pycom05g26310

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011446
Physical Location & Seq
Forward (+)
27661659 .. 27664646
2988 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g26310.3

Sequence Viewer

Length: 1377 bp
ATGGGGAAGGCAAGTTCCACTCCTCTCCTCTTTCAATCAAAGTTGGTGTGTTTCTCTCTGCTCTACCTCTTCACCTCTCTGTTTCTAGCTCTCTACACCTCTCTCTCCCAAACCAAATGCCTCTTCAGATCTTCCCCCTTCGACCCGATTCAGGCCCCTCTCTTCTCCTACCCTTCCACCTACGGCGAGCACAAGTATGCAATCCCAACCCACCGCACCTCTTGCTCTTCTCCCGTCTATTTTTCAGAATATTGGATGGTCTTGAAGGAGATCCAGAATTTGTGTAAGAATTCTTCGTGGTCACCGTCGGTTTTGAGGTATTTGCAGGGAAAGGCTGAGACATTTGCAGGAAATTTAAGTACCCAAGCGAGGATTCCCTATTTTGTTCATTACAATGAGAGCACTGAAATCCCTTGTGGATTCTTGAAGAAATTTCCGATTAGTGATTCTGATAGAACGGCTATGGAAAACTGTAAAGGAGTTGTAGTGGTCTCGGCGATCTTCAATGACCATGATAAAATCCGACAACCCCGAGGCCTTGGATCGGAGACCCTAGACAATGTATGCTTTTTTCTGTTCATAGATGATGTAACCCTAAAAGGACTTGACTACCATAGATTGATTCCGAGAAAATCCCAAAATTTTAAGATTGGCGCGTGGAGGGTTGTGAAAGTTTTGAGCAAGAAATTGTATGAGAATCCTGCAATGAACGGTGTGATTCCAAAATATTTAGTCCATAGGCTTTTCCCAAACTCGAAATTCAGCATTTGGATCGATGCGAAGCTTCAGCTAGTGATCGATCCGTTGCTGTTGATTCATTCACTTGTGGTGTCTGACAACGCAGACATGGCGATTTCGAAGCATCCTTACTATGTACATACTATGGAGGAGGCAATGGCGACGGCGAGGTGGCAGAAATGGAGTGATGTTGATGGATTGAAGAGGCAGATGGAGACTTATTGTGAGAATGGATTGCTTCCTTGGACCCCCAGCAAGCTGCCTTATCCCTCAGACGTACCAGACAGTGCTCTAATCCTGAGGAAACATGGATTGAGGAGCAACCTATTCTCCTGCCTGTTGTTCAATGAGTTGGAAGCATTTAACCCTAGAGACCAATTGGCTTTTGCATACGTGAGAGACCTCATGAGCCCAAGGCTGAAGATCAACATGTTTGACGTCGAAGTGTTTGAGCAAATTGTTGTGGAATACAGGCACAACATCAAGCGTAAAGGTGGGGACAGCGGAAGCCAAATGGGGTCCAAAGCCCCCAAAACCCAAAAGACCAAAAGGGCAAGCCCTGATTTGTTGTATGTTAATGGCAGCTGCTGCAGCAAGTGCCAGAAGTATCTTTTGGAGATGTGGGGTGAGGAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

459

Amino Acids

52.39

Weight (kDa)

9.0

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46220
fragaria_vesca FvH4_3g05570 FvH4_3g05570
malus_domestica MD05G1284000.v1.1 MD10G1262300.v1.1
prunus_persica Prupe.4G080400_v2.0.a1 Prupe.4G080400_v2.0.a1
pyrus_communis pycom05g26310 pycom10g21850
rosa_chinensis RchiOBHm_Chr5g0014101
rosa_laevigata RLG00000032081
rosa_multiflora Rmu_sc0007767.1_g000016
rosa_roxburghii Rroxscaffold_1G00062530
rosa_rugosa Rorug05G0015700
rosa_samantha Rh5AG109600 Rh5BG106400 Rh5BG106500 Rh5CG118300 Rh5DG105100
rosa_wichuraiana Rw5G009570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1179
AccII CGCG 1 cut(s) 656
AciI CCGC 2 cut(s) 214, 1242
AclWI GGATC 4 cut(s) 265, 550, 779, 794
AcsI RAATTY 6 cut(s) 277, 289, 352, 431, 640, 758
AcuI CTGAAG 3 cut(s) 109, 770, 1178
AcyI GRCGYC 1 cut(s) 1176
AfaI GTAC 3 cut(s) 361, 876, 1017
AfiI CCNNNNNNNGG 3 cut(s) 151, 369, 544
AflIII ACRYGT 1 cut(s) 1167
AgsI TTSAA 6 cut(s) 35, 265, 427, 505, 940, 1084
AjuI GAANNNNNNNTTGG 6 cut(s) 107, 139, 357, 389, 1334, 1366
AloI GAACNNNNNNTCC 1 cut(s) 30
AluBI AGCT 5 cut(s) 89, 784, 790, 997, 1323
AluI AGCT 5 cut(s) 89, 784, 790, 997, 1323
Alw21I GWGCWC 3 cut(s) 192, 404, 1030
Alw26I GTCTC 6 cut(s) 332, 496, 542, 947, 1104, 1131
AlwI GGATC 4 cut(s) 265, 550, 779, 794
AlwNI CAGNNNCTG 1 cut(s) 1326
Ama87I CYCGRG 1 cut(s) 531
AoxI GGCC 2 cut(s) 153, 535
ApeKI GCWGC 5 cut(s) 997, 1320, 1323, 1326, 1329
ApoI RAATTY 6 cut(s) 277, 289, 352, 431, 640, 758
AspLEI GCGC 1 cut(s) 656
AspS9I GGNCC 3 cut(s) 154, 984, 1257
AsuHPI GGTGA 3 cut(s) 64, 294, 1376
AsuII TTCGAA 1 cut(s) 857
AvaI CYCGRG 1 cut(s) 531
AvaII GGWCC 2 cut(s) 984, 1257
AxyI CCTNAGG 1 cut(s) 1037
BanII GRGCYC 1 cut(s) 1151
BarI GAAGNNNNNNTAC 4 cut(s) 277, 309, 851, 883
Bbv12I GWGCWC 3 cut(s) 192, 404, 1030
BbvI GCAGC 5 cut(s) 984, 1310, 1313, 1332, 1341
BccI CCATC 3 cut(s) 250, 926, 943
BceAI ACGGC 3 cut(s) 199, 474, 918
BcgI CGANNNNNNTGC 2 cut(s) 754, 788
BcoDI GTCTC 6 cut(s) 332, 496, 542, 947, 1104, 1131
BfaI CTAG 4 cut(s) 86, 554, 791, 1107
BfmI CTRYAG 1 cut(s) 1327
BglII AGATCT 1 cut(s) 128
BisI GCNGC 5 cut(s) 998, 1321, 1324, 1327, 1330
BlsI GCNGC 5 cut(s) 999, 1322, 1325, 1328, 1331
Bme18I GGWCC 2 cut(s) 984, 1257
BmeT110I CYCGRG 1 cut(s) 531
BmgT120I GGNCC 3 cut(s) 154, 984, 1257
BmiI GGNNCC 3 cut(s) 156, 986, 1258
BmsI GCATC 2 cut(s) 766, 871
Bpu14I TTCGAA 1 cut(s) 857
Bsa29I ATCGAT 2 cut(s) 774, 798
BsaAI YACGTR 1 cut(s) 1132
BsaHI GRCGYC 1 cut(s) 1176
BsaI GGTCTC 4 cut(s) 496, 542, 1104, 1131
BsaJI CCNNGG 4 cut(s) 532, 538, 980, 1151
BsaXI ACNNNNNCTCC 4 cut(s) 471, 501, 1052, 1082
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 369, 544
Bse21I CCTNAGG 1 cut(s) 1037
Bse3DI GCAATG 2 cut(s) 711, 900
BseCI ATCGAT 2 cut(s) 774, 798
BseDI CCNNGG 4 cut(s) 532, 538, 980, 1151
BseGI GGATG 2 cut(s) 261, 862
BseLI CCNNNNNNNGG 3 cut(s) 151, 369, 544
BseMI GCAATG 2 cut(s) 711, 900
BseMII CTCAG 3 cut(s) 327, 1023, 1028
BseRI GAGGAG 4 cut(s) 12, 17, 902, 1069
BseXI GCAGC 5 cut(s) 984, 1310, 1313, 1332, 1341
BseYI CCCAGC 1 cut(s) 989
Bsh1236I CGCG 1 cut(s) 656
BshFI GGCC 2 cut(s) 155, 537
BshVI ATCGAT 2 cut(s) 774, 798
BsiHKAI GWGCWC 3 cut(s) 192, 404, 1030
BsiHKCI CYCGRG 1 cut(s) 531
BslFI GGGAC 1 cut(s) 1250
BslI CCNNNNNNNGG 3 cut(s) 151, 369, 544
BsmAI GTCTC 6 cut(s) 332, 496, 542, 947, 1104, 1131
BsmFI GGGAC 1 cut(s) 1250
BsnI GGCC 2 cut(s) 155, 537
Bso31I GGTCTC 4 cut(s) 496, 542, 1104, 1131
BsoBI CYCGRG 1 cut(s) 531
Bsp119I TTCGAA 1 cut(s) 857
Bsp1286I GDGCHC 4 cut(s) 192, 404, 1030, 1151
Bsp1407I TGTACA 1 cut(s) 874
Bsp143I GATC 8 cut(s) 128, 270, 498, 542, 771, 795, 799, 1161
BspACI CCGC 2 cut(s) 214, 1242
BspANI GGCC 2 cut(s) 155, 537
BspCNI CTCAG 3 cut(s) 328, 1022, 1029
BspDI ATCGAT 2 cut(s) 774, 798
BspFNI CGCG 1 cut(s) 656
BspHI TCATGA 1 cut(s) 1143
BspLI GGNNCC 3 cut(s) 156, 986, 1258
BspMAI CTGCAG 1 cut(s) 1331
BspPI GGATC 4 cut(s) 265, 550, 779, 794
BspQI GCTCTTC 1 cut(s) 232
BspT104I TTCGAA 1 cut(s) 857
BspTNI GGTCTC 4 cut(s) 496, 542, 1104, 1131
BsrDI GCAATG 2 cut(s) 711, 900
BsrGI TGTACA 1 cut(s) 874
BssECI CCNNGG 4 cut(s) 532, 538, 980, 1151
BssMI GATC 8 cut(s) 128, 270, 498, 542, 771, 795, 799, 1161
BssNI GRCGYC 1 cut(s) 1176
BssT1I CCWWGG 3 cut(s) 538, 980, 1151
Bst4CI ACNGT 4 cut(s) 306, 473, 713, 1025
Bst6I CTCTTC 5 cut(s) 74, 128, 167, 232, 935
BstACI GRCGYC 1 cut(s) 1176
BstAPI GCANNNNNTGC 3 cut(s) 222, 1326, 1335
BstAUI TGTACA 1 cut(s) 874
BstBAI YACGTR 1 cut(s) 1132
BstBI TTCGAA 1 cut(s) 857
BstC8I GCNNGC 3 cut(s) 188, 995, 1294
BstDEI CTNAG 3 cut(s) 336, 1009, 1037
BstEII GGTNACC 1 cut(s) 300
BstF5I GGATG 2 cut(s) 261, 862
BstFNI CGCG 1 cut(s) 656
BstHHI GCGC 1 cut(s) 656
BstKTI GATC 8 cut(s) 131, 273, 501, 545, 774, 798, 802, 1164
BstMAI GTCTC 6 cut(s) 332, 496, 542, 947, 1104, 1131
BstMBI GATC 8 cut(s) 128, 270, 498, 542, 771, 795, 799, 1161
BstMWI GCNNNNNNNGC 5 cut(s) 222, 848, 1326, 1329, 1335
BstNSI RCATGY 1 cut(s) 1171
BstPI GGTNACC 1 cut(s) 300
BstSFI CTRYAG 1 cut(s) 1327
BstUI CGCG 1 cut(s) 656
BstV1I GCAGC 5 cut(s) 984, 1310, 1313, 1332, 1341
BstX2I RGATCY 2 cut(s) 128, 270
BstYI RGATCY 2 cut(s) 128, 270
Bsu15I ATCGAT 2 cut(s) 774, 798
Bsu36I CCTNAGG 1 cut(s) 1037
BsuRI GGCC 2 cut(s) 155, 537
BsuTUI ATCGAT 2 cut(s) 774, 798
BtsCI GGATG 2 cut(s) 261, 862
BtsIMutI CAGTG 2 cut(s) 402, 1030
Cac8I GCNNGC 3 cut(s) 188, 995, 1294
CaiI CAGNNNCTG 1 cut(s) 1326
CciI TCATGA 1 cut(s) 1143
CfoI GCGC 1 cut(s) 656
Cfr13I GGNCC 3 cut(s) 154, 984, 1257
ClaI ATCGAT 2 cut(s) 774, 798
Csp6I GTAC 3 cut(s) 360, 875, 1016
CviAII CATG 5 cut(s) 512, 847, 1046, 1144, 1168
CviQI GTAC 3 cut(s) 360, 875, 1016
DdeI CTNAG 3 cut(s) 336, 1009, 1037
DpnI GATC 8 cut(s) 130, 272, 500, 544, 773, 797, 801, 1163
DpnII GATC 8 cut(s) 128, 270, 498, 542, 771, 795, 799, 1161
Eam1104I CTCTTC 5 cut(s) 74, 128, 167, 232, 935
EarI CTCTTC 5 cut(s) 74, 128, 167, 232, 935
Eco130I CCWWGG 3 cut(s) 538, 980, 1151
Eco147I AGGCCT 1 cut(s) 537
Eco24I GRGCYC 1 cut(s) 1151
Eco31I GGTCTC 4 cut(s) 496, 542, 1104, 1131
Eco47I GGWCC 2 cut(s) 984, 1257
Eco57I CTGAAG 3 cut(s) 109, 770, 1178
Eco81I CCTNAGG 1 cut(s) 1037
Eco88I CYCGRG 1 cut(s) 531
Eco91I GGTNACC 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 154
EcoO65I GGTNACC 1 cut(s) 300
EcoRI GAATTC 1 cut(s) 289
EcoT14I CCWWGG 3 cut(s) 538, 980, 1151
EcoT38I GRGCYC 1 cut(s) 1151
ErhI CCWWGG 3 cut(s) 538, 980, 1151
FaeI CATG 5 cut(s) 515, 850, 1049, 1147, 1171
FaqI GGGAC 1 cut(s) 1250
FatI CATG 5 cut(s) 511, 846, 1045, 1143, 1167
Fnu4HI GCNGC 5 cut(s) 998, 1321, 1324, 1327, 1330
FokI GGATG 2 cut(s) 268, 849
FriOI GRGCYC 1 cut(s) 1151
Fsp4HI GCNGC 5 cut(s) 998, 1321, 1324, 1327, 1330
FspBI CTAG 4 cut(s) 86, 554, 791, 1107
GlaI GCGC 1 cut(s) 655
GluI GCNGC 5 cut(s) 998, 1321, 1324, 1327, 1330
GsaI CCCAGC 1 cut(s) 993
HaeIII GGCC 2 cut(s) 155, 537
HhaI GCGC 1 cut(s) 656
Hin1I GRCGYC 1 cut(s) 1176
Hin1II CATG 5 cut(s) 515, 850, 1049, 1147, 1171
Hin6I GCGC 1 cut(s) 654
HinP1I GCGC 1 cut(s) 654
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 8 cut(s) 148, 373, 420, 446, 622, 697, 718, 814
HphI GGTGA 3 cut(s) 64, 294, 1376
Hpy188I TCNGA 9 cut(s) 128, 247, 438, 451, 524, 547, 627, 835, 1012
Hpy188III TCNNGA 5 cut(s) 262, 274, 424, 1036, 1144
Hpy99I CGWCG 3 cut(s) 310, 904, 1181
HpyAV CCTTC 3 cut(s) 148, 183, 259
HpyCH4III ACNGT 4 cut(s) 306, 473, 713, 1025
HpyCH4IV ACGT 3 cut(s) 1014, 1131, 1176
HpyCH4V TGCA 6 cut(s) 200, 325, 347, 704, 1127, 1329
HpyF10VI GCNNNNNNNGC 5 cut(s) 222, 848, 1326, 1329, 1335
HpyF3I CTNAG 3 cut(s) 336, 1009, 1037
HpySE526I ACGT 3 cut(s) 1014, 1131, 1176
Hsp92I GRCGYC 1 cut(s) 1176
Hsp92II CATG 5 cut(s) 515, 850, 1049, 1147, 1171
HspAI GCGC 1 cut(s) 654
Kzo9I GATC 8 cut(s) 128, 270, 498, 542, 771, 795, 799, 1161
LguI GCTCTTC 1 cut(s) 232
LmnI GCTCC 1 cut(s) 1056
Lsp1109I GCAGC 5 cut(s) 984, 1310, 1313, 1332, 1341
LweI GCATC 2 cut(s) 766, 871
MaeI CTAG 4 cut(s) 86, 554, 791, 1107
MaeII ACGT 3 cut(s) 1014, 1131, 1176
MaeIII GTNAC 2 cut(s) 300, 589
MalI GATC 8 cut(s) 130, 272, 500, 544, 773, 797, 801, 1163
MboI GATC 8 cut(s) 128, 270, 498, 542, 771, 795, 799, 1161
MfeI CAATTG 1 cut(s) 1115
MflI RGATCY 2 cut(s) 128, 270
MhlI GDGCHC 4 cut(s) 192, 404, 1030, 1151
MluCI AATT 9 cut(s) 277, 289, 352, 431, 640, 686, 758, 1115, 1194
MmeI TCCRAC 2 cut(s) 547, 1071
MseI TTAA 4 cut(s) 356, 645, 1101, 1314
MslI CAYNNNNRTG 2 cut(s) 195, 393
MspA1I CMGCKG 2 cut(s) 1242, 1323
MunI CAATTG 1 cut(s) 1115
MvnI CGCG 1 cut(s) 656
MwoI GCNNNNNNNGC 5 cut(s) 222, 848, 1326, 1329, 1335
NdeII GATC 8 cut(s) 128, 270, 498, 542, 771, 795, 799, 1161
NlaIII CATG 5 cut(s) 515, 850, 1049, 1147, 1171
NlaIV GGNNCC 3 cut(s) 156, 986, 1258
NmeAIII GCCGAG 1 cut(s) 473
NmuCI GTSAC 1 cut(s) 300
NspI RCATGY 1 cut(s) 1171
NspV TTCGAA 1 cut(s) 857
PagI TCATGA 1 cut(s) 1143
PceI AGGCCT 1 cut(s) 537
PciI ACATGT 1 cut(s) 1167
PciSI GCTCTTC 1 cut(s) 232
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PfeI GAWTC 8 cut(s) 148, 373, 420, 446, 622, 697, 718, 814
PkrI GCNGC 5 cut(s) 999, 1322, 1325, 1328, 1331
Ppu21I YACGTR 1 cut(s) 1132
PscI ACATGT 1 cut(s) 1167
PspEI GGTNACC 1 cut(s) 300
PspFI CCCAGC 1 cut(s) 989
PspN4I GGNNCC 3 cut(s) 156, 986, 1258
PspPI GGNCC 3 cut(s) 154, 984, 1257
PstI CTGCAG 1 cut(s) 1331
PstNI CAGNNNCTG 1 cut(s) 1326
PsuI RGATCY 2 cut(s) 128, 270
PvuII CAGCTG 1 cut(s) 1323
RsaI GTAC 3 cut(s) 361, 876, 1017
RsaNI GTAC 3 cut(s) 360, 875, 1016
RseI CAYNNNNRTG 2 cut(s) 195, 393
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 4 cut(s) 356, 645, 1101, 1314
SatI GCNGC 5 cut(s) 998, 1321, 1324, 1327, 1330
Sau3AI GATC 8 cut(s) 128, 270, 498, 542, 771, 795, 799, 1161
Sau96I GGNCC 3 cut(s) 154, 984, 1257
SduI GDGCHC 4 cut(s) 192, 404, 1030, 1151
SfaNI GCATC 2 cut(s) 766, 871
SfcI CTRYAG 1 cut(s) 1327
SfuI TTCGAA 1 cut(s) 857
SinI GGWCC 2 cut(s) 984, 1257
SmiMI CAYNNNNRTG 2 cut(s) 195, 393
Sse9I AATT 9 cut(s) 277, 289, 352, 431, 640, 686, 758, 1115, 1194
SseBI AGGCCT 1 cut(s) 537
SsiI CCGC 2 cut(s) 214, 1242
SspI AATATT 2 cut(s) 251, 728
SspMI CTAG 4 cut(s) 86, 554, 791, 1107
StuI AGGCCT 1 cut(s) 537
StyI CCWWGG 3 cut(s) 538, 980, 1151
TaaI ACNGT 4 cut(s) 306, 473, 713, 1025
TaiI ACGT 3 cut(s) 1017, 1134, 1179
TaqI TCGA 6 cut(s) 141, 755, 774, 798, 857, 1179
TasI AATT 9 cut(s) 277, 289, 352, 431, 640, 686, 758, 1115, 1194
TatI WGTACW 1 cut(s) 874
TfiI GAWTC 8 cut(s) 148, 373, 420, 446, 622, 697, 718, 814
Tru1I TTAA 4 cut(s) 356, 645, 1101, 1314
Tru9I TTAA 4 cut(s) 356, 645, 1101, 1314
TscAI CASTG 2 cut(s) 409, 1030
TseFI GTSAC 1 cut(s) 300
TseI GCWGC 5 cut(s) 997, 1320, 1323, 1326, 1329
Tsp45I GTSAC 1 cut(s) 300
TspDTI ATGAA 4 cut(s) 377, 568, 722, 806
TspGWI ACGGA 1 cut(s) 792
TspRI CASTG 2 cut(s) 409, 1030
VpaK11BI GGWCC 2 cut(s) 984, 1257
XapI RAATTY 6 cut(s) 277, 289, 352, 431, 640, 758
XceI RCATGY 1 cut(s) 1171
XspI CTAG 4 cut(s) 86, 554, 791, 1107
ZraI GACGTC 1 cut(s) 1177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.