Rh5BG106500

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011446
Physical Location & Seq
Reverse (-)
9768935 .. 9771774
2840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG106500.1

Sequence Viewer

Length: 1383 bp
ATGTGTCGTGGGAGAGCAACGTCCACTCCTCTGCTCTTTCAATCAAAGCTGGTCTGTTTCTCTCTGCTCTACCTCTTCACCTCTCTGTTTCTAGCTCTCTACACCTCTCTCTCCCAGACCAAATGCCTCTTCAGATCTTCACCTTTCGACCCAATTCAGTCCCCTCTCTTCTCCTACCCTCCCAATTATGGAGAGCACAAGTATGCAATCCCAACCCATCGTAAATCTTGCTCTTCCCCTGTCTATTTCTCAGATTATTGGAAGGTCTTGAAGGAGATCCAAAATTTGTGCAAGAATTCCTCTTGGAATTCATCGGTTTTGAGATATATGCAGGGAACGTCTGAGACTTTTGGAGGGAATTTCAGTACCCAAAATAGGATTTCCTATTTTGTTCATTACAATGAGAGCACTGAAGTCCCTTGTGGGTTCCTGAAGAAATTTCCAGTTAGTGATTCTGATACAATTGTCATGGAAGCGTGTAGAGGACTTGTAGTAGTCTCGTCTATCTTCAATGATCATGATAAAATTCGGCAACCTAAGGGGATTGGATCGAAAACCCTTGAGAATGTATGTTTCTTTATGTTTATAGATGAGATAACCCTCAAAGGATTTTACCAGCATCATTTGATTCCAGAGAAATCTGAAAACTATAAGATTGGTGCATGGAGGGTTGTCAAGGTTTTGAGCAAGGGATTGTATGAGAATCCGGCAATGAATGGTGCGATACCAAAGTATTTGGTTCATAGACTTTTCCCAAACGCCAAATTCAGCATTTGGGTGGATGCGAAAATGCAGCTGATGGTTGATCCATTACTTTTGATTCATGCACTTGTTGTGAATGAGAACGCCGACATGGCGATTTCAAAGCATCCTTACTATGTTCATACTATGGAAGAAGCAATGGCAACGGCGAGGTGGAAGAAATGGTGGGACGTTGATGGATTGAAGAGGCAAATGGAGACTTATTGTGAGAATGGTTTGCTCCCCTGGACTCCTGATAAGCTTCCTCATCCCTCAGATGTACCAGATAGTGCTTTGATTCTAAGGAAACATGGACTGAGGAGCAATCTATTCTCCTGCCTATTGTTTAATGAGATGGAGGCATTCAACCCAAGAGGCGAATTAGCGTTTGCATATGTGAGAGACCTCATGAGCCCAAGGCTGAAGCTGAACATGTTTTCAGTTGAAGTGTTTGAGCAAATTACTGTGGAATATAGGCACAACCTTAAGCCTGGAGTTGGAACCAGTGAAGGTGAATCTAAAGGAGGACCCAAAACCAAAAAGACCAAAAGCGCTGGCAATGATTTGTTGTATATCAATGGCAGCTGTTGCAGTAAGTGTCAGAGGTATCTTTTGGAGATGTGGGGGGAATCCCCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

460

Amino Acids

52.59

Weight (kDa)

8.85

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 113 - 407 1.6e-107 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46220
fragaria_vesca FvH4_3g05570 FvH4_3g05570
malus_domestica MD05G1284000.v1.1 MD10G1262300.v1.1
prunus_persica Prupe.4G080400_v2.0.a1 Prupe.4G080400_v2.0.a1
pyrus_communis pycom05g26310 pycom10g21850
rosa_chinensis RchiOBHm_Chr5g0014101
rosa_laevigata RLG00000032081
rosa_multiflora Rmu_sc0007767.1_g000016
rosa_roxburghii Rroxscaffold_1G00062530
rosa_rugosa Rorug05G0015700
rosa_samantha Rh5AG109600 Rh5BG106400 Rh5BG106500 Rh5CG118300 Rh5DG105100
rosa_wichuraiana Rw5G009570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 271, 556, 800
AcsI RAATTY 7 cut(s) 283, 295, 307, 358, 437, 525, 764
AcuI CTGAAG 4 cut(s) 115, 432, 452, 1184
AfaI GTAC 2 cut(s) 367, 1023
AfeI AGCGCT 1 cut(s) 1294
AfiI CCNNNNNNNGG 3 cut(s) 188, 375, 1238
AflII CTTAAG 1 cut(s) 1226
AflIII ACRYGT 1 cut(s) 1173
AgsI TTSAA 7 cut(s) 41, 271, 511, 864, 946, 1108, 1187
AjnI CCWGG 3 cut(s) 986, 1231, 1375
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AluBI AGCT 6 cut(s) 49, 95, 796, 1003, 1168, 1326
AluI AGCT 6 cut(s) 49, 95, 796, 1003, 1168, 1326
Alw21I GWGCWC 2 cut(s) 198, 410
Alw26I GTCTC 4 cut(s) 338, 502, 953, 1137
AlwI GGATC 3 cut(s) 271, 556, 800
Aor51HI AGCGCT 1 cut(s) 1294
ApeKI GCWGC 2 cut(s) 793, 1323
ApoI RAATTY 7 cut(s) 283, 295, 307, 358, 437, 525, 764
AspLEI GCGC 1 cut(s) 1295
AspS9I GGNCC 1 cut(s) 1268
AsuHPI GGTGA 3 cut(s) 70, 132, 1265
AvaII GGWCC 1 cut(s) 1268
AxyI CCTNAGG 1 cut(s) 537
BaeI ACNNNNGTAYC 4 cut(s) 450, 483, 716, 749
BanII GRGCYC 1 cut(s) 1157
Bbv12I GWGCWC 2 cut(s) 198, 410
BbvI GCAGC 2 cut(s) 805, 1335
BccI CCATC 4 cut(s) 225, 793, 932, 1090
BceAI ACGGC 1 cut(s) 924
BciT130I CCWGG 3 cut(s) 988, 1233, 1377
BclI TGATCA 1 cut(s) 514
BcoDI GTCTC 4 cut(s) 338, 502, 953, 1137
BfaI CTAG 1 cut(s) 92
BfoI RGCGCY 1 cut(s) 1296
BfrI CTTAAG 1 cut(s) 1226
BglI GCCNNNNNGGC 1 cut(s) 854
BglII AGATCT 1 cut(s) 134
BisI GCNGC 2 cut(s) 794, 1324
BlsI GCNGC 2 cut(s) 795, 1325
Bme1390I CCNGG 3 cut(s) 988, 1233, 1377
Bme18I GGWCC 1 cut(s) 1268
BmgT120I GGNCC 1 cut(s) 1268
BmiI GGNNCC 3 cut(s) 428, 1243, 1270
BmrFI CCNGG 3 cut(s) 988, 1233, 1377
BmsI GCATC 3 cut(s) 628, 772, 877
BpmI CTGGAG 1 cut(s) 1254
BpuEI CTTGAG 1 cut(s) 581
BsaI GGTCTC 1 cut(s) 1137
BsaJI CCNNGG 3 cut(s) 986, 1157, 1375
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 375, 1238
Bse1I ACTGG 2 cut(s) 443, 1245
Bse21I CCTNAGG 1 cut(s) 537
Bse3DI GCAATG 3 cut(s) 717, 906, 1306
BseBI CCWGG 3 cut(s) 988, 1233, 1377
BseDI CCNNGG 3 cut(s) 986, 1157, 1375
BseGI GGATG 3 cut(s) 787, 868, 1009
BseLI CCNNNNNNNGG 3 cut(s) 188, 375, 1238
BseMI GCAATG 3 cut(s) 717, 906, 1306
BseMII CTCAG 4 cut(s) 264, 333, 1029, 1049
BseNI ACTGG 2 cut(s) 443, 1245
BseRI GAGGAG 2 cut(s) 18, 1075
BseXI GCAGC 2 cut(s) 805, 1335
BsiHKAI GWGCWC 2 cut(s) 198, 410
BsiSI CCGG 1 cut(s) 707
BslFI GGGAC 3 cut(s) 145, 401, 944
BslI CCNNNNNNNGG 3 cut(s) 188, 375, 1238
BsmAI GTCTC 4 cut(s) 338, 502, 953, 1137
BsmFI GGGAC 3 cut(s) 145, 401, 944
BsmI GAATGC 1 cut(s) 1103
Bso31I GGTCTC 1 cut(s) 1137
Bsp1286I GDGCHC 3 cut(s) 198, 410, 1157
Bsp143I GATC 5 cut(s) 134, 276, 514, 548, 805
BspCNI CTCAG 4 cut(s) 263, 334, 1028, 1050
BspHI TCATGA 2 cut(s) 517, 1149
BspLI GGNNCC 3 cut(s) 428, 1243, 1270
BspPI GGATC 3 cut(s) 271, 556, 800
BspQI GCTCTTC 1 cut(s) 238
BspTI CTTAAG 1 cut(s) 1226
BspTNI GGTCTC 1 cut(s) 1137
BsrDI GCAATG 3 cut(s) 717, 906, 1306
BsrI ACTGG 2 cut(s) 443, 1245
BssECI CCNNGG 3 cut(s) 986, 1157, 1375
BssMI GATC 5 cut(s) 134, 276, 514, 548, 805
BssT1I CCWWGG 1 cut(s) 1157
Bst2UI CCWGG 3 cut(s) 988, 1233, 1377
Bst4CI ACNGT 1 cut(s) 1207
Bst6I CTCTTC 5 cut(s) 80, 134, 173, 238, 941
BstAFI CTTAAG 1 cut(s) 1226
BstAPI GCANNNNNTGC 1 cut(s) 1329
BstC8I GCNNGC 1 cut(s) 1297
BstDEI CTNAG 6 cut(s) 250, 342, 537, 1015, 1043, 1058
BstF5I GGATG 3 cut(s) 787, 868, 1009
BstH2I RGCGCY 1 cut(s) 1296
BstHHI GCGC 1 cut(s) 1295
BstKTI GATC 5 cut(s) 137, 279, 517, 551, 808
BstMAI GTCTC 4 cut(s) 338, 502, 953, 1137
BstMBI GATC 5 cut(s) 134, 276, 514, 548, 805
BstMWI GCNNNNNNNGC 2 cut(s) 854, 1329
BstNI CCWGG 3 cut(s) 988, 1233, 1377
BstNSI RCATGY 1 cut(s) 1177
BstSCI CCNGG 3 cut(s) 986, 1231, 1375
BstV1I GCAGC 2 cut(s) 805, 1335
BstX2I RGATCY 2 cut(s) 134, 276
BstYI RGATCY 2 cut(s) 134, 276
Bsu36I CCTNAGG 1 cut(s) 537
BtsCI GGATG 3 cut(s) 787, 868, 1009
BtsIMutI CAGTG 2 cut(s) 408, 1252
Cac8I GCNNGC 1 cut(s) 1297
CciI TCATGA 2 cut(s) 517, 1149
CfoI GCGC 1 cut(s) 1295
Cfr13I GGNCC 1 cut(s) 1268
Csp6I GTAC 2 cut(s) 366, 1022
CviAII CATG 8 cut(s) 469, 518, 663, 824, 853, 1052, 1150, 1174
CviJI RGCY 9 cut(s) 49, 95, 796, 1003, 1155, 1162, 1168, 1231, 1326
CviKI_1 RGCY 9 cut(s) 49, 95, 796, 1003, 1155, 1162, 1168, 1231, 1326
CviQI GTAC 2 cut(s) 366, 1022
DdeI CTNAG 6 cut(s) 250, 342, 537, 1015, 1043, 1058
DpnI GATC 5 cut(s) 136, 278, 516, 550, 807
DpnII GATC 5 cut(s) 134, 276, 514, 548, 805
Eam1104I CTCTTC 5 cut(s) 80, 134, 173, 238, 941
EarI CTCTTC 5 cut(s) 80, 134, 173, 238, 941
Eco130I CCWWGG 1 cut(s) 1157
Eco24I GRGCYC 1 cut(s) 1157
Eco31I GGTCTC 1 cut(s) 1137
Eco47I GGWCC 1 cut(s) 1268
Eco47III AGCGCT 1 cut(s) 1294
Eco57I CTGAAG 4 cut(s) 115, 432, 452, 1184
Eco81I CCTNAGG 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 1268
EcoRI GAATTC 2 cut(s) 295, 307
EcoRII CCWGG 3 cut(s) 986, 1231, 1375
EcoT14I CCWWGG 1 cut(s) 1157
EcoT38I GRGCYC 1 cut(s) 1157
ErhI CCWWGG 1 cut(s) 1157
FaeI CATG 8 cut(s) 472, 521, 666, 827, 856, 1055, 1153, 1177
FaqI GGGAC 3 cut(s) 145, 401, 944
FatI CATG 8 cut(s) 468, 517, 662, 823, 852, 1051, 1149, 1173
FauNDI CATATG 1 cut(s) 1135
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 2 cut(s) 794, 1324
FokI GGATG 3 cut(s) 794, 855, 996
FriOI GRGCYC 1 cut(s) 1157
Fsp4HI GCNGC 2 cut(s) 794, 1324
FspBI CTAG 1 cut(s) 92
GlaI GCGC 1 cut(s) 1294
GluI GCNGC 2 cut(s) 794, 1324
GsuI CTGGAG 1 cut(s) 1254
HaeII RGCGCY 1 cut(s) 1296
HapII CCGG 1 cut(s) 707
HhaI GCGC 1 cut(s) 1295
Hin1II CATG 8 cut(s) 472, 521, 666, 827, 856, 1055, 1153, 1177
Hin6I GCGC 1 cut(s) 1293
HinP1I GCGC 1 cut(s) 1293
HindIII AAGCTT 1 cut(s) 1001
HinfI GANTC 8 cut(s) 452, 628, 703, 820, 991, 1039, 1256, 1370
HpaII CCGG 1 cut(s) 707
HphI GGTGA 3 cut(s) 70, 132, 1265
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 7 cut(s) 134, 253, 343, 457, 643, 1018, 1344
Hpy188III TCNNGA 6 cut(s) 268, 430, 518, 632, 995, 1150
Hpy8I GTNNAC 1 cut(s) 24
HpyAV CCTTC 3 cut(s) 256, 265, 1244
HpyCH4III ACNGT 1 cut(s) 1207
HpyCH4IV ACGT 3 cut(s) 20, 338, 933
HpyCH4V TGCA 8 cut(s) 206, 291, 331, 662, 793, 827, 1133, 1332
HpyF10VI GCNNNNNNNGC 2 cut(s) 854, 1329
HpyF3I CTNAG 6 cut(s) 250, 342, 537, 1015, 1043, 1058
HpySE526I ACGT 3 cut(s) 20, 338, 933
Hsp92II CATG 8 cut(s) 472, 521, 666, 827, 856, 1055, 1153, 1177
HspAI GCGC 1 cut(s) 1293
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 5 cut(s) 134, 276, 514, 548, 805
LguI GCTCTTC 1 cut(s) 238
LmnI GCTCC 2 cut(s) 987, 1062
Lsp1109I GCAGC 2 cut(s) 805, 1335
LweI GCATC 3 cut(s) 628, 772, 877
MaeI CTAG 1 cut(s) 92
MaeII ACGT 3 cut(s) 20, 338, 933
MalI GATC 5 cut(s) 136, 278, 516, 550, 807
MboI GATC 5 cut(s) 134, 276, 514, 548, 805
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 2 cut(s) 134, 276
MhlI GDGCHC 3 cut(s) 198, 410, 1157
MlyI GAGTC 1 cut(s) 985
MmeI TCCRAC 1 cut(s) 1219
MseI TTAA 3 cut(s) 1089, 1227, 1381
MslI CAYNNNNRTG 3 cut(s) 201, 399, 776
MspA1I CMGCKG 2 cut(s) 796, 1326
MspCI CTTAAG 1 cut(s) 1226
MspI CCGG 1 cut(s) 707
MspR9I CCNGG 3 cut(s) 988, 1233, 1377
MunI CAATTG 1 cut(s) 462
Mva1269I GAATGC 1 cut(s) 1103
MvaI CCWGG 3 cut(s) 988, 1233, 1377
MwoI GCNNNNNNNGC 2 cut(s) 854, 1329
NdeI CATATG 1 cut(s) 1135
NdeII GATC 5 cut(s) 134, 276, 514, 548, 805
NlaIII CATG 8 cut(s) 472, 521, 666, 827, 856, 1055, 1153, 1177
NlaIV GGNNCC 3 cut(s) 428, 1243, 1270
NspI RCATGY 1 cut(s) 1177
PagI TCATGA 2 cut(s) 517, 1149
PciI ACATGT 1 cut(s) 1173
PciSI GCTCTTC 1 cut(s) 238
PctI GAATGC 1 cut(s) 1103
PfeI GAWTC 7 cut(s) 452, 628, 703, 820, 1039, 1256, 1370
PkrI GCNGC 2 cut(s) 795, 1325
PleI GAGTC 1 cut(s) 985
PpsI GAGTC 1 cut(s) 985
PpuMI RGGWCCY 1 cut(s) 1268
PscI ACATGT 1 cut(s) 1173
Psp5II RGGWCCY 1 cut(s) 1268
Psp6I CCWGG 3 cut(s) 986, 1231, 1375
PspGI CCWGG 3 cut(s) 986, 1231, 1375
PspN4I GGNNCC 3 cut(s) 428, 1243, 1270
PspPI GGNCC 1 cut(s) 1268
PspPPI RGGWCCY 1 cut(s) 1268
PsuI RGATCY 2 cut(s) 134, 276
PvuII CAGCTG 2 cut(s) 796, 1326
RsaI GTAC 2 cut(s) 367, 1023
RsaNI GTAC 2 cut(s) 366, 1022
RseI CAYNNNNRTG 3 cut(s) 201, 399, 776
SapI GCTCTTC 1 cut(s) 238
SaqAI TTAA 3 cut(s) 1089, 1227, 1381
SatI GCNGC 2 cut(s) 794, 1324
Sau3AI GATC 5 cut(s) 134, 276, 514, 548, 805
Sau96I GGNCC 1 cut(s) 1268
SchI GAGTC 1 cut(s) 985
ScrFI CCNGG 3 cut(s) 988, 1233, 1377
SduI GDGCHC 3 cut(s) 198, 410, 1157
SfaNI GCATC 3 cut(s) 628, 772, 877
SinI GGWCC 1 cut(s) 1268
SmiMI CAYNNNNRTG 3 cut(s) 201, 399, 776
SmlI CTYRAG 2 cut(s) 560, 1226
SmoI CTYRAG 2 cut(s) 560, 1226
SspMI CTAG 1 cut(s) 92
StyD4I CCNGG 3 cut(s) 986, 1231, 1375
StyI CCWWGG 1 cut(s) 1157
TaaI ACNGT 1 cut(s) 1207
TaiI ACGT 3 cut(s) 23, 341, 936
TaqI TCGA 2 cut(s) 147, 551
TfiI GAWTC 7 cut(s) 452, 628, 703, 820, 1039, 1256, 1370
Tru1I TTAA 3 cut(s) 1089, 1227, 1381
Tru9I TTAA 3 cut(s) 1089, 1227, 1381
TscAI CASTG 2 cut(s) 415, 1252
TseI GCWGC 2 cut(s) 793, 1323
TspDTI ATGAA 6 cut(s) 300, 383, 728, 731, 812, 872
TspRI CASTG 2 cut(s) 415, 1252
Vha464I CTTAAG 1 cut(s) 1226
VpaK11BI GGWCC 1 cut(s) 1268
XapI RAATTY 7 cut(s) 283, 295, 307, 358, 437, 525, 764
XceI RCATGY 1 cut(s) 1177
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.