FvH4_3g05570

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011446
Physical Location & Seq
Forward (+)
3267092 .. 3269919
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05570.t2

Sequence Viewer

Length: 1428 bp
ATGAATAGAATAAAGAGACGGAAGAAGGCGAGCATTCTTAAGAAACGTGTTATGGGGAAAGTAACGTCCACTCCTCTGCTCTTTCAATCAAAGCTGGTCTGTTTCTCTCTGCTCTACCTCTTCACCTCTCTGTTTCTAGCTCTATACACCTCTCTCTCCCAATCCAAATGCCTCTTCAGATCTTCACCTTTCGATCCAATTCAGACCCCTCTCTTCTCCTACCCTCCCAATTATGGAGAACACAAGTATGCTATCCCAACCCAACGTAAAACTTGCTCCTCCCGTGTCTATTTCTCAGATTATTGGAAAGTCTTGAAGGAGATCCAGAATTTGTGCAAGACTTCTTCGTGGAACTCGTCAGTTTTGAGGTATAAGCAGGGAAAGTCTGAGACTTTTGGAGGGAATTTCAGTACCCAAAATAGGATTTCTTATTTTGTTCATTACAATGAGAGCACTGAAGTCCCTTGTGGGTTCCTGAAGAAATTTCCAGTTAATGACTCTGATAGAACTGCGATGGAAGAGTGTAAAGGACTAGTAGTAGTCTCGGCTATATTCAATGATCATGATAAAATTCGACAACCAAAGGGGATTGGATCGAAAACCCTTGAGAATGTATGTTTCTTTATGTTTATAGATGAGATAACCCTCAAAGGATTTTACAAGCATCATTTGATTCCAGAAAAATCCGAAAACTATAAGATTGGCGCATGGAGGGTTGTCAAGGTTATGAGCAAGGGATTGTATGAGAATCCGGCAATGAATGGTGTGATACCAAAGTATTTAGTTCATAGACTTTTCCCAAACGCCAAATTCAGCATTTGGGTGGATGCAAAAATGCAGCTGATGGTTGATCCATTACTTTTGATTCATACACTAGTTGTGAATGAGAACGCCGACATGGCGATTTCAAGGCATCCTTACTATGTTCATACTATGGAAGAAGCAATGGCGACGGCGAGGTGGAAGAAATGGTGGGACGTTGATGGATTGAAGAGCCAAATGGAGACTTACTGTGAGAATGGTTTGCGCCCTTGGAATCCTGATAAGCTTCCATATCCCTCAGATGTACCAGATAGTGCTGTGATTCTGAGGAAACATGGCCTGAGGAGCAATCTATTCTCCTGCCTATTGTTTAATGAGATGGAGGCATTCAACCCAAGAGACCAATTAGCCTTTGCATATGTGAGAGACCTCATGAGCCCAAGGCTGAAGCTGAACATGTTTCCAGTTGAAGTGTTTGAGCAAATTGCTGTGGAATATAGGCACAACCTTAAGCCTGGAGTTGAAACCAGTGGAGGTGAATCTAAAGGAGGGCCCCAAACCAAAAACACCAAAAGGGCAGGCAATGATTTGTTGTATGTCAATGGCAGCTGCTGCAGTAAGTGCCAGATGTATCTTTTGGAGATGTGGGGGGAATCCCCTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

476

Amino Acids

54.73

Weight (kDa)

9.32

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 125 - 355 2.5e-81 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46220
fragaria_vesca FvH4_3g05570 FvH4_3g05570
malus_domestica MD05G1284000.v1.1 MD10G1262300.v1.1
prunus_persica Prupe.4G080400_v2.0.a1 Prupe.4G080400_v2.0.a1
pyrus_communis pycom05g26310 pycom10g21850
rosa_chinensis RchiOBHm_Chr5g0014101
rosa_laevigata RLG00000032081
rosa_multiflora Rmu_sc0007767.1_g000016
rosa_roxburghii Rroxscaffold_1G00062530
rosa_rugosa Rorug05G0015700
rosa_samantha Rh5AG109600 Rh5BG106400 Rh5BG106500 Rh5CG118300 Rh5DG105100
rosa_wichuraiana Rw5G009570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 188, 316, 601, 845
AcsI RAATTY 5 cut(s) 328, 403, 482, 570, 809
AcuI CTGAAG 4 cut(s) 160, 477, 497, 1229
AfaI GTAC 2 cut(s) 412, 1068
AfiI CCNNNNNNNGG 2 cut(s) 233, 420
AflII CTTAAG 2 cut(s) 38, 1271
AflIII ACRYGT 2 cut(s) 46, 1218
AgsI TTSAA 8 cut(s) 86, 316, 556, 909, 991, 1153, 1232, 1286
AhlI ACTAGT 2 cut(s) 532, 874
AjnI CCWGG 2 cut(s) 1276, 1420
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AluBI AGCT 6 cut(s) 94, 140, 841, 1048, 1213, 1371
AluI AGCT 6 cut(s) 94, 140, 841, 1048, 1213, 1371
Alw21I GWGCWC 1 cut(s) 455
Alw26I GTCTC 6 cut(s) 10, 383, 547, 998, 1155, 1182
AlwI GGATC 4 cut(s) 188, 316, 601, 845
AlwNI CAGNNNCTG 1 cut(s) 1374
AoxI GGCC 2 cut(s) 1099, 1313
ApaI GGGCCC 1 cut(s) 1317
ApeKI GCWGC 4 cut(s) 838, 1368, 1371, 1374
ApoI RAATTY 5 cut(s) 328, 403, 482, 570, 809
AspLEI GCGC 2 cut(s) 707, 1029
AspS9I GGNCC 2 cut(s) 1313, 1314
AsuHPI GGTGA 3 cut(s) 115, 177, 1310
AxyI CCTNAGG 1 cut(s) 1103
BaeGI GKGCMC 1 cut(s) 1317
BaeI ACNNNNGTAYC 2 cut(s) 761, 794
BanII GRGCYC 2 cut(s) 1202, 1317
Bbv12I GWGCWC 1 cut(s) 455
BbvI GCAGC 4 cut(s) 850, 1358, 1361, 1380
BccI CCATC 4 cut(s) 508, 838, 977, 1135
BceAI ACGGC 1 cut(s) 969
BciT130I CCWGG 2 cut(s) 1278, 1422
BclI TGATCA 1 cut(s) 559
BcoDI GTCTC 6 cut(s) 10, 383, 547, 998, 1155, 1182
BcuI ACTAGT 2 cut(s) 532, 874
BfaI CTAG 3 cut(s) 137, 533, 875
BfmI CTRYAG 1 cut(s) 1375
BfrI CTTAAG 2 cut(s) 38, 1271
BglI GCCNNNNNGGC 1 cut(s) 899
BglII AGATCT 1 cut(s) 179
BisI GCNGC 4 cut(s) 839, 1369, 1372, 1375
BlsI GCNGC 4 cut(s) 840, 1370, 1373, 1376
Bme1390I CCNGG 2 cut(s) 1278, 1422
BmgT120I GGNCC 2 cut(s) 1313, 1314
BmiI GGNNCC 3 cut(s) 473, 1315, 1316
BmrFI CCNGG 2 cut(s) 1278, 1422
BmsI GCATC 3 cut(s) 673, 817, 922
BpmI CTGGAG 1 cut(s) 1299
BpuEI CTTGAG 1 cut(s) 626
BsaI GGTCTC 2 cut(s) 1155, 1182
BsaJI CCNNGG 3 cut(s) 1031, 1202, 1420
BsaXI ACNNNNNCTCC 2 cut(s) 55, 85
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 420
Bse1I ACTGG 3 cut(s) 488, 1226, 1290
Bse21I CCTNAGG 1 cut(s) 1103
Bse3DI GCAATG 3 cut(s) 762, 951, 1351
BseBI CCWGG 2 cut(s) 1278, 1422
BseDI CCNNGG 3 cut(s) 1031, 1202, 1420
BseGI GGATG 2 cut(s) 832, 913
BseLI CCNNNNNNNGG 2 cut(s) 233, 420
BseMI GCAATG 3 cut(s) 762, 951, 1351
BseMII CTCAG 5 cut(s) 309, 378, 1074, 1079, 1094
BseNI ACTGG 3 cut(s) 488, 1226, 1290
BseRI GAGGAG 3 cut(s) 63, 268, 1120
BseSI GKGCMC 1 cut(s) 1317
BseXI GCAGC 4 cut(s) 850, 1358, 1361, 1380
BshFI GGCC 2 cut(s) 1101, 1315
BsiHKAI GWGCWC 1 cut(s) 455
BsiSI CCGG 1 cut(s) 752
BslFI GGGAC 2 cut(s) 446, 989
BslI CCNNNNNNNGG 2 cut(s) 233, 420
BsmAI GTCTC 6 cut(s) 10, 383, 547, 998, 1155, 1182
BsmBI CGTCTC 1 cut(s) 10
BsmFI GGGAC 2 cut(s) 446, 989
BsmI GAATGC 2 cut(s) 33, 1148
BsnI GGCC 2 cut(s) 1101, 1315
Bso31I GGTCTC 2 cut(s) 1155, 1182
Bsp120I GGGCCC 1 cut(s) 1313
Bsp1286I GDGCHC 3 cut(s) 455, 1202, 1317
Bsp143I GATC 6 cut(s) 179, 193, 321, 559, 593, 850
BspANI GGCC 2 cut(s) 1101, 1315
BspCNI CTCAG 5 cut(s) 308, 379, 1073, 1080, 1095
BspHI TCATGA 2 cut(s) 562, 1194
BspLI GGNNCC 3 cut(s) 473, 1315, 1316
BspMAI CTGCAG 1 cut(s) 1379
BspPI GGATC 4 cut(s) 188, 316, 601, 845
BspQI GCTCTTC 1 cut(s) 986
BspTI CTTAAG 2 cut(s) 38, 1271
BspTNI GGTCTC 2 cut(s) 1155, 1182
BsrDI GCAATG 3 cut(s) 762, 951, 1351
BsrI ACTGG 3 cut(s) 488, 1226, 1290
BssECI CCNNGG 3 cut(s) 1031, 1202, 1420
BssMI GATC 6 cut(s) 179, 193, 321, 559, 593, 850
BssT1I CCWWGG 2 cut(s) 1031, 1202
Bst2UI CCWGG 2 cut(s) 1278, 1422
Bst4CI ACNGT 1 cut(s) 1013
Bst6I CTCTTC 5 cut(s) 125, 179, 218, 513, 986
BstAFI CTTAAG 2 cut(s) 38, 1271
BstAPI GCANNNNNTGC 2 cut(s) 1374, 1383
BstC8I GCNNGC 2 cut(s) 31, 1342
BstDEI CTNAG 5 cut(s) 295, 387, 1060, 1088, 1103
BstF5I GGATG 2 cut(s) 832, 913
BstHHI GCGC 2 cut(s) 707, 1029
BstKTI GATC 6 cut(s) 182, 196, 324, 562, 596, 853
BstMAI GTCTC 6 cut(s) 10, 383, 547, 998, 1155, 1182
BstMBI GATC 6 cut(s) 179, 193, 321, 559, 593, 850
BstMWI GCNNNNNNNGC 4 cut(s) 899, 1107, 1374, 1383
BstNI CCWGG 2 cut(s) 1278, 1422
BstNSI RCATGY 1 cut(s) 1222
BstSCI CCNGG 2 cut(s) 1276, 1420
BstSFI CTRYAG 1 cut(s) 1375
BstSLI GKGCMC 1 cut(s) 1317
BstV1I GCAGC 4 cut(s) 850, 1358, 1361, 1380
BstX2I RGATCY 2 cut(s) 179, 321
BstYI RGATCY 2 cut(s) 179, 321
Bsu36I CCTNAGG 1 cut(s) 1103
BsuRI GGCC 2 cut(s) 1101, 1315
BtgZI GCGATG 1 cut(s) 527
BtsCI GGATG 2 cut(s) 832, 913
BtsIMutI CAGTG 2 cut(s) 453, 1297
Cac8I GCNNGC 2 cut(s) 31, 1342
CaiI CAGNNNCTG 1 cut(s) 1374
CciI TCATGA 2 cut(s) 562, 1194
CfoI GCGC 2 cut(s) 707, 1029
Cfr13I GGNCC 2 cut(s) 1313, 1314
Csp6I GTAC 2 cut(s) 411, 1067
CviAII CATG 6 cut(s) 563, 708, 898, 1097, 1195, 1219
CviQI GTAC 2 cut(s) 411, 1067
DdeI CTNAG 5 cut(s) 295, 387, 1060, 1088, 1103
DpnI GATC 6 cut(s) 181, 195, 323, 561, 595, 852
DpnII GATC 6 cut(s) 179, 193, 321, 559, 593, 850
Eam1104I CTCTTC 5 cut(s) 125, 179, 218, 513, 986
EarI CTCTTC 5 cut(s) 125, 179, 218, 513, 986
Eco130I CCWWGG 2 cut(s) 1031, 1202
Eco24I GRGCYC 2 cut(s) 1202, 1317
Eco31I GGTCTC 2 cut(s) 1155, 1182
Eco57I CTGAAG 4 cut(s) 160, 477, 497, 1229
Eco81I CCTNAGG 1 cut(s) 1103
EcoO109I RGGNCCY 2 cut(s) 1313, 1314
EcoRII CCWGG 2 cut(s) 1276, 1420
EcoT14I CCWWGG 2 cut(s) 1031, 1202
EcoT38I GRGCYC 2 cut(s) 1202, 1317
ErhI CCWWGG 2 cut(s) 1031, 1202
Esp3I CGTCTC 1 cut(s) 10
FaeI CATG 6 cut(s) 566, 711, 901, 1100, 1198, 1222
FalI AAGNNNNNCTT 2 cut(s) 901, 933
FaqI GGGAC 2 cut(s) 446, 989
FatI CATG 6 cut(s) 562, 707, 897, 1096, 1194, 1218
FauNDI CATATG 1 cut(s) 1180
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 4 cut(s) 839, 1369, 1372, 1375
FokI GGATG 2 cut(s) 839, 900
FriOI GRGCYC 2 cut(s) 1202, 1317
Fsp4HI GCNGC 4 cut(s) 839, 1369, 1372, 1375
FspBI CTAG 3 cut(s) 137, 533, 875
GlaI GCGC 2 cut(s) 706, 1028
GluI GCNGC 4 cut(s) 839, 1369, 1372, 1375
GsuI CTGGAG 1 cut(s) 1299
HaeIII GGCC 2 cut(s) 1101, 1315
HapII CCGG 1 cut(s) 752
HhaI GCGC 2 cut(s) 707, 1029
Hin1II CATG 6 cut(s) 566, 711, 901, 1100, 1198, 1222
Hin6I GCGC 2 cut(s) 705, 1027
HinP1I GCGC 2 cut(s) 705, 1027
HindIII AAGCTT 1 cut(s) 1046
HinfI GANTC 8 cut(s) 497, 673, 748, 865, 1036, 1084, 1301, 1415
HpaII CCGG 1 cut(s) 752
HphI GGTGA 3 cut(s) 115, 177, 1310
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 8 cut(s) 179, 204, 298, 388, 502, 688, 1063, 1089
Hpy188III TCNNGA 7 cut(s) 313, 325, 475, 563, 677, 1040, 1195
Hpy8I GTNNAC 1 cut(s) 69
Hpy99I CGWCG 1 cut(s) 955
HpyAV CCTTC 2 cut(s) 19, 310
HpyCH4III ACNGT 1 cut(s) 1013
HpyCH4IV ACGT 4 cut(s) 46, 65, 265, 978
HpyCH4V TGCA 5 cut(s) 336, 830, 838, 1178, 1377
HpyF10VI GCNNNNNNNGC 4 cut(s) 899, 1107, 1374, 1383
HpyF3I CTNAG 5 cut(s) 295, 387, 1060, 1088, 1103
HpySE526I ACGT 4 cut(s) 46, 65, 265, 978
Hsp92II CATG 6 cut(s) 566, 711, 901, 1100, 1198, 1222
HspAI GCGC 2 cut(s) 705, 1027
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 6 cut(s) 179, 193, 321, 559, 593, 850
LguI GCTCTTC 1 cut(s) 986
LmnI GCTCC 2 cut(s) 281, 1107
Lsp1109I GCAGC 4 cut(s) 850, 1358, 1361, 1380
LweI GCATC 3 cut(s) 673, 817, 922
MaeI CTAG 3 cut(s) 137, 533, 875
MaeII ACGT 4 cut(s) 46, 65, 265, 978
MaeIII GTNAC 1 cut(s) 61
MalI GATC 6 cut(s) 181, 195, 323, 561, 595, 852
MboI GATC 6 cut(s) 179, 193, 321, 559, 593, 850
MflI RGATCY 2 cut(s) 179, 321
MhlI GDGCHC 3 cut(s) 455, 1202, 1317
MluCI AATT 9 cut(s) 198, 229, 328, 403, 482, 570, 809, 1166, 1245
MlyI GAGTC 1 cut(s) 491
MseI TTAA 4 cut(s) 39, 492, 1134, 1272
MslI CAYNNNNRTG 3 cut(s) 246, 444, 821
MspA1I CMGCKG 2 cut(s) 841, 1371
MspCI CTTAAG 2 cut(s) 38, 1271
MspI CCGG 1 cut(s) 752
MspR9I CCNGG 2 cut(s) 1278, 1422
Mva1269I GAATGC 2 cut(s) 33, 1148
MvaI CCWGG 2 cut(s) 1278, 1422
MwoI GCNNNNNNNGC 4 cut(s) 899, 1107, 1374, 1383
NdeI CATATG 1 cut(s) 1180
NdeII GATC 6 cut(s) 179, 193, 321, 559, 593, 850
NlaIII CATG 6 cut(s) 566, 711, 901, 1100, 1198, 1222
NlaIV GGNNCC 3 cut(s) 473, 1315, 1316
NmeAIII GCCGAG 1 cut(s) 524
NspI RCATGY 1 cut(s) 1222
PagI TCATGA 2 cut(s) 562, 1194
PciI ACATGT 1 cut(s) 1218
PciSI GCTCTTC 1 cut(s) 986
PcsI WCGNNNNNNNCGW 1 cut(s) 353
PctI GAATGC 2 cut(s) 33, 1148
PfeI GAWTC 7 cut(s) 673, 748, 865, 1036, 1084, 1301, 1415
PkrI GCNGC 4 cut(s) 840, 1370, 1373, 1376
PleI GAGTC 1 cut(s) 491
PpsI GAGTC 1 cut(s) 491
PscI ACATGT 1 cut(s) 1218
Psp6I CCWGG 2 cut(s) 1276, 1420
PspGI CCWGG 2 cut(s) 1276, 1420
PspN4I GGNNCC 3 cut(s) 473, 1315, 1316
PspOMI GGGCCC 1 cut(s) 1313
PspPI GGNCC 2 cut(s) 1313, 1314
PstI CTGCAG 1 cut(s) 1379
PstNI CAGNNNCTG 1 cut(s) 1374
PsuI RGATCY 2 cut(s) 179, 321
PvuII CAGCTG 2 cut(s) 841, 1371
RsaI GTAC 2 cut(s) 412, 1068
RsaNI GTAC 2 cut(s) 411, 1067
RseI CAYNNNNRTG 3 cut(s) 246, 444, 821
SapI GCTCTTC 1 cut(s) 986
SaqAI TTAA 4 cut(s) 39, 492, 1134, 1272
SatI GCNGC 4 cut(s) 839, 1369, 1372, 1375
Sau3AI GATC 6 cut(s) 179, 193, 321, 559, 593, 850
Sau96I GGNCC 2 cut(s) 1313, 1314
SchI GAGTC 1 cut(s) 491
ScrFI CCNGG 2 cut(s) 1278, 1422
SduI GDGCHC 3 cut(s) 455, 1202, 1317
SfaNI GCATC 3 cut(s) 673, 817, 922
SfcI CTRYAG 1 cut(s) 1375
SmiMI CAYNNNNRTG 3 cut(s) 246, 444, 821
SmlI CTYRAG 3 cut(s) 38, 605, 1271
SmoI CTYRAG 3 cut(s) 38, 605, 1271
SpeI ACTAGT 2 cut(s) 532, 874
Sse9I AATT 9 cut(s) 198, 229, 328, 403, 482, 570, 809, 1166, 1245
SspMI CTAG 3 cut(s) 137, 533, 875
StyD4I CCNGG 2 cut(s) 1276, 1420
StyI CCWWGG 2 cut(s) 1031, 1202
TaaI ACNGT 1 cut(s) 1013
TaiI ACGT 4 cut(s) 49, 68, 268, 981
TaqI TCGA 3 cut(s) 192, 574, 596
TasI AATT 9 cut(s) 198, 229, 328, 403, 482, 570, 809, 1166, 1245
TfiI GAWTC 7 cut(s) 673, 748, 865, 1036, 1084, 1301, 1415
Tru1I TTAA 4 cut(s) 39, 492, 1134, 1272
Tru9I TTAA 4 cut(s) 39, 492, 1134, 1272
TscAI CASTG 2 cut(s) 460, 1297
TseI GCWGC 4 cut(s) 838, 1368, 1371, 1374
TspDTI ATGAA 6 cut(s) 17, 428, 773, 776, 857, 917
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 2 cut(s) 460, 1297
Vha464I CTTAAG 2 cut(s) 38, 1271
XapI RAATTY 5 cut(s) 328, 403, 482, 570, 809
XceI RCATGY 1 cut(s) 1222
XspI CTAG 3 cut(s) 137, 533, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.