Rh5DG105100

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011446
Physical Location & Seq
Reverse (-)
9545300 .. 9548472
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG105100.1

Sequence Viewer

Length: 1092 bp
ATGTGTGACATTGTGAACAGAATAAAGAAGAGTATAAAATCAAAGGCGAGCATCCTTAAGAAATGTGTCATGGGGAAAGTAACGTCCACTCCTCTGCTCTTTCAATCAAAGCTGGTCTGTTTCTCTCTGCTCTACCTCTTCACCTCTCTGTTTCTAGCTCTCTACACCTCTCTCTCCCAAACCAAATGCCTCTTCAGATCTTCACCTTTCGACCCAATTCAGCCCCCTCTCTTCTCCTACCCTCCCAATTATGGAGAACACAAGTATGCAATCCCAACCCAACGTAAATCTTGCTCTTCCCCTGTCCATTTCTCAGATTATTGGAAGGTCTTGAAGGAGATCCAAAATTTGTGCAAGAATTCTTCGTGGAATTCATCGGTTTTGAGTTATATGCAGGGAAAGTCTGAGACTTTTGGAGGGAATTTCAGTACCCAAAATAGGATTTCCTATTTTGTTCATTACAATGAGAGCACTGAAATCCCTTGTGGGTTCCTAAAGAAATTTCCAGTCAGTCATTCTGATAGAATTGTCATGGAAGAGTGTAGAGGACTTGTAGTAGTCTCGGCTATCTTCAATGATCATGATAAAATTCGACAACCCAAGGGGATTGGATCGAAAACCCTTGAGAATGTATGTTTCTTTATGTTTATAGATGAGATAACCCTCAAAGGATTTTACCAGCATCATTTGATTCCAGAAAAATCTGAAAACTATAGGATTGGTGCATGGAGGGTTGTCAAGGTTTTGAGCAAGGGATTGTATGAGAATCCGGCAATGAATGGTGTGATACCAAAGTACTTGGTTCATAGACTTTTCCCAAACGCCAAATTCAGCATTTGGGTGGATGCGAAAATGCAGCTGATGGTTGATCCATTACTTTTGATTCACACACTTGTTGTGAATGAGAACGCCGACATGGCGATTTCAAAGCATCCTTACTATGTTCATACTATGGAAGAAGCAATGGCGACGGCGAGGTGGAAGAAATGGCGGGACGTTGATGGATTAAAGAGGCAAATGGAGACATATTGTGAAAATGGTTTGCTCCCCTGGACTCCTGATAAGCTTCCTTATCCCTCAGGTATTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

363

Amino Acids

41.81

Weight (kDa)

9.26

Isoelectric Point (pI)

32.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 133 - 361 1.3e-79 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46220
fragaria_vesca FvH4_3g05570 FvH4_3g05570
malus_domestica MD05G1284000.v1.1 MD10G1262300.v1.1
prunus_persica Prupe.4G080400_v2.0.a1 Prupe.4G080400_v2.0.a1
pyrus_communis pycom05g26310 pycom10g21850
rosa_chinensis RchiOBHm_Chr5g0014101
rosa_laevigata RLG00000032081
rosa_multiflora Rmu_sc0007767.1_g000016
rosa_roxburghii Rroxscaffold_1G00062530
rosa_rugosa Rorug05G0015700
rosa_samantha Rh5AG109600 Rh5BG106400 Rh5BG106500 Rh5CG118300 Rh5DG105100
rosa_wichuraiana Rw5G009570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 991
AclWI GGATC 3 cut(s) 334, 619, 863
AcsI RAATTY 7 cut(s) 346, 358, 370, 421, 500, 588, 827
AcuI CTGAAG 1 cut(s) 178
AfaI GTAC 2 cut(s) 430, 797
AfiI CCNNNNNNNGG 2 cut(s) 251, 438
AflII CTTAAG 1 cut(s) 56
AgsI TTSAA 4 cut(s) 104, 334, 574, 927
AjnI CCWGG 1 cut(s) 1049
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AluBI AGCT 4 cut(s) 112, 158, 859, 1066
AluI AGCT 4 cut(s) 112, 158, 859, 1066
Alw21I GWGCWC 1 cut(s) 473
Alw26I GTCTC 3 cut(s) 401, 565, 1016
AlwI GGATC 3 cut(s) 334, 619, 863
ApeKI GCWGC 1 cut(s) 856
ApoI RAATTY 7 cut(s) 346, 358, 370, 421, 500, 588, 827
AsuHPI GGTGA 2 cut(s) 133, 195
AxyI CCTNAGG 1 cut(s) 1078
BaeI ACNNNNGTAYC 2 cut(s) 779, 812
Bbv12I GWGCWC 1 cut(s) 473
BbvI GCAGC 1 cut(s) 868
BccI CCATC 2 cut(s) 856, 995
BceAI ACGGC 1 cut(s) 987
BciT130I CCWGG 1 cut(s) 1051
BclI TGATCA 1 cut(s) 577
BcoDI GTCTC 3 cut(s) 401, 565, 1016
BfaI CTAG 1 cut(s) 155
BfmI CTRYAG 1 cut(s) 712
BfrI CTTAAG 1 cut(s) 56
BglI GCCNNNNNGGC 1 cut(s) 917
BglII AGATCT 1 cut(s) 197
BisI GCNGC 1 cut(s) 857
BlsI GCNGC 1 cut(s) 858
BmcAI AGTACT 1 cut(s) 797
Bme1390I CCNGG 1 cut(s) 1051
BmiI GGNNCC 1 cut(s) 491
BmrFI CCNGG 1 cut(s) 1051
BmsI GCATC 4 cut(s) 60, 691, 835, 940
BpuEI CTTGAG 1 cut(s) 644
BsaJI CCNNGG 2 cut(s) 600, 1049
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 438
Bse1I ACTGG 1 cut(s) 506
Bse21I CCTNAGG 1 cut(s) 1078
Bse3DI GCAATG 2 cut(s) 780, 969
BseBI CCWGG 1 cut(s) 1051
BseDI CCNNGG 2 cut(s) 600, 1049
BseGI GGATG 3 cut(s) 51, 850, 931
BseLI CCNNNNNNNGG 2 cut(s) 251, 438
BseMI GCAATG 2 cut(s) 780, 969
BseMII CTCAG 3 cut(s) 327, 396, 1092
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 81
BseXI GCAGC 1 cut(s) 868
BsiHKAI GWGCWC 1 cut(s) 473
BsiSI CCGG 1 cut(s) 770
BslFI GGGAC 1 cut(s) 1007
BslI CCNNNNNNNGG 2 cut(s) 251, 438
BsmAI GTCTC 3 cut(s) 401, 565, 1016
BsmFI GGGAC 1 cut(s) 1007
Bsp1286I GDGCHC 1 cut(s) 473
Bsp143I GATC 5 cut(s) 197, 339, 577, 611, 868
BspACI CCGC 1 cut(s) 991
BspCNI CTCAG 3 cut(s) 326, 397, 1091
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 1 cut(s) 491
BspPI GGATC 3 cut(s) 334, 619, 863
BspQI GCTCTTC 1 cut(s) 301
BspTI CTTAAG 1 cut(s) 56
BsrDI GCAATG 2 cut(s) 780, 969
BsrI ACTGG 1 cut(s) 506
BssECI CCNNGG 2 cut(s) 600, 1049
BssMI GATC 5 cut(s) 197, 339, 577, 611, 868
BssT1I CCWWGG 1 cut(s) 600
Bst2UI CCWGG 1 cut(s) 1051
Bst6I CTCTTC 6 cut(s) 23, 143, 197, 236, 301, 531
BstAFI CTTAAG 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 3 cut(s) 313, 405, 1078
BstF5I GGATG 3 cut(s) 51, 850, 931
BstKTI GATC 5 cut(s) 200, 342, 580, 614, 871
BstMAI GTCTC 3 cut(s) 401, 565, 1016
BstMBI GATC 5 cut(s) 197, 339, 577, 611, 868
BstMWI GCNNNNNNNGC 1 cut(s) 917
BstNI CCWGG 1 cut(s) 1051
BstSCI CCNGG 1 cut(s) 1049
BstSFI CTRYAG 1 cut(s) 712
BstV1I GCAGC 1 cut(s) 868
BstX2I RGATCY 2 cut(s) 197, 339
BstYI RGATCY 2 cut(s) 197, 339
Bsu36I CCTNAGG 1 cut(s) 1078
BtsCI GGATG 3 cut(s) 51, 850, 931
BtsIMutI CAGTG 1 cut(s) 471
Cac8I GCNNGC 1 cut(s) 49
CciI TCATGA 1 cut(s) 580
Csp6I GTAC 2 cut(s) 429, 796
CviAII CATG 5 cut(s) 70, 532, 581, 726, 916
CviJI RGCY 6 cut(s) 112, 158, 223, 566, 859, 1066
CviKI_1 RGCY 6 cut(s) 112, 158, 223, 566, 859, 1066
CviQI GTAC 2 cut(s) 429, 796
DdeI CTNAG 3 cut(s) 313, 405, 1078
DpnI GATC 5 cut(s) 199, 341, 579, 613, 870
DpnII GATC 5 cut(s) 197, 339, 577, 611, 868
Eam1104I CTCTTC 6 cut(s) 23, 143, 197, 236, 301, 531
EarI CTCTTC 6 cut(s) 23, 143, 197, 236, 301, 531
Eco130I CCWWGG 1 cut(s) 600
Eco57I CTGAAG 1 cut(s) 178
Eco81I CCTNAGG 1 cut(s) 1078
EcoRI GAATTC 2 cut(s) 358, 370
EcoRII CCWGG 1 cut(s) 1049
EcoT14I CCWWGG 1 cut(s) 600
ErhI CCWWGG 1 cut(s) 600
FaeI CATG 5 cut(s) 73, 535, 584, 729, 919
FaqI GGGAC 1 cut(s) 1007
FatI CATG 5 cut(s) 69, 531, 580, 725, 915
FauI CCCGC 1 cut(s) 984
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 1 cut(s) 857
FokI GGATG 3 cut(s) 38, 857, 918
Fsp4HI GCNGC 1 cut(s) 857
FspBI CTAG 1 cut(s) 155
GluI GCNGC 1 cut(s) 857
HapII CCGG 1 cut(s) 770
Hin1II CATG 5 cut(s) 73, 535, 584, 729, 919
HindIII AAGCTT 1 cut(s) 1064
HinfI GANTC 4 cut(s) 691, 766, 883, 1054
HpaII CCGG 1 cut(s) 770
HphI GGTGA 2 cut(s) 133, 195
Hpy166II GTNNAC 2 cut(s) 16, 87
Hpy188I TCNGA 5 cut(s) 197, 316, 406, 520, 706
Hpy188III TCNNGA 4 cut(s) 331, 581, 695, 1058
Hpy8I GTNNAC 2 cut(s) 16, 87
Hpy99I CGWCG 1 cut(s) 973
HpyAV CCTTC 2 cut(s) 319, 328
HpyCH4IV ACGT 3 cut(s) 83, 283, 996
HpyCH4V TGCA 5 cut(s) 269, 354, 394, 725, 856
HpyF10VI GCNNNNNNNGC 1 cut(s) 917
HpyF3I CTNAG 3 cut(s) 313, 405, 1078
HpySE526I ACGT 3 cut(s) 83, 283, 996
Hsp92II CATG 5 cut(s) 73, 535, 584, 729, 919
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 5 cut(s) 197, 339, 577, 611, 868
LguI GCTCTTC 1 cut(s) 301
LmnI GCTCC 1 cut(s) 1050
Lsp1109I GCAGC 1 cut(s) 868
LweI GCATC 4 cut(s) 60, 691, 835, 940
MaeI CTAG 1 cut(s) 155
MaeII ACGT 3 cut(s) 83, 283, 996
MaeIII GTNAC 2 cut(s) 5, 79
MalI GATC 5 cut(s) 199, 341, 579, 613, 870
MboI GATC 5 cut(s) 197, 339, 577, 611, 868
MflI RGATCY 2 cut(s) 197, 339
MhlI GDGCHC 1 cut(s) 473
MlyI GAGTC 1 cut(s) 1048
MseI TTAA 2 cut(s) 57, 1007
MslI CAYNNNNRTG 3 cut(s) 264, 462, 839
MspA1I CMGCKG 1 cut(s) 859
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 1 cut(s) 770
MspR9I CCNGG 1 cut(s) 1051
MvaI CCWGG 1 cut(s) 1051
MwoI GCNNNNNNNGC 1 cut(s) 917
NdeII GATC 5 cut(s) 197, 339, 577, 611, 868
NlaIII CATG 5 cut(s) 73, 535, 584, 729, 919
NlaIV GGNNCC 1 cut(s) 491
NmeAIII GCCGAG 1 cut(s) 542
NmuCI GTSAC 1 cut(s) 5
PagI TCATGA 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 301
PfeI GAWTC 3 cut(s) 691, 766, 883
PkrI GCNGC 1 cut(s) 858
PleI GAGTC 1 cut(s) 1048
PpsI GAGTC 1 cut(s) 1048
Psp6I CCWGG 1 cut(s) 1049
PspGI CCWGG 1 cut(s) 1049
PspN4I GGNNCC 1 cut(s) 491
PsuI RGATCY 2 cut(s) 197, 339
PvuII CAGCTG 1 cut(s) 859
RsaI GTAC 2 cut(s) 430, 797
RsaNI GTAC 2 cut(s) 429, 796
RseI CAYNNNNRTG 3 cut(s) 264, 462, 839
SapI GCTCTTC 1 cut(s) 301
SaqAI TTAA 2 cut(s) 57, 1007
SatI GCNGC 1 cut(s) 857
Sau3AI GATC 5 cut(s) 197, 339, 577, 611, 868
ScaI AGTACT 1 cut(s) 797
SchI GAGTC 1 cut(s) 1048
ScrFI CCNGG 1 cut(s) 1051
SduI GDGCHC 1 cut(s) 473
SfaNI GCATC 4 cut(s) 60, 691, 835, 940
SfcI CTRYAG 1 cut(s) 712
SmiMI CAYNNNNRTG 3 cut(s) 264, 462, 839
SmlI CTYRAG 2 cut(s) 56, 623
SmoI CTYRAG 2 cut(s) 56, 623
SsiI CCGC 1 cut(s) 991
SspMI CTAG 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 1049
StyI CCWWGG 1 cut(s) 600
TaiI ACGT 3 cut(s) 86, 286, 999
TaqI TCGA 3 cut(s) 210, 592, 614
TatI WGTACW 1 cut(s) 795
TfiI GAWTC 3 cut(s) 691, 766, 883
Tru1I TTAA 2 cut(s) 57, 1007
Tru9I TTAA 2 cut(s) 57, 1007
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 1 cut(s) 856
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 5 cut(s) 363, 446, 791, 794, 935
TspRI CASTG 1 cut(s) 478
Vha464I CTTAAG 1 cut(s) 56
XapI RAATTY 7 cut(s) 346, 358, 370, 421, 500, 588, 827
XspI CTAG 1 cut(s) 155
ZrmI AGTACT 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.