MD10G1262300.v1.1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011446
Physical Location & Seq
Forward (+)
35682079 .. 35685422
3344 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1262300.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGGGGAAAGCAACGTCTACTCCTCTCTTCTTTCGATCAAAGTTGGTATGTTTCTCTCTGCTCTACCTCTTCACCTCTCTGTTTCTGGCTCTCTACACCTCTCTCTCCCAAACCAAATGCCTCTTCAGAACTTCCCCCTTCGACCCGATTCAGGCCCCTCTCTTCTCCTACCCTTCCACCTACGGCGAGCACAAGTATGCAATCCCAACCCACCGCACCTCTTGCTCTTCCCCCGTCTATTTTTCAGATTATTGGATGGTCTTGAAGGAGATCCAGAATTTGTGTAAGAATTCTTCGTGGTCTCCGTCGGCTTTGAGGTATTTGCAGGGGAAGGCTGAGACGTTTGCAGGAAATTTAAGTACCCAAGAGAGGTTTCCCTATTTTGTTCATTACAATGAGAGCACTGAAATCCCTTGTGGATTCTTGAAGAAATTTCCGATTAGTGATTCTGATCGAAGGGCTATGGAAAACTGTGAAAGAGTCGTAGTGGTTTCGGCGATCTTCAACGACCATGATAAAATCCGACAACCCCGAGGCCTCGGATCGAAAACCCTAGACAATATATGTTTTTTTCTGTTCATAGATGATGTAACCCTGAAGGGACTTGACTACCATAGATTGATTCCGAGAAAATCTAAAAACTTTAAGATCGGTGTGTGGCGAGTAGTGAAAGTTTTGAGCAAGGAATTGTATGAGAATCCTGCAATGAACGGTGTGATACCAAAATATTTAGTCCATAGGCTTTTCCCAAACTCAAAATTCAGCATTTGGATCGACGCGAAGCTGCAGCTAGTGGTCGATCCGTTGCTGTTGATTCATTCACTTCTTGTGTCTGAGAACGCTGACATGGCTATTTCGAAACATCCGTACTATGTACATACTATGGAGGAGGCAATGGCGACGGCGAGGTGGCAGAAATGGAGGGATGTTGATGGGTTGAAGAGGCAGATGGAGGCTTATTGCGAGAATGGATTGCTTCCTTGGACCCCCAGCAAGCTGCCTTATCCCTCAGATGTACCAGACAATGCTCTAATCCTGAGGAAACATGGATTGAGGAGCAACCTATTCTCATGCCTGTTGTTCAATGAGTTGGAGGCATTTAACCCTAGAGACCAGTTGGCTTTTGCATACGTGAGAGACCTCATGAGCCCAAGGCTGAAGCTCAACATGTTTGAGGTCGAAGTGTTTGAGCAAATTGTCGTGGAATACAGGCACAACATCAAGCCTGAAGGTGGGGCCACTGAGAGTCTTGTGGGGTCCAAAGCCCCCAAAACCAGAAATACCAAAAGGGCAAGCCCTGATTTGTTGTATGTCAATGGCAGCTGCTGCAGCAAGTGCCAGAAGTATCTTTTGGAGATGTGGGGTGAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

458

Amino Acids

52.65

Weight (kDa)

9.01

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 111 - 405 2.6e-110 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46220
fragaria_vesca FvH4_3g05570 FvH4_3g05570
malus_domestica MD05G1284000.v1.1 MD10G1262300.v1.1
prunus_persica Prupe.4G080400_v2.0.a1 Prupe.4G080400_v2.0.a1
pyrus_communis pycom05g26310 pycom10g21850
rosa_chinensis RchiOBHm_Chr5g0014101
rosa_laevigata RLG00000032081
rosa_multiflora Rmu_sc0007767.1_g000016
rosa_roxburghii Rroxscaffold_1G00062530
rosa_rugosa Rorug05G0015700
rosa_samantha Rh5AG109600 Rh5BG106400 Rh5BG106500 Rh5CG118300 Rh5DG105100
rosa_wichuraiana Rw5G009570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 17
AccII CGCG 1 cut(s) 779
AciI CCGC 1 cut(s) 214
AclWI GGATC 4 cut(s) 265, 550, 779, 794
AcsI RAATTY 5 cut(s) 277, 289, 352, 431, 758
AcuI CTGAAG 4 cut(s) 109, 617, 1178, 1248
AfaI GTAC 4 cut(s) 361, 869, 876, 1017
AfiI CCNNNNNNNGG 3 cut(s) 151, 369, 1232
AflIII ACRYGT 1 cut(s) 1167
AgsI TTSAA 5 cut(s) 265, 427, 505, 940, 1084
AjuI GAANNNNNNNTTGG 6 cut(s) 107, 139, 357, 389, 1334, 1366
AluBI AGCT 5 cut(s) 784, 790, 997, 1162, 1323
AluI AGCT 5 cut(s) 784, 790, 997, 1162, 1323
Alw21I GWGCWC 2 cut(s) 192, 404
Alw26I GTCTC 4 cut(s) 306, 332, 1104, 1131
AlwI GGATC 4 cut(s) 265, 550, 779, 794
AlwNI CAGNNNCTG 1 cut(s) 1326
Ama87I CYCGRG 1 cut(s) 531
AoxI GGCC 3 cut(s) 153, 535, 1236
ApeKI GCWGC 7 cut(s) 784, 787, 997, 1320, 1323, 1326, 1329
ApoI RAATTY 5 cut(s) 277, 289, 352, 431, 758
AspS9I GGNCC 4 cut(s) 154, 984, 1236, 1257
AsuHPI GGTGA 1 cut(s) 64
AsuII TTCGAA 1 cut(s) 857
AvaI CYCGRG 1 cut(s) 531
AvaII GGWCC 2 cut(s) 984, 1257
AxyI CCTNAGG 1 cut(s) 1037
BanII GRGCYC 1 cut(s) 1151
BarI GAAGNNNNNNTAC 2 cut(s) 277, 309
Bbv12I GWGCWC 2 cut(s) 192, 404
BbvI GCAGC 7 cut(s) 771, 799, 984, 1310, 1313, 1332, 1341
BccI CCATC 3 cut(s) 250, 926, 943
BceAI ACGGC 2 cut(s) 199, 918
BcgI CGANNNNNNTGC 2 cut(s) 754, 788
BcoDI GTCTC 4 cut(s) 306, 332, 1104, 1131
BfaI CTAG 3 cut(s) 554, 791, 1107
BfmI CTRYAG 2 cut(s) 785, 1327
BisI GCNGC 7 cut(s) 785, 788, 998, 1321, 1324, 1327, 1330
BlsI GCNGC 7 cut(s) 786, 789, 999, 1322, 1325, 1328, 1331
Bme18I GGWCC 2 cut(s) 984, 1257
BmeT110I CYCGRG 1 cut(s) 531
BmgT120I GGNCC 4 cut(s) 154, 984, 1236, 1257
BmiI GGNNCC 4 cut(s) 156, 986, 1237, 1258
Bpu14I TTCGAA 1 cut(s) 857
BsaAI YACGTR 1 cut(s) 1132
BsaBI GATNNNNATC 1 cut(s) 450
BsaI GGTCTC 3 cut(s) 306, 1104, 1131
BsaJI CCNNGG 4 cut(s) 532, 538, 980, 1151
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 369, 1232
Bse1I ACTGG 1 cut(s) 1114
Bse21I CCTNAGG 1 cut(s) 1037
Bse3DI GCAATG 2 cut(s) 711, 900
Bse8I GATNNNNATC 1 cut(s) 450
BseDI CCNNGG 4 cut(s) 532, 538, 980, 1151
BseGI GGATG 3 cut(s) 261, 862, 931
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 3 cut(s) 151, 369, 1232
BseMI GCAATG 2 cut(s) 711, 900
BseMII CTCAG 5 cut(s) 327, 825, 1023, 1028, 1233
BseNI ACTGG 1 cut(s) 1114
BseRI GAGGAG 3 cut(s) 12, 902, 1069
BseXI GCAGC 7 cut(s) 771, 799, 984, 1310, 1313, 1332, 1341
BseYI CCCAGC 1 cut(s) 989
Bsh1236I CGCG 1 cut(s) 779
BshFI GGCC 3 cut(s) 155, 537, 1238
BsiHKAI GWGCWC 2 cut(s) 192, 404
BsiHKCI CYCGRG 1 cut(s) 531
BslFI GGGAC 1 cut(s) 615
BslI CCNNNNNNNGG 3 cut(s) 151, 369, 1232
BsmAI GTCTC 4 cut(s) 306, 332, 1104, 1131
BsmBI CGTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 615
BsnI GGCC 3 cut(s) 155, 537, 1238
Bso31I GGTCTC 3 cut(s) 306, 1104, 1131
BsoBI CYCGRG 1 cut(s) 531
Bsp119I TTCGAA 1 cut(s) 857
Bsp1286I GDGCHC 3 cut(s) 192, 404, 1151
Bsp1407I TGTACA 1 cut(s) 874
Bsp143I GATC 8 cut(s) 35, 270, 451, 498, 542, 648, 771, 799
BspACI CCGC 1 cut(s) 214
BspANI GGCC 3 cut(s) 155, 537, 1238
BspCNI CTCAG 5 cut(s) 328, 826, 1022, 1029, 1234
BspFNI CGCG 1 cut(s) 779
BspHI TCATGA 1 cut(s) 1143
BspLI GGNNCC 4 cut(s) 156, 986, 1237, 1258
BspMAI CTGCAG 2 cut(s) 789, 1331
BspPI GGATC 4 cut(s) 265, 550, 779, 794
BspQI GCTCTTC 1 cut(s) 232
BspT104I TTCGAA 1 cut(s) 857
BspTNI GGTCTC 3 cut(s) 306, 1104, 1131
BsrDI GCAATG 2 cut(s) 711, 900
BsrGI TGTACA 1 cut(s) 874
BsrI ACTGG 1 cut(s) 1114
BssECI CCNNGG 4 cut(s) 532, 538, 980, 1151
BssMI GATC 8 cut(s) 35, 270, 451, 498, 542, 648, 771, 799
BssT1I CCWWGG 2 cut(s) 980, 1151
Bst4CI ACNGT 2 cut(s) 473, 713
Bst6I CTCTTC 6 cut(s) 32, 74, 128, 167, 232, 935
BstAPI GCANNNNNTGC 3 cut(s) 222, 1326, 1335
BstAUI TGTACA 1 cut(s) 874
BstBAI YACGTR 1 cut(s) 1132
BstBI TTCGAA 1 cut(s) 857
BstC8I GCNNGC 3 cut(s) 188, 995, 1294
BstDEI CTNAG 5 cut(s) 336, 834, 1009, 1037, 1242
BstF5I GGATG 3 cut(s) 261, 862, 931
BstFNI CGCG 1 cut(s) 779
BstKTI GATC 8 cut(s) 38, 273, 454, 501, 545, 651, 774, 802
BstMAI GTCTC 4 cut(s) 306, 332, 1104, 1131
BstMBI GATC 8 cut(s) 35, 270, 451, 498, 542, 648, 771, 799
BstMWI GCNNNNNNNGC 5 cut(s) 222, 848, 1326, 1329, 1335
BstNSI RCATGY 1 cut(s) 1171
BstSFI CTRYAG 2 cut(s) 785, 1327
BstUI CGCG 1 cut(s) 779
BstV1I GCAGC 7 cut(s) 771, 799, 984, 1310, 1313, 1332, 1341
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
Bsu36I CCTNAGG 1 cut(s) 1037
BsuRI GGCC 3 cut(s) 155, 537, 1238
BtsCI GGATG 3 cut(s) 261, 862, 931
BtsIMutI CAGTG 2 cut(s) 402, 1239
Cac8I GCNNGC 3 cut(s) 188, 995, 1294
CaiI CAGNNNCTG 1 cut(s) 1326
CciI TCATGA 1 cut(s) 1143
Cfr13I GGNCC 4 cut(s) 154, 984, 1236, 1257
CseI GACGC 1 cut(s) 785
Csp6I GTAC 4 cut(s) 360, 868, 875, 1016
CviAII CATG 6 cut(s) 512, 847, 1046, 1071, 1144, 1168
CviQI GTAC 4 cut(s) 360, 868, 875, 1016
DdeI CTNAG 5 cut(s) 336, 834, 1009, 1037, 1242
DpnI GATC 8 cut(s) 37, 272, 453, 500, 544, 650, 773, 801
DpnII GATC 8 cut(s) 35, 270, 451, 498, 542, 648, 771, 799
Eam1104I CTCTTC 6 cut(s) 32, 74, 128, 167, 232, 935
EarI CTCTTC 6 cut(s) 32, 74, 128, 167, 232, 935
Eco130I CCWWGG 2 cut(s) 980, 1151
Eco147I AGGCCT 1 cut(s) 537
Eco24I GRGCYC 1 cut(s) 1151
Eco31I GGTCTC 3 cut(s) 306, 1104, 1131
Eco47I GGWCC 2 cut(s) 984, 1257
Eco57I CTGAAG 4 cut(s) 109, 617, 1178, 1248
Eco81I CCTNAGG 1 cut(s) 1037
Eco88I CYCGRG 1 cut(s) 531
EcoO109I RGGNCCY 1 cut(s) 154
EcoRI GAATTC 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 980, 1151
EcoT38I GRGCYC 1 cut(s) 1151
ErhI CCWWGG 2 cut(s) 980, 1151
Esp3I CGTCTC 1 cut(s) 332
FaeI CATG 6 cut(s) 515, 850, 1049, 1074, 1147, 1171
FaqI GGGAC 1 cut(s) 615
FatI CATG 6 cut(s) 511, 846, 1045, 1070, 1143, 1167
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 7 cut(s) 785, 788, 998, 1321, 1324, 1327, 1330
FokI GGATG 3 cut(s) 268, 849, 938
FriOI GRGCYC 1 cut(s) 1151
Fsp4HI GCNGC 7 cut(s) 785, 788, 998, 1321, 1324, 1327, 1330
FspBI CTAG 3 cut(s) 554, 791, 1107
GluI GCNGC 7 cut(s) 785, 788, 998, 1321, 1324, 1327, 1330
GsaI CCCAGC 1 cut(s) 993
HaeIII GGCC 3 cut(s) 155, 537, 1238
HgaI GACGC 1 cut(s) 785
Hin1II CATG 6 cut(s) 515, 850, 1049, 1074, 1147, 1171
HinfI GANTC 9 cut(s) 148, 420, 446, 480, 622, 697, 814, 1246, 1367
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 1 cut(s) 18
Hpy188I TCNGA 9 cut(s) 128, 247, 438, 451, 524, 542, 627, 835, 1012
Hpy188III TCNNGA 5 cut(s) 262, 274, 424, 1036, 1144
Hpy8I GTNNAC 1 cut(s) 18
Hpy99I CGWCG 3 cut(s) 310, 779, 904
HpyAV CCTTC 7 cut(s) 148, 183, 259, 325, 450, 592, 1223
HpyCH4III ACNGT 2 cut(s) 473, 713
HpyCH4IV ACGT 3 cut(s) 14, 341, 1131
HpyCH4V TGCA 7 cut(s) 200, 325, 347, 704, 787, 1127, 1329
HpyF10VI GCNNNNNNNGC 5 cut(s) 222, 848, 1326, 1329, 1335
HpyF3I CTNAG 5 cut(s) 336, 834, 1009, 1037, 1242
HpySE526I ACGT 3 cut(s) 14, 341, 1131
Hsp92II CATG 6 cut(s) 515, 850, 1049, 1074, 1147, 1171
Kzo9I GATC 8 cut(s) 35, 270, 451, 498, 542, 648, 771, 799
LguI GCTCTTC 1 cut(s) 232
LmnI GCTCC 1 cut(s) 1056
Lsp1109I GCAGC 7 cut(s) 771, 799, 984, 1310, 1313, 1332, 1341
MaeI CTAG 3 cut(s) 554, 791, 1107
MaeII ACGT 3 cut(s) 14, 341, 1131
MaeIII GTNAC 1 cut(s) 589
MalI GATC 8 cut(s) 37, 272, 453, 500, 544, 650, 773, 801
MboI GATC 8 cut(s) 35, 270, 451, 498, 542, 648, 771, 799
MboII GAAGA 9 cut(s) 19, 61, 115, 154, 219, 285, 439, 493, 952
MflI RGATCY 1 cut(s) 270
MhlI GDGCHC 3 cut(s) 192, 404, 1151
MluCI AATT 7 cut(s) 277, 289, 352, 431, 686, 758, 1194
MlyI GAGTC 2 cut(s) 489, 1255
MmeI TCCRAC 2 cut(s) 547, 1071
MseI TTAA 3 cut(s) 356, 645, 1101
MslI CAYNNNNRTG 2 cut(s) 195, 393
MspA1I CMGCKG 1 cut(s) 1323
MvnI CGCG 1 cut(s) 779
MwoI GCNNNNNNNGC 5 cut(s) 222, 848, 1326, 1329, 1335
NdeII GATC 8 cut(s) 35, 270, 451, 498, 542, 648, 771, 799
NlaIII CATG 6 cut(s) 515, 850, 1049, 1074, 1147, 1171
NlaIV GGNNCC 4 cut(s) 156, 986, 1237, 1258
NspI RCATGY 1 cut(s) 1171
NspV TTCGAA 1 cut(s) 857
PagI TCATGA 1 cut(s) 1143
PceI AGGCCT 1 cut(s) 537
PciI ACATGT 1 cut(s) 1167
PciSI GCTCTTC 1 cut(s) 232
PcsI WCGNNNNNNNCGW 2 cut(s) 302, 863
PfeI GAWTC 7 cut(s) 148, 420, 446, 622, 697, 814, 1367
PkrI GCNGC 7 cut(s) 786, 789, 999, 1322, 1325, 1328, 1331
PleI GAGTC 2 cut(s) 488, 1254
PpsI GAGTC 2 cut(s) 488, 1254
Ppu21I YACGTR 1 cut(s) 1132
PscI ACATGT 1 cut(s) 1167
PspFI CCCAGC 1 cut(s) 989
PspN4I GGNNCC 4 cut(s) 156, 986, 1237, 1258
PspPI GGNCC 4 cut(s) 154, 984, 1236, 1257
PstI CTGCAG 2 cut(s) 789, 1331
PstNI CAGNNNCTG 1 cut(s) 1326
PsuI RGATCY 1 cut(s) 270
PvuII CAGCTG 1 cut(s) 1323
RsaI GTAC 4 cut(s) 361, 869, 876, 1017
RsaNI GTAC 4 cut(s) 360, 868, 875, 1016
RseI CAYNNNNRTG 2 cut(s) 195, 393
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 3 cut(s) 356, 645, 1101
SatI GCNGC 7 cut(s) 785, 788, 998, 1321, 1324, 1327, 1330
Sau3AI GATC 8 cut(s) 35, 270, 451, 498, 542, 648, 771, 799
Sau96I GGNCC 4 cut(s) 154, 984, 1236, 1257
SchI GAGTC 2 cut(s) 489, 1255
SduI GDGCHC 3 cut(s) 192, 404, 1151
SfcI CTRYAG 2 cut(s) 785, 1327
SfuI TTCGAA 1 cut(s) 857
SinI GGWCC 2 cut(s) 984, 1257
SmiMI CAYNNNNRTG 2 cut(s) 195, 393
Sse9I AATT 7 cut(s) 277, 289, 352, 431, 686, 758, 1194
SseBI AGGCCT 1 cut(s) 537
SsiI CCGC 1 cut(s) 214
SspI AATATT 1 cut(s) 728
SspMI CTAG 3 cut(s) 554, 791, 1107
StuI AGGCCT 1 cut(s) 537
StyI CCWWGG 2 cut(s) 980, 1151
TaaI ACNGT 2 cut(s) 473, 713
TaiI ACGT 3 cut(s) 17, 344, 1134
TaqI TCGA 8 cut(s) 34, 141, 454, 545, 774, 798, 857, 1179
TasI AATT 7 cut(s) 277, 289, 352, 431, 686, 758, 1194
TatI WGTACW 1 cut(s) 874
TfiI GAWTC 7 cut(s) 148, 420, 446, 622, 697, 814, 1367
Tru1I TTAA 3 cut(s) 356, 645, 1101
Tru9I TTAA 3 cut(s) 356, 645, 1101
TscAI CASTG 2 cut(s) 409, 1246
TseI GCWGC 7 cut(s) 784, 787, 997, 1320, 1323, 1326, 1329
TspDTI ATGAA 4 cut(s) 377, 568, 722, 806
TspGWI ACGGA 3 cut(s) 294, 792, 855
TspRI CASTG 2 cut(s) 409, 1246
VpaK11BI GGWCC 2 cut(s) 984, 1257
XapI RAATTY 5 cut(s) 277, 289, 352, 431, 758
XceI RCATGY 1 cut(s) 1171
XmiI GTMKAC 1 cut(s) 17
XspI CTAG 3 cut(s) 554, 791, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.