Rroxscaffold_1G00062530

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011446
Physical Location & Seq
Forward (+)
84634299 .. 84637125
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00062530.1

Sequence Viewer

Length: 1377 bp
ATGGGGAAAGTAACGTCTACTCCTCTCCTCTTTCAATCAAAGCTGGTCTGTTTCTCTCTGCTCTACCTCTTCACCTCTCTGTTTCTAGCTCTCTACACCTCTCTCTCCCAAACCAAATGCCTCTTCAGATCTTCACCTTTCGACCCAATTCAGTCCTCTCTCTTCTCCTACCCTCCCAATTATGGAGAACACAAGTATGCAATCCCAACCCAACGTAAATCTTGCTCTTCCCCTGTCCATTTCTCAGATTATTGGACGGTCTTGAAGGAGATCCAAAATTTGTGCAAGAATTCTTCGTGGAATTCATCTGTTTTGAGTTATATGCAGGGAAAGTCTGAGACTTTTGGAGGGAATTTCAGTACCCAAAATAGGATTTCCTATTTTGTTCATTACAATGAGAGCACTGAAATTCCTTGTGGGTTCCTGAAGAAATTTCCAGTTAGTGATTCTGATAGAATTGTCATGGAAGAGTGTAGAGGACTTGTAGTAGTCTCGGCTATCTTCAATGATCATGATAAAATTCGACAACCCAAGGGGATTGGATCGAAAACCCTTGAGAATGTATGTTTCTTTATGTTTATAGATGAGATAACCCTCAAAGGATTTTACCAGCATCATTTGATTCCAGAGAAATCTGAAAACTTTAAGATTGGTGCATGGCGGGTTGTCAAGGTTTTGAGCAAGGGATTGTATGAGACTCCGGCAATGAATGGTGTGATACCAAAGTACTTGGTTCATAGACTTTTCCCAAACGCCAAATTCAGCATTTGGGTGGATGCGAAAATGCAGCTGATGGTTGATCCATTACTTTTGATTCATACGCTTGTTGTGAATGAGAACGCCGACATGGCGATTTCAAAGCATCCTTACTATGTTCATACTATGGAAGAAGCAATGGCGACGGCGAGGTGGAAGAAATGGTGGGACGTCGATGGATTGAAGAGGCAAATGGAGACTTATTGTGAAAATGGTTTGCTCCCCTGGACTCCTGATAAGCTTCCTTATCCCTCAGATGTACCAGATAGTGCTTTGATTCTGAGGAAACATGGACTGAGGAGCAATCTATTCTCCTGCCTATTGTTTAATGAGATGGAGGCATTCAACCCAAGAGACCAATTAGCCTTTGCATATGTGAGAGACCTCATGAGCCCAAGGCTGAAGCTGAACATGTTTTCAGTTGAAGTGTTTGAGCAAATTGCTGTGGAATATAGGCACAACCTTAAGCCTGGAGTTGGGACCAGTGGACGTGAATCTAAGGGAGGACCCAAAACCAAAAAGACCAAAAGGGCAGACAATGATTTGTTGTATGTCAATGGTAGCTGTTGCAGCAAGTGTCAGAGGTATCTTTTGGAGATGTGGGGGGAATCCCCTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

458

Amino Acids

52.55

Weight (kDa)

8.88

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 110 - 405 1.3e-110 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46220
fragaria_vesca FvH4_3g05570 FvH4_3g05570
malus_domestica MD05G1284000.v1.1 MD10G1262300.v1.1
prunus_persica Prupe.4G080400_v2.0.a1 Prupe.4G080400_v2.0.a1
pyrus_communis pycom05g26310 pycom10g21850
rosa_chinensis RchiOBHm_Chr5g0014101
rosa_laevigata RLG00000032081
rosa_multiflora Rmu_sc0007767.1_g000016
rosa_roxburghii Rroxscaffold_1G00062530
rosa_rugosa Rorug05G0015700
rosa_samantha Rh5AG109600 Rh5BG106400 Rh5BG106500 Rh5CG118300 Rh5DG105100
rosa_wichuraiana Rw5G009570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 930
AccI GTMKAC 1 cut(s) 17
AciI CCGC 1 cut(s) 661
AclWI GGATC 3 cut(s) 265, 550, 794
AcsI RAATTY 8 cut(s) 277, 289, 301, 352, 408, 431, 519, 758
AcuI CTGAAG 3 cut(s) 109, 446, 1178
AcyI GRCGYC 1 cut(s) 927
AfaI GTAC 3 cut(s) 361, 728, 1017
AfiI CCNNNNNNNGG 3 cut(s) 182, 369, 1232
AflII CTTAAG 1 cut(s) 1220
AflIII ACRYGT 1 cut(s) 1167
AgsI TTSAA 7 cut(s) 35, 265, 505, 858, 940, 1102, 1181
AjiI CACGTC 1 cut(s) 1247
AjnI CCWGG 3 cut(s) 980, 1225, 1369
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 6 cut(s) 43, 89, 790, 997, 1162, 1320
AluI AGCT 6 cut(s) 43, 89, 790, 997, 1162, 1320
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 6 cut(s) 332, 496, 689, 947, 1104, 1131
AlwI GGATC 3 cut(s) 265, 550, 794
ApeKI GCWGC 2 cut(s) 787, 1326
ApoI RAATTY 8 cut(s) 277, 289, 301, 352, 408, 431, 519, 758
AspS9I GGNCC 2 cut(s) 1236, 1262
AsuHPI GGTGA 2 cut(s) 64, 126
AvaII GGWCC 2 cut(s) 1236, 1262
BaeI ACNNNNGTAYC 2 cut(s) 710, 743
BanII GRGCYC 1 cut(s) 1151
Bbv12I GWGCWC 1 cut(s) 404
BbvI GCAGC 2 cut(s) 799, 1338
BccI CCATC 3 cut(s) 787, 926, 1084
BceAI ACGGC 1 cut(s) 918
BciT130I CCWGG 3 cut(s) 982, 1227, 1371
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 6 cut(s) 332, 496, 689, 947, 1104, 1131
BfaI CTAG 1 cut(s) 86
BfrI CTTAAG 1 cut(s) 1220
BglI GCCNNNNNGGC 1 cut(s) 848
BglII AGATCT 1 cut(s) 128
BisI GCNGC 2 cut(s) 788, 1327
BlsI GCNGC 2 cut(s) 789, 1328
BmcAI AGTACT 1 cut(s) 728
Bme1390I CCNGG 3 cut(s) 982, 1227, 1371
Bme18I GGWCC 2 cut(s) 1236, 1262
BmgBI CACGTC 1 cut(s) 1247
BmgT120I GGNCC 2 cut(s) 1236, 1262
BmiI GGNNCC 3 cut(s) 422, 1237, 1264
BmrFI CCNGG 3 cut(s) 982, 1227, 1371
BmsI GCATC 3 cut(s) 622, 766, 871
BpmI CTGGAG 1 cut(s) 1248
BpuEI CTTGAG 1 cut(s) 575
BsaHI GRCGYC 1 cut(s) 927
BsaI GGTCTC 2 cut(s) 1104, 1131
BsaJI CCNNGG 4 cut(s) 531, 980, 1151, 1369
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 369, 1232
Bse1I ACTGG 2 cut(s) 437, 1239
Bse3DI GCAATG 2 cut(s) 711, 900
BseBI CCWGG 3 cut(s) 982, 1227, 1371
BseDI CCNNGG 4 cut(s) 531, 980, 1151, 1369
BseGI GGATG 2 cut(s) 781, 862
BseLI CCNNNNNNNGG 3 cut(s) 182, 369, 1232
BseMI GCAATG 2 cut(s) 711, 900
BseMII CTCAG 5 cut(s) 258, 327, 1023, 1028, 1043
BseNI ACTGG 2 cut(s) 437, 1239
BseRI GAGGAG 3 cut(s) 12, 17, 1069
BseXI GCAGC 2 cut(s) 799, 1338
BsiHKAI GWGCWC 1 cut(s) 404
BsiSI CCGG 1 cut(s) 701
BslFI GGGAC 2 cut(s) 938, 1249
BslI CCNNNNNNNGG 3 cut(s) 182, 369, 1232
BsmAI GTCTC 6 cut(s) 332, 496, 689, 947, 1104, 1131
BsmFI GGGAC 2 cut(s) 938, 1249
BsmI GAATGC 1 cut(s) 1097
Bso31I GGTCTC 2 cut(s) 1104, 1131
Bsp1286I GDGCHC 2 cut(s) 404, 1151
Bsp143I GATC 5 cut(s) 128, 270, 508, 542, 799
BspACI CCGC 1 cut(s) 661
BspCNI CTCAG 5 cut(s) 257, 328, 1022, 1029, 1044
BspHI TCATGA 2 cut(s) 511, 1143
BspLI GGNNCC 3 cut(s) 422, 1237, 1264
BspPI GGATC 3 cut(s) 265, 550, 794
BspQI GCTCTTC 1 cut(s) 232
BspTI CTTAAG 1 cut(s) 1220
BspTNI GGTCTC 2 cut(s) 1104, 1131
BsrDI GCAATG 2 cut(s) 711, 900
BsrI ACTGG 2 cut(s) 437, 1239
BssECI CCNNGG 4 cut(s) 531, 980, 1151, 1369
BssMI GATC 5 cut(s) 128, 270, 508, 542, 799
BssNI GRCGYC 1 cut(s) 927
BssT1I CCWWGG 2 cut(s) 531, 1151
Bst2UI CCWGG 3 cut(s) 982, 1227, 1371
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 6 cut(s) 74, 128, 167, 232, 462, 935
BstACI GRCGYC 1 cut(s) 927
BstAFI CTTAAG 1 cut(s) 1220
BstDEI CTNAG 6 cut(s) 244, 336, 1009, 1037, 1052, 1254
BstF5I GGATG 2 cut(s) 781, 862
BstKTI GATC 5 cut(s) 131, 273, 511, 545, 802
BstMAI GTCTC 6 cut(s) 332, 496, 689, 947, 1104, 1131
BstMBI GATC 5 cut(s) 128, 270, 508, 542, 799
BstMWI GCNNNNNNNGC 2 cut(s) 848, 1326
BstNI CCWGG 3 cut(s) 982, 1227, 1371
BstNSI RCATGY 1 cut(s) 1171
BstSCI CCNGG 3 cut(s) 980, 1225, 1369
BstV1I GCAGC 2 cut(s) 799, 1338
BstX2I RGATCY 2 cut(s) 128, 270
BstYI RGATCY 2 cut(s) 128, 270
BtrI CACGTC 1 cut(s) 1247
BtsCI GGATG 2 cut(s) 781, 862
BtsIMutI CAGTG 2 cut(s) 402, 1246
CciI TCATGA 2 cut(s) 511, 1143
Cfr13I GGNCC 2 cut(s) 1236, 1262
Csp6I GTAC 3 cut(s) 360, 727, 1016
CviAII CATG 7 cut(s) 463, 512, 657, 847, 1046, 1144, 1168
CviQI GTAC 3 cut(s) 360, 727, 1016
DdeI CTNAG 6 cut(s) 244, 336, 1009, 1037, 1052, 1254
DpnI GATC 5 cut(s) 130, 272, 510, 544, 801
DpnII GATC 5 cut(s) 128, 270, 508, 542, 799
Eam1104I CTCTTC 6 cut(s) 74, 128, 167, 232, 462, 935
EarI CTCTTC 6 cut(s) 74, 128, 167, 232, 462, 935
Eco130I CCWWGG 2 cut(s) 531, 1151
Eco24I GRGCYC 1 cut(s) 1151
Eco31I GGTCTC 2 cut(s) 1104, 1131
Eco47I GGWCC 2 cut(s) 1236, 1262
Eco57I CTGAAG 3 cut(s) 109, 446, 1178
EcoO109I RGGNCCY 1 cut(s) 1262
EcoRI GAATTC 2 cut(s) 289, 301
EcoRII CCWGG 3 cut(s) 980, 1225, 1369
EcoT14I CCWWGG 2 cut(s) 531, 1151
EcoT38I GRGCYC 1 cut(s) 1151
ErhI CCWWGG 2 cut(s) 531, 1151
FaeI CATG 7 cut(s) 466, 515, 660, 850, 1049, 1147, 1171
FaqI GGGAC 2 cut(s) 938, 1249
FatI CATG 7 cut(s) 462, 511, 656, 846, 1045, 1143, 1167
FauI CCCGC 1 cut(s) 654
FauNDI CATATG 1 cut(s) 1129
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 2 cut(s) 788, 1327
FokI GGATG 2 cut(s) 788, 849
FriOI GRGCYC 1 cut(s) 1151
Fsp4HI GCNGC 2 cut(s) 788, 1327
FspBI CTAG 1 cut(s) 86
GluI GCNGC 2 cut(s) 788, 1327
GsuI CTGGAG 1 cut(s) 1248
HapII CCGG 1 cut(s) 701
Hin1I GRCGYC 1 cut(s) 927
Hin1II CATG 7 cut(s) 466, 515, 660, 850, 1049, 1147, 1171
HindIII AAGCTT 1 cut(s) 995
HinfI GANTC 8 cut(s) 446, 622, 697, 814, 985, 1033, 1250, 1364
HpaII CCGG 1 cut(s) 701
HphI GGTGA 2 cut(s) 64, 126
Hpy166II GTNNAC 2 cut(s) 18, 1244
Hpy188I TCNGA 8 cut(s) 128, 247, 337, 451, 637, 1012, 1038, 1338
Hpy188III TCNNGA 6 cut(s) 262, 424, 512, 626, 989, 1144
Hpy8I GTNNAC 2 cut(s) 18, 1244
Hpy99I CGWCG 2 cut(s) 904, 932
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4IV ACGT 4 cut(s) 14, 214, 927, 1246
HpyCH4V TGCA 7 cut(s) 200, 285, 325, 656, 787, 1127, 1326
HpyF10VI GCNNNNNNNGC 2 cut(s) 848, 1326
HpyF3I CTNAG 6 cut(s) 244, 336, 1009, 1037, 1052, 1254
HpySE526I ACGT 4 cut(s) 14, 214, 927, 1246
Hsp92I GRCGYC 1 cut(s) 927
Hsp92II CATG 7 cut(s) 466, 515, 660, 850, 1049, 1147, 1171
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 5 cut(s) 128, 270, 508, 542, 799
LguI GCTCTTC 1 cut(s) 232
LmnI GCTCC 2 cut(s) 981, 1056
Lsp1109I GCAGC 2 cut(s) 799, 1338
LweI GCATC 3 cut(s) 622, 766, 871
MaeI CTAG 1 cut(s) 86
MaeII ACGT 4 cut(s) 14, 214, 927, 1246
MaeIII GTNAC 1 cut(s) 10
MalI GATC 5 cut(s) 130, 272, 510, 544, 801
MboI GATC 5 cut(s) 128, 270, 508, 542, 799
MflI RGATCY 2 cut(s) 128, 270
MhlI GDGCHC 2 cut(s) 404, 1151
MlyI GAGTC 2 cut(s) 691, 979
MseI TTAA 3 cut(s) 645, 1083, 1221
MslI CAYNNNNRTG 3 cut(s) 195, 393, 770
MspA1I CMGCKG 1 cut(s) 790
MspCI CTTAAG 1 cut(s) 1220
MspI CCGG 1 cut(s) 701
MspR9I CCNGG 3 cut(s) 982, 1227, 1371
Mva1269I GAATGC 1 cut(s) 1097
MvaI CCWGG 3 cut(s) 982, 1227, 1371
MwoI GCNNNNNNNGC 2 cut(s) 848, 1326
NdeI CATATG 1 cut(s) 1129
NdeII GATC 5 cut(s) 128, 270, 508, 542, 799
NlaIII CATG 7 cut(s) 466, 515, 660, 850, 1049, 1147, 1171
NlaIV GGNNCC 3 cut(s) 422, 1237, 1264
NmeAIII GCCGAG 1 cut(s) 473
NspI RCATGY 1 cut(s) 1171
PagI TCATGA 2 cut(s) 511, 1143
PciI ACATGT 1 cut(s) 1167
PciSI GCTCTTC 1 cut(s) 232
PctI GAATGC 1 cut(s) 1097
PfeI GAWTC 6 cut(s) 446, 622, 814, 1033, 1250, 1364
PkrI GCNGC 2 cut(s) 789, 1328
PleI GAGTC 2 cut(s) 691, 979
PpsI GAGTC 2 cut(s) 691, 979
PpuMI RGGWCCY 1 cut(s) 1262
PscI ACATGT 1 cut(s) 1167
Psp5II RGGWCCY 1 cut(s) 1262
Psp6I CCWGG 3 cut(s) 980, 1225, 1369
PspGI CCWGG 3 cut(s) 980, 1225, 1369
PspN4I GGNNCC 3 cut(s) 422, 1237, 1264
PspPI GGNCC 2 cut(s) 1236, 1262
PspPPI RGGWCCY 1 cut(s) 1262
PsuI RGATCY 2 cut(s) 128, 270
PvuII CAGCTG 1 cut(s) 790
RsaI GTAC 3 cut(s) 361, 728, 1017
RsaNI GTAC 3 cut(s) 360, 727, 1016
RseI CAYNNNNRTG 3 cut(s) 195, 393, 770
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 3 cut(s) 645, 1083, 1221
SatI GCNGC 2 cut(s) 788, 1327
Sau3AI GATC 5 cut(s) 128, 270, 508, 542, 799
Sau96I GGNCC 2 cut(s) 1236, 1262
ScaI AGTACT 1 cut(s) 728
SchI GAGTC 2 cut(s) 691, 979
ScrFI CCNGG 3 cut(s) 982, 1227, 1371
SduI GDGCHC 2 cut(s) 404, 1151
SfaNI GCATC 3 cut(s) 622, 766, 871
SinI GGWCC 2 cut(s) 1236, 1262
SmiMI CAYNNNNRTG 3 cut(s) 195, 393, 770
SmlI CTYRAG 2 cut(s) 554, 1220
SmoI CTYRAG 2 cut(s) 554, 1220
SsiI CCGC 1 cut(s) 661
SspMI CTAG 1 cut(s) 86
StyD4I CCNGG 3 cut(s) 980, 1225, 1369
StyI CCWWGG 2 cut(s) 531, 1151
TaaI ACNGT 1 cut(s) 259
TaiI ACGT 4 cut(s) 17, 217, 930, 1249
TaqI TCGA 4 cut(s) 141, 523, 545, 930
TatI WGTACW 1 cut(s) 726
TfiI GAWTC 6 cut(s) 446, 622, 814, 1033, 1250, 1364
Tru1I TTAA 3 cut(s) 645, 1083, 1221
Tru9I TTAA 3 cut(s) 645, 1083, 1221
TscAI CASTG 2 cut(s) 409, 1246
TseI GCWGC 2 cut(s) 787, 1326
TspDTI ATGAA 6 cut(s) 294, 377, 722, 725, 806, 866
TspRI CASTG 2 cut(s) 409, 1246
Vha464I CTTAAG 1 cut(s) 1220
VpaK11BI GGWCC 2 cut(s) 1236, 1262
XapI RAATTY 8 cut(s) 277, 289, 301, 352, 408, 431, 519, 758
XceI RCATGY 1 cut(s) 1171
XmiI GTMKAC 1 cut(s) 17
XspI CTAG 1 cut(s) 86
ZraI GACGTC 1 cut(s) 928
ZrmI AGTACT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.