Rh5BG106400

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011446
Physical Location & Seq
Reverse (-)
9746348 .. 9749273
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG106400.1

Sequence Viewer

Length: 1344 bp
ATGGGGAAAGTAACGTCCACTCCTCTGCTCTTTCAATCAAAGCTGGTCTGTTTCTCTCTGCTCTACCTCTTCACCTCTCTGTTTCTAGCTCTCTACACCTCTCTCTCCCAGACCAAATGCCTCTTCAGATCTTCACCTTTCGATCCAATTCAATCCCCTCTCTTCTCCTACCCTCCCAATTATGGAGAACACAAGTATGCAATCCCAACCCATCGTAAATCTTGCTCTTCGCCTGTCCATTTCTCAGATTATTGGAAGGTCTTGAAGGAGATCCAAAATTTGTGCAAGAATTCCTCTTGGAATTCATCGGTTTTGAGATATATGCAGGGAACGTCTGAGACTTTTGGAGGGAATTTCAGTACCCAAAATAGGATTTCCTATTTTGTTCATTACAATGAGAGCACCGAAATCCCTTGTGGGTTCCTCAAGAAATTTCCAGTTAGTGATTCTGATAGAATTGTCATGGAAGCGTGTAAAGGACTTGTAGTTGTCTCGGCTATCTTCAATGATCATGATAAAATTCGACAACCCAAGGGGATTGGATCAAAAACCCTTGAGAATGTATGTTTCTTTATGTTTATAGACGAGACAACCCTCAAAGGATTTTACCAGCATCATTTGATTCCAGAGAAATCTGAAAACTATAAGATTGGTGCATGGAGGGTTGTCAAGGTTTCGAGCAATGGATTGTATGAGAATCCGGCAATGAATGGTGTGATACCAAAGTACTTGGTTCATAGACTTTTCCCAAACGCCAAATTCAGCATTTGGGTGGATGCGAAAATGCAGCTGATGGTTGATCCATTACTTTTGATTCATACACTTGTTGTGAATGAGAACGCCGACATGGCGATTTCAAAGCATCCTTACTATGTTCATACTATGGAAGAAGCAATGGCGACGGCGAGGTGGAAGAAATGGAGGGACGTTGATGGATTGAAGAGGCAAATGGAGACTTATTGTGAGAATGGCTTGCTCCCCTGGACTCCTGATAAGCTTCCTTATCCCTCAGATGTACCAGATAGTGCTTTGATTCTTAGGAAACATGGACTGAGGAGCAATCTATTCTCCTGCCTATTGTTTAATGAGATGGAGGCATTCAACCCAAGAGACCAATTAGCCTTTGCATATGTGAGAGACCTCATGAGCCCAAGGCTGAAGCTGAACATGTTTTCAGTTGAAGTGTTTGAGCAAATTGCTGTGGAATACAGGCACAACCTTAAGCCTGGAGTTGGGACCAGTGGAGGTGAATCTAAGGGGGGACCCAAAACGAAAAAGACCCAAAGGGCAGGCCATGATTTGTTGTATGTCAATGGCAGCTGTTGCAGTACGTGTCAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

447

Amino Acids

51.08

Weight (kDa)

9.01

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 111 - 405 6.9e-112 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46220
fragaria_vesca FvH4_3g05570 FvH4_3g05570
malus_domestica MD05G1284000.v1.1 MD10G1262300.v1.1
prunus_persica Prupe.4G080400_v2.0.a1 Prupe.4G080400_v2.0.a1
pyrus_communis pycom05g26310 pycom10g21850
rosa_chinensis RchiOBHm_Chr5g0014101
rosa_laevigata RLG00000032081
rosa_multiflora Rmu_sc0007767.1_g000016
rosa_roxburghii Rroxscaffold_1G00062530
rosa_rugosa Rorug05G0015700
rosa_samantha Rh5AG109600 Rh5BG106400 Rh5BG106500 Rh5CG118300 Rh5DG105100
rosa_wichuraiana Rw5G009570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 137, 265, 550, 794
AcsI RAATTY 7 cut(s) 277, 289, 301, 352, 431, 519, 758
AcuI CTGAAG 2 cut(s) 109, 1178
AfaI GTAC 4 cut(s) 361, 728, 1017, 1330
AfiI CCNNNNNNNGG 3 cut(s) 182, 369, 1232
AflII CTTAAG 1 cut(s) 1220
AflIII ACRYGT 2 cut(s) 1167, 1331
AgsI TTSAA 8 cut(s) 35, 152, 265, 505, 858, 940, 1102, 1181
AjnI CCWGG 2 cut(s) 980, 1225
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 6 cut(s) 43, 89, 790, 997, 1162, 1320
AluI AGCT 6 cut(s) 43, 89, 790, 997, 1162, 1320
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 6 cut(s) 332, 496, 581, 947, 1104, 1131
AlwI GGATC 4 cut(s) 137, 265, 550, 794
AoxI GGCC 1 cut(s) 1291
ApeKI GCWGC 2 cut(s) 787, 1317
ApoI RAATTY 7 cut(s) 277, 289, 301, 352, 431, 519, 758
AspS9I GGNCC 2 cut(s) 1236, 1262
AsuHPI GGTGA 3 cut(s) 64, 126, 1259
AvaII GGWCC 2 cut(s) 1236, 1262
BaeI ACNNNNGTAYC 2 cut(s) 710, 743
BanII GRGCYC 1 cut(s) 1151
Bbv12I GWGCWC 1 cut(s) 404
BbvI GCAGC 2 cut(s) 799, 1329
BccI CCATC 4 cut(s) 219, 787, 926, 1084
BceAI ACGGC 1 cut(s) 918
BciT130I CCWGG 2 cut(s) 982, 1227
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 6 cut(s) 332, 496, 581, 947, 1104, 1131
BfaI CTAG 1 cut(s) 86
BfrI CTTAAG 1 cut(s) 1220
BglI GCCNNNNNGGC 1 cut(s) 848
BglII AGATCT 1 cut(s) 128
BisI GCNGC 2 cut(s) 788, 1318
BlsI GCNGC 2 cut(s) 789, 1319
BmcAI AGTACT 1 cut(s) 728
Bme1390I CCNGG 2 cut(s) 982, 1227
Bme18I GGWCC 2 cut(s) 1236, 1262
BmgT120I GGNCC 2 cut(s) 1236, 1262
BmiI GGNNCC 4 cut(s) 422, 1237, 1263, 1264
BmrFI CCNGG 2 cut(s) 982, 1227
BmsI GCATC 3 cut(s) 622, 766, 871
BpmI CTGGAG 1 cut(s) 1248
BpuEI CTTGAG 2 cut(s) 410, 575
BsaAI YACGTR 1 cut(s) 1332
BsaI GGTCTC 2 cut(s) 1104, 1131
BsaJI CCNNGG 3 cut(s) 531, 980, 1151
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 369, 1232
Bse1I ACTGG 2 cut(s) 437, 1239
Bse3DI GCAATG 3 cut(s) 688, 711, 900
BseBI CCWGG 2 cut(s) 982, 1227
BseDI CCNNGG 3 cut(s) 531, 980, 1151
BseGI GGATG 2 cut(s) 781, 862
BseLI CCNNNNNNNGG 3 cut(s) 182, 369, 1232
BseMI GCAATG 3 cut(s) 688, 711, 900
BseMII CTCAG 4 cut(s) 258, 327, 1023, 1043
BseNI ACTGG 2 cut(s) 437, 1239
BseRI GAGGAG 2 cut(s) 12, 1069
BseXI GCAGC 2 cut(s) 799, 1329
BshFI GGCC 1 cut(s) 1293
BsiHKAI GWGCWC 1 cut(s) 404
BsiSI CCGG 1 cut(s) 701
BslFI GGGAC 3 cut(s) 938, 1249, 1275
BslI CCNNNNNNNGG 3 cut(s) 182, 369, 1232
BsmAI GTCTC 6 cut(s) 332, 496, 581, 947, 1104, 1131
BsmFI GGGAC 3 cut(s) 938, 1249, 1275
BsmI GAATGC 1 cut(s) 1097
BsnI GGCC 1 cut(s) 1293
Bso31I GGTCTC 2 cut(s) 1104, 1131
Bsp1286I GDGCHC 2 cut(s) 404, 1151
Bsp143I GATC 6 cut(s) 128, 142, 270, 508, 542, 799
BspANI GGCC 1 cut(s) 1293
BspCNI CTCAG 4 cut(s) 257, 328, 1022, 1044
BspHI TCATGA 2 cut(s) 511, 1143
BspLI GGNNCC 4 cut(s) 422, 1237, 1263, 1264
BspPI GGATC 4 cut(s) 137, 265, 550, 794
BspQI GCTCTTC 1 cut(s) 232
BspTI CTTAAG 1 cut(s) 1220
BspTNI GGTCTC 2 cut(s) 1104, 1131
BsrDI GCAATG 3 cut(s) 688, 711, 900
BsrI ACTGG 2 cut(s) 437, 1239
BssECI CCNNGG 3 cut(s) 531, 980, 1151
BssMI GATC 6 cut(s) 128, 142, 270, 508, 542, 799
BssT1I CCWWGG 2 cut(s) 531, 1151
Bst2UI CCWGG 2 cut(s) 982, 1227
Bst6I CTCTTC 5 cut(s) 74, 128, 167, 232, 935
BstAFI CTTAAG 1 cut(s) 1220
BstAPI GCANNNNNTGC 1 cut(s) 1323
BstBAI YACGTR 1 cut(s) 1332
BstC8I GCNNGC 2 cut(s) 974, 1291
BstDEI CTNAG 6 cut(s) 244, 336, 1009, 1037, 1052, 1254
BstF5I GGATG 2 cut(s) 781, 862
BstKTI GATC 6 cut(s) 131, 145, 273, 511, 545, 802
BstMAI GTCTC 6 cut(s) 332, 496, 581, 947, 1104, 1131
BstMBI GATC 6 cut(s) 128, 142, 270, 508, 542, 799
BstMWI GCNNNNNNNGC 2 cut(s) 848, 1323
BstNI CCWGG 2 cut(s) 982, 1227
BstNSI RCATGY 1 cut(s) 1171
BstSCI CCNGG 2 cut(s) 980, 1225
BstV1I GCAGC 2 cut(s) 799, 1329
BstX2I RGATCY 2 cut(s) 128, 270
BstYI RGATCY 2 cut(s) 128, 270
BsuRI GGCC 1 cut(s) 1293
BtsCI GGATG 2 cut(s) 781, 862
BtsIMutI CAGTG 1 cut(s) 1246
Cac8I GCNNGC 2 cut(s) 974, 1291
CciI TCATGA 2 cut(s) 511, 1143
Cfr13I GGNCC 2 cut(s) 1236, 1262
Csp6I GTAC 4 cut(s) 360, 727, 1016, 1329
CviAII CATG 8 cut(s) 463, 512, 657, 847, 1046, 1144, 1168, 1295
CviQI GTAC 4 cut(s) 360, 727, 1016, 1329
DdeI CTNAG 6 cut(s) 244, 336, 1009, 1037, 1052, 1254
DpnI GATC 6 cut(s) 130, 144, 272, 510, 544, 801
DpnII GATC 6 cut(s) 128, 142, 270, 508, 542, 799
Eam1104I CTCTTC 5 cut(s) 74, 128, 167, 232, 935
EarI CTCTTC 5 cut(s) 74, 128, 167, 232, 935
Eco130I CCWWGG 2 cut(s) 531, 1151
Eco24I GRGCYC 1 cut(s) 1151
Eco31I GGTCTC 2 cut(s) 1104, 1131
Eco47I GGWCC 2 cut(s) 1236, 1262
Eco57I CTGAAG 2 cut(s) 109, 1178
EcoO109I RGGNCCY 1 cut(s) 1262
EcoRI GAATTC 2 cut(s) 289, 301
EcoRII CCWGG 2 cut(s) 980, 1225
EcoT14I CCWWGG 2 cut(s) 531, 1151
EcoT38I GRGCYC 1 cut(s) 1151
ErhI CCWWGG 2 cut(s) 531, 1151
FaeI CATG 8 cut(s) 466, 515, 660, 850, 1049, 1147, 1171, 1298
FaqI GGGAC 3 cut(s) 938, 1249, 1275
FatI CATG 8 cut(s) 462, 511, 656, 846, 1045, 1143, 1167, 1294
FauNDI CATATG 1 cut(s) 1129
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 2 cut(s) 788, 1318
FokI GGATG 2 cut(s) 788, 849
FriOI GRGCYC 1 cut(s) 1151
Fsp4HI GCNGC 2 cut(s) 788, 1318
FspBI CTAG 1 cut(s) 86
GluI GCNGC 2 cut(s) 788, 1318
GsuI CTGGAG 1 cut(s) 1248
HaeIII GGCC 1 cut(s) 1293
HapII CCGG 1 cut(s) 701
Hin1II CATG 8 cut(s) 466, 515, 660, 850, 1049, 1147, 1171, 1298
HindIII AAGCTT 1 cut(s) 995
HinfI GANTC 7 cut(s) 446, 622, 697, 814, 985, 1033, 1250
HpaII CCGG 1 cut(s) 701
HphI GGTGA 3 cut(s) 64, 126, 1259
Hpy166II GTNNAC 1 cut(s) 18
Hpy188I TCNGA 7 cut(s) 128, 247, 337, 451, 637, 1012, 1338
Hpy188III TCNNGA 6 cut(s) 262, 427, 512, 626, 989, 1144
Hpy8I GTNNAC 1 cut(s) 18
Hpy99I CGWCG 1 cut(s) 904
HpyAV CCTTC 2 cut(s) 250, 259
HpyCH4IV ACGT 4 cut(s) 14, 332, 927, 1331
HpyCH4V TGCA 7 cut(s) 200, 285, 325, 656, 787, 1127, 1326
HpyF10VI GCNNNNNNNGC 2 cut(s) 848, 1323
HpyF3I CTNAG 6 cut(s) 244, 336, 1009, 1037, 1052, 1254
HpySE526I ACGT 4 cut(s) 14, 332, 927, 1331
Hsp92II CATG 8 cut(s) 466, 515, 660, 850, 1049, 1147, 1171, 1298
KflI GGGWCCC 1 cut(s) 1262
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 6 cut(s) 128, 142, 270, 508, 542, 799
LguI GCTCTTC 1 cut(s) 232
LmnI GCTCC 2 cut(s) 981, 1056
Lsp1109I GCAGC 2 cut(s) 799, 1329
LweI GCATC 3 cut(s) 622, 766, 871
MaeI CTAG 1 cut(s) 86
MaeII ACGT 4 cut(s) 14, 332, 927, 1331
MaeIII GTNAC 1 cut(s) 10
MalI GATC 6 cut(s) 130, 144, 272, 510, 544, 801
MboI GATC 6 cut(s) 128, 142, 270, 508, 542, 799
MboII GAAGA 9 cut(s) 61, 115, 123, 154, 219, 493, 899, 925, 952
MflI RGATCY 2 cut(s) 128, 270
MhlI GDGCHC 2 cut(s) 404, 1151
MlyI GAGTC 1 cut(s) 979
MseI TTAA 2 cut(s) 1083, 1221
MslI CAYNNNNRTG 3 cut(s) 195, 393, 770
MspA1I CMGCKG 2 cut(s) 790, 1320
MspCI CTTAAG 1 cut(s) 1220
MspI CCGG 1 cut(s) 701
MspR9I CCNGG 2 cut(s) 982, 1227
Mva1269I GAATGC 1 cut(s) 1097
MvaI CCWGG 2 cut(s) 982, 1227
MwoI GCNNNNNNNGC 2 cut(s) 848, 1323
NdeI CATATG 1 cut(s) 1129
NdeII GATC 6 cut(s) 128, 142, 270, 508, 542, 799
NlaIII CATG 8 cut(s) 466, 515, 660, 850, 1049, 1147, 1171, 1298
NlaIV GGNNCC 4 cut(s) 422, 1237, 1263, 1264
NmeAIII GCCGAG 1 cut(s) 473
NspI RCATGY 1 cut(s) 1171
PagI TCATGA 2 cut(s) 511, 1143
PciI ACATGT 1 cut(s) 1167
PciSI GCTCTTC 1 cut(s) 232
PctI GAATGC 1 cut(s) 1097
PfeI GAWTC 6 cut(s) 446, 622, 697, 814, 1033, 1250
PkrI GCNGC 2 cut(s) 789, 1319
PleI GAGTC 1 cut(s) 979
PpsI GAGTC 1 cut(s) 979
Ppu21I YACGTR 1 cut(s) 1332
PpuMI RGGWCCY 1 cut(s) 1262
PscI ACATGT 1 cut(s) 1167
Psp5II RGGWCCY 1 cut(s) 1262
Psp6I CCWGG 2 cut(s) 980, 1225
PspGI CCWGG 2 cut(s) 980, 1225
PspN4I GGNNCC 4 cut(s) 422, 1237, 1263, 1264
PspPI GGNCC 2 cut(s) 1236, 1262
PspPPI RGGWCCY 1 cut(s) 1262
PsuI RGATCY 2 cut(s) 128, 270
PvuII CAGCTG 2 cut(s) 790, 1320
RsaI GTAC 4 cut(s) 361, 728, 1017, 1330
RsaNI GTAC 4 cut(s) 360, 727, 1016, 1329
RseI CAYNNNNRTG 3 cut(s) 195, 393, 770
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 2 cut(s) 1083, 1221
SatI GCNGC 2 cut(s) 788, 1318
Sau3AI GATC 6 cut(s) 128, 142, 270, 508, 542, 799
Sau96I GGNCC 2 cut(s) 1236, 1262
ScaI AGTACT 1 cut(s) 728
SchI GAGTC 1 cut(s) 979
ScrFI CCNGG 2 cut(s) 982, 1227
SduI GDGCHC 2 cut(s) 404, 1151
SfaNI GCATC 3 cut(s) 622, 766, 871
SinI GGWCC 2 cut(s) 1236, 1262
SmiMI CAYNNNNRTG 3 cut(s) 195, 393, 770
SmlI CTYRAG 3 cut(s) 425, 554, 1220
SmoI CTYRAG 3 cut(s) 425, 554, 1220
SspMI CTAG 1 cut(s) 86
StyD4I CCNGG 2 cut(s) 980, 1225
StyI CCWWGG 2 cut(s) 531, 1151
TaiI ACGT 4 cut(s) 17, 335, 930, 1334
TaqI TCGA 3 cut(s) 141, 523, 677
TatI WGTACW 1 cut(s) 726
TfiI GAWTC 6 cut(s) 446, 622, 697, 814, 1033, 1250
Tru1I TTAA 2 cut(s) 1083, 1221
Tru9I TTAA 2 cut(s) 1083, 1221
TscAI CASTG 1 cut(s) 1246
TseI GCWGC 2 cut(s) 787, 1317
TspDTI ATGAA 6 cut(s) 294, 377, 722, 725, 806, 866
TspRI CASTG 1 cut(s) 1246
Vha464I CTTAAG 1 cut(s) 1220
VpaK11BI GGWCC 2 cut(s) 1236, 1262
XapI RAATTY 7 cut(s) 277, 289, 301, 352, 431, 519, 758
XceI RCATGY 1 cut(s) 1171
XspI CTAG 1 cut(s) 86
ZrmI AGTACT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.