGAGGGTGTCATCATGGCTATACCAATGCCTGGACATCCTGTTCCGAGTCAAGAAGAGCAGAGTGTGCTTGATGATTGTAGACGACTAGATGATTTAATTGATTCTCACAATGTAGTTTTCCTGCTGACTGATACAAGAGAAAGTCGGTGGTTACCATCTCTCCTTTGTGCAAATACTAACAAGATTACTATAACTGCAGCTTTAGGGTTTGATAGCTTCTTGGTTATGCGCCATGGAGCAGGTCCTTGTAGCTCTAGCCATGACTCAAAAACTGAAGCTGAAAATTCTTTATCTGCGGATATGAGCAACCTTGGCCTTACCAATGGAAACGGAGGGAAAAGATTGGGCTGTTACTTCTGCAATGATGTCGTTGCGCCTATTGATTCAACTTCAAACCGCACGTTGGACCAGCAATGCACTGTTACACGTCCAGGCCTTGCTCTGATTGCCTCAGCTCTTGCAGTTGAGCTCTTAGTGGGGATCCTGCATCACCCTCATGGGATATTCGCGGAAGGTGAGGTGGTGAACTCCAGCAATAGTGGAAGCAGTGAGCAGCCTCTTGGTATTTTACCCCATCAAATTCGAGGTTCCCTCGCACAGTTTTCACAGATGACACTTGTGGGCCACTCCTCGGACAGTTGCACAGCTTGTTGCGGCACTGTGGTGTCTGAATATCGGAAAAGAGGAATGGAATTTATACTGCAAGCAATCAACCATCCTACATATCTGGAGGACCTAACTGGACTGACAGAGCTCATGAAATCAGCTAGTAAATTCGAGTTGGACTGGGACGATGGGACGGATGAAGACGATGATGATTTGGTTGAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000407 GO:0000422 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006497 GO:0006501 GO:0006807 GO:0006914 GO:0006950 GO:0006952 GO:0006995 GO:0006996 GO:0007005 GO:0007033 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008641 GO:0009056 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0009991 GO:0010150 GO:0016043 GO:0016236 GO:0016237 GO:0016740 GO:0016874 GO:0016877 GO:0018410 GO:0019538 GO:0019778 GO:0019779 GO:0022411 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0034645 GO:0034727 GO:0036211 GO:0042157 GO:0042158 GO:0042594 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043687 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044804 GO:0044805 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061726 GO:0061919 GO:0070925 GO:0071496 GO:0071704 GO:0071840 GO:0090693 GO:0098542 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1903008 GO:1905037

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 ko04216 map04136 map04138 map04140 map04216

Pfam Domains

Protein Families

ATG7_N ThiF

Protein Analysis

714

Amino Acids

78.03

Weight (kDa)

5.63

Isoelectric Point (pI)

46.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG7_N	PF16420	10 - 329	5.7e-101	Ubiquitin-like modifier-activating enzyme ATG7 N-terminus
ThiF	PF00899	346 - 601	1.3e-37	ThiF family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45900
fragaria_vesca	FvH4_3g07910
malus_domestica	MD05G1302300.v1.1 MD10G1281000.v1.1
prunus_persica	Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1
pyrus_communis	pycom05g27990 pycom10g23460
rosa_chinensis	RchiOBHm_Chr5g0010641
rosa_laevigata	RLG00000031768
rosa_multiflora	Rmu_sc0006103.1_g000002 Rmu_sc0014635.1_g000003
rosa_roxburghii	Rroxscaffold_1G00065730
rosa_rugosa	Rorug04G0451400.1
rosa_samantha	Rh5AG083000 Rh5CG091000
rosa_wichuraiana	Rw0G000680 Rw0G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1538
AccB7I	CCANNNNNTGG	1 cut(s)	1337
AccI	GTMKAC	4 cut(s)	57, 111, 1220, 1387
AccII	CGCG	1 cut(s)	1817
AccIII	TCCGGA	1 cut(s)	1155
AciI	CCGC	6 cut(s)	887, 1245, 1604, 1705, 1817, 1962
AclWI	GGATC	6 cut(s)	716, 801, 983, 996, 1783, 1796
AcoI	YGGCCR	2 cut(s)	189, 1002
AcsI	RAATTY	5 cut(s)	634, 1591, 1887, 2000, 2081
AcuI	CTGAAG	1 cut(s)	1602
AcyI	GRCGYC	1 cut(s)	108
AfaI	GTAC	4 cut(s)	231, 915, 957, 1111
AfiI	CCNNNNNNNGG	7 cut(s)	134, 478, 864, 865, 1337, 1711, 1939
AflII	CTTAAG	1 cut(s)	335
AflIII	ACRYGT	1 cut(s)	1733
AgeI	ACCGGT	1 cut(s)	134
AjiI	CACGTC	1 cut(s)	1736
AjnI	CCWGG	7 cut(s)	271, 615, 889, 959, 1122, 1336, 1738
AjuI	GAANNNNNNNTTGG	2 cut(s)	1151, 1183
AleI	CACNNNNGTG	1 cut(s)	1970
AloI	GAACNNNNNNTCC	4 cut(s)	248, 280, 1332, 1364
Alw21I	GWGCWC	4 cut(s)	53, 155, 1779, 2064
Alw26I	GTCTC	2 cut(s)	1097, 1283
AlwI	GGATC	6 cut(s)	716, 801, 983, 996, 1783, 1796
AlwNI	CAGNNNCTG	1 cut(s)	554
Ama87I	CYCGRG	1 cut(s)	784
Aor13HI	TCCGGA	1 cut(s)	1155
AoxI	GGCC	7 cut(s)	189, 515, 752, 1002, 1621, 1741, 1930
ApeKI	GCWGC	5 cut(s)	563, 1013, 1265, 1505, 1861
ApoI	RAATTY	5 cut(s)	634, 1591, 1887, 2000, 2081
AsiGI	ACCGGT	1 cut(s)	134
Asp700I	GAANNNNTTC	1 cut(s)	306
AspLEI	GCGC	2 cut(s)	1539, 1684
AspS9I	GGNCC	4 cut(s)	1550, 1714, 1930, 2041
AsuC2I	CCSGG	1 cut(s)	747
AsuHPI	GGTGA	5 cut(s)	124, 1259, 1790, 1835, 1843
AvaI	CYCGRG	1 cut(s)	784
AvaII	GGWCC	3 cut(s)	1550, 1714, 2041
BalI	TGGCCA	1 cut(s)	1004
BamHI	GGATCC	2 cut(s)	988, 1788
BanII	GRGCYC	3 cut(s)	53, 1779, 2064
BbsI	GAAGAC	2 cut(s)	1071, 2121
Bbv12I	GWGCWC	4 cut(s)	53, 155, 1779, 2064
BbvCI	CCTCAGC	1 cut(s)	1759
BbvI	GCAGC	5 cut(s)	575, 1000, 1277, 1517, 1873
BccI	CCATC	9 cut(s)	137, 686, 750, 783, 888, 1471, 1890, 2031, 2096
BcgI	CGANNNNNNTGC	2 cut(s)	1657, 1691
BciT130I	CCWGG	7 cut(s)	273, 617, 891, 961, 1124, 1338, 1740
BcnI	CCSGG	1 cut(s)	747
BcoDI	GTCTC	2 cut(s)	1097, 1283
BfaI	CTAG	4 cut(s)	996, 1394, 1563, 2076
BfmI	CTRYAG	3 cut(s)	820, 1014, 1503
BfrI	CTTAAG	1 cut(s)	335
BfuAI	ACCTGC	1 cut(s)	1538
BglI	GCCNNNNNGGC	1 cut(s)	1242
BglII	AGATCT	1 cut(s)	844
BisI	GCNGC	6 cut(s)	564, 1014, 1266, 1506, 1862, 1963
BlsI	GCNGC	6 cut(s)	565, 1015, 1267, 1507, 1863, 1964
Bme1390I	CCNGG	8 cut(s)	273, 617, 747, 891, 961, 1124, 1338, 1740
Bme18I	GGWCC	3 cut(s)	1550, 1714, 2041
BmeT110I	CYCGRG	1 cut(s)	784
BmgBI	CACGTC	1 cut(s)	1736
BmgT120I	GGNCC	4 cut(s)	1550, 1714, 1930, 2041
BmiI	GGNNCC	3 cut(s)	990, 1790, 1897
BmrFI	CCNGG	8 cut(s)	273, 617, 747, 891, 961, 1124, 1338, 1740
BmrI	ACTGGG	1 cut(s)	2104
BmsI	GCATC	4 cut(s)	251, 664, 1109, 1804
BmuI	ACTGGG	1 cut(s)	2104
BpiI	GAAGAC	2 cut(s)	1071, 2121
BplI	GAGNNNNNCTC	2 cut(s)	1884, 1916
BpmI	CTGGAG	3 cut(s)	477, 1822, 2057
Bpu10I	CCTNAGC	1 cut(s)	1759
BpuEI	CTTGAG	3 cut(s)	147, 1070, 1265
BpuMI	CCSGG	1 cut(s)	747
BsaHI	GRCGYC	1 cut(s)	108
BsaJI	CCNNGG	6 cut(s)	501, 959, 1099, 1540, 1618, 1938
BsaWI	WCCGGW	2 cut(s)	134, 1155
BsaXI	ACNNNNNCTCC	4 cut(s)	248, 278, 1452, 1482
Bsc4I	CCNNNNNNNGG	7 cut(s)	134, 478, 864, 865, 1337, 1711, 1939
Bse118I	RCCGGY	1 cut(s)	134
Bse1I	ACTGG	3 cut(s)	464, 2053, 2099
Bse3DI	GCAATG	2 cut(s)	1675, 1727
BseAI	TCCGGA	1 cut(s)	1155
BseBI	CCWGG	7 cut(s)	273, 617, 891, 961, 1124, 1338, 1740
BseDI	CCNNGG	6 cut(s)	501, 959, 1099, 1540, 1618, 1938
BseGI	GGATG	7 cut(s)	370, 742, 899, 1124, 1342, 2023, 2116
BseLI	CCNNNNNNNGG	7 cut(s)	134, 478, 864, 865, 1337, 1711, 1939
BseMI	GCAATG	2 cut(s)	1675, 1727
BseMII	CTCAG	2 cut(s)	1196, 1773
BseNI	ACTGG	3 cut(s)	464, 2053, 2099
BseRI	GAGGAG	4 cut(s)	407, 557, 1085, 1927
BseXI	GCAGC	5 cut(s)	575, 1000, 1277, 1517, 1873
Bsh1236I	CGCG	1 cut(s)	1817
BshFI	GGCC	7 cut(s)	191, 517, 754, 1004, 1623, 1743, 1932
BshTI	ACCGGT	1 cut(s)	134
BsiHKAI	GWGCWC	4 cut(s)	53, 155, 1779, 2064
BsiHKCI	CYCGRG	1 cut(s)	784
BsiSI	CCGG	3 cut(s)	135, 747, 1156
BslFI	GGGAC	3 cut(s)	841, 2111, 2119
BslI	CCNNNNNNNGG	7 cut(s)	134, 478, 864, 865, 1337, 1711, 1939
BsmAI	GTCTC	2 cut(s)	1097, 1283
BsmFI	GGGAC	3 cut(s)	841, 2111, 2119
BsmI	GAATGC	1 cut(s)	467
BsnI	GGCC	7 cut(s)	191, 517, 754, 1004, 1623, 1743, 1932
BsoBI	CYCGRG	1 cut(s)	784
Bsp1286I	GDGCHC	4 cut(s)	53, 155, 1779, 2064
Bsp13I	TCCGGA	1 cut(s)	1155
Bsp143I	GATC	5 cut(s)	721, 793, 844, 988, 1788
Bsp19I	CCATGG	1 cut(s)	1540
BspACI	CCGC	6 cut(s)	887, 1245, 1604, 1705, 1817, 1962
BspANI	GGCC	7 cut(s)	191, 517, 754, 1004, 1623, 1743, 1932
BspCNI	CTCAG	2 cut(s)	1197, 1772
BspEI	TCCGGA	1 cut(s)	1155
BspFNI	CGCG	1 cut(s)	1817
BspHI	TCATGA	1 cut(s)	2064
BspLI	GGNNCC	3 cut(s)	990, 1790, 1897
BspMAI	CTGCAG	2 cut(s)	1018, 1507
BspMI	ACCTGC	1 cut(s)	1538
BspPI	GGATC	6 cut(s)	716, 801, 983, 996, 1783, 1796
BspQI	GCTCTTC	1 cut(s)	1356
BspTI	CTTAAG	1 cut(s)	335
BsrDI	GCAATG	2 cut(s)	1675, 1727
BsrFI	RCCGGY	1 cut(s)	134
BsrI	ACTGG	3 cut(s)	464, 2053, 2099
BssAI	RCCGGY	1 cut(s)	134
BssECI	CCNNGG	6 cut(s)	501, 959, 1099, 1540, 1618, 1938
BssMI	GATC	5 cut(s)	721, 793, 844, 988, 1788
BssNAI	GTATAC	1 cut(s)	1221
BssNI	GRCGYC	1 cut(s)	108
BssT1I	CCWWGG	4 cut(s)	501, 1099, 1540, 1618
Bst1107I	GTATAC	1 cut(s)	1221
Bst2UI	CCWGG	7 cut(s)	273, 617, 891, 961, 1124, 1338, 1740
Bst4CI	ACNGT	7 cut(s)	42, 150, 298, 1729, 1908, 1946, 1969
Bst6I	CTCTTC	2 cut(s)	762, 1356
BstACI	GRCGYC	1 cut(s)	108
BstAFI	CTTAAG	1 cut(s)	335
BstAPI	GCANNNNNTGC	2 cut(s)	473, 1372
BstC8I	GCNNGC	7 cut(s)	332, 519, 781, 1047, 1132, 1136, 2013
BstDEI	CTNAG	3 cut(s)	1205, 1759, 1780
BstDSI	CCRYGG	1 cut(s)	1540
BstEII	GGTNACC	1 cut(s)	1458
BstF5I	GGATG	7 cut(s)	370, 742, 899, 1124, 1342, 2023, 2116
BstFNI	CGCG	1 cut(s)	1817
BstHHI	GCGC	2 cut(s)	1539, 1684
BstKTI	GATC	5 cut(s)	724, 796, 847, 991, 1791
BstMAI	GTCTC	2 cut(s)	1097, 1283
BstMBI	GATC	5 cut(s)	721, 793, 844, 988, 1788
BstMWI	GCNNNNNNNGC	9 cut(s)	473, 1010, 1051, 1242, 1265, 1304, 1372, 1620, 1754
BstNI	CCWGG	7 cut(s)	273, 617, 891, 961, 1124, 1338, 1740
BstNSI	RCATGY	1 cut(s)	1138
BstPI	GGTNACC	1 cut(s)	1458
BstSCI	CCNGG	8 cut(s)	271, 615, 745, 889, 959, 1122, 1336, 1738
BstSFI	CTRYAG	3 cut(s)	820, 1014, 1503
BstUI	CGCG	1 cut(s)	1817
BstV1I	GCAGC	5 cut(s)	575, 1000, 1277, 1517, 1873
BstV2I	GAAGAC	2 cut(s)	1071, 2121
BstX2I	RGATCY	4 cut(s)	793, 844, 988, 1788
BstYI	RGATCY	4 cut(s)	793, 844, 988, 1788
BstZ17I	GTATAC	1 cut(s)	1221
BsuRI	GGCC	7 cut(s)	191, 517, 754, 1004, 1623, 1743, 1932
BtgI	CCRYGG	1 cut(s)	1540
BtrI	CACGTC	1 cut(s)	1736
BtsCI	GGATG	7 cut(s)	370, 742, 899, 1124, 1342, 2023, 2116
BtsI	GCAGTG	2 cut(s)	19, 1861
BtsIMutI	CAGTG	5 cut(s)	19, 405, 1725, 1861, 1965
BveI	ACCTGC	1 cut(s)	1538
Cac8I	GCNNGC	7 cut(s)	332, 519, 781, 1047, 1132, 1136, 2013
CaiI	CAGNNNCTG	1 cut(s)	554
CciI	TCATGA	1 cut(s)	2064
CfoI	GCGC	2 cut(s)	1539, 1684
Cfr10I	RCCGGY	1 cut(s)	134
Cfr13I	GGNCC	4 cut(s)	1550, 1714, 1930, 2041
CseI	GACGC	1 cut(s)	97
Csp6I	GTAC	4 cut(s)	230, 914, 956, 1110
CspAI	ACCGGT	1 cut(s)	134
CviQI	GTAC	4 cut(s)	230, 914, 956, 1110
DdeI	CTNAG	3 cut(s)	1205, 1759, 1780
DpnI	GATC	5 cut(s)	723, 795, 846, 990, 1790
DpnII	GATC	5 cut(s)	721, 793, 844, 988, 1788
EaeI	YGGCCR	2 cut(s)	189, 1002
Eam1104I	CTCTTC	2 cut(s)	762, 1356
EarI	CTCTTC	2 cut(s)	762, 1356
EciI	GGCGGA	1 cut(s)	876
Ecl136II	GAGCTC	3 cut(s)	51, 1777, 2062
Eco130I	CCWWGG	4 cut(s)	501, 1099, 1540, 1618
Eco147I	AGGCCT	1 cut(s)	1743
Eco24I	GRGCYC	3 cut(s)	53, 1779, 2064
Eco47I	GGWCC	3 cut(s)	1550, 1714, 2041
Eco53kI	GAGCTC	3 cut(s)	51, 1777, 2062
Eco57I	CTGAAG	1 cut(s)	1602
Eco88I	CYCGRG	1 cut(s)	784
Eco91I	GGTNACC	1 cut(s)	1458
EcoICRI	GAGCTC	3 cut(s)	51, 1777, 2062
EcoO109I	RGGNCCY	2 cut(s)	1550, 2041
EcoO65I	GGTNACC	1 cut(s)	1458
EcoRII	CCWGG	7 cut(s)	271, 615, 889, 959, 1122, 1336, 1738
EcoT14I	CCWWGG	4 cut(s)	501, 1099, 1540, 1618
EcoT38I	GRGCYC	3 cut(s)	53, 1779, 2064
ErhI	CCWWGG	4 cut(s)	501, 1099, 1540, 1618
FalI	AAGNNNNNCTT	2 cut(s)	1274, 1306
FaqI	GGGAC	3 cut(s)	841, 2111, 2119
FblI	GTMKAC	4 cut(s)	57, 111, 1220, 1387
Fnu4HI	GCNGC	6 cut(s)	564, 1014, 1266, 1506, 1862, 1963
FokI	GGATG	7 cut(s)	377, 729, 906, 1131, 1329, 2010, 2123
FriOI	GRGCYC	3 cut(s)	53, 1779, 2064
Fsp4HI	GCNGC	6 cut(s)	564, 1014, 1266, 1506, 1862, 1963
FspBI	CTAG	4 cut(s)	996, 1394, 1563, 2076
GlaI	GCGC	2 cut(s)	1538, 1683
GluI	GCNGC	6 cut(s)	564, 1014, 1266, 1506, 1862, 1963
GsuI	CTGGAG	3 cut(s)	477, 1822, 2057
HaeIII	GGCC	7 cut(s)	191, 517, 754, 1004, 1623, 1743, 1932
HapII	CCGG	3 cut(s)	135, 747, 1156
HgaI	GACGC	1 cut(s)	97
HhaI	GCGC	2 cut(s)	1539, 1684
Hin1I	GRCGYC	1 cut(s)	108
Hin6I	GCGC	2 cut(s)	1537, 1682
HinP1I	GCGC	2 cut(s)	1537, 1682
HincII	GTYRAC	3 cut(s)	58, 112, 1174
HindII	GTYRAC	3 cut(s)	58, 112, 1174
HpaII	CCGG	3 cut(s)	135, 747, 1156
HphI	GGTGA	5 cut(s)	124, 1259, 1790, 1835, 1843
Hpy166II	GTNNAC	8 cut(s)	58, 112, 808, 1112, 1174, 1221, 1388, 1834
Hpy188III	TCNNGA	7 cut(s)	408, 786, 1156, 1282, 1358, 2036, 2065
Hpy8I	GTNNAC	8 cut(s)	58, 112, 808, 1112, 1174, 1221, 1388, 1834
Hpy99I	CGWCG	4 cut(s)	59, 62, 113, 116
HpyAV	CCTTC	4 cut(s)	213, 296, 1065, 1814
HpyCH4III	ACNGT	7 cut(s)	42, 150, 298, 1729, 1908, 1946, 1969
HpyCH4IV	ACGT	4 cut(s)	1166, 1223, 1709, 1735
HpyF10VI	GCNNNNNNNGC	9 cut(s)	473, 1010, 1051, 1242, 1265, 1304, 1372, 1620, 1754
HpyF3I	CTNAG	3 cut(s)	1205, 1759, 1780
HpySE526I	ACGT	4 cut(s)	1166, 1223, 1709, 1735
Hsp92I	GRCGYC	1 cut(s)	108
HspAI	GCGC	2 cut(s)	1537, 1682
Kpn2I	TCCGGA	1 cut(s)	1155
Kzo9I	GATC	5 cut(s)	721, 793, 844, 988, 1788
LguI	GCTCTTC	1 cut(s)	1356
LmnI	GCTCC	5 cut(s)	56, 269, 447, 1103, 1544
Lsp1109I	GCAGC	5 cut(s)	575, 1000, 1277, 1517, 1873
LweI	GCATC	4 cut(s)	251, 664, 1109, 1804
MaeI	CTAG	4 cut(s)	996, 1394, 1563, 2076
MaeII	ACGT	4 cut(s)	1166, 1223, 1709, 1735
MaeIII	GTNAC	6 cut(s)	581, 695, 702, 1458, 1658, 1729
MalI	GATC	5 cut(s)	723, 795, 846, 990, 1790
MboI	GATC	5 cut(s)	721, 793, 844, 988, 1788
MboII	GAAGA	6 cut(s)	604, 725, 749, 1071, 1373, 2126
MflI	RGATCY	4 cut(s)	793, 844, 988, 1788
MhlI	GDGCHC	4 cut(s)	53, 155, 1779, 2064
MlsI	TGGCCA	1 cut(s)	1004
MluCI	AATT	9 cut(s)	32, 508, 634, 882, 1404, 1591, 1887, 2000, 2081
MluNI	TGGCCA	1 cut(s)	1004
MlyI	GAGTC	5 cut(s)	110, 203, 838, 1363, 1565
MmeI	TCCRAC	3 cut(s)	1016, 1692, 2070
Mox20I	TGGCCA	1 cut(s)	1004
MroI	TCCGGA	1 cut(s)	1155
MroXI	GAANNNNTTC	1 cut(s)	306
MscI	TGGCCA	1 cut(s)	1004
MseI	TTAA	9 cut(s)	179, 336, 507, 570, 651, 939, 1067, 1272, 1403
MslI	CAYNNNNRTG	5 cut(s)	909, 1117, 1139, 1803, 1970
Msp20I	TGGCCA	1 cut(s)	1004
MspA1I	CMGCKG	1 cut(s)	1298
MspCI	CTTAAG	1 cut(s)	335
MspI	CCGG	3 cut(s)	135, 747, 1156
MspR9I	CCNGG	8 cut(s)	273, 617, 747, 891, 961, 1124, 1338, 1740
Mva1269I	GAATGC	1 cut(s)	467
MvaI	CCWGG	7 cut(s)	273, 617, 891, 961, 1124, 1338, 1740
MvnI	CGCG	1 cut(s)	1817
MwoI	GCNNNNNNNGC	9 cut(s)	473, 1010, 1051, 1242, 1265, 1304, 1372, 1620, 1754
NciI	CCSGG	1 cut(s)	747
NcoI	CCATGG	1 cut(s)	1540
NdeII	GATC	5 cut(s)	721, 793, 844, 988, 1788
NlaIV	GGNNCC	3 cut(s)	990, 1790, 1897
NmeAIII	GCCGAG	1 cut(s)	217
NmuCI	GTSAC	1 cut(s)	695
NspI	RCATGY	1 cut(s)	1138
OliI	CACNNNNGTG	1 cut(s)	1970
PaeI	GCATGC	1 cut(s)	1138
PaeR7I	CTCGAG	1 cut(s)	784
PagI	TCATGA	1 cut(s)	2064
PceI	AGGCCT	1 cut(s)	1743
PciSI	GCTCTTC	1 cut(s)	1356
PcsI	WCGNNNNNNNCGW	1 cut(s)	111
PctI	GAATGC	1 cut(s)	467
PdmI	GAANNNNTTC	1 cut(s)	306
PfeI	GAWTC	9 cut(s)	209, 221, 276, 411, 551, 629, 1287, 1409, 1691
PflMI	CCANNNNNTGG	1 cut(s)	1337
PfoI	TCCNGGA	1 cut(s)	271
PinAI	ACCGGT	1 cut(s)	134
PkrI	GCNGC	6 cut(s)	565, 1015, 1267, 1507, 1863, 1964
PleI	GAGTC	5 cut(s)	110, 202, 837, 1362, 1565
PpsI	GAGTC	5 cut(s)	110, 202, 837, 1362, 1565
PpuMI	RGGWCCY	2 cut(s)	1550, 2041
Psp124BI	GAGCTC	3 cut(s)	53, 1779, 2064
Psp5II	RGGWCCY	2 cut(s)	1550, 2041
Psp6I	CCWGG	7 cut(s)	271, 615, 889, 959, 1122, 1336, 1738
PspEI	GGTNACC	1 cut(s)	1458
PspGI	CCWGG	7 cut(s)	271, 615, 889, 959, 1122, 1336, 1738
PspN4I	GGNNCC	3 cut(s)	990, 1790, 1897
PspPI	GGNCC	4 cut(s)	1550, 1714, 1930, 2041
PspPPI	RGGWCCY	2 cut(s)	1550, 2041
PstI	CTGCAG	2 cut(s)	1018, 1507
PstNI	CAGNNNCTG	1 cut(s)	554
PsuI	RGATCY	4 cut(s)	793, 844, 988, 1788
PvuII	CAGCTG	1 cut(s)	1298
RsaI	GTAC	4 cut(s)	231, 915, 957, 1111
RsaNI	GTAC	4 cut(s)	230, 914, 956, 1110
RseI	CAYNNNNRTG	5 cut(s)	909, 1117, 1139, 1803, 1970
SacI	GAGCTC	3 cut(s)	53, 1779, 2064
SalI	GTCGAC	2 cut(s)	56, 110
SapI	GCTCTTC	1 cut(s)	1356
SaqAI	TTAA	9 cut(s)	179, 336, 507, 570, 651, 939, 1067, 1272, 1403
SatI	GCNGC	6 cut(s)	564, 1014, 1266, 1506, 1862, 1963
Sau3AI	GATC	5 cut(s)	721, 793, 844, 988, 1788
Sau96I	GGNCC	4 cut(s)	1550, 1714, 1930, 2041
SchI	GAGTC	5 cut(s)	110, 203, 838, 1363, 1565
ScrFI	CCNGG	8 cut(s)	273, 617, 747, 891, 961, 1124, 1338, 1740
SduI	GDGCHC	4 cut(s)	53, 155, 1779, 2064
SfaNI	GCATC	4 cut(s)	251, 664, 1109, 1804
SfcI	CTRYAG	3 cut(s)	820, 1014, 1503
Sfr274I	CTCGAG	1 cut(s)	784
SgrDI	CGTCGACG	2 cut(s)	56, 110
SinI	GGWCC	3 cut(s)	1550, 1714, 2041
SlaI	CTCGAG	1 cut(s)	784
SmiMI	CAYNNNNRTG	5 cut(s)	909, 1117, 1139, 1803, 1970
SmlI	CTYRAG	5 cut(s)	162, 335, 784, 1085, 1280
SmoI	CTYRAG	5 cut(s)	162, 335, 784, 1085, 1280
SphI	GCATGC	1 cut(s)	1138
Sse9I	AATT	9 cut(s)	32, 508, 634, 882, 1404, 1591, 1887, 2000, 2081
SseBI	AGGCCT	1 cut(s)	1743
SsiI	CCGC	6 cut(s)	887, 1245, 1604, 1705, 1817, 1962
SspMI	CTAG	4 cut(s)	996, 1394, 1563, 2076
SstI	GAGCTC	3 cut(s)	53, 1779, 2064
StuI	AGGCCT	1 cut(s)	1743
StyD4I	CCNGG	8 cut(s)	271, 615, 745, 889, 959, 1122, 1336, 1738
StyI	CCWWGG	4 cut(s)	501, 1099, 1540, 1618
TaaI	ACNGT	7 cut(s)	42, 150, 298, 1729, 1908, 1946, 1969
TaiI	ACGT	4 cut(s)	1169, 1226, 1712, 1738
TaqI	TCGA	7 cut(s)	57, 111, 244, 407, 785, 1891, 2085
TasI	AATT	9 cut(s)	32, 508, 634, 882, 1404, 1591, 1887, 2000, 2081
TatI	WGTACW	1 cut(s)	913
TauI	GCSGC	1 cut(s)	1965
TfiI	GAWTC	9 cut(s)	209, 221, 276, 411, 551, 629, 1287, 1409, 1691
Tru1I	TTAA	9 cut(s)	179, 336, 507, 570, 651, 939, 1067, 1272, 1403
Tru9I	TTAA	9 cut(s)	179, 336, 507, 570, 651, 939, 1067, 1272, 1403
TscAI	CASTG	5 cut(s)	19, 405, 1732, 1861, 1972
TseFI	GTSAC	1 cut(s)	695
TseI	GCWGC	5 cut(s)	563, 1013, 1265, 1505, 1861
Tsp45I	GTSAC	1 cut(s)	695
TspDTI	ATGAA	5 cut(s)	299, 443, 611, 2081, 2127
TspGWI	ACGGA	4 cut(s)	43, 256, 1653, 2123
TspRI	CASTG	5 cut(s)	19, 405, 1732, 1861, 1972
Van91I	CCANNNNNTGG	1 cut(s)	1337
Vha464I	CTTAAG	1 cut(s)	335
VpaK11BI	GGWCC	3 cut(s)	1550, 1714, 2041
XapI	RAATTY	5 cut(s)	634, 1591, 1887, 2000, 2081
XceI	RCATGY	1 cut(s)	1138
XhoI	CTCGAG	1 cut(s)	784
XmiI	GTMKAC	4 cut(s)	57, 111, 1220, 1387
XmnI	GAANNNNTTC	1 cut(s)	306
XspI	CTAG	4 cut(s)	996, 1394, 1563, 2076

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.