Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0012217

Physical Location & Seq

Genomic Coordinates Reverse (-)

87315939 .. 87324728

Gene Structure 8790 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00065730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2145 bp

ATGGAGGCGAAGAATAGCGGCAAACCCATACTTCAATTCGCACCGTTTCAGAGCTCCGTCGACGAGGGCTTCTGGCACAGATTGTCTTCTTTGAAGCTCAACAAGTTTGGCATCGACGACTCTCCAATTCCCATCACTGGGTTCTATGCACCGTGCTCACATTCTCAGGTATCAAATCATTTGACTCTTCTAGCTGAGTCTTTGCCTTCGGATTCGAATGAAGAATCGAGTGAACCAGGAGTGATTCGGGGAAATAGGAATAGATGCTCCGTTCCCGGCATTCTTTACAACACCAATACAGTAGAAGGCTTTCGTGCACTTGACAAACAGGAGTTGATTAAAGCAGAAGCGAAAAAGATTTGGGAGGACATTCAGAATGGAAAAGTTTTGGAGGATAGTTCTGTACTTTCCAGGTTCCTCCTTATCTCTTTTGCAGACCTTAAAAAATGGAGCTTTCATTACTGGTTTGCCTTCCCTGCTCTGGTGCTTGATCCTCCAGCAACATTGCTTGATTTGAAACCAGCTTCACAATTTTTTAGTGTGAAGGAGGCAGAATCTGTCTCAGCAGTATTAAATGACTGGCGCGACTCAAGCTTAACTACAGATGTGCCGTTCTTTTTGGTTGTAATTGATCCAAATTCGCATGCTACCATTAAGCATCTAAAGGATTGGGAAACTTGTCAAAGAGCTACTGATCACAAGGTCTTTGCTATTCGGTTTTACGACCCATGCCATCTTCCAAAGAATCCTGGTTGGCCGCTTCGGAACTTGCTTGCACTTATTTGCTCAAGATGGGATCTCAAGTCTGTTCGCTTTCTATGCTATAGAGAGACTCGTGGTTTCGCAGATCTAGGATTGTCCCTTGTTGGTGAAGCCCAGGTTACAGTTCCCCAAGGATGGGGAGATCTTCATAATACACCCAATGCAGTGGGGTGGGAACTTAACAAAGGGAAAAAAATACCCAGGTGTATTAGCCTTGCTAAATCTATGGATCCAATTAGGTTGGCCGTATCTGCTGCAGATTTGAATTTGAAACTAATGAGATGGCGTGCTTTACCTTCTCTCAACTTAAATACCTTGTCCTCTCTCAAGTGTCTCCTCCTTGGAGCAGGTACACTTGGATGCCAGGTTGCTCGCATGCTTATGGCTTGGGGCGTTCGGAAAATTACGTTGGTTGACAATGGCAGAGTGGCTATGTCTAATCCCTTGAGGCAATCCCTGTATACATTAGATGACTGTCTAAATGGTGGTGAATTTAAAGCTGTAGCAGCAGTTAACAGTCTCAAGAAAATATTTCCCGCTGTGGAAGCAGAGGGTGTTGTCATGGCTATACCAATGCCTGGACATCCCGTTCCTAGTCAAGAAGAGCAGAGTGTGCTTAATGATTGTAGGCACCTACATGATTTAATTGATTCTCATGATGCAGTTTTCTTGTTAACTGATACAAGAGAAAGTCGGTGGCTTCCGTCTCTTCTTTGTGCCAATACTAACAAGATTACTATAACTGCAGCTCTTGGGTTTGATAGCTTTTTAGTTATGCGCCATGGAGCAGGTCCATGCAGCTCTAGCGATGACTCAAAATCTGAAGCTGCAAATGCTTTATCTACTGATCTGAATAGCCTTGCACTGACTGATAGAAATGGAGGGCACAGATTGGGTTGCTACTTCTGCAATGATGTGGTTGCACCTATTGATTCAACTGCCAACCGCACCTTGGACCAGCAATGCACTGTTACACACCCAGGCCTCGCTCCTATTGCCTCAGCTCTTGCAGTTGAGCTCTTAGTTGGGATCCTACATCACCCTGATGGGATATTTGCCGAAGGTGAAATTGCAAACTCTAGTAATAATGGAAGCAGTGAGCAACCTCTTGGTATTTTACCCCATCAAATCCGTGGTTCCCTTGGACAGTTTTCGCAGATGACACTTGTGGGCCACTCCTCAGACAGTTGCACGGCTTGCTGCAGCATTGTGGTGTCTGAATATCGAAAAAGGGGAATGGAATTTATACTACAAGCAATCAACCATCCCACCTATCTGGAGGACGTGACTGGACTAACAGAGTTGATGAAAACAGCTCATGCATTTCAAATGGATTGGGATGATGGGACTGATAATGATGATGATGATTGTGTTGAAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000407 GO:0000422 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006497 GO:0006501 GO:0006807 GO:0006914 GO:0006950 GO:0006952 GO:0006995 GO:0006996 GO:0007005 GO:0007033 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008641 GO:0009056 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0009991 GO:0010150 GO:0016043 GO:0016236 GO:0016237 GO:0016740 GO:0016874 GO:0016877 GO:0018410 GO:0019538 GO:0019778 GO:0019779 GO:0022411 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0034645 GO:0034727 GO:0036211 GO:0042157 GO:0042158 GO:0042594 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043687 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044804 GO:0044805 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061726 GO:0061919 GO:0070925 GO:0071496 GO:0071704 GO:0071840 GO:0090693 GO:0098542 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1903008 GO:1905037

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 ko04216 map04136 map04138 map04140 map04216

Pfam Domains

Protein Families

ATG7_N ThiF

Protein Analysis

714

Amino Acids

78.45

Weight (kDa)

5.66

Isoelectric Point (pI)

37.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG7_N	PF16420	11 - 330	8.8e-102	Ubiquitin-like modifier-activating enzyme ATG7 N-terminus
ThiF	PF00899	347 - 603	1.7e-37	ThiF family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45900
fragaria_vesca	FvH4_3g07910
malus_domestica	MD05G1302300.v1.1 MD10G1281000.v1.1
prunus_persica	Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1 Prupe.4G060100_v2.0.a1
pyrus_communis	pycom05g27990 pycom10g23460
rosa_chinensis	RchiOBHm_Chr5g0010641
rosa_laevigata	RLG00000031768
rosa_multiflora	Rmu_sc0006103.1_g000002 Rmu_sc0014635.1_g000003
rosa_roxburghii	Rroxscaffold_1G00065730
rosa_rugosa	Rorug04G0451400.1
rosa_samantha	Rh5AG083000 Rh5CG091000
rosa_wichuraiana	Rw0G000680 Rw0G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1100, 1541
AccB1I	GGYRCC	1 cut(s)	1392
AccB7I	CCANNNNNTGG	1 cut(s)	1340
AccI	GTMKAC	2 cut(s)	60, 1223
AccII	CGCG	1 cut(s)	585
AciI	CCGC	4 cut(s)	18, 758, 1299, 1708
AclWI	GGATC	7 cut(s)	485, 626, 804, 986, 999, 1786, 1799
AcoI	YGGCCR	2 cut(s)	755, 1005
AcsI	RAATTY	5 cut(s)	637, 1027, 1253, 2003, 2139
AcuI	CTGAAG	1 cut(s)	1605
AfaI	GTAC	2 cut(s)	405, 1114
AfiI	CCNNNNNNNGG	7 cut(s)	137, 138, 481, 897, 898, 1340, 1714
AgsI	TTSAA	8 cut(s)	35, 94, 517, 1027, 1033, 1698, 2090, 2138
AjiI	CACGTC	1 cut(s)	2047
AjnI	CCWGG	8 cut(s)	235, 410, 748, 876, 962, 1125, 1339, 1741
AjuI	GAANNNNNNNTTGG	2 cut(s)	1154, 1186
AloI	GAACNNNNNNTCC	2 cut(s)	1335, 1367
Alw21I	GWGCWC	4 cut(s)	56, 158, 319, 1782
Alw26I	GTCTC	5 cut(s)	565, 824, 1100, 1286, 1473
Alw44I	GTGCAC	1 cut(s)	315
AlwI	GGATC	7 cut(s)	485, 626, 804, 986, 999, 1786, 1799
AlwNI	CAGNNNCTG	1 cut(s)	557
AoxI	GGCC	4 cut(s)	755, 1005, 1744, 1933
ApaLI	GTGCAC	1 cut(s)	315
ApeKI	GCWGC	7 cut(s)	1016, 1268, 1508, 1560, 1589, 1962, 1965
ApoI	RAATTY	5 cut(s)	637, 1027, 1253, 2003, 2139
Asp700I	GAANNNNTTC	1 cut(s)	309
AspLEI	GCGC	2 cut(s)	585, 1542
AspS9I	GGNCC	3 cut(s)	1553, 1717, 1933
AsuC2I	CCSGG	1 cut(s)	276
AsuHPI	GGTGA	4 cut(s)	881, 1262, 1793, 1838
AsuII	TTCGAA	1 cut(s)	215
AvaII	GGWCC	2 cut(s)	1553, 1717
BaeGI	GKGCMC	2 cut(s)	319, 1650
BamHI	GGATCC	2 cut(s)	991, 1791
BanI	GGYRCC	1 cut(s)	1392
BanII	GRGCYC	2 cut(s)	56, 1782
BauI	CACGAG	1 cut(s)	834
BbsI	GAAGAC	1 cut(s)	78
Bbv12I	GWGCWC	4 cut(s)	56, 158, 319, 1782
BbvCI	CCTCAGC	1 cut(s)	1762
BbvI	GCAGC	7 cut(s)	1003, 1280, 1520, 1572, 1576, 1949, 1977
BccI	CCATC	9 cut(s)	140, 741, 786, 891, 1038, 1802, 1893, 2034, 2099
BceAI	ACGGC	3 cut(s)	595, 992, 1971
BciT130I	CCWGG	8 cut(s)	237, 412, 750, 878, 964, 1127, 1341, 1743
BclI	TGATCA	1 cut(s)	694
BcnI	CCSGG	1 cut(s)	276
BcoDI	GTCTC	5 cut(s)	565, 824, 1100, 1286, 1473
BfaI	CTAG	5 cut(s)	191, 851, 1356, 1566, 1842
BfmI	CTRYAG	6 cut(s)	600, 823, 1017, 1263, 1506, 1963
BfuAI	ACCTGC	2 cut(s)	1100, 1541
BglII	AGATCT	2 cut(s)	847, 904
BisI	GCNGC	9 cut(s)	19, 758, 1017, 1269, 1509, 1561, 1590, 1963, 1966
BlsI	GCNGC	9 cut(s)	20, 759, 1018, 1270, 1510, 1562, 1591, 1964, 1967
Bme1390I	CCNGG	9 cut(s)	237, 276, 412, 750, 878, 964, 1127, 1341, 1743
Bme18I	GGWCC	2 cut(s)	1553, 1717
BmgBI	CACGTC	1 cut(s)	2047
BmgT120I	GGNCC	3 cut(s)	1553, 1717, 1933
BmiI	GGNNCC	5 cut(s)	416, 993, 1394, 1793, 1900
BmrFI	CCNGG	9 cut(s)	237, 276, 412, 750, 878, 964, 1127, 1341, 1743
BmrI	ACTGGG	1 cut(s)	147
BmsI	GCATC	5 cut(s)	120, 254, 667, 1112, 1411
BmuI	ACTGGG	1 cut(s)	147
BpiI	GAAGAC	1 cut(s)	78
BpmI	CTGGAG	2 cut(s)	480, 2060
Bpu10I	CCTNAGC	1 cut(s)	1762
Bpu14I	TTCGAA	1 cut(s)	215
BpuEI	CTTGAG	6 cut(s)	574, 772, 785, 1073, 1228, 1268
BpuMI	CCSGG	1 cut(s)	276
BsaJI	CCNNGG	9 cut(s)	876, 892, 962, 1102, 1543, 1713, 1741, 1894, 1903
Bsc4I	CCNNNNNNNGG	7 cut(s)	137, 138, 481, 897, 898, 1340, 1714
Bse1I	ACTGG	4 cut(s)	142, 467, 584, 2056
Bse3DI	GCAATG	3 cut(s)	503, 1678, 1730
BseBI	CCWGG	8 cut(s)	237, 412, 750, 878, 964, 1127, 1341, 1743
BseDI	CCNNGG	9 cut(s)	876, 892, 962, 1102, 1543, 1713, 1741, 1894, 1903
BseGI	GGATG	5 cut(s)	902, 1127, 1345, 2026, 2107
BseLI	CCNNNNNNNGG	7 cut(s)	137, 138, 481, 897, 898, 1340, 1714
BseMI	GCAATG	3 cut(s)	503, 1678, 1730
BseMII	CTCAG	5 cut(s)	179, 186, 576, 1776, 1956
BseNI	ACTGG	4 cut(s)	142, 467, 584, 2056
BseRI	GAGGAG	2 cut(s)	1088, 1930
BseSI	GKGCMC	2 cut(s)	319, 1650
BseXI	GCAGC	7 cut(s)	1003, 1280, 1520, 1572, 1576, 1949, 1977
Bsh1236I	CGCG	1 cut(s)	585
BshFI	GGCC	4 cut(s)	757, 1007, 1746, 1935
BshNI	GGYRCC	1 cut(s)	1392
BsiHKAI	GWGCWC	4 cut(s)	56, 158, 319, 1782
BsiSI	CCGG	1 cut(s)	276
BslFI	GGGAC	2 cut(s)	844, 2122
BslI	CCNNNNNNNGG	7 cut(s)	137, 138, 481, 897, 898, 1340, 1714
BsmAI	GTCTC	5 cut(s)	565, 824, 1100, 1286, 1473
BsmBI	CGTCTC	1 cut(s)	1473
BsmFI	GGGAC	2 cut(s)	844, 2122
BsmI	GAATGC	1 cut(s)	279
BsnI	GGCC	4 cut(s)	757, 1007, 1746, 1935
Bsp119I	TTCGAA	1 cut(s)	215
Bsp1286I	GDGCHC	5 cut(s)	56, 158, 319, 1650, 1782
Bsp143I	GATC	9 cut(s)	490, 631, 694, 796, 847, 904, 991, 1609, 1791
Bsp19I	CCATGG	1 cut(s)	1543
BspACI	CCGC	4 cut(s)	18, 758, 1299, 1708
BspANI	GGCC	4 cut(s)	757, 1007, 1746, 1935
BspCNI	CTCAG	5 cut(s)	178, 187, 575, 1775, 1955
BspFNI	CGCG	1 cut(s)	585
BspHI	TCATGA	1 cut(s)	1417
BspLI	GGNNCC	5 cut(s)	416, 993, 1394, 1793, 1900
BspMAI	CTGCAG	3 cut(s)	1021, 1510, 1967
BspMI	ACCTGC	2 cut(s)	1100, 1541
BspPI	GGATC	7 cut(s)	485, 626, 804, 986, 999, 1786, 1799
BspQI	GCTCTTC	1 cut(s)	1359
BspT104I	TTCGAA	1 cut(s)	215
BspT107I	GGYRCC	1 cut(s)	1392
BsrDI	GCAATG	3 cut(s)	503, 1678, 1730
BsrI	ACTGG	4 cut(s)	142, 467, 584, 2056
BssECI	CCNNGG	9 cut(s)	876, 892, 962, 1102, 1543, 1713, 1741, 1894, 1903
BssMI	GATC	9 cut(s)	490, 631, 694, 796, 847, 904, 991, 1609, 1791
BssNAI	GTATAC	1 cut(s)	1224
BssSI	CACGAG	1 cut(s)	834
BssT1I	CCWWGG	5 cut(s)	892, 1102, 1543, 1713, 1903
Bst1107I	GTATAC	1 cut(s)	1224
Bst2BI	CACGAG	1 cut(s)	834
Bst2UI	CCWGG	8 cut(s)	237, 412, 750, 878, 964, 1127, 1341, 1743
Bst4CI	ACNGT	9 cut(s)	45, 153, 301, 886, 1238, 1280, 1732, 1911, 1949
Bst6I	CTCTTC	3 cut(s)	192, 1359, 1476
BstAPI	GCANNNNNTGC	2 cut(s)	1375, 1959
BstBI	TTCGAA	1 cut(s)	215
BstC8I	GCNNGC	6 cut(s)	645, 774, 1050, 1135, 1139, 1960
BstDEI	CTNAG	6 cut(s)	165, 195, 562, 1762, 1783, 1942
BstDSI	CCRYGG	2 cut(s)	1543, 1894
BstF5I	GGATG	5 cut(s)	902, 1127, 1345, 2026, 2107
BstFNI	CGCG	1 cut(s)	585
BstHHI	GCGC	2 cut(s)	585, 1542
BstKTI	GATC	9 cut(s)	493, 634, 697, 799, 850, 907, 994, 1612, 1794
BstMAI	GTCTC	5 cut(s)	565, 824, 1100, 1286, 1473
BstMBI	GATC	9 cut(s)	490, 631, 694, 796, 847, 904, 991, 1609, 1791
BstNI	CCWGG	8 cut(s)	237, 412, 750, 878, 964, 1127, 1341, 1743
BstNSI	RCATGY	2 cut(s)	647, 1141
BstSCI	CCNGG	9 cut(s)	235, 274, 410, 748, 876, 962, 1125, 1339, 1741
BstSFI	CTRYAG	6 cut(s)	600, 823, 1017, 1263, 1506, 1963
BstSLI	GKGCMC	2 cut(s)	319, 1650
BstUI	CGCG	1 cut(s)	585
BstV1I	GCAGC	7 cut(s)	1003, 1280, 1520, 1572, 1576, 1949, 1977
BstV2I	GAAGAC	1 cut(s)	78
BstX2I	RGATCY	5 cut(s)	796, 847, 904, 991, 1791
BstXI	CCANNNNNNTGG	2 cut(s)	928, 2038
BstYI	RGATCY	5 cut(s)	796, 847, 904, 991, 1791
BstZ17I	GTATAC	1 cut(s)	1224
BsuRI	GGCC	4 cut(s)	757, 1007, 1746, 1935
BtgI	CCRYGG	2 cut(s)	1543, 1894
BtgZI	GCGATG	1 cut(s)	1584
BtrI	CACGTC	1 cut(s)	2047
BtsCI	GGATG	5 cut(s)	902, 1127, 1345, 2026, 2107
BtsI	GCAGTG	2 cut(s)	933, 1864
BtsIMutI	CAGTG	5 cut(s)	135, 933, 1625, 1728, 1864
BveI	ACCTGC	2 cut(s)	1100, 1541
Cac8I	GCNNGC	6 cut(s)	645, 774, 1050, 1135, 1139, 1960
CaiI	CAGNNNCTG	1 cut(s)	557
CciI	TCATGA	1 cut(s)	1417
CfoI	GCGC	2 cut(s)	585, 1542
Cfr13I	GGNCC	3 cut(s)	1553, 1717, 1933
Csp6I	GTAC	2 cut(s)	404, 1113
CviAII	CATG	9 cut(s)	644, 729, 1138, 1324, 1400, 1418, 1544, 1557, 2081
CviQI	GTAC	2 cut(s)	404, 1113
DdeI	CTNAG	6 cut(s)	165, 195, 562, 1762, 1783, 1942
DpnI	GATC	9 cut(s)	492, 633, 696, 798, 849, 906, 993, 1611, 1793
DpnII	GATC	9 cut(s)	490, 631, 694, 796, 847, 904, 991, 1609, 1791
DraI	TTTAAA	1 cut(s)	1258
EaeI	YGGCCR	2 cut(s)	755, 1005
Eam1104I	CTCTTC	3 cut(s)	192, 1359, 1476
EarI	CTCTTC	3 cut(s)	192, 1359, 1476
Ecl136II	GAGCTC	2 cut(s)	54, 1780
Eco130I	CCWWGG	5 cut(s)	892, 1102, 1543, 1713, 1903
Eco147I	AGGCCT	1 cut(s)	1746
Eco24I	GRGCYC	2 cut(s)	56, 1782
Eco47I	GGWCC	2 cut(s)	1553, 1717
Eco53kI	GAGCTC	2 cut(s)	54, 1780
Eco57I	CTGAAG	1 cut(s)	1605
EcoICRI	GAGCTC	2 cut(s)	54, 1780
EcoRII	CCWGG	8 cut(s)	235, 410, 748, 876, 962, 1125, 1339, 1741
EcoT14I	CCWWGG	5 cut(s)	892, 1102, 1543, 1713, 1903
EcoT22I	ATGCAT	1 cut(s)	2086
EcoT38I	GRGCYC	2 cut(s)	56, 1782
ErhI	CCWWGG	5 cut(s)	892, 1102, 1543, 1713, 1903
Esp3I	CGTCTC	1 cut(s)	1473
FaeI	CATG	9 cut(s)	647, 732, 1141, 1327, 1403, 1421, 1547, 1560, 2084
FaqI	GGGAC	2 cut(s)	844, 2122
FatI	CATG	9 cut(s)	643, 728, 1137, 1323, 1399, 1417, 1543, 1556, 2080
FauI	CCCGC	1 cut(s)	1306
FbaI	TGATCA	1 cut(s)	694
FblI	GTMKAC	2 cut(s)	60, 1223
Fnu4HI	GCNGC	9 cut(s)	19, 758, 1017, 1269, 1509, 1561, 1590, 1963, 1966
FokI	GGATG	5 cut(s)	909, 1134, 1332, 2013, 2114
FriOI	GRGCYC	2 cut(s)	56, 1782
Fsp4HI	GCNGC	9 cut(s)	19, 758, 1017, 1269, 1509, 1561, 1590, 1963, 1966
FspBI	CTAG	5 cut(s)	191, 851, 1356, 1566, 1842
GlaI	GCGC	2 cut(s)	584, 1541
GluI	GCNGC	9 cut(s)	19, 758, 1017, 1269, 1509, 1561, 1590, 1963, 1966
GsuI	CTGGAG	2 cut(s)	480, 2060
HaeIII	GGCC	4 cut(s)	757, 1007, 1746, 1935
HapII	CCGG	1 cut(s)	276
HhaI	GCGC	2 cut(s)	585, 1542
Hin1II	CATG	9 cut(s)	647, 732, 1141, 1327, 1403, 1421, 1547, 1560, 2084
Hin6I	GCGC	2 cut(s)	583, 1540
HinP1I	GCGC	2 cut(s)	583, 1540
HincII	GTYRAC	4 cut(s)	61, 1177, 1276, 1437
HindII	GTYRAC	4 cut(s)	61, 1177, 1276, 1437
HindIII	AAGCTT	1 cut(s)	592
HpaI	GTTAAC	2 cut(s)	1276, 1437
HpaII	CCGG	1 cut(s)	276
HphI	GGTGA	4 cut(s)	881, 1262, 1793, 1838
Hpy166II	GTNNAC	8 cut(s)	61, 233, 317, 1115, 1177, 1224, 1276, 1437
Hpy188I	TCNGA	9 cut(s)	51, 211, 375, 765, 1161, 1585, 1614, 1945, 1981
Hpy188III	TCNNGA	5 cut(s)	789, 1285, 1361, 1418, 2039
Hpy8I	GTNNAC	8 cut(s)	61, 233, 317, 1115, 1177, 1224, 1276, 1437
Hpy99I	CGWCG	3 cut(s)	62, 65, 119
HpyAV	CCTTC	6 cut(s)	216, 299, 481, 538, 1068, 1817
HpyCH4III	ACNGT	9 cut(s)	45, 153, 301, 886, 1238, 1280, 1732, 1911, 1949
HpyCH4IV	ACGT	2 cut(s)	1169, 2046
HpyF3I	CTNAG	6 cut(s)	165, 195, 562, 1762, 1783, 1942
HpySE526I	ACGT	2 cut(s)	1169, 2046
Hsp92II	CATG	9 cut(s)	647, 732, 1141, 1327, 1403, 1421, 1547, 1560, 2084
HspAI	GCGC	2 cut(s)	583, 1540
Ksp22I	TGATCA	1 cut(s)	694
KspAI	GTTAAC	2 cut(s)	1276, 1437
Kzo9I	GATC	9 cut(s)	490, 631, 694, 796, 847, 904, 991, 1609, 1791
LguI	GCTCTTC	1 cut(s)	1359
LmnI	GCTCC	6 cut(s)	59, 272, 450, 1106, 1547, 1756
Lsp1109I	GCAGC	7 cut(s)	1003, 1280, 1520, 1572, 1576, 1949, 1977
LweI	GCATC	5 cut(s)	120, 254, 667, 1112, 1411
MaeI	CTAG	5 cut(s)	191, 851, 1356, 1566, 1842
MaeII	ACGT	2 cut(s)	1169, 2046
MaeIII	GTNAC	3 cut(s)	880, 1732, 2047
MalI	GATC	9 cut(s)	492, 633, 696, 798, 849, 906, 993, 1611, 1793
MboI	GATC	9 cut(s)	490, 631, 694, 796, 847, 904, 991, 1609, 1791
MboII	GAAGA	8 cut(s)	22, 78, 179, 233, 728, 899, 1376, 1463
MflI	RGATCY	5 cut(s)	796, 847, 904, 991, 1791
MhlI	GDGCHC	5 cut(s)	56, 158, 319, 1650, 1782
MlyI	GAGTC	6 cut(s)	113, 178, 206, 581, 826, 1568
Mph1103I	ATGCAT	1 cut(s)	2086
MroXI	GAANNNNTTC	1 cut(s)	309
MslI	CAYNNNNRTG	5 cut(s)	1120, 1142, 1398, 1806, 1973
MspA1I	CMGCKG	1 cut(s)	1301
MspI	CCGG	1 cut(s)	276
MspR9I	CCNGG	9 cut(s)	237, 276, 412, 750, 878, 964, 1127, 1341, 1743
Mva1269I	GAATGC	1 cut(s)	279
MvaI	CCWGG	8 cut(s)	237, 412, 750, 878, 964, 1127, 1341, 1743
MvnI	CGCG	1 cut(s)	585
NciI	CCSGG	1 cut(s)	276
NcoI	CCATGG	1 cut(s)	1543
NdeII	GATC	9 cut(s)	490, 631, 694, 796, 847, 904, 991, 1609, 1791
NlaIII	CATG	9 cut(s)	647, 732, 1141, 1327, 1403, 1421, 1547, 1560, 2084
NlaIV	GGNNCC	5 cut(s)	416, 993, 1394, 1793, 1900
NmuCI	GTSAC	1 cut(s)	2047
NsiI	ATGCAT	1 cut(s)	2086
NspI	RCATGY	2 cut(s)	647, 1141
NspV	TTCGAA	1 cut(s)	215
PaeI	GCATGC	2 cut(s)	647, 1141
PagI	TCATGA	1 cut(s)	1417
PceI	AGGCCT	1 cut(s)	1746
PciSI	GCTCTTC	1 cut(s)	1359
PctI	GAATGC	1 cut(s)	279
PdmI	GAANNNNTTC	1 cut(s)	309
PfeI	GAWTC	7 cut(s)	212, 224, 244, 554, 745, 1412, 1694
PflMI	CCANNNNNTGG	1 cut(s)	1340
PkrI	GCNGC	9 cut(s)	20, 759, 1018, 1270, 1510, 1562, 1591, 1964, 1967
PleI	GAGTC	6 cut(s)	113, 178, 205, 581, 826, 1568
PpsI	GAGTC	6 cut(s)	113, 178, 205, 581, 826, 1568
Psp124BI	GAGCTC	2 cut(s)	56, 1782
Psp6I	CCWGG	8 cut(s)	235, 410, 748, 876, 962, 1125, 1339, 1741
PspGI	CCWGG	8 cut(s)	235, 410, 748, 876, 962, 1125, 1339, 1741
PspN4I	GGNNCC	5 cut(s)	416, 993, 1394, 1793, 1900
PspPI	GGNCC	3 cut(s)	1553, 1717, 1933
PstI	CTGCAG	3 cut(s)	1021, 1510, 1967
PstNI	CAGNNNCTG	1 cut(s)	557
PsuI	RGATCY	5 cut(s)	796, 847, 904, 991, 1791
RsaI	GTAC	2 cut(s)	405, 1114
RsaNI	GTAC	2 cut(s)	404, 1113
RseI	CAYNNNNRTG	5 cut(s)	1120, 1142, 1398, 1806, 1973
SacI	GAGCTC	2 cut(s)	56, 1782
SalI	GTCGAC	1 cut(s)	59
SapI	GCTCTTC	1 cut(s)	1359
SatI	GCNGC	9 cut(s)	19, 758, 1017, 1269, 1509, 1561, 1590, 1963, 1966
Sau3AI	GATC	9 cut(s)	490, 631, 694, 796, 847, 904, 991, 1609, 1791
Sau96I	GGNCC	3 cut(s)	1553, 1717, 1933
SchI	GAGTC	6 cut(s)	113, 178, 206, 581, 826, 1568
ScrFI	CCNGG	9 cut(s)	237, 276, 412, 750, 878, 964, 1127, 1341, 1743
SduI	GDGCHC	5 cut(s)	56, 158, 319, 1650, 1782
SfaNI	GCATC	5 cut(s)	120, 254, 667, 1112, 1411
SfcI	CTRYAG	6 cut(s)	600, 823, 1017, 1263, 1506, 1963
SfuI	TTCGAA	1 cut(s)	215
SgrDI	CGTCGACG	1 cut(s)	59
SinI	GGWCC	2 cut(s)	1553, 1717
SmiMI	CAYNNNNRTG	5 cut(s)	1120, 1142, 1398, 1806, 1973
SmlI	CTYRAG	6 cut(s)	589, 787, 800, 1088, 1207, 1283
SmoI	CTYRAG	6 cut(s)	589, 787, 800, 1088, 1207, 1283
SphI	GCATGC	2 cut(s)	647, 1141
SseBI	AGGCCT	1 cut(s)	1746
SsiI	CCGC	4 cut(s)	18, 758, 1299, 1708
SspI	AATATT	1 cut(s)	1293
SspMI	CTAG	5 cut(s)	191, 851, 1356, 1566, 1842
SstI	GAGCTC	2 cut(s)	56, 1782
StuI	AGGCCT	1 cut(s)	1746
StyD4I	CCNGG	9 cut(s)	235, 274, 410, 748, 876, 962, 1125, 1339, 1741
StyI	CCWWGG	5 cut(s)	892, 1102, 1543, 1713, 1903
TaaI	ACNGT	9 cut(s)	45, 153, 301, 886, 1238, 1280, 1732, 1911, 1949
TaiI	ACGT	2 cut(s)	1172, 2049
TaqI	TCGA	5 cut(s)	60, 114, 215, 227, 1987
TatI	WGTACW	1 cut(s)	403
TauI	GCSGC	2 cut(s)	21, 760
TfiI	GAWTC	7 cut(s)	212, 224, 244, 554, 745, 1412, 1694
TscAI	CASTG	5 cut(s)	142, 933, 1632, 1735, 1864
TseFI	GTSAC	1 cut(s)	2047
TseI	GCWGC	7 cut(s)	1016, 1268, 1508, 1560, 1589, 1962, 1965
Tsp45I	GTSAC	1 cut(s)	2047
TspDTI	ATGAA	4 cut(s)	234, 446, 899, 2084
TspGWI	ACGGA	4 cut(s)	46, 259, 1455, 1883
TspRI	CASTG	5 cut(s)	142, 933, 1632, 1735, 1864
Van91I	CCANNNNNTGG	1 cut(s)	1340
VneI	GTGCAC	1 cut(s)	315
VpaK11BI	GGWCC	2 cut(s)	1553, 1717
XapI	RAATTY	5 cut(s)	637, 1027, 1253, 2003, 2139
XceI	RCATGY	2 cut(s)	647, 1141
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1711, 1892
XmiI	GTMKAC	2 cut(s)	60, 1223
XmnI	GAANNNNTTC	1 cut(s)	309
XspI	CTAG	5 cut(s)	191, 851, 1356, 1566, 1842
Zsp2I	ATGCAT	1 cut(s)	2086

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.