AT5G46220

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011446
Physical Location & Seq
Forward (+)
18738697 .. 18742126
3430 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46220.1

Sequence Viewer

Length: 1389 bp
ATGGGAAAATTCATCACAACCACACTATCTCCACCGCTCTATGCCCGATCAAAGCTTCTCTGTTTTTCACTTCTCTACTTATTCAGTACAATCTTTCTGTTCCTCTACGTATCTCTCTCAAGAAACCAATGCATCTTTCGATATTCGCCATTTGATCCGATTCAAGCAAAACTTTTCTCTTATCCTTCTTCTTATGGTGAACATAAATATGCTCTTCCCACTCATCGATCTTCTTGTTCTTCCCCTATTTTCTTTTCAGATTATTGGACAGTGTTGAAAGAGATACAGAGTATCTTGAGCGGTTCATCACCAAAGGAAAATTTGAGATATATCAATGGAAAAAGTGAAAGTTTCGGTGGAAATTTCAGTACTCAGAAGAGATTTTCTTATTTCAATCATTCGAACATTGACGTCGAAGTTCCATGCGGCTTCTTCAGAGATTTTCCGGTCAGCAATTCCGATAGAGTTGAGATGGAGAAATGTGGACTAGTAGTTGCATCAGCCATTTTTAACGACCATGACAAGATAAGACAACCAGTGGGACTTGGAGTTAAGACCTTAGAAACCGTTTGCTTCTACATGTTCATCGACGATAAAACCTTAAATTCTCTCTTCCACCACAATGTCATCCTGAAAAACAATCCAAGTGATTATAGAGTTGGTGCATGGAGAATCATCAAAATTTCGAAATCCGAAAATCTATACCTTAATCCGGCTATGAACGGGGTTATACCAAAGTATCTAATTCATAGATTGTTTCCAAACTCAAAGTTCAGTATATGGGTCGATGCGAAAATTCAGCTCATGATTGATCCGCTACTTCTAATTCATTCAATGTTAGTAGTGCCTGAAGTCGATATGGCGATATCGAAACATCCGTTTTTTGTTAATACAATGGAGGAAGCTATGGCTACCGCGCGGTGGAAGAAATGGGGCGATGTCGACGGGCTAAGGATTCAAATGGAGACTTATTGTGAGCATGGTTTGAAGCCTTGGAGCTCTAGTAAGTTGCCATATCCCACAGATGTACCAGATACCGCGTTGATACTGCGAAGACACGGAATCAGAAGCAACCTATTCTCGTGTTTCATGTTCAACGAGTTAGAAGCGTTTAACCCGCGAGACCAATTGGCGTTTGCGTTTGTGAGGGATCACATAAACCCGAAGGTGAAAATGAACATGTTTGAAGTCGAAGTGTTCGAGCAAGTAGTTGTTGAATACAGACACAATCTCAAGAAGATTGAGAGCTCTACGTATGAGGAACAAGAAGAAGAACAGAAACAAGAATCGTTGAGGACAATACAAAAGAGAAGAAAATGGCTAGATCATGAATCTTGGTCTCTCAATCGTAGTAGCTGTAAGAATTATCTCACGGATATGTGGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

462

Amino Acids

53.88

Weight (kDa)

8.66

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 114 - 409 1.1e-112 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46220
fragaria_vesca FvH4_3g05570 FvH4_3g05570
malus_domestica MD05G1284000.v1.1 MD10G1262300.v1.1
prunus_persica Prupe.4G080400_v2.0.a1 Prupe.4G080400_v2.0.a1
pyrus_communis pycom05g26310 pycom10g21850
rosa_chinensis RchiOBHm_Chr5g0014101
rosa_laevigata RLG00000032081
rosa_multiflora Rmu_sc0007767.1_g000016
rosa_roxburghii Rroxscaffold_1G00062530
rosa_rugosa Rorug05G0015700
rosa_samantha Rh5AG109600 Rh5BG106400 Rh5BG106500 Rh5CG118300 Rh5DG105100
rosa_wichuraiana Rw5G009570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 414
AccBSI CCGCTC 2 cut(s) 37, 300
AccI GTMKAC 1 cut(s) 942
AccII CGCG 4 cut(s) 917, 919, 1040, 1120
AciI CCGC 8 cut(s) 35, 300, 426, 815, 915, 919, 1038, 1118
AclWI GGATC 3 cut(s) 149, 806, 1158
AcsI RAATTY 6 cut(s) 8, 319, 361, 604, 681, 795
AcuI CTGAAG 2 cut(s) 418, 870
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 3 cut(s) 88, 370, 1029
AfiI CCNNNNNNNGG 2 cut(s) 712, 921
AflIII ACRYGT 2 cut(s) 579, 1179
AgsI TTSAA 9 cut(s) 164, 277, 394, 834, 959, 988, 1096, 1187, 1217
AhlI ACTAGT 1 cut(s) 487
AjuI GAANNNNNNNTTGG 2 cut(s) 120, 152
AluBI AGCT 6 cut(s) 55, 802, 905, 999, 1248, 1356
AluI AGCT 6 cut(s) 55, 802, 905, 999, 1248, 1356
Alw21I GWGCWC 2 cut(s) 1001, 1250
Alw26I GTCTC 3 cut(s) 959, 1116, 1344
AlwI GGATC 3 cut(s) 149, 806, 1158
ApoI RAATTY 6 cut(s) 8, 319, 361, 604, 681, 795
AspLEI GCGC 1 cut(s) 919
AsuHPI GGTGA 3 cut(s) 209, 300, 1180
AsuII TTCGAA 2 cut(s) 401, 686
BaeI ACNNNNGTAYC 4 cut(s) 722, 755, 1011, 1044
BanII GRGCYC 2 cut(s) 1001, 1250
BauI CACGAG 1 cut(s) 1081
BbsI GAAGAC 1 cut(s) 1060
Bbv12I GWGCWC 2 cut(s) 1001, 1250
BccI CCATC 1 cut(s) 466
BcoDI GTCTC 3 cut(s) 959, 1116, 1344
BcuI ACTAGT 1 cut(s) 487
BfaI CTAG 3 cut(s) 488, 1002, 1322
BisI GCNGC 1 cut(s) 427
BlsI GCNGC 1 cut(s) 428
BmcAI AGTACT 1 cut(s) 370
BmsI GCATC 3 cut(s) 141, 506, 778
BpiI GAAGAC 1 cut(s) 1060
Bpu10I CCTNAGC 1 cut(s) 950
Bpu14I TTCGAA 2 cut(s) 401, 686
BpuEI CTTGAG 3 cut(s) 103, 316, 1217
Bsa29I ATCGAT 1 cut(s) 226
BsaAI YACGTR 2 cut(s) 109, 1254
BsaHI GRCGYC 1 cut(s) 411
BsaI GGTCTC 2 cut(s) 1116, 1344
BsaJI CCNNGG 1 cut(s) 992
BsaWI WCCGGW 1 cut(s) 445
BsaXI ACNNNNNCTCC 4 cut(s) 13, 43, 988, 1018
Bsc4I CCNNNNNNNGG 2 cut(s) 712, 921
Bse1I ACTGG 1 cut(s) 536
BseCI ATCGAT 1 cut(s) 226
BseDI CCNNGG 1 cut(s) 992
BseGI GGATG 2 cut(s) 627, 874
BseLI CCNNNNNNNGG 2 cut(s) 712, 921
BseMII CTCAG 1 cut(s) 386
BseNI ACTGG 1 cut(s) 536
Bsh1236I CGCG 4 cut(s) 917, 919, 1040, 1120
BshVI ATCGAT 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 1001, 1250
BsiSI CCGG 2 cut(s) 446, 713
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 2 cut(s) 712, 921
BsmAI GTCTC 3 cut(s) 959, 1116, 1344
BsmFI GGGAC 1 cut(s) 555
Bso31I GGTCTC 2 cut(s) 1116, 1344
Bsp119I TTCGAA 2 cut(s) 401, 686
Bsp1286I GDGCHC 2 cut(s) 1001, 1250
Bsp143I GATC 6 cut(s) 47, 154, 227, 811, 1150, 1324
BspACI CCGC 8 cut(s) 35, 300, 426, 815, 915, 919, 1038, 1118
BspCNI CTCAG 1 cut(s) 385
BspDI ATCGAT 1 cut(s) 226
BspFNI CGCG 4 cut(s) 917, 919, 1040, 1120
BspHI TCATGA 2 cut(s) 804, 1327
BspPI GGATC 3 cut(s) 149, 806, 1158
BspQI GCTCTTC 1 cut(s) 219
BspT104I TTCGAA 2 cut(s) 401, 686
BspTNI GGTCTC 2 cut(s) 1116, 1344
BsrBI CCGCTC 2 cut(s) 37, 300
BsrI ACTGG 1 cut(s) 536
BssECI CCNNGG 1 cut(s) 992
BssMI GATC 6 cut(s) 47, 154, 227, 811, 1150, 1324
BssNI GRCGYC 1 cut(s) 411
BssSI CACGAG 1 cut(s) 1081
BssT1I CCWWGG 1 cut(s) 992
Bst2BI CACGAG 1 cut(s) 1081
Bst4CI ACNGT 2 cut(s) 271, 568
Bst6I CTCTTC 3 cut(s) 219, 371, 617
BstACI GRCGYC 1 cut(s) 411
BstBAI YACGTR 2 cut(s) 109, 1254
BstBI TTCGAA 2 cut(s) 401, 686
BstDEI CTNAG 3 cut(s) 372, 559, 950
BstF5I GGATG 2 cut(s) 627, 874
BstFNI CGCG 4 cut(s) 917, 919, 1040, 1120
BstHHI GCGC 1 cut(s) 919
BstKTI GATC 6 cut(s) 50, 157, 230, 814, 1153, 1327
BstMAI GTCTC 3 cut(s) 959, 1116, 1344
BstMBI GATC 6 cut(s) 47, 154, 227, 811, 1150, 1324
BstNSI RCATGY 2 cut(s) 583, 1183
BstSNI TACGTA 2 cut(s) 109, 1254
BstUI CGCG 4 cut(s) 917, 919, 1040, 1120
BstV2I GAAGAC 1 cut(s) 1060
Bsu15I ATCGAT 1 cut(s) 226
BsuTUI ATCGAT 1 cut(s) 226
BtgZI GCGATG 1 cut(s) 951
BtsCI GGATG 2 cut(s) 627, 874
BtsIMutI CAGTG 2 cut(s) 276, 543
CciI TCATGA 2 cut(s) 804, 1327
CfoI GCGC 1 cut(s) 919
ClaI ATCGAT 1 cut(s) 226
Csp6I GTAC 3 cut(s) 87, 369, 1028
CviAII CATG 9 cut(s) 423, 518, 580, 666, 805, 980, 1090, 1180, 1328
CviQI GTAC 3 cut(s) 87, 369, 1028
DdeI CTNAG 3 cut(s) 372, 559, 950
DpnI GATC 6 cut(s) 49, 156, 229, 813, 1152, 1326
DpnII GATC 6 cut(s) 47, 154, 227, 811, 1150, 1324
Eam1104I CTCTTC 3 cut(s) 219, 371, 617
EarI CTCTTC 3 cut(s) 219, 371, 617
Ecl136II GAGCTC 2 cut(s) 999, 1248
Eco105I TACGTA 2 cut(s) 109, 1254
Eco130I CCWWGG 1 cut(s) 992
Eco24I GRGCYC 2 cut(s) 1001, 1250
Eco31I GGTCTC 2 cut(s) 1116, 1344
Eco32I GATATC 1 cut(s) 867
Eco53kI GAGCTC 2 cut(s) 999, 1248
Eco57I CTGAAG 2 cut(s) 418, 870
EcoICRI GAGCTC 2 cut(s) 999, 1248
EcoRV GATATC 1 cut(s) 867
EcoT14I CCWWGG 1 cut(s) 992
EcoT22I ATGCAT 1 cut(s) 134
EcoT38I GRGCYC 2 cut(s) 1001, 1250
ErhI CCWWGG 1 cut(s) 992
FaeI CATG 9 cut(s) 426, 521, 583, 669, 808, 983, 1093, 1183, 1331
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FaqI GGGAC 1 cut(s) 555
FatI CATG 9 cut(s) 422, 517, 579, 665, 804, 979, 1089, 1179, 1327
FauI CCCGC 1 cut(s) 1125
FblI GTMKAC 1 cut(s) 942
Fnu4HI GCNGC 1 cut(s) 427
FokI GGATG 2 cut(s) 614, 861
FriOI GRGCYC 2 cut(s) 1001, 1250
Fsp4HI GCNGC 1 cut(s) 427
FspBI CTAG 3 cut(s) 488, 1002, 1322
GlaI GCGC 1 cut(s) 918
GluI GCNGC 1 cut(s) 427
HapII CCGG 2 cut(s) 446, 713
HhaI GCGC 1 cut(s) 919
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 9 cut(s) 426, 521, 583, 669, 808, 983, 1093, 1183, 1331
Hin6I GCGC 1 cut(s) 917
HinP1I GCGC 1 cut(s) 917
HincII GTYRAC 1 cut(s) 943
HindII GTYRAC 1 cut(s) 943
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 6 cut(s) 160, 672, 955, 1062, 1286, 1331
HpaII CCGG 2 cut(s) 446, 713
HphI GGTGA 3 cut(s) 209, 300, 1180
Hpy166II GTNNAC 3 cut(s) 200, 485, 943
Hpy188I TCNGA 7 cut(s) 159, 259, 375, 437, 460, 694, 1067
Hpy188III TCNNGA 6 cut(s) 120, 295, 631, 805, 1234, 1328
Hpy8I GTNNAC 3 cut(s) 200, 485, 943
Hpy99I CGWCG 3 cut(s) 416, 593, 947
HpyAV CCTTC 2 cut(s) 195, 1159
HpyCH4III ACNGT 2 cut(s) 271, 568
HpyCH4IV ACGT 3 cut(s) 108, 411, 1253
HpyCH4V TGCA 3 cut(s) 132, 497, 665
HpyF3I CTNAG 3 cut(s) 372, 559, 950
HpySE526I ACGT 3 cut(s) 108, 411, 1253
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 9 cut(s) 426, 521, 583, 669, 808, 983, 1093, 1183, 1331
HspAI GCGC 1 cut(s) 917
Kzo9I GATC 6 cut(s) 47, 154, 227, 811, 1150, 1324
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 1 cut(s) 996
LpnPI CCDG 6 cut(s) 459, 549, 644, 726, 861, 1044
LweI GCATC 3 cut(s) 141, 506, 778
MaeI CTAG 3 cut(s) 488, 1002, 1322
MaeII ACGT 3 cut(s) 108, 411, 1253
MalI GATC 6 cut(s) 49, 156, 229, 813, 1152, 1326
MbiI CCGCTC 2 cut(s) 37, 300
MboI GATC 6 cut(s) 47, 154, 227, 811, 1150, 1324
MfeI CAATTG 1 cut(s) 1127
MhlI GDGCHC 2 cut(s) 1001, 1250
MnlI CCTC 5 cut(s) 113, 892, 1140, 1252, 1287
Mph1103I ATGCAT 1 cut(s) 134
MseI TTAA 6 cut(s) 510, 552, 602, 708, 888, 1113
MslI CAYNNNNRTG 3 cut(s) 207, 621, 1376
MspI CCGG 2 cut(s) 446, 713
MunI CAATTG 1 cut(s) 1127
MvnI CGCG 4 cut(s) 917, 919, 1040, 1120
NdeII GATC 6 cut(s) 47, 154, 227, 811, 1150, 1324
NlaIII CATG 9 cut(s) 426, 521, 583, 669, 808, 983, 1093, 1183, 1331
NsiI ATGCAT 1 cut(s) 134
NspI RCATGY 2 cut(s) 583, 1183
NspV TTCGAA 2 cut(s) 401, 686
PagI TCATGA 2 cut(s) 804, 1327
PciI ACATGT 2 cut(s) 579, 1179
PciSI GCTCTTC 1 cut(s) 219
PcsI WCGNNNNNNNCGW 2 cut(s) 875, 1197
PfeI GAWTC 6 cut(s) 160, 672, 955, 1062, 1286, 1331
PkrI GCNGC 1 cut(s) 428
Ppu21I YACGTR 2 cut(s) 109, 1254
PscI ACATGT 2 cut(s) 579, 1179
Psp124BI GAGCTC 2 cut(s) 1001, 1250
RsaI GTAC 3 cut(s) 88, 370, 1029
RsaNI GTAC 3 cut(s) 87, 369, 1028
RseI CAYNNNNRTG 3 cut(s) 207, 621, 1376
SacI GAGCTC 2 cut(s) 1001, 1250
SalI GTCGAC 1 cut(s) 941
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 6 cut(s) 510, 552, 602, 708, 888, 1113
SatI GCNGC 1 cut(s) 427
Sau3AI GATC 6 cut(s) 47, 154, 227, 811, 1150, 1324
ScaI AGTACT 1 cut(s) 370
SduI GDGCHC 2 cut(s) 1001, 1250
SfaNI GCATC 3 cut(s) 141, 506, 778
SfuI TTCGAA 2 cut(s) 401, 686
SmiMI CAYNNNNRTG 3 cut(s) 207, 621, 1376
SmlI CTYRAG 3 cut(s) 118, 295, 1232
SmoI CTYRAG 3 cut(s) 118, 295, 1232
SnaBI TACGTA 2 cut(s) 109, 1254
SpeI ACTAGT 1 cut(s) 487
SsiI CCGC 8 cut(s) 35, 300, 426, 815, 915, 919, 1038, 1118
SspMI CTAG 3 cut(s) 488, 1002, 1322
SstI GAGCTC 2 cut(s) 1001, 1250
StyI CCWWGG 1 cut(s) 992
TaaI ACNGT 2 cut(s) 271, 568
TaiI ACGT 3 cut(s) 111, 414, 1256
TatI WGTACW 2 cut(s) 86, 368
TauI GCSGC 1 cut(s) 429
TfiI GAWTC 6 cut(s) 160, 672, 955, 1062, 1286, 1331
Tru1I TTAA 6 cut(s) 510, 552, 602, 708, 888, 1113
Tru9I TTAA 6 cut(s) 510, 552, 602, 708, 888, 1113
TscAI CASTG 2 cut(s) 276, 543
TspDTI ATGAA 8 cut(s) 294, 574, 734, 737, 818, 1078, 1190, 1344
TspGWI ACGGA 3 cut(s) 867, 1074, 1388
TspRI CASTG 2 cut(s) 276, 543
XapI RAATTY 6 cut(s) 8, 319, 361, 604, 681, 795
XceI RCATGY 2 cut(s) 583, 1183
XmiI GTMKAC 1 cut(s) 942
XspI CTAG 3 cut(s) 488, 1002, 1322
ZraI GACGTC 1 cut(s) 412
ZrmI AGTACT 1 cut(s) 370
Zsp2I ATGCAT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.