Rh5CG118300

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011446
Physical Location & Seq
Reverse (-)
9753340 .. 9756497
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG118300.1

Sequence Viewer

Length: 1194 bp
ATGTGTGACATTGTGAACAGAATAAAGAAGAGTATAAAATCAAAGGCGAGCATCCTTAAGAAATGTGTCATGGGGAAAGTAACGTCCACTCCTCTGCTCTTTCAATCAAAGCTGGTCTGTTTCTCTCTGCTCTACCTCTTCACCTCTCTGTTTCTAGCTCTCTACACCTCTCTCTCCCAAACCAAATGCCTCTTCAGATCTTCACCTTTCGACCCAATTCAGCCCCCTCTCTTCTCCTACCCTCCCAATTATGGAGAACACAAATATGCAATCCCAACCCAACGTAAATCTTGCTCTTCCCCTGTCCATTTCTCAGGTACCATCAATTTTTTTTTTCTAGCTCTGTTTCTCTTCATTTTTGGAATTTGGTCATTTTATCTGTGGATTTTGGTAAATGGGTTTTGGTTTTTGGGGTCAGATTATTGGAAGGTCTTGAAGGAGATCCAAAATTTGTGCAAGAGTTCTTCGTGGAATTCATCGGTTTTGAGTTATATGCAGGGAAAGTCTGAGACTTTTGGAGGGAATTTCAGTACCCAAAATAGGATTTCCTATTTTGTTCATTACAATGAGAGCACTGAAATCCCTTGTGGGTTCCTGAAGAAATTTCCAGTTAGTGATTCTGATAGAATTGTCATGGAAGAGTGTAGAGGACTTGTAGTAGTCTCGGCTATCTTCAATGATCATGATAAAATTCGACAACCCAAGGGGATTGGATCAAAAACCCTTGAGAATGTATGTTTCTTTATGTTTATAGATGAGATAACCCTCAAAGGATTTTACCAGCATCATTTGATTCCAGAAAGATCTGAAAACTATAAGATTGGTGCATGGAGGGTTGTCAAGGTTTTGAGCAAGGGATTGTATGAGAATCCGGCAATGAATGGTGTGATACCAAAGTACTTGGTTCATAGACTTTTCCCAAACGCCAAATTCAGCATTTGGGTGGATGCGAAAATGCAGCTGATGGTTGATCCATTACTTTTGATTCACACACTTGTTGTGAATGAGAACGCCGACATGGCGATTTCAAAGCATCCTTACTATGTTCATACTATGGAAGAAGCAATGGCGACGGCGAGGTGGAAGAAATGGTGGGACGTTGATGGATTAAAGAGGCAAATGGAGACATATTGTGAAAATGGTTTGCTCCCCTGGACTCCTGATAAGCTTCCTTATCCCTCAGGTATTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

397

Amino Acids

45.87

Weight (kDa)

9.15

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 167 - 395 1.3e-79 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46220
fragaria_vesca FvH4_3g05570 FvH4_3g05570
malus_domestica MD05G1284000.v1.1 MD10G1262300.v1.1
prunus_persica Prupe.4G080400_v2.0.a1 Prupe.4G080400_v2.0.a1
pyrus_communis pycom05g26310 pycom10g21850
rosa_chinensis RchiOBHm_Chr5g0014101
rosa_laevigata RLG00000032081
rosa_multiflora Rmu_sc0007767.1_g000016
rosa_roxburghii Rroxscaffold_1G00062530
rosa_rugosa Rorug05G0015700
rosa_samantha Rh5AG109600 Rh5BG106400 Rh5BG106500 Rh5CG118300 Rh5DG105100
rosa_wichuraiana Rw5G009570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 317
AccB1I GGYRCC 1 cut(s) 317
AclWI GGATC 3 cut(s) 436, 721, 965
AcsI RAATTY 7 cut(s) 363, 448, 472, 523, 602, 690, 929
AcuI CTGAAG 2 cut(s) 178, 617
AfaI GTAC 3 cut(s) 319, 532, 899
AfiI CCNNNNNNNGG 2 cut(s) 251, 540
AflII CTTAAG 1 cut(s) 56
AgsI TTSAA 4 cut(s) 104, 436, 676, 1029
AjnI CCWGG 1 cut(s) 1151
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AluBI AGCT 5 cut(s) 112, 158, 341, 961, 1168
AluI AGCT 5 cut(s) 112, 158, 341, 961, 1168
Alw21I GWGCWC 1 cut(s) 575
Alw26I GTCTC 3 cut(s) 503, 667, 1118
AlwI GGATC 3 cut(s) 436, 721, 965
ApeKI GCWGC 1 cut(s) 958
ApoI RAATTY 7 cut(s) 363, 448, 472, 523, 602, 690, 929
Asp718I GGTACC 1 cut(s) 317
AsuHPI GGTGA 2 cut(s) 133, 195
AxyI CCTNAGG 1 cut(s) 1180
BaeI ACNNNNGTAYC 2 cut(s) 881, 914
BanI GGYRCC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 1 cut(s) 970
BccI CCATC 3 cut(s) 329, 958, 1097
BceAI ACGGC 1 cut(s) 1089
BciT130I CCWGG 1 cut(s) 1153
BclI TGATCA 1 cut(s) 679
BcoDI GTCTC 3 cut(s) 503, 667, 1118
BfaI CTAG 2 cut(s) 155, 338
BfrI CTTAAG 1 cut(s) 56
BglI GCCNNNNNGGC 1 cut(s) 1019
BglII AGATCT 2 cut(s) 197, 803
BisI GCNGC 1 cut(s) 959
BlsI GCNGC 1 cut(s) 960
BmcAI AGTACT 1 cut(s) 899
Bme1390I CCNGG 1 cut(s) 1153
BmiI GGNNCC 2 cut(s) 319, 593
BmrFI CCNGG 1 cut(s) 1153
BmsI GCATC 4 cut(s) 60, 793, 937, 1042
BpuEI CTTGAG 1 cut(s) 746
BsaJI CCNNGG 2 cut(s) 702, 1151
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 540
Bse1I ACTGG 1 cut(s) 608
Bse21I CCTNAGG 1 cut(s) 1180
Bse3DI GCAATG 2 cut(s) 882, 1071
BseBI CCWGG 1 cut(s) 1153
BseDI CCNNGG 2 cut(s) 702, 1151
BseGI GGATG 3 cut(s) 51, 952, 1033
BseLI CCNNNNNNNGG 2 cut(s) 251, 540
BseMI GCAATG 2 cut(s) 882, 1071
BseMII CTCAG 3 cut(s) 327, 498, 1194
BseNI ACTGG 1 cut(s) 608
BseRI GAGGAG 1 cut(s) 81
BseXI GCAGC 1 cut(s) 970
BshNI GGYRCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 575
BsiSI CCGG 1 cut(s) 872
BslFI GGGAC 1 cut(s) 1109
BslI CCNNNNNNNGG 2 cut(s) 251, 540
BsmAI GTCTC 3 cut(s) 503, 667, 1118
BsmFI GGGAC 1 cut(s) 1109
Bsp1286I GDGCHC 1 cut(s) 575
Bsp143I GATC 6 cut(s) 197, 441, 679, 713, 803, 970
BspCNI CTCAG 3 cut(s) 326, 499, 1193
BspHI TCATGA 1 cut(s) 682
BspLI GGNNCC 2 cut(s) 319, 593
BspPI GGATC 3 cut(s) 436, 721, 965
BspQI GCTCTTC 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 317
BspTI CTTAAG 1 cut(s) 56
BsrDI GCAATG 2 cut(s) 882, 1071
BsrI ACTGG 1 cut(s) 608
BssECI CCNNGG 2 cut(s) 702, 1151
BssMI GATC 6 cut(s) 197, 441, 679, 713, 803, 970
BssT1I CCWWGG 1 cut(s) 702
Bst2UI CCWGG 1 cut(s) 1153
Bst6I CTCTTC 7 cut(s) 23, 143, 197, 236, 301, 356, 633
BstAFI CTTAAG 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 3 cut(s) 313, 507, 1180
BstF5I GGATG 3 cut(s) 51, 952, 1033
BstKTI GATC 6 cut(s) 200, 444, 682, 716, 806, 973
BstMAI GTCTC 3 cut(s) 503, 667, 1118
BstMBI GATC 6 cut(s) 197, 441, 679, 713, 803, 970
BstMWI GCNNNNNNNGC 1 cut(s) 1019
BstNI CCWGG 1 cut(s) 1153
BstSCI CCNGG 1 cut(s) 1151
BstV1I GCAGC 1 cut(s) 970
BstX2I RGATCY 3 cut(s) 197, 441, 803
BstYI RGATCY 3 cut(s) 197, 441, 803
Bsu36I CCTNAGG 1 cut(s) 1180
BtsCI GGATG 3 cut(s) 51, 952, 1033
BtsIMutI CAGTG 1 cut(s) 573
Cac8I GCNNGC 1 cut(s) 49
CciI TCATGA 1 cut(s) 682
Csp6I GTAC 3 cut(s) 318, 531, 898
CviAII CATG 5 cut(s) 70, 634, 683, 828, 1018
CviJI RGCY 7 cut(s) 112, 158, 223, 341, 668, 961, 1168
CviKI_1 RGCY 7 cut(s) 112, 158, 223, 341, 668, 961, 1168
CviQI GTAC 3 cut(s) 318, 531, 898
DdeI CTNAG 3 cut(s) 313, 507, 1180
DpnI GATC 6 cut(s) 199, 443, 681, 715, 805, 972
DpnII GATC 6 cut(s) 197, 441, 679, 713, 803, 970
Eam1104I CTCTTC 7 cut(s) 23, 143, 197, 236, 301, 356, 633
EarI CTCTTC 7 cut(s) 23, 143, 197, 236, 301, 356, 633
Eco130I CCWWGG 1 cut(s) 702
Eco57I CTGAAG 2 cut(s) 178, 617
Eco81I CCTNAGG 1 cut(s) 1180
EcoRI GAATTC 1 cut(s) 472
EcoRII CCWGG 1 cut(s) 1151
EcoT14I CCWWGG 1 cut(s) 702
ErhI CCWWGG 1 cut(s) 702
FaeI CATG 5 cut(s) 73, 637, 686, 831, 1021
FaqI GGGAC 1 cut(s) 1109
FatI CATG 5 cut(s) 69, 633, 682, 827, 1017
FbaI TGATCA 1 cut(s) 679
Fnu4HI GCNGC 1 cut(s) 959
FokI GGATG 3 cut(s) 38, 959, 1020
Fsp4HI GCNGC 1 cut(s) 959
FspBI CTAG 2 cut(s) 155, 338
GluI GCNGC 1 cut(s) 959
HapII CCGG 1 cut(s) 872
Hin1II CATG 5 cut(s) 73, 637, 686, 831, 1021
HindIII AAGCTT 1 cut(s) 1166
HinfI GANTC 5 cut(s) 617, 793, 868, 985, 1156
HpaII CCGG 1 cut(s) 872
HphI GGTGA 2 cut(s) 133, 195
Hpy166II GTNNAC 2 cut(s) 16, 87
Hpy188I TCNGA 5 cut(s) 197, 418, 508, 622, 808
Hpy188III TCNNGA 5 cut(s) 433, 595, 683, 797, 1160
Hpy8I GTNNAC 2 cut(s) 16, 87
Hpy99I CGWCG 1 cut(s) 1075
HpyAV CCTTC 2 cut(s) 421, 430
HpyCH4IV ACGT 3 cut(s) 83, 283, 1098
HpyCH4V TGCA 5 cut(s) 269, 456, 496, 827, 958
HpyF10VI GCNNNNNNNGC 1 cut(s) 1019
HpyF3I CTNAG 3 cut(s) 313, 507, 1180
HpySE526I ACGT 3 cut(s) 83, 283, 1098
Hsp92II CATG 5 cut(s) 73, 637, 686, 831, 1021
KpnI GGTACC 1 cut(s) 321
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 6 cut(s) 197, 441, 679, 713, 803, 970
LguI GCTCTTC 1 cut(s) 301
LmnI GCTCC 1 cut(s) 1152
Lsp1109I GCAGC 1 cut(s) 970
LweI GCATC 4 cut(s) 60, 793, 937, 1042
MaeI CTAG 2 cut(s) 155, 338
MaeII ACGT 3 cut(s) 83, 283, 1098
MaeIII GTNAC 2 cut(s) 5, 79
MalI GATC 6 cut(s) 199, 443, 681, 715, 805, 972
MboI GATC 6 cut(s) 197, 441, 679, 713, 803, 970
MflI RGATCY 3 cut(s) 197, 441, 803
MhlI GDGCHC 1 cut(s) 575
MlyI GAGTC 1 cut(s) 1150
MseI TTAA 2 cut(s) 57, 1109
MslI CAYNNNNRTG 3 cut(s) 264, 564, 941
MspA1I CMGCKG 1 cut(s) 961
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 1 cut(s) 872
MspR9I CCNGG 1 cut(s) 1153
MvaI CCWGG 1 cut(s) 1153
MwoI GCNNNNNNNGC 1 cut(s) 1019
NdeII GATC 6 cut(s) 197, 441, 679, 713, 803, 970
NlaIII CATG 5 cut(s) 73, 637, 686, 831, 1021
NlaIV GGNNCC 2 cut(s) 319, 593
NmeAIII GCCGAG 1 cut(s) 644
NmuCI GTSAC 1 cut(s) 5
PagI TCATGA 1 cut(s) 682
PciSI GCTCTTC 1 cut(s) 301
PfeI GAWTC 4 cut(s) 617, 793, 868, 985
PkrI GCNGC 1 cut(s) 960
PleI GAGTC 1 cut(s) 1150
PpsI GAGTC 1 cut(s) 1150
Psp6I CCWGG 1 cut(s) 1151
PspGI CCWGG 1 cut(s) 1151
PspN4I GGNNCC 2 cut(s) 319, 593
PsuI RGATCY 3 cut(s) 197, 441, 803
PvuII CAGCTG 1 cut(s) 961
RsaI GTAC 3 cut(s) 319, 532, 899
RsaNI GTAC 3 cut(s) 318, 531, 898
RseI CAYNNNNRTG 3 cut(s) 264, 564, 941
SapI GCTCTTC 1 cut(s) 301
SaqAI TTAA 2 cut(s) 57, 1109
SatI GCNGC 1 cut(s) 959
Sau3AI GATC 6 cut(s) 197, 441, 679, 713, 803, 970
ScaI AGTACT 1 cut(s) 899
SchI GAGTC 1 cut(s) 1150
ScrFI CCNGG 1 cut(s) 1153
SduI GDGCHC 1 cut(s) 575
SfaNI GCATC 4 cut(s) 60, 793, 937, 1042
SmiMI CAYNNNNRTG 3 cut(s) 264, 564, 941
SmlI CTYRAG 2 cut(s) 56, 725
SmoI CTYRAG 2 cut(s) 56, 725
SspMI CTAG 2 cut(s) 155, 338
StyD4I CCNGG 1 cut(s) 1151
StyI CCWWGG 1 cut(s) 702
TaiI ACGT 3 cut(s) 86, 286, 1101
TaqI TCGA 2 cut(s) 210, 694
TatI WGTACW 1 cut(s) 897
TfiI GAWTC 4 cut(s) 617, 793, 868, 985
Tru1I TTAA 2 cut(s) 57, 1109
Tru9I TTAA 2 cut(s) 57, 1109
TscAI CASTG 1 cut(s) 580
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 1 cut(s) 958
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 6 cut(s) 343, 465, 548, 893, 896, 1037
TspRI CASTG 1 cut(s) 580
Vha464I CTTAAG 1 cut(s) 56
XapI RAATTY 7 cut(s) 363, 448, 472, 523, 602, 690, 929
XspI CTAG 2 cut(s) 155, 338
ZrmI AGTACT 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.